JP4424759B2 - Moisturizer, cosmetics and pharmaceuticals containing it - Google Patents

Description

Technical field
The present invention relates to a novel moisturizing agent, a moisturizing method using the same, and a cosmetic and a pharmaceutical containing the moisturizing agent. More specifically, the present invention is a novel moisturizing agent that is excellent in moisture retention and has a high moisture retention function, can give moist and smooth skin and hair, and is highly safe for the human body, The present invention relates to a method of moisturizing skin and hair using this moisturizing agent, and cosmetics and pharmaceuticals containing the moisturizing agent.
Background art
Skin cosmetics are generally prepared by blending a hydrophilic skin moisturizer and an oily emollient for the purpose of giving the skin appropriate moisture and oil to prevent the skin from drying out. .
As the hydrophilic skin moisturizer, polyethers such as polyethylene glycol and polypropylene glycol, glycerin, sorbitol and the like have been conventionally used, but in recent years, they are called natural moisturizing factors (NMF). Natural polymer compounds such as amino acids, sodium pyrrolidonecarboxylate, urea, sodium lactate, sodium hyaluronate, salivary gland mucin have come to be used.
However, conventionally used polyethers and humectants such as glycerin and sorbitol have the disadvantage of being dermatologically heterogeneous and absorb the moisture in the stratum corneum, which is the outermost layer of the skin. Rather, it may cause the skin to lose moisture, and NMFs that have been used in recent years are substances derived from natural products, but the feeling of use is poor and the moisturizing effect is poor. There are disadvantages such as.
On the other hand, sodium hyaluronate is currently used in many cosmetics and is useful because it gives the skin a “moist feeling” and “smoothness” in a concentration range of about 0.02 to 0.1% by weight. Has been highly evaluated. Moreover, in the safety test, local irritation and sensitization by experimental animals are not observed even in the concentration range of 2 to 3% by weight, and in the human patch test, it is safe even at a concentration of 1% by weight. It has been confirmed that there is.
Salivary gland mucin also has an excellent moisturizing action, and it is evaluated that it is superior to sodium hyaluronate in terms of “moistness” and “smoothness” given to the skin. Salivary gland mucin has a moisturizing action and an emulsifying action and has been confirmed to be highly safe for the human body, and can be said to be a rather ideal moisturizing agent. Salivary gland mucin is a glycoprotein that is biosynthesized and secreted in the salivary glands of mammals such as cows, horses, pigs, and sheep, that is, submandibular, parotid and sublingual glands. It has inherent viscosity depending on the kind of animal, and is obtained as a macromolecule having a molecular weight of about 100,000 to 1,000,000 containing a large amount of sialic acid.
Focusing on the excellent properties of such natural polymer compounds, in recent years as a moisturizer for cosmetics, pharmaceuticals, etc. (especially skin external preparations), and various industrial fields such as food, cosmetics, pharmaceuticals, quasi drugs, toiletries, etc. In Japan, research on utilization as an emulsifier has been promoted and a part of it has been put into practical use. However, the humectants made of these natural polymer compounds are disadvantageous in that the production cost is too high to be used in such an amount that the performance is sufficiently exhibited, and that the use of cosmetics for hair cannot be expected. There is.
Furthermore, cosmetics containing sodium hyaluronate and salivary gland mucin improve the feeling of use, but both components are easily decomposed, so that the molecular weight decreases with time, and the moisture retention and viscosity decrease. In addition, there is a drawback that the moisturizing film is easily removed by washing with water or hot water.
On the other hand, liquid paraffin, petrolatum, etc. are used as oily emollients, but if these emollients are added to an extent that inhibits normal moisture transpiration from the epidermis, they will give a sense of discomfort due to “stickiness”. Lack of usefulness as a skin moisturizer.
Disclosure of the invention
Under such circumstances, the first object of the present invention is as follows: (1) It exhibits moisture retention in a very small amount, and can be blended in various cosmetic bases and external pharmaceutical bases (2 ) Can give skin and hair “moist feeling” and “smoothness”, (3) Does not give a sense of incongruity such as “stickiness” and “skin” like conventional oily emollients, (4) Moisturizing It has excellent function sustainability, (5) the moisturizing film is not removed even after being washed several times with water or hot water, and (6) new safety features such as high safety to the human body. Providing a humectant.
A second object is to provide a method of moisturizing skin and hair using the moisturizing agent, and a third object is to provide cosmetics and pharmaceuticals containing the moisturizing agent. That is.
