WO1998006084A1 - Methode a technique analytique de code d'acide nucleique utilisee dans des etiquettes infalsifiables - Google Patents

Methode a technique analytique de code d'acide nucleique utilisee dans des etiquettes infalsifiables Download PDF

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Publication number
WO1998006084A1
WO1998006084A1 PCT/CN1997/000078 CN9700078W WO9806084A1 WO 1998006084 A1 WO1998006084 A1 WO 1998006084A1 CN 9700078 W CN9700078 W CN 9700078W WO 9806084 A1 WO9806084 A1 WO 9806084A1
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
dna
label
solid
counterfeiting
Prior art date
Application number
PCT/CN1997/000078
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English (en)
Chinese (zh)
Inventor
Su Han
Xinghua Guo
Xueyuan Jin
Xiaozhou Shen
Original Assignee
Beijing Sanzhu Xinda Biological Probe Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Sanzhu Xinda Biological Probe Co., Ltd. filed Critical Beijing Sanzhu Xinda Biological Probe Co., Ltd.
Priority to AU37648/97A priority Critical patent/AU3764897A/en
Publication of WO1998006084A1 publication Critical patent/WO1998006084A1/fr

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Classifications

    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09F—DISPLAYING; ADVERTISING; SIGNS; LABELS OR NAME-PLATES; SEALS
    • G09F3/00—Labels, tag tickets, or similar identification or indication means; Seals; Postage or like stamps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Definitions

