WO1995000625A1

WO1995000625A1 - Granular laundry detergent compositions containing lipase and sodium nonanoyloxybenzene sulfonate

Info

Publication number: WO1995000625A1
Application number: PCT/US1994/006305
Authority: WO
Inventors: Ann Margaret Wolff; Manuel Garcia Venegas
Original assignee: The Procter & Gamble Company
Priority date: 1993-06-25
Filing date: 1994-06-06
Publication date: 1995-01-05
Also published as: JPH08511826A; EP0724623A1; AU7244594A; MA23232A1

Abstract

A granular laundry detergent composition containing lipase produced by Pseudomonas alcaligenes, certain levels of alkylalkoxy sulfate or polyhydroxy fatty acid amide, and sodium nonanoyloxybenzene sulfonate and sodium perborate in a specified ratio for good stain removal and bleaching action is described.

Description

GRANULAR LAUNDRY DETERGENT COMPOSITIONS CONTAINING LIPASE AND SODIUM NONANOYLOXYBENZENE SULFONATE

TECHNICAL FIELD This relates to a granular laundry detergent composition containing lipase produced by Pseudomonas alcaligenes, certain levels of alkyl alkoxy sulfate or polyhydroxy fatty acid amide, and sodium nonanoyloxybenzene sulfonate and sodium perborate in a specified ratio for good stain removal and bleaching action.

BACKGROUND OF THE INVENTION The inclusion of lipase in laundry detergent compositions for improved cleaning performance is known. However, in an activated bleach-containing granular laundry detergent composition, the inclusion of lipase can result in compromised bleach effectiveness. A granular laundry detergent composition with both good stain removal and good bleaching action has now been found. The present composition contains lipase produced by Pseudomonas alcaligenes. certain levels of alkyl alkoxy sulfate or polyhydroxy fatty acid amide, and certain levels of sodium nonanoyloxybenzene sulfonate and sodium perborate in a specified ratio. U.S. Patents 4,861,509 and 4,769,173, both Cornelissen et al, issued August 29, 1989, and September 6, 1988, respectively, describe a certain class of fungal and bacterial lipases together with bleaching agent in detergent compositions.

U. S. Patent 4,933,287, Farin et al , issued June 12, 1990, discloses lipases, including those obtained from Pseudomonas pseudoalcaljqenes, which are useful in washing compositions with detergent additives such as bleach.

SUMMARY OF THE INVENTION This relates to a granular laundry detergent composition, comprising: (a) from about 0.00025 to about 0.015 grams of active enzyme per 100 grams of composition of lipase produced by Pseudomonas alcaligenes;

(b) from about 0.5 to about 30%, by weight of the composition, of alkyl alkoxy sulfate or polyhydroxy fatty acid amide; and

(c) from about 1 to about 20%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate and from about 0.5 to about 20%, by weight of the composition, of sodium perborate; in a ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate of between about 0.5:1 and about 10:1; and wherein the composition has a pH in water at about 20^βC of between about 7 and about 11.

DESCRIPTION OF THE INVENTION

A. Lipase

Granular laundry detergent compositions of the present invention include from about 0.00025 to about 0.015, preferably from about 0.0025 to about 0.01, grams of active enzyme per 100 grams of composition of lipase produced by Pseudomonas alcaligenes. Genetically engineered variants and mutants thereof are also included in this lipase definition, as is lipase obtained from Pseudomonas pseudoalcaligenes (which is believed to be the old name for Pseudomonas alcaligenes).

Most preferred is from about 0.05 to about 0.2%, by weight of the composition, of lipase produced by Pseudomonas alcaligenes at

200,000 Lipase Units per gram. A lipase unit (LU) is defined as the amount of lipase which produces 1 umol of titratable butyric acid per minute in a pH stat, where pH is 7.0, temperature is

30*C, and substrate is an emulsion of tributyrin and gum arabic in the presence of Ca++ and NaCl in phosphate buffer.

