WO1991015750A1 - Dispositif microfabrique pour le triage de cellules biologiques - Google Patents

Dispositif microfabrique pour le triage de cellules biologiques Download PDF

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Publication number
WO1991015750A1
WO1991015750A1 PCT/GB1991/000542 GB9100542W WO9115750A1 WO 1991015750 A1 WO1991015750 A1 WO 1991015750A1 GB 9100542 W GB9100542 W GB 9100542W WO 9115750 A1 WO9115750 A1 WO 9115750A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
microfabricated
cell sorting
biological cell
microfabricated device
Prior art date
Application number
PCT/GB1991/000542
Other languages
English (en)
Inventor
Paul Henry Kaye
Mark Christopher Tracey
Original Assignee
Carri-Med Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carri-Med Limited filed Critical Carri-Med Limited
Publication of WO1991015750A1 publication Critical patent/WO1991015750A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Definitions

  • This invention relates to the sorting of biological cells into spatially separate sub groups.
  • the criteria for sorting may be applied to measurements obtained by any technique or techniques suitable for microfabricated or electronic implementation on a substrate.
  • Microfabrication and microelectronic techniques offer a number of actual and potential measurement techniques that may be applied to characterize biological cells.
  • PCT/GB91/00289 and morphological measurements.
  • morphological measurements When suitably implemented such techniques can be applied on a cell by cell basis.
  • microelectromechani ⁇ al structures and their formation by selective CVD techniques, are disclosed in: "Selective Chemical Vapor Deposition of Tungsten for Mi ⁇ roelectromechani ⁇ al Structures" by N. C. MACDONALD et al. , Sensors and Actuators, 20 (1989) 123-133.
  • the so-called “microtweezers” are activated by control voltages applied to electrodes.
  • such a machine is implemented by utilising a microfabricated moveable structure, preferably microfabricated beams, to direct cells between distinct spatial locations.
  • FIG. 1 is a topologi ⁇ al plan view of a substrate on which a cell sorter is formed.
  • the cells to be sorted whose diameters may be only a few micrometres, are fed sequentially from an entry port 1, via a guiding structure 2, past a generalised sensing device or zone 3.
  • the deflectable beams 4, 5, 6, are set into appropriate states to direct the cell into a particular destination, for example, a hole 7, 8, 9 or 10 etched through the substrate.
  • the destination is structure 9.
  • the beams, along with associated bifurcations can be extended to a tree structure of ' n' levels thereby yielding 2 possible sorting sub ranges.
  • the absolute lengths of the channels are as short as possible while maintaining compatibility with interconnecting structures.
  • the shafts 1, 3, 7-10 may be fabricated by selective etching as disclosed in the copending International Patent Application referred to above; the etched substrate is closed by a glass/silica cover, thereby forming the channels as tunnels.
  • Typical dimensions of the entry port 1 are 500 ⁇ m or less square; of the sensing zone about 100 ⁇ m square; of the channels about 5 to 10 ⁇ m in width and depth; of the holes (shafts) 7 - 10 about 500 ⁇ m or less square; and of the beams, which are typically of constant rectangular section, about 150 ⁇ m long by 3 ⁇ m square.
  • the overall dimensions of the device could be l ⁇ m square.
  • the beams are activated by selective control voltages applied as signals for control circuitry (not shown) operated, for example, under the control of a microprocessor or other computer.
  • the computer is programmed to control the beams' deflection in response to the desired cell-sorting procedural steps.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Fluid Mechanics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Appareil de triage de cellules en sous-groupes séparés dans l'espace, comprenant une structure mobile microfabriquée (4, 5, 6) servant à diriger les cellules entre des emplacements spatiaux distincts.
PCT/GB1991/000542 1990-04-09 1991-04-08 Dispositif microfabrique pour le triage de cellules biologiques WO1991015750A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909008044A GB9008044D0 (en) 1990-04-09 1990-04-09 Microfabricated device for biological cell sorting
GB9008044.1 1990-04-09

Publications (1)

Publication Number Publication Date
WO1991015750A1 true WO1991015750A1 (fr) 1991-10-17

