WO1998056786A1

WO1998056786A1 - 2-aminoalkylaminoquinolines as dopamine d4 ligands

Info

Publication number: WO1998056786A1
Application number: PCT/US1998/014235
Authority: WO
Inventors: Xiao-Shu He
Original assignee: Neurogen Corporation
Priority date: 1997-06-13
Filing date: 1998-06-11
Publication date: 1998-12-17
Also published as: CA2293480A1; EP0991642A1; AU8296998A; JP2002504125A

Abstract

A compound of formula (IA) or the pharmaceutically acceptable acid addition salts thereof wherein: R1, R2, R3, R4 and R5 are the same or different and represent hydrogen, halogen, C1-C6alkyl, C1-C4alkoxy, C1-C4alkylthio, hydroxy, amino, mono(C1-C6) alkylamino, di(C1-C6)alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy; R6 is hydrogen or C1-C6alkyl; W is nitrogen, COH, or CH; Y and Z independently represent nitrogen or CH; and A represents an alkylene group of from 2-5 carbon atoms optionally substituted with one or more alkyl groups having from one to four carbon atoms. The compounds of the invention demonstrate high affinity and selectivity in binding to the D4 receptor subtype.

Description

2-AMINOALKYLAMINOQUINOLINES AS DOPAMINE D₄ LIGANDS

BACKGROUND OF THE INVENTION

Field of the invention

This invention relates to aminoquinoline derivatives which selectively bind to brain dopamine receptor subtypes. More specifically, it relates to 2- quinolyl(azacycloalkylalkyl)amines and pharmaceutical compositions and preparations containing such compounds. It also relates to the use of such compounds in the treatment or prevention of neuropsychochological disorders such as schizophrenia and other central nervous system diseases.

Description of the Related Art The therapeutic effect of conventional antipsychotics, known as neuroleptics, is generally believed to be exerted through blockade of dopamine receptors. However, neuroleptics are frequently responsible for undesirable extrapyramidal side effects (EPS) and tardive dyskinesias, which are attributed to blockade of D2 receptors in the striatal region of

the brain. The dopamine D4 receptor subtype has recently been identified. See Nature 350:

610 (Van Tol et al, 1991) and Nature, 347: 146 (Sokoloff et al., 1990) Its unique localization in limbic brain areas and its differential recognition of various antipsychotics suggest that the D4 receptor plays a role in the etiology of schizophrenia. Consequently, selective D4

antagonists are considered effective antipsychotics free from the neurological side effects displayed by conventional neuroleptics. U.S. Patent No. 5,093,333 describes N-substituted-2-aminoquinolines said to be Mi receptor agonists. SUMMARY OF THE INVENTION

This invention provides novel compounds of Formula I which interact with dopamine receptor subtypes. A broad aspect of the invention is directed to compounds of Formula I:

I wherein:

Ri, R₂, and R₃ independently represent hydrogen, halogen, C]-C₆ alkyl, Cj-C alkoxy, Cj-C alkylthio, hydroxy, amino, mono(C,-C₆) alkylamino, di(Cι-C₆) alkylamino, cyano,

nitro, trifluoromethyl or trifluoromethoxy; R is hydrogen or Cι-C₆ alkyl; and

Q represents a substituted azacycloalkylalkyl group of the formula:

where

W is nitrogen, CH or COH;

A represents an alkylene group of from 2-5 carbon atoms optionally substituted with one or more alkyl groups having from one to four carbon atoms; and T is an aryl or heteroaryl moiety optionally substituted with up to two groups selected from hydrogen, halogen, Cι-C₆ alkyl, C₁-C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino,

mono(C,-C₆) alkylamino, di(Cι-C₆) alkylamino, cyano, nitro, trifluoromethyl and

trifluoromethoxy. In yet another aspect, the invention provides pharmaceutical compositions comprising compounds of Formula I.

Since dopamine D₄ receptors are concentrated in the limbic system (Taubes, Science

265: 1034, 1994) which controls cognition and emotion, compounds which interact with these receptors are useful in the treatment of cognitive disorders. Such disorders include cognitive deficits which are a significant component of the negative symptoms (social withdrawal and unresponsiveness) of schizophrenia. In addition, disorders involving memory impairment or attention deficit disorders can be treated with the compounds of this invention. These compounds interact specifically with the dopamine D receptor subtype.

