WO1999009808A1

WO1999009808A1 - Spawning apparatus

Info

Publication number: WO1999009808A1
Application number: PCT/JP1998/002846
Authority: WO
Inventors: Masao Kondo; Yuichi Maruyama; Tomoyuki Ogake; Masahiko Hujisawa
Original assignee: Nippon Seiki Co., Ltd.
Priority date: 1997-08-22
Filing date: 1998-06-24
Publication date: 1999-03-04
Also published as: KR20000068790A; CN1126439C; KR100343648B1; CN1236297A

Abstract

A spawning apparatus (11) comprising a main body frame (1), a spawn container (10) disposed in the main body frame (1) with the mouth portion thereof disposed upside down and a spawn scraping mechanism (32) for scraping out a predetermined amount of spawns (S) such as fungi contained in the spawn container (10), wherein the spawns (S) scraped out by the spawn scraping mechanism (32) are supplied into a culture container or a culture package bag containing a culture medium. A device (B) for pre-treating the spawn container (10) is provided before the spawn scraping mechanism (32).

Description

Specification

Technical field

TECHNICAL FIELD The present invention relates to an inoculum inoculation device for inoculating a culture medium filled in a cultivation container or a cultivation packaging bag with a mushroom inoculum. Background art

Conventionally, as a method of artificially cultivating mushrooms using a culture medium containing a sawdust or rice bran as a main component and a nutrient source, a culture medium is filled into a cultivation container, sterilized, and the enoki mushroom is placed in the filled cultivation container. After inoculating the seeds of mushrooms such as mushrooms and mushrooms, cover the mouth of the cultivation container with a lid, close the cultivation container, and cultivate the bacterium under predetermined temperature and humidity conditions. Is filled in a plastic packaging bag, the bag opening of the filled culture medium-filled packaging bag is folded back and closed, and the culture medium together with the bag is sterilized in this state. There is a method of inoculating a seed bacterium inside the bacterium and cultivating the bacterium under predetermined temperature and humidity conditions for industrial cultivation.

By the way, as a preparatory step before inoculating the seed medium into the culture medium in the conventional cultivation container or cultivation packaging bag, the surface of hardened and aged seed bacteria in the seed container is removed, There is removal work.

In addition, the inoculation of the inoculum into the cultivation container or cultivation bag during the inoculation of the inoculum by the inoculation device, which is the next step, is to prevent the inoculum from adhering to the inside of the inoculum container or the mouth side of the container. Sterilization is performed in the pretreatment stage of the inoculation device.

Conventionally, in the pre-inoculation process of the inoculum using the inoculum inoculation device, the above-described inoculation and removal of the inoculum is performed manually using an extraction rod for extracting the inoculum. is the current situation.

Further, at this time, in order to prevent invasion of various bacteria, the tip of the extraction rod is dipped in an alcohol and flame-sterilized on an alcohol lamp. To remove only the surface of the hardened inoculum in the inoculum container and remove the inoculum on the surface from the inoculum container. In addition, the mouth of the inoculum container may also be sterilized by spraying with alcohol, wiping with alcohol, or sterilizing by heating.

By the way, pre-treatments such as the removal and sterilization of the inoculum by manual labor are manual operations, and therefore require a lot of time, and are also required to be manually extracted by an extraction rod. In order to determine the depth of the seed container from the mouth of the seed container, there is a variation in the depth of the seed container. In the next step, there is a risk of invasion of various bacteria into the inoculum container. The inoculated inoculum container had to be set in a state where it was kept from invading various bacteria, which required much time and effort. In addition, there was a variation in the sterilization state, and there was a problem in management.

Therefore, the present invention automatically performs the pretreatment of the inoculum, which is performed as a preparatory step for inoculation of the inoculum to be supplied into the cultivation container or the cultivation bag, and further sequentially transmits the inoculum container after the pretreatment to the inoculum of the inoculum inoculation apparatus.を It is an object of the present invention to provide an inoculum inoculation device that can be supplied to an ejection mechanism position. Disclosure of the invention

According to the present invention, there is provided a seed frame exposing mechanism for disposing a seed cell such that mushrooms or the like contained in the seed cell container are disposed on the main body frame with the mouth side of the seed cell container turned upside down, A seed inoculation device configured to supply a seed bacterium extracted by the seed germ ejection mechanism into a cultivation container or a cultivation packaging bag filled with a culture medium, wherein the seed germ ejection mechanism is provided. In the pre-process, a pretreatment device for pretreating the inoculum container was installed, so that the inoculum container is automatically pretreated by this pretreatment device, and the inoculum container that has completed the pretreatment is ejected by the inoculum ejection mechanism. Should be supplied to

Further, the pretreatment device comprises an inoculum surface removing and removing mechanism having a fungal removal blade for removing a surface portion of the inoculum in the inoculum container. The surface of the inoculum contained in the inoculum container It is sufficient to supply the inoculum container that has been subjected to the pretreatment to the inoculum ejection mechanism.

In addition, by providing a sterilization treatment apparatus for sterilizing the seeding blade of the seed removal and removal mechanism, a surface part of the hardened and aged seed in the seed container is removed before and after the removal. After the wiping, the wiping blade of the inoculum surface wiping and removing mechanism is sterilized by the germicidal treatment device to prevent invasion of various germs into the inoculum container.

Further, the pretreatment device is constituted by a sterilization treatment device for sterilizing the mouth of the seed culture container, so that the mouth of the seed culture container is sterilized by the sterilization treatment device, thereby preventing invasion of various bacteria into the seed culture container. Is done.