Lipase produced by Pseudomonas alcaligenes is described in

U. S. Patent 4,933,287, Farin et al , issued June 12, 1990, which is incorporated herein by reference. B. Alkyl Alkoxy Sulfate or Polvhvdroxy Fattv Acid Amide

Granular laundry detergent compositions of the present invention further include from about 0.5 to about 30%, preferably from about 1 to about 10%, most preferably from about 1 to about 3%, by weight of the composition, of alkyl alkoxy sulfate or polyhydroxy fatty acid. Mixtures of the two are included herein.

1. Alkyl Alkoxy Sulfate

Preferred is from about 1 to about 10%, most preferably from about 1 to about 3%, by weight of the composition, of C10-24, preferably C12-18, alkyl sulfate which has been ethoxylated with an average of from about 0.5 to about 6, preferably from about 1 to about 3, moles of ethylene oxide per mole of sulfate. Alkyl alkoxy(lated) sulfate surfactants are water soluble salts or acids typically of the formula RO(A)_mS03M wherein R is an unsubstituted ^c10^_c24 alkyl or hydroxyalkyl group having a C10-C24 alkyl component, preferably a C12-C20 alkyl or hydroxyalkyl, more preferably C12-C18 alkyl or hydroxyalkyl, A is an ethoxy or propoxy unit, m is greater than zero, typically between about 0.5 and about 6, more preferably between about 0.5 and about 3, and M is H or a cation which can be, for example, a metal cation (e.g., sodium, potassium, lithium, calcium, magnesium), ammonium or substituted-ammonium cation.

Alkyl ethoxylated sulfates as well as alkyl propoxylated sulfates are contemplated herein. Specific examples of substituted ammonium cations include methyl-, dimethyl-, tri ethyl-ammonium and quaternary ammonium cations, such as tetra ethyl-ammonium, dimethyl piperydinium and cations derived from al anolamines, e.g. monoethanolamine, diethanola ine, and triethanol mine, and mixtures thereof. Exemplary surfactants are ^C12^-C18 alkyl polyethoxylate (1.0) sulfate, C12-C18 alkyl polyethoxylate (2.25) sulfate, C12-C18 alkyl polyethoxylate (3.0) sulfate, and Ci2^_c18 alkyl polyethoxylate (4.0) sulfate wherein M is selected from sodium and potassium.

2. Polvhvdroxy Fatty Acid Amide Polyhydroxy fatty acid amide compri ses compounds of the structural formula: Rl

(I) R2 - C - N - Z wherein: R¹ is H, C1-C4 hydrocarbyl, 2-hydroxy ethyl, 2-hydroxy propyl , or a mixture thereof, preferably C1-C4 alkyl, more preferably Ci or C2 alkyl, most preferably Ci alkyl (i.e., methyl); and R² is a C5-C31 hydrocarbyl, preferably straight chain C7-C19 alkyl or alkenyl, more preferably straight chain C9-C17 alkyl or alkenyl, most preferably straight chain C11-C15 alkyl or alkenyl, or mixtures thereof; and Z is a polyhydroxyhydrocarbyl having a linear hydrocarbyl chain with at least 3 hydroxyls directly connected to the chain, or an alkoxylated derivative (preferably ethoxylated or propoxylated) thereof. Z preferably will be derived from a reducing sugar in a reductive amination reaction; more preferably Z will be a glycityl. Suitable reducing sugars include glucose, fructose, maltose, lactose, galactose, annose, and xylose. Z preferably will be selected from the group consisting of -CH2-(CHOH)_n-CH2θH, -CH(CH20H)-(CH0H)_n-i-CH20H, -CH2-(CHOH)2(CHOR')(CHOH)-CH2θH, and alkoxylated derivatives thereof, where n is an integer from 3 to 5, inclusive, and R' is H or a cyclic or aliphatic monosaccharide. Most preferred are glycityls wherein n is 4, particularly -CH2-(CHOH)4-CH2θH.

The polyhydroxy fatty acid amide preferred herein is glucose amide, preferably C12-I8 N-acetyl glucamide.

