WO1996018634B1 - Derives de nucleosides comportant des groupes protecteurs photolabiles - Google Patents

Derives de nucleosides comportant des groupes protecteurs photolabiles

Info

Publication number
WO1996018634B1
WO1996018634B1 PCT/EP1995/004976 EP9504976W WO9618634B1 WO 1996018634 B1 WO1996018634 B1 WO 1996018634B1 EP 9504976 W EP9504976 W EP 9504976W WO 9618634 B1 WO9618634 B1 WO 9618634B1
Authority
WO
WIPO (PCT)
Prior art keywords
halogen
och
amino
guanine
cytosine
Prior art date
Application number
PCT/EP1995/004976
Other languages
German (de)
English (en)
Other versions
WO1996018634A2 (fr
WO1996018634A3 (fr
Filing date
Publication date
Priority claimed from DE4444996A external-priority patent/DE4444996A1/de
Priority to DE59510162T priority Critical patent/DE59510162D1/de
Priority to US08/693,217 priority patent/US5763599A/en
Priority to BR9510498-4A priority patent/BR9510498A/pt
Priority to CZ19971836A priority patent/CZ292296B6/cs
Priority to AU43865/96A priority patent/AU692658B2/en
Priority to AT95942675T priority patent/ATE215957T1/de
Priority to EP95942675A priority patent/EP0797580B1/fr
Application filed filed Critical
Priority to DK95942675T priority patent/DK0797580T3/da
Priority to HU9701821A priority patent/HU215543B/hu
Priority to JP8518276A priority patent/JPH11501287A/ja
Publication of WO1996018634A2 publication Critical patent/WO1996018634A2/fr
Publication of WO1996018634A3 publication Critical patent/WO1996018634A3/fr
Publication of WO1996018634B1 publication Critical patent/WO1996018634B1/fr
Priority to NO972754A priority patent/NO307382B1/no
Priority to FI973643A priority patent/FI973643A/fi

Links

Abstract

La présente invention concerne des dérivés de nucléosides comportant des groupes protecteurs photolabiles, de formule générale (I) où R1 = H, NO¿2?, CN, OCH3, halogène ou alcoyle ou alcoxyalcoyle ayant de 1 à 4 atomes de C; R?2¿ = H, OCH¿3; R?3 = H, F, Cl, Br, NO¿2; R?4 = H, halogène, OCH¿3? ou un reste d'alcoyle ayant de 1 à 4 atomes de C; R?5¿ = H ou un groupe protecteur fonctionnel usuel pour l'atome d'oxygène, convenant pour la préparation d'oligonucléotides; R6 = H, OH, halogène ou XR8, avec X = 0 ou S et R8 étant un groupe protecteur usuel en chimie des nucléotides; B = adénine, cytosine, guanine, thymine, uracile, 2, 6-diaminopurin-9-yle, hypoxanthin-9-yle, 5-méthylcytosin-1-yle, 5-amino-4-amide d'acide carboxylique imidazol-1-yle ou 5-amino-4-amide d'acide carboxylique imidazol-3-yle. Dans le cas où B = adénine, cytosine ou guanine, la fonction amino primaire comporte, le cas échéant, un groupe protecteur permanent. Ces dérivés peuvent être employés pour la synthèse, commandée par la lumière, d'oligonucléotides sur une plaquette d'ADN.
PCT/EP1995/004976 1994-12-16 1995-12-15 Derives de nucleosides comportant des groupes protecteurs photolabiles WO1996018634A2 (fr)

Priority Applications (12)