In addition, the pretreatment device includes a seed-bacteria surface-peeling and removing mechanism having a fungus cutting blade that scrapes a surface portion of the aged seed-bacteria in the seed-bacteria container, and sterilizes the mouth of the seed-bacteria container. The sterilization apparatus comprises a sterilization apparatus, and the sterilization apparatus simultaneously sterilizes the bacteria cutting blades of the inoculum removing and removing mechanism. Even if the inoculated and removed inoculum comes into contact with the mouth of the inoculum container and the germs adhere to it, the inoculum of the inoculum container is sterilized by the sterilization treatment device, which is a heating means consisting of the hot air generator, so that it can be sterilized in the subsequent process. It can prevent inoculation of inoculum with various germs during inoculation of the inoculum.

Further, by providing the seed container rotating mechanism for rotating the seed container, the entire periphery of the mouth portion of the seed container is heat-sterilized, so that the inoculum with various germs is inoculated during the inoculation of the inoculum performed in a later step. It can be prevented.

In addition, since the sterilization treatment device includes heating means, the sterilization treatment device, which is the heating means, heat-sterilizes the mouth of the seed culture container and / or the sterilization blade of the seed culture removal and removal mechanism. It can prevent inoculation of inoculum with various germs at the time of inoculation of inoculum performed in the above.

A seed cell container mounting member for disposing the seed cell container in an inverted state; a seed cell ejecting mechanism for discharging a predetermined amount of seed cells such as mushrooms contained in the seed cell container mounted on the seed cell container mounted on the seed cell container mounting member. A guide member for intermittently supplying the inoculum container mounting member to the inoculum ejection mechanism sequentially; and providing the inoculum container mounting member connected to the guide member. Sequentially at the inoculum extraction device position A seed inoculation device that supplies and extracts a predetermined amount of seed and supplies it to a cultivation container or a cultivation packaging bag is provided so as to protrude laterally from the guide member in a direction orthogonal to a transfer direction by the guide member. By providing a pretreatment device for pretreating the seed container, and a seed inoculation pretreatment transport mechanism that reciprocates the seed container mounting member between the guide member and the position of the pretreatment device, the seed container is provided. Is set in the inoculum container mounting member, so that the inoculum container is placed in a predetermined pretreatment device by the inoculum pretreatment transport mechanism by the inoculum pretreatment transport mechanism at the preprocess position of the inoculum extraction mechanism of the inoculum inoculation device. The pre-treatment is carried out, and after the pre-treatment is completed, the pre-treatment is returned to the guide member and conveyed. It can be sequentially fed into the feeding apparatus position can bacteria 搔.

In addition, the inoculation container loading member can pre-process the inoculum containers with a small number of pretreatment devices by arranging the inoculum containers in a horizontal row, thereby making it possible to reduce the manufacturing cost.

Further, in the guide member of the inoculum inoculation device, the inoculum container mounting member in a standby state at a pre-process position of inoculum inoculation by the inoculum ejection device is transferred to the pretreatment device by the inoculum inoculation pretreatment transport mechanism. Since it is configured to transport and return to the guide member after performing a predetermined pretreatment, it is necessary to perform the pretreatment work while extracting the inoculum by the inoculum ejection mechanism and inoculating the inoculum. Thus, the inoculum inoculation step and the inoculum pretreatment step can be performed simultaneously without affecting the inoculum inoculation step. BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a schematic perspective view showing an overall configuration of a seed inoculum inoculation device applied in one embodiment of the present invention, and FIG. 2 is a main side of the inoculum inoculation device applied in one embodiment of the present invention. FIG. 3 is a partial perspective view showing a main part of the inoculum inoculation device in the inoculum inoculation device applied in one embodiment of the present invention, and FIG. 4 is an embodiment of the invention. Fig. 5 is a front view of a main part of the inoculum inoculation apparatus applied in the example, showing a state when the seed container is released; Fig. 5 is a front view of the main part showing a state when the seed container in Fig. 4 is set; FIG. 6 is a schematic diagram showing a configuration of a pretreatment step of the inoculum inoculation apparatus applied to one embodiment of the present invention, and FIG. 7 is a front view of the inoculum inoculation apparatus of FIG. Seed extract removal machine FIG. 8 is a main side view mainly showing the structure, and FIG. 8 is a main side view showing the inoculum extraction and removal mechanism and the sterilizing apparatus shown in FIG. 7, and FIG. 9 is a sterilizing apparatus shown in FIG. FIG. 10 is a structural diagram of a hot-air generator as a processing device. FIG. 10 is an operation explanatory diagram of a pretreatment step in the inoculum inoculation device applied to one embodiment of the present invention. FIG. It is operation | movement explanatory drawing of the pretreatment process in the inoculum inoculation apparatus applied to one Example of this invention, FIG. 12 is operation | movement explanatory drawing of the pretreatment process in the inoculum inoculation equipment applied to one Example of this invention. FIG. 13 is an explanatory diagram of an operation of a pretreatment step in the inoculum inoculation apparatus applied to one embodiment of the present invention, and FIG. 14 is an application diagram of one embodiment of the present invention. It is operation | movement explanatory drawing of the pre-processing process in an inoculum inoculation apparatus. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. The schematic configuration and operation of the inoculum inoculation device according to the present invention will be described with reference to the schematic diagrams showing the overall configuration of the inoculum inoculation device shown in FIGS. 1 to 5. In the present embodiment, an example is described in which a pretreatment device including a seed removal device and a sterilization treatment device is provided at a location before the seed inoculation step.

In the inoculum inoculation apparatus 11 to which the present invention is applied, a transfer conveyor 2 is provided over substantially the entire length of the main body frame 1, and the transfer conveyor 2 is started and stopped by operating a switch on the operation panel 3. The endless chain 4 is extended in parallel between a plurality of sprockets provided on both ends of the main body frame 1. Then, a cultivation container 6 filled with a culture medium mainly containing sawdust and rice bran and containing a nutrient source and sealed with a lid 5 is transferred, for example, a plastic container 7 containing four columns and four rows, and the transfer start side. The container 7 is placed on the chain 4 of the transfer conveyor 2 in the above, and when the transfer conveyor 2 is started, the container 4 can be conveyed toward the transfer end by the movement of the chain 4.

