WO1999052625A2 - Procede et dispositif pour realiser des series de syntheses et d'analyses - Google Patents

Procede et dispositif pour realiser des series de syntheses et d'analyses Download PDF

Info

Publication number
WO1999052625A2
WO1999052625A2 PCT/DE1999/000957 DE9900957W WO9952625A2 WO 1999052625 A2 WO1999052625 A2 WO 1999052625A2 DE 9900957 W DE9900957 W DE 9900957W WO 9952625 A2 WO9952625 A2 WO 9952625A2
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
starting materials
products
accumulation
different
Prior art date
Application number
PCT/DE1999/000957
Other languages
German (de)
English (en)
Other versions
WO1999052625A3 (fr
Inventor
Hans Koch
Wolfhard Semmler
Original Assignee
Institut für Diagnostikforschung GmbH an der Freien Universität Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE1998117531 external-priority patent/DE19817531A1/de
Application filed by Institut für Diagnostikforschung GmbH an der Freien Universität Berlin filed Critical Institut für Diagnostikforschung GmbH an der Freien Universität Berlin
Priority to JP2000543230A priority Critical patent/JP2003522319A/ja
Priority to EP99924724A priority patent/EP1069941A2/fr
Publication of WO1999052625A2 publication Critical patent/WO1999052625A2/fr
Publication of WO1999052625A3 publication Critical patent/WO1999052625A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
    • B41J2/00—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed
    • B41J2/005—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by bringing liquid or particles selectively into contact with a printing material
    • B41J2/01—Ink jet
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
    • B41J3/00—Typewriters or selective printing or marking mechanisms characterised by the purpose for which they are constructed
    • B41J3/407—Typewriters or selective printing or marking mechanisms characterised by the purpose for which they are constructed for marking on special material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Definitions

