GB2341606A - Glycosylation peptides - Google Patents
Glycosylation peptidesInfo
- Publication number
- GB2341606A GB2341606A GB9930098A GB9930098A GB2341606A GB 2341606 A GB2341606 A GB 2341606A GB 9930098 A GB9930098 A GB 9930098A GB 9930098 A GB9930098 A GB 9930098A GB 2341606 A GB2341606 A GB 2341606A
- Authority
- GB
- United Kingdom
- Prior art keywords
- sequence
- glycosylation
- encodes
- fragment
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000013595 glycosylation Effects 0.000 title abstract 5
- 238000006206 glycosylation reaction Methods 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 239000012634 fragment Substances 0.000 abstract 3
- 108020004511 Recombinant DNA Proteins 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 241000186361 Actinobacteria <class> Species 0.000 abstract 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 229960001230 asparagine Drugs 0.000 abstract 1
- 235000009582 asparagine Nutrition 0.000 abstract 1
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 abstract 1
- 230000002163 immunogen Effects 0.000 abstract 1
- 230000005847 immunogenicity Effects 0.000 abstract 1
- 101150023079 mpt83 gene Proteins 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 abstract 1
- 241001446247 uncultured actinomycete Species 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A recombinant DNA which encodes a polypeptide which is other than a wild-type or native polypeptide and which is glycosylated when expressed in an actinomycete host, said DNA comprising a sequence which encodes the amino acid sequence: PAAPVTTAA (SEQ ID NO. 1) or a fragment or variant thereof; said fragment or variant including at least both the threonine residues and being able to act as a glycosylation motif; and/or a sequence which encodes a glycosylation motif found in the region of the MPT83 protein including asparagine at position 192 in the sequence, such as the sequence: DLTVIGARDDLMVNNAGL (SEQ ID NO. 3) or a fragment thereof. These recombinant DNA are useful in the production of vaccines, since, they can provide a glycosylation site, recognised in particular by actinomycetes. Glycosylation is preferred as this may improve immunogenicity and stability of an immunogenic protein.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9714242.6A GB9714242D0 (en) | 1997-07-07 | 1997-07-07 | Glycosylation peptides |
| PCT/GB1998/001989 WO1999002706A1 (en) | 1997-07-07 | 1998-07-06 | Glycosylation peptides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB9930098D0 GB9930098D0 (en) | 2000-02-09 |
| GB2341606A true GB2341606A (en) | 2000-03-22 |
Family
ID=10815445
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GBGB9714242.6A Ceased GB9714242D0 (en) | 1997-07-07 | 1997-07-07 | Glycosylation peptides |
| GB9930098A Withdrawn GB2341606A (en) | 1997-07-07 | 1998-07-06 | Glycosylation peptides |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GBGB9714242.6A Ceased GB9714242D0 (en) | 1997-07-07 | 1997-07-07 | Glycosylation peptides |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU8231998A (en) |
| GB (2) | GB9714242D0 (en) |
| WO (1) | WO1999002706A1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992014823A1 (en) * | 1991-02-14 | 1992-09-03 | N.V. Innogenetics S.A. | Mycobacterium polypeptides and nucleic acids encoding them for diagnosis and control of tuberculosis |
| WO1994005780A1 (en) * | 1992-08-28 | 1994-03-17 | The Public Health Research Institute Of The City Of New York, Inc. | Fusion glycoproteins |
| WO1997008322A1 (en) * | 1995-08-25 | 1997-03-06 | The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Mpt83 gene from mycobacterium tuberculosis |
-
1997
- 1997-07-07 GB GBGB9714242.6A patent/GB9714242D0/en not_active Ceased
-
1998
- 1998-07-06 AU AU82319/98A patent/AU8231998A/en not_active Abandoned
- 1998-07-06 GB GB9930098A patent/GB2341606A/en not_active Withdrawn
- 1998-07-06 WO PCT/GB1998/001989 patent/WO1999002706A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992014823A1 (en) * | 1991-02-14 | 1992-09-03 | N.V. Innogenetics S.A. | Mycobacterium polypeptides and nucleic acids encoding them for diagnosis and control of tuberculosis |
| WO1994005780A1 (en) * | 1992-08-28 | 1994-03-17 | The Public Health Research Institute Of The City Of New York, Inc. | Fusion glycoproteins |
| WO1997008322A1 (en) * | 1995-08-25 | 1997-03-06 | The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Mpt83 gene from mycobacterium tuberculosis |
Non-Patent Citations (1)
| Title |
|---|
| Pharmaceutical Research Vol. 10, No. 9, 1 September 1993, pages 1268-1273, Powell et al. * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU8231998A (en) | 1999-02-08 |
| WO1999002706A1 (en) | 1999-01-21 |
| GB9714242D0 (en) | 1997-09-10 |
| GB9930098D0 (en) | 2000-02-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |