WO1998056811B1 - Plant grab proteins - Google Patents
Plant grab proteinsInfo
- Publication number
- WO1998056811B1 WO1998056811B1 PCT/EP1998/003662 EP9803662W WO9856811B1 WO 1998056811 B1 WO1998056811 B1 WO 1998056811B1 EP 9803662 W EP9803662 W EP 9803662W WO 9856811 B1 WO9856811 B1 WO 9856811B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- grab
- peptide
- protein
- seq
- sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Abstract
A method of controlling plant cell and plant virus growth and/or replication, plant cell cycle, differentiation, development and/or scenescence is provided characterised in that it comprises increasing or decreasing the levels or binding capabilities of GRAB (Geminivirus RepA Binding) proteins other than Rb (Retinoblastoma) proteins within plant cells.
Claims
1. A method of controlling plant cell cycle characterised in that it comprises increasing or decreasing the levels of, or Geminivirus RepA binding capabilities of, GRAB (Geminivirus RepA Binding) protein or peptide within a plant cell characterised in that the GRAB protein or peptide comprises domains Nl, N2, N3, N4 and N5 shown in Figure 5 herein which have the designated amino acids shown in SEQ ID No 3 and SEQ ID 4.
2. A method as claimed in claim 1 characterised in that the control of the plant cell cycle comprises one or more of control of plant cell or plant virus growth and/or replication, plant cell differentiation, development and/or scenescence.
3. A method as claimed in any one of the preceding claims wherein the GRAB proteins or peptides have a first 150 N-terminal amino acids capable of binding to viral
RepA protein.
4. A method as claimed in any one of the preceding claims characterised in that the GRAB proteins or peptide comprises an amino acid sequence SEQ ID No 3 or 4 as shown herein or a functional variant thereof that is capable of binding Geminivirus RepA.
5. A method as claimed in any one of the preceding claims characterised in that it comprises overproducing or underproducing the protein or peptide in a plant cell.
51
6. A method as claimed in any one of claims 1 to 5 characterised in that it comprises decrease of native GRAB binding activity by application of an agent that binds to GRAB protein or peptide.
7. A method as claimed in any one of the preceding claims characterised in that the GRAB proteins or peptides have amino acid sequence homology of at least 70% with that of SEQ ID No 3 or 4.
8. A method as claimed in any one of the preceding claims comprising placing of the corresponding GRAB protein or peptide encoding or antisense nucleotides within the plant cell.
9. A method as claimed in claim 8 characterised in that the nucleotides are in the form of recombinant nucleic acid comprising a GRAB protein or peptide encoding sequence.
10. A method as claimed in claim 9 characterised in that the sequence is positioned behind a promotor capable of supporting GRAB protein or peptide expression or production of antisense RN A.
1 1 . A method as claimed in any one of claims 1 to 10 characterised in that the protein or peptide is applied or produced ectopically.
52
12. A method as claimed in claim 1 1 characterised in that the tissue is vegetative tissue or stem tissue.
13. A method as claimed in any one of the preceding claims comprising expressing a protein or peptide that is capable of binding to GRAB protein or peptide or functional variant thereof within the cell.
14. A method as claimed in any preceding claim characterised in that it comprises downregulating native GRAB expression by gene silencing coexpression or through antisense strategy.
15. A method as claimed in any one of the preceding claims characterised in that it comprises producing or inhibiting senescence in a plant cell comprising increasing or decreasing the levels or binding activity of a GRAB protein or peptide comprising a sequence of SEQ ID No 10 or a functional variant therof capable of inducing senescence in N.bentamiana plants, in a plant cell.
16. A method as claimed in claim 15 comprising incorporation of nucleic acid encoding RepA, N-terminal truncated RepA or a functional variant of one of these.
17. A GRAB protein or peptide per se, or in enriched, isolated, cell free and/or recombinantly produced form characterised in that characterised in that the GRAB protein or peptide comprises domains Nl , N2, N3, N4 and N5 shown in Figure 5 herein which have the designated amino acids shown in SEQ ID No 3 and SEQ ID 4 and with the proviso that it is not one of SENU, NAM, ATAF 1 or ATAF2.
18. A protein or peptide as claimed in claim 17 characterised in it has an N-terminal sequence having 90% or more homology to the first 150 N-terminal amino acids of GRAB 1 or GRAB2 described herein or conservatively substituted variants thereof.
