WO1995017661A1 - Device for raising the liquid level in a well for measuring the density - Google Patents

Device for raising the liquid level in a well for measuring the density Download PDF

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Publication number
WO1995017661A1
WO1995017661A1 PCT/FR1994/001467 FR9401467W WO9517661A1 WO 1995017661 A1 WO1995017661 A1 WO 1995017661A1 FR 9401467 W FR9401467 W FR 9401467W WO 9517661 A1 WO9517661 A1 WO 9517661A1
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WO
WIPO (PCT)
Prior art keywords
well
plate
sleeves
substance
raising
Prior art date
Application number
PCT/FR1994/001467
Other languages
French (fr)
Inventor
Marc Dumas
Frédéric Bonte
Alain Meybeck
Original Assignee
Lvmh Recherche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lvmh Recherche filed Critical Lvmh Recherche
Priority to JP7517222A priority Critical patent/JPH08510558A/en
Priority to EP95904560A priority patent/EP0685063A1/en
Priority to AU13192/95A priority patent/AU1319295A/en
Publication of WO1995017661A1 publication Critical patent/WO1995017661A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment

Definitions

  • the present invention essentially relates to a device for raising the level of a liquid, in a cell, well or the like.
  • Cell or multiple well plates frequently intended to contain one or more substances to be analyzed are frequently used in biology, in particular in cell biology.
  • - ⁇ is the molar extinction coefficient, the value of which depends on the wavelength of the light used and the nature of the dissolved substance.
  • C is the concentration of the substance in the solution
  • 1 0 is the length of the optical path in the solution sample.
  • the object of the present invention is to solve the above problems. To this end, it relates to a device for raising the level of a liquid containing a dissolved substance, in a cell, well or the like, characterized by a wall sleeve of variable thickness and open at its two ends, which is insertable into said cell or well.
  • the sleeve is integral with an element comprising a plurality of such sleeves.
  • This element can be a bar or a plate.
  • It can include notching lines between the sleeves to allow their separation so as to use only part of the set of sleeves.
  • the invention also relates to a plate with cells or multiple wells allowing for example the culture of cells or the microtitration of samples, characterized in that it is provided with a device corresponding to the preceding characteristics.
  • This plate can be covered by a plate comprising 'on one side as many sleeves as wells in the plate.
  • the invention also relates to the use of the above device or plate for measuring the optical density of a substance dissolved in this liquid.
  • this device relates more particularly to the use of this device for the dosing of substances contained in a cell culture medium, these substances having for example been produced by cells in culture, or else having been released into the medium after solubilization of cells, these possibly having received an immunological labeling.
  • Figure 1 is a perspective view of a sleeve inserted into a well.
  • Figure 2 is a perspective view of a bar provided with such sleeves.
  • Figure 3 is a perspective view similar to Figure 2 but showing individually separable sleeves of the bar.
  • Figure 4 is a sectional view of the bar of Figure 2, passing through the axis of the sleeves, and illustrated in the mounted position on a row of wells.