The compounds of the invention demonstrate high affinity and selectivity in binding to the D4 receptor subtype. The use of the compounds of this invention in methods of treating

neuropsychological disorders is predicated on the ability of the compounds to bind selectively to a dopamine receptor subtype, the D₄ receptor. The compounds of the invention can therefore be used in the treatment of schizophrenia, psychotic depression and mania. Other dopamine-mediated diseases such as Parkinsonism and tardive dyskinesias can also be treated directly or indirectly by modulation of D4 receptors.

Thus, in another aspect, the invention provides methods for treating and/or preventing neuropsychological disorders including, for example, schizophrenia, mania, dementia, depression, anxiety, compulsive behavior, substance abuse, memory impairment, cognitive deficits, Parkinson-like motor disorders and motion disorders related to the use of neuroleptic agents. It also provides methods of treating affective disorders such as Alzheimer's disease and certain movement disorders such as Parkinsonism and dystonia.

The invention further provides methods for treating the extrapyramidal side effects associated with the use of conventional neuroleptic agents. The compounds of the present invention are also useful for the treatment of other disorders which respond to dopaminergic blockade such as substance abuse and obsessive compulsive disorder.

DETAILED DESCRIPTION OF THE INVENTION In addition to the compounds of Formula I above, the invention encompasses compounds of Formula I A:

IA wherein:

Ri, R₂, R₃, R4 and R₅ are the same or different and represent hydrogen, halogen, C]-C₆ alkyl,

C]-C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino, mono(C,-C₆) alkylamino, di(C]-C₆) alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy; Re is hydrogen or Cι-C₆ alkyl; W is nitrogen, COH, or CH;

Y and Z independently represent nitrogen or CH; and

A represents an alkylene group of from 2-5 carbon atoms optionally substituted with one or more alkyl groups having from one to four carbon atoms.

In Formula IA, the dashed segment represents either a single bond resulting in a 3,4 dihydroquinoline; or a double bond resulting in a quinoline.

In the compounds of the invention, "W" preferably represents nitrogen or COH.

Preferred "T" groups in Formula I are 6-membered carbocyclic aromatic ring systems having zero, one or two nitrogen atoms. Particularly preferred "T" groups are phenyl, 2- pyridinyl, and 2-pyrimidinyl. The particularly preferred "T" groups are optionally mono- or disubstituted with halogen, Ci-Cβ alkyl, -C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino,

mono(C_rC₆) alkylamino, di(Cι-Ce) alkylamino, cyano, nitro, trifluoromethyl or

trifluoromethoxy .

Preferred compounds of Formula IA are those where Rg is hydrogen, methyl or

ethyl.

Preferred "T" groups in Formula I are

The invention also provides compounds of Formula II:

II wherein:

Ri, R₂, R₃, R4 and R₅ are the same or different and represent hydrogen, halogen, Cι-C₆ alkyl,

C₁-C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino, mono(C_rC₆) alkylamino, di(Cι-C₆)

alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy; R₆ is hydrogen or Cι-C₆ alkyl;

W is nitrogen, COH, or CH; Y and Z independently represent nitrogen or CH; and A represents an alkylene group of from 2-5 carbon atoms optionally substituted with one or^~ more alkyl groups having from one to four carbon atoms.

Preferred compounds of Formula II are those where R is hydrogen, methyl or ethyl. Particularly preferred compounds of Formula II are those where

In addition, the invention encompasses compounds of Formula III:

III wherein:

C₁-C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino, mono(C C₆) alkylamino, di(Cι-C )

alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy; R₆ is hydrogen or -Cβ alkyl; W is nitrogen, COH, or CH;

Y and Z independently represent nitrogen or CH; and

In preferred compounds of Formula III, Rg is hydrogen, methyl or ethyl. In certain situations, compounds of Formula I may contain one or more asymmetric ^"" carbon atoms, so that the compounds can exist in different stereoisomeric forms. These compounds can be, for example, racemates or optically active forms. For example, where R₆ in Formula I is a methyl group, the resulting compound can be present as (R) and (S) stereoisomers. In these situations, the single enantiomers, i.e., optically active forms, can be obtained by asymmetric synthesis or by resolution of the racemates. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral HPLC column. Representative compounds of the present invention, which are encompassed by

Formula I, include, but are not limited to the compounds in Table I and their pharmaceutically acceptable salts. If the compound of the invention is obtained as an acid addition salt, the free base can be obtained by basifying a solution of the acid salt. Conversely, if the product is a free base, an addition salt, particularly a pharmaceutically acceptable addition salt, may be produced by dissolving the free base in a suitable organic solvent and treating the solution with an acid, in accordance with conventional procedures for preparing acid addition salts from base compounds.

