WO2000016610A1 - Procede permettant d'induire une organogenese directe et in vitro dans les oignons - Google Patents
Procede permettant d'induire une organogenese directe et in vitro dans les oignons Download PDFInfo
- Publication number
- WO2000016610A1 WO2000016610A1 PCT/SI1999/000022 SI9900022W WO0016610A1 WO 2000016610 A1 WO2000016610 A1 WO 2000016610A1 SI 9900022 W SI9900022 W SI 9900022W WO 0016610 A1 WO0016610 A1 WO 0016610A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- media
- induction
- differentiation
- contain
- process according
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 40
- 235000002732 Allium cepa var. cepa Nutrition 0.000 title claims abstract description 27
- 230000005305 organ development Effects 0.000 title claims abstract description 20
- 238000000338 in vitro Methods 0.000 title claims abstract description 13
- 241000234282 Allium Species 0.000 title claims abstract 3
- 230000006698 induction Effects 0.000 title claims description 64
- 210000001672 ovary Anatomy 0.000 claims abstract description 13
- 230000000392 somatic effect Effects 0.000 claims abstract description 7
- 230000000408 embryogenic effect Effects 0.000 claims abstract description 5
- 230000004069 differentiation Effects 0.000 claims description 34
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- 239000004062 cytokinin Substances 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 230000000888 organogenic effect Effects 0.000 claims description 17
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- 229930006000 Sucrose Natural products 0.000 claims description 16
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- 229930192334 Auxin Natural products 0.000 claims description 12
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- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 7
- 239000003349 gelling agent Substances 0.000 claims description 7
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- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 6
- 239000005595 Picloram Substances 0.000 claims description 6
- 150000001720 carbohydrates Chemical class 0.000 claims description 6
- 235000014633 carbohydrates Nutrition 0.000 claims description 6
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 6
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- 229960000367 inositol Drugs 0.000 claims description 6
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- 229930003231 vitamin Natural products 0.000 claims description 6
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 5
- 230000012010 growth Effects 0.000 claims description 5
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 4
- GCUVWBFOTMJVLI-UHFFFAOYSA-N 2-(3-methylbut-3-enyl)-7h-purin-6-amine Chemical compound CC(=C)CCC1=NC(N)=C2NC=NC2=N1 GCUVWBFOTMJVLI-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 4
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 claims description 4
- 239000000451 gelidium spp. gum Substances 0.000 claims description 4
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 230000023753 dehiscence Effects 0.000 claims description 3
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- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 229940077731 carbohydrate nutrients Drugs 0.000 claims description 2
- 230000000977 initiatory effect Effects 0.000 claims description 2
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- 230000021749 root development Effects 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
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- 241000196324 Embryophyta Species 0.000 abstract description 13
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- 239000007640 basal medium Substances 0.000 description 3
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- 238000004519 manufacturing process Methods 0.000 description 2
- 241000589158 Agrobacterium Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000948268 Meda Species 0.000 description 1
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- 238000011065 in-situ storage Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