  • Figure 5 is a partial perspective view of a multiwell plate ready to receive a plate having on one side of the sleeves 1 according to the invention.
  • a device according to the invention is, in general, constituted by a sleeve 1 which can be inserted into a cell or well 2 formed in an element 3 and which may contain a liquid in which any substance is dissolved.
  • the sleeve 1 is open at its two ends to allow the passage of the light beam, and its wall 4 can have a variable thickness, which characterizes the caliber of the sleeve 1.
  • the sleeve 1 once introduced into the well 2, will reduce the volume of this well as a function of the thickness of the wall 4 and will therefore allow the liquid to rise in the well 2 of the desired height depending on the size used.
  • the sleeve 1, as well as the element 3 may be of any material such as for example a transparent plastic material for single use or reusable from one measurement to another after cleaning.
  • a light beam passing through the well and the liquid it contains preferably in a direction substantially parallel to the axis of the well which is generally substantially cylindrical, will allow a measurement of the optical density of the substance dissolved in the liquid whose the height in the well will be large enough to provide the desired sensitivity, accuracy and efficiency of the measurement.
  • the sleeve 1 may be integral with a support 5 having a plurality of such sleeves projecting from one side of said support.
  • the support 5 can consist of a bar carrying a plurality of sleeves 1, the spacing of which corresponds to the spacing between the wells 2 of a line of wells made in the element 3.
  • the bar-shaped support 5 can include notching lines 6 between the sleeves 1, which makes it easy to separate them from one another so as to insert them into such and such a well formed in the element 3.
  • FIG. 4 shows a linear set of sleeves 1 belonging to a bar 5, as shown in FIG. 2, which line of sleeves is inserted into the wells 2 with corresponding spacing, formed in the element 3.
  • the sleeves 1 can also be secured to a support in the form of a plate or plate 7, as can be seen in FIG. 5 where said sleeves 1 project from the same face of the plate 7.
  • This plate 7 with associated sleeves may comprise as many sleeves 1 as there are cells or wells 2, provided in a plate P with multiple wells allowing, for example, cell culture, microtitration of samples, assay operations or even other uses.
  • sleeves 1 can be used in only certain wells 2 of plate P, for example using a bar 5 with breakable or non-breaking sleeves, as seen in FIGS. 2 and 3 .
  • the material and the wall thickness of the sleeves 1 can be any, as well as the geometrical position of the sleeves on a plate, a bar or any other support.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a device for raising the level of a liquid containing a dissolved substance in a well or the like, as well as to a multiple well plate provided with such device. The device is characterized by a plurality of sleeves (1) with a variable thickness wall (4), said sleeves being opened at both ends and forming part of a support (7), and being insertable into the wells (2) of a plate (P). The device provides for a rise of the level of a liquid in a well in order to measure for example the optical density and may be used particularly to measure the optical density of a substance dissolved in a cell culture medium.

