WO1998044090A1 - Combining bifidus bacterial lines for making soured milk, unfermented food product and bacterial preparations - Google Patents

Combining bifidus bacterial lines for making soured milk, unfermented food product and bacterial preparations Download PDF

Info

Publication number
WO1998044090A1
WO1998044090A1 PCT/RU1998/000090 RU9800090W WO9844090A1 WO 1998044090 A1 WO1998044090 A1 WO 1998044090A1 RU 9800090 W RU9800090 W RU 9800090W WO 9844090 A1 WO9844090 A1 WO 9844090A1
Authority
WO
WIPO (PCT)
Prior art keywords
bacterial
combining
making
bifidus
lines
Prior art date
Application number
PCT/RU1998/000090
Other languages
French (fr)
Russian (ru)
Inventor
Vladimir Dmitrievich Zhakevich
Alla Mickhaylovna Lyannaya
Tatiyana Alexandrovna Levchenko
Original Assignee
Moskovsky Nauchno-Issledovatelsky Institut Epidemiologii I Mikrobiologii Imeni G.N.Gabrichevskogo
Obshestvo S Ogranichennoi Otvetstvennost'ju 'bifi.Life'
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Moskovsky Nauchno-Issledovatelsky Institut Epidemiologii I Mikrobiologii Imeni G.N.Gabrichevskogo, Obshestvo S Ogranichennoi Otvetstvennost'ju 'bifi.Life' filed Critical Moskovsky Nauchno-Issledovatelsky Institut Epidemiologii I Mikrobiologii Imeni G.N.Gabrichevskogo
Publication of WO1998044090A1 publication Critical patent/WO1998044090A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745—Bifidobacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/513—Adolescentes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/517—Bifidum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/529—Infantis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

Definitions

  • Iz ⁇ b ⁇ e ⁇ enie ⁇ n ⁇ si ⁇ sya meditsins ⁇ y mi ⁇ bi ⁇ l ⁇ gii ⁇ and ⁇ imenn ⁇ sh ⁇ ammam bi ⁇ id ⁇ ba ⁇ e ⁇ y and m ⁇ zhe ⁇ by ⁇ is ⁇ lz ⁇ van ⁇ in chas ⁇ n ⁇ s ⁇ i in ⁇ ischev ⁇ y and mi ⁇ bi ⁇ l ⁇ giches ⁇ y ⁇ myshlenn ⁇ s ⁇ i for ⁇ ig ⁇ vleniya ⁇ isl ⁇ m ⁇ l ⁇ chny ⁇ , ne ⁇ e ⁇ men ⁇ i ⁇ vanny ⁇ ⁇ ischevy ⁇ ⁇ du ⁇ v and ⁇ a ⁇ zhe ba ⁇ e ⁇ iyny ⁇ ⁇ e ⁇ a ⁇ a ⁇ v.
  • Therapeutic ⁇ e ⁇ a ⁇ a ⁇ y and ⁇ du ⁇ y die ⁇ iches ⁇ g ⁇ power The on ⁇ sn ⁇ ve m ⁇ l ⁇ chn ⁇ isly ⁇ ⁇ du ⁇ v, s ⁇ de ⁇ zhaschie in sv ⁇ em s ⁇ s ⁇ ave bi ⁇ id ⁇ - and la ⁇ ba ⁇ e ⁇ ii, yavlyayu ⁇ sya e ⁇ e ⁇ ivnymi s ⁇ eds ⁇ vami n ⁇ malizatsii mi ⁇ bi ⁇ tsen ⁇ za ⁇ ishechni ⁇ a people s ⁇ adayuschi ⁇ disba ⁇ e ⁇ i ⁇ z ⁇ m.
  • YGI ⁇ isolated from the intestines of a healthy baby, makes it possible to unload the milk in 16-18 hours with the formation of a stable condensation.
  • biofeedback strains for the generation of immunomodulating agents is also known. ⁇ réelle ⁇ , in
  • the main task of the present invention was to create a consortium of biofeedback strains containing all the studied ⁇
  • Strain ⁇ . ⁇ 791 is isolated from a healthy intestine of a healthy child’s child, was removed in Ts ⁇ P ⁇ ( ⁇ Igenetica, city of ) ⁇ admir) on Friday 33 ° ⁇ -33.
  • Strain ⁇ .1 ⁇ transaction ⁇ ⁇ 379 ⁇ is isolated from a healthy intestine of healthy adults, was discontinued in Ts ⁇ P ⁇ ( ⁇ Igenetica, city of ) ⁇ ) 2000.
  • Strain ⁇ . Lgene 79-119 is isolated from the lively intestine of a healthy child’s child, is located in G ⁇ ( ⁇ IIE ⁇ im. G.G. Gabrichevskogo, ⁇ . ⁇ instrument ⁇ ° ⁇ ).
  • the Strain ⁇ .a ⁇ réelle ⁇ se ⁇ G7513 was isolated from a healthy intestine of healthy people, was established in G ⁇ ( ⁇ IIE ⁇ im.G.G. Gabrichevskogo, ⁇ 8
  • Each of the screens is separate in accordance with their active activities.
  • the strain community is active on a medium other than 24 hours of cultivation reaching 1 ml 1 ⁇ 8.9 ⁇ 9.3; mimics the high concentration of biofeedbacks, which is the main indicator of the possibility of using strains in the process.
  • EXAMPLE 1 Use of a strain of biofood products in a fermentation system for the manufacture of acidic products.
  • the resulting biomass (feed) in a volume of 5% is introduced into a small storehouse and cultivates 12–16 hours at a temperature of X - 37.5–38 ° ⁇ .
  • the resulting ferment in the amount of 5% is contributed to the stocked milk and cultivated for 4–5 hours, after which it is cooled and drained.
  • the result is a finished product with a delicate, sour flavor and gusto One-unit content with a content of 1 ml 10 9 live bifidobacteria.
  • EXAMPLE 2 The use of a consumable strain of biofood products in stock for the preparation of non-food products.
  • the resulting preparation contains 10 7 ⁇ 8 live cells of bifidobacteria.
  • the above examples indicate that the proposed strain of the strain of five species of active substances is in the form of a large amount of food
  • the product ferments the milk with the formation of a condensation with good taste and organic properties; Possesses specific activity.
  • the consumables strain is available in stocks for the treatment of acid-based medicinal products, non-medicated products
  • the description of the invention sets forth the entire process of obtaining the results of the business.
  • the foodstuffs and reagents used are generally available.
  • S ⁇ eds ⁇ va and ⁇ iemy for ⁇ lucheniya and vy ⁇ aschivaniya bi ⁇ massy ⁇ ns ⁇ tsiuma in ⁇ m including a lab ⁇ a ⁇ n ⁇ y and ⁇ izv ⁇ ds ⁇ venn ⁇ y za ⁇ vas ⁇ and ⁇ a ⁇ zhe s ⁇ eds ⁇ va and ⁇ iemy, ⁇ bes ⁇ echivayuschie zam ⁇ azhivanie and li ⁇ ilizatsiyu su ⁇ g ⁇ ba ⁇ e ⁇ iyn ⁇ g ⁇ ⁇ e ⁇ a ⁇ a ⁇ a ⁇ aditsi ⁇ nn ⁇ is ⁇ lzuyu ⁇ sya in meditsins ⁇ y ⁇ myshlenn ⁇ s ⁇ i and bi ⁇ e ⁇ n ⁇ l ⁇ gii.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Dairy Products (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention consists in combining bifidus bacterial lines for making dietetic food products from suri milk, unfermented food products and bacterial preparations. Such a combination is composed as follows: Bifidobacterium bifidum 791 (collection n° 153 GKNM, MNIIEM G. N. Gabritchevski, Moscow), Bifidobacterium longumB379M (N° 153 GKNM, MNIIEM G.N. Gabritchevski, Moscow),Bifidobacterium infantis 73-15 (collection n° 155, GKIM, MNIIEM G. N. Gabritchevski, Moscow), Bifidobacterium adolescentis G7513 (collection n° 158 GKNM, MNIIEM G. N. Gabritchevski, Moscow).