Non-toxic pharmaceutically acceptable salts include salts of acids such as hydrochloric, phosphoric, hydrobromic, sulfuric, sulfinic, formic, toluenesulfonic, methanesulfonic, nitric, benzoic, citric, tartaric, maleic, hydroiodic, alkanoic such as, for example, acetic, HOOC-(CH2)n-COOH where n is 0-4, such as, for example, oxalic (n=0),

and the like. Those skilled in the art will recognize a wide variety of non-toxic pharmaceutically acceptable addition salts.

The present invention also encompasses the acylated prodrugs of the compounds of Formula I. Those skilled in the art will recognize various synthetic methodologies which may be employed to prepare non-toxic pharmaceutically acceptable addition salts and acylated^" prodrugs of the compounds encompassed by Formula I.

By the terms (C,-C₆)alkyl and lower alkyl is meant straight and branched chain alkyl groups having from 1-6 carbon atoms as well as cyclic alkyl groups such as, for example, cyclopropyl, cyclobutyl, or cyclohexyl. Specific examples of such alkyl groups are methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl, neopentyl and n-pentyl.

Preferred C_rC₆ alkyl groups are methyl, ethyl, propyl, butyl or cyclopropylmethyl.

By the terms (C,-C₆)alkoxy and lower alkoxy is meant straight and branched chain alkoxy groups having from 1-6 carbon atoms. By hydroxy C_rC₆ alkyl is meant a C,-C₆ alkyl group carrying a terminal hydroxy moiety.

By halogen, halo, or halide is meant fluorine, chlorine, bromine and iodine substituents.

The binding characteristics of compounds of Formula I for the D₄ receptor, expressed in nM, generally range from about 0.5 nanomolar (nM) to about 50 nanomolar (nM). These compounds typically have binding constants for the D₂ receptor of from at least about 100 nM to more than 3000 nM. Thus, the compounds of the invention are generally at least about 3, preferably at least about 5, and most preferably at least about 10 time more selective for the

D₄ receptor than the D₂ receptor. Even more preferably, these compounds are at least 20, and more preferably at least 25-50, times more selective for the D₄ receptor than the D₂ receptor.

As noted above, the invention also pertains to the use of compounds of general

Formula I in the treatment of neuropsychological disorders.

The compounds of general Formula I may be administered orally, topically, parenterally, by inhalation or spray or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal injection or infusion techniques. In addition, there is provided a pharmaceutical^" formulation comprising a compound of general Formula I and a pharmaceutically acceptable carrier. One or more compounds of general Formula I may be present in association with one or more non-toxic pharmaceutically acceptable carriers and/or diluents and/or adjuvants and if desired other active ingredients. The pharmaceutical compositions containing compounds of general Formula I may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs.

Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets. These excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monosterate or glyceryl distearate may be employed.

Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil. Aqueous suspensions contain the active materials in admixture with excipients ^" suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydropropyl methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p- hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin. Oily suspensions may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.

Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example sweetening, flavoring and coloring agents, may also be present. Pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monoleate, and condensation products of the and partial esters with ethylene oxide, for example polyoxyethylene sorbitan monoleate. The emulsions may also contain sweetening and flavoring agents.

Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitor or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents. The pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleaginous suspension. This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above. The sterile injectable preparation may also be sterile injectable solution or suspension in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

The compounds of general Formula I may also be administered in the form of suppositories for rectal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.

Such materials are cocoa butter and polyethylene glycols. Compounds of general Formula I may be administered parenterally in a sterile medium. The drug, depending on the vehicle and concentration used, can either be suspended or dissolved in the vehicle. Advantageously, adjuvants such as local anesthetics, preservatives and buffering agents can be dissolved in the vehicle. Dosage levels of the order of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions (about 0.5 mg to about 7 g per patient per day). The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. Dosage unit forms will generally contain between from about 1 mg to about 500 mg of an active ingredient.

It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy.

Preparation of 2-A-minoalkylaminoquinolines

The compounds of Formula I, and the pharmaceutically acceptable acid addition salts thereof, may be prepared according to the reactions shown below in Schemes I and II.

Scheme 1

wherein Ri-Rβ, W, Y and Z are as defined above for Formula I.

