WO1984003698A1 - Compound - Google Patents

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Publication number
WO1984003698A1
WO1984003698A1 PCT/SE1984/000089 SE8400089W WO8403698A1 WO 1984003698 A1 WO1984003698 A1 WO 1984003698A1 SE 8400089 W SE8400089 W SE 8400089W WO 8403698 A1 WO8403698 A1 WO 8403698A1
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WO
WIPO (PCT)
Prior art keywords
compound
group
fluorescence
lanthanide
bio
Prior art date
Application number
PCT/SE1984/000089
Other languages
French (fr)
Inventor
Heikki Mikola
Veli-Matti Mukkala
Ilkka Hemmilae
Original Assignee
Lkb Produkter Ab
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Lkb Produkter Ab, Wallac Oy filed Critical Lkb Produkter Ab
Priority to DE8484901128T priority Critical patent/DE3462252D1/en
Publication of WO1984003698A1 publication Critical patent/WO1984003698A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C229/06—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton
    • C07C229/10—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings
    • C07C229/16—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings to carbon atoms of hydrocarbon radicals substituted by amino or carboxyl groups, e.g. ethylenediamine-tetra-acetic acid, iminodiacetic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
    • C07F9/3808—Acyclic saturated acids which can have further substituents on alkyl
    • C07F9/3817—Acids containing the structure (RX)2P(=X)-alk-N...P (X = O, S, Se)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/40—Rare earth chelates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Definitions

  • the present invention refers to a compound.
  • Immunoassay is a field in which the sensitivity of the analysis method often is of decisive importance as the amount of analyte in different biological liquids usually is very low.
  • radioisotopes have been widely used as labels in immunoassays despite the dis- advantages caused by their use.
  • a very intense research has been carried out with the aim of replacing the radioisotopes with labels giving at least the same or a higher sensitivity than the isotopes.
  • Fluorescent molecules have in these connections been presented as one of the most potential alternatives to radioisotopes.
  • Comprehensive surveys have recently been published, which give a good general view of fluoroimmunoanalytical determinations known at present (see Smith et al. (1981) Ann. Clin. Biochem. 18, 253-274, Ullman (1981) "Recent Advances in Fluorescence Immunoassay techniques").
  • time-resolved fluorescence makes it, however, possible to separate the specific fluorescence of the label from the disturbing, unspecific background fluorescence.
  • time-resolved fluorescence when following biospecific affinity reactions is described in the U.S. Patent No. 4,374,120 and the European patent application No. 82850077.7.
  • time-resolved fluorescence the fluorescent label is excited by means of a light pulse of a short duration and the fluorescence is not detected until a certain period of time has elapsed from the excitation pulse.
  • 4,374,120 comprises a lanthanide chelate formed by a lanthanide and an aromatic ⁇ -diketone, the lanthanide being bound to antigen, hapten, antibody, nucleic acid or polynucleotide via an EDTA-analogue so that a fluorescent lanthanide complex is formed.
  • the fluorescence decay time of the label is long, 50 - 1000 ⁇ sec, which makes it most suitable for the time-resolved detection principle.
  • the fluorescence from the label can either be measured when the marker is bound to antigen, hapten, antibody, nucleic acid or polynucleotide, or the lanthanide can under suitably chosen circumstances be released from these by dissociating the bond between the lanthanide and the EDTA-analogue, the fluorescence being caused in solution in the presence of an aromatic ⁇ -diketone, a synergistic compound and a detergent which together with the lanthanide form a micellar system having a fluorescence which is characteristic of the lanthanide (European Patent Application No. 82850077.7).
  • the lanthanide should form a fluorescent chelate and on the other hand it should be bound to a bioorganic molecule, which is an antigen, a hapten, an antibody, a nucleic acid or a polynucleotide, in order to be usable as a label in biospecific affinity reactions.
  • the primary ligand only acts as an intermediary for the binding of the lanthanide to the bio-organic molecule.
  • the EDTA analogue mentioned earlier (U.S. Patent No. 4,374,120) follows the latter principle.
  • Aminophenyl-EDTA-Eu can e.g. be diazotated and thereafter be coupled to tyrosine or histidine residues in a protein.
  • the synthesized protein-EDTA-Eu complex gives, however, upon excitation a very low lanthanide fluorescence of a long decay time, since the primary ligand does not absorb and transfer the necessary excitation energy to the lanthanide.
