WO1998048767A1

WO1998048767A1 - Humectant, and cosmetic preparation and medicine both containing the same

Info

Publication number: WO1998048767A1
Application number: PCT/JP1998/001929
Authority: WO
Inventors: Yoshio Furusawa
Original assignee: Teikoku Hormone Mfg. Co., Ltd.
Priority date: 1997-04-28
Filing date: 1998-04-27
Publication date: 1998-11-05
Also published as: JP4424759B2

Abstract

A humectant containing as the active ingredient a fraction of the parotid secretion of a mammal which precipitates from a water-miscible organic solvent at a concentration of 60 % (v/v) or higher; a method of moisturizing the skin, hair, and scalp by applying this humectant thereto; a cosmetic preparation comprising a cosmetic base and the above fraction; and a medicine comprising a base for medicinal external preparations and the above fraction. The humectant fraction derived from the parotid secretion of a mammal, used in the humectant, cosmetic preparation, and medicine, can perform its moisturizing function even in an extremely small amount to not only enhance the moisture-retaining function of the skin but improve the slip properties of the hair. Furthermore, it is safe for the human body.

Description

Description-Moisturizer, cosmetics and pharmaceuticals containing it

TECHNICAL FIELD The present invention relates to a novel humectant, a humidifying method using the humectant, and cosmetics and pharmaceuticals containing the humectant. More specifically, the present invention is a novel moisturizer that has excellent moisturizing properties, has high durability of moisturizing function, can impart moist feeling and smoothness to skin and hair, and is safe for the human body. The present invention relates to a method for moisturizing skin and hair using the humectant, and to cosmetics and pharmaceuticals containing the humectant. Skill

Skin cosmetics are generally prepared by blending a hydrophilic skin moisturizer and an oily emollient to give the skin adequate moisture and oil to prevent the skin from drying out. You.

Conventionally, polyethers such as polyethylene glycol and polypropylene glycol, glycerin, and sorbitol have been used as the hydrophilic skin moisturizer, but in recent years, natural moisturizing factor (NMF) has been used. Amino acids, sodium pyrrolidonecarboxylate, urea, sodium lactate, etc., and natural high molecular weight compounds such as sodium hyaluronate and salivary gland mucin have been used.

However, conventionally used moisturizers such as polyethers, glycerin, and sorbitol have the drawback of being dermatologically heterogeneous, and also have the disadvantage of removing water from the outermost layer of the skin, the stratum corneum. NMFs may be absorbed and rather cause skin to lose moisture.NMFs, which have been used in recent years, are substances derived from natural products, but they have a poor feeling on use and have a poor moisturizing effect. There are drawbacks such as On the other hand, sodium hyaluronate is currently used in many cosmetics and gives a skin a "moist feeling" and "smoothness" in a concentration range of about 0.02 to 0.1% by weight. Its usefulness has been highly evaluated. In addition, for safety testing In the range of 2 to 3% by weight, no local irritation or sensitization was observed in laboratory animals, and in human patch tests, it was safe even at a concentration of 1% by weight. Has been confirmed.

Salivary gland mucins also have an excellent moisturizing effect, and their "moistness" and "smoothness" on the skin are evaluated as being superior to sodium hyaluronate. It has been confirmed that salivary gland mucin has moisturizing and emulsifying effects and is highly safe for the human body, and can be said to be a fairly ideal moisturizer. Salivary gland mucin is a glycoprotein that is biosynthesized and secreted by the salivary glands of mammals, for example, pests, pomas, bushes, and sheep, such as the submandibular, parotid, and sublingual glands. It has an inherent viscosity depending on the type of animal and is obtained as a macromolecule containing a large amount of sialic acid and having a molecular weight of about 100,000 to 100,000.

Focusing on the excellent properties of such natural polymer compounds, in recent years, as a moisturizer for cosmetics, pharmaceuticals, etc. (especially skin external preparations), and in various industrial fields such as foods, cosmetics, pharmaceuticals, quasi-drugs, and toiletries , Research on its use as an emulsifier has been promoted and some have been put into practical use. However, it is said that the humectant composed of these natural polymer compounds requires too much production cost to be used in an amount sufficient to exhibit its performance, and that its effect in hair cosmetics cannot be expected. There are drawbacks. In addition, cosmetics containing sodium hyaluronate and salivary gland mucin improve the feeling of use, but because both components are easily decomposed, the molecular weight decreases over time, and the moisturizing property and viscosity decrease. Another disadvantage is that the moisturizing film is easily removed by washing with water or hot water.