As a result of intensive studies to achieve the above-mentioned object, the present inventor obtained beautiful and smooth glossy hair when many animals rubbed their hair with their tongue and patched their saliva. In addition, attention is paid to the fact that the wound is also moistened with the tongue, moisturizes, prevents dryness, and accelerates the healing of the wound. Ingredients separated from the parotid secretion of mammals by a certain method (A) Moisturizing function in extremely small amount and safe for human body, (b) Even in powder form, it dissolves in water and exerts moisturizing function when applied to skin, (c) Various For example, it can be used by blending it into a cut cotton or paper as a powder, as an aqueous solution, or as a base for creams, lotions, or pharmaceutical external preparations. Increases moisture retention of skin even when used in addition to agents (D) It has excellent adsorptivity to the skin and gives the skin and hair “moist” and “smoothness”, while giving them a feeling of strangeness such as “stickiness” and “skin”. (E) Does not absorb moisture from the skin's stratum corneum more than necessary, and does not have the disadvantages of conventional hydrophilic moisturizers, (f) It has been found that it has excellent characteristics such as being resistant to washing with hot water, and the moisturizing film on the skin surface does not drop even by washing, and does not change the moisture retention state of the skin. It came to complete.
That is, the present invention
(1) A moisturizing agent comprising a fraction that precipitates at a concentration of 60% (v / v) or more of a water-miscible organic solvent in mammalian parotid secretion as an active ingredient,
(2) A method of moisturizing the skin, characterized by applying the moisturizer of (1) above to the skin,
(3) A method of moisturizing hair and scalp, characterized by applying the moisturizer of (1) above to the hair and / or scalp,
(4) A cosmetic comprising a cosmetic base and a fraction that precipitates at a concentration of 60% (v / v) or more of a water-miscible organic solvent in a parotid secretion of a mammal,
and
(5) As a component of a pharmaceutical external preparation base, it contains a fraction that precipitates at a concentration of 60% (v / v) or more of a water-miscible organic solvent in a parotid secretion of a mammal. Pharmaceuticals,
Is to provide.
BEST MODE FOR CARRYING OUT THE INVENTION
The moisturizing agent of the present invention contains a substance derived from the parotid secretion of a mammal as an active ingredient, and the mammal is not particularly limited. For example, cows, horses, pigs, sheep In view of the ease of collecting the secretion, the amount collected (about 50 to 60 liters per day is secreted in cows), the economy, and the like, cows are preferable.
Saliva secreted into the oral cavity of mammals (the secretion fluid flowing into the oral cavity) is a mixture of secretions from the parotid gland, submandibular gland and sublingual gland. The ratio of each salivary gland secretion to its secretion is approximately 70% by weight of parotid gland, 5% by weight of submandibular gland, and 25% by weight of sublingual gland. The mammalian secretion used in the present invention is limited to those secreted from the parotid gland, and is substantially free from the secretions secreted from the submandibular gland and sublingual gland, which are other salivary glands. Is. Saliva as a mixture contains mucous and serous components, but parotid secretions from the parotid glands are known to be serous and mostly water. ing. Until now, regarding “saliva”, only the whole saliva as a mixture has been studied, and there has been little research on the secretions of each of the above three glands. The present inventor has examined the secretions obtained from each salivary gland and found that the parotid secretion contains a component having an extremely excellent moisturizing function.
To collect mammalian parotid secretions, cut the skin under the animal's parotid, expose the parotid conduit near the oral epidermis, and slightly dispose the parotid conduit so that the tip of the cannula can be inserted. Make an incision. The cannula is guided from the skin incision, and the tip of the cannula is inserted from the incision of the parotid conduit toward the parotid gland. The wound at the skin incision is closed while the cannula is fixed with adhesive tape. A receptacle can be placed at the tip of the cannula and the parotid secretions flowing down naturally can be collected.
The collected parotid secretions should be stored in a cool place with a preservative added. Here, as the preservative to be added, conventional preservatives such as benzoic acids, paraoxybenzoic acids, dehydroacetic acids, sorbic acids, phenols, alcohols, halogens, glutaraldehydes, β-propiolactones, etc. Or mixtures thereof. Preferably, it is odorless or has an odor and can be easily removed in a later step, and paraoxybenzoic acids are particularly preferred.
