WO1994028146A2 - Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee - Google Patents

Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee Download PDF

Info

Publication number
WO1994028146A2
WO1994028146A2 PCT/EP1994/001671 EP9401671W WO9428146A2 WO 1994028146 A2 WO1994028146 A2 WO 1994028146A2 EP 9401671 W EP9401671 W EP 9401671W WO 9428146 A2 WO9428146 A2 WO 9428146A2
Authority
WO
WIPO (PCT)
Prior art keywords
leu
ser
gly
glu
val
Prior art date
Application number
PCT/EP1994/001671
Other languages
English (en)
Other versions
WO1994028146A3 (fr
Inventor
Holger Hesse
Bernd Müller-Röber
Original Assignee
Hoechst Schering Agrevo Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4317596A external-priority patent/DE4317596A1/de
Application filed by Hoechst Schering Agrevo Gmbh filed Critical Hoechst Schering Agrevo Gmbh
Priority to PCT/EP1994/001671 priority Critical patent/WO1994028146A2/fr
Priority to EP94916985A priority patent/EP0701617A1/fr
Priority to US08/553,436 priority patent/US5866790A/en
Publication of WO1994028146A2 publication Critical patent/WO1994028146A2/fr
Publication of WO1994028146A3 publication Critical patent/WO1994028146A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