Also, as shown in Japanese Patent Application Laid-Open No. 5-103543, proposed by the applicant of the present invention, the cultivation vessels 6 are arranged in a plurality of rows at a substantially central position of the main body frame 1. The stored containers 7 are transferred from the loading side to the inoculum inoculation position, and at the inoculum inoculation position, the conveyors are transferred in a row of the cultivation containers 6 in the transfer direction of the container 7. In this case, the transfer conveyor 2 is stopped intermittently when it is detected that the cultivation container 6 has reached an appropriate inoculum inoculation position, and the container 7 is detected. A container positioning device 8 is provided to position the cultivation containers 6 in a row in a row, and the lids of the cultivation containers 6 are positioned and arranged in a row in the seed inoculation position by the container positioning device 8. There is provided a lid opening / closing device 9 for opening and closing 5.

When the lid 5 of the cultivation container 6 is opened by the lid opening / closing device 9, the cultivation container 6 is located above the lid opening / closing device 9, and is opened by exposing a predetermined amount of the inoculum S in the inoculum container 10. The inoculum inoculation device 1 inoculates 1.

The inoculum inoculation device 11 configured above the main body frame 1 is disclosed in, for example, Japanese Patent Application Laid-Open No. H08-126463, which was previously proposed by the present applicant. In the inoculum inoculation device 11, the mouth portions 10 A of the four inoculum containers 10 corresponding to the number of the horizontal cultivation containers 6 accommodated in the container 7 are inserted in an inverted state. A seed container mounting member 13 comprising a substantially flat loading plate provided with mounting holes 12 to be provided is provided. The seed container mounting member 13 is a rail-shaped guide mounted on both sides of the main body frame 1. It is placed on member 14.

In this embodiment, the guide member 14 is configured such that both end portions of the inoculum container mounting member 13 can be mounted thereon, and that the guide member 14 is inclined at a predetermined angle so as to be slidable by the weight of the inoculum container mounting member 13. 14 is attached and fixed to the main body frame 1, and a stopper portion 14 A for defining a sliding movement area of the inoculum container mounting member 13 is provided at the lowermost end of the guide member 14.

Further, on both sides of the main body frame 1 corresponding to the extraction position where the inoculum S of the inoculum container 10 is ejected, plate-like supports 15 are respectively provided upright along the inclination angle of the guide member 14. Air cylinders 16 that support or release the inoculum container 10 are attached to the columns 15 arranged at the predetermined angle and the pistons of the air cylinders 16 are installed. A support member 17 is bridged and fixed between the upper ends, and the support member 17 is disposed in an upper space of the guide member 14 so as to be inclined in parallel with the guide member 14.

Further, the support member 17 is provided at a position corresponding to the bottom 10 B of the inoculum container 10. A plurality of sprockets 18 each serving as a rotation transmitting member are rotatably supported via a rotating shaft 19, and the lower end of each rotating shaft 19 has a bottom portion 10 B of a seed container 10. A disk-shaped pressing plate 20 of approximately the same size is attached to and fixed to the rotating shaft 19, and the support member 17, a rotating shaft 19 attached to the supporting member 17, and a pressing plate A pressing support member 21 that presses the bottom 10 B of the seed container 10 by 20 or the like is formed. In this case, the disc-shaped pressing plate 20 that forms a part of the pressing support member 21 is formed. Is configured to be detented with respect to the rotation shaft 19 and to press and urge the bottom portion 10B of the inoculum container 10 sporadically.

Further, a motor 22 provided with a gear head is mounted and fixed to the support member 17 facing the sprocket 18 side, and a driving sprocket 23 is mounted on the rotating shaft of the motor 22. An endless shape is provided between the driving sprocket 23 and the sprocket 18 serving as the rotation transmitting member for rotating the pressing plates 20 and the driven sprocket 24 provided on the supporting member 17. And the sprockets 18, 23, 24 and the roller chain 25 constitute a seed bacterium container rotation drive member 26, and the motor 22. The rotation of each sprocket 18 is transmitted via an endless roller chain 25 by rotation of the sprocket 18, and the pressing plate 20 fixed to each sprocket 18 is rotated via a rotating shaft 19. Have been .

In addition, at the extraction position where the inoculum S of the inoculum container 10 is ejected, the support member 17 constituting a part of the pressing support member 21 is provided with the inoculum container mounting member 13 at both ends. Positioning shafts 27 project in pairs to the left and right, and fitting holes 28 are formed at both ends of the inoculum container mounting member 13 opposed to the tip of the positioning shaft 27, respectively. The positioning shaft 27 and the fitting hole 28 constitute a position regulating member 29 for fixing and supporting the inoculum container mounting member 13 on the guide member 14.

Further, in the inoculum inoculation apparatus according to the above-described embodiment, when the inoculum container 10 is loaded and held in the inoculum container mounting member 13, the inoculum container 10 filled with the inoculum S is turned upside down. The mouth part 1 OA of the seed container 10 is mounted on the four mounting holes 12 of the seed container mounting member 13 formed on the side, and set in a horizontal line. On the other hand, as shown in FIG. The cylinder 16 is operated to maintain the piston of the air cylinder 16 at the raised position. With the rise of the piston, the support member 17 and the pressing plate 20 attached to the support member 17 and the support member 17 are provided. The positioning shaft 27 of the position regulating member 29 attached to the container is kept in the raised state, and in this raised state, the seed container mounting member 13 previously set to the state where the seed container 10 is inverted is used to expose the seed S. The support member 17 fixed to the piston is moved down by moving the piston of the air cylinder 16 down by placing it on the guide member 14 located below the pressing plate 20 side, which is the position. As a result of this lowering operation, as shown in FIG. 5, the pressing plate 20 attached to the support member 17 and the positioning shaft 27 of the position regulating member 29 descend, and the pressing plate 20 Therefore, the bottom portion 10B of each inoculum container 10 is pressed and supported, and the tip of the positioning shaft 27 constitutes a part of a position regulating member 29 provided on the plate-shaped inoculum container mounting member 13. The seed-stain container mounting member 13 slidably mounted on the guide member 14 is inserted into the fitting hole 28 of the positioning member 27 and the positioning shaft 27, which is the position regulating member 29, and the fitting hole 28. Then, the seed is fixed and supported at a predetermined position by the insertion, and the preliminary preparation in the step of extracting the inoculum S in the inoculum container 10 is completed.