  • the invention relates to a method for the production of synthesis or analysis series, in which the smallest variable volumes of different starting materials are combined to form a large number of different accumulation products and, if appropriate, examined with regard to certain physical and / or chemical properties, and to an apparatus for carrying out the method.
  • photometers are used for quantitative and qualitative analysis.
  • Samples of biological origin for example blood
  • a Reagent or several reagents mixed which react with the component to be detected in the sample. This reaction causes a specific change in the optical properties of the mixed product, which is detected with the photometer and recorded quantitatively.
  • the invention is therefore based on the object of specifying a method with which a multiplicity of precisely metered substance accumulations from two or more starting materials in different compositions and / or concentrations can be provided and, if appropriate, investigated in a very short time and with little outlay on equipment.
  • the object in a method for the production of synthesis and analysis series according to the
  • the preamble of claim 1 is solved in such a way that the accumulation products according to the inkjet printer principle are formed in a program-controlled manner at predetermined positions on an essentially planar substrate by the finest droplets or jets of the liquid, which can function as an exchangeable printhead and move linearly back and forth Material discharge head stored starting materials are applied to the surface of the substrate according to a specific computer program with regard to their respective dosage and positioning, and the accumulation products are subsequently analyzed in an analysis by a sensor connected to a linearly movable measuring head at the programmed positions, the substrate line by line a direction perpendicular to the linear movement of the material dispensing head or the measuring head is moved.
  • the basic idea of the invention lies in the use of an inkjet printer connected to a computer for producing a large number of synthetic products or mixtures of substances or reaction products which differ in their material and quantitative composition for a subsequent investigation with regard to certain properties or effects, the presence of active substances, etc .. That is, the known active principles of an inkjet printer trained for color printing are used, which in the program-controlled spraying of different substances and quantities of substance onto a base by controlled opening and closing of the respective nozzles of the material dispensing head connected to storage containers or electrostatic or magnetic deflection of the unwanted material beams exist to synthesize products or accumulation products for a subsequent analysis on a surface to produce formed substrate.
  • the material dispensing head can be made in one piece with several storage containers for different, separately controlled Nozzle channels can be formed, or a plurality of material dispensing heads, each with a storage container, can be provided in a depot, in which case the corresponding material dispensing head is activated in accordance with the material to be dispensed.
  • the material discharge head and the measuring head are arranged separately from each other. However, it is also conceivable to train both as one unit. ⁇ ⁇
  • the invention is used in the production of extensive synthesis series and analysis series, for example ELISA test with microtiter plates, in the creation of tests with combinatorial libraries or DNA chips, in cell differentiation, in the execution of tests in the field of dry chemistry and in vitro diagnostics or used in the photometric analysis of reaction kinetics.
  • both the individual material components and the substrate have a specific, coordinated surface tension in order to prevent the substances or the substance mixture from running on the substrate.
  • the substrate consists of a flexible material, such as paper or plastic film, or of a rigid base, for example made of glass, plastic or the like.
  • the substrate has a large number in a suitable manner, for example, lithographically produced depressions in the starting materials for the produced synthesis product or - that are then injected under investigation analysis product from the various reservoirs of the material dispensing head.
  • the accumulation products are generated in a protective gas atmosphere, in a vacuum or under a certain temperature and / or pressure regime.
  • the starting materials can also be heated themselves, if necessary, to bring them into a flowable state which can be easily discharged from the storage containers (material chambers) of the material dispensing head.
  • the substrate is prepared with certain substances in order to trigger an optical reaction for the analysis, for example.
  • a serum sample is to be examined for the presence of certain components, such as albumin, cholesterol, glucose and others.
  • certain components such as albumin, cholesterol, glucose and others.
  • the albumin present in a serum with bromocresol green should be detected.
  • the material chambers assigned to the print head acting as the material output head of a color jet printer ⁇ from each of which separate nozzle channels lead to the material output head, with the serum and with different reagents which, when mixed with the serum, are capable of mixing a specific enzyme into a certain color reaction with the serum to prove the serum, filled.
  • the material delivery head which can be moved back and forth on a rail, is connected to a computer. His movements too predetermined
  • the substrate used here is a transparent plastic film that, like in a printing process with an inkjet printer, is moved perpendicular to the direction of movement of the material dispensing head after a line on the substrate has been filled with the mixtures of sample and reagents.
  • the individual mixtures on the substrate which are now illuminated by a light source, are run over by the measuring head under program control, whereby a attached to this sensor Measures the color and intensity of the light emanating from the illuminated mixture.
  • the measurement results are forwarded to the computer and analyzed by it.
  • the application of the mixtures and their scanning of the sensor is carried out while measuring the air pressure, the air humidity and temperature, or under ⁇ ⁇ predefined external conditions, so that the examination is also reproducible. It is also conceivable that with the sequential supply of several samples to the sample chamber and with the use of intermediate washing processes, different serum samples are applied to the substrate film.
  • the method according to the invention is used to produce synthetic products from different amino acids.
  • a quartz plate with a large number of depressions formed by lithographic etching is used as the substrate.
  • the individual wells are controlled one after the other by the material dispensing head with its material chambers receiving the various starting substances, in order to use them in accordance with the ink jet printing process in the specified sequence and dosage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne un procédé pour produire une pluralité de différents produits d'accumulation de très faibles volumes à partir de différents produits de départ et avec des dosages variés des constituants individuels ainsi bien pour la synthèse que pour l'analyse des produits mélangés afin de déterminer leurs propriétés physiques et chimiques. Selon ce procédé, les produits d'accumulation sont appliqués en des positions prédéterminées d'un substrat plan, de façon commandée par programme, selon le principe de l'imprimante à jet d'encre. De minuscules gouttes des matériaux de départ coulants, stockés dans différents compartiments à matériaux associés à une tête de distribution de matériau, sont injectées, sur la base d'un programme informatique, dans des dosages déterminés, en des positions prédéterminées sur le substrat ou dans des évidements correspondants pratiqués dans ledit substrat, et éventuellement analysées au moyen d'un détecteur associé à une tête de mesure. Ce procédé permet, avec des appareils simples et peu nombreux, de façon très brève et avec des besoins réduits en matériaux, de préparer des produits d'accumulation dosés de manière précise, servant à réaliser des séries de synthèses et d'analyses.
PCT/DE1999/000957 1998-04-09 1999-03-25 Procede et dispositif pour realiser des series de syntheses et d'analyses WO1999052625A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2000543230A JP2003522319A (ja) 1998-04-09 1999-03-25 合成のため又は分析のための列を作成するための方法及び装置
EP99924724A EP1069941A2 (fr) 1998-04-09 1999-03-25 Procede et dispositif pour realiser des series de syntheses et d'analyses