Definitions

  • the present invention relates to DNA sequences and plasmids, containing these DNA sequences, which by integration into the genome of a sugar-beet plant, changes the sugar metabolism of the plant to be changed.
  • the invention also relates to transgenic plants formed with the help of these sequences.
  • Sucrose is of central importance for the plant and serves many functions. For the long distance transport of photoassimilates and/or energy between various organs in ⁇ lcnts, -"""-rose is almost exclusively used.
  • the sucrose which is transported in a specific heterotrophic organ, determines the growth and the development of this organ.
  • transgenic plants in which the transport away of the sucrose from the exporting leaves is inhibited by expression of an apoplastic invertase, shows a strong reduction in the growth of e.g. roots or tubers in the case of potato plants.
  • sucrose for tobacco plants, the principal importance of sucrose as the central function for the long distance transport of energy carriers within the plant is described (von Schaewen et al, 1990, EMBO J 9: 3033-3044).
  • sucrose and/or the hexoses Besides sucrose and/or the hexoses, glucose and fructose, derived from sucrose, have the property of protection of plants against frost damage at low temperatures.
  • Frost damage is one of the main limiting factors in agricultural productivity in the northern hemisphere. Temperatures below freezing lead to the formation of ice crystals. Since the growing ice crystals consist of pure water, water is abstracted from the cells as the temperature falls.
  • Osmotically active substances include sucrose and/or the two hexoses derived from sucrose.
  • sucrose and/or the two hexoses at low temperatures is desirable in the growing plant. Another situation can exist in the harvested parts of a plant, especially in storage.
  • sucrose thus possesses two especially important functions:
  • sucrose a substance that influences the production of sucrose.
  • the biosynthesis pathways for the formation of sucrose either from the primary photosynthesis products (in the leaf) or by breakdown of starch (in the storage organs e.g. of potatoes) , are known.
  • Each plasmid comprises:
  • c) A non-coding termination sequence that contains the signal for the termination and polyadenylation of the transcript.
  • the coding sequences named under b) are the sequences that code for the large and small subunit of the ADP glucose pyrophosphorylase, for the sucrose phosphate synthase and for the sucrose synthase of sugar beet. 5
  • the large subunit of the ADP-glucose-pyrophosphorylase has the following nucleotide sequence (Seq. ID No. 1) :
  • ATC ATT TTG AAG AAC GCA ACC ATA CAA GAC GGT CTT GTG ATT TAG 1773 lie lie Leu Lys Asn Ala Thr lie Gin Asp Gly Leu Val lie End 510 515 520
  • the small subunit of the ADP-glucose-pyrophosphala ⁇ e has the following nucleotide sequence (Seq. ID No. 2) :
  • GG ATA ACT GTG CCA TCA ACC TCC TCA AAG AAC CTC CAA AAT AGC 0044 lie Thr Val Pro Ser Thr Ser Ser Lys Asn Leu Gin Asn Ser
  • ATA TAT GTT CTT ACA CAA TTC AAT TCT GCT TCT CTG AAT CGT CAT 0404 lie Tyr Val Leu Thr Gin Phe Asn Ser Ala Ser Leu Asn Arg His 115 120 125
  • GAG AAA CCG AAA
  • GGA GAA CAA TTG 0764 Gly Arg lie lie Glu Phe Ala Glu Lys Pro Lys Gly Glu Gin Leu 235 240 245
  • Gly Ser Val Pro lie Gly lie Gly Asn Ala Arg lie Gly Asp Asp 430 435 440
  • Val Lys lie lie Asn Ser Asp Asn Val Gin Glu Ala Ala Arg Glu 445 450 455
  • sucrose phosphate - synthase has the following nucleotide sequence (Seq. ID No. 3) :
  • Trp lie Asn Ser Tyr Leu Glu Ala lie Leu Asp Val Gly Pro
  • AGA ATT TGG AAT TTG GCT CGT CAG AAG AAG CAG CTT GAG AAT GAA 0314 Arg lie Trp Asn Leu Ala Arg Gin Lys Lys Gin Leu Glu Asn Glu
  • GTC CTT GAT AAT GGT CTT CTT GTG GAT CCT CAT GAG CAG CAG TCT 1889 Val Leu Asp Asn Gly Leu Leu Val Asp Pro His Glu Gin Gin Ser
  • AAA CTC TCC AAA GCT TAA TCAGATATCT GCTGCTTTCT TTTGGGTAAG 3197 Lys Leu Ser Lys Ala End
  • sucrose-synthase has the following nucleotide sequence (Seq. ID No. 4) :
  • GGT GAA CTC TAT CGC TAC ATT TGT GAC AAA GGA GGT ATT TTT GCG 1844 Gly Glu Leu Tyr Arg Tyr He Cys Asp Lys Gly Gly He Phe Ala 600 605 610
  • TTA CTA AGG ATC AAA GAA AGA TAT ACC TGG CAA AAG TAT TCT GAA 2114 Leu Leu Arg He Lys Glu Arg Tyr ⁇ _ ⁇ i Trp Gin Lys Tyr Ser Glu 690 695 700
  • sequences can also be combined together in a suitable plasmid which leads to a combination of the individual characteristics, conditioned by the expression of the protein.
  • the promoter should ensure that the foreign gene is expressed in the plant.
  • the promoter can be so chosen that the expression occurs only in specified tissues, at a determined time point in the plant's development or at a time point determined by outside influences.
  • Suitable promoters are e.g. the promoter of the 35S RNA of the cauliflower mosaic virus, the patatin promoter B33 (Rocha-Sosa et al. (1989) EMBO J 8: 23-29) or a promoter that ensures an expression only in photosynthetically active tissues.
