WO1998051785A1 - Co-culture de cellules dans une configuration micromodelisee - Google Patents
Co-culture de cellules dans une configuration micromodelisee Download PDFInfo
- Publication number
- WO1998051785A1 WO1998051785A1 PCT/US1997/008254 US9708254W WO9851785A1 WO 1998051785 A1 WO1998051785 A1 WO 1998051785A1 US 9708254 W US9708254 W US 9708254W WO 9851785 A1 WO9851785 A1 WO 9851785A1
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- WO
- WIPO (PCT)
- Prior art keywords
- cells
- cell
- cell type
- culture
- hepatocytes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2535/00—Supports or coatings for cell culture characterised by topography
- C12N2535/10—Patterned coating
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
L'invention concerne des procédés de production de co-cultures de cellules dont au moins deux types sont configurés dans un micromodèle sur un substrat de façon que la modulation de l'étendue des contacts cellules-cellules hétérotypiques puisse être utilisée pour moduler des fonctions métaboliques et/ou synthétiques des cellules. On peut mettre en oeuvre la co-culture en revêtant une protéine telle que le collagène sur un substrat de façon que la protéine définisse un micromodèle sur le substrat, en mettant en contact le substrat revêtu avec des cellules d'un premier type telles que les hépatocytes dans un milieu afin de lier les cellules avec la protéine dans le but de produire un substrat revêtu de cellules micromodélisées et en mettant en contact le substrat micromodélisé avec les cellules d'un second type telles que les fibroblastes dans un second milieu afin de lier les cellules au substrat. Les deux milieux sont pour l'un, un milieu de non fixation, tel qu'un milieu exempt de sérum, et pour l'autre, un milieu de fixation tel qu'un milieu renfermant du sérum. Le milieu de fixation peut contenir un facteur de fixation tel que la fibronectine ou un anticorps. Le micromodèle peut être formé d'îlots de cellules du premier ou second type entourés de cellules de l'autre type.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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PCT/US1997/008254 WO1998051785A1 (fr) | 1997-05-14 | 1997-05-14 | Co-culture de cellules dans une configuration micromodelisee |
JP54917598A JP2002510969A (ja) | 1997-05-14 | 1997-05-14 | マイクロパターン化形態における細胞の共培養 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1997/008254 WO1998051785A1 (fr) | 1997-05-14 | 1997-05-14 | Co-culture de cellules dans une configuration micromodelisee |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998051785A1 true WO1998051785A1 (fr) | 1998-11-19 |
Family
ID=22260905
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/008254 WO1998051785A1 (fr) | 1997-05-14 | 1997-05-14 | Co-culture de cellules dans une configuration micromodelisee |
Country Status (2)
Country | Link |
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JP (1) | JP2002510969A (fr) |
WO (1) | WO1998051785A1 (fr) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1428871A1 (fr) * | 2001-07-26 | 2004-06-16 | Kazunori Kataoka | Construction cellulaire cultivee contenant des spheroides de cellules animales cultivees et utilisation correspondante |
JP2007244875A (ja) * | 1999-02-01 | 2007-09-27 | Orthogen Ag | 体液から治療上有効なタンパク質であるインターロイキン−1受容体拮抗薬を産生するための方法 |
EP1904625A2 (fr) * | 2005-05-24 | 2008-04-02 | The Regents of the University of California | Tissus in vitro élaborés, microstructurés et à micro-échelle |
US7687251B2 (en) | 2004-03-26 | 2010-03-30 | Dai Nippon Printing Co., Ltd. | Method for producing cell culture substrate and apparatus for producing cell culture substrate |
US7919305B2 (en) | 2004-02-19 | 2011-04-05 | Dai Nippon Printing Co., Ltd. | Method for manufacturing cell culture substrate |
WO2012027218A2 (fr) | 2010-08-27 | 2012-03-01 | Corning Incorporated | Surfaces modifiées par un peptide pour culture cellulaire |
AU2012216645B2 (en) * | 2005-05-24 | 2015-06-25 | The Regents Of The University Of California | Microscale micropatterned engineered in vitro tissue |
US9249386B2 (en) | 2005-06-06 | 2016-02-02 | Dai Nippon Printing Co., Ltd. | Substrate for cell transfer |
US10266806B2 (en) * | 2014-01-14 | 2019-04-23 | Colorado State University Research Foundation | Stem cell-derived hepatocytes in co-culture and uses thereof |
US11407979B2 (en) | 2017-09-01 | 2022-08-09 | Colorado State University Research Foundation | Intermittent starvation techniques to prolong functional lifetime of liver cells in culture |
US11608486B2 (en) | 2015-07-02 | 2023-03-21 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
US11613727B2 (en) | 2010-10-08 | 2023-03-28 | Terumo Bct, Inc. | Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
US11629332B2 (en) | 2017-03-31 | 2023-04-18 | Terumo Bct, Inc. | Cell expansion |
US11634677B2 (en) | 2016-06-07 | 2023-04-25 | Terumo Bct, Inc. | Coating a bioreactor in a cell expansion system |
US11667876B2 (en) | 2013-11-16 | 2023-06-06 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
US11667881B2 (en) | 2014-09-26 | 2023-06-06 | Terumo Bct, Inc. | Scheduled feed |
US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
US11795432B2 (en) | 2014-03-25 | 2023-10-24 | Terumo Bct, Inc. | Passive replacement of media |
US11965175B2 (en) | 2016-05-25 | 2024-04-23 | Terumo Bct, Inc. | Cell expansion |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6943021B2 (en) * | 2002-06-07 | 2005-09-13 | Mattek Corporation | Three dimensional vaginal tissue model containing immune cells |