The moisturizing agent of the present invention comprises a fraction that precipitates at a concentration of 60% (v / v) or more of an organic solvent by mixing a mammalian parotid secretion with a water-miscible organic solvent. In some cases) as an active ingredient. The reason for setting the organic solvent concentration to 60% (v / v) or more is as follows. As the organic solvent is added to the parotid secretion, the solubility of the moisturizing components in the parotid secretion decreases and precipitation begins to occur. The effective amount of organic solvent added is defined as the amount at which this precipitate can be substantially recovered, which is approximately 60% (v / v) or higher. Therefore, although this addition amount varies slightly depending on the type of organic solvent, practical precipitation cannot be expected if the addition amount of the organic solvent is less than 60% (v / v) in the present invention.
Thus, the concentration of the organic solvent is not particularly limited as long as it is 60% (v / v) or higher. However, considering the amount of precipitate to be formed and the economical efficiency due to the use of an excess organic solvent, the organic solvent is organic. The concentration of the solvent is preferably about 63 to 80% (v / v), more preferably about 65 to 75% (v / v).
The parotid gland secretion collected as described above is put as it is or after filtration into an extractor, and a water-miscible organic solvent is preferably added while stirring under cooling (about 0 to 15 ° C.) The fraction that precipitates at a solvent concentration of 60% (v / v) or higher is collected. The obtained moisturizing fraction may be used as it is, but it is advantageous to use it as a powder after dehydration and drying. As a dehydration method, there is a method in which a water-miscible organic solvent is further added to the obtained fraction. Moreover, as a drying method, there exist an aeration method, a pressure reduction method, etc., These methods can also be combined and it is also possible to add heating.
Examples of the water-miscible organic solvent used here include acetone, ethanol, methanol, and isopropanol. These may be used alone or in combination of two or more, but acetone is particularly preferable. Is preferred.
The yield of the moisturizing fraction depends on the health condition of the animal from which the parotid secretion is collected, but in general, 0.15 to the parotid secretion as a dry fraction. About 0.7% by weight. Further, the nitrogen content and the protein content in the dry moisturizing fraction depend on the health condition of the animal and the like, but generally 0.05 to 0.4% by weight and 0.4 to 0.4%, respectively. It is in the range of 3% by weight.
In addition, the substance having the moisturizing function of the moisturizing fraction has not yet been specified and is not limited to a single substance, but from the nitrogen content and protein content, mucopolysaccharides such as hyaluronic acid, salivary gland mucin, etc. Is considered to be a different substance.
In this way, the moisturizing fraction separated from the parotid secretion of the mammal has the following characteristics.
(1) It can give a “moist feeling” and “smoothness” to the skin and hair without causing unpleasant discomfort such as “tackiness” and “flickering”.
(2) The function of skin cells can be activated, the flexibility and elasticity of the skin can be increased, the skin can be kept healthy, and the skin can be given youth and luster.
(3) Even if it is in powder form or simply dissolved in water, it exhibits a moisturizing function for the skin, but when formulated in cosmetics and pharmaceutical external preparations, it exhibits an even better moisturizing function due to its synergistic effect. can do.
(4) Due to the high persistence of the moisturizing function, it is not necessary to reapply it over and over a day as in conventional cosmetics.
(5) Even by washing with water or hot water several times, the moisture retention film of the skin does not drop and the excellent moisture retention does not decrease.
(6) Even if it is used in a large amount, it is extremely safe without causing a risk of modulating physiological functions.
(7) Good miscibility with various components usually used in cosmetics and pharmaceutical external preparations, and there are no particular restrictions on the components to be mixed.
The moisturizing agent of the present invention contains the moisturizing fraction as an active ingredient, but the moisturizing agent may contain a conventionally known moisturizing component as desired. Known moisturizing components include, for example, polyethers such as polyethylene glycol and polypropylene glycol, polyhydric alcohols such as glycerin, 1,3-butylene glycol, propylene glycol and sorbitol, NMFs, specifically amino acids. And urea, sodium lactate, sodium pyrrolidone carboxylate, mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, and proteins such as collagen and elastin.
Next, the moisturizing method of the present invention will be described. The moisturizing method of the present invention has two modes, and one is a method of applying the moisturizing agent of the present invention to the skin and moisturizing the skin, whereby “smooth” and “smooth” are applied to the skin. Is given.
