WO1991016631A1 - Analyse immunologique simplifiee en phase solide effectuee a l'aide de billes colorees pour detecter la ciguatoxine ou des polyethers apparentes - Google Patents

Analyse immunologique simplifiee en phase solide effectuee a l'aide de billes colorees pour detecter la ciguatoxine ou des polyethers apparentes Download PDF

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Publication number
WO1991016631A1
WO1991016631A1 PCT/US1991/002703 US9102703W WO9116631A1 WO 1991016631 A1 WO1991016631 A1 WO 1991016631A1 US 9102703 W US9102703 W US 9102703W WO 9116631 A1 WO9116631 A1 WO 9116631A1
Authority
WO
WIPO (PCT)
Prior art keywords
fish
ciguatoxin
eia
spia
detection
Prior art date
Application number
PCT/US1991/002703
Other languages
English (en)
Inventor
Yoshitsugi Hokama
Original Assignee
Hawaii Chemtect International
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hawaii Chemtect International filed Critical Hawaii Chemtect International
Priority to BR919106366A priority Critical patent/BR9106366A/pt
Priority to JP91508651A priority patent/JPH05507554A/ja
Publication of WO1991016631A1 publication Critical patent/WO1991016631A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi

Definitions

  • the invention relates to the use of antibodies for the detection of ciguatoxin in fish.
  • S-EIA simple stick enzyme immuno- assay
  • Monoclonal antibodies The method of Schrier et al., Hybridoma Techniques, Cold Harbor Laboratory, Cold Spring, NY, 1980, incorporated herein by this reference, based on the original report of Kohler et al., "Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity," Nature, 156, 494-497 (1975), incorporated herein by this reference, was used.
  • Hybridoma preparation for Mab-CTX was carried out using purified CTX according to methods of Hokama et al., "Assessment of a Rapid Enzyme Immunoassay Stick Test for the Detection of Ciguatoxin and Related Polyether Toxins in Fish Tissues," Biol.
  • S-EIA Stick Enzyme Immunoassay
  • Solid-phase Immunobead Assay The solid phase consisted of a paddle made of a bamboo stick coated with organic base solvent correction fluid supplied by Pentel of America, Ltd., Torrance CA 905034. Immunobead consisted of blue colored latex, 0.314 ⁇ in diameter, supplied by Seradyn, Inc., Particle Technology Division Ind., IN.
  • liquid-paper-coated sticks were examined as-is or were coated with a 1% wt/wt solution of Human Serum Albumin (HSA), then examined as non-Me- OH-fixed or MeOH-fixed with the MAb-CTX-colored late.
  • HSA Human Serum Albumin
  • MAb-CTX was coated onto liquid-paper sticks and then examined with MAb-CTX-color latex.
  • the immunobead was mixed thoroughly before use.
  • Solid-phase Immunoassay An inch-deep incision is made into the filet portion of the fish near the head region, as shown below.
  • the coated paddle end is inserted into the incision and pushed up and down to touch the fish tissue.
  • the paddle is removed, air-dried, and fixed quickly (1-3 seconds) with absolute methanol. After air-drying, the coated methanol-fixed end of the paddle is immersed into 0.5 ml of the immunobead color suspension. After 2, 5, and 10 minutes, the stick is examined and washed in saline. Any fish giving the stick a distinct coloration after 5 minutes is considered positive. If negative, immersion is continued up to 10 minutes.
  • Table 1 shows the species, source and the test values given by the S-EIA and SPIA procedures for fish implicated in ciguatera poisoning. All samples were obtained from the Department of Health, State of Hawaii , except for two specimens (Antigua and California) . The S-EIA values were all in the rejection category and in complete agreement with the SPIA results . The majority of the toxic fishes were from the Big Island of Hawaii and associated with the Caranx sp.
  • Table 3 summarizes the comparison of S-EIA and SPIA of the two studies, totalling 436 fishes .
  • Table 4 shows the preliminary data obtained by a voluntary sportsfisherman assessing the solid- phase immunoassay presented earlier.
  • Mr. Dale Takata of the Honolulu City Water and Waste management is summarized.
  • Mr. Takata volunteered to use the new SPIA test.
  • 37 negative and borderline fishes were eaten, and 18 were rejected as being positive.
  • Consumption of two fishes (papio and kole) from the positive group caused ciguatera poisoning in two individuals who were warned of the fish's toxicity based on the SPIA test.
  • the SPIA method described should be applicable to other antigen-antibody systems, especially when the antigen is an epitope or hapten of low molecular weight.

Abstract

Cette invention concerne un dosage immunologique très simplifié s'effectuant en phase solide, réalisé avec des billes colorées en latex, qui sert à détecter la ciguatoxine et des polyéthers apparentés. Ce procédé a été comparé avec un dosage immunologique réalisé antérieurement, effectué à l'aide d'un bâtonnet d'enzyme. L'analyse par le chi carré de deux expériences distinctes portant sur 153 et 283 poissons d'espèces différentes ont donné des valeurs X2 de p < 0,001 et p < 0,005 respectivement. La concordance entre les procédés portant sur 26 poissons concernés par l'empoisonnement au ciguatera était de 100 %. Une évaluation préliminaire dans ce domaine a donné des résultats encourageants. Le procédé est simple et il peut s'appliquer à ce domaine d'utilisation. En outre, ce procédé pourrait s'appliquer à d'autres détections d'anticorps et d'antigènes, plus particulièrement celles concernant les déterminants à faible dalton.
PCT/US1991/002703 1990-04-20 1991-04-19 Analyse immunologique simplifiee en phase solide effectuee a l'aide de billes colorees pour detecter la ciguatoxine ou des polyethers apparentes WO1991016631A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BR919106366A BR9106366A (pt) 1990-04-20 1991-04-19 Imunoensaio por imunoconta em fase solida simplificado para deteccao de ciguatoxima e polieteres correlatos
JP91508651A JPH05507554A (ja) 1990-04-20 1991-04-19 シグアトキシンおよび関連ポリエーテル類検出用簡易化固相イムノビードアッセイ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51131790A 1990-04-20 1990-04-20
US511,317 1990-04-20

Publications (1)

Publication Number Publication Date
WO1991016631A1 true WO1991016631A1 (fr) 1991-10-31

Family

ID=24034379

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/002703 WO1991016631A1 (fr) 1990-04-20 1991-04-19 Analyse immunologique simplifiee en phase solide effectuee a l'aide de billes colorees pour detecter la ciguatoxine ou des polyethers apparentes

Country Status (6)

Country Link
EP (1) EP0528895A4 (fr)
JP (1) JPH05507554A (fr)
AU (1) AU647125B2 (fr)
BR (1) BR9106366A (fr)
CA (1) CA2080925A1 (fr)
WO (1) WO1991016631A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0638170A1 (fr) * 1992-05-01 1995-02-15 Hawaii Chemtect Incorporated Extraction rapide de la ciguatoxine de tissus contamines

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101963614A (zh) * 2010-09-03 2011-02-02 青岛科技大学 毛细管电泳电化学酶联免疫分析检测雪卡毒素的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703017A (en) * 1984-02-14 1987-10-27 Becton Dickinson And Company Solid phase assay with visual readout
US4816392A (en) * 1984-10-02 1989-03-28 Research Corporation Of The University Of Hawaii Rapid stick test for detection of ciguatoxin and other polyether toxins from tissues

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703017A (en) * 1984-02-14 1987-10-27 Becton Dickinson And Company Solid phase assay with visual readout
US4703017C1 (en) * 1984-02-14 2001-12-04 Becton Dickinson Co Solid phase assay with visual readout
US4816392A (en) * 1984-10-02 1989-03-28 Research Corporation Of The University Of Hawaii Rapid stick test for detection of ciguatoxin and other polyether toxins from tissues

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
S. NATORI et al., "Mycotoxins and Phycotoxins '88", published 1989 by Elsevier Science Publishers (The Netherlands), see pages 303-310, especially see "Summary", on page 303 and "Discussion" on pages 307-308. *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0638170A1 (fr) * 1992-05-01 1995-02-15 Hawaii Chemtect Incorporated Extraction rapide de la ciguatoxine de tissus contamines
EP0638170A4 (fr) * 1992-05-01 1996-07-17 Hawaii Chemtect Inc Extraction rapide de la ciguatoxine de tissus contamines.
EP0933636A1 (fr) * 1992-05-01 1999-08-04 Hawaii Chemtect Incorporated Extraction rapide de la ciguatoxine de tissus contaminés

Also Published As

Publication number Publication date
CA2080925A1 (fr) 1991-10-21
AU647125B2 (en) 1994-03-17
BR9106366A (pt) 1993-04-27
AU7789191A (en) 1991-11-11
JPH05507554A (ja) 1993-10-28
EP0528895A4 (en) 1993-03-10
EP0528895A1 (fr) 1993-03-03

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