On the other hand, liquid oils such as liquid paraffin and petrolatum are used as emollients, but when these emollients are added to such an extent that they prevent normal evaporation of water from the epidermis, unpleasant feeling due to stickiness And lacks usefulness as a skin moisturizer. Disclosure of the invention

Under such circumstances, the first object of the present invention is to (1) exert a moisturizing property in an extremely small amount, and can be blended into various cosmetic bases and bases for external preparations for pharmaceuticals. ) Skin and (3) It can give a moist feeling and "smoothness" to hair and hair. (3) It does not give a feeling of incongruity such as "stickiness" or "sharpness" like conventional oily emollients. (4) Moisturizing. (5) The moisturizing film is not removed even after washing with water or hot water several times, (6) It is highly safe for the human body. Is to provide a suitable humectant.

A second object is to provide a method for moisturizing skin and hair using the humectant, and a third object is to provide cosmetics and medicines containing the humectant. It is to be.

The present inventors have conducted intensive studies to achieve the above object, and as a result, many animals lick their bodies with their tongues and groom, and when saliva dries, beautiful and smooth shiny hair is obtained. Focusing on the fact that the wound is moisturized with the tongue, preventing dryness and accelerating the healing of the wound, a certain method is used from the parotid gland secretion of mammals. The separated components are (a) extremely moist, and exhibit a moisturizing function, and are safe for the human body. (B) Even in powder form, they dissolve in water and apply to the skin to exert a moisturizing function. (C) It can be used by blending it with various cosmetic bases and bases for external preparations for medicines. Lotion, base for external medicines, etc. It has the property of enhancing the function of retaining moisture. (D) It has excellent adsorptivity to the skin and gives the skin and hair a "moist feeling" and "smoothness", while "sticky" and "shaky" (E) It does not absorb the moisture from the stratum corneum of the skin more than necessary, and lacks the points like a conventional hydrophilic moisturizer. (F) Withstands several times of washing with water or hot water, does not remove the moisturizing film on the skin surface even after washing, and does not change the moisture retention state of the skin. The present invention was completed based on this finding.

That is, the present invention

(1) A moisturizer characterized by containing, as an active ingredient, a fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid secretion of mammals. Agent,

(2) A method for moisturizing the skin, comprising applying the humectant of (1) to the skin, (3) A moisturizing method for hair and scalp, which comprises applying the moisturizing agent of (1) to the hair or scalp or both.

(4) A cosmetic comprising a cosmetic base and a fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid gland secretion of a mammal. ,

and

(5) As a component of the pharmaceutical external preparation base, a fraction precipitated at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid gland secretion of mammals should be contained. Pharmaceutical products,

Is provided. BEST MODE FOR CARRYING OUT THE INVENTION

The humectant of the present invention contains, as an active ingredient, a substance derived from the parotid fluid of a mammal, and the mammal is not particularly limited. Although it is preferable to take the secretion fluid into consideration, such as ease of collection, amount of secretion (1 to 50 to 60 liters is secreted in one case), economy, etc. .

Saliva secreted into the mouth of mammals (secreted fluid flowing into the mouth) is a mixture of secretions from the parotid, submandibular and sublingual glands. The ratio of salivary gland secretion to its secretion is about 70% by weight of parotid gland, 5% by weight of submandibular gland, and 25% by weight of sublingual gland. The secretion of the mammal used in the present invention is limited to that secreted from the parotid gland, and is substantially free of the secretion secreted from other salivary glands, the submandibular gland and the sublingual gland Things. Saliva as a mixture contains mucous and serous components, but the parotid gland secretion, which is the secretion from the parotid gland, is known to be serous and mostly water. ing. To date, only "all saliva" as a mixture has been studied for "saliva", and little has been done on the secretions of the three glands described above. The present inventors have studied the secretions obtained from the salivary glands and found out that the parotid secretions contained a component having an extremely excellent moisturizing function. To collect the parotid gland secretion of a mammal, an incision is made on the skin below the animal's parotid and the oral surface Expose the parotid gland duct near the skin and make a small incision in the parotid gland duct so that the tip of the forcenula can be inserted. Guide the force through the incision in the skin, and insert the tip of the force neuron from the incision in the parotid duct toward the parotid gland. The incision wound on the skin is closed while the cannula is fixed with adhesive tape. A receiver can be placed on the opposite end of the force neuron to collect the parotid gland fluid that flows off naturally.

When storing the collected parotid secretions, it is better to add preservatives and store them in a cool place. Here, the preservatives to be added include ordinary preservatives, for example, benzoic acids, paraoxybenzoic acids, dehydroacetic acids, sorbic acids, phenols, alcohols, sodium, rogens, glutaraldehydes,? -Probio Lactones and the like, or a mixture thereof. Preferably, it is odorless or one which can easily be removed in the subsequent step even if it has an odor, and paraoxy-benzoic acids are particularly preferred.