  • the invention applies the nucleic acid code analysis technology to the invisible anti-counterfeiting mark of goods or other objects, and belongs to the technical field of cryptography methods.
  • anti-counterfeiting marks are mostly products of physical or chemical nature, such as laser holograms, optical discoloration films, image scramblers, thermally variable inks, magnetic stripes, and software.
  • anti-counterfeiting products made by biotechnology have begun to appear internationally. These products mainly adopt the principle of antigen-antibody reaction, which is more accurate and sensitive than ordinary anti-counterfeiting products and methods.
  • the antigen antibody is a protein, which has poor stability, and is easy to inactivate especially in a high temperature environment, which will reduce the sensitivity and reliability of anti-counterfeiting.
  • the antigen-antibody response changes little, and once one of the components is known, it is easy to be counterfeited.
  • the purpose of the present invention is to overcome the defect that the existing mark is easy to be counterfeited in the technical field of anti-counterfeiting, and to design an invisible labeling method in which the nucleic acid code analysis technology in genetic engineering is applied to anti-counterfeiting.
  • the present invention is based on the principle of genetic engineering, and proposes a new invisible anti-counterfeiting design and technology, the core of which is the biological macromolecule-nucleic acid.
  • the genetic material (genes) of all life are nucleic acids, that is, deoxyribonucleic acid (DNA) or ribonucleic acid (RNA).
  • Nucleic acids are composed of four kinds of alternating arrangements of bases. The difference in the arrangement and combination of these forms of life on earth There are many types. A DNA with only 1 000 base pairs (1 kb) may have 10 6 ° two permutations and combinations.
  • Species existing in nature are numerous security genome length of each species is generally greater than 104 to 1 0 6 kb. If a nucleic acid with a length of 1 kb is set as an anti-counterfeiting mark, the available nucleic acid code is 10 6 X (1 0 4-1 0 6 ) ⁇ IO 1 0- ] 0 1 2 as many. Therefore, biodiversity provides an inexhaustible source of anti-counterfeiting technology. With the huge amount of information such as DNA, hundreds of millions of passwords can be used for anti-counterfeiting designs.
  • the present invention is a nucleic acid fragments preselected some (mainly DNA fragments, because very stable) in an amount (as little to 10--8 g) was dissolved in the solution and applied to a variety of solid support, After evaporative drying, the nucleic acid fragment is attached to the carrier.
  • the solid support can be a variety of materials, such as paper or films made of various natural or synthetic fibers, porous particles or powders, natural or synthetic leather, plastics, or various organic or inorganic polymers and resins, Solid paraffin, natural or synthetic inorganic substances such as ceramics, glass, crystal, metal, diatomaceous earth, etc.
  • the nucleic acid fragment can also be directly added to the liquid product as a solution as an invisible anti-counterfeiting mark, or the nucleic acid fragment can also be placed in a variety of liquid carriers such as ink, ink, paint or adhesive to form an anti-counterfeiting mark. Adhere to merchandise or other packaging.
  • a protective layer is added on the surface of the carrier containing the nucleic acid fragment, for example, a plastic film,
  • the invisible anti-counterfeit of nucleic acid made of fucose and the like can be fixed on a commodity or an object or on a package and an accessory.
  • nucleic acid anti-counterfeiting mark When inspection is required, remove the anti-counterfeiting mark, remove the protective layer, and dissolve the nucleic acid fragments attached to the carrier with a buffer solution.
  • Detecting personnel are aware of the types of nucleic acid anti-counterfeiting marks on goods or objects, and can use specific nucleic acid molecular hybridization or polymerase chain reaction (PCR) specific primers for detection. If the hybridization signal or PCR product with the correct length (PCR specific primer method) can be displayed, it can be judged that the detected commodity or object is genuine, otherwise it is a fake or fake.
  • PCR specific primer method polymerase chain reaction
  • nucleic acid code is colorless and hard to see with the naked eye.
  • code comes from the huge gene bank in nature. Even if you know the code of the gene but do n’t know which one, the counterfeiter cannot decipher and forge it. For inspectors, as long as they are tested with a dedicated analysis method, the authenticity is clear at a glance.
  • nucleic acid codes can be easily designed as anti-counterfeiting markers, each of which has its own corresponding probes and primers, which are mutually exclusive. Therefore, the nucleic acid code can provide a unique anti-counterfeiting marking system for a variety of goods and objects.
  • the D N A password is stable, especially the dry D N A is stored for a long time, it is not easy to decompose, and it is suitable for the security of some long-term storage objects.
  • the detection and detection method used to distinguish the authenticity of the object is an inseparable technical content of the nucleic acid code anti-counterfeiting method.