  • the ligand functions excellently in biospecific affinity reactions according to the principles which are described in the U.S. Patent No. 4,374,120 and the European Patent Application No. 82850077.7.
  • Ethylenediamine tetraacetic acid is a well known and commonly used compound, which under suitable conditions forms stable chelates with a large number of metal ions (see Ringbom (1964) Kompleksometrisk analys) .
  • the chelate forming characteristics of the molecule can be utilized to bind e.g. lanthanides to bio-organic molecules for use in bio-specific affinity reactions, if a covalent coupling of the ligand to the bio-organic molecule can be carried out. This has been done by e.g.
  • an aminophenyl-EDTA derivative can be synthesized which can be used to bind the EDTA structure to the bioorganic molecule (see Sundberg et al, U.S. Patent 3,994,966).
  • EDTA diethylenetriaminepentaacetic acid
  • DTPA diethylenetriaminepentaacetic acid
  • DTPA has also been coupled to protein by the use of a cyclic anhydride of the molecule, four carboxyl groups then remaining for chelating (see: Hnatowich et al. (1982) Int. J. Appl. Radiat. Isot. 33, 327-332).
  • the object of the present invention is to provide a chelating compound, which strongly chelates i.a. lanthanides, but which also comprises active functional groups which makes it possible to bind the metal chelate to a bio-organic molecule comprising e.g. hapten, antigen, nucleic acid or antibody.
  • a chelating compound which strongly chelates i.a. lanthanides, but which also comprises active functional groups which makes it possible to bind the metal chelate to a bio-organic molecule comprising e.g. hapten, antigen, nucleic acid or antibody.
  • the drawing shows a typical AFP determination with duplicate standards for seven different concentrations.
  • R is a 2 to 8 atoms long covalent bridge comprising alkylene groups
  • Y comprises carboxylic or phosphonic acid and the number varies depending on n and m.
  • X is an active functional group which permits coupling to a bio-organic molecule and comprises e.g. an aromatic ring comprising a NH 2 , OH, COOH or NCS group; n and m are 0 or 1.
  • X can change place with one of the Y functions in the molecule.
  • the chelating compounds can be synthesized in the following ways:
  • Step 1 2.0 g diethylenetriamine is dissolved in 25 ml toluene and 1.0 g 4-nitrobenzylbromide dissolved in 25 ml toluene is added. The reaction mixture is stirred for 3 hours at room temperature. The precipitate is filtrated and the toluene phase is extracted with water. The water phase is extracted with chloroform which is evaporated to dryness. The final product consists of a yellow syrup (0.85 g, 77% yield).
  • Step 2 The mixture of N 1 - and N 2 - (4-nitrobenzyl)-diethylenetriamine is dissolved in water.
  • the water solution is made alkaline (pH 9-11) with 7 M KOH solution and is heated during stirring to 50°C.
  • a water solution of bromoacetic acid (2.5 g) is added slowly and pH is kept between 9-11 by means of the KOH solution.
  • the stirring and the heating are continued for at least 4 hours, KOH solution being added now and then to keep pH in between 9 and 11.
  • the reaction mixture is acidified (pH about 1) , the insignificant precipitate appearing on the cooling, then being filtrated away.
  • the solution is evaporated to a smaller volume, a salt then beinq precipitated, the precipitation of which is facilitated by adding acetone.
  • the solution is evaporated to dryness and an impure raw product is obtained (3.1 g, containing about 50% of the desired compound).
  • the product is purified by.means of preparative liquid chromatography (Waters PrepPAK - 500/C 18 , with H 2 O) , then also the different isomers being separated from each other.
  • TLC silica gel; acetonitrile/water 4:1; R f for N 1 compound as Eu 3+ chelate 0.25 and N 2 compound 0.30.
  • Step 4 2.3 g of N 2 - (4-aminobenzyl)-diethylenetriamine-N 1 , N 1 , N 3 , N 3 - tetraacetic acid is dissolved in about 15 ml of water and the solution is added to a reagent mixture containing 1.7 g of thiophosgene, 15 ml of chloroform and 1 g of sodium hydrogen carbonate. The reaction mixture is strongly stirred for about 20 minutes in room temperature. The phases are separated and the water phase is washed with chloroform (3 x 5 ml) , it then being evaporated to dryness and the obtained product is washed with ethanol. Final product 2.2 g, yield 83%.