The humectant of the present invention is obtained by mixing a mammalian parotid gland secretion with a water-miscible organic solvent, and a fraction that precipitates at an organic solvent concentration of 60% (v / v) or more (hereinafter, the humectant fraction). ) May be contained as an active ingredient. The reason for setting the organic solvent concentration to 60% (v / v) or more is as follows. As the organic solvent is added to the parotid gland secretion, the solubility of the moisturizing components in the parotid gland secretion decreases and sedimentation begins to occur. The effective amount of organic solvent added is defined as the amount at which this precipitate can be substantially recovered, which is about 60% (v / v) or more. Therefore, the amount of addition varies slightly depending on the type of organic solvent, but in the case of the present invention, if the amount of addition of the organic solvent is less than 60% (v / v), practical precipitation cannot be expected. .

As described above, the concentration of the organic solvent is not particularly limited as long as it is 60% (v / v) or more, but considering the amount of formed precipitate and the economics of using an excessive amount of the organic solvent, The concentration of the organic solvent is preferably about 63 to 80% (v / v), and more preferably about 65 to 75% (v / v).

The parotid gland secretion collected as described above, or after filtration, is put into an extractor, and the water-miscible organic solvent is stirred preferably under cooling (about 0 to 15 ° C). In addition, collect the fraction that precipitates when the organic solvent concentration is 60% (v / v) or more. The obtained moisturizing fraction may be used as it is, but it is dehydrated and dried to obtain a powder. It is advantageous to use it. As a method of dehydration, there is a method of further adding a water-miscible organic solvent to the obtained fraction. As a drying method, there are an aeration method, a decompression method, and the like. These methods can be combined, and further heating can be applied.

Examples of the water-miscible organic solvent used here include acetone, ethanol, methanol, and isopropanol. These may be used alone or in combination of two or more. Is particularly preferred.

The yield of the moisturizing fraction depends on the health of the animal from which the parotid gland secretion is collected, but is generally 0.1% as a dry fraction relative to the parotid gland secretion. It is about 5 to 0.7% by weight. The nitrogen content and protein content in the dry and moisturizing fraction depend on animal health and the like, but are generally in the range of 0.05 to 0.4% by weight. And 0.4 to 3% by weight.

The substance having a moisturizing function of the moisturizing fraction has not yet been identified and is not necessarily a single substance. However, based on the nitrogen content and protein content, mucopolysaccharides such as hyaluronic acid and salivary gland mucin It is considered to be a substance different from the above.

Thus, the moisturizing fraction separated from the parotid gland secretion of the mammal has the following properties.

(1) It can give the skin and hair a feeling of firmness and smoothness without giving any unpleasant discomfort such as "stickiness" and "sharpness".

(2) It activates the action of skin cells, increases the flexibility and elasticity of the skin, keeps the skin healthy and gives the skin a youthful and shiny look.

(3) Even when powdered or simply dissolved in water, it exerts a moisturizing function on the skin, but when it is added to cosmetics or external preparations for pharmaceuticals, the synergistic effect with these functions further enhances moisturizing. Functions can be demonstrated.

(4) Since the moisturizing function is highly persistent, there is no need to apply it repeatedly over and over again a day as with conventional cosmetics.

(5) Even after washing with water or hot water several times, the moisturizing film on the skin does not fall off, and the excellent moisturizing property does not decrease.

(6) Even if used in large quantities, there is no danger of modulating physiological functions and it is extremely safe is there.

(7) It has good miscibility with various components commonly used in cosmetics and external preparations for pharmaceuticals, and there are no particular restrictions on the components to be mixed.

Although the humectant of the present invention contains the humectant fraction as an active ingredient, the humectant may contain a conventionally known humectant component, if desired. Known moisturizing components include, for example, polyethers such as polyethylene glycol and polypropylene glycol, polyhydric alcohols such as glycerin, 1,3-butylene glycol, propylene glycol, and sorbitol, and NMFs. Specific examples thereof include -amino acids, urea, sodium lactate, sodium borolidone carboxylate, mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, and proteins such as collagen and elastin.

Next, the moisturizing method of the present invention will be described. The moisturizing method of the present invention has two aspects. One is a method of applying the moisturizing agent of the present invention to the skin to moisturize the skin, whereby the skin has a “moist feeling” and “smoothness”. Is given.

The other is a method of applying the humectant of the present invention to the hair and / or scalp and moisturizing them, whereby the surface condition of the hair is improved and the slip on the hair surface (so-called comb) As expected), hair dressing is improved and drying of the scalp is improved, and itching and dandruff are suppressed.

In these methods, the form of the humectant at the time of application is not particularly limited, and for example, a humectant can be used as a cosmetic in various forms described below or as an external preparation in various forms.