C. Sodium nonanoyloxybenzene sulfonate and Sodium perborate Granular laundry detergent compositions of the present invention further include from about 1 to about 20%, preferably from about 2 to about 15%, most preferably from about 5 to about 10%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate and from about 0.5 to about 20%, preferably from about 2 to about 15%, most preferably from about 4 to about 8%, by weight of the composition, of sodium perborate. The ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate is between about 0.5:1 and about 10:1, preferably between about 1:1 and about 5:1, most preferably between about 1:1 and about 2:1. These components are described in U.S. Patent 4,412,934, Chung et al , issued November 1, 1983, which is incorporated herein by reference. The present composition has a pH in water at about 20^βC of between about 7 and about 11, preferably between about 8 and about 10.

D. Additional Optional Ingredients 1. Anionic Surfactant

The compositions herein optionally further comprise from about 2 to about 30%, preferably from about 10 to about 20%, by weight of the composition, of additional anionic or nonionic detergent surfactant.

Anionic surfactants useful for detersive purposes are preferably included in the compositions hereof. These can include salts (including, for example, sodium, potassium, ammonium, and substituted ammonium salts such as mono-, di- and triethanolamine salts) of soap, C9-C20 linear alkylbenzenesulphonates, C8-C22 primary or secondary alkanesulphonates, C8-C24 olefinsulphonates, sulphonated polycarboxylic acids prepared by sulphonation of the pyrolyzed product of alkaline earth metal citrates, e.g., as described in British Patent Specification No. 1,082,179, alkyl glycerol sulfonates, fatty acyl glycerol sulfonates, fatty oleyl glycerol sulfates, alkyl phenol ethylene oxide ether sulfates, paraffin sulfonates, alkyl phosphates, isothionates such as the acyl isothionates, N-acyl taurates, fatty acid amides of methyl tauride, alkyl succinamates and sulfosuccinates, monoesters of sulfosuccinate (especially saturated and unsaturated C1 -C18 monoesters) diesters of sulfosuccinate (especially saturated and unsaturated C6-C14 diesters), N-acyl sarcosinates, sulfates of alkylpolysaccharides such as the sulfates of alkylpolyglucoside (the nonionic nonsulfated compounds being described below), branched primary alkyl sulfates, alkyl polyethoxy carboxylates such as those of the formula RO(CH2CH2θ)kCH2COO"M⁺ wherein R is a C8-C22 alkyl, k is an integer from 0 to 10, and M is a soluble salt-forming cation, and fatty acids esterified with isothionic acid and neutralized with sodium hydroxide. Resin acids and hydrogenated resin acids are also suitable. Further examples are given in "Surface Active Agents and Detergents" (Vol. I and II by Schwartz, Perry and Berch). A variety of such surfactants are also generally disclosed in U.S. Patent 3,929,678, issued December 30, 1975 to Laugh!in, et al . at Column 23, line 58 through Column 29, line 23 (herein incorporated by reference).

Preferred for use herein are C12-C20 alkyl sulfate, and/or C9-C20 linear alkylbenzene sulfonate (preferably sodium salts). Preferred are from about 2 to about 10%, by weight of the composition, of sodium Cj2-20 alkyl sulfate and from about 5 to about 15%, by weight of the composition, of sodium C9-20 linear alkylbenzene sulfonate. 2. Nonionic Surfactant

Suitable nonionic detergent surfactants are generally disclosed in U.S. Patent 3,929,678, Laughlin et al., issued December 30, 1975, at column 13, line 14 through column 16, line 6, incorporated herein by reference. Exemplary, non-limiting classes of useful nonionic surfactants are listed below. 1. The polyethylene, polypropylene, and polybutylene oxide condensates of alkyl phenols. In general, the polyethylene oxide condensates, particularly alkyl phenol ethoxylates, are preferred.

2. The condensation products of ethylene oxide with a hydrophobic base formed by the condensation of propylene oxide with propylene glycol.

