WO1981001746A1 - Feuille chromatographique de couche pour appareil chromatographique a couche pressurisee - Google Patents

Feuille chromatographique de couche pour appareil chromatographique a couche pressurisee Download PDF

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Publication number
WO1981001746A1
WO1981001746A1 PCT/HU1980/000006 HU8000006W WO8101746A1 WO 1981001746 A1 WO1981001746 A1 WO 1981001746A1 HU 8000006 W HU8000006 W HU 8000006W WO 8101746 A1 WO8101746 A1 WO 8101746A1
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WO
WIPO (PCT)
Prior art keywords
layer
sheet
sorbent
edges
solvent
Prior art date
Application number
PCT/HU1980/000006
Other languages
English (en)
Inventor
E Tyihak
E Mincsovics
P Tetenyi
I Lak
H Kalasz
S Zoltan
J Nagy
J Knoll
Original Assignee
Mueszeripari Muevek Lab
E Tyihak
E Mincsovics
P Tetenyi
I Lak
H Kalasz
S Zoltan
J Nagy
J Knoll
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mueszeripari Muevek Lab, E Tyihak, E Mincsovics, P Tetenyi, I Lak, H Kalasz, S Zoltan, J Nagy, J Knoll filed Critical Mueszeripari Muevek Lab
Priority to DE8181900050T priority Critical patent/DE3071215D1/de
Publication of WO1981001746A1 publication Critical patent/WO1981001746A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/94—Development
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N2030/906—Plate chromatography, e.g. thin layer or paper chromatography pressurised fluid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24273—Structurally defined web or sheet [e.g., overall dimension, etc.] including aperture
    • Y10T428/24298—Noncircular aperture [e.g., slit, diamond, rectangular, etc.]
    • Y10T428/24314—Slit or elongated
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24273—Structurally defined web or sheet [e.g., overall dimension, etc.] including aperture
    • Y10T428/24322—Composite web or sheet
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24273—Structurally defined web or sheet [e.g., overall dimension, etc.] including aperture
    • Y10T428/24322—Composite web or sheet
    • Y10T428/24331—Composite web or sheet including nonapertured component
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24479—Structurally defined web or sheet [e.g., overall dimension, etc.] including variation in thickness
    • Y10T428/24488—Differential nonuniformity at margin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24479—Structurally defined web or sheet [e.g., overall dimension, etc.] including variation in thickness
    • Y10T428/2457—Parallel ribs and/or grooves
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24479—Structurally defined web or sheet [e.g., overall dimension, etc.] including variation in thickness
    • Y10T428/24612—Composite web or sheet
    • Y10T428/2462—Composite web or sheet with partial filling of valleys on outer surface
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24777—Edge feature
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24777—Edge feature
    • Y10T428/24793—Comprising discontinuous or differential impregnation or bond
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24802—Discontinuous or differential coating, impregnation or bond [e.g., artwork, printing, retouched photograph, etc.]
    • Y10T428/24843—Discontinuous or differential coating, impregnation or bond [e.g., artwork, printing, retouched photograph, etc.] with heat sealable or heat releasable adhesive layer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/24—Structurally defined web or sheet [e.g., overall dimension, etc.]
    • Y10T428/24802—Discontinuous or differential coating, impregnation or bond [e.g., artwork, printing, retouched photograph, etc.]
    • Y10T428/24926—Discontinuous or differential coating, impregnation or bond [e.g., artwork, printing, retouched photograph, etc.] including ceramic, glass, porcelain or quartz layer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/25—Web or sheet containing structurally defined element or component and including a second component containing structurally defined particles
    • Y10T428/252—Glass or ceramic [i.e., fired or glazed clay, cement, etc.] [porcelain, quartz, etc.]

