WO1998025626A1 - Utilisation de l'equilenine comme antioxydant - Google Patents

Utilisation de l'equilenine comme antioxydant Download PDF

Info

Publication number
WO1998025626A1
WO1998025626A1 PCT/US1997/022154 US9722154W WO9825626A1 WO 1998025626 A1 WO1998025626 A1 WO 1998025626A1 US 9722154 W US9722154 W US 9722154W WO 9825626 A1 WO9825626 A1 WO 9825626A1
Authority
WO
WIPO (PCT)
Prior art keywords
equilenin
free radical
pharmaceutically acceptable
sulfate ester
disease
Prior art date
Application number
PCT/US1997/022154
Other languages
English (en)
Inventor
Steven Jay Adelman
Dorothy Helen Prozialeck
Original Assignee
American Home Products Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Home Products Corporation filed Critical American Home Products Corporation
Priority to AU53707/98A priority Critical patent/AU743586B2/en
Priority to CA002272087A priority patent/CA2272087A1/fr
Priority to BR9714384-7A priority patent/BR9714384A/pt
Priority to NZ336343A priority patent/NZ336343A/xx
Priority to EP97950808A priority patent/EP0944391A1/fr
Priority to IL13007197A priority patent/IL130071A0/xx
Priority to JP52680498A priority patent/JP2001506628A/ja
Priority to HU0000569A priority patent/HUP0000569A3/hu
Publication of WO1998025626A1 publication Critical patent/WO1998025626A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/565—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/565—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
    • A61K31/566—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol having an oxo group in position 17, e.g. estrone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants

Definitions

  • Biologically generated free radicals have been implicated in a large number of disease states.
  • the survival of aerobic organisms in an oxygen environment involves a complicated interplay between the biological generation of these very reactive chemical species and the ability of the organism to control them (Del Maestro RF, Acta Phy Scan Suppl. 492:153-68 (1980)).
  • This interplay between the host organism and biologically generated free radicals results in profound biochemical alterations which culminate in cellular injury and death of the organism.
  • the accumulated products of free radical reactions result in some of the large number of disease conditions which have been suggested to result, in part, from cellular injury induced by an increased flux of intracellular free radicals.
  • These include, but are not limited to cancers, cardiovascular disease, central nervous system disorders, bone disease, aging, Alzheimer's dementia, inflammatory disorders, rheumatoid arthritis, autoimmune diseases, respiratory distress and emphysema.
  • Oxidation and the use of antioxidants is also important for the treatment of numerous inflammatory disease states.
  • Rheumatoid arthritis is the most common chronic inflammatory disease.
  • Epidemiological studies reveal a prevalence rate of classical and definite RA between 0.3 and 1.5 percent.
  • Joint disease with chronic persistent inflammation is accompanied by the formation of H2O2 in the inflamed rheumatoid joint.
  • oxygen free radicals are also produced, especially by polymorphonuclear leukocytes (PMN) and macrophages. In any chronic or acute inflammatory disease, PMN and macrophages will produce both O2 " and H2O2.
  • Tuberculosis, psoriasis, systemic lupus erythematosus, other autoimmune diseases, and adult respiratory distress syndrome can also be mentioned as inflammatory diseases with oxidation as a contributor, and many others can be added to this list.
  • Antioxidants have been suggested to be protective against breast cancer and other cancers including those of the brain and liver, as well as to protect against cardiovascular disease and osteoporosis (Wiseman H, Free Radical Res 21(3): 187-94 (1994)). They have been demonstrated to protect model and cellular membranes including the nuclear membrane against potentially carcinogenic free radical intermediates and the products of lipid peroxidation. Severe complications associated with atherosclerosis and its common incidence have focused attention on prevention and therapy of this vascular disease state, possibly through their ability to protect low density lipoproteins (LDL) against oxidative damage (Steinberg D, N Engl J of Med 14:915-924 (1989)).
  • LDL low density lipoproteins
  • this invention provides a method of treating or inhibiting free radical induced disease states by administering an antioxidant amount of equilenin or its pharmaceutically acceptable salt of the 3-sulfate ester, to a mammal in need thereof.
  • this invention provides a process for treating free radical reactions with enzymes, ion channels, structural proteins and membrane lipids in a mammal, which comprises administering equilenin or a pharmaceutically acceptable sulfate ester salt thereof, as a sacrificial substrate, in an amount sufficient to selectively react with and inhibit free radical reaction with the patients enzymes, ion channels, structural proteins or membrane lipids.
  • antioxidant therapy is indicated to be warranted are with cancers, central nervous system disorders, bone disease, aging, inflammatory disorders, peripheral vascular disease, rheumatoid arthritis, autoimmune diseases, respiratory distress, emphysema, prevention of reperfusion injury, viral hepatitis, chronic active hepatitis, tuberculosis, psoriasis, systemic lupus erythematosus, adult respiratory distress syndrome, central nervous system trauma and stroke.
  • treating covers treatment of an existing condition, ameliorating the condition, or providing palliation of the condition and inhibiting includes inhibiting or preventing the progress or development of the condition.
  • Pharmaceutically acceptable salts of equilenin 3-sulfate ester include, but are not limited to, the alkali metal salts, alkaline earth metal salts, ammonium salts, alkylamine salts containing 1-6 carbon atoms or dialkylamine salts containing 1-6 carbon atoms in each alkyl group.
  • the antioxidant properties of equilenin were established in a standard pharmacological test procedure that measured the its ability to inhibit the formation of oxidatively modified low density lipoprotein (LDL) induced by exposure to either Cu++ ions or cultured endothelial cells (Parthasarathy S, Proc Natl Acad Sci USA 86:1046- 1050 (1989)) by the TBARS (thiobarbituric acid reactive substances) method for analysis of free aldehydes (Yagi K., Biochem Med 15:212-216 (1976)).
  • LDL low density lipoprotein
  • porcine aortic endothelial cells were exposed to LDL that had been modified as above, by exposure to Cu++ in the presence and absence of equilenin. Oxidized LDL has been demonstrated to be cytotoxic to endothelial cells, and this process has also been strongly implicated in the atherogenic process. Subsequent to a 24 hr incubation of the cells with the treated LDL, an MTT assay was performed to assess cytotoxicity (Hansen MB, J Immu Methods 119:203- 210 (1989)). This test procedure assesses the percent of cells that are viable (live) in a given assay.
  • equilenin and the pharmaceutically acceptable salts of its sulfate ester are therefore useful as antioxidants, and in the treatment or inhibition of free radical induced disease states.