JP4565916B2 (ja) * | 2004-07-23 | 2010-10-20 | 三洋電機株式会社 | 光反応装置 |
Citations (2)
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US4614517A (en) * | 1982-08-04 | 1986-09-30 | La Jolla Cancer Research Foundation | Tetrapeptide |
US5602029A (en) * | 1994-06-27 | 1997-02-11 | Nec Corporation | Method for fabricating substrate for cell culture and method for cell arrangements |
-
1997
- 1997-05-14 WO PCT/US1997/008254 patent/WO1998051785A1/fr active Search and Examination
- 1997-05-14 JP JP54917598A patent/JP2002510969A/ja active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4614517A (en) * | 1982-08-04 | 1986-09-30 | La Jolla Cancer Research Foundation | Tetrapeptide |
US5602029A (en) * | 1994-06-27 | 1997-02-11 | Nec Corporation | Method for fabricating substrate for cell culture and method for cell arrangements |
Non-Patent Citations (2)
Title |
---|
BHATIA et al., "Controlling Cell Interactions by Micropatterning in Co-Cultures: Hepatocytes and 3T3 Fibroblasts", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, February 1997, Vol. 34, No. 2, pages 189-199. * |
IRELAND et al., "Effect of Patterned Surfaces of Adhesive Islands on the Shape, Cytoskeleton, Adhesion and Behaviour of Swiss Mouse 3T3 Fibroblasts", JOURNAL OF CELL SCIENCE SUPPLEMENT, 1987, Vol. 8, pages 19-33. * |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007244875A (ja) * | 1999-02-01 | 2007-09-27 | Orthogen Ag | 体液から治療上有効なタンパク質であるインターロイキン−1受容体拮抗薬を産生するための方法 |
EP1428871A4 (fr) * | 2001-07-26 | 2004-11-24 | Kazunori Kataoka | Construction cellulaire cultivee contenant des spheroides de cellules animales cultivees et utilisation correspondante |
EP1428871A1 (fr) * | 2001-07-26 | 2004-06-16 | Kazunori Kataoka | Construction cellulaire cultivee contenant des spheroides de cellules animales cultivees et utilisation correspondante |
US7919305B2 (en) | 2004-02-19 | 2011-04-05 | Dai Nippon Printing Co., Ltd. | Method for manufacturing cell culture substrate |
US8497117B2 (en) | 2004-02-19 | 2013-07-30 | Dai Nippon Printing Co., Ltd. | Method for manufacturing cell culture substrate |
US7687251B2 (en) | 2004-03-26 | 2010-03-30 | Dai Nippon Printing Co., Ltd. | Method for producing cell culture substrate and apparatus for producing cell culture substrate |
AU2006250079B2 (en) * | 2005-05-24 | 2012-05-31 | The Regents Of The University Of California | Microscale micropatterned engineered in vitor tissue |
EP2495310A3 (fr) * | 2005-05-24 | 2013-03-27 | The Regents Of the University of California | Microfiltration à micromotifs créée dans des tissus in vitro |
EP1904625A4 (fr) * | 2005-05-24 | 2009-12-23 | Univ California | Tissus in vitro élaborés, microstructurés et à micro-échelle |
AU2012216645B2 (en) * | 2005-05-24 | 2015-06-25 | The Regents Of The University Of California | Microscale micropatterned engineered in vitro tissue |
EP1904625A2 (fr) * | 2005-05-24 | 2008-04-02 | The Regents of the University of California | Tissus in vitro élaborés, microstructurés et à micro-échelle |
US9249386B2 (en) | 2005-06-06 | 2016-02-02 | Dai Nippon Printing Co., Ltd. | Substrate for cell transfer |
WO2012027218A2 (fr) | 2010-08-27 | 2012-03-01 | Corning Incorporated | Surfaces modifiées par un peptide pour culture cellulaire |
US11613727B2 (en) | 2010-10-08 | 2023-03-28 | Terumo Bct, Inc. | Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
US11773363B2 (en) | 2010-10-08 | 2023-10-03 | Terumo Bct, Inc. | Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
US11746319B2 (en) | 2010-10-08 | 2023-09-05 | Terumo Bct, Inc. | Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
US11667876B2 (en) | 2013-11-16 | 2023-06-06 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
US11708554B2 (en) | 2013-11-16 | 2023-07-25 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
US10266806B2 (en) * | 2014-01-14 | 2019-04-23 | Colorado State University Research Foundation | Stem cell-derived hepatocytes in co-culture and uses thereof |
US10717966B2 (en) | 2014-01-14 | 2020-07-21 | Colorado State University Research Foundation | Stem cell-derived hepatocytes in co-culture and uses thereof |
US11795432B2 (en) | 2014-03-25 | 2023-10-24 | Terumo Bct, Inc. | Passive replacement of media |
US11667881B2 (en) | 2014-09-26 | 2023-06-06 | Terumo Bct, Inc. | Scheduled feed |
US11608486B2 (en) | 2015-07-02 | 2023-03-21 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
US11965175B2 (en) | 2016-05-25 | 2024-04-23 | Terumo Bct, Inc. | Cell expansion |
US11634677B2 (en) | 2016-06-07 | 2023-04-25 | Terumo Bct, Inc. | Coating a bioreactor in a cell expansion system |
US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
US11702634B2 (en) | 2017-03-31 | 2023-07-18 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
US11629332B2 (en) | 2017-03-31 | 2023-04-18 | Terumo Bct, Inc. | Cell expansion |
US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
US11407979B2 (en) | 2017-09-01 | 2022-08-09 | Colorado State University Research Foundation | Intermittent starvation techniques to prolong functional lifetime of liver cells in culture |
Also Published As
Publication number | Publication date |
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JP2002510969A (ja) | 2002-04-09 |
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