Definitions

  • the invention relates to specially prepared chromatographic sheet of layer favourably used in the pressurized layer-chromatographic apparatuses, for the development of linear chromatogram.
  • the layer chromatography as liquid chromatography of planar arrangement is a method whereby a mobile phase
  • solvent composite travels on the stationary phase (on solvent composite or sorbent) and carries one or several substances at varying speed. The most significant process of those, taking place meanwhile is the distribution of the travelling compounds between the two phases.
  • Substance of the pressurized layer chromatography is that the solvent system is flown on the sorbent layer with overpressure in a closed system.
  • the sorbent layer is complet.ely covered suitably with elastic foil, and which is pressed cushion -like to the sorbent layer v/ith the use of external pressure.
  • the cushion pressure (2-10 bar) shall always be higher than the flow pressure (1-8 bar) of the liquid composite.
  • pressurized ultramicron chamber a better separation can be attained within a very short time (1-10 minutes), than in the earlier known chromatographic methods of planar arrangement.
  • An apparatus was constructed for the development of both the concentric and linear chromatograms.
  • the invention is based on the recognition, that if the sorbent layer is suitably sealed at the edges of the sheet of layer, then the solvent can not escape in case of overpressure.
  • the invention is based on the recognition, that if the sorbent layer at and/or near the place of admitting the solvent composite is removed from the carrier plate in one or several thin zones, or thin baffle plate, or plates, or wires are placed directly in front of the place of admission, i.e. mini-solvent composite chamber are formed, then the solvent composite will run with straight front-line.
  • Sealing of the sorbent layer according to the invention is realized v/ith impregnation, or the sorbent layer is shrunk with the use of powdered glass, whereby varying shrinkage conditions are ensured at the edges of the layer, and in the chromatographic field, or one or several parallel grooves are formed in the carrier plate, and the sorbent layers are placed into these grooves, whereby the carrier plate itself seals the edges, or on case of using ceramic plate, its edges are glazed.
  • the impregnating or glazing materials shall be insoluble in the solvent used for the chromatography, they shall not be altered as a result of the overpressure of the solvent composite, and under the given temperature condition, and no chemical reaction should occur v/ith the sorbent layer or foil, sealing the sorbent layer from above.
  • Preferably paraffin or synthetic solutions, or suspensions, water-glass (sodium silicate) may be used as impregnating material.
  • the edges of the ceramic plates may be coated with white tinlead glaze. In case of shrinkage with pov/dered glass, varying quality of powdered glass is used on the edges, but the use of other additives on the edges is also suitable.
  • Width of the sealing layer may vary between 0.05 and 3 cm. Most favourable is the 1 cm or thinner layer. Length of the layer is the same as that of the chromatographic plate.
  • the straight front line of the developing solvent composite according to the invention is ensured in such a manner, that the sorbent layer is removed in a width of 0.1 - 0.5 mm in one or several zones at or near the place of admitting the solvent composite, as to have the zone of the carrier plate sorbent-free.
  • Maximum length of the so-formed grooves is the same as those of the sorbent layer retained after coating of the edges, but preferably shorter by 1-20mm on both sides (Fig. 1).
  • the grooves may be formed by displacement in relation to each other (Fig. 2).
  • Maximum depth of the grooves shall not exceed the thickness of the sorbent layer.
  • Their number may vary preferably between 1 and 4.
  • 0.05 - 1-0 m diameter wire or wires, or baffle plates can be arranged behind the place or places of sample application.
  • Maximum length of the baffle plates or wires after coating of the edges is the same as that of the retained sorbent layer, but preferably shorter by 1-20mm on both sides.
  • the baffle plates or wires can be displaced in relation to each other.
  • Their number is preferably 1-4
  • Preferably gapless synthetic plate is used fixed by glazing to the sheet of layer.
  • a so-called "concentration zone” can also be formed on the solvent admission part of the sheet of layer formed as described above, which means that a zone of another layer is applied on the sorbent layer (Fig. 3) -
  • the material of this layer may be for instance siliceous earth powdered glass, or synthetic powder, or cellite, i.e. rather carriers than ⁇ orbents used for separation (e.g. aluminium oxide, silica gel), but occasionally highly porous silica gel of wide pore range is useable against the low porosity, fine-grained layer.
  • the so-formed double layer facilitates the concentration of the sample.
  • Preferably fine-grained (0.5 - 10 /um), or normal (10-40 /urn) sheet of layer can be used for the chromatographic sheet of layer according to the invention.
  • Any sheet of layer available in the trade can be developed for the pressurized (sealed) layer-chromatography according to the invention.
  • the sorbent layer may be prepared domestically, too.
  • the developing solvent composite does not escape on the edges of the sheet of layer according to the invention in case of overpressure and it advances with straicght front.
  • Example 1 110 ml 0.85 %. watery solution of relatively highly polymerized homopolymeric polyacrylamide is added to 32 g silica gel powder of 4-8 /um grain size and 60 A inside diameter. After thorough mixing the 0.25 mm thick suspension is applied on degreased, 20 x 20 cm, 1,5mm thick glass panels according to the known method. The layers dried at room temperature are of good, mechanical strength even after activation.
  • Edges of the so-obtained and activated sheet of layers are impregnated in a width of 10 mm, by dipping on three sides into commercial paraffin melted at 130o C.
  • 0.2 mm diameter steel wire is placed in front of the solvent admission, as shown in Fig. 1.
  • authentic dye materials butter-yellow, Sudan G and indophenol
  • pressurized ultramicron chanber at 1.0 MPa cushion pressure, with 10 minute developing time, at a distance of 15 cm.
  • Example 3 The procedure is the same as in Example 1, but in place of 32 g silica gel powder, 30 g neutral aluminium oxide of 15-20 /um grain size is used, applied in a thickness of 0.3 mm.
  • Example 3
  • Edges (on three sides) of the so-obtained dried sheets of layer provided with control plate (foil) are impregnated by dipping into the 1.5 % watery solution of highly polymerized, non-ionogen polyacrylamide homopolymerism in a width of 5 mm and dried at room temperature.
  • Example 5 The procedure is the same as in Example 2, but two grooves each of 3 mm are formed in the layer for the solvent control (see schematic diagram in Fig. 2).
  • Example 5 The procedure is the same as in Example 2, but two grooves each of 3 mm are formed in the layer for the solvent control (see schematic diagram in Fig. 2).
  • Example 7 The procedure is the same as in Example 4, but 7 g cellite powder of 10-20 /um grain size is used instead of the 8 g siliceous earth. Example 7
  • Example 9 The procedure is the same as described in Example 3, except. that the two opposite long edges of the layer are impregnated still before drying of the sorbent layer by dipping into the 1.5 % watery solution of the highly polymerized non-ionogen polyacrylamide homopolymerism, and at the same time 1 mm thick groove is made in the centre of the layer at the intended place of solvent admission for the subsequent solvent control and two-directional development, then the layer is dried and used for development.
  • Example 10 The procedure is the same as described in Example 7 , except that the layer is made suitable for two-dimensional development by impregnating it on the 4th side and well after the one-dimensional development and the solvent control is solved as shown in Figure 4.
  • Example 10 The procedure is the same as described in Example 7 , except that the layer is made suitable for two-dimensional development by impregnating it on the 4th side and well after the one-dimensional development and the solvent control is solved as shown in Figure 4.
  • FIG. 1 Schematic diagram of finished silica gel layer with impregnated edge, including the operations.
  • a. silica gel zone impregnated with solid paraffic
  • b places of sample application
  • c place of solvent admission
  • Figure 2 Schematic diagram of silica gel layer with impreg nated edge. The solvent control is ensured with grooves.
  • a impregnated silica gel layer zone
  • b grooves
  • FIG 3 Silica gel layer with impregnated edge, provided with concentrating zone.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