Description

DISPOSITIF D'ELEVATION DU NIVEAU D'UN LIQUIDE DANS UN ALVEOLE POUR LA MESURE DE LA DENSITE.DEVICE FOR RAISING THE LEVEL OF A LIQUID IN A CELL FOR MEASURING DENSITY.
La présente invention a essentiellement pour objet un dispositif d'élévation du niveau d'un liquide, dans un alvéole, puits ou analogue.The present invention essentially relates to a device for raising the level of a liquid, in a cell, well or the like.
5 Elle vise également une plaque à puits multiples pour la culture de cellules, la microtitration ou autre usage, équipée de ce dispositif.5 It also relates to a multi-well plate for cell culture, microtitration or other use, equipped with this device.
On utilise fréquemment en biologie, en particulier en 0 biologie cellulaire, des plaques à alvéoles ou puits multiples destinés à contenir une ou des substances à analyser.Cell or multiple well plates frequently intended to contain one or more substances to be analyzed are frequently used in biology, in particular in cell biology.
Mais lorsqu'on veut mesurer la densité optique d'une 5 substance en solution dans un liquide et contenue dans un puits, par exemple selon la méthode décrite par F. BURMEISTER et al., dans "Preprints of the XlVth IFSCC Congress, Barcelone, 1986, vol. I, il faut que la hauteur du liquide soit assez grande pour permettre une 0 mesure de densité optique qui soit suffisamment précise, et donc fiable.But when it is desired to measure the optical density of a substance in solution in a liquid and contained in a well, for example according to the method described by F. BURMEISTER et al., In "Preprints of the XlVth IFSCC Congress, Barcelona, 1986, vol.I, the height of the liquid must be large enough to allow a measurement of optical density which is sufficiently precise and therefore reliable.
En effet, la densité optique qui se mesure en éclairant un échantillon transparent ou translucide par un 5 faisceau lumineux généralement monochromatique, est fonction de la longueur du trajet optique du faisceau lumineux, de la concentration de la substance dans la solution à mesurer et du coefficient d'absorption de la lumière propre à la substance, comme établi par la 0 formule suivante : D0 = ε c l0 dans laquelle :Indeed, the optical density which is measured by illuminating a transparent or translucent sample with a generally monochromatic light beam, is a function of the length of the optical path of the light beam, of the concentration of the substance in the solution to be measured and of the coefficient substance-specific light absorption, as established by the following formula 0: D 0 = ε cl 0 in which:
- D0 est la densité optique.- D 0 is the optical density.
- ε est le coefficient d'extinction molaire, dont la valeur dépend de la longueur d'onde de la lumière utilisée et de la nature de la substance dissoute.- ε is the molar extinction coefficient, the value of which depends on the wavelength of the light used and the nature of the dissolved substance.
C est la concentration de la substance dans la solution, etC is the concentration of the substance in the solution, and
10 est la longueur du trajet optique dans l'échantillon de solution.1 0 is the length of the optical path in the solution sample.
Or, bien souvent, on se trouve en face d'échantillons de très faible volume et dont la hauteur dans le puits et par conséquent le trajet optique, est très petite, ce qui constribue à une imprécision notable de la mesure de densité optique. Cette imprécision devient encore beaucoup plus importante, donc beaucoup plus gênante dans le cas où la valeur du coefficient d'extinction molaire de l'échantillon analysé est faible. Par ailleurs, l'augmentation de la hauteur de l'échantillon dans le puits par apport de liquide supplémentaire, ne constitue pas une solution au problème de l'amélioration de la précision des mesures, car cet apport a pour effet de diminuer la concentration de la substance du fait de la dilution réalisée.However, very often, we are faced with samples of very small volume and whose height in the well and consequently the optical path, is very small, which contributes to a notable imprecision of the optical density measurement. This imprecision becomes still much greater, therefore much more troublesome in the case where the value of the molar extinction coefficient of the sample analyzed is low. Furthermore, increasing the height of the sample in the well by adding additional liquid does not constitute a solution to the problem of improving the accuracy of the measurements, since this contribution has the effect of reducing the concentration of the substance due to the dilution achieved.
La présente invention a pour objet de résoudre les problèmes ci-dessus. A cet effet, elle a pour objet un dispositif d'élévation du niveau d'un liquide contenant une substance dissoute, dans un alvéole, puits ou analogue, caractérisé par un manchon à paroi d'épaisseur variable et ouvert à ses deux extrémités, qui est insérable dans ledit alvéole ou puits.The object of the present invention is to solve the above problems. To this end, it relates to a device for raising the level of a liquid containing a dissolved substance, in a cell, well or the like, characterized by a wall sleeve of variable thickness and open at its two ends, which is insertable into said cell or well.