As shown in Scheme I, a quinoline of general structure IV, possessing an appropriate leaving group (X) at the 2 position, e.g., a halogen or S-methyl group, may be reacted with a primary or secondary amine of general structure V in the presence of a base to afford a compound of Formula I as the desired product. The reaction may be carried out at elevated temperature with or without a solvent. Further, the reaction mixture may also contain an acid scavenger such as diisopropylamine or an inorganic salt such as ammonium chloride. Where they are not commercially available, the compounds of general structure IV may be prepared by literature procedures or procedures analogous to those described in the literature. Compounds of general structure V are either known or capable of being prepared by the methods known in the art. Those having skill in the art will recognize that the starting materials may be varied and additional steps employed to produce compounds encompassed by the present invention.

Alternatively, compounds of the invention may be prepared according to the reactions shown in Scheme II. Thus, compounds may be prepared from readily available substituted or unsubstituted 4-haloquinoline compounds by allowing them to react with a Wittig reagent, such as an alkyl triphenylphosphonate generated from an alkyltriphenylphosphonium halideM plus a base, such as n-butyllithium in an organic solvent, such as tetrahydrofuran. The resulting 4-alkylquinoline can be converted to a 4-alkyl-2-haloquinoline by treatment with an oxidizing agent, such as m-chloroperbenzoic acid (MCPBA) in an appropriate solvent, such as chloroform, to give the corresponding 4-alkylquinoline N-oxide, followed by reaction with a halogenating agent, such as phosphorus oxychloride to give a 4-alkyl-2-haloquinoline of Formula IVa. Reaction of 2-haloquinoline IVa with amines of Formula V, as described above, yields compounds of this invention.

Scheme II

wherein R_t-R₆ are as defined above, Rj is alkyl of 1 to 4 carbon atoms, and X is halogen. The disclosures in this application of all articles and references, including patents, are incorporated herein by reference.

Those having skill in the art will recognize that the starting materials may be varied and additional steps employed to produce compounds encompassed by the present invention. The invention is illustrated further by the following examples which are not to be construed as limiting the invention in scope or spirit to the specific procedures and compounds described in them.

Example 1 Preparation of l-fPyrimidin-2-yl.-4-(2-aminoethyl.piperazine

A mixture of N-(2-bromoethyl)phthalimide (50.8 g, 0.2 mole), l-(pyrimidin-2- yl)piperazine (32.8 g, 0.2 mole) and potassium carbonate (55.2 g, 0.4 mole) in dimethyl formamide (400 mL) is heated at 80°C for 16 hours under a nitrogen atmosphere. After cooling, the reaction mixture is poured into water (1 L) and ether. (1 L). The heterogeneous mixture is then filtered to remove solids and the layers separated. The aqueous layer is further extracted with ether (2 X 300 mL). The combined organic layers are dried (Na₂SO₄) and concentrated to provide colorless crystals (65 g, 96 %, m.p. 132-133°C).

A portion of the crystals (5 g, 15 mmol) is refluxed under nitrogen in hydrazine hydrate (50 mL) for 7 h. After cooling, the solution is poured into 30% potassium carbonate solution (50 mL) and extracted with methylene chloride. The organic extracts are dried (Na₂SO₄) and concentrated to give a pale yellow oil (3.27 g). This oil is dissolved in

methanol (5 mL) and combined with a methanolic solution (10 mL) of fumaric acid (3.66 g). Isopropanol is then added (50 mL) and the resulting mixture concentrated on a hot plate to a volume of 20 mL. Upon cooling, the crystals of fumarate salt are collected (4.77 g, 99%, m.p. 260 °C dec) Example 2 Preparation of ,2-(4-pyrimidin-2-ylpiperazinyl, ethyl, -2-quinolylamine (Compound 1 .

A solution of l-(pyrimidin-2-yl)-4-(2-aminoethyl)piperazine (1 g) in xylene (200 mL) is treated with 2-chloroquinoline (1.5 g) and potassium carbonate (2 g). The mixture is then refluxed under N₂ overnight. After cooling, the solution is diluted with diethyl ether (200

mL) and washed with water (3 X 50 mL). The organic layer is then extracted with an aqueous solution of 10% acetic acid. The aqueous extract is subsequently washed with ether (50 mL), basified with 50% NaOH solution and extracted with ether. The ether layer is dried (K₂CO₃)

and evaporated to give the product as an oil (0.47 g). The oil is dissolved in ethanol (20 mL), treated with 48% HBr until acidic and concentrated to a volume of approx. 5 mL. Upon cooling, the off- white crystalline hydrobromide salt is collected by filtration (0.45 g, mp 174-

176 °C).