The other is a method of applying the moisturizing agent of the present invention to the hair and / or the scalp and moisturizing them, whereby the surface condition of the hair is improved and the surface of the hair is slipped (so-called comb). As the hair conditioner gets better, the scalp dryness is improved and itching and dandruff are reduced.
In these methods, the form of the moisturizing agent at the time of application is not particularly limited, and for example, a moisturizing agent can be used as various types of cosmetics described below and various types of pharmaceutical external preparations.
Next, the cosmetics and pharmaceuticals of the present invention will be described.
First, the cosmetic of the present invention contains a cosmetic base and a moisturizing fraction separated from the parotid secretion of the mammal.
The amount of the moisturizing fraction (dried state) contained in the cosmetic of the present invention depends on the form of the cosmetic and the presence or absence of other moisturizing ingredients, but is usually 0.001 based on the total amount of the cosmetic. ~ 2% by weight. If this content is less than 0.001% by weight, the moisturizing function may not be sufficiently exerted, and if it exceeds 2% by weight, no improvement in the effect is recognized for the amount, but it is economically disadvantageous. . Considering the moisturizing effect and economy, the preferred content is in the range of 0.005 to 0.2% by weight.
The base used in the cosmetic of the present invention is not particularly limited, and it is appropriately selected from various components conventionally used as the base in various forms of cosmetics according to the desired form of the cosmetic. Can do.
The form of the cosmetic of the present invention is not particularly limited. For example, skin cosmetics such as lotion, milky lotion, creams, packs, lotions, lipsticks, foundations, skin cleansers, hair basic adjustment water, hair rinses, hair arts Hair cosmetics such as hair cream and hair lotion can be mentioned, and the moisturizing fraction can be blended regardless of whether the base is aqueous or oily.
When the moisturizing fraction according to the present invention is blended with the skin cosmetic base, it can exhibit a further excellent moisturizing function due to a synergistic effect with the moisturizing function originally possessed by the cosmetic base. it can.
Further, a hair cosmetic comprising the hair cosmetic base formulated with the moisturizing fraction is excellent in the effect of keeping the hair and scalp healthy. For example, when applied to the hair and scalp after washing, the surface condition of the hair This improves the slip of the hair surface (so-called comb) and improves the riding of hair cosmetics. It can also improve scalp dryness and reduce itching and dandruff.
The cosmetics of the present invention include various components conventionally used in cosmetics, for example, oils, surfactants, emulsifiers, alcohols, other moisturizing ingredients, thickeners, antiseptics, antibacterials and fungi, as desired. Agents, antioxidants, chelating agents, pH adjusters, ultraviolet absorbers, ultraviolet scattering agents, pigments, dyes, fragrances, vitamins, amino acids, water and the like can be blended.
Examples of the oil include liquid oils such as castor oil, olive oil, jojoba oil, and cocoon oil, solid oils such as hardened castor oil, waxes such as lanolin, whale wax, beeswax, carnauba wax, and candelilla wax, squalane, petrolatum, Examples thereof include hydrocarbons such as liquid paraffin, sericin, and paraffin.
Examples of the surfactant include POE sorbitan esters such as polyoxyethylene (POE) sorbitan monooleate, sorbitan esters such as sorbitan monooleate, POE-glycerin fatty acid esters such as POE-glyceryl monooleate, and glycerin monooleate. POE-fatty acid esters such as POE-monooleate, POE-alkyl ethers such as POE-lauryl ether, POE-branched alkyl ethers such as POE-octyldodecyl ether, POE-alkyls such as POE-nonylphenyl ether Glycerol esters such as phenyl ether and glycerol monoisostearate, POE-glycerol esters such as POE-glycerol monoisostearate , Nonionic surfactants such as polyglycerin fatty acid esters such as diglyceryl monostearate, fatty acid soaps such as sodium and potassium salts of higher fatty acids such as stearic acid, higher alkyl sulfates such as sodium lauryl sulfate, POE-alkyl ether sulfates such as lauryl sulfate triethanolamine, N-acyl sarcosine salts such as sodium lauroyl sarcosine, higher fatty acid amide sulfonates such as sodium N-myristoyl-N-methyltaurate, sodium lineardodecylbenzene sulfonate Anionic surfactants such as sodium alkylbenzene sulfonate, N-acyl glutamate such as disodium N-stearoyl glutamate, alkylamine salts, POE-alkylamino Salts, polyamine fatty acid derivatives, alkylpyridinium salts, alkyl quaternary ammonium salts, alkyldimethylbenzylammonium salts, alkylisoquinolinium salts, dialkylmorpholinium salts, cationic surfactants such as benzethonium chloride, betaines, imidazolines And amphoteric surfactants such as amine oxides and amine oxides.