In addition, as described above, the seed cell container mounting member 13 in this embodiment includes various cell containers 10 in accordance with the number (four) of the row units of the cultivation containers 6 accommodated in the container 7. In this case, four mounting holes 12 for receiving the mouth 1 OA are formed, and support shafts 30 are erected between the mounting holes 12, respectively. At the upper end, a substantially cylindrical piece 31 supporting the body 10 C of the seed container 10 is fixedly attached, and the body of each seed container 10 arranged in an inverted state by the piece 31 is fixed. The section 10C is supported so that it does not fall down.

Further, the inoculum inoculation device 11 is provided with an inoculum ejection mechanism 32 for extruding the inoculum S in the inoculum container 10 through the mounting holes 12 of the inoculum container mounting member 13. The inoculation mechanism 32 is formed by an ejection shaft 34 provided with an ejection blade 33 at the tip thereof. The rotation transmission of the motor 35, which is a member, is transmitted via the chain 36. In addition, the motor shaft 35 and the output shaft 34 are mounted on the plate 37 of the body frame 1.

In addition, as shown in Japanese Patent Application Laid-Open No. 5-103543, which was previously proposed by the present applicant, an ejection shaft 34 provided with the ejection blade 33 is provided. The plate 37, which supports each extraction shaft 34, is provided so as to be able to move up and down together with the rotation of the extraction blade 33. In this embodiment, the female screw cylinder 38 provided on the plate 37 is provided. A screw shaft 39 is screwed into the motor shaft 41, and the screw shaft 39 is connected to a motor 41 serving as an ascending drive member through an endless chain 40. Is transmitted to the screw shaft 39 via the chain 40, and the plate 37 is raised with the rotation of the screw shaft 39, and the ejection mounted on the plate 37. The extraction shaft 34 provided with the blade 33 is guided to ascend.

The motor 41 serving as the protrusion raising drive member rotates in reverse so that the protrusion shaft 34 returns to the lower end when the protrusion shaft 34 reaches the rising end. Thus, the plate 37 is configured to return downward.

In addition, below the four mounting holes 12 provided in the inoculum container mounting member 13, there are provided inoculum hoppers 42 for guiding and storing the inoculum S in correspondence with the respective inoculum hoppers 4 2. The lower end is provided with a shutter 43 for opening and closing the lower end of the opening.

Further below the shutter 43, a seed supply tube 44 for guiding the inoculum S to the mouth of the cultivation container 6 when the shirt 43 is opened is disposed. 4 and 5 are provided so as to be movable together with the lid opening / closing device 9 via a link arm, and the inoculum is interlocked when the lid 5 is closed by the lid opening / closing device 9 due to the operation of the cylinder 45. The supply cylinder 44 is movably disposed so as to retreat to a position away from the cultivation container 6.

Further, in the inoculum inoculation device to which the present invention is applied, a predetermined number of the inoculum container mounting members 13 can be placed on the upper side of the main body frame 1, that is, on the side where the guide member 14 is carried in, A seed container supply mechanism 46 is provided, which enables the container mounting member 13 to be supplied intermittently sequentially.

The inoculum container supply mechanism 46 is located on the upper flat surface of the An endless chain 48 is hung in parallel between the sprockets 47 provided on the end side, and a motor 49 serving as a loading / driving drive means is connected to one of the sprockets 47 via a gear. The inoculum container mounting member 13 placed on the endless chain 48 is intermittently fed by the rotational drive of the motor 149.

On both sides of the endless chain 48 spanned between the sprockets 47, guide side plates 50 for guiding the inoculum container mounting member 13 are provided, and the endless chain 48 is carried out. The guide member 14 is disposed adjacent to the side of the chain at a predetermined angle, and the guide piece 51 is attached and fixed to the guide side plate 50 located at the carry-out end of the chain 48. When the inoculum container mounting member 13 is shifted by the guide piece 51 from the chain 48 to the guide member 14 arranged obliquely, the rear end side of the inoculum container mounting member 13 is a guide member 14. They are trying to prevent them from floating upside down.

In addition, on the unloading side of the guide member 14, a seed container unloading mechanism 52 to which the seed container mounting member 13 is delivered is installed, and the seed container unloading mechanism 52 is entirely frame-shaped. An unloading rail 52A is arranged on both sides in accordance with the width of the rail-shaped guide member 14, and a drop-preventing stopper is formed at the unloading end of the unloading rail 52A. ing.

The stop part 14A described above is connected to a cylinder 53 serving as a stopper releasing means so as to be able to protrude and retract with respect to the conveying surface of the rail-shaped guide member 14, and this cylinder is connected to the cylinder part 14A. When the stopper 14 A is retracted by the operation of 5 3, the inoculum container mounting member 13 in which the inoculum 10 on which the inoculum S on the rail-shaped guide member 14 is empty is loaded is inclined. It slides along the surface and is transferred to the unloading rail 52 A, which is the inoculum container unloading mechanism 52, and is formed so that the inoculum container mounting member 13 is positioned and stopped by the stopper for preventing fall. Have been. By the way, in the present invention, as shown in FIGS. 6 to 9 in addition to the above-mentioned FIG. 2, the inoculation device 11 is provided with an inoculation device 32 and an inoculation container supply mechanism 46. That is, in the preceding step of the step of extracting the inoculum S by the inoculum injecting device 11 of the incubator 11, the aged inoculum S in the inoculated inoculum container 10 in the process preceding the extraction step of the inoculum S

0 Seed surface removal and removal mechanism 54 that removes the surface of the seeds, and some of the components of the seed surface removal and removal mechanism 54 A pretreatment device B comprising a sterilization treatment device 55 comprising means is provided on the main body frame 1 of the inoculum inoculation device 11.