  • the present invention uses a specific nucleic acid molecule hybridization method or a polymerase chain reaction (P C R) method for detection and detection.
  • Figure 1 is a schematic diagram of nucleic acid molecular hybridization method to verify the authenticity of DNA anti-counterfeiting marks.
  • A is blank (no DNA);
  • B is other non-specific DNA, 10 micrograms;
  • C is C ⁇ T coding gene, 0.0 2 microgram.
  • FIG. 2 is a schematic diagram of the P C R method to verify the authenticity of the D N A anti-counterfeiting mark.
  • A is blank (without D N A template);
  • B is non-specific D N A, 1 microgram;
  • C is lambda phage D N A template '. 0 1 microgram
  • Example 1 The reliability and specificity of anti-counterfeiting marks depends on accurate methods of forgery detection.
  • the present embodiment uses a method of hybridizing nucleic acid molecules.
  • DN A is a double-helix structure of two nucleotide strands that are coiled together in opposite directions.
  • the two nucleotide chains depend on the hydrogen bonds on the four bases of adenine (A) and thymine (T) and guanine (G) and cytosine (C). Therefore, the binding between DNA-DNA or DNA-RN A chains is called "molecular hybridization", and its stability depends entirely on the strict complementarity between the double-stranded bases.
  • a and T are complementary (in DNA-RNA) hybrids
  • a and U are complementary
  • G and C are complementary.
  • Single-stranded DNA or RNA molecular probes hybridize only to their DNA strands that are complementary to their homology.
  • Solid-phase hybridization The denatured DNA membrane is placed in the hybridization solution, and a nucleic acid probe (isotopic probe or non-isotopic chemiluminescence probe) is added to the hybridization solution for 4 hours or overnight in a 65'C hybridization solution. Washing to remove non-specifically bound probes, and finally exposing them with X-ray film;
  • P CR Polymerase chain reaction
  • T aq DNA polymerase thermostable bacteria DNA polymerase
  • the PCR reaction consists of a specific one or a pair of primers and a template DNA. A very small amount of DNA is placed on the mark of the anti-counterfeit object. The DNA is removed during the detection and dissolved in a certain amount of buffer solution.
  • T aq DA polymerase and other reaction solutions Use PCR to perform amplification reactions.
  • the DNA amplification reaction is the key to anti-counterfeiting technology. Enzymes and other methods that can make DNA amplified can also achieve the purpose.
  • a part of the reaction solution was taken out for electrophoresis analysis.
  • the product should be an amplified D N A fragment of a predetermined size, which can be separated by agarose gel electrophoresis, then stained with ethidium bromide, observed and identified under ultraviolet light, and the operation process is briefly described as follows:
  • reaction components (1) DNA template (about 0. () micrograms), (2) one or more specific primers, (3) T aq DNA polymerase or other DNA polymerase; (4) four Deoxynucleoside triphosphates (d AT P, d TTP, d CT P, and d GT P), (5) a reaction buffer, 2. Reaction temperature and procedure:
  • Electrophoretic separation Take 10 microliters of the reaction solution, place it in a 2% agarose gel (containing ethidium bromide dye), and perform electrophoresis with a DC voltage of 100 V. After electrophoresis, remove the agarose gel and place it in Observe and photograph under a UV light of 2 50-3 0 0 m.
  • Embodiment 2 is only a general example, and is not a fixed operation procedure. In practice, it often varies with the design of the D N A die and the primer, otherwise the expected results cannot be achieved. This strict operating condition is also very beneficial to anti-counterfeiting, because the anti-counterfeit cannot know the reaction conditions and cannot decode the password.
  • the nucleic acid anti-counterfeiting label of the present invention has imitation, reliability, diversity, stability, and wide applicability, and has high industrial applicability.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Pour venir à bout des inconvénients que procurent les étiquettes déjà falsifiées, la présente invention propose une méthode à technique analytique de code d'acide nucléique utilisée dans une étiquette infalsifiable invisible de marchandises ou d'articles, qui appartient au domaine de la technique des codes. Un fragment d'acide nucléique sélectionné (spécialement un fragment d'ADN) dissous dans une solution est appliqué sur divers supports solides, puis fixé au support après avoir été séché. En tant qu'étiquette infalsifiable, il est directement étiqueté sur diverses marchandises solides, articles ou emballages et leurs accessoires. L'acide nucléique peut également être directement incorporé dans les marchandises liquides ou dans divers supports liquides pour former une étiquette infalsifiable invisible. La méthode de détection consiste en des dosages par sonde d'hybridation de molécule d'acide nucléique ou des dosages par amorces spécifiques de réaction en chaîne de polyase.
PCT/CN1997/000078 1996-08-02 1997-08-01 Methode a technique analytique de code d'acide nucleique utilisee dans des etiquettes infalsifiables WO1998006084A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU37648/97A AU3764897A (en) 1996-08-02 1997-08-01 A method of nucleic acid code analystic technique used in falseproof label