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- 239000006870 ms-medium Substances 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU57685/99A AU5768599A (en) | 1998-09-24 | 1999-09-22 | A process for the induction of direct (in vitro) organogenesis in onion |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SI9800247A SI20053A (sl) | 1998-09-24 | 1998-09-24 | Postopek za indukcijo neposredne in vitro organogeneze pri čebuli |
SIP-9800247 | 1998-09-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000016610A1 true WO2000016610A1 (fr) | 2000-03-30 |
Family
ID=20432337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SI1999/000022 WO2000016610A1 (fr) | 1998-09-24 | 1999-09-22 | Procede permettant d'induire une organogenese directe et in vitro dans les oignons |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU5768599A (fr) |
SI (1) | SI20053A (fr) |
WO (1) | WO2000016610A1 (fr) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6995016B2 (en) | 2000-08-17 | 2006-02-07 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Process for inducing direct somatic embryogenesis in immature scutella cells of pooideae, and rapidly regenerating fertile plants |
CN102870674A (zh) * | 2012-09-05 | 2013-01-16 | 广州白云华南生物科技有限公司 | 一种红葱组培快速繁殖方法 |
EP2925739A4 (fr) * | 2012-11-28 | 2016-07-27 | Stichting Dienst Landbouwkundi | Dihydropyridines substituées pour l'embryogenèse somatique dans des plantes |
CN110226517A (zh) * | 2019-06-26 | 2019-09-13 | 北京市农林科学院 | 一种洋葱离体再生的方法及其使用的培养基 |
CN117441617A (zh) * | 2023-12-22 | 2024-01-26 | 北京花乡花木集团有限公司 | 一种北葱的组织培养方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0814166A2 (fr) * | 1989-08-09 | 1997-12-29 | DeKalb Genetics Corporation | Méthode et compositions pour la préparation de plantes monocotylédones fertiles ainsi que leurs cellules transformées de manière stabile |
-
1998
- 1998-09-24 SI SI9800247A patent/SI20053A/sl unknown
-
1999
- 1999-09-22 AU AU57685/99A patent/AU5768599A/en not_active Abandoned
- 1999-09-22 WO PCT/SI1999/000022 patent/WO2000016610A1/fr active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0814166A2 (fr) * | 1989-08-09 | 1997-12-29 | DeKalb Genetics Corporation | Méthode et compositions pour la préparation de plantes monocotylédones fertiles ainsi que leurs cellules transformées de manière stabile |
Non-Patent Citations (5)
Title |
---|
COHAT, J.: "Obtention chez l'échalote (Allium cepa L var aggregantum) de plantes haploïdes gynogénétiques par culture in vitro de boutons floraux", AGRONOMIE, vol. 14, 1994, pages 299 - 304, XP000879486 * |
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1992, CAMPION, B. ET AL.: "Advances in haploid plant induction in onion (Allium cepa L.) through in vitro gynogenesis", XP002131955 * |
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1995, BOHANEC, B.: "Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants.", XP002131954 * |
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1996, JAKSE, M. ET AL.: "Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants.", XP002131953 * |
DATABASE CAB CAB INTERNATIONAL, WALLINGFORD, OXON, GB; 1997, JEONG HAEBOONG ET AL.: "Plant redifferentiation and in vitro multiplication of onion by shoot primodium culture", XP002131956 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6995016B2 (en) | 2000-08-17 | 2006-02-07 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Process for inducing direct somatic embryogenesis in immature scutella cells of pooideae, and rapidly regenerating fertile plants |
CN102870674A (zh) * | 2012-09-05 | 2013-01-16 | 广州白云华南生物科技有限公司 | 一种红葱组培快速繁殖方法 |
CN102870674B (zh) * | 2012-09-05 | 2013-12-25 | 广州白云华南生物科技有限公司 | 一种红葱组培快速繁殖方法 |
EP2925739A4 (fr) * | 2012-11-28 | 2016-07-27 | Stichting Dienst Landbouwkundi | Dihydropyridines substituées pour l'embryogenèse somatique dans des plantes |
CN110226517A (zh) * | 2019-06-26 | 2019-09-13 | 北京市农林科学院 | 一种洋葱离体再生的方法及其使用的培养基 |
CN110226517B (zh) * | 2019-06-26 | 2021-06-01 | 北京市农林科学院 | 一种洋葱离体再生的方法及其使用的培养基 |
CN117441617A (zh) * | 2023-12-22 | 2024-01-26 | 北京花乡花木集团有限公司 | 一种北葱的组织培养方法 |
CN117441617B (zh) * | 2023-12-22 | 2024-04-02 | 北京花乡花木集团有限公司 | 一种北葱的组织培养方法 |
Also Published As
Publication number | Publication date |
---|---|
AU5768599A (en) | 2000-04-10 |
SI20053A (sl) | 2000-04-30 |
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