In other words, these pretreatment devices B are disposed so as to protrude laterally from the guide member 14 in a direction perpendicular to the direction of transport of the seed cell container mounting member 13 by the guide member 14.

Then, the seed container mounting member 13 fed from the seed container supply mechanism 46 is stocked in the middle of the rail-shaped guide member 14, and the seed container mounting member 13 is transferred to the pretreatment device B described above together with the seed container mounting member 13. A seed inoculation pretreatment transport mechanism 56 for feeding the seed container 10 is configured.

The inoculum inoculation pretreatment transport mechanism 56 has a frame-like shape provided with a pretreatment step stopper for stopping the inoculum container mounting member 13 sent out from the inoculum container supply mechanism 46 at a predetermined position. The formed mounting slide member 57 is provided, and the frame-shaped mounting slide member 57 can reciprocate with the slider section 60 via a guide rail 59 mounted on the fixed frame 58 side. Is provided. By the seed inoculation pretreatment transport mechanism 56, the seed bacteria container mounting member 13 reciprocates between the guide member 14 and the preprocessing device B position.

In this embodiment, as a reciprocating mechanism of the placing slide member 57, the driving source is constituted by a motor 61, and the rotational driving of the motor 61 is performed via a pinion and a rack (not shown). Although the placing slide member 57 is configured to reciprocate, it may be configured by a driving means such as a cylinder.

Further, in the reciprocating movement area of the placing slide member 57, a pretreatment device B including the inoculum surface removal and removal mechanism 54 and the sterilization treatment device 55 is provided.

In addition, as a pretreatment step as a pre-step of inoculating the culture medium in the cultivation container 6 or a cultivation packaging bag (not shown) with the inoculum S, the surface of the hardened and aged inoculum S in the inoculum container 10 is opened. The inoculum surface removing and removing mechanism 54 for removing the inoculated bacteria is provided with a female screw bearing portion 63 on a fixed panel 62 attached to the main frame 1 of the inoculum inoculation device 111. Screw the screw shaft 6 4 A bacterium for extracting only the surface portion of the aged inoculum S in the inoculum container 10 through the mounting holes 12 of the inoculum container mounting member 13 at the tip of the ejection screw shaft 64. A blade 65 is provided, and a motor 66 is connected to a lower end portion of the germ extraction screw shaft 64 for guiding the rotation of the germ extraction blade 65 while rotating.

In addition, the motor 66 is fixed to a motor mounting plate 67, and a slider portion 68 is mounted on the motor mounting plate 67, and the slider portion 68 is mounted on the fixed panel 62. It is mounted so as to be able to move up and down along the mounted guide rails 69.

Accordingly, the rotation of the motor 66 drives the bacteria contacting screw shaft 64 to rotate, and the bacteria extracting screw shaft 64 pivotally supported by the female screw bearing 63 rises with the rotation. Along the guide rail section 69 attached to the fixed panel 62, the motor 66 attached to the fixed panel 62 is also guided upward via the slider section 68.

In addition, the bacteria contacting screw shaft 64 provided with the bacteria removal blade 65 on the upper end side, when the bacteria removal blade 65 reaches the predetermined depth dimension from the mouth 1 OA of the seed container 10, that is, The motor 66 is rotated in reverse so as to return to the descending end when it reaches the rising end, which has a depth dimension that removes only the surface portion of the hardened and aged seed bacteria S in the seed container 10. By this reverse rotation, the bacteria removal blade 65 and the motor 66 together with the bacteria contact screw shaft 64 are configured to return downward.

Further, in this embodiment, a sterilization treatment apparatus 55 including a heating means is configured to perform sterilization treatment near the mouth 1 OA of the inoculum container 10. The sterilizing apparatus 55 comprising this heating means is constituted by a hot air generator, and the hot air generator has a transparent quartz tube 71 in which a heating element 72 is housed, and The connecting pipe 73 provided on the rear end side is attached to the frame 1 side of the inoculum inoculation device, and a flexible hose 74 is connected to the rear end of the connecting pipe 73. An air supply source (not shown) supplies an air at a predetermined pressure to the supply port side of the air supply unit. As a result, the hot air blown out from the tip of the quartz tube 71 incorporating the light-absorbing element 72 toward the mouth 1 OA of the inoculum container 10 It is blown out and heated.

In addition, the seed container container rotates by rotating the seed container 10 by pressing the bottom 10 B of the seed container 10 held in an inverted state on the upper part of the seed container 10 mounted on the seed container mounting member 13. A mechanism 75 is provided. This seed container rotating mechanism 76 has a motor 76 attached to a fixed frame 58, and a disk 78 having a surface made of a material such as rubber attached to a tip of a rotating shaft 77 of the motor 76. By pressing a disc 78 rotated by a motor 76 against the bottom 10 B of the seed container 10, the seed container 10 is rotated by friction rotation of the disk 78. Things.

By doing so, the seed container 10 is rotated by the seed container rotating mechanism 75 with respect to the sterilization processing device 55 composed of a hot air generator fixedly disposed toward the mouth 1 OA of the seed container 10. By rotating, the entire periphery of the mouth 1 OA of the seed container 10 is exposed to the hot air from the hot air generator, and the entire periphery of the mouth 1 OA of the seed container 10 can be heated and sterilized.

In addition, the motor 76 of the inoculum container rotating mechanism 75 is attached to an elastic portion 79 provided as a part of the fixed frame 58, so that the inoculum pretreatment transport mechanism 56 removes the inoculum surface. Removal mechanism 5 2 Discs 8 elastically contact the bottom 10 B of the inoculum container 10 carried into the 2 position and transmit the rotational drive force of the motor 76 to the inoculum container 10 You can do it.