3. The condensation products of ethylene oxide with the product resulting from the reaction of propylene oxide and ethylenediamine.

4. Semi-polar nonionic surfactants are a special category of nonionic surfactants which include water-soluble amine oxides containing one alkyl moiety of from about 10 to about 18 carbon atoms and 2 moieties selected from the group consisting of alkyl groups and hydroxyalkyl groups containing from about 1 to about 3 carbon atoms; water-soluble phosphine oxides containing one alkyl moiety of from about 10 to about 18 carbon atoms and 2 moieties selected from the group consisting of alkyl groups and hydroxyalkyl groups containing from about 1 to about 3 carbon atoms; and water-soluble sulfoxides containing one alkyl moiety of from about 10 to about 18 carbon atoms and a moiety selected from the group consisting of alkyl and hydroxyalkyl moieties of from about 1 to about 3 carbon atoms. 5. Alkylpolysaccharides disclosed in U.S. Patent 4,565,647, Llenado, issued January 21, 1986, having a hydrophobic group containing from about 6 to about 30 carbon atoms, preferably from about 10 to about 16 carbon atoms and a polysaccharide, e.g., a polyglycoside, hydrophilic group containing from about 1.3 to about 10 saccharide units.

6. Fatty acid amide surfactants having the formula:

0

II

wherein R⁶ is an alkyl group containing from about 7 to about 21 (preferably from about 9 to about 17) carbon atoms and each R? is selected from the group consisting of hydrogen C1-C4 alkyl, C1-C4 hydroxyalkyl, and -(C2H4θ)_xH where x varies from about 1 to about 3. 3. Detergency Builder

From 1 to about 80, preferably from about 20 to about 70, weight % of inorganic and/or organic detergency builder can optionally be included herein. Inorganic detergency builders include, but are not limited to, the alkali metal, ammonium and alkanolammonium salts of polyphosphates (exemplified by the tripolyphosphates, pyrophosphates, and glassy polymeric meta-phosphates), phosphonates, phytic acid, silicates such as the alkali metal silicates and the layered sodium silicates described in U.S. Patent 4,664,839, carbonates (including bicarbonates and sesquicarbonates), borates, sulphates, and aluminosilicates.

Examples of carbonate builders are the alkaline earth and alkali metal carbonates, including sodium carbonate and sesqui arbonate and mixtures thereof with ultra-fine calcium carbonate as disclosed in German Patent Application No. 2,321,001 published on November 15, 1973. Preferred aluminosilicates are zeolite builders which have the formula:

Na_z[(A10₂)_z (Si0₂)y]-xH₂0 wherein z and y are integers of at least 6, the molar ratio of z to y is in the range from 1.0 to about 0.5, and x is an integer from about 15 to about 264. Preferred synthetic crystalline aluminosilicate ion exchange materials useful herein are available under the designations Zeolite A, Zeolite P (B), and Zeolite X.

Specific examples of polyphosphates are the alkali metal tripolyphosphates, sodium, potassium and ammonium pyrophosphate, sodium and potassium and ammonium pyrophosphate, sodium and potassium orthophosphate, sodium polymeta phosphate in which the degree of polymerization ranges from about 6 to about 21, and salts of phytic acid.

Examples of phosphonate builder salts are the water-soluble salts of ethane 1-hydroxy-l, 1-diphosphonate, the water-soluble salts- of methylene diphosphonic acid e.g. the trisodium and tripotassiu salts and the water-soluble salts of substituted methylene diphosphonic acids, such as the trisodium and tripotassium ethylidene, isopyropylidene benzylmethylidene and halo methylidene phosphonates. Phosphonate builder salts of the aforementioned types are disclosed in U.S. Patents 3,159,581 and 3,213,030 issued December 1, 1964 and October 19, 1965, to Diehl; U.S. Patent 3,422,021 issued January 14, 1969, to Roy; and U.S. Patents 3,400,148 and 3,422,137 issued September 3, 1968, and January 14, 1969 to Quimby, each incorporated herein by reference.

Included among the polycarboxylate builders are the ether polycarboxylates. Examples of useful ether polycarboxylates include oxydisuccinate, as disclosed in Berg, U.S. Patent

3,128,287, issued April 7, 1964, and Lamberti et al., U.S. Patent 3,635,830, issued January 18, 1972, both of which are incorporated herein by reference. A specific type of ether polycarboxylates useful in the present invention is disclosed in U.S. Patent 4,663,071, issued to Bush et al . , on May 5, 1987.

Suitable ether polycarboxylates also include cyclic compounds, particularly alicyclic compounds, such as those described in U.S. Patents 3,923,679; 3,835,163; 4,158,635; 4,120,874 and 4,102,903, all of which are incorporated herein by reference. Other useful detergency builders include the ether hydroxypolycarboxylates and the copolymers of maleic anhydride with ethylene or vinyl methyl ether, 1, 3, 5-trihydroxy benzene-2, 4, 6-trisulphonic acid, and carboxymethyloxysuccinic acid. Organic polycarboxylate builders also include the various alkali metal, ammonium and substituted ammonium salts of polyacetic acids. Examples include the sodium, potassium, lithium, ammonium and substituted ammonium salts of ethylenediamine tetraacetic acid, and nitrilotriacetic acid.

Also included are polycarboxylates such as mellitic acid, succinic acid, oxydisuccinic acid, polymaleic acid, benzene 1,3,5-tricarboxylic acid, and carboxymethyloxysuccinic acid, and soluble salts thereof. Citrate builders, e.g., citric acid and soluble salts thereof (particularly sodium salt), are polycarboxylate builders which can also be used in granular compositions. Other carboxylate builders include the carboxylated carbohydrates disclosed in U.S. Patent 3,723_>322, Diehl, issued March 28, 1973, incorporated herein by reference. Also suitable in the detergent compositions of the present invention are the 3,3-dicarboxy-4-oxa-l,6-hexanedioates and the related compounds disclosed in U.S. Patent 4,566,984, Bush, issued January 28, 1986, incorporated herein by reference. Useful succinic acid builders include the C5-C20 alkyl succinic acids and salts thereof.

Examples of useful builders also include sodium and potassium carboxy ethyloxymalonate, carboxymethyloxysuccinate, cis-cyclo- hexane-hexacarboxylate, cis-cyclopentane-tetracarboxylate, water- soluble polyacrylates (these polyacrylates having molecular weights to above about 2,000 can also be effecitvly utilized as dispersants), and the copoly ers of maleic anhydride with vinyl methyl ether or ethylene.

Other suitable polycarboxylates are the polyacetal carboxylates disclosed in U.S. Patent 4,144,226, Crutchfield et al., issued March 13, 1979, incorporated herein by reference. Polycarboxylate builders are also disclosed in U.S. Patent 3,308,067, Diehl, issued March 7, 1967, incorporated herein by reference. Other organic builders known in the art can also be used. Preferably the detergency builder herein is selected from the group consisting of the salts, preferably the sodium salt, of carbonate, silicate, sulfate, phosphate, aluminosilicate, and citric acid and mixtures thereof. 4. Second Enzyme

Optional, and preferred, ingredients include second enzymes, which include protease (most preferred), amylase, peroxidase, cellulase, and mixtures thereof. By "second enzyme" is meant detergent-compatible enzymes in addition to lipase which are also added to the composition. Second enzymes from chemically or genetically modified mutants, and from bacterial or fungal origin, are included herein. Additional lipases (from sources other than Pseudomonas alcaligenes) could be included herein, but preferably are not.

The amount of second enzyme used in the- composition varies according to the type of enzyme. In general, from about 0.0001 to about 1.0, more preferably about 0.001 to about 0.5, weight % on an active basis of these second enzymes are preferably used. Mixtures of enzymes from the same class (e.g. protease) or two or more classes (e.g. cellulase and protease) may be used.