Examples of the alcohol include lower alcohols such as ethyl alcohol, propyl alcohol, and isopropyl alcohol, glycerin, polyglycerin, trimethylolpropane, pentaerythritol, dipentaerythritol, ethylene glycol, propylene glycol, polypropylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, further glucose, maltose, mannose, lactose, D-glucuronic acid, uronic acid, saccharose, D-mannitol, D-sorbit, sorbitan, gluconolactone, cellulose, starch, arbutin, glucose phosphorus Examples thereof include monosaccharides such as acid esters, polysaccharides, and derivatives thereof.
Examples of the other moisturizing components include those exemplified as the desired components in the description of the moisturizing agent of the present invention.
Examples of thickeners include natural polymeric substances such as sodium alginate, xanthan gum, marromero seed extract, tragacanth gum, starch, semi-synthetic polymeric substances such as methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, soluble starch, cationized cellulose, carboxy Synthetic polymer materials such as vinyl polymer and polyvinyl alcohol are exemplified.
Examples of the antiseptic / antifungal agent include methyl p-oxybenzoate, ethyl p-oxybenzoate, dehydroacetic acid, salicylic acid, benzoic acid, sorbic acid, benzalkonium chloride, and the like. Examples include dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, and ascorbic acid.
Examples of chelating agents include disodium edetate, ethanehydroxydiphosphate, pyrophosphate, hexametaphosphate, citric acid, tartaric acid, and gluconic acid. Examples of pH adjusters include sodium hydroxide, triethanolamine, and citric acid. Acid, sodium citrate, boric acid, borax, sodium monohydrogen phosphate and the like can be mentioned.
Furthermore, examples of the ultraviolet absorber include 2-hydroxy-4-methoxybenzophenone, octyldimethyl p-aminobenzoate, and ethylhexyl p-methoxycinnamate. Examples of the ultraviolet scattering agent include titanium oxide, kaolin, and talc. It is done.
Examples of vitamins include vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin F, vitamin K, vitamin P, vitamin U, carnitine, ferulic acid, α-oryzanol, α-lipoic acid, orot Acids and derivatives thereof include amino acids such as glycine, alanine, valine, leucine, isoleucine, serine, threonine, phenylalanine, tyrosine, tryptophan, cystine, cysteine, methionine, proline, hydroxyproline, aspartic acid, glutamic acid, arginine Histidine, lysine, and derivatives thereof.
On the other hand, the pharmaceutical of the present invention contains a moisturizing fraction separated from the mammalian parotid secretions as a component of a pharmaceutical external preparation base.
The pharmaceutical product of the present invention can improve the moisture retention function on the skin surface by applying it to the skin as an external preparation. Conventionally, in the case of urea compounds that are moisturizing ingredients added to pharmaceutical preparations for external use, there may be a `` feeling of skin '' feeling, pain, erythema, pruritus, burning sensation, desquamation, hypersensitivity, etc. May cause eczema, cracked skin, rarely swollen, dry.
The medicinal product of the present invention does not have the drawbacks of the above urea compounds, and is effective in improving cracks, redheads and other diseases caused by dry skin, rough skin, and dry skin. Furthermore, since the moisture retention function of the skin surface is improved, it has an advantageous effect to bring out the therapeutic effect of the pharmaceutical product.
The amount of the moisturizing fraction (dried state) according to the present invention contained in the pharmaceutical product of the present invention depends on the purpose of use, the dosage form, the presence or absence of addition of other moisturizing ingredients, etc. The range is 0.001 to 2% by weight based on the total amount. If the content is less than 0.001% by weight, the moisturizing function may not be sufficiently exhibited. If the content exceeds 2% by weight, the improvement in the effect is not recognized for the amount, but it is economically disadvantageous. Become. Considering the moisturizing effect and economy, the preferred content is in the range of 0.005 to 0.2% by weight.
The base used in the pharmaceutical product of the present invention is not particularly limited, and is appropriately selected from various components conventionally used as a base in various pharmaceutical external preparations according to the desired pharmaceutical dosage form. be able to.