Application Number Priority Date Filing Date Title
JP8518276A JPH11501287A (ja) 1994-12-16 1995-12-15 光に不安定な保護基を有するヌクレオシド誘導体
EP95942675A EP0797580B1 (fr) 1994-12-16 1995-12-15 Derives de nucleosides comportant des groupes protecteurs photolabiles
BR9510498-4A BR9510498A (pt) 1994-12-16 1995-12-15 Derivados de nucleosìdeos com grupos de proteção fotolábeis
CZ19971836A CZ292296B6 (cs) 1994-12-16 1995-12-15 Deriváty nukleosidů s fotolabilními chránicími skupinami, způsob jejich přípravy a jejich použití
DK95942675T DK0797580T3 (da) 1994-12-16 1995-12-15 Nucleosidderivater med fotolabile beskyttelsesgrupper
AT95942675T ATE215957T1 (de) 1994-12-16 1995-12-15 Nucleosid-derivate mit photolabilen schutzgruppen
US08/693,217 US5763599A (en) 1994-12-16 1995-12-15 Nucleoside derivatives with photolabile protective groups
DE59510162T DE59510162D1 (de) 1994-12-16 1995-12-15 Nucleosid-derivate mit photolabilen schutzgruppen
AU43865/96A AU692658B2 (en) 1994-12-16 1995-12-15 Nucleoside derivatives with photolabile protective groups
HU9701821A HU215543B (hu) 1994-12-16 1995-12-15 Fényérzékeny védőcsoportot tartalmazó nukleozidszármazékok, eljárás előállításukra és alkalmazásuk
NO972754A NO307382B1 (no) 1994-12-16 1997-06-13 Nukleosidderivater med fotolabile beskyttende grupper
FI973643A FI973643A (fi) 1994-12-16 1997-09-09 Valonherkkiä suojausryhmiä sisältäviä nukleosidijohdoksia

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4444996.8 1994-12-16
DE4444996A DE4444996A1 (de) 1994-12-16 1994-12-16 Nucleosid-Derivate mit photolabilen Schutzgruppen

Publications (3)

Publication Number Publication Date
WO1996018634A2 WO1996018634A2 (fr) 1996-06-20
WO1996018634A3 WO1996018634A3 (fr) 1996-08-22
WO1996018634B1 true WO1996018634B1 (fr) 1996-09-26

Family

ID=6536071

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1995/004976 WO1996018634A2 (fr) 1994-12-16 1995-12-15 Derives de nucleosides comportant des groupes protecteurs photolabiles

Country Status (17)

Country Link
US (1) US5763599A (fr)
EP (1) EP0797580B1 (fr)
JP (1) JPH11501287A (fr)
KR (1) KR100395290B1 (fr)
AT (1) ATE215957T1 (fr)
AU (1) AU692658B2 (fr)
BR (1) BR9510498A (fr)
CA (1) CA2207912A1 (fr)
CZ (1) CZ292296B6 (fr)
DE (2) DE4444996A1 (fr)
DK (1) DK0797580T3 (fr)
ES (1) ES2174976T3 (fr)
FI (1) FI973643A (fr)
HU (1) HU215543B (fr)
IL (1) IL116407A (fr)
NO (1) NO307382B1 (fr)
WO (1) WO1996018634A2 (fr)