Next, the cosmetics and pharmaceuticals of the present invention will be described.

First, the cosmetic of the present invention contains a cosmetic base and the moisturizing fraction separated from the above-mentioned mammalian parotid secretion.

The amount of the moisturizing fraction (dry state) contained in the cosmetic of the present invention depends on the form of the cosmetic and the presence or absence of other moisturizing components, but is usually 0.0 based on the total amount of the cosmetic. 0 to 2% by weight. If the content is less than 0.001% by weight, the moisturizing function may not be sufficiently exhibited, and if it exceeds 2% by weight, no improvement in the effect is recognized for the amount, and it is economically disadvantageous. Becomes Consider the moisturizing effect and economy The preferred content is in the range of 0.05 to 0.2% by weight. -The base used in the cosmetic of the present invention is not particularly limited, and is appropriately selected according to the desired form of the cosmetic from various components conventionally used as the base in cosmetics of various forms. You can choose.

Although the form of the cosmetic of the present invention is not particularly limited, for example, skin cosmetics such as lotion, milky lotion, creams, packs, lotions, lipsticks, foundations, skin cleansers, etc., hair conditioner, hair rinse, Hair cosmetics such as hair tonics, hair creams, and hair lotions can be mentioned, and the moisturizing fraction can be blended whether the base is aqueous or oily.

When the moisturizing fraction according to the present invention is blended with the skin cosmetic base, a more excellent moisturizing function is exerted due to a synergistic effect with the moisturizing function originally possessed by the cosmetic base. Can be.

In addition, a hair cosmetic in which the moisturizing fraction is blended with a hair cosmetic base has an excellent effect of keeping the hair and the scalp healthy. For example, when applied to the hair and the scalp after washing the hair, the hair By improving the surface condition of the hair, the slip on the hair surface (so-called comb-like) is improved and the riding of hair cosmetics is improved. It can also improve scalp drying and reduce itching and dandruff.

The cosmetic of the present invention may contain, if desired, various components conventionally used in cosmetics, for example, oils, surfactants, emulsifiers, alcohols, other moisturizing components, thickeners, preservatives, and antibacterial protection. Can contain fungicides, antioxidants, chelating agents, pH adjusters, ultraviolet absorbers, ultraviolet scattering agents, pigments, dyes, fragrances, vitamins, amino acids, water, etc.

Examples of the oil component include liquid oils such as castor oil, olive oil, jojoba oil, camellia oil, solid oils such as hardened castor oil, lanolin, whale wax, beeswax, carnauba wax, waxes such as candelilla wax, squalane, Examples include hydrocarbons such as petrolatum, liquid paraffin, sericin, and paraffin.

Examples of the surfactant include POE sorbin ester such as polyoxyethylene (POE) sorbin monooleate, sorbin ester such as sorbitan monooleate, P 0 E—P 0 E such as glyceryl monooleate. —Glycery Glycerin fatty acid esters such as glycerin fatty acid esters and glycerin monooleate;

POE—Polyesters such as monooleate; POE—alkyl ethers such as lauryl ether; POE—branched alkyl ethers such as POE—octyldodecyl ether; POE—branched alkyl ethers such as POE—nonylphenyl ether P 0 E—glycerol esters such as alkylphenyl ether and glycerol monoisostearate; POE such as POE—glycerol monoisostearate—polyglycerin fatty acid esters such as diglyceryl monostearate Nonionic surfactants, fatty acid soaps such as sodium and sodium salts of higher fatty acids such as stearic acid, higher alkyl sulfates such as sodium lauryl sulfate, and alkyls such as POE-triethanolamine lauryl sulfate Ether sulfate, LA N-acyl sarcosine salts such as roylsarcosine sodium, higher fatty acid amide sulfonates such as N-myristoyl-N-methyltaurine sodium, sodium alkylbenzenesulfonate such as sodium linear dodecylbenzenesulfonate, N-stearoyl Anionic surfactants such as N-acyl glutamate such as glutamate dinadium, alkylamine salts, POE-alkylamine salts, polyamine fatty acid derivatives, alkyl viridinium salts, alkyl quaternary ammonium salts, alkyldimethylbenzyl Cationic surfactants such as ammonium salts, alkyl isoquinolinium salts, dialkyl morpholinium salts, and benzenetonium chloride; and amphoteric surfactants such as benzoin-based, imidazoline-based, and amine oxide-based surfactants. It is below.

