WO1997021824A3 - Procede visant a obtenir un surnageant vectoriel retroviral a haute efficacite de transduction - Google Patents

Procede visant a obtenir un surnageant vectoriel retroviral a haute efficacite de transduction Download PDF

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Publication number
WO1997021824A3
WO1997021824A3 PCT/US1996/019904 US9619904W WO9721824A3 WO 1997021824 A3 WO1997021824 A3 WO 1997021824A3 US 9619904 W US9619904 W US 9619904W WO 9721824 A3 WO9721824 A3 WO 9721824A3
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WO
WIPO (PCT)
Prior art keywords
transduction efficiency
retroviral vector
high transduction
vector supernatant
obtaining
Prior art date
Application number
PCT/US1996/019904
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English (en)
Other versions
WO1997021824A2 (fr
Inventor
Richard J Rigg
Jonathan S Dando
Sean P Forestell
Ernst Bohnlein
Original Assignee
Richard J Rigg
Jonathan S Dando
Sean P Forestell
Ernst Bohnlein
Systemix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/572,959 external-priority patent/US5910434A/en
Application filed by Richard J Rigg, Jonathan S Dando, Sean P Forestell, Ernst Bohnlein, Systemix Inc filed Critical Richard J Rigg
Priority to AU13337/97A priority Critical patent/AU1333797A/en
Priority to EP96944812A priority patent/EP0871753A2/fr
Priority to JP9522241A priority patent/JP2000501614A/ja
Publication of WO1997021824A2 publication Critical patent/WO1997021824A2/fr
Publication of WO1997021824A3 publication Critical patent/WO1997021824A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)

Abstract

L'invention se rapporte à des procédés visant à obtenir des surnageants rétroviraux à haute efficacité de transduction.
PCT/US1996/019904 1995-12-15 1996-12-13 Procede visant a obtenir un surnageant vectoriel retroviral a haute efficacite de transduction WO1997021824A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU13337/97A AU1333797A (en) 1995-12-15 1996-12-13 Method for obtaining retroviral vector supernatant having high transduction efficiency
EP96944812A EP0871753A2 (fr) 1995-12-15 1996-12-13 Procede visant a obtenir un surnageant vectoriel retroviral a haute efficacite de transduction
JP9522241A JP2000501614A (ja) 1995-12-15 1996-12-13 高形質導入効率を有するレトロウイルスベクター上清を得るための方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US57294395A 1995-12-15 1995-12-15
US08/572,943 1995-12-15
US08/572,959 1995-12-15
US08/572,959 US5910434A (en) 1995-12-15 1995-12-15 Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant

Publications (2)

Publication Number Publication Date
WO1997021824A2 WO1997021824A2 (fr) 1997-06-19
WO1997021824A3 true WO1997021824A3 (fr) 1997-09-04

Family

ID=27075989

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/019904 WO1997021824A2 (fr) 1995-12-15 1996-12-13 Procede visant a obtenir un surnageant vectoriel retroviral a haute efficacite de transduction

Country Status (5)

Country Link
EP (1) EP0871753A2 (fr)
JP (1) JP2000501614A (fr)
AU (1) AU1333797A (fr)
CA (1) CA2238434A1 (fr)
WO (1) WO1997021824A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994134A (en) * 1998-05-04 1999-11-30 Canji, Inc. Viral production process
US6790614B1 (en) 1999-11-19 2004-09-14 Novartis Ag Selectable cell surface marker genes
CN101506355B (zh) 2004-09-24 2012-06-27 成血管细胞系统公司 多能扩增间充质前体细胞子代（memp）及其应用
EP2360242A1 (fr) 2004-09-24 2011-08-24 Angioblast Systems, Inc. Procédé d'amélioration de la prolifération et/ou survie des cellules de précurseurs mesenchymataux (MPC)
KR101682046B1 (ko) 2005-04-12 2016-12-02 메소블라스트, 아이엔씨. 조직 비특이적인 알카리 포스파타제에 의한 다분화성 성체 세포의 분리
US8609411B2 (en) 2007-05-04 2013-12-17 Whitehead Institute For Biomedical Research Ex vivo expansion of human hematopoietic stem cells
CA2694107C (fr) 2007-08-06 2018-03-27 Angioblast Systems, Inc. Procedes permettant de generer, reparer et/ou preserver du tissu conjonctif in vivo
EP2574677B1 (fr) * 2007-12-27 2017-09-13 Baxalta GmbH Procédés de culture de cellules
SG10201601279SA (en) 2008-08-18 2016-03-30 Mesoblast Inc Monoclonal Antibody STRO-4
ES2671932T3 (es) 2012-08-01 2018-06-11 United Therapeutics Corporation Tratamiento de hipertensión arterial pulmonar con células madre mesenquimatosas
ES2869701T3 (es) 2012-08-01 2021-10-25 United Therapeutics Corp Tratamiento de hipertensión arterial pulmonar con células progenitoras endoteliales tratadas con prostaciclina
ES2697573T3 (es) 2013-01-09 2019-01-25 United Therapeutics Corp Tratamiento de la vasculopatía con prostaciclina y células madre mesenquimatosas
EP3546485A1 (fr) 2013-05-10 2019-10-02 Whitehead Institute for Biomedical Research Production in vitro de globules rouges avec des protéines pouvant être médiées par une sortase
JP6730193B2 (ja) * 2014-04-25 2020-07-29 ジェネトン 高ビリルビン血症の処置
HUE050264T2 (hu) 2014-11-05 2020-11-30 Juno Therapeutics Inc Eljárások transzdukálásra és sejtfeldolgozásra
KR102606101B1 (ko) 2016-10-24 2023-11-27 유나이티드 쎄러퓨틱스 코포레이션 트레프로스티닐에 의한 msc 면역조절 특성의 향상
JP7337784B2 (ja) 2017-10-16 2023-09-04 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 灌流バイオリアクタ及び関連する使用方法
WO2024075819A1 (fr) * 2022-10-07 2024-04-11 Ａｇｃ株式会社 Procédé de production de vecteur viral utilisant une cellule