  • Other promoters can be used which ensure an expression only in specified organs, such as the root, tuber, seed, stem or specified cell types such as mesophyllic, epidermal or transport cells.
  • the coding sequences described herein contain the information for the formation of an RNA for the large subunit of the ADP-glucose-pyrophosphorylase and the sucrose-phosphate-synthase (SPS) and a part of the information for formation of the small subunit of the ADP-glucose-pyrophosphorylase as well as the sucrose-synthase, that are suitable for the formation of anti-sense RNA to the corresponding genes. Whether a translatable mRNA or an anti-sense nucleic acid is formed, depends on the orientation of the coding sequence in relation to the promoter.
  • SPS sucrose-phosphate-synthase
  • the coding sequence for the large and small subunit of the ADP-glucose-pyrophosphorylase, the sucrose phosphate synthase and the sucrose synthase can be one of those described in this invention or can be one that is derived by modifications of the sequences described above. Thereby especially modifications of the sequences can be considered which lead to by-passing of the plant's own regulation mechanisms. Modifications to the DNA sequences of the invention can be by known methods, such as e.g. base exchange or targeted or non-targeted mutagenesis. The so-formed derivatives of the DNA sequences of the invention are also within the scope of the invention. With plasmids, which contain one or more of the DNA sequences of the invention, sugar beet can be transformed with the object of raising and/or reducing the enzyme activity and/or the change of the sucrose concentration.
  • cloning vectors which contain a replication signal for E . coli and a marker, which allows a selection of the transformed cells.
  • T-DNA for the transformation of plants cells has been intensively researched and is well described in EP 120 516; Hoekama, In: The Binary Plant Vector System, Offset-drukkerij Kanters B.V. Alblasserda , (1985) , Chapter V; Fraley, et al., Crit. Rev. Plant Sci., 4:1-46 and An et al.
  • the transformed cells grow within the plants in the usual manner (see also McCormick et al. (1986) Plant Cell Reports 5: 81-84) . These plants can be grown normally and crossed with plants, that possess the same transformed genes or different. The resulting hybrid individuals have the corresponding phenotypical properties.
  • Bacterial strains The E . coli strain BMH71-18 (Messing et a_l. , Proc. Natl. Acad. Sci. USA (1977), 24, 6342-6346) or TBl was used for the pUC and M13 mP vectors.
  • TBl is a recombi ⁇ ant-negative, tetracycline- resistant derivative of strain JM101 (Yanisch-Perron et al. , Gene (1985) , 33, 103-119) .
  • the genotype of the TBl strain is (Bart Barrel, personal communication) : F'(traD36, proAB, lad, lacZ ⁇ M15) , ⁇ (lac, pro) , SupE, this, recA, Sri: :TnlO (TcR) .
  • the transformation of the plasmids into the potato plants was carried out using Agrobacterium tumefaciens strain LBA4404 (Bevan, (1984), Nucl. Acids Res. .12., 8711-8720) .
  • the insertion of the DNA into the Agrobacterium was effected by direct transformation in accordance with the method of Holsters et al., (1978) (Mol Gene Genet 163: 181-187) .
  • the plasmid DNA of the transformed Agrobacterium was isolated in accordance with the method of Birnboim and Doly (1979) (Nucl Acids Res 7: 1513-1523) and was analysed by gel electrophoresis after suitable restriction cleavage.
  • sucrose phosphate-synthase activity was determined according to the method of Siegel and Stitt (1990, Plant Science 66: 205-210) in a two stage analysis.
  • 50mM HEPES/KOH pH 7.4
  • 5mM magnesium chloride 5mM fructose-6-phosphate, 25mM glucose-6-phosphate and 6mM uridine-5'-diphosphoglucose
  • 20 ⁇ l of probe was added and incubated for 10 minutes at 25°C. It was heated for 3 minutes at 95°C, to complete the reaction.
  • RNA was isolated according to the method of Logemann et al (1987, Anal Bioche 163, 16-20) . Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an Xhol recognising position and for synthesis of the first cDNA strand methylated cytidine nucleotide was inserted.
  • RNA was isolated according to the method of Logemann et al (1987, Anal Bioche 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 2b, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an Xhol recognising position and for synthesis of the first cDNA strand methylated cytidine nucleotide was inserted.
  • RNA was isolated according to the method of Logemann et al (1987, Anal Bioche 163, 16-20) . Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an Xhol recognising position and for synthesis of the first cDNA strand methylated cytidine nucleotide was inserted.
  • nucleotide sequences of the insertions obtained from Examples 1 -3 were determined by standard methods by means of the dideoxy method (Sanger et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467) .
  • the nucleotide sequences and the amino acid sequences derived therefrom are given in the sequence protocols Seq. ID No. 1-4.
  • PROPERTIES ADP-glucose-pyrophosphorylase, large subunit
  • PROPERTIES ADP-glucose-pyrophosphorylase, small subunit SEQ ID NO: 3