Example 3

Preparation of 2-3-4-dihvdroquinolyl(2-(4-(2-pyridyl,piperazinvDethyl)amine (Compound 2.

A solution of 3,4-dihydro-2(lH)-quinolinone (515 mg) and trimethyloxonium tetrafluoroborate (590 mg) in 50 mL of dry pentene stabilized chloroform is stirred at room temperature overnight. To this mixture is then added l-(pyridin-2-yl)-4-(2- aminoethyl)piperazine (800 mg) and triethylamine (5 mL). The resultant solution is refluxed overnight under nitrogen. After cooling the solution is concentrated and partitioned between water and ethyl acetate. The organic layer is dried and concentrated to give a brown oil. Purification using preparative thin layer chromatography on silica eluting with 10% CH₃OH,^"

89 % CHCI₃, 1% NH₄OH provides the product as a colorless oil (610 mg, R_f = 0.51). This material is combined with 420 mg of fumaric acid in 5 mL of methanol. Isopropanol (20 mL) is added and the volume of solution reduced to approximately 5 mL on a hot plate. Upon cooling, the product (700 mg, m.p. 200-202 °C) is collected by filtration.

Example 4

Preparation of 2-3.4-dihvdroquinolyl(^'2-(4-(^'5-fluoropyrimidin-2-yl piperazinyl ethyl.amine (Compound 3 ^s)

A solution of 3,4-dihydro-2(lH)-quinolinone (515 mg) and trimethyloxonium tetrafluoroborate (590 mg) in 50 mL of dry pentene stabilized chloroform is stirred at room temperature overnight. To this mixture is then added l-(5-fluoropyrimidin-2-yl)-4-(2- aminoethyl)piperazine (650 mg) and triethylamine (5 mL). The resultant solution is refluxed overnight under nitrogen. After cooling the solution is concentrated and partitioned between water and ethyl acetate. The organic layer is dried and concentrated. Purification using preparative thin layer chromatography on silica eluting with 10% CH₃OH, 89 % CHCI₃, 1%

NH₄OH affords the product as a yellow oil (410 mg, R_f = 0.46). This material is combined

with 270 mg of fumaric acid in 5 mL of methanol. Isopropanol (20 mL) is added and the solution reduced to a volume of approximately 5 mL on a hot plate. Upon cooling, the product (349 mg, m.p. 170-171 °C) is collected by filtration.

Example 5

The following compounds are prepared essentially according to the procedures set forth above in Examples 1 and 2. (a) (2-(4-pyrimidin-2-ylpiperazinyl)ethyl)-2-quinolylamine hydrobromide (Compound 4, m.p. 174-176°C)

(b) (3-(4-pyrimidin-2-ylpiperazinyl)propyl)-2-quinolylamine hydrobromide

(Compound 5, m.p. 127-128 °C)

(c) (4-(4-pyrimidin-2-ylpiperazinyl)butyl)-2-quinolylamine hydrobromide (Compound 6, m.p. 274-276 °C)

(d) (3-(4-(2-methoxyphenyl)piperazinyl)propyl)-2-quinolylamine fumarate (Compound 7, m.p. 159-160 °C)

(e) (3 -(4-(2-pyridyl)piperazinyl)propyl)-2-quinolylamine hydrobromide (Compound 8, m.p. 137-139 °C)

(f) (3-(4-phenylpiperazinyl)propyl)-2-quinolylamine hydrobromide (Compound 9, m.p. 228-229 °C)

(g) (3-(4-(2,3-dimethylphenyl)piperazinyl)propyl)-2-quinolylamine fumarate

(Compound 10, m.p. 186-187 °C)

(h) (4-methyl(2-quinolyl))(2-(4-pyrimidin-2-ylpiperazinyl)ethyl)amine hydrobromide (Compound 11, m.p. >270 °C)

(i) (4-methyl(2-quinolyl))(3-(4-pyrimidin-2-ylpiperazinyl)propyl)amine hydrobromide (Compound 12, m.p. 255-260 °C) (j) (4-methyl(2-quinolyl))(4-(4-pyrimidin-2-ylpiperazinyl)butyl)amine hydrobromide (Compound 13, m.p. >260°C)