19. A GRAB protein or peptide as claimed in claim 17 characterised in that it comprises an amino acid sequence SEQ ID No 3 or 4 as shown or a functional variant thereof having an amino acid sequence of homology of at least 70% with that sequence that is capable of binding Geminivirus RepA.
20. A protein or peptide as claimed in claim 19 characterised in that it comprises a sequence of SEQ ID No 6 or 8 or a functional variant thereof having an amino acid sequence of homology of at least 70% with that sequence.
21. A protein or peptide as claimed in claim 20 characterised in that it comprises a sequence of SEQ ID No 10 or 12 or a functional variant thereof having an amino acid sequence of homology of at least 70% with that sequence.
22. A GRAB protein or peptide encoding or antisense nucleic acid per se, or in enriched, isolated, cell free and/or recombinant form characterised in that the GRAB protein or peptide comprises domains Nl . N2, N3. N4 and N5 shown in Figure 5 herein which have the designated amino acids shown in SEQ ID No 3 and SEQ ID 4 and with the proviso that it does not encode the full amino acid sequence of SENU, NAM, ATAF1 or ATAF2.
23. Nucleic acid as claimed in claim 22 characterised in that it is in the form of recombinant DNA or cRNA (mRNA) that codes for the expression of a GRAB protein or peptide having an N-terminal sequence with at least 60% homology with the first 200 N-terminal amino acids of GRAB 1 or GRAB2 as described herein
54
24. A nucleic acid as claimed in claim 23 characterised in that it is a DNA or RNA polynucleotide comprising one or more of SEQ ID No 1, 2, 5, 7, 9 or 1 1 or a functional variant thereof
25. A method of producing a protein or peptide as claimed in any one of claims 18 to 23 characterised in that it comprises expressing DNA or RNA as described in claim 23 or 24.
26. A nucleic acid probe or primer characterised in that it comprises an oligonucleotide or polynucleotide of sequence complementary to any 15 or more contiguous bases of the DNA sequences identified herein below as SEQ ID No 5, 7, 9 or 1 1 or complemetary sequences or RNA sequences corresponding thereto.
27. A nucleic acid transformation vector characterised in that it comprises DNA or RNA as described in any one of claims 8 to 16 or 22 to 24.
28. A method for producing transformed cells comprising nucleic acid as claimed in or described in any one of claims 8 to 16 or 22 to 24 comprising introducing said nucleic acid into the cell in vector or free form.
29. A method as claimed in claim 28 characterised in that the nucleic acid is introduced directly by electroporation or particle bombardment.
30. A cell comprising recombinant nucleic acid as described or claimed in any one of claims 8 to 16 or 22 to 24.
J i . A transgenic plant or part thereof comprising a cell as claimed in claim 30.
32. A plasmid containing a DNA coding for expression of GRAB protein GRAB 1 or GRAB 2 described herein as deposited under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms of 1977; these being
55
56
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002289863A CA2289863A1 (en) | 1997-06-12 | 1998-06-09 | Plant grab proteins |
| AU82160/98A AU753798B2 (en) | 1997-06-12 | 1998-06-09 | Plant grab proteins |
| BR9809447-5A BR9809447A (en) | 1997-06-12 | 1998-06-09 | Plant grab proteins |
| NZ500529A NZ500529A (en) | 1997-06-12 | 1998-06-09 | Modifying GRAB1 and or GRAB2 (Geminivirus RepA Binding) to affect cell plant cycle using antisense sequences |
| EP98932162A EP0989997A2 (en) | 1997-06-12 | 1998-06-09 | Plant grab proteins |
| JP50164599A JP2002506345A (en) | 1997-06-12 | 1998-06-09 | Plant GRAB protein |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009701292A ES2132025B1 (en) | 1997-06-12 | 1997-06-12 | URAG PROTEINS OF PLANTS. |