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US6147205A (en) * 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
WO1997041139A2 (fr) 1996-04-17 1997-11-06 Koester Hubert Strategie combinatoire par groupes de protection pour molecules multifonctionnelles
DE19620170A1 (de) * 1996-05-20 1997-11-27 Wolfgang Prof Dr Dr Pfleiderer Nucleosid-Derivate mit photolabilen Schutzgruppen
US7138384B1 (en) 1997-08-29 2006-11-21 The Regents Of The University Of California Modulators of DNA cytosine-5 methyltransferase and methods for use thereof
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WO2000011446A2 (fr) 1998-08-21 2000-03-02 University Of Virginia Patent Foundation Biocapteur generateur de signaux base sur les oligonucleotides
US6187540B1 (en) 1998-11-09 2001-02-13 Identigene, Inc. Method of newborn identification and tracking
DE19858440A1 (de) * 1998-12-17 2000-06-21 Deutsches Krebsforsch Verfahren zur photolithografischen Biochip-Synthese
EP1163251A1 (fr) 1999-03-24 2001-12-19 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES N-acylphosphoramidites et leur utilisation dans la synthese d'oligonucleotides
WO2000061594A2 (fr) * 1999-04-08 2000-10-19 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Derives de nucleosides dotes de groupes protecteurs photo-instables
DE19952113A1 (de) * 1999-10-29 2001-05-03 Nigu Chemie Gmbh Nucleosid-Derivate mit photolabilen Schutzgruppen
EP1176151B1 (fr) * 2000-07-28 2014-08-20 Agilent Technologies, Inc. Synthèse des polynucléotides utilisant la chimie combinée d'oxidation et de protection
CA2421732A1 (fr) * 2000-09-11 2002-03-14 Affymetrix, Inc. Groupes de protection photoclivables
DE10105077A1 (de) * 2001-02-05 2002-08-08 Febit Ferrarius Biotech Gmbh Hybrid-Schutzgruppe
DE10105079A1 (de) * 2001-02-05 2002-08-08 Febit Ferrarius Biotech Gmbh Fotolabile Schutzgruppen für die Synthese von Biopolymeren
EP1401851B8 (fr) * 2001-07-03 2008-09-10 febit holding GmbH Groupes protecteurs separes en deux etapes pour la synthese de biopolymeres
DE10152147A1 (de) * 2001-10-25 2003-05-15 Chemogenix Gmbh Verfahren zum Aufbringen von Nukleosiden und/oder Nukleotiden auf funktionalisierten Oberflächen sowie Verfahren zur Bestimmung von Kopplungsausbeuten bei der Synthese von Nukleotiden
DE10156467A1 (de) * 2001-11-16 2003-06-05 Micronas Gmbh Träger zur Synthese sowie Verfahren zur Herstellung von Biopolymeren
WO2003048179A2 (fr) * 2001-12-03 2003-06-12 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Groupes protecteurs hydroxyle thermolabiles et procedes permettant de les utiliser
EP1480927B1 (fr) 2002-03-04 2012-12-26 Universität Konstanz Procede de clivage de groupes fonctionnels labiles a partir de composes chimiques
DE10209203A1 (de) 2002-03-04 2003-09-25 Ulrich Steiner Verfahren zur Abspaltung von labilen funktionellen Gruppen aus chemischen Verbindungen
AU2003290117A1 (en) * 2002-12-23 2004-07-22 Febit Biotech Gmbh Photoactivatable two-stage protective groups for the synthesis of biopolymers
US20040157220A1 (en) 2003-02-10 2004-08-12 Purnima Kurnool Methods and apparatus for sample tracking
US7759513B2 (en) * 2003-02-21 2010-07-20 Nigu Chemie Gmbh Photolabile protective groups for improved processes to prepare oligonucleotide arrays
DE10315772A1 (de) * 2003-04-07 2004-11-04 Ulrich Prof. Dr. Steiner Chemische Verbindung und Verfahren für den intramolekularen Energietransfer zur Abspaltung von labilen funktionellen Gruppen aus chemischen Verbindungen
US7612197B2 (en) * 2003-05-09 2009-11-03 The United States of America as repesented by the Secretary of the Department of Health and Human Services Thermolabile hydroxyl protecting groups and methods of use
DE102004019098A1 (de) * 2004-04-20 2005-11-10 Chemogenix Gmbh Photolabile Schutzgruppen
US9809824B2 (en) * 2004-12-13 2017-11-07 The United States Of America, Represented By The Secretary, Department Of Health And Human Services CpG oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
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US8338093B2 (en) * 2004-12-31 2012-12-25 Affymetrix, Inc. Primer array synthesis and validation
US8343710B1 (en) 2005-03-11 2013-01-01 The Regents Of The University Of Colorado, A Body Corporate Photodegradable groups for tunable polymeric materials
US9180196B2 (en) 2005-03-11 2015-11-10 The Regents Of The University Of Colorado, A Body Corporate Photodegradable groups for tunable polymeric materials
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US9139874B2 (en) 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
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