WO1993014768A1 - Procedes et compositions supprimant l'activite biologique d'une proteine de liaison d'acide nucleique intracellulaire dans une cellule, et procedes et compositions utilises pour reguler une fonction genique - Google Patents

Procedes et compositions supprimant l'activite biologique d'une proteine de liaison d'acide nucleique intracellulaire dans une cellule, et procedes et compositions utilises pour reguler une fonction genique Download PDF

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Publication number
WO1993014768A1
WO1993014768A1 PCT/US1993/000440 US9300440W WO9314768A1 WO 1993014768 A1 WO1993014768 A1 WO 1993014768A1 US 9300440 W US9300440 W US 9300440W WO 9314768 A1 WO9314768 A1 WO 9314768A1
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WO
WIPO (PCT)
Prior art keywords
protein
biological activity
cell
dna
rna
Prior art date
Application number
PCT/US1993/000440
Other languages
English (en)
Inventor
Dan Liebermann
Barbara Hoffman-Liebermann
Original Assignee
The Trustees Of The University Of Pennsylvania
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of The University Of Pennsylvania filed Critical The Trustees Of The University Of Pennsylvania
Publication of WO1993014768A1 publication Critical patent/WO1993014768A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/13—Decoys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Definitions

  • RNA transcripts in the cell nucleus are also important in eukaryotic gene control.
  • chromosomal segments are amplified or rearranged so as to effect gene expression.
  • Intracellular immunization the genetic alteration of cells that is mediated by gene transfer and leads to the inhibition of viral replication has been termed "intracellular immunization" .
  • Intracellular immunization is perceived as being achievable by stable introduction of DNA into cells whose continuous expression results in the inhibition of an essential viral gene.
  • antisense RNA see eintraub et al. Trends Genet. (1985) 2:22-25; Green et al, Annu. Rev. Bioche . (1986), .55:569-597; van der Krol et al. Bio. Technigues (1988), 6 .
  • DNA-binding proteins exhibit a limited number of structural designs.
  • Three general structural designs have been found in DNA-binding proteins: the helix-turn-helix, the zinc-finger, and the amphipathic helix motifs.
  • Helix-turn-helix proteins possess a remarkably conserved region encoding about 60 amino acids, called the homeobox, which is conserved well enough to be easily recognized in genes from frogs to mammals. These homeobox proteins are believed to contact specific DNA sequences.
  • Zinc-finger proteins and amphipathic helical proteins, including leucine-zipper and helix-loop-helix proteins are also known to be regulatory proteins.
  • compositions of the present invention have broad applications for both unicellular and multicellular organisms, including, in particular, therapeutic potential for the latter.
  • the present invention can be applied to yeast and bacterial systems as well as to either plants or animals.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Procédé de suppression de l'activité biologique d'une protéine de liaison d'acide nucléique intracellulaire dans une cellule, et qui consiste à exposer cette protéine à une concentration intracellulaire d'un ADN ou d'un ARN simple-brin (sb), ou d'un ADN ou d'un ARN double-brin (db) présentant un motif de reconnaissance par rapport à la protéine, lequel est suffisant pour effectuer la liaison de la protéine à l'ADN (sb), l'ARN (sb), ADN (db) ou l'ARN (db), ce qui permet de bloquer l'activité de la protéine intracellulaire.
PCT/US1993/000440 1992-01-27 1993-01-27 Procedes et compositions supprimant l'activite biologique d'une proteine de liaison d'acide nucleique intracellulaire dans une cellule, et procedes et compositions utilises pour reguler une fonction genique WO1993014768A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82607092A 1992-01-27 1992-01-27
US826,070 1992-01-27

Publications (1)

Publication Number Publication Date
WO1993014768A1 true WO1993014768A1 (fr) 1993-08-05

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Application Number Title Priority Date Filing Date
PCT/US1993/000440 WO1993014768A1 (fr) 1992-01-27 1993-01-27 Procedes et compositions supprimant l'activite biologique d'une proteine de liaison d'acide nucleique intracellulaire dans une cellule, et procedes et compositions utilises pour reguler une fonction genique

Country Status (2)

Country Link
AU (1) AU3584993A (fr)
WO (1) WO1993014768A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0733370A1 (fr) * 1993-10-29 1996-09-25 Shunichi Shiozawa Inhibiteur antagoniste de la croissance des cellules mesenchymateuses
EP0732929A1 (fr) * 1993-10-29 1996-09-25 Victor J. Dzau Utilisation therapeutique de leurres d'elements cis in vivo
EP0776335A1 (fr) * 1994-08-18 1997-06-04 Ariad Gene Therapeutics, Inc. Elimination modulable d'expression genique, de fonction de produit genique, et de cellules hotes obtenues par genie genetique
EP0805160A1 (fr) * 1996-05-01 1997-11-05 FUJIREBIO Inc. Polypeptide lié à l'aide nucléique, procédé de préparation d'un polypeptide lié à l'acide nucléique et immunoessai utilisant le polypeptide
US5859226A (en) * 1993-07-29 1999-01-12 Regents Of The University Of California, The Polynucleotide decoys that inhibit MHC-II expression and uses thereof
WO2002018607A2 (fr) * 2000-08-30 2002-03-07 North Carolina State University Plantes transgeniques contenant des leurres moleculaires qui modifient la teneur en proteines
US6410721B1 (en) 1993-07-29 2002-06-25 The Regents Of The University Of California Polynucleotide decoys that inhibit MHC-II expression and uses thereof
US7045363B2 (en) * 1996-05-01 2006-05-16 Fujirebio Inc. Nucleic acid-bound polypeptide method of producing nucleic acid-bound polypeptide and immunoassay using the polypeptide
US7615538B2 (en) 1993-10-29 2009-11-10 Shunichi Shiozawa Method for therapy of rheumatoid arthritis

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF IMMUNOLOGY, Volume 140, No. 7, issued 01 April 1988, A. HAREL-BELLAN et al., "Specific Inhibition of C-Myc Protein Biosynthesis Using an Antisense Synthetic Deoxy-Oligonucleotide in Human T Lymphocytes", pages 2431-2435. *
PROCEEDINGS NATIONAL ACADEMY OF SCIENCES, Volume 83, issued July 1986, J.T. HOLT et al., "Inducible Production of C-Fos Antisense RNA Inhibits 3T3 Cell Proliferation", pages 4794-4798. *
PROCEEDINGS NATIONAL ACADEMY OF SCIENCES, Volume 86, issued May 1989, G. ANFOSSI et al., "An Oligomer Complementary to C-Myb-Encoded mRNA Inhibits Proliferation of Human Myeloid Leukemia Cell Lines", pages 3379-3383. *
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, Volume 88, issued May 1991, L. HAN et al., "Inhibition of Moloney Murine Leukemia Virus-Induced Leukemia in Transgenic Mice Expressing Antisense RNA Complementary to the Retroviral Packaging Sequences", pages 4313-4317. *

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5859226A (en) * 1993-07-29 1999-01-12 Regents Of The University Of California, The Polynucleotide decoys that inhibit MHC-II expression and uses thereof
US6410721B1 (en) 1993-07-29 2002-06-25 The Regents Of The University Of California Polynucleotide decoys that inhibit MHC-II expression and uses thereof
US6821956B2 (en) 1993-10-29 2004-11-23 The Brigham And Women's Hospital, Inc. Therapeutic use of cis-element decoys in vivo
EP1350514A3 (fr) * 1993-10-29 2004-07-07 The Brigham And Women's Hospital, Inc. Utilisation thérapeutique de leurres d'élements cis in vivo
US7615538B2 (en) 1993-10-29 2009-11-10 Shunichi Shiozawa Method for therapy of rheumatoid arthritis
EP0733370A1 (fr) * 1993-10-29 1996-09-25 Shunichi Shiozawa Inhibiteur antagoniste de la croissance des cellules mesenchymateuses
EP0733370A4 (fr) * 1993-10-29 1999-12-29 Shunichi Shiozawa Inhibiteur antagoniste de la croissance des cellules mesenchymateuses
EP0732929A4 (fr) * 1993-10-29 2001-11-14 Victor J Dzau Utilisation therapeutique de leurres d'elements cis in vivo
US6774118B1 (en) 1993-10-29 2004-08-10 The Brigham And Women's Hospital, Inc. Therapeutic use of CIS-element decoys in vivo
EP0732929A1 (fr) * 1993-10-29 1996-09-25 Victor J. Dzau Utilisation therapeutique de leurres d'elements cis in vivo
EP1340505A3 (fr) * 1993-10-29 2004-07-14 The Brigham And Women's Hospital, Inc. Utilisation thérapeutique de leurres d'éléments-cis in vivo
EP1340505A2 (fr) * 1993-10-29 2003-09-03 The Brigham And Women's Hospital, Inc. Utilisation thérapeutique de leurres d'éléments-cis in vivo
EP1350514A2 (fr) * 1993-10-29 2003-10-08 The Brigham And Women's Hospital, Inc. Utilisation thérapeutique de leurres d'élements cis in vivo
EP0776335A4 (fr) * 1994-08-18 1999-09-15 Ariad Gene Therapeutics Inc Elimination modulable d'expression genique, de fonction de produit genique, et de cellules hotes obtenues par genie genetique
EP0776335A1 (fr) * 1994-08-18 1997-06-04 Ariad Gene Therapeutics, Inc. Elimination modulable d'expression genique, de fonction de produit genique, et de cellules hotes obtenues par genie genetique
EP0805160A1 (fr) * 1996-05-01 1997-11-05 FUJIREBIO Inc. Polypeptide lié à l'aide nucléique, procédé de préparation d'un polypeptide lié à l'acide nucléique et immunoessai utilisant le polypeptide
US7045363B2 (en) * 1996-05-01 2006-05-16 Fujirebio Inc. Nucleic acid-bound polypeptide method of producing nucleic acid-bound polypeptide and immunoassay using the polypeptide
WO2002018607A3 (fr) * 2000-08-30 2003-02-27 Univ North Carolina State Plantes transgeniques contenant des leurres moleculaires qui modifient la teneur en proteines
WO2002018607A2 (fr) * 2000-08-30 2002-03-07 North Carolina State University Plantes transgeniques contenant des leurres moleculaires qui modifient la teneur en proteines
CN1330753C (zh) * 2000-08-30 2007-08-08 北卡罗莱纳州立大学 含有可改变蛋白含量的分子诱导物的转基因植物

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Publication number Publication date
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