Suivant une autre caractéristique de ce dispositif, le manchon est solidaire d'un élément comportant une pluralité de tels manchons.According to another characteristic of this device, the sleeve is integral with an element comprising a plurality of such sleeves.
Cet élément peut être une barrette ou une platine.This element can be a bar or a plate.
Il peut comporter des lignes d'entaillage entre les manchons pour permettre leur séparation de façon à n'utiliser qu'une partie de l'ensemble des manchons.It can include notching lines between the sleeves to allow their separation so as to use only part of the set of sleeves.
L'invention vise encore une plaque à alvéoles ou puits multiples permettant par exemple la culture de cellules ou la microtitration d'échantillons, caractérisée en ce qu'elle est munie d'un dispositif répondant aux caractéristiques précédentes.The invention also relates to a plate with cells or multiple wells allowing for example the culture of cells or the microtitration of samples, characterized in that it is provided with a device corresponding to the preceding characteristics.
Cette plaque peut être recouverte par une platine comportant' sur une face autant de manchons que de puits dans la plaque.This plate can be covered by a plate comprising 'on one side as many sleeves as wells in the plate.
L'invention vise également l'utilisation du dispositif ou de la plaque ci-dessus pour la mesure de la densité optique d'une substance dissoute dans ce liquide.The invention also relates to the use of the above device or plate for measuring the optical density of a substance dissolved in this liquid.
Elle vise plus particulièrement l'utilisation de ce dispositif pour le dosage de substances contenues dans un milieu de culture cellulaire, ces substances ayant été par exemple produites par des cellules en culture, ou bien ayant été libérées dans le milieu après solubilisation de cellules, celles-ci ayant éventuellement reçu un marquage immunologique.It relates more particularly to the use of this device for the dosing of substances contained in a cell culture medium, these substances having for example been produced by cells in culture, or else having been released into the medium after solubilization of cells, these possibly having received an immunological labeling.
Mais d'autres caractéristiques et avantages de l'invention apparaîtront mieux dans la description détaillée qui suit et se réfère aux dessins annexés, donnés uniquement à titre d'exemple, et dans lesquels.However, other characteristics and advantages of the invention will appear better in the detailed description which follows and which refers to the appended drawings, given solely by way of example, and in which.
La figure 1 est une vue en perspective d'un manchon inséré dans un puits.Figure 1 is a perspective view of a sleeve inserted into a well.
La figure 2 est une vue en perspective d'une barrette munie de tels manchons .Figure 2 is a perspective view of a bar provided with such sleeves.
La figure 3 est une vue en perspective similaire à la figure 2 mais montrant des manchons individuellement séparables de la barrette.Figure 3 is a perspective view similar to Figure 2 but showing individually separable sleeves of the bar.
La figure 4 est une vue en coupe de la barrette de la figure 2, passant par l'axe des manchons, et illustrée en position montée sur une rangée de puits.Figure 4 is a sectional view of the bar of Figure 2, passing through the axis of the sleeves, and illustrated in the mounted position on a row of wells.
La figure 5 est une vue en perspective partielle d'une plaque à puits multiples prête à recevoir une platine comportant sur une face des manchons suivant 1 ' invention.Figure 5 is a partial perspective view of a multiwell plate ready to receive a plate having on one side of the sleeves 1 according to the invention.
En se reportant tout particulièrement à la figure 1, on voit qu'un dispositif selon l'invention est, d'une manière générale, constitué par un manchon 1 insérable dans un alvéole ou puits 2 ménagé dans un élément 3 et pouvant contenir un liquide dans lequel est dissoute une substance quelconque.With particular reference to FIG. 1, it can be seen that a device according to the invention is, in general, constituted by a sleeve 1 which can be inserted into a cell or well 2 formed in an element 3 and which may contain a liquid in which any substance is dissolved.
Le manchon 1 est ouvert à ses deux extrémités pour permettre le passage du faisceau lumineux, et sa paroi 4 peut avoir une épaisseur variable, ce qui caractérise le calibre du manchon 1.The sleeve 1 is open at its two ends to allow the passage of the light beam, and its wall 4 can have a variable thickness, which characterizes the caliber of the sleeve 1.
Ainsi, comme on le comprend aisément, le manchon 1, une fois introduit dans le puits 2, réduira le volume de ce puits en fonction de l'épaisseur de la paroi 4 et permettra par conséquent la remontée du liquide dans le puits 2 de la hauteur désirée en fonction du calibre utilisé.Thus, as is easily understood, the sleeve 1, once introduced into the well 2, will reduce the volume of this well as a function of the thickness of the wall 4 and will therefore allow the liquid to rise in the well 2 of the desired height depending on the size used.