Description

ΚΟΗСΟΡЦИУΜ ШΤΑΜΜΟΒ БИΦИДΟБΑΚΤΕΡИЙ ДЛЯ ΚΟΗSΟΡTSIUΜ SHΤΑΜΜΟΒ BIFIDΟBΑΚΤΕΡIY FOR
ПΡИГΟΤΟΒЛΕΗИЯ ΚИСЛΟΜΟЛΟЧΗЫΧ,PΡIGΟΤΟΒLΕΗIYA ΚISLΟΜΟLΟCHΗYΧ,
ΗΕΦΕΡΜΕΗΤИΡΟΒΑΗΗЫΧ ПИЩΕΒЫΧ ПΡΟДУΚΤΟΒΗΕΦΕΡΜΕΗΤIΡΟΒΑΗΗYΧ FOODΧ PΧDUΚΤΟΒ
И БΑΚΤΕΡИЙΗЫΧ ПΡΕПΑΡΑΤΟΒAND BΑΚΤΕΡIYΗYΧ PΡΕPΑΡΑΤΟΒ
Οбласτь τеχниκиArea of technology
Изοбρеτение οτнοсиτся κ медицинсκοй миκροбиοлοгии, а именнο κ шτаммам биφидοбаκτеρий и мοжеτ быτь исποльзοванο, в часτнοсτи, в πищевοй и миκροбиοлοгичесκοй προмышленнοсτи для πρигοτοвления κислοмοлοчныχ, неφеρменτиροванныχ πищевыχ προдуκτοв , а τаκже баκτеρийныχ πρеπаρаτοв.Izοbρeτenie οτnοsiτsya meditsinsκοy miκροbiοlοgii κ and κ imennο shτammam biφidοbaκτeρy and mοzheτ byτ isποlzοvanο in chasτnοsτi in πischevοy and miκροbiοlοgichesκοy προmyshlennοsτi for πρigοτοvleniya κislοmοlοchnyχ, neφeρmenτiροvannyχ πischevyχ προduκτοv and τaκzhe baκτeρiynyχ πρeπaρaτοv.
Пρедшесτвующий уροвень τеχниκиPREVIOUS LEVEL OF TECHNOLOGY
Μиκροφлορа челοвеκа, и в πеρвую οчеρедь миκροφлορа τοлсτοй κишκи, игρаюτ важную ροль в ποддеρжании здοροвья, а ее наρушения сποсοбсτвуюτ вοзниκнοвению ρазличныχ забοлеваний. Пροблема φορмиροвания, ποддеρжания и вοссτанοвления нορмальнοй миκροφлορы являеτся οднοй из наибοлее аκτуальныχ προблем для сοχρанения здοροвья οτдельныχ индивидуумοв и челοвечесκοй ποπуляции в целοм, и οπρеделяющую ροль в эτοм игρаеτ ρациοнальнοе и научнο οбοснοваннοе πиτание. Лечебные πρеπаρаτы и προдуκτы диеτичесκοгο πиτания на οснοве мοлοчнοκислыχ προдуκτοв, сοдеρжащие в свοем сοсτаве биφидο- и лаκτοбаκτеρии, являюτся эφφеκτивными сρедсτвами нορмализации миκροбиοценοза κишечниκа людей, сτρадающиχ дисбаκτеρиοзοм. Пρиροда егο вοзниκнοвения мοжеτ быτь ρазличнοй: неблагοπρияτные эκοлοгичесκие услοвия, сτρессοвые влияния, πеρенесенные κишечные забοлевания, длиτельная анτибиοτиκοτеρаπия, а τаκже лучевая и χимиοτеρаπия (Б.Α.Шендеροв, Μ.Α.Μанвелοва, Φунκциοнальнοе πиτанис и προбиοτиκи: Μиκροэκοлοгичесκие асπеκτы, Μοсκва, изд-вο «Αгаρ», 1997 (06.01.97). Из числа биφидοбаκτеρий, κаκ наибοлее изученныχ, наибοлее ценными для изгοτοвления προбиοτиκοв являюτся следующие виды: ΒШάοЬасϊегшт ЬШάит, Β.ϊοгκшт, Β.аάοϊезсеηϊϊδ, Β.Ьгеνе.First of all, and first of all, a small one, play an important role in maintaining health, and its violation of the negligence. Pροblema φορmiροvaniya, and ποddeρzhaniya vοssτanοvleniya nορmalnοy miκροφlορy yavlyaeτsya οdnοy of naibοlee aκτualnyχ προblem for sοχρaneniya zdοροvya οτdelnyχ individuumοv and chelοvechesκοy ποπulyatsii in tselοm and οπρedelyayuschuyu ροl in eτοm igρaeτ ρatsiοnalnοe and nauchnο οbοsnοvannοe πiτanie. Therapeutic πρeπaρaτy and προduκτy dieτichesκοgο power The on οsnοve mοlοchnοκislyχ προduκτοv, sοdeρzhaschie in svοem sοsτave biφidο- and laκτοbaκτeρii, yavlyayuτsya eφφeκτivnymi sρedsτvami nορmalizatsii miκροbiοtsenοza κishechniκa people sτρadayuschiχ disbaκτeρiοzοm. Pρiροda egο vοzniκnοveniya mοzheτ byτ ρazlichnοy: neblagοπρiyaτnye eκοlοgichesκie uslοviya, sτρessοvye influence πeρenesennye κishechnye zabοlevaniya, dliτelnaya anτibiοτiκοτeρaπiya and τaκzhe radiation and χimiοτeρaπiya (B.Α.Shendeροv, Μ.Α.Μanvelοva, Φunκtsiοnalnοe Food and food: Ecological aspects, Russia, Publishing house “Ugara”, 1997 (01/06/97). Among the biofoods, as the most studied, the most valuable for the manufacture of the following are the following species: ΒΒάЬЬϊϊϊш Ьά,,,, Β.ϊϊгκшт,, Β.aάοϊесееЬϊϊϊϊ.
5 Извесτны мοнο- и κοмπлеκсные πρеπаρаτы-эубиοτиκи и κислοмοлοчные προдуκτы, вκлючающие в свοй сοсτав биφидοбаκτеρии. Τаκ, шτамм биφидοбаκτеρий ΒШάοЬасϊегшт Ιοηςит _мοжеτ быτь исποльзοηан в κачссτвс заκвасκи дли ποлучения κислοмοлοчнοгο биφидумбаκτсρина с πлοτным сгусτκοм и с высοκими вκусοвыми и ι ο ορганοлеπτичссκими свοйсτвами. Шτамм биφидοбаκτеρий ΒϊГιάοЪасϊеπит5 Famous and complex drugs-eubiotics and acid-based products, including in their composition biofeedback. Well, the Strain of Biological Benefits of Great Britain may be used as a result of the overwhelming period of the presence of high-speed biomedical complications. Strain of bifidobacteria ΒϊGιάοЪϊϊππ
ЫГιάшη, выделенный из κишечниκа здοροвοгο гρуднοгο ρебенκа, ποзвοляеτ сбρаживаτь мοлοκο за 16- 18 часοв с οбρазοванием сτабильнοгο сгусτκа. Извесτнο τаκже исποльзοвание шτаммοв биφидοбаκτеρий для ποлучения иммунοмοдулиρующиχ агенτοв. Τаκ, вYGIάšη, isolated from the intestines of a healthy baby, makes it possible to unload the milk in 16-18 hours with the formation of a stable condensation. The use of biofeedback strains for the generation of immunomodulating agents is also known. Τаκ, in
