WO1999010358A3 - Verfahren zum herstellen von nukleinsäurepolymeren - Google Patents

Verfahren zum herstellen von nukleinsäurepolymeren Download PDF

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Publication number
WO1999010358A3
WO1999010358A3 PCT/EP1998/005219 EP9805219W WO9910358A3 WO 1999010358 A3 WO1999010358 A3 WO 1999010358A3 EP 9805219 W EP9805219 W EP 9805219W WO 9910358 A3 WO9910358 A3 WO 9910358A3
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WO
WIPO (PCT)
Prior art keywords
cross
linkable
oligonucleotides
nucleic acid
synthesis
Prior art date
Application number
PCT/EP1998/005219
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English (en)
French (fr)
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WO1999010358A2 (de
Inventor
Peter Hegemann
Original Assignee
Peter Hegemann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peter Hegemann filed Critical Peter Hegemann
Priority to EP98947434A priority Critical patent/EP1005574A2/de
Priority to DE29823795U priority patent/DE29823795U1/de
Priority to US09/486,241 priority patent/US6472184B1/en
Publication of WO1999010358A2 publication Critical patent/WO1999010358A2/de
Publication of WO1999010358A3 publication Critical patent/WO1999010358A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

Die herkömmliche Synthese von Nukleinsäurepolymeren aus mehreren Oligonukleotiden umfaßt mehrere Zyklen der Zwischenproduktsynthese, Reinigung der Zwischenprodukte und Synthese eines Vollängenproduktes (bis zu einer Maximallänge von ca. 1000 Nukleotiden). Das neue Verfahren soll in einem Reaktionsschritt durchgeführt werden und zu Nukleinsäurepolymeren von mehr als 1000 Nukleotiden führen. Erfindungsgemäß werden zwei oder mehr verknüpfbare Oligonukleotide bereitgestellt, die bei kontinuierlicher Anordnung und nach Verknüpfen einen Primärstrang bilden können, und ein oder mehr nicht verknüpfbare Oligonukleotide, wobei jedes der nicht verknüpfbaren Oligonukleotide zwei aneinandergrenzende Bereiche umfaßt, deren erster dem 3'-Ende eines verknüpfbaren Oligonukleotides komplementär ist und deren zweiter am 5'-Ende eines weiteren verknüpfbaren Oligonukleotides komplementär ist. Die verknüpfbaren Oligonukleotide werden mit den komplementären Bereichen der nicht verknüpfbaren Oligonukleotide hybridisiert und anschließend enzymatisch, chemisch oder photochemisch verknüpft. Das Verfahren eignet sich zur de novo-Synthese langer Nukleinsäureketten.
PCT/EP1998/005219 1997-08-22 1998-08-17 Verfahren zum herstellen von nukleinsäurepolymeren WO1999010358A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP98947434A EP1005574A2 (de) 1997-08-22 1998-08-17 Verfahren zum herstellen von nukleinsäurepolymeren
DE29823795U DE29823795U1 (de) 1997-08-22 1998-08-17 Oligonukleotid zur Verwendung der Synthese eines modifizierten Genes für GEP
US09/486,241 US6472184B1 (en) 1997-08-22 1998-08-17 Method for producing nucleic acid polymers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19736591.4 1997-08-22
DE19736591A DE19736591A1 (de) 1997-08-22 1997-08-22 Verfahren zum Herstellen von Nukleinsäurepolymeren

Publications (2)

Publication Number Publication Date
WO1999010358A2 WO1999010358A2 (de) 1999-03-04
WO1999010358A3 true WO1999010358A3 (de) 1999-08-05

Family

ID=7839861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/005219 WO1999010358A2 (de) 1997-08-22 1998-08-17 Verfahren zum herstellen von nukleinsäurepolymeren

Country Status (4)

Country Link
US (1) US6472184B1 (de)
EP (1) EP1005574A2 (de)
DE (2) DE19736591A1 (de)
WO (1) WO1999010358A2 (de)

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Citations (5)

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WO1994012632A1 (en) * 1992-11-27 1994-06-09 University College London Improvements in nucleic acid synthesis by pcr
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Also Published As

Publication number Publication date
WO1999010358A2 (de) 1999-03-04
DE19736591A1 (de) 1999-02-25
EP1005574A2 (de) 2000-06-07
DE29823795U1 (de) 2000-03-02
US6472184B1 (en) 2002-10-29

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