Examples of alcohols include lower alcohols such as ethyl alcohol, propyl alcohol, and isopropyl alcohol, glycerin, polyglycerin, trimethylolpropane, pentaerythritol, dipentyl erythritol, ethylene glycol, propylene glycol, polypropylene glycol, polypropylene glycol, and the like. 1,3-butylene glycol, 1,4-butylene glycol, and glucose, maltose, mannose, lactose, D-glucuronic acid, peronic acid, saccharose, D-mannite, D-sorbitol, sorbitan, glucono Monosaccharides such as lactone, cellulose, starch, arbutin, glucose phosphate and the like, polysaccharides and derivatives thereof are exemplified. As the other moisturizing components, the same components as those exemplified as the desired components in the description of the humectant of the present invention can be given.

Examples of the thickening agent include natural polymer substances such as sodium alginate, xanthan gum, malomelo seed extract, tragacanth gum, starch, and semi-synthetic polymers such as methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, soluble starch, and cationized cellulose. Substances, synthetic high molecular substances such as carboxyvinyl polymer and polyvinyl alcohol.

Preservatives Examples of the antibacterial and antifungal agents include methyl p-oxybenzoate, ethyl p-oxybenzoate, dehydroacetic acid, salicylic acid, benzoic acid, sorbic acid, and benzalkonium chloride. Examples thereof include dibutylhydroxytoluene, butylhydroxyanitool, provyl gallate, and ascorbic acid.

Examples of the chelating agent include disodium edetate, ethanehydroxydiphosphate, pyrophosphate, hexametaphosphate, citrate, tartaric acid, and gluconic acid, and examples of the pH adjuster include sodium hydroxide, Triethanolamine, cunic acid, sodium citrate, boric acid, borax, sodium monohydrogen phosphate and the like.

Further, examples of the ultraviolet absorber include 2-hydroxy-4-methoxybenzophenone, octyldimethyl p-aminobenzoate, and ethylhexyl p-methoxycinnamate, and examples of the ultraviolet scattering agent include titanium oxide, kaolin, and talc. No.

Examples of vitamins include, for example, vitamin A, vitamin B, vitamin C, biyumin D, vitamin E, vitamin F, vitamin, vitamin P, vitamin U, power lunitine, ferulic acid, hi-orizanol, herlipoic acid, Orotic acid and its derivatives include amino acids such as glycine, alanine, norin, oral isin, isoleucine, serine, threonine, fenylalanine, tyrosine, triptophan, cystine, cystine, methionine, proline, and hydroxy. Examples include proline, aspartic acid, glutamic acid, arginine, histidine, lysine and derivatives thereof. On the other hand, the medicament of the present invention contains the moisturizing fraction separated from the parotid gland secretion of the mammal as one component of the medicinal external preparation base.

The drug of the present invention can improve the function of retaining water on the skin surface by applying it to the skin as an external preparation. Conventionally, urea-based compounds, which are moisturizing components added to external preparations for pharmaceuticals, may cause skin crispness, pain, erythema, itching, burning, desquamation, hypersensitivity, etc. It can also occasionally cause eczema, skin cracks, rarely swelling and dryness.

The medicament of the present invention does not have the drawbacks of the above-mentioned urea compounds, and is effective for improving cracks, scabs and other diseases caused by dry skin, rough skin and dry skin. In addition, it improves the water retention function of the skin surface, which is advantageous in bringing out the therapeutic effect of the drug.

The amount of the moisturizing fraction (dry state) according to the present invention contained in the pharmaceutical of the present invention depends on the purpose of use, dosage form, presence or absence of other moisturizing components, and the like. It is in the range of 0.001-2% by weight based on the total amount of the chemical. If the content is less than 0.001% by weight, the moisturizing function may not be sufficiently exerted. If the content exceeds 2% by weight, no improvement in the effect is recognized for the amount, and economical efficiency is obtained. Disadvantaged. In consideration of the moisturizing effect and economy, the preferred content is in the range of 0.05 to 0.2% by weight.

There is no particular limitation on the base used in the pharmaceutical product of the present invention, and it can be appropriately selected according to the desired dosage form of the pharmaceutical from the various components conventionally used as the base in external preparations of various dosage forms. You can choose.

The dosage form of the drug of the present invention is not particularly limited, and includes, for example, creams, ointments, mouthwashes, etc., regardless of whether the base is hydrophilic or lipophilic. The moisturizing fraction according to the present invention can be blended.

The drug that can be used in the pharmaceutical of the present invention is not particularly limited, and any drug that can be administered in the form of an external preparation can be used. In addition, the medicine may contain, as desired, for example, each of the components exemplified as the desired components in the description of the cosmetic, an anti-inflammatory agent, an antipruritic agent, a bactericidal disinfectant, a feel improving agent, a hormonal agent and the like. Can be done. Hereinafter, the present invention will be described in more detail with reference to Production Examples, Test Examples, and Formulation Examples, but the present invention is not limited thereto.

