WO1990006045A3 - Sondes d'acide nucleique - Google Patents

Sondes d'acide nucleique Download PDF

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Publication number
WO1990006045A3
WO1990006045A3 PCT/EP1989/001419 EP8901419W WO9006045A3 WO 1990006045 A3 WO1990006045 A3 WO 1990006045A3 EP 8901419 W EP8901419 W EP 8901419W WO 9006045 A3 WO9006045 A3 WO 9006045A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
particles
acid probes
disclosed
monodisperse
Prior art date
Application number
PCT/EP1989/001419
Other languages
English (en)
Other versions
WO1990006045A2 (fr
Inventor
Erik Hornes
Lars Korsnes
Original Assignee
Holmes Michael John
Dynal As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888827157A external-priority patent/GB8827157D0/en
Priority claimed from GB888827160A external-priority patent/GB8827160D0/en
Priority claimed from GB888827159A external-priority patent/GB8827159D0/en
Priority claimed from GB888827166A external-priority patent/GB8827166D0/en
Priority claimed from GB888827167A external-priority patent/GB8827167D0/en
Priority claimed from GB888827158A external-priority patent/GB8827158D0/en
Priority claimed from GB898906643A external-priority patent/GB8906643D0/en
Application filed by Holmes Michael John, Dynal As filed Critical Holmes Michael John
Priority to DE68913555T priority Critical patent/DE68913555T2/de
Priority to AT90900184T priority patent/ATE102256T1/de
Publication of WO1990006045A2 publication Critical patent/WO1990006045A2/fr
Publication of WO1990006045A3 publication Critical patent/WO1990006045A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne des particules monodispersées supraparamagnétiques portant une pluralité de molécules d'un oligonucléotide, que l'on peut utiliser inter alia pour le séquençage d'acides nucléiques monocaténaires. On peut fixer les oligonucléotides par covalence ou les lier par affinité aux particules soit par leur terminaison 3' soit par leur terminaison 5'. Lesdites particules ont une masse volumique comprise dans une plage allant de 1,1 à 1,8 et ont un diamètre compris entre 1 to 10 microns. L'invention concerne également un kit d'isolation et/ou de traitement d'acide nucléique cible.
PCT/EP1989/001419 1988-11-21 1989-11-21 Sondes d'acide nucleique WO1990006045A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE68913555T DE68913555T2 (de) 1988-11-21 1989-11-21 Sonden aus nukleinsäuren.
AT90900184T ATE102256T1 (de) 1988-11-21 1989-11-21 Sonden aus nukleinsaeuren.

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
GB8827167.1 1988-11-21
GB8827160.6 1988-11-21
GB888827157A GB8827157D0 (en) 1988-11-21 1988-11-21 Sequencing nucleic acids
GB888827160A GB8827160D0 (en) 1988-11-21 1988-11-21 Detection & quantitative determination of rna & dna
GB8827158.0 1988-11-21
GB888827159A GB8827159D0 (en) 1988-11-21 1988-11-21 Subtraction cdna library construction
GB8827159.8 1988-11-21
GB888827166A GB8827166D0 (en) 1988-11-21 1988-11-21 Obtention & purification of rna
GB888827167A GB8827167D0 (en) 1988-11-21 1988-11-21 In vitro mutagenesis
GB888827158A GB8827158D0 (en) 1988-11-21 1988-11-21 Process for producing cdna
GB898906643A GB8906643D0 (en) 1989-03-22 1989-03-22 Sequencing nucleic acids
GB8906643.5 1989-03-22
GB8827157.2 1989-03-22
GB8827166.3 1989-03-22

Publications (2)

Publication Number Publication Date
WO1990006045A2 WO1990006045A2 (fr) 1990-06-14
WO1990006045A3 true WO1990006045A3 (fr) 1991-03-21

Family

ID=27562808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1989/001419 WO1990006045A2 (fr) 1988-11-21 1989-11-21 Sondes d'acide nucleique

Country Status (7)

Country Link
EP (1) EP0446260B1 (fr)
JP (1) JP3020271B2 (fr)
AT (1) ATE102256T1 (fr)
AU (1) AU640626B2 (fr)
CA (1) CA2003508C (fr)
DE (1) DE68913555T2 (fr)
WO (1) WO1990006045A2 (fr)

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EP0446260A1 (fr) 1991-09-18
CA2003508C (fr) 2000-01-25
JPH04501959A (ja) 1992-04-09
AU4758690A (en) 1990-06-26
CA2003508A1 (fr) 1990-05-21
ATE102256T1 (de) 1994-03-15
WO1990006045A2 (fr) 1990-06-14
DE68913555T2 (de) 1994-07-07
JP3020271B2 (ja) 2000-03-15
AU640626B2 (en) 1993-09-02
DE68913555D1 (de) 1994-04-07
EP0446260B1 (fr) 1994-03-02

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