The disc 78 is inserted into the seed container 10 to remove the surface of the seed S by pressing and holding the bottom 10 B of the seed container 10 toward the seed container mounting member 13. This prevents the inoculum container 10 from rising from the inoculum container mounting member 13 against the pushing force of the inoculated bacterial blade 65.

Further, in the present embodiment, the sterilization treatment device 55 is configured such that the tip of the stone pipe 71 of the hot air generator, which is a heating means, is brought close to the position of the bacterial cutting blade 65 of the inoculum surface removing and removing mechanism 54. With this arrangement, in addition to the heat sterilization of the mouth 1 OA of the seed container 10 described above, heat sterilization of the bacteria cutting blade 65 can be performed.

Next, in the above-described configuration, the operation of the inoculation process using the normal inoculum inoculation device 11 will be briefly described.Here, in particular, the pretreatment device B for performing the pretreatment of the inoculum S provided in the inoculum inoculation device 11, namely, Aging in seed container 10 placed in an inverted state

Three The inoculum surface removal and removal mechanism 54 for removing the surface portion of the inoculum S and the sterilization apparatus 55 will be mainly described.

First, a container 7 containing a predetermined number of cultivation containers 6 with lids 5 is placed on the transfer conveyor 2 of the inoculum inoculation device and the container 7 is transferred, and the cultivation container 6 in the front row is inoculated with the inoculum. Then, the transfer conveyor 2 stops, and then the cultivation container 6 is positioned and fixed at the inoculum inoculation position while the vertical direction and the center position of the cultivation container 6 are regulated by the container positioning device 8.

At the time of positioning by the container positioning device 8, in this embodiment, the motor 35 as an ejection rotation drive member is also driven for a preset time, and the rotation of the ejection shaft 34 causes the inoculum S in the inoculum container 10 to rotate. The inoculum S is removed by the ejection blade 33, and the inoculated inoculum S is stored in the lower end portion of the inoculum hopper 42.

Next, after the positioning by the container positioning device 8 is performed, the lid 5 of the cultivation container 6 is rotated to the lid open position by the lid opening / closing device 9 with the operation of the cylinder 45, and interlocked with this lid opening operation. The inoculum supply cylinder 4 4 moves right above the cultivation container 6 from which the lid 5 has been removed, and the inoculum supply cylinder 4 4 is arranged on the axis connecting the inoculum hopper 4 2 and the cultivation container 6.

When the lid of the lid opening / closing device 9 is completely opened, by opening the shutter 43, the inoculum S in the inoculum hopper 4 2 is supplied into the cultivation container 6 in a horizontal row unit via the inoculum supply cylinder 44. After the supply of the inoculum S is completed, the shirt 43 returns to close the lower open end of the inoculum hopper 14 2, and the cylinder 45 supplies the inoculum supply cylinder 4 by the return operation. At the same time as 4 retracts and returns from the position of the cultivation container 6, the lid opening / closing device 9 rotates and returns, and covers the lids 5 of the cultivation containers 6 in a horizontal row inoculated with the inoculum S. In this embodiment, when the inoculum S in the inoculum container 10 becomes empty, the support member 17 is raised by the operation of the air cylinder 16. At the same time that the pressing support member 21 that presses the bottom portion 10B of 0 is released, the positioning shaft 27 that forms a part of the position restricting member 29 is also raised, and the fixing and pressing support of the seed container 10 is performed. Is released, and the positioning shaft 27 comes out of the fitting hole 28 provided in the inoculum container mounting member 13. As a result, the four inoculated inoculum containers 1 become empty. The seed loaded with 0

Four The bacteria container mounting member 13 is released from the position regulating member 29, thereby slidingly moving along the slope of the inclined rail-shaped guide member 14 and provided at the lower end of the slope of the guide member 14. The stopper portion 14 A is regulated by the stopper portion 14 A, and at the same time, the seed container mounting member 13 loaded with the four waiting seed cells 10 is next to the seed member S along the slope of the guide member 14.移行 Sliding transition to the discharge position occurs, and switching between the four inoculum containers 10 filled with inoculum S in the standby state and the four inoculated inoculum containers 10 is automatically performed. Done in

After the replacement operation of the seed container 10, when the piston of the cylinder 16 moves down, the support member 17 fixed to the piston descends, and is attached to the support member 17 with this descending operation. The positioning shaft 27 and the pressing plate 20 are lowered, and the tip of the positioning shaft 27 is inserted into the fitting hole 28 provided in the inoculum container mounting member 13. The bottoms 10 B of the four inoculum containers 10 newly supplied by 20 are pressed and supported, thereby preparing the extraction of the inoculum S in the various inoculum containers 10. As described above, the seed blade S is sequentially driven by rotating the blade 33 and the seed container 10 to rotate, and the inoculation operation is sequentially performed by filling the seed container S into the cultivation container 6. .

Therefore, by sequentially operating the above-described series of steps, the inoculum S can be continuously inoculated in the cultivation container 6 in the container 7 in a horizontal row unit.

By the way, in the above-described embodiment, the operation of the pretreatment device B including the inoculum surface scraping and removing mechanism 54 and the sterilization treatment device 55 provided in the inoculum inoculation device, which is the main object of the present invention, will be described.

In the inoculum inoculation device 11 described above, at the time of supplying the inoculum container 10 or at the same time as the switching operation of the inoculum container 10, from the inoculum container supply mechanism 46 installed on the loading side of the guide member 14, The inoculum container mounting member 13 placed on the endless chain 48 is intermittently fed by the rotation drive of the motor 49, which is the loading / supplying drive means, and the feeding end of the chain 48 is sent by this feeding. The seed container mounting member 13 placed on the part is newly fed into the supply side of the guide member 14 disposed at a predetermined angle.

