WO1995020968A1 - Agent prophylactique pour la mastite des animaux domestiques - Google Patents

Agent prophylactique pour la mastite des animaux domestiques Download PDF

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Publication number
WO1995020968A1
WO1995020968A1 PCT/JP1994/000160 JP9400160W WO9520968A1 WO 1995020968 A1 WO1995020968 A1 WO 1995020968A1 JP 9400160 W JP9400160 W JP 9400160W WO 9520968 A1 WO9520968 A1 WO 9520968A1
Authority
WO
WIPO (PCT)
Prior art keywords
chitosan
mastitis
quaternary ammonium
fungicide
ammonium salt
Prior art date
Application number
PCT/JP1994/000160
Other languages
English (en)
Japanese (ja)
Inventor
Shigeru Saito
Megumi Tomita
Original Assignee
Nippon Soda Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP4233032A priority Critical patent/JPH0656675A/ja
Application filed by Nippon Soda Co., Ltd. filed Critical Nippon Soda Co., Ltd.
Priority to PCT/JP1994/000160 priority patent/WO1995020968A1/fr
Priority to EP94905842A priority patent/EP0692253A4/fr
Publication of WO1995020968A1 publication Critical patent/WO1995020968A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/722—Chitin, chitosan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

Definitions

  • the present invention relates to an agent for preventing mastitis used for preventing mastitis in livestock. More specifically, it is characterized by forming a film containing a quaternary ammonium salt fungicide having antibacterial and bactericidal properties and chitosan and / or a chitosan derivative on livestock such as dairy cows. 3 related to mastitis prevention agent
  • Dairy cows ⁇ Mastitis in any domestic animal is a disease in which Staphylococcus aureus, Streptococcus aga lactiae, Escherichia coli and other pathogens invade the udder through the nipple and cause inflammation.
  • the disease has a high incidence, reduced milk yield, reduced milk quality, reduced service life, and public health problems and somatic cells due to bacterial contamination of milk. It is a serious illness that causes large losses, such as poor yields when processing dairy products due to an increase in dairy products.
  • mastitis is a serious disease of livestock.However, in the past, appropriate measures for raising livestock to increase resistance to pathogens and clean milking have been used, as well as for measures such as povidone land, hordehole, and glucoside. Preventive methods such as disinfecting (dipping) the nipple with a teat dip containing a bactericide such as chlorhexidine acid chloride and benzalkonium chloride are known.
  • nipple pacifiers that form a film that blocks the nipple from pathogens in the external environment are also used, but are not themselves bactericidal and cannot prevent the transmission of pathogens during milking. :
  • teat dips is effective in reducing the incidence of mastitis, but the effect is It depends on the disinfecting power, disinfecting speed, disinfecting bacteria type, and persistence of the disinfecting agent. In addition to the sterilizing effect, it is important not to cause damage to the skin and mucous membranes of dairy cows and workers.
  • Nipple immersion containing a fungicide such as poppy donde is widely used, but it has high irritation to the skin and mucous membranes. cause.
  • Cucorhexidine dalconate fungicides have problems of low sterilization rate and mucosal irritation.
  • Benzalkonim dichloride fungicide has the advantage of being less irritating to the skin, but some bactericidal bacteria have insufficient bactericidal activity. Further, these disinfectants do not have a film property and are easily washed away by water, so that there is a problem that the disinfecting power is not maintained for a long time.
  • the present invention provides a new agent for preventing mastitis that forms a coating on the nipples of livestock animals such as dairy cows, suppresses the growth of pathogenic bacteria in the nipples, prevents the invasion of pathogenic bacteria into the udder, and has little skin irritation
  • the task is to
  • a mastitis preventive agent comprising a combination of chitosan or a chitosan derivative and a quaternary ammonium salt fungicide has a strong bactericidal activity.
  • the present inventors have found that the invasion of the pathogenic bacteria into the breast can be prevented by forming a good film with little irritation to the skin and completing the present invention.
  • the agent for preventing mastitis according to the present invention is characterized by comprising a quaternary ammonium salt-based bactericide and chitosan and Z or a chitosan derivative as active ingredients.
  • chitosan or chitosan derivatives have various functions such as antibacterial property, bactericidal property, wound healing action, immunostimulatory action, etc., and form a highly biocompatible film. It is known for use in the fields of industry, agriculture and food.
  • the present inventors have first focused on the excellent characteristics of the above-described chitosan or chitosan derivative, and have proposed an aqueous solution of chitosan or chitosan derivative for preventing mastitis in livestock such as dairy cows.
  • the chitosan used in the present invention is obtained by deacetylating chitin (a polysaccharide of X-acetyl-D-glucosamine), which is a constituent of shells of crustaceans such as Rikido and insects such as beetles. It is a basic poly-macromolecular substance having a free amino group, and the molecular weight is not particularly limited.
  • the chitosan derivative used in the present invention also includes chitin, which is a raw material that has not been deacetylated, and a substance containing a mildly decomposed product generated by a chemical treatment.
  • All or part of the free amino group or hydroxyl group is an alkyl group, an alkoxyalkyl group, an alkylthioalkyl group, an aryl group, an aralkyl group, an acyl group, a sulfonyl group, an alkoxycarbonyl group or a phosphoric acid residue And the like, which have been chemically modified.
  • chitosan derivatives can be obtained, for example, by a common synthetic chemical modification such as the method described in the following literature.