  • TLC Silica gel; acetonitrile/water 4 :1; R 1 f for N and
  • the other compounds mentioned can be synthesized in a corresponding way starting from the corresponding polyalkylene-polyamine.
  • the carboxylic groups can also be replaced by phosphonic acid (K. MOEDRITZER -R.R. IRANI. J.Org.Chem. 31, 1603 (1966).
  • the applicability of the invention is illustrated below by means of a non-limiting example of execution.
  • Example 1 Determination of alphaphetoprotein (AFP).
  • p-ITC-B-DTTA-Eu was added to an anti-AFP solution (0.2 mg in 200 ⁇ l PBS) at 0°C and the pH of the solution was adjusted to 9.5 by means of 10 ⁇ l of 1 M Na 2 CO 3 .
  • the molar ratio between chelate and antibody was 60:1.
  • the reaction mixture was incubated over night, whereupon the antibody conjugate was purified from free unreacted label by means of gel filtration on a Sephadex G-50 column (Pharmacia). The degree of conjugation was determined by measuring the europium fluorescence and it was found to be about 7 Eu/IgG. Immunoas say
  • Polystyrene tubes were coated with anti-AFP by incubating the tubes over night at room temperature with 250 ⁇ l of a solution containing 1 ⁇ g of anti-AFP in 50 mM K 2 HPO 4 + 9 g/l of NaCl.
  • the tube surface was saturated with 250 ⁇ l of a solution containing 0.5% of BSA in Tris-HCl buffer, pH 7.70 (2 hours at room temperature). After washing the tubes were used for the immunoassay.
  • 25 ⁇ l serum samplesor corresponding standards were incubated for 1 hour in the coated tubes together with 50 ng of europium labelled anti-AFP which had been added in 225 ⁇ l of Tris-HCl buffer, pH 7.7 containing 0.9% NaCl, 0.05% NaN 3 , 0.5% BSA, 0.05% bovine gammaglobuline, 0.01% Tween-40 and 20 ⁇ M DTPA. After incubation the tubes were aspirated and washed three times with 2 ml of physiological sodium chloride solution containing 0.05% NaN 3 .
  • the solution dissociates europium from the chelate, a new fluorescent chelate thus being formed in the micellar phase, the amount of which is proportional to the time-resolved fluorescence signal obtained and the amount of europium released.

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Abstract

Compound having structure (I), where R is a direct chain or branched alkylene group comprising 2-8 carbon atoms; n and m are 0 or 1, Y is a carboxylic or phosphonic acid, and X is an active funcitional group which permits covalent coupling to a bio-organic molecule.

Description

Compound.
TECHNICAL FIELD
The present invention refers to a compound.
BACKGROUND ART
Immunoassay is a field in which the sensitivity of the analysis method often is of decisive importance as the amount of analyte in different biological liquids usually is very low. As a result of this radioisotopes have been widely used as labels in immunoassays despite the dis- advantages caused by their use. At the same time, however, a very intense research has been carried out with the aim of replacing the radioisotopes with labels giving at least the same or a higher sensitivity than the isotopes. Fluorescent molecules have in these connections been presented as one of the most potential alternatives to radioisotopes. Comprehensive surveys have recently been published, which give a good general view of fluoroimmunoanalytical determinations known at present (see Smith et al. (1981) Ann. Clin. Biochem. 18, 253-274, Ullman (1981) "Recent Advances in Fluorescence Immunoassay techniques").
The sensitivity of the fluorescent labels in immunoassay, in spite of the fact that it is theoretically very high, has been seriously limited by a high background fluorescence. Usually, it has been possible to reduce the background fluorescence, so that a desired sensitivity could be obtained. The above mentioned surveys also describe the limitations which have made the use of conventional fluorescent labels difficult in immunoassay of analytes which require a high sensitivity corresponding to that which can be obtained with radioisotopes.