At this time, in the middle of the guide member 14, as shown in FIG. 2 or FIG. An inoculum inoculation pretreatment transport mechanism 56 is provided, and on a mounting slide member 57 formed in a frame shape with a stopper for a pretreatment step constituting the inoculum inoculation pretreatment transport mechanism 56, The inoculum mounting member 13 is fed in and stocked at a predetermined position. In other words, the inoculum inoculation pretreatment transport mechanism 56 transports the inoculum container 10 that is in a standby state on the guide member 14 with respect to the inoculum extraction mechanism 32 of the inoculum inoculation device 11 and the inoculation position of the inoculum extraction mechanism 32 The seed container mounting member 13 is to be transported to the pretreatment device B together.

Next, with the rotation of the motor 61, as shown in FIG. 11 from the position shown in FIG. 10, as shown in FIG. 11, the guide member 14 is orthogonal to the feeding direction of the inoculum container mounting member 13 by the guide member 14. The seed container mounting member 13 placed on the slide member 57 described above along the direction along with the slider portion 60 via the guide rail 59 attached to the fixed frame 58 side at the final end. To the final position, and when it reaches the final end position, one of the pretreatment devices B disposed at the final end position in correspondence with the stop position of the inoculum container 10 on the near side. The above-mentioned inoculum surface removing and removing mechanism 54 is guided upward by the rotation of the motor 66 as well as the bacterial incision blade 65 of the inoculum container 10 from the mouth 1 OA of the inoculum container 10. Feed it inside and drive it up a specified amount of depth dimension, The surface of hard and aged inoculum S in 0 is removed.

At this time, the bottom 10 B of the seed container 10 arranged in an inverted state comes into contact with the disk 78 of the seed container rotating mechanism 75, and the seed container 10 is rotated by the rotation of the motor 76. Is rotated, and the sterilization apparatus 55, which is another pretreatment apparatus B, which is a hot air generation apparatus, is operated to move at least one seed container 10 with respect to the hot air blowing position of the fixedly disposed hot air processing apparatus. By rotating more than the rotation, the entire periphery of the mouth 1 OA of the seed container 10 is exposed to the hot air of the hot air generator that is the heating means, so the entire periphery of the mouth 10 A of the seed container 10 is heated and sterilized. You.

By doing so, even if the inoculum S whose surface has been scraped off and removed by the inoculum surface scraping and removing mechanism 54 touches the mouth 1 OA of the inoculum container 10 and various bacteria adhere thereto, The entire periphery of the mouth 1 OA of the inoculum container 10 is heated and sterilized by the sterilization treatment device 55, which is a heating means comprising the hot air generator, so that the inoculum to be used in the subsequent process Can prevent inoculation of inoculum s with various bacteria at the time of inoculation.

Subsequently, when the bacteria cutting blade 65 reaches a predetermined depth dimension from the mouth 1 OA of the seed container 10, that is, only the surface portion of the hardened and aged seed S in the seed container 10 is removed. When reaching the rising end, which is the depth to be removed, the motor 16 rotates in reverse so as to return to the descending end. With this reverse rotation, the germicidal removal screw shaft 6 4 and the germicidal blade 6 are rotated. 5 and motor 6 6 move down and return.

Further, at this time, before and after the removal of the surface portion of the hardened and aged inoculum S in the inoculum container 10, the inoculum blade of the inoculum surface removal and removal mechanism 54 is used. 65 is exposed to the hot air of a sterilization treatment device 55 which is a heating means comprising a hot air generator, and is sterilized by heating together with the mouth 1 OA of the inoculum container 10.

Subsequently, as shown in FIG. 12, the seed container 10 arranged in an inverted state, four of which are set in a horizontal line on the seed container mounting member 13, has a distance of one from the final end position. Is retracted along with the backward movement of the placing slide member 57, and in this state, the next inoculum container 10 is provided on the inoculum surface removing and removing mechanism 54 from the mouth 1OA of the inoculum surface 104. Similarly, only the surface of the hardened and aged inoculum S in the inoculum container 10 is removed by the blade 65 and the heat sterilization of the mouth 1 OA of the cultivation container 10 and the bacterial blade 65 is performed. In the following, the same operation is repeated as shown in FIGS. 13 and 14.

Therefore, in this embodiment, in the pretreatment stage of the inoculation process using the inoculum inoculation device 11 described above, the return of the inoculum inoculation pretreatment transport mechanism 56 causes the hardened inoculum S in the inoculum container 10 to be removed. A step of removing only the surface portion, and a step of removing the inoculum blade 65 and the inoculum container 10 which are a part of the inoculum surface removal and removal mechanism 54 used in the removal. The step of heating and sterilizing the vicinity of the mouth 10A can be performed in advance before inoculating the inoculum, so that the inoculum can be easily removed and removed simply by setting the inoculum container 10 in an inverted state. There is an effect that it is possible to provide a seed inoculum inoculating apparatus capable of performing a pretreatment such as a sterilization treatment.

In this case, after the completion of the above-described operation of the pretreatment step of inoculating the seed with the pretreatment device B, the seed container 10 placed on the mounting slide member 57 and returned to the guide member 14 position 10 Seed container mounting member 1 3 with the inverted mounted The release of the stopper for the pretreatment process provided on the member 57 causes the inoculation container mounting member 13 to move to the position where the inoculum S of the inoculum inoculation device 11 is extracted, and the piston of the cylinder 16 With the lowering operation, the supporting member 17 fixed to the piston descends, and with this lowering operation, the positioning shaft 27 attached to the supporting member 17 and the pressing plate 20 descend to position. The tip of the shaft 27 is inserted into the fitting hole 28 provided in the inoculum container mounting member 13, and the bottom 10 B of the four inoculum containers 10 newly supplied by the pressing plate 20 is formed. Thus, the seeds S are prepared for ejection in the various bacteria containers 10, and then the seed blades S are rotated by the rotation of the extraction blade 33 and the seed containers 10 as described above. The pumping operation is performed sequentially, and the cultivation container 6 The inoculation work is performed sequentially by filling the inside with the inoculum S, whereby the inoculum container 10 is transported to the pretreatment device B with the inoculum container 10 set in an inverted state, and the inoculum container 1 Aging operation of the aged inoculum S in 0, sterilization of the inoculum of the inoculum container 1 0 near the mouth 1 OA, and sterilization of the inoculum blade 6 5 that performs the inoculum operation be able to.

