WO1994025594A3 - Cloning, expression and vaccine composition corresponding to a peptide antigen of toxoplasma gondii - Google Patents

Cloning, expression and vaccine composition corresponding to a peptide antigen of toxoplasma gondii Download PDF

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Publication number
WO1994025594A3
WO1994025594A3 PCT/EP1994/001298 EP9401298W WO9425594A3 WO 1994025594 A3 WO1994025594 A3 WO 1994025594A3 EP 9401298 W EP9401298 W EP 9401298W WO 9425594 A3 WO9425594 A3 WO 9425594A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
cloning
vaccine composition
peptide antigen
toxoplasma gondii
Prior art date
Application number
PCT/EP1994/001298
Other languages
French (fr)
Other versions
WO1994025594A2 (en
Inventor
Ralph Biemans
Alex Bollen
Paul Jacobs
Original Assignee
Smithkline Beecham Biolog
Ralph Biemans
Alex Bollen
Paul Jacobs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939308969A external-priority patent/GB9308969D0/en
Priority claimed from GB939313797A external-priority patent/GB9313797D0/en
Application filed by Smithkline Beecham Biolog, Ralph Biemans, Alex Bollen, Paul Jacobs filed Critical Smithkline Beecham Biolog
Priority to JP6523864A priority Critical patent/JPH08512200A/en
Priority to EP94915540A priority patent/EP0695356A1/en
Publication of WO1994025594A2 publication Critical patent/WO1994025594A2/en
Publication of WO1994025594A3 publication Critical patent/WO1994025594A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/45—Toxoplasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The invention provides a polypeptide corresponding to residues 197 - 216 of the 54 KDa antigen of T. gondii, having the amino acid sequence TDPGDVVIEELFNRIPETSV. Also within the scope of the invention is a method of producing the 54 KD antigen of T. gondii or the polypeptide corresponding to residues 197 - 216 thereof by expression from a host cell, for example from a host cell comprising a vaccinia virus or from a CHO cell.
PCT/EP1994/001298 1993-04-30 1994-04-26 Cloning, expression and vaccine composition corresponding to a peptide antigen of toxoplasma gondii WO1994025594A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP6523864A JPH08512200A (en) 1993-04-30 1994-04-26 Vaccine composition for cloning, expression and peptide antigen of Toxoplasma gondii
EP94915540A EP0695356A1 (en) 1993-04-30 1994-04-26 Cloning, expression and vaccine composition corresponding to a peptide antigen of toxoplasma gondii

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB939308969A GB9308969D0 (en) 1993-04-30 1993-04-30 Novel compounds
GB939313797A GB9313797D0 (en) 1993-07-03 1993-07-03 Novel compounds
GB9313797.4 1993-07-03
GB9308969.6 1993-07-03

Publications (2)

Publication Number Publication Date
WO1994025594A2 WO1994025594A2 (en) 1994-11-10
WO1994025594A3 true WO1994025594A3 (en) 1995-06-01

Family

ID=26302834

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1994/001298 WO1994025594A2 (en) 1993-04-30 1994-04-26 Cloning, expression and vaccine composition corresponding to a peptide antigen of toxoplasma gondii

Country Status (3)

Country Link
EP (1) EP0695356A1 (en)
JP (1) JPH08512200A (en)
WO (1) WO1994025594A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011366A1 (en) * 1990-12-20 1992-07-09 Smithkline Beecham Biologicals (S.A.) Cloning and expression of a protein antigen of toxoplasma gondii
EP0601979A2 (en) * 1992-12-10 1994-06-15 Fondazione Andrea Cesalpino Exogenous antigens bound to hepatitis B virus envelope antigen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011366A1 (en) * 1990-12-20 1992-07-09 Smithkline Beecham Biologicals (S.A.) Cloning and expression of a protein antigen of toxoplasma gondii
EP0601979A2 (en) * 1992-12-10 1994-06-15 Fondazione Andrea Cesalpino Exogenous antigens bound to hepatitis B virus envelope antigen

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J.B. ROTHBARD AND W.R. TAYLOR: "A sequence pattern common to T cell epitopes", EMBO J., vol. 7, no. 1, OXFORD UNIVERSITY PRESS,GB;, pages 93 - 100 *
K.J. COLLEY ET AL.: "Conversion of a golgi apparatus sialyltransferase to a secretory protein by replacement of the NH2-terminal signal anchor with a signal peptide", J. BIOL. CHEM., vol. 264, no. 30, 25 October 1989 (1989-10-25), AM. SOC. BIOCHEM. MOL.BIOL.,INC.,BALTIMORE,US;, pages 17619 - 17622 *
R. SAAVEDRA AND HÉRION: "Human T-cell clones against Toxoplasma gondii: production of interferon-gamma, interleukin-2, and strain cross-reactivity", PARASITOLOGY RESEARCH, vol. 77, no. 5, SPRINGER VERLAG, BERLIN,BRD, pages 379 - 385 *
T. RUTGERS ET AL.: "Hepatitis B surface antigen as carrier matrix for the repetitive epitope of the circumsporozoite protein of Plasmodium falciparum", BIOTECHNOLOGY, vol. 6, no. 9, NATURE PUBL. CO.,NEW YORK, US, pages 1065 - 1070 *

Also Published As

Publication number Publication date
EP0695356A1 (en) 1996-02-07
WO1994025594A2 (en) 1994-11-10
JPH08512200A (en) 1996-12-24

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