Family

ID=10674166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/000542 WO1991015750A1 (fr) 1990-04-09 1991-04-08 Dispositif microfabrique pour le triage de cellules biologiques

Country Status (2)

Country Link
GB (1) GB9008044D0 (fr)
WO (1) WO1991015750A1 (fr)

Cited By (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022054A1 (fr) * 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Analyse fondee sur un resserrement du systeme d'ecoulment
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
EP0616218A1 (fr) * 1993-03-16 1994-09-21 Hitachi, Ltd. Dispositif micro-réacteur et système d'analyse de très petits échantillons utilisant ce dispositif
US5427946A (en) * 1992-05-01 1995-06-27 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
DE19520298A1 (de) * 1995-06-02 1996-12-05 Bayer Ag Sortiervorrichtung für biologische Zellen oder Viren
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5747349A (en) * 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
WO1998045683A1 (fr) * 1997-04-08 1998-10-15 Smithkline Beecham Plc Dispositif servant a isoler de petites perles polymeres depuis une suspension de ces perles
US5906732A (en) * 1995-11-17 1999-05-25 Yazaki Corporation Apparatus for separating minute substances in liquid
US5950842A (en) * 1995-02-23 1999-09-14 Natec, Reich, Summer Gmbh & Co. Kg Line connector with 90 degree rotation mechanism
WO1999061888A2 (fr) * 1998-05-22 1999-12-02 California Institute Of Technology Trieur de cellules microfabrique
US6056860A (en) * 1996-09-18 2000-05-02 Aclara Biosciences, Inc. Surface modified electrophoretic chambers
US6193647B1 (en) 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
WO2001019516A1 (fr) * 1999-09-13 2001-03-22 Hoffmann La Roche Systeme de manipulation de suspensions
WO2001049412A1 (fr) * 1999-12-30 2001-07-12 Acreo Ab Procede et dispositif de regulation de debit
WO2002001189A1 (fr) * 2000-06-26 2002-01-03 Gnothis Holding S.A. Procede de selection de particules
US6432630B1 (en) 1996-09-04 2002-08-13 Scandinanian Micro Biodevices A/S Micro-flow system for particle separation and analysis
WO2003025563A1 (fr) * 2001-09-16 2003-03-27 Chemometec A/S Procede et systeme de detection et eventuellement d'isolement de particules peu abondantes
US6540895B1 (en) * 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
WO2003060486A1 (fr) * 2002-01-10 2003-07-24 Board Of Regents, The University Of Texas System Systeme de tri de flux et procedes associes
WO2003078065A1 (fr) * 2002-03-14 2003-09-25 Micronics, Inc. Dispositif microfluidique a reseau de canaux
EP1499453A1 (fr) * 2002-04-17 2005-01-26 Cytonome, Inc. Procede et appareil de tri de particules
EP1567845A2 (fr) * 2002-07-08 2005-08-31 John S. Foster Procede et appareil destines a trier des cellules biologiques au moyen d'un dispositif a systeme mecanique microelectrique
US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO2007084392A2 (fr) * 2006-01-13 2007-07-26 Micronics, Inc. Soupapes a actionnement electromagnetique a utiliser dans des structures microfluidiques
EP1998884A2 (fr) * 2006-03-16 2008-12-10 Visiongate, Inc. Appareil à injecteur de flux coaxial en porte-à-faux et procédé de tri de particules
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7749737B2 (en) 2003-04-03 2010-07-06 Fluidigm Corporation Thermal reaction device and method for using the same
US7820427B2 (en) 2001-11-30 2010-10-26 Fluidigm Corporation Microfluidic device and methods of using same
US7833708B2 (en) 2001-04-06 2010-11-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US7867454B2 (en) 2003-04-03 2011-01-11 Fluidigm Corporation Thermal reaction device and method for using the same
US7963399B2 (en) 2002-04-17 2011-06-21 Cytonome/St, Llc Method and apparatus for sorting particles
US8007746B2 (en) 2003-04-03 2011-08-30 Fluidigm Corporation Microfluidic devices and methods of using same
US8129176B2 (en) 2000-06-05 2012-03-06 California Institute Of Technology Integrated active flux microfluidic devices and methods
US8623295B2 (en) 2002-04-17 2014-01-07 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US8658418B2 (en) 2002-04-01 2014-02-25 Fluidigm Corporation Microfluidic particle-analysis systems
WO2014094791A1 (fr) * 2012-12-20 2014-06-26 Danmarks Tekniske Universitet Dispositif et méthode d'analyse de composition de fluide
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
US9339850B2 (en) 2002-04-17 2016-05-17 Cytonome/St, Llc Method and apparatus for sorting particles
US9714443B2 (en) 2002-09-25 2017-07-25 California Institute Of Technology Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US10994273B2 (en) 2004-12-03 2021-05-04 Cytonome/St, Llc Actuation of parallel microfluidic arrays