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19817531.0 1998-04-09
DE1998117531 DE19817531A1 (de) 1998-04-09 1998-04-09 Verfahren und Vorrichtung zur Anfertigung von Synthese- oder Analysereihen
US9091998P 1998-06-29 1998-06-29
US60/090,919 1998-06-29

Publications (2)

Publication Number Publication Date
WO1999052625A2 true WO1999052625A2 (fr) 1999-10-21
WO1999052625A3 WO1999052625A3 (fr) 2000-01-06

Family

ID=26045628

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/000957 WO1999052625A2 (fr) 1998-04-09 1999-03-25 Procede et dispositif pour realiser des series de syntheses et d'analyses

Country Status (2)

Country Link
EP (1) EP1069941A2 (fr)
WO (1) WO1999052625A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000035940A2 (fr) * 1998-12-14 2000-06-22 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Procedes et dispositifs permettant d'appliquer des substances sur un substrat, notamment de monomeres pour la synthese combinatoire de bibliotheques de molecules
WO2002057015A2 (fr) * 2001-01-18 2002-07-25 Roland Zengerle Dispositif et procede de dosage de petites quantites de liquide
WO2002081088A2 (fr) * 2001-04-05 2002-10-17 Morphochem Aktiengesellschaft für kombinatorische Chemie Dispositif de distribution automatique de volumes microscopiques de liquides

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5508200A (en) * 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
US5643798A (en) * 1990-04-04 1997-07-01 The Rockefeller University Instrument and method for the sequencing of genome
WO1997032212A1 (fr) * 1996-03-01 1997-09-04 Beckman Instruments, Inc. Systeme d'essais multiples simultanes de fixation de ligands
WO1997043611A1 (fr) * 1996-05-16 1997-11-20 Affymetrix, Inc. Systemes et procedes de detection de produits marques
WO1997044134A1 (fr) * 1996-05-17 1997-11-27 Incyte Pharmaceuticals, Inc. Dispositif et procede de production de gouttelettes par jets
WO1997045730A1 (fr) * 1996-05-30 1997-12-04 Biodx Procedes et appareil a groupement miniaturise de cellules pour le criblage sur la base de cellules

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643798A (en) * 1990-04-04 1997-07-01 The Rockefeller University Instrument and method for the sequencing of genome
US5508200A (en) * 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
WO1997032212A1 (fr) * 1996-03-01 1997-09-04 Beckman Instruments, Inc. Systeme d'essais multiples simultanes de fixation de ligands
WO1997043611A1 (fr) * 1996-05-16 1997-11-20 Affymetrix, Inc. Systemes et procedes de detection de produits marques
WO1997044134A1 (fr) * 1996-05-17 1997-11-27 Incyte Pharmaceuticals, Inc. Dispositif et procede de production de gouttelettes par jets
WO1997045730A1 (fr) * 1996-05-30 1997-12-04 Biodx Procedes et appareil a groupement miniaturise de cellules pour le criblage sur la base de cellules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
A.SCHOBERT ET AL.: "Accurate High-Speed Liquid Handling of Very Small Biological Samples" BIOTECHNIQUES., Bd. 15, Nr. 2, 1993, Seiten 324-329, XP002121713 EATON PUBLISHING, NATICK., US ISSN: 0736-6205 *
LEMMO A V ET AL: "CHARACTERIZATION OF AN INKJET CHEMICAL MICRODISPENSER FOR COMBINATORIAL LIBRARY SYNTHESIS" ANALYTICAL CHEMISTRY, Bd. 69, Nr. 4, 15. Februar 1997 (1997-02-15), Seiten 543-551, XP000681609 ISSN: 0003-2700 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000035940A2 (fr) * 1998-12-14 2000-06-22 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Procedes et dispositifs permettant d'appliquer des substances sur un substrat, notamment de monomeres pour la synthese combinatoire de bibliotheques de molecules
WO2000035940A3 (fr) * 1998-12-14 2000-10-12 Deutsches Krebsforsch Procedes et dispositifs permettant d'appliquer des substances sur un substrat, notamment de monomeres pour la synthese combinatoire de bibliotheques de molecules
US9752985B2 (en) 1998-12-14 2017-09-05 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Method and devices for applying substances to a support, especially monomers for the combinatorial synthesis of molecule libraries
WO2002057015A2 (fr) * 2001-01-18 2002-07-25 Roland Zengerle Dispositif et procede de dosage de petites quantites de liquide
WO2002057015A3 (fr) * 2001-01-18 2002-12-05 Roland Zengerle Dispositif et procede de dosage de petites quantites de liquide
WO2002081088A2 (fr) * 2001-04-05 2002-10-17 Morphochem Aktiengesellschaft für kombinatorische Chemie Dispositif de distribution automatique de volumes microscopiques de liquides
WO2002081088A3 (fr) * 2001-04-05 2003-03-13 Morphochem Ag Komb Chemie Dispositif de distribution automatique de volumes microscopiques de liquides