Le manchon 1, de même que l'élément 3 peuvent être en une matière quelconque telle que par exemple une matière plastique transparente à usage unique ou réutilisable d'une mesure à une autre après nettoyage.The sleeve 1, as well as the element 3 may be of any material such as for example a transparent plastic material for single use or reusable from one measurement to another after cleaning.
Ainsi, un faisceau lumineux traversant le puits et le liquide qu'il contient, de préférence selon une direction sensiblement parallèle à l'axe du puits qui est généralement sensiblement cylindrique, permettra une mesure de la densité optique de la substance dissoute dans le liquide dont la hauteur dans le puits sera suffisamment importante pour procurer la sensibilité, la précision et l'efficacité voulues de la mesure.Thus, a light beam passing through the well and the liquid it contains, preferably in a direction substantially parallel to the axis of the well which is generally substantially cylindrical, will allow a measurement of the optical density of the substance dissolved in the liquid whose the height in the well will be large enough to provide the desired sensitivity, accuracy and efficiency of the measurement.
Comme on le voit sur les figures 2 et 3, le manchon 1 peut être solidaire d'un support 5 comportant une pluralité de tels manchons faisant saillie d'un côté dudit support .As seen in Figures 2 and 3, the sleeve 1 may be integral with a support 5 having a plurality of such sleeves projecting from one side of said support.
Comme on le voit sur la figure 2, le support 5 peut être constitué par une barrette portant une pluralité de manchons 1 dont 1 ' espacement correspondra à l'espacement entre les puits 2 d'une ligne de puits ménagée dans l'élément 3. Le support en forme de barrette 5 peut comporter des lignes d'entaillage 6 entre les manchons 1, ce qui permet aisément de les séparer les uns des autres pour les insérer dans tel ou tel puits ménagé dans 1 ' élément 3.As can be seen in FIG. 2, the support 5 can consist of a bar carrying a plurality of sleeves 1, the spacing of which corresponds to the spacing between the wells 2 of a line of wells made in the element 3. The bar-shaped support 5 can include notching lines 6 between the sleeves 1, which makes it easy to separate them from one another so as to insert them into such and such a well formed in the element 3.
On a montré sur la figure 4, un ensemble linéaire de manchons 1 appartenant à une barrette 5, comme montré sur la figure 2, laquelle ligne de manchons est insérée dans les puits 2 d'espacement correspondant, ménagés dans 1 'élément 3.FIG. 4 shows a linear set of sleeves 1 belonging to a bar 5, as shown in FIG. 2, which line of sleeves is inserted into the wells 2 with corresponding spacing, formed in the element 3.
Les manchons 1 peuvent aussi être solidaires d'un support en forme de plaque ou de platine 7, comme on le voit bien sur la figure 5 où lesdits manchons 1 font saillie d'une même face de la platine 7.The sleeves 1 can also be secured to a support in the form of a plate or plate 7, as can be seen in FIG. 5 where said sleeves 1 project from the same face of the plate 7.
Cette platine 7 avec manchons associés pourra comporter autant de manchons 1 que d'alvéoles ou puits 2, prévus dans une plaque P à puits multiples permettant par exemple la culture de cellules, la microtitration d'échantillons, des opérations de dosage ou encore d'autres utilisations.This plate 7 with associated sleeves may comprise as many sleeves 1 as there are cells or wells 2, provided in a plate P with multiple wells allowing, for example, cell culture, microtitration of samples, assay operations or even other uses.
II va de soi que l'on peut utiliser des manchons 1 dans seulement certains puits 2 de la plaque P, par exemple à l'aide d'une barrette 5 avec manchons sécables ou insécables, comme on le voit sur les figures 2 et 3.It goes without saying that sleeves 1 can be used in only certain wells 2 of plate P, for example using a bar 5 with breakable or non-breaking sleeves, as seen in FIGS. 2 and 3 .
Bien entendu, l'invention n'est nullement limitée au mode de réalisation décrit et illustré qui n'a été donné qu'à titre d'exemple.Of course, the invention is in no way limited to the embodiment described and illustrated, which has been given only by way of example.
C'est ainsi que la matière et l'épaisseur de paroi des manchons 1 peuvent être quelconques, de même que la position géométrique des manchons sur une platine, une barrette ou autre support quelconque.This is how the material and the wall thickness of the sleeves 1 can be any, as well as the geometrical position of the sleeves on a plate, a bar or any other support.
C'est dire que l'invention comprend tous les équivalents techniques des moyens décrits ainsi que leurs combinaisons si celles-ci sont effectuées suivant son esprit. This means that the invention includes all the technical equivalents of the means described and their combinations if these are carried out according to the spirit.

Claims

Revendications claims
1. Dispositif d'élévation du niveau d'un liquide contenant une substance dissoute, dans un alvéole, puits ou analogue (2) , caractérisé par un manchon (1) à paroi (4) d'épaisseur variable et ouvert à ses deux extrémités, qui est insérable dans ledit alvéole ou puits (2) .1. Device for raising the level of a liquid containing a dissolved substance, in a cell, well or the like (2), characterized by a sleeve (1) with a wall (4) of variable thickness and open at its two ends , which can be inserted into said cell or well (2).
2. Dispositif selon la revendication 1, caractérisé en ce que le manchon (1) est solidaire d'un support comportant une pluralité de tels manchons (1) .2. Device according to claim 1, characterized in that the sleeve (1) is integral with a support comprising a plurality of such sleeves (1).
3. Dispositif selon la revendication 2, caractérisé en ce que le support précité est une barrette (5) ou une platine (7) .3. Device according to claim 2, characterized in that the aforementioned support is a bar (5) or a plate (7).
4. Dispositif selon l'une des revendications précédentes, caractérisé en que le support précité comporte des lignes d'entaillage (6) entre les manchons (1) pour permettre leur séparation.4. Device according to one of the preceding claims, characterized in that the aforementioned support comprises notching lines (6) between the sleeves (1) to allow their separation.
5. Plaque à alvéoles ou puits multiples (2) permettant par exemple la culture de cellules ou la microtitration d'échantillons, caractérisé en ce qu'elle est munie d'un dispositif selon l'une des revendications 1 à 4.5. Plate with cells or multiple wells (2) allowing for example the culture of cells or the microtitration of samples, characterized in that it is provided with a device according to one of claims 1 to 4.
6. Plaque selon la revendication 5, caractérisée en ce qu'elle est recouverte par une platine (7) comportant sur une face autant de manchons (1) que de puits (2) dans la plaque (P) .6. Plate according to claim 5, characterized in that it is covered by a plate (7) comprising on one face as many sleeves (1) as wells (2) in the plate (P).
7. Utilisation du dispositif ou de la plaque selon l'une des revendications 1 à 6 pour la mesure de la densité optique d'une substance dissoute dans ce liquide. 7. Use of the device or plate according to one of claims 1 to 6 for measuring the optical density of a substance dissolved in this liquid.
8. Utilisation selon la revendication 7, pour le dosage de substances contenues dans un milieu de culture cellulaire . 8. Use according to claim 7, for the determination of substances contained in a cell culture medium.
PCT/FR1994/001467 1993-12-20 1994-12-14 Device for raising the liquid level in a well for measuring the density WO1995017661A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP7517222A JPH08510558A (en) 1993-12-20 1994-12-14 Device for raising liquid level in recesses for density measurement
EP95904560A EP0685063A1 (en) 1993-12-20 1994-12-14 Device for raising the liquid level in a well for measuring the density
AU13192/95A AU1319295A (en) 1993-12-20 1994-12-14 Device for raising the liquid level in a well for measuring the density

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR93/15314 1993-12-20
FR9315314A FR2714183B1 (en) 1993-12-20 1993-12-20 Device for raising the level of a liquid in a cell, well or the like, multi-well plate equipped with this device, and use of this device or this plate for measuring the optical density.

Publications (1)

Publication Number Publication Date
WO1995017661A1 true WO1995017661A1 (en) 1995-06-29

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PCT/FR1994/001467 WO1995017661A1 (en) 1993-12-20 1994-12-14 Device for raising the liquid level in a well for measuring the density

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EP (1) EP0685063A1 (en)
JP (1) JPH08510558A (en)
AU (1) AU1319295A (en)
FR (1) FR2714183B1 (en)
WO (1) WO1995017661A1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2484309C (en) 2004-08-31 2009-11-03 James Samsoondar Sample tab
US7108833B2 (en) 1999-05-12 2006-09-19 Spectromedical Inc. Sample tab
JP5519297B2 (en) * 2007-03-13 2014-06-11 アドヴァンスト リキッド ロジック インコーポレイテッド Droplet actuator apparatus, configuration and method for improved absorbance detection

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2912895A (en) * 1954-03-18 1959-11-17 Hamilton Robert Houston Spectrophotometry process
DE4016617A1 (en) * 1990-05-23 1991-11-28 Rainer Dylla Holder plate e.g. for blood samples - used with structured second plate to form gaps of variable vol. allowing use of small samples
DE4021855A1 (en) * 1990-07-09 1992-01-16 Mfn Milchwirtschaftliche Foerd Cuvette for infrared spectroscopy of pastes or solids - adjusted in depth by spacer polished plates with telescopic guides, for improved measurement

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2912895A (en) * 1954-03-18 1959-11-17 Hamilton Robert Houston Spectrophotometry process
DE4016617A1 (en) * 1990-05-23 1991-11-28 Rainer Dylla Holder plate e.g. for blood samples - used with structured second plate to form gaps of variable vol. allowing use of small samples
DE4021855A1 (en) * 1990-07-09 1992-01-16 Mfn Milchwirtschaftliche Foerd Cuvette for infrared spectroscopy of pastes or solids - adjusted in depth by spacer polished plates with telescopic guides, for improved measurement

Also Published As

Publication number Publication date
FR2714183B1 (en) 1996-03-08
FR2714183A1 (en) 1995-06-23
JPH08510558A (en) 1996-11-05
AU1319295A (en) 1995-07-10
EP0685063A1 (en) 1995-12-06

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