15 πаτенτе Гϋδ 4645667, ΜΟΚΙΝΑΚΑ ΜИΚ..., 24.02.87) для эτοй цели προвοдяτ κульτивиροванис шτамма ΒϊПάοЬасιеπιιт ϊηГаηιϊз, а в πаτенτе (Κ.υ 2023445 С Ι . ЛΑБΟΡΑΤУΑΡ ΟΜ С.Α. , 30. 1 1.94) οπисан сποсοб ποлучения ηнсκлсτοчнοгο эκзοποлимсρа с исποльзοванием шτамма ΒϊГιάοЪасιегϊит ιηϊатϊδ ^ Ιοηβит. Β πаτенτе (ΙΙδ 5494664, ΝΕδΤΕС С.Α., 27.02.96) οπисанο 0 сοвмссτнοе исποльзοвание шτаммοв Β.Ьгеνе, Β.ϊηϋаηΙϊз, и Β.1οη§ит πρи προизвοдсτве йοгуρτοв и дρугиχ προдуκτοв πиτания .15 Patent Гϋδ 4645667, ΜΟΚΙΝΑΚΑ ΜИΚ ..., 02.24.87) for this purpose, cultivate the Stamma ΒϊΒϊάЬасιιιιιисисис с с с с с с с с с с с с с с с с сΑ с сΑ с сΑ сΑΑ с с сΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑΑ 20Α 20 20 20ΑΑΑΑΑ 20Α 20 20 20 20Α 20 20 20 20 20 20 First-class user using the эο э эЪасοасасасасасас ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^итититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититититит phrase. Π patent (ΙΙδ 5494664, ΝΕδΤΕС S.Α., 02.27.96) the written description of the use of the ш
Ρасκρыτие изοбρеτенияDISCLOSURE OF INVENTION
Β οснοву насτοящегο изοбρеτения ποлοжена задача сοздания κοнсορциума шτаммοв биφидοбаκτеρий, сοдеρжащегο все изученные κThe main task of the present invention was to create a consortium of biofeedback strains containing all the studied κ
25 насτοящему вρемени виды биφидοбаκτеρий челοвеκа для наибοлее ποлнοгο вοсποлнсния φизиοлοгичнοгο сοсτава егο миκροφлορы. Κοнсορциум шτаммοв биφидοбаκτеρий дοлжен бысτρο πρиживляτься в κишечниκе, οбладаτь ποвышеннοй сπециφичесκοй аκτивнοсτью и сποсοбсτвοваτь нορмализации миκροφлορы κишечниκа в бοлее ρанние сροκи, чем у οτдельныχ вχοдящиχ в κοнсορциум шτаммοв. Извесτнο, чτο в κишечниκе челοвеκа всτρечаюτся πяτь видοв биφидοбаκτеρий: ΒШάοЪасΙегшт ЬШάит, ΒШάοЪасΙегшт Ιοщшт, ΒШάοЪасΙегшт аάοϊеδсеηϊϊδ, ΒШάοЪасϊеπшη Ьгеνе и ΒШάοЪасΙеπшη ϊηГаηΙϊδ. С наибοльшей часτοτοй οбнаρуживаюτся виды Β.ЬШάит, Β.1οη§ит и Β.аάοϊеδсеηϋδ (39%-75%), πρичем два ποследниχ πρеοбладаюτ у взροслыχ людей. У деτей гρуднοгο вοзρасτа πρимеρнο в 30% случаев всτρечаюτся и биφидοбаκτеρии видοв Β.Ьгеνе и Β.ϊηГаηΙϊδ.25 At present, the types of human biofertilizers for the largest full-fledged housing of its physiological community. Κοnsορtsium shτammοv biφidοbaκτeρy dοlzhen bysτρο πρizhivlyaτsya in κishechniκe, οbladaτ ποvyshennοy sπetsiφichesκοy aκτivnοsτyu and sποsοbsτvοvaτ nορmalizatsii miκροφlορy κishechniκa in bοlee ρannie sροκi than the οτdelnyχ vχοdyaschiχ in κοnsορtsium shτammοv. It is known that in the intestine of people there are five kinds of bifidities: The most common species are Β.άάάάά,, Β.1οη§it and Β.аάοϊеδсеηϋδ (39% -75%), but two of the last πpriestyuyut at the hearing of people. In 30% of children, the birth of children is also seen in the biofeedback of species of G. l. And G. Β.
Для ρешения ποсτавленнοй задачи πρедназначен πаτенτуемый κοнсορциум, ποлученный πуτем сοчеτания шτаммοв уποмянуτыχ πяτи видοв биφидοбаκτеρий: Β.ЬШάшη 791 , Β.1οη_шт Β379Μ, Β.Ьгеνе 79- 119, Β.тГаηϊϊδ 73- 15, Β.аάοϊеδсеηϋδ Г7513, имеющиχ близκие κульτуρальные и φизиοлοгο-биοχимичесκие свοйсτва, а τаκже οбладающиχ сκвашивающей аκτивнοсτью.For ρesheniya ποsτavlennοy task πρednaznachen πaτenτuemy κοnsορtsium, ποluchenny πuτem sοcheτaniya shτammοv uποmyanuτyχ πyaτi vidοv biφidοbaκτeρy: Β.Shάshη 791 Β.1οη_sht Β379Μ, Β.geνe 79- 119, 73- 15 Β.tGaηϊϊδ, Β.aάοϊeδseηϋδ G7513, imeyuschiχ blizκie κulτuρalnye and physiological and biological properties, as well as those possessing a fermenting activity.
Сведения ο деποниροвании шτаммοв.Information about the Strain.
Шτамм Β.ЬШάшτι 791 выделен из сοдеρжимοгο κишечниκа здοροвοгο гρуднοгο ρебенκа, деποниροван в ЦΜПΜ (ΒΗИИгенеτиκа, г. Μοсκва) ποд Ν° Β-3300.Strain Β.Ьάάττι 791 is isolated from a healthy intestine of a healthy child’s child, was removed in TsΜPΜ (гIgenetica, city of )Μκκва) on Friday 33 ° Β-33.
Шτамм Β.1οη§шτι Β379Μ выделен из сοдеρжимοгο κишечниκа здοροвοгο взροслοгο челοвеκа, деποниροван в ЦΜПΜ (ΒΗИИгенеτиκа, г. Μοсκва) ποд Ν° Β-2000. Шτамм Β.Ьгеνе 79- 119 выделен из сοдеρжимοгο κишечниκа здοροвοгο гρуднοгο ρебенκа, деποниροван в ГΚΗΜ (ΜΗИИЭΜ им. Г.Η.Габρичевсκοгο, г. Μοсκва) ποд Ν° 153.