Production Example 1

The parotid gland secretion was collected, and 0.15% by weight of methyl paraoxybenzoate was added as a preservative and stored in a cool place. The parotid gland secretion was filtered through filter paper No. 2 (Too Roshi Kaisha, Ltd.), and 100 liters thereof were stirred at 5 to 10 ° C in an extractor with a cooling device. While adding twice the amount (v / v) of acetone (acetone concentration: 66.6% (v / v)), a precipitate was formed, and the precipitate was collected. The resulting precipitate was dehydrated by adding an appropriate amount of acetone, and the acetone was removed by filtration to obtain an acetone-dehydrated cake. It was ground, air dried further dried under reduced pressure at 60 ^e C from to give © sheet parotid derived moisturizing fraction 436 g (yield: 0.44%). The nitrogen content of the obtained moisturizing fraction was 0.13% by weight, and the protein content was 1.06% by weight.

Test Example 1: Skin effect test A (sensory test)

In order to evaluate the skin aging prevention effect of the moisturizing fraction obtained in Production Example 1, a beautiful skin effect test (sensory test) was performed in the following manner.

Add 1100 milliliters of water to 2 Omg of the moisturizing fraction, dissolve it, and uniformly infiltrate it into 200 g of `` absorbent cotton '' (made by EF3 Co., Ltd. This was heated and dried to obtain a test sample. The content of the moisturizing fraction was 0.01% by weight based on the weight of absorbent cotton.

Next, the effect of applying this sample to 10 middle-aged (aged 35 to 62) subjects washed with warm water until their fingers and hands became “bulky” was examined. The back of the test subject's hand and the finger / palm test site were rubbed lightly and sufficiently with 0.5 g of the cut cotton, and the skin feeling after that was answered. After application, hands were free to be washed.

The test results were as follows: 8 subjects answered that the skin was “smooth” and “bulkyness” had disappeared, and 2 subjects had answered that “bulkyness” had disappeared. No subjects responded.

From the above results, the moisturizing fraction according to the present invention has a low concentration of 0.01% by weight, Also, it can be seen that the powder has an excellent moisturizing function.

Test Example 2: Skin effect test B (sensory test)

In order to evaluate the skin aging prevention effect of the cosmetic containing the moisturizing fraction obtained in Production Example 1, a skin beauty effect test (sensory test) was performed in the following manner.

The moisturizing fraction 5 O mg was kneaded into 500 g of white petrolatum to prepare an ointment treated with 0.01% by weight.

Next, the effect of applying this ointment to 10 middle-aged (aged 30 to 60) subjects washed with warm water until their fingers and hands became “bulky” was examined. The subject applied the appropriate amount of the ointment to the test site, and asked them to answer the condition before and after the test and the feeling of use. In order to eliminate the “stickiness” of Vaseline, hands were free to be washed and wiped off after application.

The test results were as follows: 9 subjects answered that their skin was “smooth” and “bulkyness” had disappeared, and 1 subject had answered that “bulkyness” had disappeared. No subjects responded.

From the above results, it can be seen that the cosmetic containing the moisturizing fraction according to the present invention gives moisture to the oil-deprived skin.

Test Example 3: Rough skin improvement effect test

A test for evaluating the rough skin improving effect of the cosmetic containing the moisturizing fraction obtained in Production Example 1 above was performed in the following manner.

An ointment prepared by mixing the moisturizing fraction of 5 O mg in 500 g of white petrolatum and treating with 0.01% by weight was prepared.

Next, the effect of application on the lower leg of 10 middle-aged and older (aged 30-65) subjects with rough skin was examined for 4 consecutive weeks. About 0.5 g of the ointment was applied to the left lower leg test site of the test subject once a day, and the state before and after the test was visually judged according to the following criteria. The right lower leg was used as a control without ointment (no treatment).

Judgment criteria for skin dryness

One: normal

Soil: slight drying, no desquamation

+: Dry, desquamation + +: Dry, moderate desquamation…

+++: Dry, remarkable desquamation

Compare the results of the test site before and after the test with the control site. If the dryness of the skin is improved by two or more levels (for example, 10-, + + →), it is considered effective. Valid, no change if invalid. The test results were 8 valid, 2 valid, and 0 invalid.

From the above results, it can be seen that the cosmetic containing the moisturizing fraction according to the present invention has the effect of improving rough skin and renewing the moisturizing function of the skin.

Test example 4: Cleaning resistance test

In order to evaluate the washing resistance of the moisturizing fraction according to the present invention, the following test was carried out using a vanishing cream containing the moisturizing fraction according to the present invention, which has the method of Preparation Example 10 described below. Was.

Distribute 2 g of burnishing cream to 10 subjects of middle-aged and elderly (age 30 to 62), and apply it freely to the back of hands and palms for 3 days. Then, the application of the burnishing cream was stopped. After the application of the burnishing cream, the number of hand washings when the “smooth” feel disappeared after washing the hands was asked.