(k) (4-methyl(2-quinolyl))(3-(4-(2-methoxyphenyl)piperazinyl)propyl)amine fumarate (Compound 14, m.p. 177-179 °C)

(1) (4-methyl(2-quinolyl))(3-(4-(2-pyridyl)piperazinyl)propyl)amine hydrobromide (Compound 15)

(m) (4-methyl(2-quinolyl))(3-(4-phenylpiperazinyl)propyl)amine fumarate (Compound 16, m.p. 208-209 °C)

(n) (4-methyl(2-quinolyl))(3-(4-(2,3-dimethylphenyl)piperazinyl)propyl)amine fumarate (Compound 17, m.p 159-160 °C)

(o) (2-(4-(5-fluoropyrimidin-2-yl)piperazinyl)ethyl)-2-quinolylamine hydrobromide (Compound 18, m.p. 150-151 °C)

(p) (4-methyl(2-quinolyl))(2-(4-(5-fluoropyrimidin-2-yl)piperazinyl)ethyl)amine hydrobromide (Compound 19, m.p. 250-253 °C)

(q) (4-(4-pyrimidin-2-ylpiperazinyl)butyl)-2-quinolylamine (compound 20)

(r) (3-(4-(2,3-dimethylphenyl)piperazinyl)propyl)-2-quinolylamine (Compound

21) (s) (4-methyl(2-quinolyl))(3-(4-(pyrimidin-2-yl)piperazinyl)propyl)amine (Compound 22)

Example 6 The following compounds are prepared essentially according to the procedures set forth above in Examples 3 and 4.

(a) 2-3 ,4-dihydroquinolyl(2-(4-(pyrimidin-2-yl)piperazinyl)ethyl)amine fumarate (Compound 20, m.p. 217-218 °C)

(b) 2-3,4-dihydroquinolyl(3-(4-(pyrimidin-2-yl)piperazinyl)propyl)amine fumarate

(Compound 21, m.p. 194-195 °C)

(c) 2-3,4-dihydroquinolyl(4-(4-(5-fluoropyrimidin-2-yl)piperazinyl)butyl)amine hydrobromide (Compound 22, m.p. 279-280 °C, dec)

(d) 2-3,4-dihydroquinolyl(2-(4-(5-fluoropyrimidin-2-yl)piperazinyl)ethyl)amine fumarate (Compound 23, m.p. 200-201 °C)

(e) 2-3,4-dihydroquinolyl(3-(4-(5-fluoropyrimidin-2-yl)piperazinyl)propyl)amine fumarate (Compound 24, m.p. 173-176 °C)

(f) 2-3,4-dihydroquinolyl(4-(4-(5-fluoropyrimidin-2-yl)piperazinyl)butyl)amine fumarate (Compound 25, m.p. 182-183 °C)

(g) 2-3,4-dihydroquinolyl(4-(4-(5-methylpyrimidin-2-yl)piperazinyl)butyl)amine fumarate (Compound 26, m.p. 253-254 °C)

(h) 2-3 ,4-dihydroquinolyl(2-(4-(2-pyridyl)piperazinyl)ethyl)amine fumarate (Compound 27) (i) 2-3,4-dihydroquinolyl(3-(4-(2-pyridyl)piperazinyl)propyl)amine fumarate (Compound 28, m.p. 190-191 °C)

(j) 2-3,4-dihydroquinolyl(4-(4-(2-pyridyl)piperazinyl)butyl)amine fumarate

(Compound 29, 154-155 °C)

(k) l-(3-(2-3,4-dihydroquinolylamino)propyl)-4-phenylpiperidin-4-ol hydrobromide (Compound 30, m.p. 247 °C)

(1) 4-(4-chlorophenyl)-l-(3-(2-3,4-dihydroquinolylamino)propyl)piperidin-4-ol hydrobromide (Compound 31, m.p. >260 °C)

Example 7 The pharmaceutical utility of compounds of this invention is indicated by the assays for dopamine receptor subtype affinity described below.

Representative examples of 2-aminoalkylaminoquinolines according to the invention and representative corresponding biological acitvities are shown in Table 1 below. The number below each compound is its compound number. Each of these compounds may be prepared according to the reactions described above and shown in Scheme I. Compounds 1-4 in Table 1 have the following general formula A:

A where R₃ and R₇ are defined in the table.

Compounds 5-6 in Table 1 have the following general formula B:

B where R₃ and R₇ are defined in the table.