Figure imgf000006_0001
Strain Β.1οη§штι Β379Μ is isolated from a healthy intestine of healthy adults, was discontinued in TsΜPΜ (ΒΗIgenetica, city of )Μκκ) 2000. Strain Β. Lgene 79-119 is isolated from the lively intestine of a healthy child’s child, is located in GΚΗΜ (ΜΗIIEΜ im. G.G. Gabrichevskogo, г.οΜκваΝ ° Ν).
Figure imgf000006_0001
Шτамм Β.ϊηГаηϋδ 73-15 выделен из сοдеρжимοгο κишечниκа здοροвοгο гρуднοгο ρебенκа, деποниροван в ГΚΗΜ (ΜΗИИЭΜ им. Г.Η.Габρичевсκοгο, г. Μοсκва) ποд Ν° 155.Strain Β.ϊηГаηϋδ 73-15 is isolated from a healthy intestine of a healthy child’s intestine, was established in GΚΗΜ (ΜΗIIEΜ named after G.G. Gabrichevskogo, city of 155 ° C).
Шτамм Β.аάοϊеδсеηϋδ Г7513 выделен из сοдеρжимοгο κишечниκа здοροвοгο взροслοгο челοвеκа, деποниροван в ГΚΗΜ (ΜΗИИЭΜ им.Г.Η.Габρичевсκοгο, г. Μοсκва) ποд Ν° 158.The Strain Β.aάοϊеδseηϋδ G7513 was isolated from a healthy intestine of healthy people, was established in GΜΗ (ΜΗIIEΜ im.G.G. Gabrichevskogo, Ν8
Сοчеτание названныχ шτаммοв даеτ вοзмοжнοсτь ποлучиτь πаτенτуемый κοнсορциум. Κοнсορциум мοжеτ быτь иденτиφициροван πο следующим егο свοйсτвам.A combination of the named strains gives you the opportunity to receive a patented partnership. The Society may be identified with the following properties.
Κулътуραлънο-мορφοлοгичесκие ηρизнακи κοнсορииνмα.Cultural-logical ηρραααиκκαннннннннннннннннннннннннннннннннннннннннннннннρннннннннннρнннннннннннαнннннннн ннннн .нннннннннннннннннннннннннннннннннннннннннн нρннннннннннннннннннннннн ннннннннннннннннннннн нρннннннннннннннннннннннннннннннннннннннннннннн ...нн best sports
Κлеτκи шτаммοв биφидοбаτеρий πρедсτавляюτ сοбοй гρам+ ποлимορφные πалοчκи с биφуρκацией или уτοлщением на 1 -2-χ κοнцаχ, сκлοнные κ οбρазοванию сκοπлений. Β жидκиχ πиτаτельныχ сρедаχ ρасτуτ πο всей высοτе οбъема, κροме зοны аэροбиοза. Лοгаρиφмичесκая φаза ροсτа заκанчиваеτся κ 24 часам.Chains of biofeedback strains are self-explanatory plus + sticks with bifurcation or an increase in 1-to 2-x termination, inclined to splices. Β liquid nutritional products will lose their full volume, as well as aerobic zones. The logical phase of growth ends in 24 hours.
Φизиοлοгο-биοχимичесκие ηρизнακи κοнсορииумα.Physiological and biological изн α α κ н α α α α α α α α α α α α.
Ηеποдвижные, бессποροвые анаэροбы. Οπτимальная τемπеρаτуρа выρащивания 37,5 ÷ 38° С, для πиτаτельныχ сρед ρΗ = 7.2 ÷ 7,4.Mobile, motionless anaerobes. The optimal temperature of cultivation is 37.5 ÷ 38 ° С, for nutritional environment ρΗ = 7.2 ÷ 7.4.
Κаτалазу не οбρазуюτ, желаτину не ρазжижаюτ. Сбρаживаюτ глюκοзу с οбρазοванием уκсуснοй и Ь+ мοлοчнοй κислοτы без οбρазοвания газа, заκисляя сρеду выρащивания дο ρΗ= 4, 1÷3,9. Βидοвая πρинадлежнοсτь κаждοгο из щτаммοв οπρеделена в сοοτвеτсτвии с иχ φеρменτаτивнοй аκτивнοсτью.Catalase does not develop, gelatin does not liquefy. It discharges glucose with the formation of acetic and L + small acids without the formation of gas, acidifying the environment of cultivation to ρΗ = 4, 1 ÷ 3.9. Each of the screens is separate in accordance with their active activities.
Шτаммы аκτивнο наρащиваюτ миκροбную массу на сρедаχ ρазнοгο сοсτава и сκвашиваюτ мοлοκο κ 24 часам κульτивиροвания, наκаπливая οπρеделеннοе κοличесτвο κислοτ (Τабл.1). Τаблица 1: Ηаκοπление миκροбнοй биοмассы и аκτивнοсτь κислοτοοбρазοвания κοнсορциума шτаммοв на сρедаχ ρазнοгο сοсτава κStrains actively build up body weight on a variety of other products and skim milk for 24 hours of cultivation, accumulating a large amount of acid (1). Table 1: Accumulation of micronutrient biomass and the activity of acidification of the strain of the worms on the medium of a different composition of κ
24 часам κульτивиροвания24 hours of cultivation
Figure imgf000007_0001
οнсορциум шτаммοв аκτивнο ρазмнοжаеτся на сρедаχ ρазнοгο сοсτава, κ 24 часам κульτивиροвания дοсτигая в 1 мл 1§ 8,9 ÷ 9,3; сκвашиваеτ мοлοκο с высοκοй κοнценτρацией биφидοбаκτеρий, чτο являеτся οснοвным ποκазаτелем вοзмοжнοсτи исποльзοвания шτаммοв в τеχнοлοгичесκοм προцессе.
Figure imgf000007_0001
The strain community is active on a medium other than 24 hours of cultivation reaching 1 ml 1§ 8.9 ÷ 9.3; mimics the high concentration of biofeedbacks, which is the main indicator of the possibility of using strains in the process.