The test results were as follows: 3 subjects who answered that the feeling of "smooth" did not disappear even after washing 5 times or more, 5 subjects who answered that the feeling of "smooth" did not disappear after 4 times, etc. None of the subjects answered one time and one subject answered twice, and none of the subjects answered that the feeling of “smoothness” disappeared by one washing.

From the above results, it can be seen that the cosmetic containing the moisturizing fraction according to the present invention does not lose its moisturizing film even after several washings, and retains its moisturizing properties.

Next, formulation examples are shown. The numerical value of the mixing ratio of each component in each formulation example represents "% by weight". Formulation Example 1: Lotion

1,3-butylene glycol 2.0

Ethanol 10.0

Methyl paraoxybenzoate 0.1

Moisturizing fraction from parotid gland secretion 0.0 1 Spice

Purified water residue

The lotion having the above-mentioned formula containing the moisturizing fraction according to the present invention moisturizes the skin and creates a "smooth" skin, and the following formulation examples make cosmetics extremely good on the skin. did.

Formulation Example 2: Emulsion

1,3-butylene glycol 3 0 squalane 2 0 stearic acid 2 0 liquid paraffin 6 0 sorbitan monostearate 1 5 polyoxyethylene (20) sorbitan monostearate 2 0 butyl parahydroxybenzoate 0 05 paraoxy Methyl benzoate 0 1 Moisturizing fraction from parotid gland secretion 0 01 Fragrance

Purified water residue

Formulation Example 3: Skin lotion

Ethanol 10.0

1, 3-butylene glycol 2.0 Glycerin 5.0 Methyl paraoxybenzoate 0.1 Moisturizing fraction derived from parotid gland secretion 0.02 Purified water residue

Formulation Example 4: Skin milk

Liquid paraffin 10 0 SENOLUNO 2 0 Cholesterin 0 5 Monoglyceride 1 0 Polyoxyethylene (6) sorbitan monohydrate 5.0 Moisturizing fraction derived from parotid gland secretion 0.02 Purified water Residue

Formulation Example 5: Massage milk

Olive oil 10.0 Octyldodecyl myristate 10.0 Squalane 10.0 Senoyl 2.0 Polyoxyethylene (5.5) cetyl ether 8.0 Polyoxyethylene (20) Sorbi quinone trioleate 5.0 ゥMoisturizing fraction derived from parotid gland secretion 0.02 Purified water residue

Formulation Example 6: Hair base conditioning water

1,3-Butylene glycol 2.0 Ethanol 10.0 Polyoxyethylene hydrogenated castor oil 10 0.05 Methyl parahydroxybenzoate 0.1 Moisturizing fraction from parotid gland secretion 0.02 Purified water residue

The above-mentioned hair base conditioning water containing the moisturizing fraction according to the present invention takes a few milliliters after shampooing, and when applied to hair and scalp, the hair becomes `` smooth '' and the hair styling material can be used thereafter. The combing was greatly improved. It also moisturized the scalp, enhanced the water retention function, and improved drying.

Formulation Example 7: Hair rinse

Seunor Knoll 1.0

1,3-butylene glycol 3.0 stearyltrimethylammonium chloride 4.5 stearic acid 0.5 monostearic acid 1.0 Polyoxyethylene (2) oleyl ether 3.5 Methyl paraoxybenzoate 0.1 Moisturizing fraction derived from parotid gland secretion 0.02 Purified water Residue Formulation example 8: Hand cream

White petrolatum 40.0 squalane 20.0 ceresin 5,0 sozorebi sesquioleate 3,0 polyoxyethylene sorbitan monooleate 0 8 propylene glycol 3 0 methyl parabenzoate 0 1 moisturizing oil derived from parotid gland secretion Min 0 01 fragrance

Purified water Residue Formulation Example 9: Cold cream

White ヮ serine 40.0 Cenosol 10.0 Salami beeswax 5.0.0 Sorbine sesquioleate 5 0 Lauromacrogol 0 5 Methyl paraoxybenzoate 0 1 Provir paraoxybenzoate 0 1 Moisturizing water from parotid gland secretion Sex fraction 0 01 Fragrance

Purified water Residue Formulation Example 10: Vanishing cream

White Pserin 25.0 Stearyl alcohol 20.0 Propylene glycol 2 · -0 Polyoxyethylene hydrogenated castor oil 64.0 Glycerin monostearate 10 0 Methyl parahydroxybenzoate 0 1 Provyl paraoxybenzoate 0 1 Moisturizing fraction from parotid gland secretion 0 0 1 Spice