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des séquences d'ADN et des plasmides qui modifient la concentration de sucrose lorsqu'ils sont incorporés dans un génome de la betterave, ainsi que des plantes transgéniques présentant des modifications de la concentration de sucre, obtenues par l'introduction des séquences d'ADN selon l'invention.
PCT/EP1994/001671 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee WO1994028146A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/EP1994/001671 WO1994028146A2 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee
EP94916985A EP0701617A1 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee
US08/553,436 US5866790A (en) 1993-05-24 1994-05-20 DNA sequences and plasmids for the preparation of sugar beet with changed sucrose concentration

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DEP4317596.1 1993-05-24
DE4317596A DE4317596A1 (de) 1993-05-24 1993-05-24 DNA-Sequenzen und Plasmide zur Herstellung von Zuckerrüben mit veränderter Saccharose-Konzentration
PCT/EP1994/001671 WO1994028146A2 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee

Publications (2)

Publication Number Publication Date
WO1994028146A2 true WO1994028146A2 (fr) 1994-12-08
WO1994028146A3 WO1994028146A3 (fr) 1995-01-26

Family

ID=25926255

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1994/001671 WO1994028146A2 (fr) 1993-05-24 1994-05-20 Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee

Country Status (1)

Country Link
WO (1) WO1994028146A2 (fr)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034660A1 (fr) * 1994-06-16 1995-12-21 Advanced Technologies (Cambridge) Limited Modification de la teneur en amidon de plantes
WO1996021738A1 (fr) * 1995-01-15 1996-07-18 Calgene, Inc. Modification de solides solubles utilisant des sequences codant la sucrose phosphate synthase
WO1997032027A1 (fr) * 1996-02-29 1997-09-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Berlin Regulon de la betterave sucriere, specifique du tissu radiculaire de reserve
WO1998003637A1 (fr) * 1996-07-19 1998-01-29 Arch Development Corporation Compositions a base de saccharose synthase bacterienne et techniques d'utilisation
WO1999023234A1 (fr) * 1997-10-30 1999-05-14 Mogen International N.V. Inhibition de la remobilisation des composes stockes avant et apres recolte
US5981852A (en) * 1990-07-20 1999-11-09 Calgene Llc Modification of sucrose phosphate synthase in plants
WO2000022092A2 (fr) * 1998-10-13 2000-04-20 Genesis Research And Development Corporation Limited Materiels et procedes de modification de polysaccharides de parois cellulaires vegetales
US6124528A (en) * 1995-01-15 2000-09-26 Calgene Llc Modification of soluble solids in fruit using sucrose phosphate synthase encoding sequence
AU726010B2 (en) * 1995-10-27 2000-10-26 Calgene, Inc. Modification of soluble solids using sucrose phosphate synthase encoding sequence
WO2004083440A1 (fr) * 2003-03-20 2004-09-30 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Expression de ppase modifiee dans la betterave a sucre
WO2005075649A1 (fr) * 2004-02-05 2005-08-18 Universidad Publica De Navarra Procede de production de saccharose synthase recombinante, son utilisation dans la fabrication de kits de determination de saccharose, production de adpglucose et obtention de plantes transgeniques dont les feuilles et les organes de reserve accumulent une forte teneur en adpglucose et amidon
US7012171B2 (en) 1989-12-21 2006-03-14 Advanced Technologies Cambridge Limited Modification of plant metabolism
WO2007018770A2 (fr) * 2005-07-22 2007-02-15 Syngenta Participations Ag Gene de chlamydomonas glucan dikinase, enzyme et amidon modifie, utilisations, procedes de production associes
US7247769B2 (en) 1998-07-31 2007-07-24 Bayer Cropscience Gmbh Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
WO2015030667A1 (fr) * 2013-08-29 2015-03-05 Sveriges Stärkelseproducenter, Förening UPA Plante transgénique
WO2015113118A1 (fr) * 2014-01-29 2015-08-06 The University Of Queensland Promoteur de rendement pour l'augmentation de saccharose et de dérivés de saccharose dans des plantes

