WO1996030274A1 - Fermeture de tube - Google Patents

Fermeture de tube Download PDF

Info

Publication number
WO1996030274A1
WO1996030274A1 PCT/US1996/004347 US9604347W WO9630274A1 WO 1996030274 A1 WO1996030274 A1 WO 1996030274A1 US 9604347 W US9604347 W US 9604347W WO 9630274 A1 WO9630274 A1 WO 9630274A1
Authority
WO
WIPO (PCT)
Prior art keywords
closure
culture
tube
endcap
culture tube
Prior art date
Application number
PCT/US1996/004347
Other languages
English (en)
Other versions
WO1996030274A9 (fr
Inventor
Kevin Theodore Lampe
Original Assignee
Smithkline Beecham Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Beecham Corporation filed Critical Smithkline Beecham Corporation
Publication of WO1996030274A1 publication Critical patent/WO1996030274A1/fr
Publication of WO1996030274A9 publication Critical patent/WO1996030274A9/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis

Definitions

  • the present invention relates to a closure for a culture tube.
  • the present invention is directed to a gas-permeable, liquid-resistant closure.
  • vi tro In order to culture cells in vi tro, chemically defined media containing the necessary nutrients, vitamins, ions and other essential metabolites, is required to provide the proper environment for the metabolism, growth and function of the cells, tissue or organisms.
  • pH is maintained in vi tro through bicarbonate buffering.
  • NaHCO 3 is present in the media and cells are cultured in a controlled atmosphere of CO 2 and air at ambient
  • Culture vessels such as flasks, tubes, dishes and multiwell plates must be constructed to allow for exchange of the controlled atmosphere with the liquid media.
  • the neck of cell culture flasks and tubes is typically threaded and supplied with a cap.
  • the flasks and tubes do not allow for small volume cell culture.
  • the Falcon vented tissue culture flask (Falcon Labware catalog nos. 3108, 3109, 3110, 3111 and 3112). These devices utilize a 0.2 um hydrophobic filter membrane incorporated into a modified screw cap.
  • the filter membrane acts as a vent and allows for gas exchange while the cap is tightly screwed onto the flask.
  • the small pore nature of the membrane minimizes the possibility of contamination of the culture by excluding bacteria and spores.
  • vented plug seal screw-capped flasks are inadequate for small volume cell culture. Moreover, these devices do not allow for cell sedimentation by centrifugation without a transfer step to a centrifuge tube.
  • U.S. Patent No. 4,763,804 discloses an autoclavable tissue culture container and closure which provides for semi-open positioning of the closure. This device does not allow for mixing the contents of the culture
  • U.S. Patent No. 4,057,168 discloses a vented top for a bacteria culture medium tube.
  • a diaphragm member functions as a one-way check valve which permits the escape of gases but prevents the entry of impurities.
  • U.S. Patent No. 4,271,973 discloses a sterility testing vessel having a closure.
  • the vessel is a plain cylindrical container having a flat bottom and is preferably made of borosilicate glass.
  • the closure is in the form of a one-piece molded cap which houses a filter element held in place by a clip.
  • the filter is disclosed to act as a vent to prevent pressure
  • the vessel volume is disclosed to be about 250 to about 400 ml.
  • microcentrifuge tubes having volumes of 250 ul to 1.5 ml by partially unscrewing or loosely fitting the caps. This, of course, may allow entry of contaminants into the culture and does not allow for constant mixing without risk of spillage or leakage of the tube
  • the present invention relates to a closure for a culture tube.
  • the closure includes an annular endcap having an annular rim for sealing
  • Fig. 1 is a elevational view of a closure in accordance with the present invention
  • Fig. 2 is an enlarged cross-sectional view of the closure of Fig. 1 taken along line 2-2 of Fig. 1; and Fig. 3 is a perspective view of an alternative embodiment of a closure in accordance with the present invention.
  • a closure 10 for an annular culture tube 13 (not shown in Fig. 2).
  • the closure 10 comprises a molded annular endcap 11 having an annular rim 12 for sealing engagement with the culture tube and an endwall 14.
  • the endwall 14 has a top face 16 and a bottom face 18, both of which are perforated by one or more openings 20.
  • Located adjacent to the bottom face 18 of the endwall 14 is a gas-permeable, hydrophobic membrane 22 which is affixed to the entire periphery of the endwall 14 such that there are no gaps between the membrane 22 and endwall 14.