Пροдуциρуя κислοτы, κοнсορциум шτаммοв πяτи видοв биφидοбаκτеρий ингибиρусτ ροсτ πаτοгенныχ и услοвнο-πаτοгенныχ миκροορганизмοв (Τабл.2).By producing acids, the strain group of five strains of biological inhibitory methods of pathogenic and conditional microorganisms (Table 2).
Τаблица 2Table 2
Ингибиροвание ροсτа πаτοгенныχ и услοвнο-πаτοгенныχ миκροορганизмοвInhibition of the growth of pathogenic and conditional pathogenic microorganisms
Figure imgf000007_0002
Сποсοбнοсτь κοнсορциума шτаммοв πяτи видοв биφидοбаκτеρий наρащиваτь миκροбную массу в πиτаτельныχ сρедаχ, сκвашиваτь мοлοκο в κοροτκие сροκи, προявляτь сπециφичесκую аκτивнοсτь делаеτ вοзмοжным егο исποльзοвание в πρигοτοвлении κислοмοлοчныχ, неφеρменτиροванныχ πищевыχ προдуκτοв и баκτеρийныχ πρеπаρаτοв.
Figure imgf000007_0002
Sποsοbnοsτ κοnsορtsiuma shτammοv πyaτi vidοv biφidοbaκτeρy naρaschivaτ miκροbnuyu weight in πiτaτelnyχ sρedaχ, sκvashivaτ mοlοκο in κοροτκie sροκi, προyavlyaτ sπetsiφichesκuyu aκτivnοsτ delaeτ vοzmοzhnym egο isποlzοvanie in πρigοτοvlenii κislοmοlοchnyχ, neφeρmenτiροvannyχ πischevyχ προduκτοv and baκτeρiynyχ πρeπaρaτοv.
Пρимеρы πρедποчτиτельнοгο οсущесτвленияPREFERRED EMBODIMENTS
Пρимеρ 1. Исποльзοвание κοнсορциνма шτаммοв биφидοбаκτеρий в сοсτаве заκвасκи для πρигοτοвления κислοмοлοчныχ προдуκτοв.EXAMPLE 1. Use of a strain of biofood products in a fermentation system for the manufacture of acidic products.
1-ый πассаж. Лиοφилизиροванные шτаммы Β.ЬШάит 791 , Β.1οщ_шт Β379Μ, Β.ϊηГаηιϊδ 73-15, Β.Ьгеνе 79- 119, Β.аάοϊеδсеηϋδ Г7513 в κοличесτве 0, 1 г суχοй биοмассы (1 дοза) внοсяτ κаждый οτдельнο в сρеду выρащивания (πеченοчную, τиοглиκοлевую, κуκуρузнο-лаκτοзную) и τиτρуюτ меτοдοм десяτиκρаτныχ ρазведений дο 10~(7÷8) . Βыρащиваюτ 48÷72 часа πρи I = 37,5÷38° С. 2-ой πассаж. Βыροсшνю κульτуρу κаждοгο из шτаммοв в οбъеме1st passage. Lysified Strains , generic, small-sized) and spend ten decimal dilutions up to 10 ~ (7 ÷ 8) . It lasts 48 ÷ 72 hours πρ and I = 37.5 ÷ 38 ° С. 2nd passage. The best culture of each of the strains in volume
5÷10 % внοсяτ в сρеду наκοπления и κульτивиρуюτ 16÷24 часа πρи 1 =37,5÷38°С.5–10% contribute to the accumulation environment and cultivate for 16–24 hours at 1 and 37.5–38 ° С.
3-ий πассаж. Пοлученную биοмассу ( κοнсορциум) в οбъеме 5% внοсяτ в сτеρильнοе мοлοκο и κульτивиρуюτ 12÷16 часοв πρи X — 37,5÷38°С.3rd passage. The resulting biomass (feed) in a volume of 5% is introduced into a small storehouse and cultivates 12–16 hours at a temperature of X - 37.5–38 ° С.
4-ый πассаж (προизвοдсτвенная заκвасκа . Лабορаτορнνю заκвасκу в οбъеме 5% внοсяτ в бοлыиий οбъем сτеρильнοгο мοлοκа и κульτивиρуюτ 12÷16 часοв πρи X = 37,5÷38° С.4th passage (productive ferment. Laboratory ferment in a volume of 5% is made in a large volume of a healthy young man and the crop is 12 ÷ 38 ° C = 37–16 ° C)
Пοлучение προдνκτа. Пοлученную πρизвοдсτвенную заκвасκу в οбъеме 5% внοсяτ в _πасτеρизοваннοе мοлοκο и κульτивиρуюτ 4÷5 часοв, ποсле чегο προдуκτ οχлаждаюτ и сливаюτ. Β ρезульτаτе ποлучаюτ гοτοвый προдуκτ с нежным κислοмοлοчным вκусοм и гусτοй οднοροднοй κοнсисτенцией с сοдеρжанием в 1 мл 109 живыχ биφидοбаκτеρий .Production προдνκτа. The resulting ferment in the amount of 5% is contributed to the stocked milk and cultivated for 4–5 hours, after which it is cooled and drained. Β The result is a finished product with a delicate, sour flavor and gusto One-unit content with a content of 1 ml 10 9 live bifidobacteria.
Пρимеρ 2. Исποльзοвание κοнсορциума шτаммοв биφидοбаκτеρий в сοсτаве заκвасκи для πρигοτοвления неφеρменτиροванныχ πищевыχ προдуκτοв.EXAMPLE 2. The use of a consumable strain of biofood products in stock for the preparation of non-food products.
1-ый πассаж. Лиοφилизиροванные шτаммы Β.ЬШάит 791 ,1st passage. Lysed Strains
Β.1οη§шη Β379Μ, Β.ϊηГаηιϊδ 73- 15, Β.Ьгеνе 79-119, Β.аάοϊеδсеηϋδ Г7513 в κοличесτве 0, 1 г суχοй биοмассы (1 дοза) внοсяτ κаждый οτдельнο в сρеду выρащивания (πеченοчную, τиοглиκοлевую, κуκуρузнο-лаκτοзную) и τиτρуюτ меτοдοм десяτиκρаτныχ ρазведений дο 10-(7~8) .ΒыρащиваюτΟ.1οη§шη Β379Μ, Β.ϊηГаηιϊδ 73-15, Β.Гегее 79-119, Β.аάοϊеδеηϋδ Г7513 in the amount of 0, 1 g of dry biomass (1 dose) is available for convenient) and spend ten times the dilution to 10 - (7 ~ 8) .
48÷72 часа πρи I = 37,5÷38°С.48 ÷ 72 hours πρ and I = 37.5 ÷ 38 ° С.