The dosage form of the pharmaceutical product of the present invention is not particularly limited, and examples thereof include creams, ointments, lotions, and the like, whether the base is hydrophilic or lipophilic. The moisturizing fraction according to the present invention can be blended.
The drug that can be used in the pharmaceutical of the present invention is not particularly limited, and any drug that can be administered in the form of an external preparation can be used. In addition, the pharmaceutical may contain, as desired, for example, each component exemplified as the desired component in the description of the cosmetic, an anti-inflammatory agent, an antipruritic agent, a bactericidal disinfectant, a touch enhancer, a hormone agent, and the like. .
Hereinafter, the present invention will be described in more detail with reference to production examples, test examples, and formulation examples, but the present invention is not limited thereto.
Production Example 1
Bovine parotid secretion was collected, 0.15% by weight of methyl paraoxybenzoate was added as a preservative, and stored in a cool place. This parotid gland secretion was added to filter paper no. 2 (manufactured by Toyo Rossi Kaisha, Ltd.), 100 liters of the mixture was added in an extractor equipped with a cooling device at a temperature of 5 to 10 ° C. while adding 2 volumes (v / v) of acetone (acetone concentration 66 .6% (v / v)), a precipitate was formed and collected. An appropriate amount of acetone was added to the resulting precipitate for dehydration, and the acetone was filtered off to obtain an acetone dehydrated cake. This was pulverized, air-dried, and further dried at 60 ° C. under reduced pressure to obtain 436 g (yield 0.44%) of a moisturizing fraction derived from bovine parotid gland. The resulting moisturizing fraction had a nitrogen content of 0.13% by weight and a protein content of 1.06% by weight.
Test example 1: Skin effect test A (Sensory test)
In order to evaluate the skin aging prevention effect of the moisturizing fraction obtained in Production Example 1, a skin beautification test (sensory test) was performed as follows.
1100 ml of water is added to 20 mg of the moisturizing fraction and dissolved, and the entire amount is uniformly soaked in 200 g of Japanese Pharmacopoeia “Fabric Cotton” (manufactured by F3, Inc., cut cotton 5 × 5 cm) and heated. And dried to obtain a test sample. The content of the moisturizing fraction was 0.01% by weight with respect to the weight of the absorbent cotton.
Next, the application effect of this sample was examined on 10 middle-aged (aged 35-62) subjects who had been washed with warm water until their palms and the like became “bulky”. The subject's back and finger palm test sites were lightly rubbed with 0.5 g of the cut cotton a sufficient number of times, and the subsequent skin feel was answered. In addition, it was free to wash hands after application.
The test results were 8 subjects who responded that the skin was “smooth” and the “bulkyness” disappeared, and 2 subjects who answered that the “bulkyness” disappeared, and subjects who answered no change (invalid) I didn't.
From the above results, it can be seen that the moisturizing fraction according to the present invention has an excellent moisturizing function at a low concentration of 0.01% by weight and remains in powder form.
Test example 2: Skin beautiful effect test B (Sensory test)
In order to evaluate the skin aging prevention effect of the cosmetics containing the moisturizing fraction obtained in Production Example 1, a skin beautification effect test (sensory test) was performed as follows.
An ointment having a 0.01% by weight formulation was prepared by kneading 50 mg of the moisturizing fraction into 500 g of white petrolatum.
Next, the application effect of this ointment was examined on 10 middle-aged (aged 30 to 60) test subjects who were washed with warm water until their palms became “bulky”. An appropriate amount of the ointment was applied to the subject's finger palm test site, and the state before use and after the test and the feeling of use were answered. In order to eliminate the “stickiness” of petrolatum, it was free to wash and wipe hands after application.
The test results were 9 subjects who responded that the skin was “smooth” and the “bulkyness” disappeared, and 1 subject who answered that the “bulkyness” disappeared, and subjects who responded that there was no change (invalid) I didn't.
From the above results, it can be seen that the cosmetic containing the moisturizing fraction according to the present invention moisturizes the skin from which oil has been lost.
Test example 3: Skin roughening effect test
The test for evaluating the rough skin improvement effect of the cosmetic containing the moisturizing fraction obtained in Production Example 1 was performed as follows.
An ointment having a 0.01% by weight formulation was prepared by kneading 50 mg of the moisturizing fraction into 500 g of white petrolatum.
Next, the effect of continuous application for 4 weeks was examined on the lower legs of 10 middle-aged and older (aged 30 to 65) subjects with rough skin. About 0.5 g of the ointment was applied to the subject's left lower leg test site once a day, and the state before and after the start of the test was visually determined according to the following criteria. The right lower leg was used as a control with no ointment applied (no treatment).