Purified water Residue Formulation example 11 1: Skin cream

Liquid paraffin 1 5 5 Beeswax 3 3 0 Stearic acid 3 0 3.0 Sesinolate 3 0 3.0 Selenol 2.5 Polyoxyethylene (20) Sorbines monooleate 4 0 4 Glycerin 5.0 Paraoxybenzoic acid Methyl 0.1

N-lauroylu L-Glutamate sodium 0.2% Moisturizing fraction from parotid gland secretion 0.02 Purified water Residue Formulation example 12 2: Night cream

Liquid paraffin 15.0 Squalane 15, 0 Monoglyceride 2,0 Cholesterin 1,0 Ceanol 1 0 Cetyl palmitate 1 0 Lanolin 3 0 Polyoxyethylene (7) cetyl ether 8 0 Sorbitol 5.0 Methyl paraoxybenzoate 0.1 Moisturizing fraction from parotid gland secretion 0.0 2 Purified water Residual Industrial availability

The humectant, cosmetic and pharmaceutical product of the present invention contain a moisturizing fraction derived from a parotid gland secretion of a mammal as an active ingredient, and the moisturizing fraction has a very small amount of a moisturizing function. It can exert and enhance the moisture retention function of the skin.

The moisturizer, cosmetics and pharmaceuticals of the present invention can make the skin "moist" and improve the slip of the hair without causing discomfort such as "stickiness". Furthermore, the moisturizing function is highly durable, and even after several washings with water or hot water, the moisturizing film on the skin surface does not fall off.

In addition, by blending the moisturizing fraction into an external preparation for medicine, the function of retaining moisture on the skin surface is improved, and it works advantageously to bring out the medicinal properties of the medicine.

Claims

The scope of the claims -

1. A moisturizer characterized by containing, as an active ingredient, a fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid gland secretion of mammals.

2. The humectant according to claim 1, wherein the concentration of the water-miscible organic solvent is 63 to 80% (v / v).

3. The humectant according to claim 1, wherein the water-miscible organic solvent is acetone.

4. The humectant according to claim 1, wherein the mammal is a fish.

5. The fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid glands of mammals has a nitrogen content of 0.05 to 0.4% by weight and a protein content of 0.05% to 0.4% by weight. 2. The humectant according to claim 1, wherein the humectant is 0.4 to 3% by weight.

6. A method for moisturizing the skin, comprising applying the moisturizing agent according to claim 1 to the skin.

7. A method for moisturizing hair and scalp, which comprises applying the humectant according to claim 1 to the hair and / or scalp.

8. A cosmetic, comprising: a cosmetic base; and a fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or more in the parotid gland secretion of mammals.

9. The content of the fraction precipitated at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid gland secretion of mammals of 0.001 to 2% by weight. Cosmetics according to.

10. The cosmetic according to claim 8, wherein the concentration of the water-miscible organic solvent is 63 to 80% (v / v).

1 1. The cosmetic according to claim 8, wherein the water-miscible organic solvent is acetone.

12. The cosmetic according to claim 8, wherein the mammal is an insect.

13. The fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid gland secretion of mammals has a nitrogen content of 0.05 to 0.4% by weight and a protein content of 9. The cosmetic according to claim 8, which is in an amount of 0.4 to 3% by weight.

14. The cosmetic according to claim 8, which is a skin cosmetic or a hair cosmetic.

15. Skin cosmetics are lotions, emulsions, packs, creams, lotions, lipsticks, 15. The cosmetic according to claim 14, which is a foundation or a skin cleanser.

16. The cosmetic according to claim 14, wherein the hair cosmetic is a hair base conditioning water, a hair rinse, a hair tonic, a hair cream or a hair lotion.

17. It contains a fraction that precipitates at a concentration of 60% (v / v) of a water-miscible organic solvent in the parotid gland of mammals as a component of the base for external preparations for pharmaceuticals. Pharmaceuticals.

18. The content of the fraction precipitated at a water-miscible organic solvent concentration of 60% (v / v) or more in the parotid gland secretion of mammals of 0.001 to 2% by weight. Medical purple goods listed in.

19. The pharmaceutical according to claim 17, wherein the concentration of the water-miscible organic solvent is 63 to 80% (v / v).

20. The pharmaceutical product according to claim 17, wherein the water-miscible organic solvent is acetone.

21. The medicament according to claim 17, wherein the mammal is a mammal.

22. The fraction that precipitates at a water-miscible organic solvent concentration of 60% (v / v) or higher in the parotid gland secretion of mammals has a nitrogen content of 0.05 to 0.4% by weight and a protein content of 0.

18. The drug according to claim 17, which is 4-3% by weight.

23. The medicament according to claim 17, which is an external preparation selected from creams, ointments and lotions.