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438904A1 (fr) * 1989-12-21 1991-07-31 Advanced Technologies (Cambridge) Limited Modification du métabolisme végétal
EP0455316A2 (fr) * 1990-04-20 1991-11-06 Institut Für Genbiologische Forschung Berlin Gmbh Plasmides contenant des séquences d'ADN entraînant la modification de la concentration et de la composition en hydrates de carbones et en protéines dans les plantes, ainsi que des cellules de plantes et des plantes contenant ces plasmides
WO1991019806A1 (fr) * 1990-06-18 1991-12-26 Monsanto Company Plantes a teneur en amidon augmentee
EP0466995A2 (fr) * 1990-07-20 1992-01-22 Roussel Uclaf Saccharose phosphate Synthétase (SPS), son procédé de préparation, son ADNc et utilisation de l'ADNc pour modifier l'expression de la SPS dans les cellules végétales
WO1992016631A1 (fr) * 1991-03-18 1992-10-01 Roussel-Uclaf SYNTHASE DE PHOSPHATE DE SUCROSE (SPS), PROCEDE DE PREPARATION, SON ADNc ET UTILISATION DE L'ADNc POUR MODIFIER L'EXPRESSION DE SPS DANS LES CELLULES VEGETALES
EP0530978A2 (fr) * 1991-08-08 1993-03-10 Advanced Technologies (Cambridge) Limited Modification de l'accumulation de saccharose
WO1993009237A1 (fr) * 1991-11-05 1993-05-13 Sandoz Ltd. Mais tres sucre et ameliore

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438904A1 (fr) * 1989-12-21 1991-07-31 Advanced Technologies (Cambridge) Limited Modification du métabolisme végétal
EP0455316A2 (fr) * 1990-04-20 1991-11-06 Institut Für Genbiologische Forschung Berlin Gmbh Plasmides contenant des séquences d'ADN entraînant la modification de la concentration et de la composition en hydrates de carbones et en protéines dans les plantes, ainsi que des cellules de plantes et des plantes contenant ces plasmides
WO1991019806A1 (fr) * 1990-06-18 1991-12-26 Monsanto Company Plantes a teneur en amidon augmentee
EP0466995A2 (fr) * 1990-07-20 1992-01-22 Roussel Uclaf Saccharose phosphate Synthétase (SPS), son procédé de préparation, son ADNc et utilisation de l'ADNc pour modifier l'expression de la SPS dans les cellules végétales
WO1992016631A1 (fr) * 1991-03-18 1992-10-01 Roussel-Uclaf SYNTHASE DE PHOSPHATE DE SUCROSE (SPS), PROCEDE DE PREPARATION, SON ADNc ET UTILISATION DE L'ADNc POUR MODIFIER L'EXPRESSION DE SPS DANS LES CELLULES VEGETALES
EP0530978A2 (fr) * 1991-08-08 1993-03-10 Advanced Technologies (Cambridge) Limited Modification de l'accumulation de saccharose
WO1993009237A1 (fr) * 1991-11-05 1993-05-13 Sandoz Ltd. Mais tres sucre et ameliore

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIOLOGICAL ABSTRACTS, vol. 85 Philadelphia, PA, US; abstract no. 72586, FIEUW, S., ET AL. 'Sucrose synthase and sucrose phosphate synthase in sugar beet plants Beta-vulgaris -ssp-altissima' & J. PLANT PHYSIOL., vol.131, no.1-2, 1987 pages 153 - 162 *
BIOLOGICAL ABSTRACTS, vol. 95 Philadelphia, PA, US; abstract no. 79002, SAKALO, V.D., ET AL. 'Characterization of molecular forms of sucrose synthase from beet roots' & FIZIOL RAST (MOSC), vol.39, no.2, 1992 pages 290 - 299 *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US DN BR35:113623 MURAKAMI, T. 'Histochemical loclaization of UDPG ADPG-pyrophosphorylase and phosphorylase in young petiole of sugar beet Beta-vulgaris L.' & J. HISTOCHEM. CYTOCHEM., vol.36, no.7A, 1988 page 927 *
EMBL SEQUENCE DATABASE ACC. NO. S54379 Sucrose phosphate synthase release 35; 31-MAR-1993 *
GENE, vol.60, 1987, AMSTERDAM NL pages 47 - 56 SALANOUBAT, M., ET AL. 'Molecular cloning and sequencing of sucrose synthase cDNA from potato (Solanum tuberosum L.): preliminary characterization of sucrose synthase mRNA distribution' *

Cited By (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7012171B2 (en) 1989-12-21 2006-03-14 Advanced Technologies Cambridge Limited Modification of plant metabolism
US5981852A (en) * 1990-07-20 1999-11-09 Calgene Llc Modification of sucrose phosphate synthase in plants
US6096945A (en) * 1994-06-16 2000-08-01 Advanced Technologies (Cambridge) Limited Modification of starch content in plants
US6486383B1 (en) 1994-06-16 2002-11-26 Advanced Technologies (Cambridge) Limited Modification of starch content in plants
WO1995034660A1 (fr) * 1994-06-16 1995-12-21 Advanced Technologies (Cambridge) Limited Modification de la teneur en amidon de plantes
WO1996021738A1 (fr) * 1995-01-15 1996-07-18 Calgene, Inc. Modification de solides solubles utilisant des sequences codant la sucrose phosphate synthase
WO1997015678A2 (fr) * 1995-01-15 1997-05-01 Calgene, Inc. Modification de solides solubles a l'aide d'une sequence codant la phosphate synthase de saccharose
WO1997015678A3 (fr) * 1995-01-15 1997-10-16 Calgene Inc Modification de solides solubles a l'aide d'une sequence codant la phosphate synthase de saccharose
US5914446A (en) * 1995-01-15 1999-06-22 Calgene, Llc Soluble solids modification using sucrose phosphate synthase encoding sequences
US6124528A (en) * 1995-01-15 2000-09-26 Calgene Llc Modification of soluble solids in fruit using sucrose phosphate synthase encoding sequence
AU726010B2 (en) * 1995-10-27 2000-10-26 Calgene, Inc. Modification of soluble solids using sucrose phosphate synthase encoding sequence
WO1997032027A1 (fr) * 1996-02-29 1997-09-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Berlin Regulon de la betterave sucriere, specifique du tissu radiculaire de reserve
US6248936B1 (en) 1996-02-29 2001-06-19 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Sugarbeet storage-root-tissue-specific regulon
US6682918B1 (en) 1996-07-19 2004-01-27 Arch Development Corporation Bacterial sucrose synthase compositions and methods of use
WO1998003637A1 (fr) * 1996-07-19 1998-01-29 Arch Development Corporation Compositions a base de saccharose synthase bacterienne et techniques d'utilisation
US6559364B1 (en) 1997-10-30 2003-05-06 Mogen International N.V. Pre- and postharvest inhibition of remobilisation of storage compounds
WO1999023234A1 (fr) * 1997-10-30 1999-05-14 Mogen International N.V. Inhibition de la remobilisation des composes stockes avant et apres recolte
US7247769B2 (en) 1998-07-31 2007-07-24 Bayer Cropscience Gmbh Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
US7385104B2 (en) 1998-07-31 2008-06-10 Bayer Cropscience Ag Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
WO2000022092A2 (fr) * 1998-10-13 2000-04-20 Genesis Research And Development Corporation Limited Materiels et procedes de modification de polysaccharides de parois cellulaires vegetales
AU777500B2 (en) * 1998-10-13 2004-10-21 Arborgen Llc Materials and methods for the modification of plant cell wall polysaccharides
WO2000022092A3 (fr) * 1998-10-13 2000-07-13 Genesis Res & Dev Corp Ltd Materiels et procedes de modification de polysaccharides de parois cellulaires vegetales
WO2004083440A1 (fr) * 2003-03-20 2004-09-30 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Expression de ppase modifiee dans la betterave a sucre
WO2005075649A1 (fr) * 2004-02-05 2005-08-18 Universidad Publica De Navarra Procede de production de saccharose synthase recombinante, son utilisation dans la fabrication de kits de determination de saccharose, production de adpglucose et obtention de plantes transgeniques dont les feuilles et les organes de reserve accumulent une forte teneur en adpglucose et amidon
ES2245867A1 (es) * 2004-02-05 2006-01-16 Universidad Publica De Navarra Procedimiento produccion sacarosa sintasa recombinante a partir de escherichia coli y uso en fabricacion de kits de determinacion de sacarosa, produccion de azucares-nucleotidos y obtencion de plantas transgenicas con alto contenido de almidon y alto balance amilosa/amilopectina.
US8168856B2 (en) 2004-02-05 2012-05-01 Universidad Publica De Navarra Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of adpglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of adpglucose and starch
CN1984994B (zh) * 2004-02-05 2013-11-20 纳瓦拉公立大学 重组蔗糖合酶的生产方法、及其在生产蔗糖测定试剂盒中的应用、生产腺苷二磷酸葡糖的方法和获得具有积累了高浓度的腺苷二磷酸葡萄糖和淀粉的叶片和储藏器官的转基因植物的方法
US8841514B2 (en) 2004-02-05 2014-09-23 Universidad Publica De Navarra Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of adpglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of adpglucose and starch
USRE46642E1 (en) 2004-02-05 2017-12-19 Universidad Publica De Navarra Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of ADPglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of ADPglucose and starch
WO2007018770A2 (fr) * 2005-07-22 2007-02-15 Syngenta Participations Ag Gene de chlamydomonas glucan dikinase, enzyme et amidon modifie, utilisations, procedes de production associes
WO2007018770A3 (fr) * 2005-07-22 2007-12-21 Syngenta Participations Ag Gene de chlamydomonas glucan dikinase, enzyme et amidon modifie, utilisations, procedes de production associes
US8076534B2 (en) 2005-07-22 2011-12-13 Syngenta Participations Ag Chlamydomonas glucan dikinase gene, enzyme and modified starch, uses, methods for production thereof
WO2015030667A1 (fr) * 2013-08-29 2015-03-05 Sveriges Stärkelseproducenter, Förening UPA Plante transgénique
WO2015113118A1 (fr) * 2014-01-29 2015-08-06 The University Of Queensland Promoteur de rendement pour l'augmentation de saccharose et de dérivés de saccharose dans des plantes

