WO1995002691A3 - Production et application de mycelium et de sporophores de champignons transgeniques - Google Patents

Production et application de mycelium et de sporophores de champignons transgeniques Download PDF

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Publication number
WO1995002691A3
WO1995002691A3 PCT/NL1994/000164 NL9400164W WO9502691A3 WO 1995002691 A3 WO1995002691 A3 WO 1995002691A3 NL 9400164 W NL9400164 W NL 9400164W WO 9502691 A3 WO9502691 A3 WO 9502691A3
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WO
WIPO (PCT)
Prior art keywords
transformed
production
donor dna
commercial
fruitbodies
Prior art date
Application number
PCT/NL1994/000164
Other languages
English (en)
Other versions
WO1995002691A2 (fr
Inventor
Andreas Mooibroek
De Rhee Miranda Debora Van
Hindrik Jan Huizing
Franciscus Hendricus Rats
Original Assignee
Inst Voor Agrotech Onderzoek
Cnc Coop Nl Champignonkwekers
Andreas Mooibroek
De Rhee Miranda Debora Van
Hindrik Jan Huizing
Franciscus Hendricus Rats
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Voor Agrotech Onderzoek, Cnc Coop Nl Champignonkwekers, Andreas Mooibroek, De Rhee Miranda Debora Van, Hindrik Jan Huizing, Franciscus Hendricus Rats filed Critical Inst Voor Agrotech Onderzoek
Priority to AU75091/94A priority Critical patent/AU682057B2/en
Priority to EP94925030A priority patent/EP0708826A1/fr
Publication of WO1995002691A2 publication Critical patent/WO1995002691A2/fr
Publication of WO1995002691A3 publication Critical patent/WO1995002691A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/04—Fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne différents procédés de modification de caractéristiques génétiques d'homobasidiomycètes, en particulier, des homobasidiomycètes commercialisables, tel que le champignon commun ou le jeune champignon de couche de l'espèce Agaricus bisporus, au moyen d'un traitement par ADN donneur ou de fusions utilisant des protoplastes, ainsi qu'au moyen de conjugaisons entre des souches. On peut utiliser les procédés pour l'amélioration des caractéristiques commerciales et pour la production commerciale des enzymes et des métabolites. L'invention concerne notamment un procédé d'obtention d'un transformant sélectionnable stable d'un homobasidiomycète pouvant exprimer l'ADN donneur intégré comprenant au moins un marqueur sélectionnable dominant à un niveau détectable, dans lequel ledit hôte est éventuellement non auxotrophe et peut être transformé sans cotransformation par ledit marqueur sélectionnable dominant et est transformé par ledit ADN donneur. L'invention concerne également un procédé de production de sporophores transgéniques stables directement à partir d'hétérocaryons transformés ou indirectement par conjugaison ou fusion protoplastique de transformants obtenus par appariement de transformants obtenus. Elle concerne également un procédé de constitution d'une empreinte génétique de matière à la fois homocaryote et hétécaryote obtenue par transformation, ainsi qu'un procédé de production de matière homocaryote à partir de matière hétérocaryote transformée. Enfin, elle concerne un vecteur spécifique s'utilisant dans la transformation, ainsi qu'un procédé de production dudit vecteur.
PCT/NL1994/000164 1993-07-13 1994-07-13 Production et application de mycelium et de sporophores de champignons transgeniques WO1995002691A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU75091/94A AU682057B2 (en) 1993-07-13 1994-07-13 Production and application of transgenic mushroom mycelium and fruitbodies
EP94925030A EP0708826A1 (fr) 1993-07-13 1994-07-13 Production et application de mycelium et de sporophores de champignons transgeniques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NLPCT/NL93/00149 1993-07-13
NL9300149 1993-07-13

Publications (2)

Publication Number Publication Date
WO1995002691A2 WO1995002691A2 (fr) 1995-01-26
WO1995002691A3 true WO1995002691A3 (fr) 1995-03-09

Family

ID=19861973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL1994/000164 WO1995002691A2 (fr) 1993-07-13 1994-07-13 Production et application de mycelium et de sporophores de champignons transgeniques

Country Status (4)

