WO1990000601A3 - Novel glucose isomerase enzymes and their use - Google Patents

Novel glucose isomerase enzymes and their use Download PDF

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Publication number
WO1990000601A3
WO1990000601A3 PCT/EP1989/000839 EP8900839W WO9000601A3 WO 1990000601 A3 WO1990000601 A3 WO 1990000601A3 EP 8900839 W EP8900839 W EP 8900839W WO 9000601 A3 WO9000601 A3 WO 9000601A3
Authority
WO
WIPO (PCT)
Prior art keywords
glucose isomerase
glucose
amino acid
novel glucose
isomerase enzymes
Prior art date
Application number
PCT/EP1989/000839
Other languages
French (fr)
Other versions
WO1990000601A2 (en
Inventor
Rudolf Gijsbertus Marie Luiten
Wilhelmus Johannes Quax
Paul William Schuurhuizen
Nadir Mrabet
Original Assignee
Gist Brocades Nv
Plant Genetic Systems Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv, Plant Genetic Systems Nv filed Critical Gist Brocades Nv
Priority to US07/398,706 priority Critical patent/US5290690A/en
Publication of WO1990000601A2 publication Critical patent/WO1990000601A2/en
Publication of WO1990000601A3 publication Critical patent/WO1990000601A3/en
Priority to FI901279A priority patent/FI102541B1/en
Priority to DK199000662A priority patent/DK175633B1/en
Priority to KR90700559A priority patent/KR0140866B1/en
Priority to BG91484A priority patent/BG60322B2/en
Priority to US07/640,476 priority patent/US5376536A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.
PCT/EP1989/000839 1988-07-15 1989-07-17 Novel glucose isomerase enzymes and their use WO1990000601A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US07/398,706 US5290690A (en) 1988-07-15 1989-08-25 Methods and means for controlling the stability of proteins
FI901279A FI102541B1 (en) 1988-07-15 1990-03-14 New glucose isomerase enzymes and their use
DK199000662A DK175633B1 (en) 1988-07-15 1990-03-14 Modified glucose isomerase, immobilized modified glucose isomerase and use of these enzymes in the production of fructose syrups
KR90700559A KR0140866B1 (en) 1988-07-15 1990-03-15 Novel glucose isomerase enzymes and their use
BG91484A BG60322B2 (en) 1988-07-15 1990-03-15 New glucosaisomerase enzymes and their use
US07/640,476 US5376536A (en) 1988-07-15 1991-01-10 Glucose isomerase enzymes and their use

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP88201539 1988-07-15
EP88201539.9 1988-07-15
EP88402789.7 1988-11-04

Publications (2)

Publication Number Publication Date
WO1990000601A2 WO1990000601A2 (en) 1990-01-25
WO1990000601A3 true WO1990000601A3 (en) 1990-02-08

Family

ID=8199823

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1989/000839 WO1990000601A2 (en) 1988-07-15 1989-07-17 Novel glucose isomerase enzymes and their use

Country Status (1)

Country Link
WO (1) WO1990000601A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0028942A2 (en) * 1979-11-13 1981-05-20 Uop Inc. Process for the production of an immobilized enzyme system and the purification of glucose isomerase
US4410627A (en) * 1982-06-30 1983-10-18 Nabisco Brands, Inc. Glucose isomerase process
WO1989001520A1 (en) * 1987-08-11 1989-02-23 Cetus Corporation Procaryotic xylose isomerase muteins and method to increase protein stability

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0028942A2 (en) * 1979-11-13 1981-05-20 Uop Inc. Process for the production of an immobilized enzyme system and the purification of glucose isomerase
US4410627A (en) * 1982-06-30 1983-10-18 Nabisco Brands, Inc. Glucose isomerase process
WO1989001520A1 (en) * 1987-08-11 1989-02-23 Cetus Corporation Procaryotic xylose isomerase muteins and method to increase protein stability

Also Published As

Publication number Publication date
WO1990000601A2 (en) 1990-01-25

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