WO1983000741A1 - Cup for thin layer chromatography - Google Patents

Cup for thin layer chromatography Download PDF

Info

Publication number
WO1983000741A1
WO1983000741A1 PCT/EP1982/000181 EP8200181W WO8300741A1 WO 1983000741 A1 WO1983000741 A1 WO 1983000741A1 EP 8200181 W EP8200181 W EP 8200181W WO 8300741 A1 WO8300741 A1 WO 8300741A1
Authority
WO
WIPO (PCT)
Prior art keywords
carrier
layer chromatography
thin layer
glass
cup
Prior art date
Application number
PCT/EP1982/000181
Other languages
German (de)
French (fr)
Inventor
Gmbh Desaga
Original Assignee
Berkhan, Ernst
Detemple, Manfred
RIEGE, Günter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkhan, Ernst, Detemple, Manfred, RIEGE, Günter filed Critical Berkhan, Ernst
Publication of WO1983000741A1 publication Critical patent/WO1983000741A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography

Definitions

  • the present invention relates to a dip chamber for thin layer chromatography.
  • Detection reagents are often used in thin-layer or paper chromatography. DTese react with the substances to be detected and form reaction products which can be detected with simpler means and / or with higher sensitivity than the starting substance.
  • the detection reagents are often sprayed on as a liquid mist in order to avoid flow processes in the carrier which change the position and / or shape of the substance stains.
  • Other ways of supplying the detection reagents are gassing with reagent vapors or immersion in a reagent solution. Only rarely can the reagent be added to the carrier or the substance before the substance to be detected is applied.
  • Spraying the carrier with a reagent solution is difficult to do uniformly, since the individual droplets of the spray mist form only individual, irregularly distributed spots on the carrier if the density is too low, and if the density is too high, on the other hand, there is the risk of flow processes in or on the carrier Carrier exists.
  • Elaborate devices for the controlled relative movement of the spray can and carrier with respect to one another were developed in order to achieve the uniformity of spraying necessary for quantitative evaluation of the reaction products.
  • complex devices are required in order to safely exclude the health hazard to persons from the spray mist and / or gases or vapors emanating therefrom.
  • a horizontal position of the carrier in the reagent is possible in flat dishes with little reagent requirement, but flow processes in or on the carrier cannot be avoided when immersed and removed.
  • the aim of the present invention is a diving chamber for thin-layer chromatography, which does not have the disadvantages described above of the diving chambers made of metal or plastic.
  • This object is achieved according to the invention by using a narrow, vertical chamber made of glass.
  • Glass is used as a material according to the invention, since only glass is used against the commonly used organic ones
  • Solvent, acid, bases and / or oxidizing reagents is resistant.
  • the narrow glass chamber can also be used with very aggressive means, e.g. Chromium sulfuric acid, clean from reagent lakes.
  • immersion chambers of this type have not yet been manufactured, a suitable manufacturing technique first had to be developed. It was found that the inventive modern immersion chambers can be produced particularly successfully from two flat glass plates which are fused together on three edges.
  • the inside thickness of the chamber is typically 5-10 mm, while the inside width is chosen to be larger than the width of the carrier.
  • the inside height of the chamber can be 10-20 mm smaller than the height of the carrier so that the carrier protrudes from the top of the chamber for easier handling. But it is also possible holder for the support, the narrow glass chamber is immersed with the aid of the carrier in full in ⁇ .
  • FIG. 1 shows an exemplary embodiment of the invention: the immersion chamber consists of two glass plates 1 and 2 at a distance of approximately 5 mm, which are fused together on three edges.

Abstract

The cup intended to receive the reactants used in thin layer chromatography or in chromatography on paper is made of glass, in opposition to conventional cups. It is provided by fusion welding the three sides of two glass plates.