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EP0177718A2 (fr) * 1984-09-11 1986-04-16 Partec AG Procédé et dispositif pour le triage de particules microscopiques
US4676274A (en) * 1985-02-28 1987-06-30 Brown James F Capillary flow control

Patent Citations (2)

* Cited by examiner, † Cited by third party
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EP0177718A2 (fr) * 1984-09-11 1986-04-16 Partec AG Procédé et dispositif pour le triage de particules microscopiques
US4676274A (en) * 1985-02-28 1987-06-30 Brown James F Capillary flow control

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Title
PROCEEDINGS OF THE IEEE. vol. 70, no. 5, May 1982, NEW YORK US pages 420 - 457; K E PETERSEN: 'Silicon as a mechanical material ' see page 434 - page 435 see page 442 - page 443 see page 446 - page 449 *
REVIEW OF SCIENTIFIC INSTRUMENTS. vol. 51, no. 1, January 1980, NEW YORK US pages 111 - 115; P LENZ: 'Mechanical cell separator ' see page 111 - page 113 *

Cited By (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955029A (en) * 1992-05-01 1999-09-21 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5427946A (en) * 1992-05-01 1995-06-27 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US6184029B1 (en) 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US7494770B2 (en) 1992-05-01 2009-02-24 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification analysis
US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5635358A (en) * 1992-05-01 1997-06-03 Trustees Of The University Of Pennsylvania Fluid handling methods for use in mesoscale analytical devices
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US7892819B2 (en) 1992-05-01 2011-02-22 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO1993022054A1 (fr) * 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Analyse fondee sur un resserrement du systeme d'ecoulment
US6660517B1 (en) 1992-05-01 2003-12-09 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5928880A (en) * 1992-05-01 1999-07-27 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5480614A (en) * 1993-03-16 1996-01-02 Hitachi, Ltd. Micro-reactor device for minute sample analysis
EP0616218A1 (fr) * 1993-03-16 1994-09-21 Hitachi, Ltd. Dispositif micro-réacteur et système d'analyse de très petits échantillons utilisant ce dispositif
US5950842A (en) * 1995-02-23 1999-09-14 Natec, Reich, Summer Gmbh & Co. Kg Line connector with 90 degree rotation mechanism
US5837200A (en) * 1995-06-02 1998-11-17 Bayer Aktiengesellschaft Sorting device for biological cells or viruses
DE19520298A1 (de) * 1995-06-02 1996-12-05 Bayer Ag Sortiervorrichtung für biologische Zellen oder Viren
US5906732A (en) * 1995-11-17 1999-05-25 Yazaki Corporation Apparatus for separating minute substances in liquid
US5747349A (en) * 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
US6432630B1 (en) 1996-09-04 2002-08-13 Scandinanian Micro Biodevices A/S Micro-flow system for particle separation and analysis
US7138269B2 (en) 1996-09-04 2006-11-21 Inverness Medical Switzerland Gmbh Microflow system for particle separation and analysis
US6056860A (en) * 1996-09-18 2000-05-02 Aclara Biosciences, Inc. Surface modified electrophoretic chambers
US6332540B1 (en) 1997-04-08 2001-12-25 Smithkline Beecham P.L.C. Device for isolating small polymeric beads from a suspension of such beads
WO1998045683A1 (fr) * 1997-04-08 1998-10-15 Smithkline Beecham Plc Dispositif servant a isoler de petites perles polymeres depuis une suspension de ces perles
US6540895B1 (en) * 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
WO1999061888A3 (fr) * 1998-05-22 2001-12-13 California Inst Of Techn Trieur de cellules microfabrique
AU763433B2 (en) * 1998-05-22 2003-07-24 California Institute Of Technology Microfabricated cell sorter
WO1999061888A2 (fr) * 1998-05-22 1999-12-02 California Institute Of Technology Trieur de cellules microfabrique
US6695765B1 (en) 1999-04-08 2004-02-24 The Board Of Trustees Of The University Of Illinois Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
US6193647B1 (en) 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
WO2001019516A1 (fr) * 1999-09-13 2001-03-22 Hoffmann La Roche Systeme de manipulation de suspensions
WO2001049412A1 (fr) * 1999-12-30 2001-07-12 Acreo Ab Procede et dispositif de regulation de debit
US8129176B2 (en) 2000-06-05 2012-03-06 California Institute Of Technology Integrated active flux microfluidic devices and methods
US8257666B2 (en) 2000-06-05 2012-09-04 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002001189A1 (fr) * 2000-06-26 2002-01-03 Gnothis Holding S.A. Procede de selection de particules
DE10031028B4 (de) * 2000-06-26 2008-09-04 Gnothis Holding Sa Verfahren zur Selektion von Partikeln
US9926521B2 (en) 2000-06-27 2018-03-27 Fluidigm Corporation Microfluidic particle-analysis systems
US8936764B2 (en) 2001-04-06 2015-01-20 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US7833708B2 (en) 2001-04-06 2010-11-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US8486636B2 (en) 2001-04-06 2013-07-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
WO2003025563A1 (fr) * 2001-09-16 2003-03-27 Chemometec A/S Procede et systeme de detection et eventuellement d'isolement de particules peu abondantes
US8163492B2 (en) 2001-11-30 2012-04-24 Fluidign Corporation Microfluidic device and methods of using same
US8343442B2 (en) 2001-11-30 2013-01-01 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US9643178B2 (en) 2001-11-30 2017-05-09 Fluidigm Corporation Microfluidic device with reaction sites configured for blind filling
US7820427B2 (en) 2001-11-30 2010-10-26 Fluidigm Corporation Microfluidic device and methods of using same
US7837946B2 (en) 2001-11-30 2010-11-23 Fluidigm Corporation Microfluidic device and methods of using same
US7452725B2 (en) 2002-01-10 2008-11-18 Board Of Regents, The University Of Texas System Flow sorting system and methods regarding same
WO2003060486A1 (fr) * 2002-01-10 2003-07-24 Board Of Regents, The University Of Texas System Systeme de tri de flux et procedes associes
WO2003078065A1 (fr) * 2002-03-14 2003-09-25 Micronics, Inc. Dispositif microfluidique a reseau de canaux
WO2003078972A1 (fr) * 2002-03-14 2003-09-25 Micronics, Inc. Cytometrie en flux sur ruban et triage de cellules
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
US8658418B2 (en) 2002-04-01 2014-02-25 Fluidigm Corporation Microfluidic particle-analysis systems
US9550215B2 (en) 2002-04-17 2017-01-24 Cytonome/St, Llc Method and apparatus for sorting particles
US10029283B2 (en) 2002-04-17 2018-07-24 Cytonome/St, Llc Method and apparatus for sorting particles
US10029263B2 (en) 2002-04-17 2018-07-24 Cytonome/St, Llc Method and apparatus for sorting particles
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
EP1499453A1 (fr) * 2002-04-17 2005-01-26 Cytonome, Inc. Procede et appareil de tri de particules
US9339850B2 (en) 2002-04-17 2016-05-17 Cytonome/St, Llc Method and apparatus for sorting particles
EP1499453A4 (fr) * 2002-04-17 2009-11-04 Cytonome Inc Procede et appareil de tri de particules
US11027278B2 (en) 2002-04-17 2021-06-08 Cytonome/St, Llc Methods for controlling fluid flow in a microfluidic system
US10427159B2 (en) 2002-04-17 2019-10-01 Cytonome/St, Llc Microfluidic device
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