At this time, in the inoculum container mounting member 13 provided in connection with the guide member 14, the inoculum inoculation pretreatment transport mechanism 56 is in a standby state at a pre-process position of the inoculum inoculation by the inoculum ejection mechanism 32. The pre-treatment device B is transported to the pretreatment device B, and after being subjected to a predetermined pre-treatment, is returned to the guide member 14 to be transported. (1) Inoculation mechanism for inoculum (3) Inoculation container for inoculum at position (2) (1) Inoculation process for inoculum (10) as it proceeds simultaneously while exposing to inoculation container (10) It does not affect.

In addition, since these pretreatment steps are performed with the neck of the inoculum container 10 turned upside down, there is also an effect that the invasion of dust and other germs can be favorably prevented.

In this example, a single pretreatment device B was provided for the inoculum container 10 in a horizontal row mounted on the inoculum container attachment member 13, and the inoculum container 1 was sequentially attached to the pretreatment device B. Pre-treatment of various bacteria containers 10 can be performed by sending 0, and it can be configured inexpensively at low cost.For example, the inoculation of inoculum S by the inoculum ejection mechanism 32 If the inoculum S is consumed quickly and the inoculum S in the inoculum container 10 disappears quickly, the switching time of the inoculum container 10 In order to shorten the switching time of the attaching member 13, a plurality of pretreatment devices B may be provided in the lateral direction (transfer direction of the inoculum pretreatment transport mechanism) to increase the efficiency of the pretreatment work.

Further, in the embodiment, the heating means including the hot air generator is applied as the sterilization processing apparatus, but a sterilization processing mechanism including the heating means such as an alcohol lamp and a gas burner may be used. Further, as a means that does not use heat, not limited to the heating means, for example, a sterilization treatment apparatus for sterilizing by spraying alcohol or a sterilizing chemical may be provided. Also, if it is desired to directly feed the inoculum container mounting member 13 in the standby state to the inoculum ejection mechanism 32 without performing the pretreatment by the pretreatment device, the switching of the pretreatment device B by the not-shown switching switch. By stopping the function and not driving the inoculum inoculation pretreatment transport mechanism 56, the stopper for the pretreatment step is always kept open, so that the guide member 14 can be used without pretreatment. The pre-treatment can be activated or deactivated easily. Industrial applicability

The present invention relates to an inoculum inoculation apparatus for inoculating a culture medium filled in a cultivation container or a cultivation packaging bag with a mushroom inoculum, and adding a pretreatment apparatus for performing a predetermined pretreatment to the inoculum container before inoculation. By configuring the device, the added value of the inoculum inoculation device can be improved.Pretreatment other than the aforementioned sterilization treatment of OA 1 Arbitrary processing such as opening the lid of 10 and cleaning of the mouth 1OA can also be performed.

9

Claims

The scope of the claims

1. On the body frame, the mouth side of the seed container is placed upside down, and a seed discharging mechanism is provided to discharge a predetermined amount of seeds such as mushrooms contained in the seed container. A seed inoculation device configured to supply the seeds extracted by the seeding mechanism into a cultivation container or a cultivation packaging bag filled with a culture medium. And a pretreatment device for pretreating the seed container is provided.

2. The pretreatment device according to claim 1, wherein the pretreatment device comprises a seed fungus surface removing mechanism having a fungal cutting blade for removing a surface portion of the inoculum in the inoculum container. Seed inoculation device.

3. The inoculum inoculation device according to claim 2, further comprising a sterilization treatment device that sterilizes the fungal cutting blade of the inoculum removal and removal mechanism.

4. The inoculum inoculation device according to claim 1, wherein the pretreatment device comprises a sterilization treatment device for sterilizing the mouth of the inoculum container.

5. The pretreatment device is a seed fungus surface removing and removing mechanism having a fungal cutting blade for removing a surface portion of the inoculum in the inoculum container, and a sterilization processing device for sterilizing the mouth of the inoculum container. The inoculum inoculation according to claim 1, wherein the sterilization treatment device simultaneously performs the sterilization treatment on the blades of the seed removal and removal mechanism.

6. The inoculum inoculation device according to claim 4, further comprising a seed container rotation mechanism for rotating the seed container.

7. The sterilization apparatus according to claim 3, wherein the sterilization apparatus comprises heating means. Item 7. The inoculum inoculation device according to any one of items 6 to 6.

8. Inoculum container mounting member for disposing the inoculum container in an inverted state, and a inoculum ejection mechanism for ejecting a predetermined amount of inoculum such as mushrooms contained in the inoculum container mounted on the inoculum container mounting member. A guide member capable of sequentially and intermittently supplying the inoculum container mounting member to the inoculum ejection mechanism position; and a side from the guide member in a direction orthogonal to a transfer direction of the inoculum container mounting member by the guide member. A pretreatment device that is provided so as to protrude toward the front and performs pretreatment of the seed container, and a seed inoculation pretreatment transport mechanism that reciprocates the seed container mounting member between the guide member and the pretreatment device position. 2. The inoculum inoculation device according to claim 1, further comprising:

9. The inoculum pretreatment device in the inoculum inoculation device according to claim 8, wherein the inoculum container mounting member arranges a plurality of the inoculum containers in a horizontal row.

10. The seed member container mounting member, which is in a standby state at the pre-step position of the inoculum inoculation by the inoculation device in the guide member, is transported to the pretreatment device position by the inoculum inoculation pretreatment transport mechanism. 10. The inoculum inoculating device according to claim 8, wherein after performing a predetermined pretreatment, the device is conveyed back to the guide member.

Two