Criteria for skin dryness
-: Normal
±: Lightly dry, no desquamation
+: Dry, desquamation light
++: Dryness, moderate desquamation
+++: Drying, noticeable desquamation
Compare the test results of the test site before and after the test with the control site, and when the skin dryness is improved by 2 or more levels (for example, + →-, ++ → ±), it is effective and when it is improved by 1 level, it is slightly effective The case where there was no change was invalidated. The test results were 8 effective, 2 effective, and 0 invalid.
From the above results, it can be seen that the cosmetic containing the moisturizing fraction according to the present invention has an effect of improving rough skin and renewing the moisturizing function of the skin.
Test example 4: Cleaning resistance test
In order to evaluate the washing resistance of the moisturizing fraction according to the present invention, the following test was performed using the vanishing cream containing the moisturizing fraction according to the present invention having the formulation of Preparation Example 10 described below.
Distribute 2g of vanishing cream to 10 middle-aged and older (aged 30-62) subjects and apply freely to the back of their hands and palms for 3 days (of course, they were free to wash their hands during that time), and then burnishing cream The application of was stopped. After the application of the vanishing cream was stopped, the number of washes was answered when the “smooth” feel disappeared after washing the hands.
As a result of the test, 3 subjects who answered that the “smooth” feeling did not disappear even after 5 or more washings, 5 subjects who answered that the “smooth” feeling disappeared after 4 times, and the following 3 times. None of the subjects answered that one subject answered one or two, and that the “smooth” feeling disappeared after one wash.
From the above results, it can be seen that the cosmetic containing the moisturizing fraction according to the present invention does not drop its moisturizing film and retains the moisturizing property even after several washes.
Next, formulation examples are shown. The numerical value of the blending ratio of each component in each formulation example represents “% by weight”.
Formulation Example 1: Lotion
1,3-butylene glycol 2.0
Ethanol 10.0
Methyl paraoxybenzoate 0.1
Moisturizing fraction derived from bovine parotid gland secretion 0.01
Perfume
Purified water residue
The lotion having the above-mentioned prescription containing the moisturizing fraction according to the present invention moisturizes the skin to create a “smooth” skin, and the following formulation example cosmetics get on the skin extremely well.
Formulation Example 2: Latex
1,3-butylene glycol 3.0
Squalane 2.0
Stearic acid 2.0
Liquid paraffin 6.0
Sorbitan monostearate 1.5
Polyoxyethylene (20) sorbitan monostearate 2.0
Butyl paraoxybenzoate 0.05
Methyl paraoxybenzoate 0.1
Moisturizing fraction derived from bovine parotid gland secretion 0.01
Perfume
Purified water residue
Formulation Example 3: Skin lotion
Ethanol 10.0
1,3-butylene glycol 2.0
Glycerin 5.0
Methyl paraoxybenzoate 0.1
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
Formulation Example 4: Skin milk
Liquid paraffin 10.0
Cetanol 2.0
Cholesterin 0.5
Monoglyceride 1.0
Polyoxyethylene (6) sorbitan monooleate 5.0
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
Formulation Example 5: Massage milk
Olive oil 10.0
Octyldodecyl myristate 10.0
Squalane 10.0
Cetanol 2.0
Polyoxyethylene (5.5) cetyl ether 8.0
Polyoxyethylene (20) sorbitan trioleate 5.0
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
Formulation Example 6: Hair foundation adjustment water
1,3-butylene glycol 2.0
Ethanol 10.0
Polyoxyethylene hydrogenated castor oil 10 0.05
Methyl paraoxybenzoate 0.1
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
The above-mentioned hair basal preparation water containing the moisturizing fraction according to the present invention takes several milliliters after shampooing, and when applied to the hair and scalp, the hair becomes “smooth”, and then the hair conditioner riding and combing are carried out. It was extremely improved. In addition, the scalp was moisturized, the water retention function was enhanced, and drying was improved.