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN96109232.7 1996-08-02
CN96109232A CN1148227A (zh) 1996-08-02 1996-08-02 核酸密码分析技术应用于防伪的方法

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Publication Number Publication Date
WO1998006084A1 true WO1998006084A1 (fr) 1998-02-12

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CN (1) CN1148227A (fr)
AU (1) AU3764897A (fr)
WO (1) WO1998006084A1 (fr)

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2775693A1 (fr) * 1998-03-03 1999-09-10 Genolife Utilisation d'un polymere nucleique comme marqueur d'authenticite de produits et moyens mis en oeuvre pour sa revelation
WO2000059731A1 (fr) * 1999-03-31 2000-10-12 Norbert Hampp Procede et preparation de marquage photochrome et/ou de garantie de l'authenticite d'objets
WO2002093504A2 (fr) * 2001-05-11 2002-11-21 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Fil de securite pour le marquage infalsifiable d'objets
EP1394544A1 (fr) * 2002-08-30 2004-03-03 Biowell Technology Inc. Mèthode de mélange d'acides nucléiques dans un milieu insoluble dans l'eau, et son utilisation
WO2006127558A2 (fr) * 2005-05-20 2006-11-30 Applied Dna Sciences, Inc Systeme et methode pour authentifier plusieurs composants associes a un produit particulier
GB2434570A (en) * 2006-01-31 2007-08-01 Alexander Peter Mackay Applying DNA as an item label
US8124333B2 (en) 2003-04-16 2012-02-28 APDN, Inc. Methods for covalent linking of optical reporters
US8372648B2 (en) 2003-04-16 2013-02-12 APDN (B.V.I.), Inc. Optical reporter compositions
US8415164B2 (en) 2003-04-16 2013-04-09 Apdn (B.V.I.) Inc. System and method for secure document printing and detection
US8415165B2 (en) 2003-04-16 2013-04-09 APDN (B.V.I.), Inc. System and method for authenticating sports identification goods
US8420400B2 (en) 2003-04-16 2013-04-16 APDN (B.V.I.), Inc. System and method for authenticating tablets
US8426216B2 (en) 2003-04-16 2013-04-23 APDN (B.V.I.), Inc. Methods for authenticating articles with optical reporters
US8669079B2 (en) 2008-11-12 2014-03-11 Cara Therapeutics, Inc. Methods for genetic analysis of textiles made of Gossypium barbadense and Gossypium hirsutum cotton
US8940485B2 (en) 2008-11-12 2015-01-27 Apdn (B.V.I.) Inc. Methods for genotyping mature cotton fibers and textiles
US9297032B2 (en) 2012-10-10 2016-03-29 Apdn (B.V.I.) Inc. Use of perturbants to facilitate incorporation and recovery of taggants from polymerized coatings
US9790538B2 (en) 2013-03-07 2017-10-17 Apdn (B.V.I.) Inc. Alkaline activation for immobilization of DNA taggants
US9904734B2 (en) 2013-10-07 2018-02-27 Apdn (B.V.I.) Inc. Multimode image and spectral reader
US9919512B2 (en) 2012-10-10 2018-03-20 Apdn (B.V.I.) Inc. DNA marking of previously undistinguished items for traceability
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
US10047282B2 (en) 2014-03-18 2018-08-14 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10519605B2 (en) 2016-04-11 2019-12-31 APDN (B.V.I.), Inc. Method of marking cellulosic products
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10760182B2 (en) 2014-12-16 2020-09-01 Apdn (B.V.I.) Inc. Method and device for marking fibrous materials
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014026299A (ja) * 2010-11-11 2014-02-06 Angel Playing Cards Co Ltd 遊戯用代用貨幣および遊戯用代用貨幣の判定システム

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US3861886A (en) * 1968-11-13 1975-01-21 Melpar Inc Material identification coding methods and systems
US3772200A (en) * 1971-04-30 1973-11-13 Minnesota Mining & Mfg Method of tagging with microparticles
US4390452A (en) * 1979-08-20 1983-06-28 Minnesota Mining & Manufacturing Company Microparticles with visual identifying means
US4387112A (en) * 1980-10-23 1983-06-07 Blach Rodney J Article identification process and articles for practice thereof
EP0111340A2 (fr) * 1982-12-13 1984-06-20 Genzyme Corporation Procédé de liaison de l'acide nucléique à un support
WO1987006383A1 (fr) * 1986-04-09 1987-10-22 Biotal Limited Marquage d'articles qu'on desire authentifier
CN1037969A (zh) * 1988-02-02 1989-12-13 国际壳牌研究有限公司 通过免疫测定法检测化学物质
WO1989007272A1 (fr) * 1988-02-08 1989-08-10 Shell Internationale Research Maatschappij B.V. Marquage de produits chimiques pour en etablir l'identite et l'origine