  • the antioxidants of this invention can be formulated neat or with a pharmaceutical carrier for administration, the proportion of which is determined by the solubility and chemical nature of the compound, chosen route of administration and standard pharmacological practice.
  • the pharmaceutical carrier may be solid or liquid.
  • a solid carrier can include one or more substances which may also act as flavoring agents, lubricants, solubilizers, suspending agents, fillers, glidants, compression aids, binders or tablet-disintegrating agents; it can also be an encapsulating material.
  • the carrier is a finely divided solid which is in admixture with the finely divided active ingredient.
  • the active ingredient is mixed with a carrier having the necessary compression properties in suitable proportions and compacted in the shape and size desired. The powders and tablets preferably contain up to 99% of the active ingredient.
  • Suitable solid carriers include, for example, calcium phosphate, magnesium stearate, talc, sugars, lactose, dextrin, starch, gelatin, cellulose, methyl cellulose, sodium carboxymethyl cellulose, polyvinylpyrrolidine, low melting waxes and ion exchange resins.
  • Liquid carriers are used in preparing solutions, suspensions, emulsions, syrups, elixirs and pressurized compositions.
  • the active ingredient can be dissolved or suspended in a pharmaceutically acceptable liquid carrier such as water, an organic solvent, a mixture of both or pharmaceutically acceptable oils or fats.
  • the liquid carrier can contain other suitable pharmaceutical additives such as solubilizers, emulsifiers, buffers, preservatives, sweeteners, flavoring agents, suspending agents, thickening agents, colors, viscosity regulators, stabilizers or osmo-regulators.
  • suitable examples of liquid carriers for oral and parenteral administration include water (partially containing additives as above, e.g.
  • cellulose derivatives preferably sodium carboxymethyl cellulose solution
  • alcohols including monohydric alcohols and polyhydric alcohols, e.g. glycols) and their derivatives, lethicins, and oils (e.g. fractionated coconut oil and arachis oil).
  • the carrier can also be an oily ester such as ethyl oleate and isopropyl myristate.
  • Sterile liquid carriers are useful in sterile liquid form compositions for parenteral administration.
  • the liquid carrier for pressurized compositions can be halogenated hydrocarbon or other pharmaceutically acceptable propellant.
  • Liquid pharmaceutical compositions which are sterile solutions or suspensions can be utilized by, for example, intramuscular, intraperitoneal or subcutaneous injection. Sterile solutions can also be administered intravenously.
  • the compounds of this invention can also be administered orally either in liquid or solid composition form.
  • the antioxidants of this invention may be administered rectally in the form of a conventional suppository.
  • the antioxidants of this invention may be formulated into an aqueous or partially aqueous solution, which can then be utilized in the form of an aerosol.
  • the compounds of this invention may also be administered transdermally through the use of a transdermal patch containing the active compound and a carrier that is inert to the active compound, is non toxic to the skin, and allows delivery of the agent for systemic absorption into the blood stream via the skin.
  • the carrier may take any number of forms such as creams and ointments, pastes, gels, and occlusive devices.
  • the creams and ointments may be viscous liquid or semisolid emulsions of either the oil-in-water or water-in-oil type.
  • Pastes comprised of absorptive powders dispersed in petroleum or hydrophilic petroleum containing the active ingredient may also be suitable.
  • a variety of occlusive devices may be used to release the active ingredient into the blood stream such as a semipermiable membrane covering a reservoir containing the active ingredient with or without a carrier, or a matrix containing the active ingredient. Other occlusive devices are known in the literature.
  • antioxidants of this invention may be employed as a solution, cream, or lotion by formulation with pharmaceutically acceptable vehicles containing 0.1 - 5 percent, preferably 2%, of active compound which may be administered to a fungally affected area.
  • the dosage requirements vary with the particular compositions employed, the route of administration, the severity of the symptoms presented and the particular subject being treated. Based on the results obtained in the standard pharmacological test procedures, projected daily dosages of active compound would be 0.02 ⁇ g/kg - 500 ⁇ g kg. Treatment will generally be initiated with small dosages less than the optimum dose of the compound. Thereafter the dosage is increased until the optimum effect under the circumstances is reached; precise dosages for oral, parenteral, nasal, or intrabronchial administration will be determined by the administering physician based on experience with the individual subject treated.
  • the pharmaceutical composition is in unit dosage form, e.g. as tablets or capsules.
  • the composition is sub-divided in unit dose containing appropriate quantities of the active ingredient;
  • the unit dosage forms can be packaged compositions, for example, packeted powders, vials, ampoules, pref ⁇ lled syringes or sachets containing liquids.
  • the unit dosage form can be, for example, a capsule or tablet itself, or it can be the appropriate number of any such compositions in package form.