Also Published As

Publication number Publication date
WO1994028146A3 (fr) 1995-01-26

Similar Documents

Publication Publication Date Title
US6723898B2 (en) Methods for the preparation of plants with changed sucrose concentration by transformation with a sucrose phosphate synthase nucleic acid
EP0812917B1 (fr) Sequences promotrices inductibles a froid
US7153674B2 (en) Nucleic acid molecules encoding enzymes having fructosyl polymerase activity
AU645302B2 (en) Plasmids containing DNA sequences that bring about changes in the carbohydrate and protein concentration and the carbohydrate and protein composition in plants, and plant cells and plants containing those plasmids
WO1994028146A2 (fr) Sequences d'adn et plasmides destines a la production d'une betterave a concentration de sucre modifiee
WO1994004692A1 (fr) Sequences d'adn induisant la formation de polyfructanes (levanes), plasmides contenant ces sequences et procede de preparation de plantes transgeniques
DE19749122A1 (de) Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen
HU218048B (hu) A habitusban és hozamban megváltoztatott transzgenikus növények előállítása plazmidok segítségével
JP3431177B2 (ja) 習性及び収量において変更されたトランスジェニック植物を作製するプラスミド
HUT67084A (en) Expression cassette and plasmids for a guard cell specific expression and their use for the introduction of transgenic plant cells and plants
GB2360521A (en) Genetic modification of starch in plants
US5866790A (en) DNA sequences and plasmids for the preparation of sugar beet with changed sucrose concentration
EP0909314A4 (fr) Plantes detectant les sucres et leurs utilisations
RU2145636C1 (ru) Способ индуцирования образования цветков у растений
JPH0889250A (ja) アロエのリンゴ酸酵素遺伝子
AU4441102A (en) Nucleic acid molecules from artichoke (cynara scolymus) encoding enzymes having fructosyl polymerase activity

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA HU JP RU UA US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): CA HU JP RU UA US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1994916985

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 08553436

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1994916985

Country of ref document: EP

NENP Non-entry into the national phase in:

Ref country code: CA

WWR Wipo information: refused in national office

Ref document number: 1994916985

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1994916985

Country of ref document: EP