| ESP9701292 | 1997-06-12 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| WO1998056811A2 WO1998056811A2 (en) | 1998-12-17 |
| WO1998056811A3 WO1998056811A3 (en) | 1999-03-04 |
| WO1998056811B1 true WO1998056811B1 (en) | 1999-04-08 |
| WO1998056811A8 WO1998056811A8 (en) | 2000-04-27 |
Family
ID=8299674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP1998/003662 WO1998056811A2 (en) | 1997-06-12 | 1998-06-09 | Plant grab proteins |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0989997A2 (en) |
| JP (1) | JP2002506345A (en) |
| CN (1) | CN1260837A (en) |
| AU (1) | AU753798B2 (en) |
| BR (1) | BR9809447A (en) |
| CA (1) | CA2289863A1 (en) |
| ES (1) | ES2132025B1 (en) |
| NZ (1) | NZ500529A (en) |
| WO (1) | WO1998056811A2 (en) |
| ZA (1) | ZA985135B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6284947B1 (en) | 1999-02-25 | 2001-09-04 | Pioneer Hi-Bred International, Inc. | Methods of using viral replicase polynucleotides and polypeptides |
| DE69919406T2 (en) * | 1999-02-11 | 2005-09-08 | Temasek Life Sciences Laboratory Ltd. | NAC1- A PLANT ENGINE FOR A TRANSCRIPTION FACTOR INVOLVED IN THE DEVELOPMENT OF COTYLEDONES AND SIDE ROOTS |
| US6770800B2 (en) | 1999-03-12 | 2004-08-03 | Pioneer Hi-Bred International, Inc. | Methods of using viral replicase polynucleotides and polypeptides |
| GB9923306D0 (en) | 1999-10-01 | 1999-12-08 | Isis Innovation | Diagnostic and therapeutic epitope, and transgenic plant |
| FR2806095A1 (en) * | 2000-03-10 | 2001-09-14 | Gentech | New polynucleotides for producing transgenic plants resistant to geminivirus infection comprising polynucleotides encoding proteins which interact with at least one of the products of the geminivirus genome |
| US20020188965A1 (en) * | 2001-04-20 | 2002-12-12 | Zou-Yu Zhao | Methods of transforming plants |
| GB0212885D0 (en) | 2002-06-05 | 2002-07-17 | Isis Innovation | Therapeutic epitopes and uses thereof |
| US10105437B2 (en) | 2004-04-28 | 2018-10-23 | Btg International Limited | Epitopes related to coeliac disease |
| NZ550600A (en) | 2004-04-28 | 2010-03-26 | Btg Int Ltd | Epitopes related to coeliac disease |
| JP4734959B2 (en) * | 2005-02-25 | 2011-07-27 | 味の素株式会社 | A novel plasmid capable of autonomous replication in microorganisms belonging to the family Enterobacteriaceae |
| CN106086005A (en) * | 2016-08-22 | 2016-11-09 | 宁夏农林科学院 | A kind of Wheat DNA rapid extracting method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2257972A1 (en) * | 1996-06-13 | 1997-12-18 | Consejo Superior De Investigaciones Cientificas | Plant proteins |
-
1997
- 1997-06-12 ES ES009701292A patent/ES2132025B1/en not_active Expired - Lifetime
-
1998
- 1998-06-09 CA CA002289863A patent/CA2289863A1/en not_active Abandoned
- 1998-06-09 WO PCT/EP1998/003662 patent/WO1998056811A2/en not_active Application Discontinuation
- 1998-06-09 EP EP98932162A patent/EP0989997A2/en not_active Withdrawn
- 1998-06-09 BR BR9809447-5A patent/BR9809447A/en not_active IP Right Cessation
- 1998-06-09 AU AU82160/98A patent/AU753798B2/en not_active Ceased
- 1998-06-09 JP JP50164599A patent/JP2002506345A/en active Pending
- 1998-06-09 NZ NZ500529A patent/NZ500529A/en unknown
- 1998-06-09 CN CN98805828A patent/CN1260837A/en active Pending
- 1998-06-12 ZA ZA9805135A patent/ZA985135B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2132025A1 (en) | 1999-08-01 |
| AU8216098A (en) | 1998-12-30 |
| ES2132025B1 (en) | 2000-12-01 |
| JP2002506345A (en) | 2002-02-26 |
| WO1998056811A3 (en) | 1999-03-04 |
| CA2289863A1 (en) | 1998-12-17 |
| NZ500529A (en) | 2001-10-26 |
| ZA985135B (en) | 1999-12-13 |
| CN1260837A (en) | 2000-07-19 |
| WO1998056811A2 (en) | 1998-12-17 |
| AU753798B2 (en) | 2002-10-31 |
| WO1998056811A8 (en) | 2000-04-27 |
| EP0989997A2 (en) | 2000-04-05 |
| BR9809447A (en) | 2000-06-20 |
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