The use of time-resolved fluorescence (see Soini et al (1979) Clin. Chem. 25, 353-361) makes it, however, possible to separate the specific fluorescence of the label from the disturbing, unspecific background fluorescence. The principle of the use of time-resolved fluorescence when following biospecific affinity reactions is described in the U.S. Patent No. 4,374,120 and the European patent application No. 82850077.7. In time-resolved fluorescence the fluorescent label is excited by means of a light pulse of a short duration and the fluorescence is not detected until a certain period of time has elapsed from the excitation pulse. During the time which passes between excitation and detection, the fluorescence from any interfering sources will decay, so that only the signal from the label usable for time-resolved fluorescence is detected. Such a label should have as high fluorescence as possible, a relatively long emission wave-length, a large Stoke's shift and a chemical structure which makes it possible to couple the label covalently to antigens, haptens, antibodies nucleic acids and polynucleotides. A fluorescence label, which fulfils the above mentioned requirements (U.S. Patent No. 4,374,120) comprises a lanthanide chelate formed by a lanthanide and an aromatic β-diketone, the lanthanide being bound to antigen, hapten, antibody, nucleic acid or polynucleotide via an EDTA-analogue so that a fluorescent lanthanide complex is formed. The fluorescence decay time of the label is long, 50 - 1000 μsec, which makes it most suitable for the time-resolved detection principle. The fluorescence from the label can either be measured when the marker is bound to antigen, hapten, antibody, nucleic acid or polynucleotide, or the lanthanide can under suitably chosen circumstances be released from these by dissociating the bond between the lanthanide and the EDTA-analogue, the fluorescence being caused in solution in the presence of an aromatic β-diketone, a synergistic compound and a detergent which together with the lanthanide form a micellar system having a fluorescence which is characteristic of the lanthanide (European Patent Application No. 82850077.7).
In the use of lanthanides as labels in biospecific affinity reactions two functions can in principle be distinguished. On the one hand, the lanthanide should form a fluorescent chelate and on the other hand it should be bound to a bioorganic molecule, which is an antigen, a hapten, an antibody, a nucleic acid or a polynucleotide, in order to be usable as a label in biospecific affinity reactions.
The prerequisites for the formation of a fluorescent lanthanide chelate are described in the European Patent Application No. 83850244.1. A specific controlled binding of a lanthanide to a bio-organic molecule has proved to be difficult even if a number of alternative solutions has been tested. In such a binding it is desirable that the lanthanide is bound to the bio-organic molecule with a very high affinity and that the binding is kinetically stable. The primary ligand which is covalently bound to the bioorganic molecule and which also chelates the lanthanide, can also absorb the excitation energy which is then transferred to the lanthanide according to the principles which are described in the European Patent Application No. 83850244.1, or alternatively the primary ligand only acts as an intermediary for the binding of the lanthanide to the bio-organic molecule. The EDTA analogue mentioned earlier (U.S. Patent No. 4,374,120) follows the latter principle. Aminophenyl-EDTA-Eu can e.g. be diazotated and thereafter be coupled to tyrosine or histidine residues in a protein. The synthesized protein-EDTA-Eu complex gives, however, upon excitation a very low lanthanide fluorescence of a long decay time, since the primary ligand does not absorb and transfer the necessary excitation energy to the lanthanide. In spite of this the ligand functions excellently in biospecific affinity reactions according to the principles which are described in the U.S. Patent No. 4,374,120 and the European Patent Application No. 82850077.7.
Ethylenediamine tetraacetic acid (EDTA) is a well known and commonly used compound, which under suitable conditions forms stable chelates with a large number of metal ions (see Ringbom (1964) Kompleksometrisk analys) . The chelate forming characteristics of the molecule can be utilized to bind e.g. lanthanides to bio-organic molecules for use in bio-specific affinity reactions, if a covalent coupling of the ligand to the bio-organic molecule can be carried out. This has been done by e.g. synthesizing an EDTA dianhydride, which is coupled to a suitable bio-organic molecule, a diaminetriacetic acid derivative of the molecule is, then, obtained when the fourth carboxyl group is used for the conjugation (see. Wieder et al, U.S. Patent 4,353,751).
Moreover, an aminophenyl-EDTA derivative can be synthesized which can be used to bind the EDTA structure to the bioorganic molecule (see Sundberg et al, U.S. Patent 3,994,966). There are, however, other compounds than EDTA. which form stable chelates with metal ions (see Ringbom (1964) Kompleksometrisk analys). One of them, diethylenetriaminepentaacetic acid (DTPA) has e.g. been used to bind radioisotopes in connection with the examination of kidney functions (see Klopper et al. (1972) J.Nucl. Med. 13, 107-110). DTPA has also been coupled to protein by the use of a cyclic anhydride of the molecule, four carboxyl groups then remaining for chelating (see: Hnatowich et al. (1982) Int. J. Appl. Radiat. Isot. 33, 327-332).
