WO2000008440A1 - Calibration device for an optical microtiter plate reading apparatus - Google Patents

Calibration device for an optical microtiter plate reading apparatus Download PDF

Info

Publication number
WO2000008440A1
WO2000008440A1 PCT/EP1999/005579 EP9905579W WO0008440A1 WO 2000008440 A1 WO2000008440 A1 WO 2000008440A1 EP 9905579 W EP9905579 W EP 9905579W WO 0008440 A1 WO0008440 A1 WO 0008440A1
Authority
WO
WIPO (PCT)
Prior art keywords
calibration device
microtiter plate
section
reading apparatus
slope
Prior art date
Application number
PCT/EP1999/005579
Other languages
English (en)
French (fr)
Inventor
Thomas Augustinus Maria Beumer
Wilhelmus Marinus Carpay
Original Assignee
Akzo Nobel N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel N.V. filed Critical Akzo Nobel N.V.
Priority to AU55096/99A priority Critical patent/AU5509699A/en
Publication of WO2000008440A1 publication Critical patent/WO2000008440A1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G01N21/278—Constitution of standards

Definitions

  • the present invention relates to a calibration device for an optical microtiter plate reading apparatus, which calibration device comprises a plurality of optically transparent areas having solid outer surfaces, said areas being dist ⁇ ubbed over the calibration device to be functionally readable by the microtiter plate reading apparatus
  • the object of the present invention is to provide a calibration device that more specifically determines the sensitivity of a plate reader for a further source of error
  • the calibration device according to the present invention is characterized in that at least one area comp ⁇ ses a section having a beam of light diverging property
  • the properties of the beam of light used in an optical plate reading apparatus may have a profound influence on the accuracy of absorption values obtained with the reading apparatus
  • the light beam is not collimated perfectly
  • the beam may be partially deflected by the meniscus of an aqueous liquid in a microtiter plate which is read using the plate reader after performing an colo ⁇ met ⁇ c assay
  • the present invention provides an improved calibration device the device being suitable for indicating of the plate reading apparatus for meniscus effects
  • the calibration device comprising areas with beam of light diverging properties can more accurately assess (periodically) the quality of plate reading apparatus for performing certain assavs
  • the calibration device mav establish that a particular plate reader is suitable for a particular assay, but not for another assay
  • the beam of light diverging property mav be achieved in one of several wa ⁇ s such as internal or superficial light scattering surface a grating (or hologram) etc
  • the calibration plate generally will comprise 96 wells.
  • a rectangular frame that can be fitted with one or more strips comprising the section similar to the strips used for performing an assay in a limited number of wells.
  • the section comprises a concavely curved surface.
  • a concavely curved surface can easily be used to mimic the meniscus of the aqueous solution which is read using the plate reader for a particular assay.
  • the section is substantially circle symmetrical. This allows for more accurately mimicking the meniscus.
  • n P - ni refractive index of the aqueous liquid
  • n p refractive index of the solid material
  • Si is the slope of an aqueous liquid in a well of a microtiter plate.
  • a calibration device that can accurately mimic the etfect of the meniscus of an aqueous liquid such as read for a particular assay.
  • the tangent line has a slope (S p ) substantially equal to or larger than the slope according to formula I, said aqueous liquid being an aqueous liquid showing an extremely concave surface as may be encountered with a particular assay performed in a microtiter plate and using the microtiter plate reading apparatus.
  • ndevice is the refractive index of the material used for the area of the section; ni.quid is the refractive index of a meniscus forming liquid; and ⁇ .qu u i is the approximated radius displayed by liquid in a well of a microtiter plate
  • Calibration devices having this curvature can easily be manufactured, for example using injection moulding, and are a good approximation of the curvature of the meniscus of the aqueous liquid read for a particular assay. Again, a smaller r allows for a higher sensitivity for further sources of error.
