WO1994028146A3 - Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee - Google Patents

Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee Download PDF

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Publication number
WO1994028146A3
WO1994028146A3 PCT/EP1994/001671 EP9401671W WO9428146A3 WO 1994028146 A3 WO1994028146 A3 WO 1994028146A3 EP 9401671 W EP9401671 W EP 9401671W WO 9428146 A3 WO9428146 A3 WO 9428146A3
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WIPO (PCT)
Prior art keywords
dna sequences
plasmids
sugar beet
preparation
sucrose concentration
Prior art date
Application number
PCT/EP1994/001671
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English (en)
Other versions
WO1994028146A2 (fr
Inventor
Holger Hesse
Bernd Mueller-Roeber
Original Assignee
Hoechst Schering Agrevo Gmbh
Holger Hesse
Mueller Roeber Bernd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4317596A external-priority patent/DE4317596A1/de
Application filed by Hoechst Schering Agrevo Gmbh, Holger Hesse, Mueller Roeber Bernd filed Critical Hoechst Schering Agrevo Gmbh
Priority to EP94916985A priority Critical patent/EP0701617A1/fr
Priority to PCT/EP1994/001671 priority patent/WO1994028146A2/fr
Priority to US08/553,436 priority patent/US5866790A/en
Publication of WO1994028146A2 publication Critical patent/WO1994028146A2/fr
Publication of WO1994028146A3 publication Critical patent/WO1994028146A3/fr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne des séquences d'ADN et des plasmides qui modifient la concentration de sucrose lorsqu'ils sont incorporés dans un génome de la betterave, ainsi que des plantes transgéniques présentant des modifications de la concentration de sucre, obtenues par l'introduction des séquences d'ADN selon l'invention.
PCT/EP1994/001671 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee WO1994028146A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP94916985A EP0701617A1 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee
PCT/EP1994/001671 WO1994028146A2 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee
US08/553,436 US5866790A (en) 1993-05-24 1994-05-20 DNA sequences and plasmids for the preparation of sugar beet with changed sucrose concentration

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DEP4317596.1 1993-05-24
DE4317596A DE4317596A1 (de) 1993-05-24 1993-05-24 DNA-Sequenzen und Plasmide zur Herstellung von Zuckerrüben mit veränderter Saccharose-Konzentration
PCT/EP1994/001671 WO1994028146A2 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee

Publications (2)

Publication Number Publication Date
WO1994028146A2 WO1994028146A2 (fr) 1994-12-08
WO1994028146A3 true WO1994028146A3 (fr) 1995-01-26

Family

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Application Number Title Priority Date Filing Date
PCT/EP1994/001671 WO1994028146A2 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee

Country Status (1)

Country Link
WO (1) WO1994028146A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU644619B2 (en) 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
US5981852A (en) * 1990-07-20 1999-11-09 Calgene Llc Modification of sucrose phosphate synthase in plants
GB9412018D0 (en) 1994-06-16 1994-08-03 Cambridge Advanced Tech Modification of starch content in plants
US5750869A (en) * 1995-01-15 1998-05-12 Calgene, Inc. Soluble solids modification using sucrose phosphate synthase encoding sequences
US6124528A (en) * 1995-01-15 2000-09-26 Calgene Llc Modification of soluble solids in fruit using sucrose phosphate synthase encoding sequence
EP0858513A2 (fr) * 1995-10-27 1998-08-19 Calgene, Inc. Modification de solides solubles a l'aide d'une sequence codant la phosphate synthase de saccharose
DE19607697C2 (de) * 1996-02-29 1998-04-09 Max Planck Gesellschaft Speicherwurzelgewebespezifisches Regulon der Zuckerrübe
US6682918B1 (en) 1996-07-19 2004-01-27 Arch Development Corporation Bacterial sucrose synthase compositions and methods of use
ZA989782B (en) * 1997-10-30 1999-05-04 Mogen Int Pre-and postharvest inhibition of remobilisation of storage compounds
DE19836098A1 (de) 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
BR9914437A (pt) * 1998-10-13 2001-10-16 Genesis Res & Dev Corp Ltd Materiais e métodos para a modificação de polissacarìdeos de parede celular de planta
DE10313795A1 (de) * 2003-03-20 2004-10-07 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Veränderte PPase-Expression in Zuckerrübe
ES2245867B1 (es) 2004-02-05 2007-04-01 Universidad Publica De Navarra Procedimiento produccion sacarosa sintasa recombinante a partir de escherichia coli y uso en fabricacion de kits de determinacion de sacarosa, produccion de azucares-nucleotidos y obtencion de plantas transgenicas con alto contenido de almidon y alto balance amilosa/amilopectina.
US8076534B2 (en) 2005-07-22 2011-12-13 Syngenta Participations Ag Chlamydomonas glucan dikinase gene, enzyme and modified starch, uses, methods for production thereof
SE537679C2 (sv) * 2013-08-29 2015-09-29 Sveriges Stärkelseproduct Förening Upa Genetisk modifierad Beta vulgaris
AU2015213254A1 (en) * 2014-01-29 2017-08-17 The University Of Queensland Yield promoter to increase sucrose and sucrose derivatives in plants