Also Published As

Publication number Publication date
WO1999052625A3 (fr) 2000-01-06
EP1069941A2 (fr) 2001-01-24

Similar Documents

Publication Publication Date Title
EP0469444A1 (fr) Procédé et dispositif pour appliquer des doses de réactifs biochimiques sur un support
WO1998042442A1 (fr) Microplaque a fond transparent
DE10013242A1 (de) Chemisches Analysegerät und chemisches Analysesystem
DE19628928A1 (de) Feste Träger für analytische Meßverfahren, ein Verfahren zu ihrer Herstellung sowie ihre Verwendung
DE2641097A1 (de) Vorrichtung zur probenahme, zum mischen der probe mit einem reagens und zum durchfuehren von insbesondere optischen analysen
DE3403978C2 (fr)
DE2650505A1 (de) Verfahren und vorrichtung zum bestimmen der reaktionszeit
WO1999064158A1 (fr) Support de reacteur a plusieurs chambres de reception de microechantillons
EP0905515B1 (fr) Méthode pour la mésure analytique et son usage
EP1610129A2 (fr) Procédé et dispositif de préparation de substrates avec affinité pour un analyte
WO2018077352A1 (fr) Dispositif et procédé de traitement d'échantillons contenant des cellules biologiques
EP2427267B1 (fr) Support reconnaissable pour procédés de mesure optiques
WO2020010379A1 (fr) Procédé et dispositif pour l'exécution de dosages immunologiques hétérogènes
WO2003056335A2 (fr) Reseau de micropuits permettant de trier ou separer des cellules en suspension dans un fluide en ecoulement en fonction de leur taille et procede pour analyser l'activite fonctionnelle de cellules individuelles
WO1999052625A2 (fr) Procede et dispositif pour realiser des series de syntheses et d'analyses
DE19817531A1 (de) Verfahren und Vorrichtung zur Anfertigung von Synthese- oder Analysereihen
EP1286771B1 (fr) Chambre de microhybridation
DE4120139A1 (de) Enzymimmunoassay-verfahren und testset hierzu
EP1397201B2 (fr) Recipient de reaction servant a produire des echantillons
DE2341158C3 (fr)
AT500427B1 (de) Vorrichtung zur analyse von bestandteilen einer probe
EP1594613B1 (fr) Procede d'analyse d'echantillons cellulaires
DE10246446B4 (de) Verfahren zum Aufbringen eines Probenfilms auf einen Probenträger
AT520151B1 (de) Analysator und verfahren zur durchführung von chemischen, biochemischen und/oder immunchemischen analysen
EP1116035A1 (fr) Dispositif permettant de mesurer la capacite migratoire des cellules a deplacement amiboide

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CN IL JP KR US ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): CN IL JP KR US ZA

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

WWE Wipo information: entry into national phase

Ref document number: 1999924724

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 09623207

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999924724

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999924724

Country of ref document: EP