WO1998044099A3 - Methodes de detection d'interactions entre plusieurs proteines - Google Patents

Methodes de detection d'interactions entre plusieurs proteines Download PDF

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Publication number
WO1998044099A3
WO1998044099A3 PCT/FR1998/000685 FR9800685W WO9844099A3 WO 1998044099 A3 WO1998044099 A3 WO 1998044099A3 FR 9800685 W FR9800685 W FR 9800685W WO 9844099 A3 WO9844099 A3 WO 9844099A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
several proteins
detecting interaction
proteins
detecting
Prior art date
Application number
PCT/FR1998/000685
Other languages
English (en)
Other versions
WO1998044099A2 (fr
Inventor
Jacques Camonis
Francisco Romero-Portillo
Ricardo Attar
Jean-Marc Egly
Franck Tirode
Original Assignee
Inst Nat Sante Rech Med
Centre Nat Rech Scient
Jacques Camonis
Romero Portillo Francisco
Ricardo Attar
Egly Jean Marc
Franck Tirode
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Nat Sante Rech Med, Centre Nat Rech Scient, Jacques Camonis, Romero Portillo Francisco, Ricardo Attar, Egly Jean Marc, Franck Tirode filed Critical Inst Nat Sante Rech Med
Priority to JP54126398A priority Critical patent/JP2001522237A/ja
Priority to CA002285623A priority patent/CA2285623A1/fr
Priority to US09/402,317 priority patent/US6479237B1/en
Priority to EP98920567A priority patent/EP0977840A2/fr
Priority to AU73382/98A priority patent/AU7338298A/en
Publication of WO1998044099A2 publication Critical patent/WO1998044099A2/fr
Publication of WO1998044099A3 publication Critical patent/WO1998044099A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention a pour objet l'utilisation d'au moins un promoteur conditionnel pour la mise en oeuvre d'une méthode de détection d'une (ou plusieurs) protéine(s) interagissant directement ou indirectement avec au moins deux autres protéines dans le cadre de la formation d'un complexe protéique, ou de l'inhibition de la formation d'un complexe protéique.
PCT/FR1998/000685 1997-04-03 1998-04-03 Methodes de detection d'interactions entre plusieurs proteines WO1998044099A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP54126398A JP2001522237A (ja) 1997-04-03 1998-04-03 数種のタンパク質間の相互作用を検出する方法
CA002285623A CA2285623A1 (fr) 1997-04-03 1998-04-03 Methodes de detection d'interactions entre plusieurs proteines
US09/402,317 US6479237B1 (en) 1997-04-03 1998-04-03 Methods for detecting interaction among several proteins
EP98920567A EP0977840A2 (fr) 1997-04-03 1998-04-03 Methodes de detection d'interactions entre plusieurs proteines
AU73382/98A AU7338298A (en) 1997-04-03 1998-04-03 Methods for detecting interaction among several proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR97/04084 1997-04-03
FR9704084A FR2761697B1 (fr) 1997-04-03 1997-04-03 Methodes de detection d'interactions entre plusieurs proteines

Publications (2)

Publication Number Publication Date
WO1998044099A2 WO1998044099A2 (fr) 1998-10-08
WO1998044099A3 true WO1998044099A3 (fr) 1998-12-30

Family

ID=9505488

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1998/000685 WO1998044099A2 (fr) 1997-04-03 1998-04-03 Methodes de detection d'interactions entre plusieurs proteines

Country Status (7)

Country Link
US (1) US6479237B1 (fr)
EP (1) EP0977840A2 (fr)
JP (1) JP2001522237A (fr)
AU (1) AU7338298A (fr)
CA (1) CA2285623A1 (fr)
FR (1) FR2761697B1 (fr)
WO (1) WO1998044099A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7074902B1 (en) 1998-04-27 2006-07-11 Warf Wisconsin Alumni Research Foundation Antibody specific for a DNA repair protein
US7122343B1 (en) * 1998-04-27 2006-10-17 Wisconsin Alumni Research Foundation Methods to alter levels of a DNA repair protein
US20030104427A1 (en) * 2001-06-25 2003-06-05 Petrini John H.J. Methods to modulate telomere structure and function
CN116875518A (zh) 2018-07-06 2023-10-13 科罗拉多州立大学董事会法人团体 用于构建和检测生物活性剂的基因编码系统

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034646A1 (fr) * 1994-06-14 1995-12-21 American Cyanamid Company Nouveaux systemes cellulaires ayant une interaction specifique des paires de liaisons de peptides
WO1996030507A2 (fr) * 1995-03-31 1996-10-03 Amersham International Plc Vecteurs d'expression differentielle

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2253433A1 (fr) * 1996-04-29 1997-11-06 The Johns Hopkins University School Of Medicine Regulateur de la degradation de l'arn chez les mammiferes induite par des transcrits non-sens

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034646A1 (fr) * 1994-06-14 1995-12-21 American Cyanamid Company Nouveaux systemes cellulaires ayant une interaction specifique des paires de liaisons de peptides
WO1996030507A2 (fr) * 1995-03-31 1996-10-03 Amersham International Plc Vecteurs d'expression differentielle

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BUTT ET AL.: "COPPER METALLOTHIONEIN OF YEAST, STRUCTURE OF THE GENE, AND REGULATION OF EXPRESSION", PNAS, vol. 81, 1984, pages 3332 - 3336 *
FIELDS S ET AL: "A NOVEL GENETIC SYSTEM TO DETECT PROTEIN-PROTEIN INTERACTIONS", NATURE, vol. 340, 20 July 1989 (1989-07-20), pages 245/246, XP002018198 *
HINNEN A ET AL: "GENE EXPRESSION IN RECOMBINANT YEAST", 1 January 1995, GENE EXPRESSION IN RECOMBINANT MICROORGANISMS, PAGE(S) 121 - 193, XP000562527 *
JOHNSTON AND DAVIES: "SEQUENCES THAT REGULATE THE DIVERGENT GAL1-GAL10 PROMOTER IN SACCHAROMYCES CEREVISIAE", MOLECULAR AND CELLULAR BIOLOGY, vol. 4, 1984, pages 1440 - 1448, XP002051282 *
KERJAN ET AL.: "Nucleotide sequence of the Saccharomyces cerevisiae MET25 gene", NUCLEIC ACIDS RESEARCH, vol. 14, no. 20, 1986, pages 7861 - 7871, XP002051281 *
LEGRAIN AND CHAPON: "Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex", SCIENCE, vol. 262, 1993, pages 108 - 110, XP002051280 *
OSBORNE M A ET AL: "THE YEAST TRIBRID SYSTEM - GENETIC DETECTION OF TRANS-PHOSPHORYLATED ITAM-SH2-INTERACTIONS", BIO/TECHNOLOGY, vol. 13, December 1995 (1995-12-01), pages 1474 - 1478, XP002033515 *
TIRODE ET AL.: "A CONDITIONALLY EXPRESSED THIRD PARTNER STABILIZES OR PREVENTS THE FORMATION OF A TRANSCRIPTIONAL ACTIVATOR IN A THREE-HYBRID SYSTEM", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 37, 12 September 1997 (1997-09-12), pages 22995 - 22999, XP002051283 *
ZHANG J ET AL: "A YEAST THREE-HYBRID METHOD TO CLONE TERNARY PROTEIN COMPLEX COMPONENTS", ANALYTICAL BIOCHEMISTRY, vol. 242, November 1996 (1996-11-01), pages 68 - 72, XP002038711 *

Also Published As

Publication number Publication date
JP2001522237A (ja) 2001-11-13
AU7338298A (en) 1998-10-22
FR2761697B1 (fr) 1999-05-28
CA2285623A1 (fr) 1998-10-08
US6479237B1 (en) 2002-11-12
EP0977840A2 (fr) 2000-02-09
WO1998044099A2 (fr) 1998-10-08
US20020164587A1 (en) 2002-11-07
FR2761697A1 (fr) 1998-10-09

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