Formulation Example 7: Hair rinse
Cetanol 1.0
1,3-butylene glycol 3.0
Stearyltrimethylammonium chloride 4.5
Stearic acid 0.5
Monostearic acid 1.0
Polyoxyethylene (2) oleyl ether 3.5
Methyl paraoxybenzoate 0.1
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
Formulation Example 8:hand cream
White petrolatum 40.0
Squalane 20.0
Ceresin 5.0
Sorbitan sesquioleate 3.0
Polyoxyethylene sorbitan monooleate 0.8
Propylene glycol 3.0
Methyl paraoxybenzoate 0.1
Moisturizing fraction derived from bovine parotid gland secretion 0.01
Perfume
Purified water residue
Formulation Example 9: Cold cream
White petrolatum 40.0
Cetanol 10.0
Sara honey bees 5.0
Sorbitan sesquioleate 5.0
Lauro Macrogol 0.5
Methyl paraoxybenzoate 0.1
Propyl paraoxybenzoate 0.1
Moisturizing fraction derived from bovine parotid gland secretion 0.01
Perfume
Purified water residue
Formulation Example 10: Vanishing cream
White petrolatum 25.0
Stearyl alcohol 20.0
Propylene glycol 12.0
Polyoxyethylene hydrogenated castor oil 60 4.0
Glycerol monostearate 1.0
Methyl paraoxybenzoate 0.1
Propyl paraoxybenzoate 0.1
Moisturizing fraction derived from bovine parotid gland secretion 0.01
Perfume
Purified water residue
Formulation Example 11: Skin cream
Liquid paraffin 15.0
Sara honey bee 3.0
Stearic acid 3.0
Sorbitan sesquioleate 3.0
Cetanol 2.5
Polyoxyethylene (20) sorbitan monooleate 4.0
Glycerin 5.0
Methyl paraoxybenzoate 0.1
Sodium N-lauroyl-L-glutamate 0.2
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
Formulation Example 12: Night cream
Liquid paraffin 15.0
Squalane 15.0
Monoglyceride 2.0
Cholesterin 1.0
Cetanol 2.0
Cetyl palmitate 1.0
Lanolin 3.0
Polyoxyethylene (7) cetyl ether 8.0
Sorbitol 5.0
Methyl paraoxybenzoate 0.1
Bovine parotid secretion derived moisturizing fraction 0.02
Purified water residue
Industrial applicability
The moisturizing agent, cosmetics and pharmaceuticals of the present invention contain a moisturizing fraction derived from mammalian parotid secretion as an active ingredient, and the moisturizing fraction has a very small amount of moisturizing function. Exerts and enhances the moisture retention function of the skin.
The moisturizing agent, cosmetics and pharmaceuticals of the present invention can “moist” the skin and improve the slipping of the hair without causing a sense of incongruity such as “stickiness”. In addition, the moisturizing function has a high durability, and the moisturizing film on the skin surface does not fall even after washing with water or hot water several times.
In addition, by blending the moisturizing fraction into a pharmaceutical external preparation, the moisture retention function of the skin surface is improved, and it has an advantageous effect to bring out the medicinal effect of the pharmaceutical.

Claims (12)

A moisturizing agent comprising, as an active ingredient, a fraction that precipitates by mixing acetone into a parotid secretion of a mammal so as to have a concentration of 63 to 80% (v / v). The humectant according to claim 1, wherein the mammal is a cow. A cosmetic comprising a cosmetic base and a fraction precipitated by mixing acetone in a parotid secretion of a mammal to a concentration of 63 to 80% (v / v) . To claim 3 content of fractions precipitated by admixing acetone parotid secretions of mammals to a concentration 63~80% (v / v) is 0.001 wt% Cosmetics described. The cosmetic according to claim 3 , wherein the mammal is a cow. The cosmetic according to claim 3 , which is a skin cosmetic or a hair cosmetic. The cosmetic according to claim 6 , wherein the skin cosmetic is a lotion, emulsion, pack, cream, lotion, lipstick, foundation or skin cleanser. The cosmetic according to claim 6 , wherein the hair cosmetic is hair basic conditioning water, hair rinse, hair nick, hair cream or hair lotion. As a component of a pharmaceutical external preparation base, it contains a fraction that precipitates by mixing acetone into a parotid secretion of a mammal to a concentration of 63 to 80% (v / v). Pharmaceutical products. To claim 9 content of fractions precipitated by admixing acetone parotid secretions of mammals to a concentration 63~80% (v / v) is 0.001 wt% The listed medicinal product. The pharmaceutical product according to claim 9 , wherein the mammal is a bovine. The pharmaceutical product according to claim 9 , which is a pharmaceutical external preparation selected from creams, ointments and lotions.