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007150A1 (fr) * 1988-02-05 1989-08-10 The Trustees Of Columbia University In The City Of Lignees de cellules de conservation de retrovirus et leurs procedes d'utilisation
WO1994019478A1 (fr) * 1993-02-22 1994-09-01 The Rockefeller University Production de retrovirus exempts d'auxiliaires, a titre eleve par transfection transitoire
WO1996004934A1 (fr) * 1994-08-17 1996-02-22 Genetic Therapy Inc. Vecteurs retroviraux produits par des lignees de cellules productrices resistantes a la lyse par le serum humain

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007150A1 (fr) * 1988-02-05 1989-08-10 The Trustees Of Columbia University In The City Of Lignees de cellules de conservation de retrovirus et leurs procedes d'utilisation
WO1994019478A1 (fr) * 1993-02-22 1994-09-01 The Rockefeller University Production de retrovirus exempts d'auxiliaires, a titre eleve par transfection transitoire
WO1996004934A1 (fr) * 1994-08-17 1996-02-22 Genetic Therapy Inc. Vecteurs retroviraux produits par des lignees de cellules productrices resistantes a la lyse par le serum humain

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
EVANS L H ET AL: "A NEUTRALIZABLE EPITOPE COMMON TO THE ENVELOPE GLYCOPROTEINS OF ECOTROPIC, POLYTROPIC, XENOTROPIC, AND AMPHOTROPIC MURINE LEUKEMIA VIRUSES", JOURNAL OF VIROLOGY, vol. 64, no. 12, December 1990 (1990-12-01), pages 6176 - 6183, XP000653216 *
FORESTELL S P ET AL: "RETROVIRAL END-POINT TITER IS NOT PREDICTIVE OF GENE TRANSFER EFFICIENCY: IMPLICATIONS FOR VECTOR PRODUCTION", GENE THERAPY, vol. 2, no. 10, 19 December 1995 (1995-12-19), pages 723 - 730, XP000653184 *
MILLER A D: "RETROVIRUS PACKAGING CELLS", HUMAN GENE THERAPY, vol. 1, 1990, pages 5 - 14, XP000653181 *
MILLER D ET AL: "REDESIGN OF RETROVIRUS PACKAGING CELL LINES TO AVOID RECOMBINATION LEADING TO HELPER VIRUS PRODUCTION", MOLECULAR AND CELLULAR BIOLOGY, vol. 6, no. 8, August 1986 (1986-08-01), pages 2895 - 2902, XP000605368 *
RIGG R J ET AL: "A NOVEL HUMAN AMPHOTROPIC PACKAGING CELL LINE: HIGH TILTER, COMPLEMENT RESISTANCE, AND IMPROVED SAFETY", VIROLOGY, vol. 218, no. 1, 1 January 1996 (1996-01-01), pages 290 - 295, XP000570621 *
SONEOKA Y ET AL: "A TRANSIENT THREE-PLASMID EXPRESSION SYSTEM FOR THE PRODUCTION OF HIGH TITER RETROVIRAL VECTORS", NUCLEIC ACIDS RESEARCH, vol. 23, no. 4, 25 February 1995 (1995-02-25), pages 628 - 633, XP000569533 *

Also Published As

Publication number Publication date
CA2238434A1 (fr) 1997-06-19
EP0871753A2 (fr) 1998-10-21
AU1333797A (en) 1997-07-03
WO1997021824A2 (fr) 1997-06-19
JP2000501614A (ja) 2000-02-15

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