  • the mastitis preventive agent comprising the quaternary ammonium salt fungicide of the present invention and chitosan and Z or a chitosan derivative is quaternary ammonium based on 100 parts by weight of chitosan and Z or chitosan derivative. It contains the salt-based fungicide in an amount of 0.2 to 100 parts by weight, preferably 2 to 50 parts by weight.
  • the agent for preventing mastitis comprises an aqueous solution or an organic acid or a quaternary ammonium salt-based fungicide, preferably containing chitosan and a Z or chitosan derivative, preferably in an amount of 0.05 to 10% by weight. Used in dilute aqueous solutions of inorganic acids.
  • Examples of the acid used in the present invention include inorganic acids such as hydrochloric acid and nitric acid, and organic acids such as acetic acid, kunnic acid, succinic acid, lactic acid, and lingic acid.
  • the amount of the mastitis preventive agent used is not limited because it varies depending on the treatment method and the concentration used, but it is appropriately selected and used within a range where the effect can be exhibited.
  • quaternary Anmoniumu salt fungicides for use in the present invention chloride Benzarukoni ⁇ , 3 ⁇ 4 of benzene Bok Niu ⁇ (C 2: H, 2 C 1 X 0 2), cetyl Application Benefits main Chiruanmo Niumuku b And didecyldimethylammonium chloride.
  • Representative compounds of the present invention include octyldimethylbenzylammonium chloride, decyldimethylbenzylammonium chloride, dodecyldimethylbenzylbenzylammonium chloride, tetradecyldimethylmethylbenzylammonium chloride, Hexadecyl dimethyl benzyl ammonium chloride, octadecyl dimethyl benzyl ammonium chloride, cocoalkyl dimethyl benzyl ammonium chloride and the like are exemplified.
  • the prepared quaternary ammonium salt fungicide and the mastitis preventive agent containing chitosan and / or a chitosan derivative can be applied to a film having strong bactericidal activity on the nipple by a method such as spraying or dipping. Mastitis can be prevented by forming pathogens, killing pathogens in the nipple, and preventing the pathogens from entering the udder.
  • Test bacteria Staphylococcus aureus (St hyi occus aureus I FO 12732;
  • Benzalconium chloride BAC Nissan cation M 2 -100 Nippon Oil & Fat
  • Benzetonium chloride BEC, Hyamin Sankyo
  • Alkyl glycine AG, Nissan Anon LG Nippon Oil Co., Ltd.
  • the values of MIC in Table 1 indicate the concentration of the fungicide excluding chitosan.
  • Cloth 1 (ug / m £ Fungicide name Staphylococcus aureus Lactococcus aureus Pseudomonas aeruginosa
  • test drugs of Examples 1 and 2 and Comparative Examples 1 to 4 having the components shown in Table 1 were prepared.
  • test drugs of Examples 1 and 2 and Comparative Examples 1 to 4 patch tests using a lint cloth were performed on the skin of 20 adult men. Pasting time is 6 hours. Twenty-four hours after the end of application, the condition of the skin was observed.
  • Table 3 summarizes the results of observation of the condition of nipple skin roughness during the mastitis prevention test.
  • Example 1 Example 2
  • Example 2 Ratio K Example 1 Component weight (g) Component weight (g) 'Component weight (g) Purified water 100 Purified water 100; Purified water 100 Glacial acetic acid 0.5? Citric acid 1.5, glacial acetic acid 0.5 Chitosan 1.0 ⁇ "7" no 1.0: Chitosan 1.0
  • Component weight (g) Component weight (g)! Component Weight (g) Purified water 100 Purified water 100! Purified water 100 Glacial acetic acid 0.5
  • Comparative example 3 35 '5.1% Cracking: Comparative example 4 30; 10 33.33 ⁇ 4; Good: Table
  • Test Example 1 when a quaternary ammonium salt-based bactericide is used in combination with chitosan and Z or a chitosan derivative, the bactericidal activity is not impaired, and a synergistic effect on the bactericidal activity is exhibited. There is also. The combination of another fungicide with chitosan did not give favorable results because the antibacterial activity was impaired, and the formic fungicide and chitosan were not compatible and were unsuitable for mixed use.
  • the mastitis prevention agent of the present invention showed an excellent effect as compared with other comparative test drugs.
  • the flame preventive agent caused cracks in the nipple due to skin irritation.
  • the mastitis preventive agent of the present invention exhibits excellent bactericidal activity by combining a specific bactericide with chitosan and Z or a chitosan derivative, and has a problem of cracking the nipple, which has been a problem in the past. It is characterized by having no skin irritation such as causing a cause and preventing the invasion of pathogenic bacteria into the breast by good film formation of chitosan and / or a chitosan derivative.
  • the present invention provides a novel anti-mastitis agent that forms a film on the nipple of livestock animals such as dairy cows, suppresses the growth of pathogenic bacteria in the nipple, and prevents the invasion of pathogenic bacteria into the udder. To provide farmers with an effective means of preventing livestock mastitis.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un agent prophylactique comprenant une combinaison de chitosane ou d'un dérivé de ce dernier et d'un bactéricide au sel d'ammonium quaternaire. Cet agent présente une activité bactéricide puissante, et peut former une pellicule de chitosane de bonne qualité pour prévenir efficacement la mastite. Cet agent irritant modérément la peau, il est moins susceptible que d'autres médicaments d'induire la formation de crevasses du mamelon d'origine médicamenteuse.
PCT/JP1994/000160 1992-08-07 1994-02-03 Agent prophylactique pour la mastite des animaux domestiques WO1995020968A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP4233032A JPH0656675A (ja) 1992-08-07 1992-08-07 家畜の乳房炎予防剤
PCT/JP1994/000160 WO1995020968A1 (fr) 1992-08-07 1994-02-03 Agent prophylactique pour la mastite des animaux domestiques
EP94905842A EP0692253A4 (fr) 1992-08-07 1994-02-03 Agent prophylactique pour la mastite des animaux domestiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP4233032A JPH0656675A (ja) 1992-08-07 1992-08-07 家畜の乳房炎予防剤
PCT/JP1994/000160 WO1995020968A1 (fr) 1992-08-07 1994-02-03 Agent prophylactique pour la mastite des animaux domestiques