  • the section is provided with a coloured component like a dye.
  • a typical example can be waxoline gelb (Waxoline gelb 2GP-FW, ICI), which has an equivalent absorbent spectrum compared to TMB (3-3'5-5'-tetramethylbenzidine) which is a well-known and frequent used compound in the art.
  • Other dyes are Sudan Gelb 150 (BASF, 7178152), Orasol geel (2RLN. CIBA GEIGY), and Ceres geel (3G, Bayer)
  • other dyes can also be used and are therefore within the scope of the present invention.
  • the area of the calibration plate comprises a sandwich of two materials having different refractive indices, the surface being an internal surface
  • a calibration plate can be provided which has a smooth and planar upper and lower surface, making it easy to clean when soiled
  • smooth surfaces can be achieved by fitting a plate comprising curved wells with a flat cover
  • a meniscus emulation tool f.i a frame, e.g a metal frame with holes into which readily available lenses of the proper geometry and focal length can be mounted
  • a frame e.g a metal frame with holes into which readily available lenses of the proper geometry and focal length can be mounted
  • the calibration device comprises at least two areas at different heights.
  • Fig. 1 shows the results of the measurements (492 nm) of the readers Reader 510 compared to SpectraMax with the calibration device (polymer).
  • Fig. 2 shows the results of the measurements (492 nm) of the readers Reader 510 compared to SpectraMax with a plate containing pepton-buffered Saline solution with Tween 20 (PBS/Tween solution).
  • Fig. 3 shows the results of the measurements (492 nm) of the readers Reader 530 compared to SpectraMax with the calibration device (polymer).
  • Fig. 4 shows the results of the measurements (492 nm) of the readers Reader 530 compared to SpectraMax with a plate containing PBS/Tween solutioa
  • a calibration device was manufactured having dimensions (length, width and height) similar to a standard microtiter plate with 96 concave areas at the corresponding locations.
  • the calibration device was made by pouring a mixture of polyester moulding resin (MEK(methylketonperoxide)-giethars) and hardener
  • Two microtiter plates were used as controls to emulate two extreme types of meniscus as may result after performing an immunoassay.
  • the first was filled with milli-Q water, and the second plate was filled with pepton-buf ered Saline solution with 0 1 % Tween 20 (PBS/Tween)(highly concave liquid surface)
  • PBS/Tween pepton-buf ered Saline solution with 0 1 % Tween 20
  • the solution showed no meniscus
  • a highly concaved liquid-air interface with a radius in the order of 5 ⁇ 0, 1 mm (f 15 ⁇ 0,3 mm) formed
  • the amount of liquid in the microtiter plates was 0, 25, 50, to 300 microliter All plates were analyzed using a SpectraMax reader (Molecular Devices, Palo Alto, CA), a Reader-510 (Organon Teknika, Boxtel, the Netherlands) and a Reader-530 (Organon Teknika, Boxtel, the Netherlands)
  • Fig. 1 - 4 show the data for plates containing PBS/Tween and the calibration plate which are measured with three readers, i.e. Reader 510 and Reader 530 (Organon Teknika, Boxtel, The
  • pre-rotation indices mean that the wells are measured by the plate readers before rotation of 180°
  • post-rotation means measurement of the wells by the plate readers after rotation of 180°.
  • Fig 1 shows the results of the measurements (492 nm) of the readers Reader 510 compared to SpectraMax with the calibration device (polymer)
  • the Reader 510 gives the highest absorption values
  • Fig 2 shows the results of the measurements (492 nm) of the readers Reader 510 compared to SpectraMax with a plate containing PBS/Tween solution
  • Reader 510 gives the highest absorption values which is comparable to figure 1
  • Fig. 3 shows the results of the measurements (492 nm) of the readers Reader 530 compared to SpectraMax with the calibration device (polymer)
  • the Reader 530 gives the highest absorption values
  • Fig. 4 shows the results of the measurements (492 nm) of the readers Reader 530 compared to SpectraMax with a plate containing PBS/Tween solution.
  • Reader 530 gives the highest absorption values, which is comparable to figure 3.

