WO1990011523A3 - Depôt de rapport par catalysation - Google Patents

Depôt de rapport par catalysation Download PDF

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Publication number
WO1990011523A3
WO1990011523A3 PCT/US1990/001569 US9001569W WO9011523A3 WO 1990011523 A3 WO1990011523 A3 WO 1990011523A3 US 9001569 W US9001569 W US 9001569W WO 9011523 A3 WO9011523 A3 WO 9011523A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
reporter
conjugate
deposition
activation system
Prior art date
Application number
PCT/US1990/001569
Other languages
English (en)
Other versions
WO1990011523A2 (fr
Inventor
Mark Norman Bobrow
Richard Calvin Ebersole
Gerald Joseph Litt
John Richard Moran
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Priority to DE69007473T priority Critical patent/DE69007473T2/de
Priority to SU5001818A priority patent/RU2102759C1/ru
Priority to AT90905997T priority patent/ATE103071T1/de
Publication of WO1990011523A2 publication Critical patent/WO1990011523A2/fr
Publication of WO1990011523A3 publication Critical patent/WO1990011523A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Abstract

La présente invention concerne un procédé permettant de catalyser un dépôt de rapport pour mieux détecter ou quantifier un élément d'analyse se trouvant dans un échantillon, par l'amplification du signal de détection. Le procédé consiste à immobiliser un système d'activation par enzyme dépendant de l'élément d'analyse qui catalyse le dépôt de rapport en activant un conjugué consistant en un substrat étiqueté de manière décelable et spécifique pour ledit système d'enzyme. Ledit conjugué réagit avec le système d'activation par enzyme dépendant de l'élément d'analyse pour former un conjugué activé qui se dépose sensiblement partout là où le récepteur pour le conjugué activé est immobilisé, ledit récepteur ne réagissant pas avec le système d'activation par enzyme. Dans un autre mode de réalisation, l'invention décrit un procédé d'analyse permettant de détecter ou de quantifier la présence ou l'absence d'un élément d'analyse dans un échantillon utilisant le dépôt de rapport par catalysation pour amplifier le signal de rapport. On décrit également des composés nouveaux que l'on peut utiliser comme agents de réaction pour préparer des conjugués de type HABA.
PCT/US1990/001569 1989-03-29 1990-03-28 Depôt de rapport par catalysation WO1990011523A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE69007473T DE69007473T2 (de) 1989-03-29 1990-03-28 Katalysierte ablagerung von markierungsmittel.
SU5001818A RU2102759C1 (ru) 1989-03-29 1990-03-28 Способ обнаружения или количественного определения аналита в образце
AT90905997T ATE103071T1 (de) 1989-03-29 1990-03-28 Katalysierte ablagerung von markierungsmittel.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33035789A 1989-03-29 1989-03-29
US330,357 1989-03-29
US49422690A 1990-03-20 1990-03-20
US494,226 1990-03-20

Publications (2)

Publication Number Publication Date
WO1990011523A2 WO1990011523A2 (fr) 1990-10-04
WO1990011523A3 true WO1990011523A3 (fr) 1991-02-21

Family

ID=26987247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/001569 WO1990011523A2 (fr) 1989-03-29 1990-03-28 Depôt de rapport par catalysation

Country Status (7)

Country Link
EP (1) EP0465577B1 (fr)
JP (1) JP2948904B2 (fr)
AU (1) AU645491B2 (fr)
DE (1) DE69007473T2 (fr)
ES (1) ES2063347T3 (fr)
RU (1) RU2102759C1 (fr)
WO (1) WO1990011523A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0795610A1 (fr) * 1996-03-13 1997-09-17 Becton, Dickinson and Company Amplification de signal d'hybrisation "in situ" par dépÔt de tyramine biotinilée
GB9712818D0 (en) * 1996-07-08 1997-08-20 Cambridge Antibody Tech Labelling and selection of specific binding molecules
US6537441B1 (en) 1997-06-04 2003-03-25 Australian Membrane And Biotechnology Research Biosensor involving the use of optically sensitive moieties
DE10015418C2 (de) * 2000-03-28 2001-04-19 Eisenblaetter Gerd Gmbh Schleifwerkzeug und Verfahren zu dessen Herstellung
US8758989B2 (en) * 2006-04-06 2014-06-24 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US9469868B2 (en) 2008-11-17 2016-10-18 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using covalent bond forming reactive pairs
WO2010056577A1 (fr) 2008-11-17 2010-05-20 Magic Technologies, Inc. Procédés et compositions de détection à base de particules de molécules cibles utilisant des molécules de liaison
EP2675917B1 (fr) 2011-02-16 2017-09-13 Headway Technologies, Inc. Procédés et compositions pour l'ancrage localisé sur un marqueur détectable
US10041950B2 (en) 2012-03-27 2018-08-07 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
TWI653449B (zh) 2018-01-31 2019-03-11 國立交通大學 光子晶體與磁珠結合的螢光免疫檢測裝置和方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984002193A1 (fr) * 1982-12-03 1984-06-07 Du Pont Analyse immunologique a support chromogene
EP0144744A2 (fr) * 1983-11-30 1985-06-19 Miles Laboratories, Inc. Essai de liaison spécifique basé sur l'amplification par cascade enzymatique

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984002193A1 (fr) * 1982-12-03 1984-06-07 Du Pont Analyse immunologique a support chromogene
EP0144744A2 (fr) * 1983-11-30 1985-06-19 Miles Laboratories, Inc. Essai de liaison spécifique basé sur l'amplification par cascade enzymatique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Journal of Immunological Methods, Volume 125, 1989, Elsevier Science Publishers B.V. (Biomedical Division) (Amsterdam, NL), M.N. BOBROW et al.: "Catalyzed Reporter Deposition, a Novel Method of Signal Amplification. Application to Immunoassays", pages 279-285 see the whole article *

Also Published As

Publication number Publication date
EP0465577B1 (fr) 1994-03-16
JP2948904B2 (ja) 1999-09-13
DE69007473D1 (de) 1994-04-21
AU645491B2 (en) 1994-01-20
WO1990011523A2 (fr) 1990-10-04
RU2102759C1 (ru) 1998-01-20
ES2063347T3 (es) 1995-01-01
JPH04504206A (ja) 1992-07-30
AU5410190A (en) 1990-10-22
DE69007473T2 (de) 1994-07-21
EP0465577A1 (fr) 1992-01-15

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