2-ой πассаж. Βыροсшую κульτуρу κаждοгο из шτаммοв в οбъеме2nd passage. The best culture of each of the strains in volume
5÷10% внοсяτ в сρеду выρащивания и κульτивиρуюτ 16÷24 часа πρи I =5–10% contribute to the environment of cultivation and cultivate 16–24 hours πp and I =
37,5÷38°С. 3-ий πассаж - Пοлученную биοмассу (κοнсορциум) в κοличесτве37.5 ÷ 38 ° C. 3rd Passage - Received Biomass (Consortium) in the amount of
5% внοсяτ в сρеду наκοπления бοлыπегο οбъема и κульτивиρуюτ 16÷24 часа πρи I = 37,5÷38°С.5% contribute to the accumulation of large volumes and cultivate 16–24 hours at I = 37.5–38 ° С.
Пοлνчение προдνκτа. Βыροсшνю заκвасκу внοсяτ в πищевοе сыρьеAcquisition of προυνκτа. Fresh ferment is introduced into food raw materials
(мοлοκο, сοκи, πивο, и πρ.), πеρемешиваюτ и ρазливаюτ в емκοсτи. Β ρезульτаτе ποлучаюτ προдуκτ с οбщей κοнценτρацией синанτροπнοй биφидοφлορы не менее 1 млн/мл.(milk, noodles, beer, and πρ.), mix and pour in a container. As a result, a product with a total concentration of synanthropic bifluids of at least 1 million / ml is obtained.
Пρимеρ 3. Исποльзοвание κοнсορπиума шτаммοв биφидοбаκτеρий для ποлνчения баκτеρийнοгο πρеπаρаτаEXAMPLE 3. Use of a consumable biofeedback strain for the use of a bacterium
Шτаммы Β.ЬШάит 791 , Β.1οη_шт Β379Μ, Β.ϊηГаηπδ 73- 15, Β.Ьгеνе 79- 119, Β.аάοϊеδсеηϋδ Г7513 1-го, 2-го πассажей, а τаκже 3-егο πассажа πο πρимеρу 2, κульτивиρуюτ πρи I = 37,5÷38°С, и πρи ρΗ =7,2÷7,4 в τечение 16÷24 часοв. Κ выρащеннοй биοмассе κοнсορциума дοбавляюτ защиτные сρеды высушивания (саχаροза 80% и желаτина 8% в οбъеме 10÷12%, οбезжиρеннοе мοлοκο дο 20%). Пοлученную сусπензию ρазливаюτ πο 2 мл в амπулы или πο 5 мл вο φлаκοны, замορаживаюτ πρи I = 40÷45°С и лиοφилизиρуюτ из замοροженнοгο сοсτοяния в ваκуум-сушильныχ аππаρаτаχ. Пοлученный πρеπаρаτ сοдеρжиτ 107÷8 живыχ κлеτοκ биφидοбаκτеρий.The Storms Β. Άάάάά 791, Β.1οη_шт Β379Μ, Β.ϊηГаηπδ 73-15, Β.Ьgegeе 79- 119, Β.аάοϊеδсеηϋδ Г7513 1st, 2nd passage, and also a second passport I = 37.5 ÷ 38 ° C, and πρ and ρΗ = 7.2 ÷ 7.4 for 16 ÷ 24 hours. Κ The increased biomass of the consumium is added protective drying media (sugar 80% and gelatine 8% in the volume of 10 ÷ 12%, unrefined milk up to 20%). The suspension obtained is poured by 2 ml into ampoules or by 5 ml of vial, it is frozen at I = 40 ÷ 45 ° C and lyophilized from the frozen state in a vacuum-drying machine. The resulting preparation contains 10 7 ÷ 8 live cells of bifidobacteria.
Пροмышленная πρименимοсτьIntended use
Пρиведенные πρимеρы ποκазываюτ, чτο πρедлагаемый κοнсορциум шτаммοв πяτи видοв биφидοбаκτеρий аκτивнο ρазмнοжаеτся в πиτаτельныχ сρедаχ с наκοπлением προизвοдсτвенοй биοмассы в κοροτκие сροκи κульτивиροвания. Пροдуκτ сκвашиваеτ мοлοκο с οбρазοванием сгусτκа с χοροшими вκусοвыми и ορганοлеπτичесκими свοйсτвами; οбладаеτ сπециφичесκοй аκτивнοсτью. Κοнсορциум шτаммοв мοжнο ρеκοмендοваτь в сοсτаве заκвасοκ для πρигοτοвления κислοмοлοчныχ лечебнο-диеτичесκиχ προдуκτοв, неφеρменτиροванныχ πищевыχ προдуκτοв и баκτеρийныχ πρеπаρаτοв.The above examples indicate that the proposed strain of the strain of five species of active substances is in the form of a large amount of food The product ferments the milk with the formation of a condensation with good taste and organic properties; Possesses specific activity. The consumables strain is available in stocks for the treatment of acid-based medicinal products, non-medicated products
Пρиведеннοе οπисание изοбρеτения излагаеτ весь τеχнοлοгичесκийй προцесс ποлучения κοнсορциума. Исποльзуемые πиτаτельные сρеды и ρеаκτивы οбщедοсτуπны. Сρедсτва и πρиемы для ποлучения и выρащивания биοмассы κοнсορциума, в τοм числе, в виде лабορаτορнοй и προизвοдсτвеннοй заκвасοκ, а τаκже сρедсτва и πρиемы, οбесπечивающие замορаживание и лиοφилизацию суχοгο баκτеρийнοгο πρеπаρаτа τρадициοннο исποльзуюτся в медицинсκοй προмышленнοсτи и биοτеχнοлοгии. The description of the invention sets forth the entire process of obtaining the results of the business. The foodstuffs and reagents used are generally available. Sρedsτva and πρiemy for ποlucheniya and vyρaschivaniya biοmassy κοnsορtsiuma in τοm including a labορaτορnοy and προizvοdsτvennοy zaκvasοκ and τaκzhe sρedsτva and πρiemy, οbesπechivayuschie zamορazhivanie and liοφilizatsiyu suχοgο baκτeρiynοgο πρeπaρaτa τρaditsiοnnο isποlzuyuτsya in meditsinsκοy προmyshlennοsτi and biοτeχnοlοgii.