Description

TAUCHKAMMER FÜR DIE DÜNNSCHICHT-CHROMATOGRAPHIE DIVING CHAMBER FOR THIN-LAYER CHROMATOGRAPHY
Die vorliegende Erfindung betrifft eine Tauchkammer für die Dünnschicht-Chromatographie.The present invention relates to a dip chamber for thin layer chromatography.
Zum Nachweis von Substanzen auf festen Trägern, z.B. in der Dünnschicht- oder Papier-Chromatographie, werden oft Nachweis- Reagenzien eingesetzt. DTese reagieren mit den nachzuweisen¬ den Substanzen und bilden Reaktionsprodukte, die mit ein¬ facheren Mitteln und/oder mit höherer Empfindlichkeit als die Ausgangssubstanz nachgewiesen werden können. Die Nach- weisreagenzien werden oft als Flüssigkeitsnebel aufgesprüht, um Fließvorgänge im Träger zu vermeiden, durch die die Posi¬ tion und/oder Form der Substanzflecke verändert werden. Andere Möglichkeiten der Zuführung der Nachweisreagenzien sind die Begasung mit Reagenzdämpfen oder das Eintauchen in eine Rea- genzlösung. Nur selten kann das Reagenz vor dem Aufbringen der nachzuweisenden Substanz dem Träger oder der Substanz zu¬ gefügt werden.For the detection of substances on solid supports, e.g. Detection reagents are often used in thin-layer or paper chromatography. DTese react with the substances to be detected and form reaction products which can be detected with simpler means and / or with higher sensitivity than the starting substance. The detection reagents are often sprayed on as a liquid mist in order to avoid flow processes in the carrier which change the position and / or shape of the substance stains. Other ways of supplying the detection reagents are gassing with reagent vapors or immersion in a reagent solution. Only rarely can the reagent be added to the carrier or the substance before the substance to be detected is applied.
Das Besprühen des Trägers mit einer Reagenzlösung kann nur schwer gleichmäßig erfolgen, da die einzelnen Tröpfchen des Sprühnebels bei zu geringer Dichte auf dem Träger nur einzel¬ ne, unregelmäßig verteilte Flecken bilden, bei zu hoher Dichte dagegen die Gefahr von Fließvorgängen in bzw. auf dem Träger besteht. Aufwendige Geräte zur kontrollierten Relativ¬ bewegung von Sprühdose und Träger zueinander wurden ent- wickelt, um die für quantitative Auswertung der Reaktions¬ produkte notwendige Gleichmäßigkeit der Besprühung zu er¬ reichen. Darüberhinaus sind aufwendige Vorrichtungen erforder¬ lich, um die gesundheitliche Gefährdung von Personen durch den Sprühnebel und/oder davon ausgehende Gase oder Dämpfe mit Sicherheit auszuschließen. Diese Nachteile werden vermieden durch Eintauchen der Träger in die Reagenzlösungen. Eine gleichmäßige und gefahrlose Einwirkung der Reagenzien auf den Träger ist damit sicherge¬ stellt. Beim Eintauchen der Träger in vertikaler Lage in die üblichen Glasgefäße wird jedoch eine sehr große Menge der teuren und/oder nur kurze Zeit haltbaren Reagenzien benötigt, da Glasgefäße" in den notwendigen Abmessungen für die üblich¬ erweise verwendeten Träger mit geringer lichter Weite senk¬ recht zur Trägerplatte bisher nicht bekannt waren. Man hat sich deshalb damit beholfen, solche Tauchkammern (Küvetten) aus anderen Materialien, --wie Edelstahl oder Kunststoffen, herzustellen. Da. diese jedoch von den Reagenzien angegrif¬ fen werden, haben sie nur eine kurze Gebrauchsdauer.Spraying the carrier with a reagent solution is difficult to do uniformly, since the individual droplets of the spray mist form only individual, irregularly distributed spots on the carrier if the density is too low, and if the density is too high, on the other hand, there is the risk of flow processes in or on the carrier Carrier exists. Elaborate devices for the controlled relative movement of the spray can and carrier with respect to one another were developed in order to achieve the uniformity of spraying necessary for quantitative evaluation of the reaction products. In addition, complex devices are required in order to safely exclude the health hazard to persons from the spray mist and / or gases or vapors emanating therefrom. These disadvantages are avoided by immersing the carrier in the reagent solutions. This ensures a uniform and safe effect of the reagents on the carrier. When the supports are immersed in the vertical position in the usual glass vessels, however, a very large amount of the expensive and / or only short-lived reagents is required, since glass vessels " in the necessary dimensions for the commonly used supports with a narrow inside diameter are vertical So far, it has been used to manufacture such immersion chambers (cuvettes) from other materials, such as stainless steel or plastics, but since they are attacked by the reagents, they have only a short service life .
Eine horizontale Lage der Träger im Reagenz ist in flachen Schalen bei geringem Reagenzbedarf zwar möglich, jedoch sind beim Eintauchen und Herausnehmen Fließvorgänge in oder auf dem Träger nicht zu vermeiden.A horizontal position of the carrier in the reagent is possible in flat dishes with little reagent requirement, but flow processes in or on the carrier cannot be avoided when immersed and removed.
Ziel der vorliegenden Erfindung ist eine Tauchkammer für die Dünnschicht-Chromatographie, die die geschilderten Nachtei- le der aus Metall oder Kunststoff bestehenden Tauchkammern nicht aufweist. Dieses Ziel wird gemäß der Erfindung da¬ durch erreicht, daß eine schmale, senkrecht stehende Kammer aus Glas verwendet wird.The aim of the present invention is a diving chamber for thin-layer chromatography, which does not have the disadvantages described above of the diving chambers made of metal or plastic. This object is achieved according to the invention by using a narrow, vertical chamber made of glass.
Glas wird erfindungsgemäß als Werkstoff verwendet, da nur Glas gegen die üblicherweise verwendeten organischenGlass is used as a material according to the invention, since only glass is used against the commonly used organic ones
Lösungsmittel, Säure, Basen und/oder oxidierenden Reganzien resistent ist. Darüberhinaus läßt sich die schmale Glas¬ kammer auch mit sehr aggressiven Mitteln, z.B. Chromschwe- felsäure, von Reagenzreseen reinigen.Solvent, acid, bases and / or oxidizing reagents is resistant. In addition, the narrow glass chamber can also be used with very aggressive means, e.g. Chromium sulfuric acid, clean from reagent lakes.
Da Tauchkammern dieser Bauart bisher noch nicht gefertigt wurden, mußte erst eine geeignete Herstellungstechnik ent¬ wickelt werden. Es wurde gefunden, daß die erfindungsge- mäßen Tauchkammern besonders erfolgreich aus zwei planen Glasplatten hergestellt werden können, die an drei Rändern miteinander verschmolzen werden.Since immersion chambers of this type have not yet been manufactured, a suitable manufacturing technique first had to be developed. It was found that the inventive modern immersion chambers can be produced particularly successfully from two flat glass plates which are fused together on three edges.
Die lichte Dicke der Kammer beträgt typischerweise 5-10 mm, während die lichte Breite größer als die Breite des Trägers gewählt wird. Die lichte Höhe der Kammer kann 10-20 mm kleiner als die Höhe des Trägers sein, damit der Träger zur leichteren Handhabung oben aus der Kammer ragt. Es sind aber auch Halter für den Träger möglich, mit deren Hilfe der Träger in voller Höhe in ^die schmale Glaskammer eingetaucht wird.The inside thickness of the chamber is typically 5-10 mm, while the inside width is chosen to be larger than the width of the carrier. The inside height of the chamber can be 10-20 mm smaller than the height of the carrier so that the carrier protrudes from the top of the chamber for easier handling. But it is also possible holder for the support, the narrow glass chamber is immersed with the aid of the carrier in full in ^.
Figur 1 zeigt eine beispielhafte Ausführungsform der Er¬ findung: Die Tauchkammer besteht aus zwei Glasplatten 1 und 2 in etwa 5 mm Abstand, die an drei Rändern miteinander verschmolzen sind.FIG. 1 shows an exemplary embodiment of the invention: the immersion chamber consists of two glass plates 1 and 2 at a distance of approximately 5 mm, which are fused together on three edges.
< y V/IFO <y V / IFO