Feuille chromatographique de couche pour appareil chromatographique a couche pressurisee, pour le developpement de chromatogrammes lineaires, caracterisee par le scellement de la couche sorbante au niveau des bords de la feuille de couche appliquee conventionnellement a une couche sorbante normale ou a grains fins, la couche sorbante est enlevee dans une ou plusieurs zones a proximite de l'admission du solvant ou un ou plusieurs deflecteurs ou fils sont disposes derriere l'endroit ou les endroits d'application des echantillons, et - si necessaire - elle est pourvue d'une zone de concentration formee de maniere conventionnelle a partir du sens d'admission du solvant. Le composite de solvant de developpement ne s'echappe pas aux bords de la feuille a couches dans le cas d'une surpression, et il avance suivant une ligne de front rectiligne.
PCT/HU1980/000006 1979-12-06 1980-12-05 Feuille chromatographique de couche pour appareil chromatographique a couche pressurisee WO1981001746A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE8181900050T DE3071215D1 (en) 1979-12-06 1980-12-05 Chromatographic sheet of layer for pressurized layer chromatographic apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU79CI1995A HU184065B (en) 1979-12-06 1979-12-06 Cromatographic layer sheet for a pressurized layer chromatographic equipment
HUCI1995 1979-12-06

Publications (1)

Publication Number Publication Date
WO1981001746A1 true WO1981001746A1 (fr) 1981-06-25

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ID=10994779

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU1980/000006 WO1981001746A1 (fr) 1979-12-06 1980-12-05 Feuille chromatographique de couche pour appareil chromatographique a couche pressurisee

Country Status (7)

Country Link
US (1) US4591524A (fr)
EP (1) EP0042393B1 (fr)
JP (1) JPS57500083A (fr)
DE (1) DE3071215D1 (fr)
HU (1) HU184065B (fr)
SU (1) SU1531866A3 (fr)
WO (1) WO1981001746A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0169951A1 (fr) * 1984-07-30 1986-02-05 Varex Corporation Système et appareil pour la chromatographie multidimensionnelle en temps réel
GB2166366A (en) * 1982-04-28 1986-05-08 Mueszeripari Muevek Lab Chromatographic sheet and/or a system for chromatographic sheets for overpressured multilayer chromatography
AU578078B2 (en) * 1984-03-16 1988-10-13 Dade Behring Marburg Gmbh Chromatographic devices having modified edges
CN103566912A (zh) * 2013-11-09 2014-02-12 四川师范大学 一种液相色谱固定相填料及其制备和应用