Landscapes

  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Optical Measuring Cells (AREA)

Abstract

The present invention relates to calibration device for an optical microtiter plate reading apparatus, such as used for Enzyme Linked Immuno Sorbent Assays. The calibration device comprises a plurality of optically transparent areas having solid outer surfaces. According to the invention at least one area comprises a section having a beam of light diverging property, such as a concavely curved surface. The calibration device allows for accessing the quality of a plate reader for performing certain (immuno)assays.
PCT/EP1999/005579 1998-08-07 1999-08-02 Calibration device for an optical microtiter plate reading apparatus WO2000008440A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU55096/99A AU5509699A (en) 1998-08-07 1999-08-02 Calibration device for an optical microtiter plate reading apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98202667 1998-08-07
NL98202667.6 1998-08-07

Publications (1)

Publication Number Publication Date
WO2000008440A1 true WO2000008440A1 (de) 2000-02-17

Family

ID=8234018

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/005579 WO2000008440A1 (de) 1998-08-07 1999-08-02 Calibration device for an optical microtiter plate reading apparatus

Country Status (2)

Country Link
AU (1) AU5509699A (de)
WO (1) WO2000008440A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6842241B2 (en) 2001-09-04 2005-01-11 Wallac Oy Method for checking the operation of an optical measuring device and checking device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4030699C1 (de) * 1990-09-28 1991-10-10 Bruker Analytische Messtechnik Gmbh, 7512 Rheinstetten, De
US5183761A (en) * 1989-07-21 1993-02-02 Freeman Mary J Method of making calibration solution for verifying calibration and linearity of vertical photometers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5183761A (en) * 1989-07-21 1993-02-02 Freeman Mary J Method of making calibration solution for verifying calibration and linearity of vertical photometers
DE4030699C1 (de) * 1990-09-28 1991-10-10 Bruker Analytische Messtechnik Gmbh, 7512 Rheinstetten, De

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6842241B2 (en) 2001-09-04 2005-01-11 Wallac Oy Method for checking the operation of an optical measuring device and checking device

Also Published As

Publication number Publication date
AU5509699A (en) 2000-02-28

Similar Documents

Publication Publication Date Title
CN101548162B (zh) 紧凑的反射折射分光计
AU603233B2 (en) Readhead for reflectance measurement of distant samples
US7101660B2 (en) Method for producing a colorimetric resonant reflection biosensor on rigid surfaces
US5989923A (en) Hologram containing sensor
US7175980B2 (en) Method of making a plastic colorimetric resonant biosensor device with liquid handling capabilities
US7663092B2 (en) Method and apparatus for phase contrast quadrature interferometric detection of an immunoassay
US7615339B2 (en) Method for producing a colorimetric resonant reflection biosensor on rigid surfaces
US20220357308A1 (en) Method and system for selecting chemical reagents in measurement of asphalt surface energy
JPH0755700A (ja) 光液浸液の屈折率を検査する装置および方法
Barrios et al. Molecularly imprinted polymer for label-free integrated optical waveguide bio (mimetic) sensors
US4254223A (en) Apparatus for colorimetric determination
WO2000008440A1 (de) Calibration device for an optical microtiter plate reading apparatus
JP2678463B2 (ja) 屈折率測定方法
EP1155305A2 (de) Handrefraktometer unm prüfen von bremsen
JPH028726A (ja) 屈折率測定方法
US6707542B1 (en) Refractometer for monitoring water content in fluids
CN115508311A (zh) 基于中空三棱柱透镜的透明溶液浓度测量装置及测量方法
US20230003722A1 (en) Methods and compositions for lateral flow analyte assays
CN102175646B (zh) 一种测量高折射率玻璃微珠折射率的装置及方法
JPH05332881A (ja) 光導波路断面屈折率分布測定装置
US2436567A (en) Microrefractometer for liquids
US8400551B2 (en) High resolution label free imaging
CN219224566U (zh) 一种透明溶液浓度测量装置
EP1892518A2 (de) Markierungsfreies optisches Verfahren mit hohem Durchsatz für den Nachweis biomolekularer Wechselwirkungen
CN108776380B (zh) 一种复合透镜和光谱仪

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AU CA ID JP KR US ZA

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)