Claims

Patentansprüchei Claims i
1. Küvette mit einer lichten Breite von mehr als 100 mm, einer lichten Höhe von mehr als 50 mm und einer lichten Dicke von weniger als 20 mm, dadurch gekennzeichnet, daß sie ausschließlich aus Glas besteht.1. Cell with a clear width of more than 100 mm, a clear height of more than 50 mm and a clear thickness of less than 20 mm, characterized in that it consists exclusively of glass.
2. Küvette nach Anspruch 1, dadurch gekennzeichnet, daß sie aus 2 planen Glasplatten besteht, die an drei Seiten miteinander verschmolzen sind. 2. Cuvette according to claim 1, characterized in that it consists of 2 flat glass plates which are fused together on three sides.
PCT/EP1982/000181 1981-08-27 1982-08-26 Cup for thin layer chromatography WO1983000741A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE8125003 1981-08-27
DEG8125003.7U810827 1981-08-27

Publications (1)

Publication Number Publication Date
WO1983000741A1 true WO1983000741A1 (en) 1983-03-03

Family

ID=6730687

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1982/000181 WO1983000741A1 (en) 1981-08-27 1982-08-26 Cup for thin layer chromatography

Country Status (3)

Country Link
EP (1) EP0086815A1 (en)
CH (1) CH656467A5 (en)
WO (1) WO1983000741A1 (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB929578A (en) * 1960-07-05 1963-06-26 Shandon Scient Company Ltd Improvements in paper-chromatography apparatus
FR1469350A (en) * 1966-02-14 1967-02-10 Euratom Development facility for thin-layer chromatography plates
US3501008A (en) * 1968-03-04 1970-03-17 Ceskoslovenska Akademie Ved Developing chamber for thin layer chromatograms
US3547023A (en) * 1967-10-09 1970-12-15 Eastman Kodak Co Chromatographic chamber
FR2068892A5 (en) * 1969-09-05 1971-09-03 Tlc Corp
FR2204305A5 (en) * 1972-10-19 1974-05-17 Takeshita Yuko
FR2221164A1 (en) * 1973-03-16 1974-10-11 Regis Chem Co
EP0014956A1 (en) * 1979-02-20 1980-09-03 Hoechst Aktiengesellschaft Microchromatographic system for checking the intake of medicines via urine samples

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB929578A (en) * 1960-07-05 1963-06-26 Shandon Scient Company Ltd Improvements in paper-chromatography apparatus
FR1469350A (en) * 1966-02-14 1967-02-10 Euratom Development facility for thin-layer chromatography plates
US3547023A (en) * 1967-10-09 1970-12-15 Eastman Kodak Co Chromatographic chamber
US3501008A (en) * 1968-03-04 1970-03-17 Ceskoslovenska Akademie Ved Developing chamber for thin layer chromatograms
FR2068892A5 (en) * 1969-09-05 1971-09-03 Tlc Corp
FR2204305A5 (en) * 1972-10-19 1974-05-17 Takeshita Yuko
FR2221164A1 (en) * 1973-03-16 1974-10-11 Regis Chem Co
EP0014956A1 (en) * 1979-02-20 1980-09-03 Hoechst Aktiengesellschaft Microchromatographic system for checking the intake of medicines via urine samples

Also Published As

Publication number Publication date
CH656467A5 (en) 1986-06-30
EP0086815A1 (en) 1983-08-31

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