SI20053A - Postopek za indukcijo neposredne in vitro organogeneze pri čebuli - Google Patents
Postopek za indukcijo neposredne in vitro organogeneze pri čebuli Download PDFInfo
- Publication number
- SI20053A SI20053A SI9800247A SI9800247A SI20053A SI 20053 A SI20053 A SI 20053A SI 9800247 A SI9800247 A SI 9800247A SI 9800247 A SI9800247 A SI 9800247A SI 20053 A SI20053 A SI 20053A
- Authority
- SI
- Slovenia
- Prior art keywords
- induction
- medium
- media
- differentiation
- shoots
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 62
- 235000002732 Allium cepa var. cepa Nutrition 0.000 title claims abstract description 31
- 230000005305 organ development Effects 0.000 title claims abstract description 16
- 238000000338 in vitro Methods 0.000 title claims abstract description 14
- 241000234282 Allium Species 0.000 title claims abstract 3
- 230000006698 induction Effects 0.000 title claims description 57
- 210000001672 ovary Anatomy 0.000 claims abstract description 31
- 230000000888 organogenic effect Effects 0.000 claims abstract description 26
- 230000008569 process Effects 0.000 claims abstract description 16
- 239000002609 medium Substances 0.000 claims description 56
- 230000004069 differentiation Effects 0.000 claims description 34
- 239000000203 mixture Substances 0.000 claims description 19
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 18
- 239000004062 cytokinin Substances 0.000 claims description 18
- 229930006000 Sucrose Natural products 0.000 claims description 17
- 239000005720 sucrose Substances 0.000 claims description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 16
- 229920002148 Gellan gum Polymers 0.000 claims description 14
- 239000000216 gellan gum Substances 0.000 claims description 13
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- NQQVFXUMIDALNH-UHFFFAOYSA-N picloram Chemical compound NC1=C(Cl)C(Cl)=NC(C(O)=O)=C1Cl NQQVFXUMIDALNH-UHFFFAOYSA-N 0.000 claims description 8
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 7
- 150000001720 carbohydrates Chemical class 0.000 claims description 7
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- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 6
- 239000005595 Picloram Substances 0.000 claims description 6
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 claims description 6
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 6
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 6
- 229960000367 inositol Drugs 0.000 claims description 6
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 6
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- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
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- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 4
- HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 229960002429 proline Drugs 0.000 claims description 4
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000005648 plant growth regulator Substances 0.000 claims description 3
- 206010053567 Coagulopathies Diseases 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 229940077731 carbohydrate nutrients Drugs 0.000 claims description 2
- 230000035602 clotting Effects 0.000 claims description 2
- 239000003630 growth substance Substances 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 229960004793 sucrose Drugs 0.000 claims description 2
- 239000003205 fragrance Substances 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- WYXYBEOWQBGFQB-UHFFFAOYSA-N n-benzyl-7h-purin-2-amine Chemical compound N=1C=C2NC=NC2=NC=1NCC1=CC=CC=C1 WYXYBEOWQBGFQB-UHFFFAOYSA-N 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract description 21
- 230000008929 regeneration Effects 0.000 abstract description 16
- 238000011069 regeneration method Methods 0.000 abstract description 16
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 206010020649 Hyperkeratosis Diseases 0.000 abstract description 11
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
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- 230000000408 embryogenic effect Effects 0.000 abstract description 2
- 210000000056 organ Anatomy 0.000 abstract description 2
- 244000291564 Allium cepa Species 0.000 description 32
- 230000000694 effects Effects 0.000 description 21
- 238000005755 formation reaction Methods 0.000 description 19
- 206010016803 Fluid overload Diseases 0.000 description 8
- 238000011282 treatment Methods 0.000 description 5
- 235000005255 Allium cepa Nutrition 0.000 description 4
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000011419 induction treatment Methods 0.000 description 4
- 210000002257 embryonic structure Anatomy 0.000 description 3
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- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 2
- 229930195732 phytohormone Natural products 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000004043 responsiveness Effects 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241001214984 Crinum thaianum Species 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000656145 Thyrsites atun Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000009629 growth pathway Effects 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000004848 polyfunctional curative Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000030118 somatic embryogenesis Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 230000009772 tissue formation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000009417 vegetative reproduction Effects 0.000 description 1
- 238000013466 vegetative reproduction Methods 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SI9800247A SI20053A (sl) | 1998-09-24 | 1998-09-24 | Postopek za indukcijo neposredne in vitro organogeneze pri čebuli |
| PCT/SI1999/000022 WO2000016610A1 (fr) | 1998-09-24 | 1999-09-22 | Procede permettant d'induire une organogenese directe et in vitro dans les oignons |
| AU57685/99A AU5768599A (en) | 1998-09-24 | 1999-09-22 | A process for the induction of direct (in vitro) organogenesis in onion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SI9800247A SI20053A (sl) | 1998-09-24 | 1998-09-24 | Postopek za indukcijo neposredne in vitro organogeneze pri čebuli |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SI20053A true SI20053A (sl) | 2000-04-30 |
Family
ID=20432337
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI9800247A SI20053A (sl) | 1998-09-24 | 1998-09-24 | Postopek za indukcijo neposredne in vitro organogeneze pri čebuli |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU5768599A (fr) |
| SI (1) | SI20053A (fr) |
| WO (1) | WO2000016610A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6995016B2 (en) | 2000-08-17 | 2006-02-07 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Process for inducing direct somatic embryogenesis in immature scutella cells of pooideae, and rapidly regenerating fertile plants |
| CN102870674B (zh) * | 2012-09-05 | 2013-12-25 | 广州白云华南生物科技有限公司 | 一种红葱组培快速繁殖方法 |
| US20150296779A1 (en) * | 2012-11-28 | 2015-10-22 | Stichting Dienst Landbouwkundig Onderzoek | Substituted dihydropyridines for somatic embryogenesis in plants |
| CN110226517B (zh) * | 2019-06-26 | 2021-06-01 | 北京市农林科学院 | 一种洋葱离体再生的方法及其使用的培养基 |
| CN117441617B (zh) * | 2023-12-22 | 2024-04-02 | 北京花乡花木集团有限公司 | 一种北葱的组织培养方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991002071A2 (fr) * | 1989-08-09 | 1991-02-21 | Dekalb Plant Genetics | Procedes et compositions de production de plantes monocotyledones fecondes ainsi que de leurs cellules transformees de maniere stable |
-
1998
- 1998-09-24 SI SI9800247A patent/SI20053A/sl unknown
-
1999
- 1999-09-22 AU AU57685/99A patent/AU5768599A/en not_active Abandoned
- 1999-09-22 WO PCT/SI1999/000022 patent/WO2000016610A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000016610A1 (fr) | 2000-03-30 |
| AU5768599A (en) | 2000-04-10 |
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