Country Link
EP (1) EP0708826A1 (fr)
AU (1) AU682057B2 (fr)
CA (1) CA2167152A1 (fr)
WO (1) WO1995002691A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0900027A4 (fr) * 1995-02-09 2000-03-08 Amycel Inc Chapeaux de champignons avec ecaillage reduit
AU5914196A (en) * 1995-06-12 1997-01-09 Proefstation Voor De Champignoncultuur Hydrophobins from edible fungi, genes, nucleotide sequences and dna-fragments encoding for said hydrophobins, and expression thereof
ES2302739T3 (es) 2000-06-28 2008-08-01 The Penn State Research Foundation Metodo para la transformacion del agaricus bisporus.
WO2007111500A1 (fr) * 2006-03-27 2007-10-04 Plant Research International B.V. immunisation par champignons transgeniques
US7973215B2 (en) 2008-04-11 2011-07-05 Mycomagic Biotechnology Co., Ltd. Method for the introduction of a heterologous polynucleotide into a mushroom
US8907165B2 (en) 2009-04-22 2014-12-09 Medicine In Need Corporation Production of provitamin A carotenoids in mushrooms and uses thereof
EP2558566A4 (fr) 2010-04-14 2013-10-02 Penn State Res Found Stratégies de manipulation transgénique de champignons filamenteux
CN110295161B (zh) * 2019-07-16 2023-08-29 华南农业大学 一种巨大口蘑和双孢蘑菇的原生质体融合方法
CN110714094B (zh) * 2019-11-22 2022-06-03 福建农林大学 一种特异性鉴别银耳菌株的scar分子标记及其鉴定方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996390A (en) * 1989-01-19 1991-02-26 Campbell Soup Company Novel interspecific mushroom strains

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5362640A (en) * 1992-03-09 1994-11-08 Oji Paper Co., Ltd. Ornithine carbamoyl transferase gene and utilization of the DNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996390A (en) * 1989-01-19 1991-02-26 Campbell Soup Company Novel interspecific mushroom strains

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
A.S. SONNENBERG ET AL.: "An efficient protoplast/regeneration system for Agaricus bisporus and Agaricus bitorquis", CURRENT MICROBIOLOGY, vol. 17, 1988, SPRINGER-VERLAG, NEW YORK, US, pages 285 - 291 *
H. MOOIBROEK ET AL.: "Introduction of hygromycin B resistance into Schizzophyllum commune: Preferential methylation of donor DNA", MOLECULAR & GENERAL GENETICS, vol. 222, 1990, SPRINGER INTERNATIONAL, NEW YORK, US;, pages 41 - 48 *
L.J.L.D. VAN GRIENSVEN (EDITOR), GENETICS AND BREEDINGS OF AGARICUS, 1991, PUDOC, WAGENINGEN, NETHERLANDS;, pages 129 - 134 *
L.J.L.D. VAN GRIENSVEN (EDITOR), GENETICS AND BREEDINGS OF AGARICUS, 1991, PUDOC, WAGENINGEN, NETHERLANDS;, pages 135 - 139 *
L.J.L.D. VAN GRIENSVEN (EDITOR), GENETICS AND BREEDINGS OF AGARICUS, 1991, PUDOC, WAGENINGEN, NETHERLANDS;, pages 62 - 72 *
M. PENG ET AL.: "Recovery of recombinant plasmids from Pleuterus ostreatus transformant", CURRENT GENETICS, vol. 22, no. 1, July 1992 (1992-07-01), SPRINGER INTERNATIONAL, NEW YORK, US;, pages 53 - 59 *
M.C. HARMSEN ET AL.: "Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidomycetes Schizophillum commune, Phanerochaete chrysosporium and Agaricus bisporus", CURRENT GENETICS, vol. 22, no. 6, December 1992 (1992-12-01), SPRINGER INTERNATIONAL, NEW YORK, US;, pages 447 - 454 *
M.G. MARK: "The application of recombinant DNA technology to the cultivated mushroom", DISSERTATION ABSTRACT INTERNATIONAL B, vol. 49, no. 11, May 1989 (1989-05-01), pages 4693-B *
R.S. KHUSH ET AL.: "DNA amplification polymorphisms of the cultivated mushroom Agaricus bisoprus", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 58, no. 9, September 1992 (1992-09-01), AM.SOC.MICROBIOL.,WASHINGTON,DC,US;, pages 2971 - 2977 *
R.W. KERRIGAN ET AL.: "Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus", GENETICS, vol. 133, no. 2, February 1993 (1993-02-01), GENETICS SOCIETY OF AMERICA, BALTIMORE, MD, US;, pages 225 - 236 *

Also Published As

Publication number Publication date
WO1995002691A2 (fr) 1995-01-26
CA2167152A1 (fr) 1995-01-26
AU7509194A (en) 1995-02-13
EP0708826A1 (fr) 1996-05-01
AU682057B2 (en) 1997-09-18

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