DISCLOSURE OF INVENTION
The object of the present invention is to provide a chelating compound, which strongly chelates i.a. lanthanides, but which also comprises active functional groups which makes it possible to bind the metal chelate to a bio-organic molecule comprising e.g. hapten, antigen, nucleic acid or antibody. The characteristic features of the invention are apparent from the claims attached to the specification.
BRIEF DESCRIPTION OF DRAWING
The drawing shows a typical AFP determination with duplicate standards for seven different concentrations. DETAILED DESCRIPTION
Compounds according to the invention can be derived starting from the following basic structure
Figure imgf000007_0001
in which R is a 2 to 8 atoms long covalent bridge comprising alkylene groups Y comprises carboxylic or phosphonic acid and the number varies depending on n and m. X is an active functional group which permits coupling to a bio-organic molecule and comprises e.g. an aromatic ring comprising a NH2, OH, COOH or NCS group; n and m are 0 or 1.
X can change place with one of the Y functions in the molecule.
The chelating compounds can be synthesized in the following ways:
Step 1: 2.0 g diethylenetriamine is dissolved in 25 ml toluene and 1.0 g 4-nitrobenzylbromide dissolved in 25 ml toluene is added. The reaction mixture is stirred for 3 hours at room temperature. The precipitate is filtrated and the toluene phase is extracted with water. The water phase is extracted with chloroform which is evaporated to dryness. The final product consists of a yellow syrup (0.85 g, 77% yield).
TLC: silica gel; ammonia ethanol 1:4; Rf for N1 compound 0.38 and for N2 compound 0.29. 1H-N.M.R. (CDCl3) : δ = 7.8 ( q, 4 H, ), 3.7 tor N2
Figure imgf000008_0001
and 3,9 for N1 compound (S, 2H, -N),
Figure imgf000008_0002
2,7 (m, 8H,-CH2CH2-)1-,6 (S, 2H-NH-&-NH2)
U.V. (H2O) : λ max = 273 nm
Step 2: The mixture of N1- and N2- (4-nitrobenzyl)-diethylenetriamine is dissolved in water. The water solution is made alkaline (pH 9-11) with 7 M KOH solution and is heated during stirring to 50°C. A water solution of bromoacetic acid (2.5 g) is added slowly and pH is kept between 9-11 by means of the KOH solution. After the addition the stirring and the heating (50°C) are continued for at least 4 hours, KOH solution being added now and then to keep pH in between 9 and 11. The reaction mixture is acidified (pH about 1) , the insignificant precipitate appearing on the cooling, then being filtrated away. The solution is evaporated to a smaller volume, a salt then beinq precipitated, the precipitation of which is facilitated by adding acetone. The solution is evaporated to dryness and an impure raw product is obtained (3.1 g, containing about 50% of the desired compound). The product is purified by.means of preparative liquid chromatography (Waters PrepPAK - 500/C18, with H2O) , then also the different isomers being separated from each other.
TLC: silica gel; acetonitrile/water 4:1; Rf for N1 compound
0.16 and for N2 compound 0.33. 13C-N.M.R. (D2O) : δ=52.4 & 52.6 (-CH2CH2-) , 57.8 (-CH2COOH)
59.9
Figure imgf000008_0003
, 126.9, 134.3, 141.8 &
150.7
Figure imgf000008_0004
, 173.9 (-COOH) (for
N2 compound) U.V. (H2O) : λmax = 269 nm I.R. (KBr) : Vmax = 1740, 1530, 1350 cm-1 Step 3: 2.0 g of a compound from the previous synthesis step is dissolved in 50 ml water and 0.2 g of palladium on activated carbon (5%) is added to the solution in a pressure reactor. The reactor is cooled to 0 - +5ºC and is washed with nitrogen gas and hydrogen gas. The reduction takes place at 0 - +5°C at about 1 MPa (pressure above atmospheric). The reaction is followed on thin layer plates (acetonitrile water 4:1), by means of a liquid chromatography (HPLC) or UV-spectrophotometry. Final product 1.7 g, yield 89%.