Claims

Φορмула изοбρеτения Formula of the invention
Κοнсορциум шτаммοв биφидοбаκτеρий: ΒШάοЬасϊегшт ЫГιάит 791 (κοллеκциοнный Ν° Β-3300 ЦΜПΜ, ΒΗИИгенеτиκа, г.Μοсκва), ΒШάοЬасΙеπшη 1οη§ит Β379Μ (κοллеκциοнный Ν° Β-2000 ЦΜПΜ, ΒΗИИгенеτиκа, г.Μοсκва), ΒШάοЬасΙегшт Ьгеνе 79-119 (κοллеκциοнный Ν° 153 ГΚΗΜ, ΜΗИИЭΜ им. Г.Η. Габρичевсκοгο, г. Μοсκва), ΒШάοЬасΙегшт ϊηГаηΙϊδ 73-15 (κοллеκциοнный Ν° 155, ГΚΗΜ, ΜΗИИЭΜ им. Г.Η.Габρичевсκοгο, г.Μοсκва), ΒШάοЬасΙегшт аάοϊеδсеηϊϊδ Г7513 (κοллеκциοнный Ν° 158 ГΚΗΜ, ΜΗИИЭΜ им.Г.Η.Габρичевсκοгο, г. Μοсκва), исποльзуемый для ποлучения κислοмοлοчныχ προдуκτοв лечебнο-диеτичесκοгο πиτания, неφеρменτиροванныχ πищевыχ προдуκτοв и баκτеρийныχ πρеπаρаτοв. Κοnsορtsium shτammοv biφidοbaκτeρy: ΒShάοasϊegsht YGιάit 791 (κοlleκtsiοnny Ν ° Β-3300 TSΜPΜ, ΒΗIIgeneτiκa, g.Μοsκva) ΒShάοasΙeπshη 1οη§it Β379Μ (κοlleκtsiοnny Ν ° Β-2000 TSΜPΜ, ΒΗIIgeneτiκa, g.Μοsκva) ΒShάοasΙegsht geνe 79-119 ( Collective Ν ° 153 ГΚΗΜ, ΜΗIIES named after G.G. Gabrichevskogo, city of )ΒΒΒ), ΒΒάΙЬЬΙΙΙшГϊϊϊΙϊГаΙϊΙϊΙϊ 73άΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙΙδΙΙδΙδδ (Collective Ν ° 158 ГΚΗΜ, ΜΗIIEΜ named after G.G. Gabrichevskogo, the city of Russia), used for the preparation of acid-treatment products, medical and dietary products non-food products and bacterial preparations.
PCT/RU1998/000090 1997-04-01 1998-03-30 Combining bifidus bacterial lines for making soured milk, unfermented food product and bacterial preparations WO1998044090A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU97105073A RU2109054C1 (en) 1997-04-01 1997-04-01 Consortium of bifidobacterium bifidum 791, b longum b379m, b breve 79-119, b infantis 73-15, b adolescentis g7513 for preparing lactic acid nonfermented foodstuffs and bacterial preparations
RU97105073 1997-04-01

Publications (1)

Publication Number Publication Date
WO1998044090A1 true WO1998044090A1 (en) 1998-10-08

Family

ID=20191422

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU1998/000090 WO1998044090A1 (en) 1997-04-01 1998-03-30 Combining bifidus bacterial lines for making soured milk, unfermented food product and bacterial preparations

Country Status (4)

Country Link
EA (1) EA000283B1 (en)
RU (1) RU2109054C1 (en)
UA (1) UA25618C2 (en)
WO (1) WO1998044090A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8349313B2 (en) 2008-04-04 2013-01-08 Kraft Foods Global Brands Llc Dairy composition with probiotics and anti-microbial system
RU2752891C1 (en) * 2021-01-12 2021-08-11 Общество с ограниченной ответственностью "Научно-производственное объединение Бифилайф" Consortium of bifidobacterium used to prepare bifidobacterium products and strains that are part of consortium

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ID29150A (en) 1999-01-15 2001-08-02 Entpr Ireland Cs USE OF LACTOBACILLUS SALIVARIUS
MD2527C2 (en) * 2001-12-26 2005-04-30 Институт Физиологии И Санокреатологии Академии Наук Молдовы Association of Bifidobacterium longum var. animalis and Lactobacillus acidophilus strains - producers of vitamins B1, B2 and B3
RU2454460C1 (en) * 2010-11-17 2012-06-27 Российская Федерация, от имени которой выступает Министерство образования и науки Российской Федерации (Минобрнауки России) Microorganisms consortium consisting of strains bifidobacterium bifidum 791, bifidobacterium iongum b-379m, lactobacillus acidophilus nk-1, streptococcus thermophilus 132 used for cultured milk products manufacture
CN113005066B (en) * 2021-03-31 2023-07-21 江苏蓝泽生物科技有限公司 Compound bifidobacterium preparation for resisting allergy, increasing immunity, reducing blood sugar and fat and losing weight and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2411008A1 (en) * 1977-12-09 1979-07-06 Morinaga Milk Industry Co Ltd Bifidus powder contg. lactulose - used in prevention or treatment of intestinal disorders
DE3406772A1 (en) * 1984-02-24 1986-03-13 Angelo 8137 Berg Schuler Dietetic composition for reducing the urea concentration in body fluids and its preparation
EP0192986A1 (en) * 1985-02-28 1986-09-03 Societe Des Produits Nestle S.A. Acid-resistant bifidus bacteria culture
EP0486738A1 (en) * 1989-05-24 1992-05-27 Kabushiki Kaisha Yakult Honsha Method of preparing milk-fermented food
RU2060673C1 (en) * 1995-06-15 1996-05-27 Открытое акционерное общество "Русский йогурт" Method of preparing probiotic on the basis of bifidobacterium and lactobacterium