Suitable cellulases are disclosed in U.S. Patent 4,435,307, Barbesgaard et al., issued March 6, 1984, incorporated herein by reference, which discloses fungal cellulase produced from Humicola insolens. Suitable cellulases are also disclosed in GB-A-2.075.028, GB-A-2.095.275 and DE-0S-2.247.832. Examples are cellulases produced by a strain of Humicola insolens (Humicola grisea var. thermoidea). particularly the Humicola strain DSM 1800, and cellulases produced by a fungus of Bacillus N or a cellulase 212-producing fungus belonging to the genus Aeromonas, and cellulase extracted from the hepatopancreas of a marine mollusc (Dolabella Auricula Solander). Suitable a ylases include, for example, α-amylases obtained from a special strain of B.licheniforms, described in more detail in British Patent Specification No. 1,296,839. Amylolytic proteins include, for example, Rapidase™, Maxamyl and TermamylTM. Peroxidase enzymes are used in combination with oxygen sources, e.g., percarbonate, perborate, persulfate, hydrogen peroxide, etc. They are known in the art, and include, for example, horseradish peroxidase, ligninase, and haloperoxidase such as chloro-and bro o-peroxidase. Peroxidase-containing detergent compositions are disclosed, for example, in PCT International Application WO 89/099813, published October 19, 1989, by 0. Kirk, assigned to Novo Industries A/S, incorporated herein by reference.

From about 0.0001 to about 1.0, preferably about 0.0005 to about 0.5, most preferably about 0.002 to about 0.1, weight % on an active basis of detergent-compatible protease is preferred for use herein. Mixtures of protease enzymes are also included. The protease can be of animal, vegetable or microorganism (preferred) origin. More preferred is serine protease enzyme of bacterial origin. Purified or nonpurified forms of this, enzyme may be used. Proteases produced by chemically or genetically modified mutants are included by definition, as are close structural enzyme variants. Particularly preferred is bacterial serine protease enzyme obtained from Bacillus subtilis and/or Bacillus licheniformis.

Suitable proteases include Alcalase^®, Esperase®, Savinase^® (preferred); Maxatase^®, Maxacal® (preferred), and Maxapem 15® (protein engineered Maxacal^®); and subtilisin BPN and BPN' (preferred); which are commercially available. Preferred proteases are also modified bacterial serine proteases, such as those described in European Patent Application Serial Number 87 303761.8, filed April 28, 1987 (particularly pages 17, 24 and 98), and which is called herein "Protease B", and in European Patent Application 199,404, Venegas, published October 29, 1986, which refers to a modified bacterial serine proteolytic enzyme which is called "Protease A" herein. Protease B, and genetically engineered variants and mutants thereof, are most preferred for use herein. 5. Other Ingredients

Other ingredients suitable for use in the present compositions, such as water, perfume, brightener, conditioners such as fumed silica, polyethylene glycol, dyes and colorants, and peroxyacids, can be included. Preferred ingredients are from about 0.5 to about 5%, by weight of the composition, of 12 -

polyethylene glycol (preferably with molecular weight between 5,000 and 10,000, most preferably 8,000), from about 0.01 to about 0.7 wt.% of fluorescent whitening and/or brightening agents, and from about 0.01 to about 1.0 wt.% of perfume. 5 E. Method for Cleaning Fabrics

This invention further provides a method for cleaning fabrics in the wash by contacting the fabrics with effective amounts of a granular laundry detergent composition comprising:

(a) from about 0.00025 to about 0.015, preferably from about 0 0.0025 to about 0.01, grams of active enzyme per 100 grams of composition of lipase produced by Pseudomonas alcaligenes:

(b) from about 0.5 to about 30%, preferably from about 1 to about 10%, by weight of the composition, of alkyl alkoxy 5 sulfate or polyhydroxy fatty acid amide; and

(c) from about 1 to about 20%, preferably from about 2 to about 15%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate, and from about 0.5 to about 20%, preferably from about 2 to about 15%, by o weight of the composition, of sodium perborate; in a ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate of between about 0.5:1 and about 10:1, preferably between about 1:1 and about 5:1; and wherein the composition has a pH in water at about 20^*C of 5 between about 7 and about 11.