TLC: silica gel; acetonitrile/water 4:1; Rf for N1 compound as Eu3+ chelate 0.25 and N2 compound 0.30.
13C-N.M.R. (D20) : δ =150.9, 140.9, 121.7 & 120.0
Figure imgf000009_0001
183 (-COOH) (for N compound as Eu3+ chelate) U.V. (H2O) : λ max = 284 & 238 nm (~1:8) I.R. (KBr) : V max = 1580-1640, 1400 cm-1
Step 4: 2.3 g of N2 - (4-aminobenzyl)-diethylenetriamine-N1, N1, N3 , N3 - tetraacetic acid is dissolved in about 15 ml of water and the solution is added to a reagent mixture containing 1.7 g of thiophosgene, 15 ml of chloroform and 1 g of sodium hydrogen carbonate. The reaction mixture is strongly stirred for about 20 minutes in room temperature. The phases are separated and the water phase is washed with chloroform (3 x 5 ml) , it then being evaporated to dryness and the obtained product is washed with ethanol. Final product 2.2 g, yield 83%.
TLC: Silica gel; acetonitrile/water 4 :1; R 1 f for N and
N2 compounds 0.45
U.V. (H20) : λ max = 268 x 280 nm ( ~1:1) I.R. (KBr): V max = 2000-2200 cm-1 Reaction scheme: Step 1:
Figure imgf000010_0001
Step 2:
Figure imgf000010_0002
(The isomers are separated by HPLC) . Step 3:
Figure imgf000011_0001
Pd/C (5%) Corresponding aminoderivative
Step 4:
Figure imgf000011_0002
Cl2C = S
Corresponding isothiocyanato derivative.
The other compounds mentioned can be synthesized in a corresponding way starting from the corresponding polyalkylene-polyamine. The carboxylic groups can also be replaced by phosphonic acid (K. MOEDRITZER -R.R. IRANI. J.Org.Chem. 31, 1603 (1966). The applicability of the invention is illustrated below by means of a non-limiting example of execution.
Example 1. Determination of alphaphetoprotein (AFP). The chelating compound N2 - (p - isothiocyanato benzyl)diethylenetriamine - N1, N1 , N3, N3 - tetraacetic acid
(p - ITC - B - DTTA) is used to chelate europium and to bind the europium chelate to a monoclonal anti-AFP antibody. The europium labelled antibody was used in the immunoassay of AFP.
Labelling the antibody with europium
p-ITC-B-DTTA-Eu was added to an anti-AFP solution (0.2 mg in 200 μl PBS) at 0°C and the pH of the solution was adjusted to 9.5 by means of 10 μl of 1 M Na2CO3. The molar ratio between chelate and antibody was 60:1. The reaction mixture was incubated over night, whereupon the antibody conjugate was purified from free unreacted label by means of gel filtration on a Sephadex G-50 column (Pharmacia). The degree of conjugation was determined by measuring the europium fluorescence and it was found to be about 7 Eu/IgG. Immunoas say
Polystyrene tubes were coated with anti-AFP by incubating the tubes over night at room temperature with 250 μl of a solution containing 1 μg of anti-AFP in 50 mM K2HPO4 + 9 g/l of NaCl. The tube surface was saturated with 250 μl of a solution containing 0.5% of BSA in Tris-HCl buffer, pH 7.70 (2 hours at room temperature). After washing the tubes were used for the immunoassay.
25 μl serum samplesor corresponding standards were incubated for 1 hour in the coated tubes together with 50 ng of europium labelled anti-AFP which had been added in 225 μl of Tris-HCl buffer, pH 7.7 containing 0.9% NaCl, 0.05% NaN3, 0.5% BSA, 0.05% bovine gammaglobuline, 0.01% Tween-40 and 20 μM DTPA. After incubation the tubes were aspirated and washed three times with 2 ml of physiological sodium chloride solution containing 0.05% NaN3.
Quantitation of europium by means of time-resolved fluorescence.
The amount of europium, which via the chelate and the labelled antibody has been bound in the immunometric analysis to the surface of the tube, was quantitated by adding 0.5 ml enhancement solution (15 μM β-naphthoyltrifluoroacetone, 50 μM. trioctylphosphine oxide, 0.1 % of Triton X-100 in phthalate-acetate-buffer pH 3.2). The solution dissociates europium from the chelate, a new fluorescent chelate thus being formed in the micellar phase, the amount of which is proportional to the time-resolved fluorescence signal obtained and the amount of europium released.
Result
The result from a typical AFP determination with duplicate standards for seven different concentrations is shown in Table 1 and on the attached drawing.

Claims

Claims
1. Compound having the following structure:
, where
Figure imgf000013_0001
- R is a direct chain or branched alkylene group comprising 2-8 carbon atoms,
- n and m are 0 or 1,
- Y is a carboxylic or phosphonic acid, and
- X is an active functional group which permits covalent coupling to a bio-organic molecule.
2. Compound according to claim 1, wherein the active functional group comprises an alkylene group with
2-8 carbon atoms, a phenyl ring or an aromatic hetero- cyclic ring which has a NH2-, HO-, -COOH, isothiocyanate or isocyanate group as a substituent.
3. Compound according to claim 1, wherein X can change place with one of the Y-functions in the molecule.
4. Compound according to claim 1, wherein
- R is an ethylene group,
- n = m = O,
- Y is carboxylic acid, and
- X is either a p-aminophenyl or a p-isothiocyanatophenyl group.
PCT/SE1984/000089 1983-03-15 1984-03-13 Compound WO1984003698A1 (en)

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WO1987004523A1 (en) * 1986-01-23 1987-07-30 Robert Stephen Davidson Binding assay and assay reagent
US5811526A (en) * 1986-01-23 1998-09-22 Alusuisse Holdings A.G. Binding assay and assay reagent
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EP0328529A1 (en) * 1986-09-05 1989-08-23 Otto A Gansow Method for preparing polysubstituted diethylenetriaminepentaacetic acids.
EP0328529A4 (en) * 1986-09-05 1991-03-20 Otto A. Gansow Backbone polysubstituted chelates for forming a metal chelate-protein conjugate
EP0484989A1 (en) * 1986-09-05 1992-05-13 GANSOW, Otto A. Polysubstituted diethylenetriamine chelates for forming a metal-chelate-protein conjugate
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US5256535A (en) * 1986-10-17 1993-10-26 Jyrki Ylikoski Hybridization assay and means to be used in the assay
WO1989012631A1 (en) * 1988-06-24 1989-12-28 The Dow Chemical Company Macrocyclic bifunctional chelants, complexes thereof and their antibody conjugates
EP0353450A1 (en) * 1988-06-24 1990-02-07 The Dow Chemical Company Macrocyclic bifunctional chelants, complexes thereof and their antibody conjugates
US5435990A (en) * 1988-06-24 1995-07-25 The Dow Chemical Company Macrocyclic congugates and their use as diagnostic and therapeutic agents
US5756065A (en) * 1988-06-24 1998-05-26 The Dow Chemical Company Macrocyclic tetraazacyclododecane conjugates and their use as diagnostic and therapeutic agents
US5652361A (en) * 1988-06-24 1997-07-29 The Dow Chemical Company Macrocyclic ligands and complexes
EP0570022A2 (en) 1988-10-31 1993-11-18 The Dow Chemical Company Chelants possessing ortho ligating functionality and complexes thereof
EP0374947A1 (en) * 1988-12-23 1990-06-27 The Dow Chemical Company Process for preparing isothiocyanato functionalized metal complexes
WO1990014881A2 (en) * 1989-05-26 1990-12-13 Akzo N.V. Chelating agents for attaching metal ions to proteins
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EP0540975A1 (en) * 1991-11-06 1993-05-12 Bayer Ag Diethylenetriamine derivatives and their use for diagnostic and therapeutic purposes
WO1995018832A1 (en) * 1994-01-07 1995-07-13 Akzo Nobel N.V. New polyaminocarboxylate chelators
WO2001036973A2 (en) * 1999-11-16 2001-05-25 Otto Wolfbeis Method for solubilising optical markers
WO2001036973A3 (en) * 1999-11-16 2002-02-14 Otto Wolfbeis Method for solubilising optical markers
US11845976B2 (en) 2019-08-27 2023-12-19 SeLux Diagnostics, Inc. Systems and methods for performing antimicrobial susceptibility testing

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