Abstract

L'invention concerne un procédé d'utilisation de l'équilénine ou d'un sel pharmaceutiquement acceptable d'ester d'équilénine-3-sulfate comme antioxydant.
PCT/US1997/022154 1996-12-10 1997-12-05 Utilisation de l'equilenine comme antioxydant WO1998025626A1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU53707/98A AU743586B2 (en) 1996-12-10 1997-12-05 Use of equilenin as an antioxidant
CA002272087A CA2272087A1 (fr) 1996-12-10 1997-12-05 Utilisation de l'equilenine comme antioxydant
BR9714384-7A BR9714384A (pt) 1996-12-10 1997-12-05 Uso de equeilenina como um antioxidante
NZ336343A NZ336343A (en) 1996-12-10 1997-12-05 Use of equilenin as an antioxidant
EP97950808A EP0944391A1 (fr) 1996-12-10 1997-12-05 Utilisation de l'equilenine comme antioxydant
IL13007197A IL130071A0 (en) 1996-12-10 1997-12-05 Use of equilenin as an antioxidant
JP52680498A JP2001506628A (ja) 1996-12-10 1997-12-05 抗酸化剤としてのエキレニンの使用
HU0000569A HUP0000569A3 (en) 1996-12-10 1997-12-05 Use of equilenin for producing pharmaceutical compositions for the treatment of free radical induced disease states

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76257996A 1996-12-10 1996-12-10
US08/762,579 1996-12-10

Publications (1)

Publication Number Publication Date
WO1998025626A1 true WO1998025626A1 (fr) 1998-06-18

Family

ID=25065472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/022154 WO1998025626A1 (fr) 1996-12-10 1997-12-05 Utilisation de l'equilenine comme antioxydant

Country Status (14)

Country Link
EP (1) EP0944391A1 (fr)
JP (1) JP2001506628A (fr)
KR (1) KR20000069412A (fr)
CN (1) CN1239892A (fr)
AR (1) AR010344A1 (fr)
AU (1) AU743586B2 (fr)
BR (1) BR9714384A (fr)
CA (1) CA2272087A1 (fr)
HU (1) HUP0000569A3 (fr)
IL (1) IL130071A0 (fr)
NZ (1) NZ336343A (fr)
TW (1) TW466115B (fr)
WO (1) WO1998025626A1 (fr)
ZA (1) ZA9711052B (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000059922A1 (fr) * 1999-03-30 2000-10-12 Jenapharm Gmbh & Co. Kg Derives de 14,15-alpha-methylene equilenine, procedes permettant de les preparer et medicaments les contenant
US6271221B1 (en) * 1996-12-10 2001-08-07 American Home Products Corporation Use of equilenin as an antioxidant
EP1461025A1 (fr) * 2001-12-10 2004-09-29 Du, Yansheng, Dr. Traitement de troubles neurodegeneratifs et cardiovasculaires

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108958639B (zh) 2017-05-19 2021-07-06 华邦电子股份有限公司 快闪存储器存储装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4154820A (en) * 1976-02-23 1979-05-15 Akzona Incorporated Compositions containing alkali metal sulfate salts of conjugated estrogens and antioxidants as stabilizers
EP0240717A2 (fr) * 1986-03-04 1987-10-14 Board of Regents of the University of Nebraska Prévention du carcinome du sein
US4937238A (en) * 1986-03-04 1990-06-26 The Board Of Regents Of The University Of Nebraska Prevention of mammary carcinoma
US5545635A (en) * 1995-05-23 1996-08-13 Eli Lilly And Company Inhibiting bone loss with equilenin