Assay for D? and D4 Receptor Binding Activity

Pellets of COS cells containing recombinantly produced D2 or D4 receptors from

African Green monkey were used for the assays. The sample is homogenized in 100 volumes (w/vol) of 0.05 M Tris HCI buffer at 4° C and pH 7.4. The sample is then centrifuged at 30,000 x g and resuspended and rehomogenized. The sample is then centrifuged as described and the final tissue sample is frozen until use. The tissue is resuspended 1 :20 (wt/vol) in 0.05 M Tris HCI buffer containing 100 mM NaCl.

Incubations are carried out at 48°C and contain 0.4 ml of tissue sample, 0.5 nM -1H-

YM 09151-2 and the compound of interest in a total incubation of 1.0 ml. Nonspecific binding is defined as that binding found in the presence of 1 mM spiperone; without further additions, nonspecific binding is less than 20% of total binding. Binding characteristics of various compounds of the invention for D2 and D4 receptor subtypes are shown in Table 1 fof

rat striatal homogenates.

Various compounds of the invention were also evaluated in M, and M₂ Muscarinic subtype assays essentially as set forth in U.S. Patent No. 5,093,333. The acitivity of the compounds is shown in Table 1.

Table 1

Comound D2 D₄ M, M₂ Number Ri R₇ Ki (nM) Ki (nM) (nM) (nM)

Various compounds disclosed in U.S. Patent No. 5,093,333 (Saab) were evaluated in the D₄, M_j, and M₂ receptor assays as described above. The activity of these compounds is shown below in Table 2. Saab Example Structure M, M, D₄ No.

The invention and the manner and process of making and using it, are now described in such full, clear, concise and exact terms as to enable any person skilled in the art to which it pertains, to make and use the same. It is to be understood that the foregoing describes preferred embodiments of the present invention and that modifications may be made therein without departing from the spirit or scope of the present invention as set forth in the claims. To particularly point out and distinctly claim the subject matter regarded as invention, the following claims conclude this specification.

Claims

What is claimed is:

A compounds of the formula:

or the pharmaceutically acceptable acid addition salts thereof wherein:

Ri, R₂, R₃, R4 and R₅ are the same or different and represent hydrogen, halogen, Cj-C₆ alkyl,

Cj-C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino, mono(C,-C₆) alkylamino, di(Cι-C₆) alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy; R₆ is hydrogen or Cι-C₆ alkyl;

W is nitrogen, COH, or CH;

Y and Z independently represent nitrogen or CH; and A represents an alkylene group of from 2-5 carbon atoms optionally substituted with one or more alkyl groups having from one to four carbon atoms.

A compound according to claim 1, wherein R₆ is hydrogen, methyl or ethyl.

A compound of the formula:

or the pharmaceutically acceptable acid addition salts thereof wherein:

C₁-C₄ alkoxy, C₁-C₄ alkylthio, hydroxy, amino, mono(C,-C₆) alkylamino, di(Cι-C₆) alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy; R is hydrogen or Cι-C₆ alkyl; W is nitrogen, COH, or CH;

4. A compound according to claim 3, wherein R₆ is hydrogen, methyl or ethyl.

5. A compound of the formula:

or the pharmaceutically acceptable acid addition salts thereof wherein: Ri, R2, R₃, R4 and R₅ are the same or different and represent hydrogen, halogen, Cι-C₆ alkyl, ^~ C₁-C4 alkoxy, C₁-C₄ alkylthio, hydroxy, amino, mono(C,-C₆) alkylamino, di(C]-C₆) alkylamino, cyano, nitro, trifluoromethyl or trifluoromethoxy;

Rβ is hydrogen or Cj- alkyl; W is nitrogen, COH, or CH;

Y and Z independently represent nitrogen or CH; and

6. A compound according to claim 3, wherein Rβ is hydrogen, methyl or ethyl.

7. A compound according to claim 1, which is (2-(4-pyrimidin-2- ylpiperazinyl)ethyl)-2-quinolylamine.

8. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(2-(4-(2- pyridyl)piperazinyl)ethyl)amine.

9. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(2-(4-(5- fluoropyrimidin-2-yl)piperazinyl)ethyl)amine.