  • the membrane is annular in shape. Affixation of the membrane 22 to the endcap 11 can be accomplished by techniques well known to those skilled in the art such as gluing in place using a cell/tissue culture-compatible chemical adhesive, heat or ultrasonic fusion or mechanical means.
  • the membrane porosity can be varied.
  • the pore size of the membrane 22 allows for unimpeded gas exchange between the interior and exterior of a cell culture tube 13.
  • a membrane which can be sterilized is also preferred.
  • the gas-permeable, hydrophobic membrane has a pore size of 0.2 um or 0.45 um.
  • an autoclavable membrane having a pore size of 0.2 um.
  • Materials for the gas-permeable, hydrophobic membrane can be obtained from, e.g., Millipore
  • Exemplary membranes available from Millipore include Durapore® hydrophobic membrane (0.22 and 0.45 um pore size, autoclavable) and Durapel® hydrophobic membrane (0.2 to 2.0 um pore size).
  • Exemplary membranes available from Gelman Sciences which are autoclavable and hydrophobic include GN-6 Metricel® (0.45 um pore size, made of mixed cellulose esters), TF (PTFE) (0.2, 0.45 and 1.0 um pore size, made of
  • the annular endcap is constructed of a cell culture compatible plastic material, which may or may not permit attachment of cells. Construction of the endcap can be achieved by plastic fabrication techniques well known to those skilled in the art. Preferably, the plastic material used for fabrication is polystyrene,
  • the plastic material is polypropylene.
  • the culture tube can be any annular tube with a volume of up to 50 ml in which biological material such as viruses, bacteria, eukaryotic cells and tissues can be cultured or incubated.
  • the culture tube is a plastic, conical-type microcentrigue tube having a volume of 250, 400, 500, 1500 or 2000 ul, such as those available from Sarstedt, USA/Scientific Plastics, Nunc, S/P, Eppendorf and Brinkmann.
  • the closure of the invention sealably engages the wall of the culture tube.
  • the seal formed is air and liquid tight.
  • the annular rim 12 can incorporate friction-fit means 26 to sealably engage the culture tube 13.
  • Closures employing friction-fit means can be of a press-on or a flip-top type.
  • the annular rim 12 can incorporate thread means 24 to sealably engage the culture tube 13.
  • the closure 10 is sealably engaged to the culture tube.
  • Appropriate culture/incubation media for culture of viruses, bacteria, eukaryotic cells or tissues in suspension or incubation of biological materials are present in the culture tube. If cell culture is desired, appropriate seed materials are also present in the culture tube.
  • the gas-permeability property of the membrane 22 allows for free gas exchange between the culture media within the tube and a defined atmosphere within a cell or tissue culture incubator such that gases within the incubator equilibrate with the culture media thereby maintaining optimal
  • the hydrophobicity of the membrane 22 allows the membrane to resist wetting by liquids inside of or outside of the tube, thereby allowing the contents to be mixed, e.g., continuously on a mechanical mixer, without the possibility of the membrane becoming wet.
  • Continuous mixing causes faster equilibration of the media and provides an advantage in culturing living organisms. See Example, infra .
  • Non-wetting of the membrane prevents possible microbial contamination of the culture as well as the preclusion of gas exchange across the membrane.
  • the small pore size of the membrane prevents biological and non-biological contaminants from entering the tube. Thus, a sterile environment is maintained within the tube.
  • the closure effectively prevented loss of sterility of the media within the tubes for at least 5 days under cell culture conditions (37° C, 95% relative humidity, 5% CO 2 /95% air).
  • the integrity of the membrane was examined microscopically after repeated autoclaving (250° F for 20 min), centrifugation (tested up to 12,000x g) or wetting (with cell culture media), alone or in
  • Tubes with Ca/Mg-free HBSS media were prepared as described above with the exception that solid caps were left in place on one set of tubes, one set of tubes was left uncapped and a closure in accordance with the invention put in place on all other sets.
  • Two sets of tubes capped by a closure of the invention (vent cap) (one set autoclaved) were placed on a gyrating mixer and allowed to mix