The granular detergent composition is added to the wash, usually at levels of 1/8 to 1 cup. Agitation is preferably provided in the washing machine for good cleaning. Washing is preferably followed by drying the wet fabric in a conventional 0 clothes dryer. An effective amount of a granular detergent composition is preferably from about 500 to about 7000 ppm, more preferably from about 1000 to about 3000 ppm.

The following examples illustrate the compositions of the present invention, but are not necessarily meant to limit or 5 otherwise define the scope of the invention.

All parts, percentages and ratios used herein are by weight unless otherwise specified. EXAMPLE I A composition of the present invention is as follows, detergent base having the following composition is spray dried.

Component Weight %

Sodium C12-13 linear alkylbenzene sulfonate 10.36

Sodium Ci4-i5 alkyl sulfate 4.44

Alkyl ethoxy (El) sulfate 2.0

Sodium carbonate 8.74

Sodium aluminosilicate (Zeolite A) 21.30

Sodium silicate (1.6R) 1.85

Sodium diethylene triamine pentacetate 0.43

Polyethylene glycol (molecular weight 8000) 1.05

Sodium polyacrylate 2.72

Sodium sulfate 8.21

Water, perfume, miscellaneous Balance

The ingredients below are admixed with the spray dried particles of the detergent base above. The perfume is sprayed onto the particles after the other ingredients are added. Admix/Spray On Weight %

Sodium carbonate 16.00

Citric acid 3.00

Sodium perborate 5.00

Sodium nonanoyloxybenzene sulfonate 7.42

Perfume 0.25

Protease enzyme (34 mg/g)* 0.75

Lipase enzyme** 0.145

* Modified bacterial serine protease described in European Patent Application Serial No. 87303761.8, filed April 28, 1987, which is called Protease B herein.

** Lipase produced by Pseudomonas alcaligenes (M-l from Gist-brocades, Delft, Holland) at 200,000 Lipase Units per gram. The pH of the composition in water at about 20^βC is 9.5. C12-18 N-acetyl glucosamide can be substituted for or added to the alkyl ethoxy sulfate. Variants or mutants of the lipase produced by Ei alcaligenes can be substituted for or used with the lipase produced by J\. alcaligenes.

EXAMPLE II A composition of the present invention is as follows, detergent base having the following formula is spray dried. Component Weight %

Sodium 12-13 linear alkyl benzene sulfonate 14.3 Sodium C14-15 alkyl sulfate 4.1 Phosphate solids 34.0 Sodium carbonate 0.3 Sodium silicate (2.Or) 6.5 Polyethylene glycol (MW 8000) 0.8 Alkyl ethoxy (El) sulfate 3.0 Ci2-15 Alcohol polyethoxylate (E9) 0.5 Water, perfume, sodium sulfate, miscellaneous Balance

The ingredients below are admixed with the spray dried particles described above. Admix:

Sodium carbonate 8.4

Sodium nonanoyloxybenzene sulfonate 8.0

Sodium perborate 4.0

Lipase enzyme (200,000 LU/g) 0.3

Protease enzyme (34 mg/g) 0.5

Silicone 0.2

C12-18 N-acetyl glucosamide can be substituted for or added to the alkyl ethoxy sulfate. Variants or mutants of the lipase produced by Ei alcaligenes can be substituted for or used with the lipase produced by E-_s. alcaligenes. EXAMPLE III

A composition of the present invention is as follows, detergent base having the following formula is spray dried.