10. A compound according to claim 1 , which is (2-(4-pyrimidin-2- ylpiperazinyl)ethyl)-2-quinolylamine hydrobromide.

11. A compound according to claim 1 , which is (3-(4-pyrimidin-2- ylpiperazinyl)propyl)-2-quinolylamine hydrobromide.

12. A compound according to claim 1, which is (4-(4-pyrimidin-2- ylpiperazinyl)butyl)-2-quinolylamine hydrobromide.

13. A compound according to claim 1, which is (3-(4-(2- methoxyphenyl)piperazinyl)propyl)-2-quinolylamine fumarate.

14. A compound according to claim 1, which is A compound according to claim 1, which is (3-(4-(2-pyridyl)piperazinyl)propyl)-2-quinolylamine hydrobromide.

15. A compound according to claim 1, which is (3-(4-phenylpiperazinyl)propyl)-2- quinolylamine hydrobromide.

16. A compound according to claim 1 , which is (3-(4-(2,3- dimethylphenyl)piperazinyl)propyl)-2-quinolylamine fumarate.

17. A compound according to claim 1, which is (4-methyl(2-quinolyl))(2-(4- pyrimidin-2-ylpiperazinyl)ethyl)amine hydrobromide.

18. A compound according to claim 1, which is (4-methyl(2-quinolyl))(3-(4- pyrimidin-2-ylpiperazinyl)propyl)amine hydrobromide.

19. A compound according to claim 1, which is (4-methyl(2-quinolyl))(4-(4- pyrimidin-2-ylpiperazinyl)butyl)amine hydrobromide.

20. A compound according to claim 1, which is (4-methyl(2-quinolyl))(3-(4-(2- methoxyphenyl)piperazinyl)propyl)amine fumarate.

21. A compound according to claim 1, which is (4-methyl(2-quinolyl))(3-(4-(2- pyridyl)piperazinyl)propyl)amine hydrobromide.

22. A compound according to claim 1, which is (4-methyl(2-quinolyl))(3-(4- phenylpiperazinyl)propyl)amine fumarate.

23. A compound according to claim 1, which is (4-methyl(2-quinolyl))(3-(4-(2,3- dimethylphenyl)piperazinyl)propyl)amine fumarate.

24. A compound according to claim 1, which is (2-(4-(5-fluoropyrimidin-2- yl)piperazinyl)ethyl)-2-quinolylamine hydrobromide.

25. A compound according to claim 1, which is (4-methyl(2-quinolyl))(2-(4-(5- fluoropyrimidin-2-yl)piperazinyl)ethyl)amine hydrobromide.

26. A compound according to claim 1, which is (4-(4-pyrimidin-2- ylpiperazinyl)butyl)-2-quinolylamine.

27. A compound according to claim 1, which is (3-(4-(2,3- dimethylphenyl)piperazinyl)propyl)-2-quinolylamine.

28. A compound according to claim 1, which is (4-methyl(2-quinolyl))(3-(4- (pyrimidin-2-yl)piperazinyl)propyl)amine.

29. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(2-(4-

(pyrimidin-2-yl)piperazinyl)ethyl)amine fumarate.

30. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(3-(4- (pyrimidin-2-yl)piperazinyl)propyl)amine fumarate.

31. A compound according to claim 1 , which is 2-3,4-dihydroquinolyl(4-(4-(5- fluoropyrimidin-2-yl)piperazinyl)butyl)amine hydrobromide.

32. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(2-(4-(5- fluoropyrimidin-2-yl)piperazinyl)ethyl)amine fumarate.

33. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(3-(4-(5- fluoropyrimidin-2-yl)piperazinyl)propyl)amine fumarate.

34. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(4-(4-(5- fluoropyrimidin-2-yl)piperazinyl)butyl)amine fumarate.

35. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(4-(4-(5- methylpyrimidin-2-yl)piperazinyl)butyl)amine fumarate.

36. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(2-(4-(2- pyridyl)piperazinyl)ethyl)amine fumarate.

37 A compound according to claim 1 , which is 2-3,4-dihydroquinolyl(3-(4-(2- pyridyl)piperazinyl)propyl)amine fumarate.

38. A compound according to claim 1, which is 2-3,4-dihydroquinolyl(4-(4-(2- pyridyl)piperazinyl)butyl)amine fumarate.

39. A compound according to claim 1, which is l-(3-(2-3,4- dihydroquinolylamino)propyl)-4-phenylpiperidin-4-ol hydrobromide.

40. A compound according to claim 1, which is 4-(4-chlorophenyl)-l-(3-(2-3,4- dihydroquinolylamino)propyl)piperidin-4-ol hydrobromide.