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438904A1 (fr) * 1989-12-21 1991-07-31 Advanced Technologies (Cambridge) Limited Modification du métabolisme végétal
EP0455316A2 (fr) * 1990-04-20 1991-11-06 Institut Für Genbiologische Forschung Berlin Gmbh Plasmides contenant des séquences d'ADN entraînant la modification de la concentration et de la composition en hydrates de carbones et en protéines dans les plantes, ainsi que des cellules de plantes et des plantes contenant ces plasmides
WO1991019806A1 (fr) * 1990-06-18 1991-12-26 Monsanto Company Plantes a teneur en amidon augmentee
EP0466995A2 (fr) * 1990-07-20 1992-01-22 Roussel Uclaf Saccharose phosphate Synthétase (SPS), son procédé de préparation, son ADNc et utilisation de l'ADNc pour modifier l'expression de la SPS dans les cellules végétales
WO1992016631A1 (fr) * 1991-03-18 1992-10-01 Roussel-Uclaf SYNTHASE DE PHOSPHATE DE SUCROSE (SPS), PROCEDE DE PREPARATION, SON ADNc ET UTILISATION DE L'ADNc POUR MODIFIER L'EXPRESSION DE SPS DANS LES CELLULES VEGETALES
EP0530978A2 (fr) * 1991-08-08 1993-03-10 Advanced Technologies (Cambridge) Limited Modification de l'accumulation de saccharose
WO1993009237A1 (fr) * 1991-11-05 1993-05-13 Sandoz Ltd. Mais tres sucre et ameliore

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438904A1 (fr) * 1989-12-21 1991-07-31 Advanced Technologies (Cambridge) Limited Modification du métabolisme végétal
EP0455316A2 (fr) * 1990-04-20 1991-11-06 Institut Für Genbiologische Forschung Berlin Gmbh Plasmides contenant des séquences d'ADN entraînant la modification de la concentration et de la composition en hydrates de carbones et en protéines dans les plantes, ainsi que des cellules de plantes et des plantes contenant ces plasmides
WO1991019806A1 (fr) * 1990-06-18 1991-12-26 Monsanto Company Plantes a teneur en amidon augmentee
EP0466995A2 (fr) * 1990-07-20 1992-01-22 Roussel Uclaf Saccharose phosphate Synthétase (SPS), son procédé de préparation, son ADNc et utilisation de l'ADNc pour modifier l'expression de la SPS dans les cellules végétales
WO1992016631A1 (fr) * 1991-03-18 1992-10-01 Roussel-Uclaf SYNTHASE DE PHOSPHATE DE SUCROSE (SPS), PROCEDE DE PREPARATION, SON ADNc ET UTILISATION DE L'ADNc POUR MODIFIER L'EXPRESSION DE SPS DANS LES CELLULES VEGETALES
EP0530978A2 (fr) * 1991-08-08 1993-03-10 Advanced Technologies (Cambridge) Limited Modification de l'accumulation de saccharose
WO1993009237A1 (fr) * 1991-11-05 1993-05-13 Sandoz Ltd. Mais tres sucre et ameliore

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BIOLOGICAL ABSTRACTS, vol. 85, Philadelphia, PA, US; abstract no. 72586, FIEUW, S., ET AL.: "Sucrose synthase and sucrose phosphate synthase in sugar beet plants Beta-vulgaris -ssp-altissima" *
BIOLOGICAL ABSTRACTS, vol. 95, Philadelphia, PA, US; abstract no. 79002, SAKALO, V.D., ET AL.: "Characterization of molecular forms of sucrose synthase from beet roots" *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; MURAKAMI, T.: "Histochemical loclaization of UDPG ADPG-pyrophosphorylase and phosphorylase in young petiole of sugar beet Beta-vulgaris L." *
EMBL SEQUENCE DATABASE ACC. NO. S54379 Sucrose phosphate synthase release 35; 31-MAR-1993 *
FIZIOL RAST (MOSC), vol. 39, no. 2, 1992, pages 290 - 299 *
J. HISTOCHEM. CYTOCHEM., vol. 36, no. 7A, 1988, pages 927 *
J. PLANT PHYSIOL., vol. 131, no. 1-2, 1987, pages 153 - 162 *
SALANOUBAT, M., ET AL.: "Molecular cloning and sequencing of sucrose synthase cDNA from potato (Solanum tuberosum L.): preliminary characterization of sucrose synthase mRNA distribution", GENE, vol. 60, 1987, AMSTERDAM NL, pages 47 - 56, XP023544732, DOI: doi:10.1016/0378-1119(87)90212-5 *

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