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention décrit une fermeture (10) pour tube de culture (13), la fermeture (10) étant perméable aux gaz et résistante aux liquides. Ladite fermeture inclut une membrane (22) hydrophobe et perméable aux gaz.
PCT/US1996/004347 1995-03-31 1996-03-29 Fermeture de tube WO1996030274A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9506709.6 1995-03-31
GBGB9506709.6A GB9506709D0 (en) 1995-03-31 1995-03-31 Tube closure

Publications (2)

Publication Number Publication Date
WO1996030274A1 true WO1996030274A1 (fr) 1996-10-03
WO1996030274A9 WO1996030274A9 (fr) 1996-12-05

Family

ID=10772289

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/004347 WO1996030274A1 (fr) 1995-03-31 1996-03-29 Fermeture de tube

Country Status (2)

Country Link
GB (1) GB9506709D0 (fr)
WO (1) WO1996030274A1 (fr)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958778A (en) * 1995-09-22 1999-09-28 The United States Of America As Represented By The Department Of Health And Human Services Container for drying biological samples, method of making such container, and method of using same
US6312648B1 (en) 1998-01-12 2001-11-06 The United States Of America As Represented By The Department Of Health And Human Services Applicator system
EP1516920A1 (fr) * 2003-09-19 2005-03-23 The Automation Partnership Récipient de culture de cellules pour la culture automatisée de cultures de cellules
EP1963477A1 (fr) * 2005-12-22 2008-09-03 Sartorius Stedim Biotech GmbH Bioréacteur à usage unique pour la culture de cellules dans un milieu nutritif
WO2014207716A1 (fr) * 2013-06-27 2014-12-31 Genbiotech Utilisation d'un filtre pour decongeler des cellules
EP2917331A4 (fr) * 2012-11-09 2016-10-12 Biosystems Inc Aushon Procédés et systèmes pour la sensibilité de détection améliorée de dosages
US20160311674A1 (en) * 2013-12-06 2016-10-27 Allpure Technologies, Inc. Fluid transfer interface
WO2017082895A1 (fr) 2015-11-11 2017-05-18 Allpure Technologies, Llc Ensemble sensiblement aseptique pour traiter des fluides
WO2017209628A1 (fr) * 2016-05-31 2017-12-07 Crime Scene Solutions Limited Appareil de collecte et de stockage amélioré
US10006567B2 (en) 2011-06-22 2018-06-26 Sartorius Stedim North America, Inc. Vessel closures and methods for using and manufacturing same
US10486959B2 (en) 2011-06-22 2019-11-26 Sartorius Stedim North America Inc. Fluid transfer interface
US10773863B2 (en) 2011-06-22 2020-09-15 Sartorius Stedim North America Inc. Vessel closures and methods for using and manufacturing same
CN112410217A (zh) * 2020-11-06 2021-02-26 英诺维尔智能科技（苏州）有限公司 一种离心培养瓶
US11319201B2 (en) 2019-07-23 2022-05-03 Sartorius Stedim North America Inc. System for simultaneous filling of multiple containers
US11577953B2 (en) 2017-11-14 2023-02-14 Sartorius Stedim North America, Inc. System for simultaneous distribution of fluid to multiple vessels and method of using the same
US11691866B2 (en) 2017-11-14 2023-07-04 Sartorius Stedim North America Inc. System for simultaneous distribution of fluid to multiple vessels and method of using the same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4241188A (en) * 1979-10-09 1980-12-23 Becton, Dickinson And Company Culture bottle having stopper lock
JPS6398380A (ja) * 1986-10-15 1988-04-28 Santomi Sangyo Kk 組織培養方法及びこれに使用するキヤツプ
US5523236A (en) * 1994-08-18 1996-06-04 Becton, Dickinson And Company Closure assembly for cell culture vessels

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4241188A (en) * 1979-10-09 1980-12-23 Becton, Dickinson And Company Culture bottle having stopper lock
JPS6398380A (ja) * 1986-10-15 1988-04-28 Santomi Sangyo Kk 組織培養方法及びこれに使用するキヤツプ
US5523236A (en) * 1994-08-18 1996-06-04 Becton, Dickinson And Company Closure assembly for cell culture vessels