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4154820A (en) * 1976-02-23 1979-05-15 Akzona Incorporated Compositions containing alkali metal sulfate salts of conjugated estrogens and antioxidants as stabilizers
EP0240717A2 (fr) * 1986-03-04 1987-10-14 Board of Regents of the University of Nebraska Prévention du carcinome du sein
US4937238A (en) * 1986-03-04 1990-06-26 The Board Of Regents Of The University Of Nebraska Prevention of mammary carcinoma
US5545635A (en) * 1995-05-23 1996-08-13 Eli Lilly And Company Inhibiting bone loss with equilenin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
REIS S.E., ET AL.: "Women's hearts are different", CURRENT PROBLEMS IN OBSTETRICS, GYNECOLOGY AND FERTILITY, vol. 20, no. 3, 1997, pages 72 - 92, XP002062634 *
SUBBIAH M.T.R., KESSEL B. ET AL.: "Antioxidant potential of specific estrogens on lipid peroxidation", J. CLIN. ENDOCRINOL. METAB., vol. 77, no. 4, 1993, pages 1095 - 1097, XP002062633 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271221B1 (en) * 1996-12-10 2001-08-07 American Home Products Corporation Use of equilenin as an antioxidant
WO2000059922A1 (fr) * 1999-03-30 2000-10-12 Jenapharm Gmbh & Co. Kg Derives de 14,15-alpha-methylene equilenine, procedes permettant de les preparer et medicaments les contenant
US6753326B1 (en) 1999-03-30 2004-06-22 Jenapharm Gmbh & Co. Kg 14, 15-.α.-methylene equilenine derivatives, methods for producing the same and medicaments containing them
EP1461025A1 (fr) * 2001-12-10 2004-09-29 Du, Yansheng, Dr. Traitement de troubles neurodegeneratifs et cardiovasculaires
EP1461025B1 (fr) * 2001-12-10 2011-07-27 Du, Yansheng, Dr. Traitement de la maladie de parkinson avec epac

Also Published As

Publication number Publication date
BR9714384A (pt) 2000-05-16
ZA9711052B (en) 1999-06-09
CA2272087A1 (fr) 1998-06-18
NZ336343A (en) 2000-11-24
KR20000069412A (ko) 2000-11-25
TW466115B (en) 2001-12-01
HUP0000569A3 (en) 2000-11-28
JP2001506628A (ja) 2001-05-22
AU5370798A (en) 1998-07-03
HUP0000569A2 (hu) 2000-10-28
CN1239892A (zh) 1999-12-29
AR010344A1 (es) 2000-06-07
IL130071A0 (en) 2000-02-29
AU743586B2 (en) 2002-01-31
EP0944391A1 (fr) 1999-09-29

Similar Documents

Publication Publication Date Title
WO1998032763A1 (fr) Steroides inhibiteurs de l'oestrone sulfatase, compositions pharmaceutiques associees et procedes d'utilisation
AU743586B2 (en) Use of equilenin as an antioxidant
AU703814B2 (en) Antioxidant
US6649779B2 (en) Estrenes
AU743519B2 (en) 17alpha-dihydroequilenin for use as a medical antioxidant
US6271221B1 (en) Use of equilenin as an antioxidant
AU744098B2 (en) Use of 17beta-dihydroequilenin as an antioxidant
EP0981349B1 (fr) 5-alpha-pregnan-3-beta-ol-20-one sulfate pour le traitement des cancers et des maladies du snc
US7022688B1 (en) Use of 17β-dihydroequilenin as an antioxidant
MXPA96006009A (en) Use of 8,9-dehydroestrone as an antioxide
PT1490392E (pt) Quinóis esteroidais como pró-fármacos de antioxidantes
US6395723B2 (en) Method of providing coginition enhancement with 5α-pregnan-3β-ol-20-one sulfate

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 97180448.6

Country of ref document: CN

AK Designated states

Kind code of ref document: A1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase

Ref document number: 2272087

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 1998 526804

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: PA/a/1999/005311

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 1019997005181

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 53707/98

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1997950808

Country of ref document: EP

Ref document number: 336343

Country of ref document: NZ

WWP Wipo information: published in national office

Ref document number: 1997950808

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1019997005181

Country of ref document: KR

WWG Wipo information: grant in national office

Ref document number: 53707/98

Country of ref document: AU

WWR Wipo information: refused in national office

Ref document number: 1019997005181

Country of ref document: KR

WWW Wipo information: withdrawn in national office

Ref document number: 1997950808

Country of ref document: EP