Publications (1)

Publication Number Publication Date
WO1995020968A1 true WO1995020968A1 (fr) 1995-08-10

Family

ID=26435268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1994/000160 WO1995020968A1 (fr) 1992-08-07 1994-02-03 Agent prophylactique pour la mastite des animaux domestiques

Country Status (2)

Country Link
JP (1) JPH0656675A (fr)
WO (1) WO1995020968A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0656675A (ja) * 1992-08-07 1994-03-01 Nippon Soda Co Ltd 家畜の乳房炎予防剤
US6054462A (en) * 1994-04-13 2000-04-25 Janssen Pharmaceutica, N.V. Intranasal antimigraine compositions
HRP950149A2 (en) * 1994-04-13 1997-06-30 Janssen Pharmaceutica Nv Intranasal antimigrene composition
KR100299669B1 (ko) * 1998-05-02 2001-10-27 민경우 젖소의 유두 소독과 유방염 예방을 위한 유두침지액
KR100321932B1 (ko) * 1998-08-18 2002-09-05 세원화성 주식회사 키토산 또는 키토산 올리고당을유효성분으로 하는 젖소의 유방염 치료제
JP2000329447A (ja) 1999-05-17 2000-11-30 Matsushita Refrig Co Ltd 冷蔵庫および除霜用ヒーター
KR100671847B1 (ko) * 2005-04-16 2007-01-19 한국스테비아(주) 착유우의 체세포 감소제
RU2467755C1 (ru) * 2011-09-08 2012-11-27 Государственное научное учреждение Всероссийский научно-исследовательский ветеринарный институт патологии, фармакологии и терапии Российской академии сельскохозяйственных наук (ГНУ ВНИВИПФиТ Россельхозакадемии) Способ комбинированного лечения послеродового эндометрита у коров
WO2015072125A1 (fr) * 2013-11-14 2015-05-21 日本曹達株式会社 Composition anti-parvovirus

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5946223A (ja) * 1982-08-30 1984-03-15 Daito Koeki Kk 抗菌性組成物
JPH04169529A (ja) * 1990-10-30 1992-06-17 Nippon Soda Co Ltd 家蓄の乳房炎を予防する方法
JPH04178329A (ja) * 1990-11-09 1992-06-25 Unitika Ltd 抗菌性材料及び抗菌性創傷被覆保護材
JPH0656675A (ja) * 1992-08-07 1994-03-01 Nippon Soda Co Ltd 家畜の乳房炎予防剤

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5946223A (ja) * 1982-08-30 1984-03-15 Daito Koeki Kk 抗菌性組成物
JPH04169529A (ja) * 1990-10-30 1992-06-17 Nippon Soda Co Ltd 家蓄の乳房炎を予防する方法
JPH04178329A (ja) * 1990-11-09 1992-06-25 Unitika Ltd 抗菌性材料及び抗菌性創傷被覆保護材
JPH0656675A (ja) * 1992-08-07 1994-03-01 Nippon Soda Co Ltd 家畜の乳房炎予防剤

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0692253A4 *

Also Published As

Publication number Publication date
JPH0656675A (ja) 1994-03-01

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