Component Weight %

C12-14 N-acetyl glucosamide 4.3

Sodium C14-15 alkyl sulfate 13.0

Phosphate solids 38.0

Sodium carbonate 0.3

Sodium silicate (2.Or) 6.5

Polyethylene glycol (MW 8000) 0.8

Alkyl ethoxy (El) sulfate 4.3

C12-15 Alcohol polyethoxylate (E9) 0.5

Water, perfume, miscellaneous ' Balance

Sodium carbonate 8.4

Sodium nonanoyloxybenzene sulfonate 6.0 Sodium perborate 4.0 Lipase enzyme (200,000 LU/g) 0.3 Protease enzyme (34 mg/g) 0.5 Silicone 0.2

C12-18 N-acetyl glucosamide can be substituted for or added to the alkyl ethoxy sulfate. Variants or mutants of the lipase produced by E_a. alcaligenes can be substituted for or used with the lipase produced by E-. alcaligenes.

Claims

What is claimed is:

1. A granular laundry detergent composition, comprising:

(a) from 0.00025 to 0.015 grams of active enzyme per 100 grams of composition of lipase produced by Pseudomonas alcaligenes:

(b) from 0.5 to 30%, by weight of the composition, of alkyl alkoxy sulfate or polyhydroxy fatty acid amide; and

(c) from 1 to 20%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate and from 0.5 to 20%, by weight of the composition, of sodium perborate; in a ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate of between 0.5: 1 and 10:1; and wherein the composition has a pH in water at 20°C of between 7 and 11.

2. A granular laundry detergent composition according to Claim 1, comprising from 1 to 10%, by weight of the composition, of polyhydroxy fatty acid amide.

3. A granular laundry detergent composition according to Claim 1, comprising from 0.0025 to 0.01 grams of active enzyme per 100 grams of composition of lipase produced by Pseudomonas alcaligenes.

4. A granular laundry detergent composition according to Claim 3, comprising from 1 to 10%, by weight of the composition, of C10-24 alkyl sulfate which has been ethoxylated with an average of from 0.5 to 6 moles of ethylene oxide per mole of sulfate; from 2 to 15%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate; from 2 to 15%, by weight of the composition, of sodium perborate; and from 2 to 30%, by weight of the composition, of additional anionic or detergent nonionic surfactant.

5. A granular laundry detergent composition according to Claim 2, wherein the ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate is between 1: 1 and 5:1; and wherein said composition comprises from 1 to 3%, by weight of the composition, of polyhydroxy fatty acid amide.

6. A granular laundry detergent composition according to Claim 4, further comprising from 0.0001 to 1.0 weight % on an active basis of detergent- compatible protease; from 2 to 10%, by weight of the composition, of sodium Cj2-20 ^^ sulfate; and from 5 to 15%, by weight of the composition, of sodium C9.20 linear alkylbenzene sulfonate.

7. A granular laundry detergent composition according to Claim 6, comprising from 4 to 10%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate and from 4 to 8%, by weight of the composition, of sodium perborate.

8. A granular laundry detergent composition according to Claim 6, wherein the ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate is between 1:1 and 2: 1.

9. A granular laundry detergent composition according to Claim 8, comprising from 1 to 3%, by weight of the composition, of C12-18 alkyl sulfate which has been ethoxylated with an average of from 1 to 3 moles of ethylene oxide per mole of sulfate.

10. A granular laundry detergent composition according to Claim 9, comprising from 1 to 3%, by weight of the composition, of glucosamide; from 20 to 70%, by weight of the composition, of detergency builder selected from the sodium salts of carbonate, silicate, sulfate, phosphate, aluminosilicate, and citric acid and mixtures thereof.

11. A granular laundry detergent composition according to Claim 10, comprising from 0.5 to 5%, by weight of the composition, of polyethylene glycol with a molecular weight between 5,000 and 10,000.

12. A method for cleaning fabrics in the wash by contacting the fabrics with effective amounts of a granular laundry detergent composition comprising:

(c) from 1 to 20%, by weight of the composition, of sodium nonanoyloxybenzene sulfonate and from 0.5 to 20%, by weight of the composition, of sodium perborate; in a ratio of sodium nonanoyloxybenzene sulfonate to sodium perborate of between 0.5: 1 and 10: 1; and wherein the composition has a pH in water at 20°C of between 7 and 11.