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883688A (en) * 1984-03-16 1989-11-28 Syntex (U.S.A) Inc. Method for producing chromatographic devices having modified edges
US5116495A (en) * 1987-09-11 1992-05-26 Ottosensors Corporation Capillary chromatography device
US6303029B1 (en) 1999-10-25 2001-10-16 Advanced Research And Technology Arrangement and method for performing chromatography
FR2843198B1 (fr) * 2002-08-02 2004-10-15 Bionisis Sa Dispositif de separation de constituants d'echantillons par chromatograhie liquide sous pression
US7736517B2 (en) * 2003-06-19 2010-06-15 Indiana University Research And Technology Corporation Method and apparatus for performing planar electrochromatography at elevated pressure
US8506802B1 (en) * 2010-01-25 2013-08-13 Gaston De Los Reyes Stackable planar adsorptive devices
CN102680638B (zh) * 2011-03-15 2014-04-23 上海高佳仪器科技有限公司 一种加压薄层色谱预制板的制造方法
DE102017118530A1 (de) 2017-08-14 2019-02-14 QC1 GmbH Platte für die Dünnschichtchromatographie
CN112997073A (zh) * 2018-10-03 2021-06-18 密歇根大学董事会 具有超薄紫外透射窗的集成式微型光致电离检测器
CN111366674A (zh) * 2020-04-13 2020-07-03 杭州旭昱科技有限公司 一种oplc加压薄层色谱仪多油缸加压装置

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US3791523A (en) * 1971-12-17 1974-02-12 Shionogi & Co Sintered chromatographic plate and method for producing the same
US3878100A (en) * 1973-02-01 1975-04-15 Marine Colloids Inc Separation medium
US4126554A (en) * 1977-09-02 1978-11-21 Analtech, Inc. Selectively marked thin-layer chromatographic plate
SU752165A1 (ru) * 1977-07-28 1980-07-30 Институт Нефтехимического Синтеза Им. А.В.Топчиева Ан Ссср Тонкослойна хроматографическа пластина

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CH364130A (de) * 1961-05-10 1962-08-31 Brenner Max Verfahren zur Durchführung von Dünnschicht-Chromatographie und Vorrichtung zur Durchführung des Verfahrens
US3482943A (en) * 1966-02-14 1969-12-09 Miles Lab Reagent deposition device
GB1183836A (en) * 1967-09-04 1970-03-11 May & Baker Ltd Improvements relating to Thin Layer Chromatography.
US3522792A (en) * 1967-11-14 1970-08-04 Us Agriculture Device for applying a coating of varying thickness
US3714035A (en) * 1971-05-19 1973-01-30 D Jones Method and apparatus for thin layer chromatography
US4065384A (en) * 1976-10-26 1977-12-27 University Of Illinois Foundation Graft thin layer chromatography

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3791523A (en) * 1971-12-17 1974-02-12 Shionogi & Co Sintered chromatographic plate and method for producing the same
US3878100A (en) * 1973-02-01 1975-04-15 Marine Colloids Inc Separation medium
SU752165A1 (ru) * 1977-07-28 1980-07-30 Институт Нефтехимического Синтеза Им. А.В.Топчиева Ан Ссср Тонкослойна хроматографическа пластина
US4126554A (en) * 1977-09-02 1978-11-21 Analtech, Inc. Selectively marked thin-layer chromatographic plate

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2166366A (en) * 1982-04-28 1986-05-08 Mueszeripari Muevek Lab Chromatographic sheet and/or a system for chromatographic sheets for overpressured multilayer chromatography
FR2572810A1 (fr) * 1982-04-28 1986-05-09 Mueszeripari Muevek Lab Feuille chromatographique et ensemble de feuilles chromatographiques pour chromatographie en couches multiples en surcompression
AU578078B2 (en) * 1984-03-16 1988-10-13 Dade Behring Marburg Gmbh Chromatographic devices having modified edges
EP0169951A1 (fr) * 1984-07-30 1986-02-05 Varex Corporation Système et appareil pour la chromatographie multidimensionnelle en temps réel
CN103566912A (zh) * 2013-11-09 2014-02-12 四川师范大学 一种液相色谱固定相填料及其制备和应用

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EP0042393A4 (fr) 1982-04-22
US4591524A (en) 1986-05-27
HU184065B (en) 1984-06-28
EP0042393A1 (fr) 1981-12-30
SU1531866A3 (ru) 1989-12-23
DE3071215D1 (en) 1985-12-05
EP0042393B1 (fr) 1985-10-30
JPS57500083A (fr) 1982-01-14

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