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2775693A1 (fr) * 1998-03-03 1999-09-10 Genolife Utilisation d'un polymere nucleique comme marqueur d'authenticite de produits et moyens mis en oeuvre pour sa revelation
WO2000059731A1 (fr) * 1999-03-31 2000-10-12 Norbert Hampp Procede et preparation de marquage photochrome et/ou de garantie de l'authenticite d'objets
US6616964B1 (en) 1999-03-31 2003-09-09 Norbert Hampp Method and preparation for the photochromic marking and/or for securing the authenticity of objects
WO2002093504A2 (fr) * 2001-05-11 2002-11-21 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Fil de securite pour le marquage infalsifiable d'objets
WO2002093504A3 (fr) * 2001-05-11 2004-03-18 November Ag Molekulare Medizin Fil de securite pour le marquage infalsifiable d'objets
EP1394544A1 (fr) * 2002-08-30 2004-03-03 Biowell Technology Inc. Mèthode de mélange d'acides nucléiques dans un milieu insoluble dans l'eau, et son utilisation
US8415165B2 (en) 2003-04-16 2013-04-09 APDN (B.V.I.), Inc. System and method for authenticating sports identification goods
US8124333B2 (en) 2003-04-16 2012-02-28 APDN, Inc. Methods for covalent linking of optical reporters
US8372648B2 (en) 2003-04-16 2013-02-12 APDN (B.V.I.), Inc. Optical reporter compositions
US8415164B2 (en) 2003-04-16 2013-04-09 Apdn (B.V.I.) Inc. System and method for secure document printing and detection
US9005985B2 (en) 2003-04-16 2015-04-14 Apdn (B.V.I.) Inc. Optical reporter compositions
US8420400B2 (en) 2003-04-16 2013-04-16 APDN (B.V.I.), Inc. System and method for authenticating tablets
US8426216B2 (en) 2003-04-16 2013-04-23 APDN (B.V.I.), Inc. Methods for authenticating articles with optical reporters
WO2006127558A3 (fr) * 2005-05-20 2009-05-07 Applied Dna Sciences Inc Systeme et methode pour authentifier plusieurs composants associes a un produit particulier
WO2006127558A2 (fr) * 2005-05-20 2006-11-30 Applied Dna Sciences, Inc Systeme et methode pour authentifier plusieurs composants associes a un produit particulier
GB2434570A (en) * 2006-01-31 2007-08-01 Alexander Peter Mackay Applying DNA as an item label
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
US8940485B2 (en) 2008-11-12 2015-01-27 Apdn (B.V.I.) Inc. Methods for genotyping mature cotton fibers and textiles
US8669079B2 (en) 2008-11-12 2014-03-11 Cara Therapeutics, Inc. Methods for genetic analysis of textiles made of Gossypium barbadense and Gossypium hirsutum cotton
US9290819B2 (en) 2008-11-12 2016-03-22 Apdn (B.V.I.) Inc. Methods for genotyping mature cotton fibers and textiles
US9297032B2 (en) 2012-10-10 2016-03-29 Apdn (B.V.I.) Inc. Use of perturbants to facilitate incorporation and recovery of taggants from polymerized coatings
US9919512B2 (en) 2012-10-10 2018-03-20 Apdn (B.V.I.) Inc. DNA marking of previously undistinguished items for traceability
US9790538B2 (en) 2013-03-07 2017-10-17 Apdn (B.V.I.) Inc. Alkaline activation for immobilization of DNA taggants
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
US9904734B2 (en) 2013-10-07 2018-02-27 Apdn (B.V.I.) Inc. Multimode image and spectral reader
US10282480B2 (en) 2013-10-07 2019-05-07 Apdn (B.V.I) Multimode image and spectral reader
US10047282B2 (en) 2014-03-18 2018-08-14 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10760182B2 (en) 2014-12-16 2020-09-01 Apdn (B.V.I.) Inc. Method and device for marking fibrous materials
US10519605B2 (en) 2016-04-11 2019-12-31 APDN (B.V.I.), Inc. Method of marking cellulosic products
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication

Also Published As

Publication number Publication date
CN1148227A (zh) 1997-04-23
AU3764897A (en) 1998-02-25

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