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6503455B1 (en) 1995-09-22 2003-01-07 The United States Of America As Represented By The Department Of Health And Human Services Container for dying biological samples, method of making such container, and method of using same
US5958778A (en) * 1995-09-22 1999-09-28 The United States Of America As Represented By The Department Of Health And Human Services Container for drying biological samples, method of making such container, and method of using same
US6312648B1 (en) 1998-01-12 2001-11-06 The United States Of America As Represented By The Department Of Health And Human Services Applicator system
EP1516920A1 (fr) * 2003-09-19 2005-03-23 The Automation Partnership Récipient de culture de cellules pour la culture automatisée de cultures de cellules
EP1963477A1 (fr) * 2005-12-22 2008-09-03 Sartorius Stedim Biotech GmbH Bioréacteur à usage unique pour la culture de cellules dans un milieu nutritif
US9174181B2 (en) 2005-12-22 2015-11-03 Sartorius Stedim Biotech Gmbh Disposable bioreactor for culturing cells in a nutrient medium
US10006567B2 (en) 2011-06-22 2018-06-26 Sartorius Stedim North America, Inc. Vessel closures and methods for using and manufacturing same
US11584571B2 (en) 2011-06-22 2023-02-21 Sartorius Stedim North America Inc. Vessel closures and methods for using and manufacturing same
US10773863B2 (en) 2011-06-22 2020-09-15 Sartorius Stedim North America Inc. Vessel closures and methods for using and manufacturing same
US10486959B2 (en) 2011-06-22 2019-11-26 Sartorius Stedim North America Inc. Fluid transfer interface
US10191037B2 (en) 2012-11-09 2019-01-29 Aushon Biosystems, Inc. Methods of and systems for improved detection sensitivity of assays
EP2917331A4 (fr) * 2012-11-09 2016-10-12 Biosystems Inc Aushon Procédés et systèmes pour la sensibilité de détection améliorée de dosages
US10100276B2 (en) 2013-06-27 2018-10-16 Genbiotech Use of a filter for defrosting cells
WO2014207716A1 (fr) * 2013-06-27 2014-12-31 Genbiotech Utilisation d'un filtre pour decongeler des cellules
US10647565B2 (en) 2013-12-06 2020-05-12 Sartorius Stedium North America, Inc. Fluid transfer interface
US20160311674A1 (en) * 2013-12-06 2016-10-27 Allpure Technologies, Inc. Fluid transfer interface
CN108778945B (zh) * 2015-11-11 2020-10-27 赛多利斯史泰迪北美股份有限公司 用于处理流体的基本无菌组件
WO2017082895A1 (fr) 2015-11-11 2017-05-18 Allpure Technologies, Llc Ensemble sensiblement aseptique pour traiter des fluides
US11103871B2 (en) 2015-11-11 2021-08-31 Sartorius Stedim North America Inc. Substantially aseptic assembly for processing fluids
CN108778945A (zh) * 2015-11-11 2018-11-09 赛多利斯史泰迪北美股份有限公司 用于处理流体的基本无菌组件
AU2017276141B2 (en) * 2016-05-31 2019-12-19 Crime Scene Solutions Limited Improved collection and storage apparatus
WO2017209628A1 (fr) * 2016-05-31 2017-12-07 Crime Scene Solutions Limited Appareil de collecte et de stockage amélioré
US11035758B2 (en) 2016-05-31 2021-06-15 Crime Scene Solutions Limited Collection and storage apparatus
US11577953B2 (en) 2017-11-14 2023-02-14 Sartorius Stedim North America, Inc. System for simultaneous distribution of fluid to multiple vessels and method of using the same
US11623856B2 (en) 2017-11-14 2023-04-11 Sartorius Stedim North America Inc. System for simultaneous distribution of fluid to multiple vessels and method of using the same
US11691866B2 (en) 2017-11-14 2023-07-04 Sartorius Stedim North America Inc. System for simultaneous distribution of fluid to multiple vessels and method of using the same
US11319201B2 (en) 2019-07-23 2022-05-03 Sartorius Stedim North America Inc. System for simultaneous filling of multiple containers
CN112410217A (zh) * 2020-11-06 2021-02-26 英诺维尔智能科技（苏州）有限公司 一种离心培养瓶

Also Published As

Publication number Publication date
GB9506709D0 (en) 1995-05-24

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