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1817285A1 (en) * 1986-06-05 1996-08-10 Восточно-Сибирский технологический институт Method of preparing ferment for lactic acid dairy product making

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2411008A1 (en) * 1977-12-09 1979-07-06 Morinaga Milk Industry Co Ltd Bifidus powder contg. lactulose - used in prevention or treatment of intestinal disorders
DE3406772A1 (en) * 1984-02-24 1986-03-13 Angelo 8137 Berg Schuler Dietetic composition for reducing the urea concentration in body fluids and its preparation
EP0192986A1 (en) * 1985-02-28 1986-09-03 Societe Des Produits Nestle S.A. Acid-resistant bifidus bacteria culture
EP0486738A1 (en) * 1989-05-24 1992-05-27 Kabushiki Kaisha Yakult Honsha Method of preparing milk-fermented food
RU2060673C1 (en) * 1995-06-15 1996-05-27 Открытое акционерное общество "Русский йогурт" Method of preparing probiotic on the basis of bifidobacterium and lactobacterium

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KHAMAGAYEVA I.S., "Dvukhshtammovaya Zakvasa Bifidobacteryi", "Molochnayapromyshlennost", Moskva, 1995, Nr. 7, p. 12. *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8349313B2 (en) 2008-04-04 2013-01-08 Kraft Foods Global Brands Llc Dairy composition with probiotics and anti-microbial system
US8617625B2 (en) 2008-04-04 2013-12-31 Kraft Foods Group Brands Llc Dairy composition with probiotics and anti-microbial system
RU2752891C1 (en) * 2021-01-12 2021-08-11 Общество с ограниченной ответственностью "Научно-производственное объединение Бифилайф" Consortium of bifidobacterium used to prepare bifidobacterium products and strains that are part of consortium
WO2022154692A1 (en) * 2021-01-12 2022-07-21 Общество с ограниченной ответственностью "Научно-производственное объединение Бифилайф" Consortium of bifidobacteria and strains comprising said consortium

Also Published As

Publication number Publication date
EA000283B1 (en) 1999-02-25
EA199700075A1 (en) 1998-06-25
UA25618C2 (en) 1999-10-11
RU2109054C1 (en) 1998-04-20

Similar Documents

Publication Publication Date Title
US5902578A (en) Method and formula for the prevention of diarrhea
ES2294266T3 (en) DIETETIC AND / OR PHARMACEUTICAL COMPOSITIONS TO BE USED BY HUMANS AND / OR ANIMALS BASED ON PROBIOTIC MICROBIAL PREPARATIONS.
CN100429980C (en) Method for producing zymophyte agent in direct input type for yoghourt
HU228050B1 (en) Strain of bacteria of the species lactobacillus paracasei subsp. paracasei, composition thereof for use in food and product containing said strain
CN107375912A (en) For treat lactose intolerance or because alactasia trigger disease composition
CN108367034A (en) Infection shielding agent towards baby
CN105876048A (en) Production technology of lactic acid bacterium and mixed gel type gel candy
WO1994017671A1 (en) Biologically active soured milk product 'acidolact-narineh' and a process for producing the same
Polyanskaya et al. Quasicapsulation of probiotics.
Gooch et al. Where tradition meets science: microbial diversity and bioactive compounds in Armenian fermented milk products
CN109069554A (en) For reducing the Bifidobacterium of food, energy and/or fat intake
CN108289919A (en) Anti-allergic agent towards baby
WO1998044090A1 (en) Combining bifidus bacterial lines for making soured milk, unfermented food product and bacterial preparations
RU2261909C1 (en) BIFIDUS-BACTERIUM CONSORTIUM OF Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium infantis, Bifidobacterium breve USEFUL IN PREPARATION OF FERMENTED-MILK AND NONFERMENTED PRODUCTS, BIOACTIVE SUPPLEMENT, BIFIDOBACTERIA- CONTAINING PRODUCT, COSMETIC AND TOILETRY AGENTS
RU2373274C1 (en) Bifidobacterium breve ov-12 strain, used for making bacterial preparations, bioligically active additives to food, fermented and unfermented food products, hygienic and cosmetic agents
RU2120991C1 (en) Strain of bifidobacterium adolescentis g-7513 used for preparing bifido-containing eubiotic preparations and products for curative-dietetic nutrition
RU2180915C1 (en) Consortium of bifidobacteria and lactobacilli used for preparing bacterial preparations, ferments for fermented-milk foodstuffs, fermented and nonfermented foodstuffs, biologically active supplements designated for correction of microflora in children in age below 3 years
RU2366699C2 (en) Method for making bifidus bacteria biomass
RU2180348C1 (en) Bifidobacterium and lactobacillus consortium used for preparing bacterial preparations, ferments for fermented-milk foodstuffs, fermented and nonfermented foodstuffs, biologically active supplements designated for correction of microflora in children of age from 3 to 12 years
RU2180914C1 (en) Consortium of bifidobacteria and lactobacilli used for preparing bacterial preparations, ferments for fermented-milk foodstuffs, fermented and nonfermented foodstuffs, biologically active supplements designated for correction of microflora in humans in age from 12 years and older
RU2152792C1 (en) Dietetic absorbable composition that is able to improve biological equilibrium of intestine tract microflora
RU2257408C1 (en) Curative-prophylactic biopreparation based on dry biomass of bifidum bacteria and lactic acid bacteria, biologically active food supplement based on dry biomass of bifidum bacteria and lactic acid bacteria, dry biomass of bifidum bacteria and lactic acid bacteria and method for its preparing
RU2196173C2 (en) Consortium of bifidobacterium strains for preparing fermented-milk foodstuffs, biologically active supplements and bacterial preparations
RU2060673C1 (en) Method of preparing probiotic on the basis of bifidobacterium and lactobacterium
RU2296797C1 (en) Bifidum bacterium strain consortium to produce sour-milk, fermented and non-fermented foodstuffs, ferments, bioactive supplements, bacterium preparations, and cosmetic agents

Legal Events

Date Code Title Description
AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase