WO2000005343A3 - Systeme de culture cellulaire destine a une transplantation neuronale - Google Patents

Systeme de culture cellulaire destine a une transplantation neuronale Download PDF

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Publication number
WO2000005343A3
WO2000005343A3 PCT/US1999/016825 US9916825W WO0005343A3 WO 2000005343 A3 WO2000005343 A3 WO 2000005343A3 US 9916825 W US9916825 W US 9916825W WO 0005343 A3 WO0005343 A3 WO 0005343A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
precursor cells
proliferating
differentiating
expanded
Prior art date
Application number
PCT/US1999/016825
Other languages
English (en)
Other versions
WO2000005343A2 (fr
Inventor
Lorenz Studer
Ron D G Mckay
Original Assignee
Us Health
Lorenz Studer
Ron D G Mckay
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health, Lorenz Studer, Ron D G Mckay filed Critical Us Health
Priority to AU51282/99A priority Critical patent/AU5128299A/en
Priority to US09/744,384 priority patent/US6787356B1/en
Publication of WO2000005343A2 publication Critical patent/WO2000005343A2/fr
Publication of WO2000005343A3 publication Critical patent/WO2000005343A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/815—Dopamine

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Neurology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Eyeglasses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de culture cellulaire qui comprend une étape de prolifération dans laquelle le nombre de cellules précurseurs est multiplié, et une étape de différenciation dans laquelle les cellules précurseurs multipliées sont transformées en cellules neuronales. L'étape de prolifération consiste à incuber les cellules précurseurs dans un milieu de prolifération contenant un facteur de croissance de fibroblastes basique (FGF basique). L'étape de différenciation consiste à incuber les cellules précurseurs dans des milieux de différenciation de manière à former un agrégat cellulaire n'adhérant sur aucune surface de la cuve d'incubation. Dans une forme de réalisation préférée, les cellules sont incubées dans un tambour tournant. Les milieux de différenciation peuvent également comprendre au moins un agent de différenciation. L'invention concerne en outre un procédé de traitement d'un trouble neurologique, tel que la maladie de Parkinson, un procédé permettant de transplanter un produit génique dans le cerveau d'un patient, un dosage destiné à des substances ayant une activité neurologique, et une culture cellulaire.
PCT/US1999/016825 1998-07-24 1999-07-23 Systeme de culture cellulaire destine a une transplantation neuronale WO2000005343A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU51282/99A AU5128299A (en) 1998-07-24 1999-07-23 Cell expansion system for use in neural transplantation
US09/744,384 US6787356B1 (en) 1998-07-24 1999-07-23 Cell expansion system for use in neural transplantation

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US9399198P 1998-07-24 1998-07-24
US60/093,991 1998-07-24
JP19604799A JP3291698B2 (ja) 1998-07-24 1999-07-09 可変眼鏡用レンズ
DE19954376A DE19954376A1 (de) 1998-07-24 1999-11-11 Verstellbare Fokussierlinse
FR9915140A FR2801986B1 (fr) 1998-07-24 1999-12-01 Lentille a distance focale variable pour verre de lunettes

Publications (2)

Publication Number Publication Date
WO2000005343A2 WO2000005343A2 (fr) 2000-02-03
WO2000005343A3 true WO2000005343A3 (fr) 2000-04-20

Family

ID=27438998

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/016825 WO2000005343A2 (fr) 1998-07-24 1999-07-23 Systeme de culture cellulaire destine a une transplantation neuronale

Country Status (5)

Country Link
JP (1) JP3291698B2 (fr)
AU (1) AU5128299A (fr)
DE (1) DE19954376A1 (fr)
FR (1) FR2801986B1 (fr)
WO (1) WO2000005343A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001076507A2 (fr) * 2000-04-11 2001-10-18 The University Of Miami Utilisation de transporteurs d'oxygene pour ameliorer la survie de cellules greffees dans une transplantation neuronale
AU2001259323A1 (en) * 2000-05-01 2001-11-12 Sang-Hun Lee Derivation of midbrain dopaminergic neurons from embryonic stem cells
US7250294B2 (en) * 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
FR2815042B1 (fr) * 2000-10-06 2005-01-07 Haguenauer Odile Cohen Utilisation d'une composition a activite antioxydante pour le conditionnement de cellules souches
EP1608728A2 (fr) * 2003-03-31 2005-12-28 University Of South Florida Research Foundation, Inc. Materiels et procedes pour reguler la formation de processus lors de la culture cellulaire
DE102007022452A1 (de) 2007-05-10 2008-11-13 Oerlikon Contraves Gmbh Modul, insbesondere Laser-/ Licht- Modul mit variabler Fokussierung

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009936A1 (fr) * 1989-12-26 1991-07-11 Hana Biologics, Inc. Produit cellulaire progeniteur neuronal proliferee et procede
WO1994010292A1 (fr) * 1992-10-28 1994-05-11 Neurospheres Ltd. Facteurs biologiques et cellules souches neurales
WO1994016718A1 (fr) * 1991-07-08 1994-08-04 Neurospheres Ltd. Modification genetique de cellules souches nerveuses
WO1996015224A1 (fr) * 1994-11-14 1996-05-23 Neurospheres Holdings Ltd. Induction in vitro de cellules dopaminergiques

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL83179A0 (en) * 1987-07-14 1987-12-31 Daniel Barnea Variable lens
US5668620A (en) * 1994-04-12 1997-09-16 Kurtin; Stephen Variable focal length lenses which have an arbitrarily shaped periphery
JPH08146205A (ja) * 1994-11-15 1996-06-07 Nippondenso Co Ltd 可変焦点レンズ
US5526067A (en) * 1994-12-21 1996-06-11 Polaroid Corporation Actuation mechanism for variable focal length eyeglasses
US5956183A (en) * 1998-05-26 1999-09-21 Epstein; Saul Field-customizable variable focal length lens

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009936A1 (fr) * 1989-12-26 1991-07-11 Hana Biologics, Inc. Produit cellulaire progeniteur neuronal proliferee et procede
WO1994016718A1 (fr) * 1991-07-08 1994-08-04 Neurospheres Ltd. Modification genetique de cellules souches nerveuses
WO1994010292A1 (fr) * 1992-10-28 1994-05-11 Neurospheres Ltd. Facteurs biologiques et cellules souches neurales
WO1996015224A1 (fr) * 1994-11-14 1996-05-23 Neurospheres Holdings Ltd. Induction in vitro de cellules dopaminergiques

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
STRECKER RE ET AL: "Survival and function of aggregate cultures of rat fetal dopamine neurons grafted in a rat model of Parkinson's disease", EXP BRAIN RES, vol. 76, no. 2, 1989, pages 315 - 22, XP000867471 *
STUDER L ET AL: "Transplantation of expanded mesencephalic precursors leads to recovery in parkinsonian rats [see comments].", NAT NEUROSCI, (1998 AUG) 1 (4) 290-5., XP000870304 *
STUDER, L. (1) ET AL: "Transplantation of in vitro expanded and reaggregated mesencephalic precursors leads to behavioral recovery in 60HDA lesioned rats.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, (1997) VOL. 23, NO. 1-2, PP. 1998. MEETING INFO.: 27TH ANNUAL MEETING OF THE SOCIETY FOR NEUROSCIENCE NEW ORLEANS, LOUISIANA, USA OCTOBER 25-30, 1997, XP000870279 *

Also Published As

Publication number Publication date
FR2801986B1 (fr) 2005-08-19
WO2000005343A2 (fr) 2000-02-03
FR2801986A1 (fr) 2001-06-08
JP2001042270A (ja) 2001-02-16
DE19954376A1 (de) 2001-05-17
AU5128299A (en) 2000-02-14
JP3291698B2 (ja) 2002-06-10

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