USRE32300E - Basic amino or ammonium antimicrobial agent-polyethylene glycol ester surfactant-betaine and/or amine oxide surfactant compositions and method of use thereof - Google Patents

Basic amino or ammonium antimicrobial agent-polyethylene glycol ester surfactant-betaine and/or amine oxide surfactant compositions and method of use thereof Download PDF

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USRE32300E
USRE32300E US06/752,332 US75233285A USRE32300E US RE32300 E USRE32300 E US RE32300E US 75233285 A US75233285 A US 75233285A US RE32300 E USRE32300 E US RE32300E
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alkyl
carbon atoms
hydroxyethyl
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skin
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William G. Gorman
Karl F. Popp
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STWB Inc
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    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/39Derivatives containing from 2 to 10 oxyalkylene groups
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/41Amines
    • A61K8/416Quaternary ammonium compounds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/43Guanidines
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4906Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
    • A61K8/4926Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/10Washing or bathing preparations
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/66Non-ionic compounds
    • C11D1/74Carboxylates or sulfonates esters of polyoxyalkylene glycols
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/66Non-ionic compounds
    • C11D1/75Amino oxides
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/88Ampholytes; Electroneutral compounds
    • C11D1/90Betaines

Definitions

  • the invention relates to basic amino or ammonium antimicrobial agent (especially bisbiguanide, quaternary ammonium salt and bispyridine)-polyethylene glycol ester surfactantbetaine and/or amine oxide surfactants antimicrobial skin cleansing compositions and method of use thereof.
  • Antimicrobial aryl bisbiguanides (U.S. Pat. No. 2,684,924; U.S. Pat. No. 4,053,636) including chlorhexidine (The Merck Index, Ninth Edition, 1976, monograph 2060) and chlorhexidine digluconate salt (U.S. Pat. No. 2,990,425) are known.
  • U.S. Pat. No. 3,855,140 describes polyoxyethylene-polyoxypropylene block copolymer cleansing compositions of certain chlorhexidine salts including chlorhexidine digluconate salt.
  • Antimicrobial alkyl bisbiguanides (U.S. Pat. No.
  • 3,468,898 including alexidine (The Merck Index, ibid., monograph 224) and aqueous compositions thereof with "a compatible surfactant or surfactant mixture selected from the cationic, non-ionic and amphoteric surfactants" (Belgian Pat. No. 862,808) and cycloalkyl bisbiguanides (U.S. Pat. No. 4,022,834) are also known.
  • the quaternary ammonium disinfectants are a well-known class of antimicrobial agents. Particularly well-known examples are benzalkonium chloride (The Merck Index, ibid., monograph 1059), benzethonium chloride (ibid., monograph 1078), cetylpyridinium chloride (ibid., monograph 1987), dequalinium chloride (ibid., monograph 2874) and N-myristyl-N-methylmorpholinium methyl sulfate.
  • benzalkonium chloride The Merck Index, ibid., monograph 1059
  • benzethonium chloride ibid., monograph 1078
  • cetylpyridinium chloride ibid., monograph 1987
  • dequalinium chloride ibid., monograph 2874
  • N-myristyl-N-methylmorpholinium methyl sulfate N-myristyl-N-methylmorpholinium methyl sulfate.
  • Antimicrobial bispyridines and compositions thereof "with any compatible pharmaceutically acceptable surfactant, preferably a non-ionic surfactant, such as the polyoxyethylene polyoxypropylene copolymers . . . amine oxides, such as stearyl dimethyl amine oxide . . . or with mixtures of these" are described by British Pat. No. 1,533,952.
  • Amidinoureas are described by U.S. Pat. No. 4,045,483.
  • U.S. Pat. No. 3,940,441 describes bisphenoxybenzyldiamines.
  • U.S. Pat. No. 3,775,477, U.S. Pat. No. 3,867,454 and U.S. Pat. No. 4,140,860 describe dioldiamines.
  • the polyethylene glycol or polyoxyethylene esters of fatty acids are a known class of surfactants (W. B. Satkowski, S. K. Huang and R. L. Liss in Nonionic Surfactants, Martin J. Schick, Editor, Marcel Dekker, Inc., New York, 1967, pp. 142-174) of the nonionic type, members of which are listed by trade name (McCutcheon's Detergents & Emulsifiers, North American Edition, McCutcheon Division, MC Publishing Co., 175 Rock Road, Glen Rock, N.J. 07452, 1977) and generic name (CTFA Cosmetic Ingredient Dictionary, Second Edition, The Cosmetic, Toiletry and Fragrance Association, Inc., 1133 Fifteenth Street, N.W., Washington, D.C. 20005, 1977).
  • the betaine surfactants are also known and are of the amphoteric type (McCutcheon's Detergents & Emulsifiers, ibid.; CTFA Cosmetic Ingredient Dictionary, ibid.). Members of the class have antimicrobial properties as well as surfactant properties (Seymour S. Block, Disinfection, Sterilization, and Preservation, ibid., pp. 348-360).
  • the amine oxide detergents are also known and are of the nonionic type (AROMOX Amine Oxides, Product Data Bulletin No. 74-21 of Armak Company, Box 1805, Chicago, Ill., 60690, 1974; McCutcheon's Detergents and Emulsifiers, ibid., CTFA Cosmetic Ingredient Dictionary, ibid.).
  • composition of matter is an antimicrobial skin cleansing composition consisting essentially of
  • R 1 is long-chain alkyl or aralkyl
  • R 2 is short-chain alkyl, long-chain alkyl or aralkyl, benzyl or part of an aromatic system or non-aromatic system
  • R 3 and R 4 are short-chain alkyl or part of an aromatic ring system or non-aromatic ring system
  • Z is a carbonhydrogen chain
  • X is a pharmaceutically acceptable anion
  • R is an alkyl group containing from 6 to 18 carbon atoms, a cycloalkyl group containing from 5 to 7 carbon atoms, benzyl, benzyl substituted by one or two substituents selected from the group consisting of halogen, hydroxy, lower-alkyl lower-alkoxy, nitro, cyano and trifluoromethyl or phenyl substituted by methylenedioxy or one or two substituents selected from the group consisting of halogen, lower-alkyl, lower-alkoxy, nitro, cyano and trifluoromethyl;
  • Y is an alkylene group containing from 4 to 18 carbon atoms and separating the two 4-(R,NH)-1-pyridinyl groups by from 4 to 18 carbon atoms;
  • A is a pharmaceutically acceptable anion
  • n 1 or 3;
  • n 1 or 2;
  • x is 1, 2 or 3;
  • amine oxides having the structural formula ##STR6## wherein R 1 taken alone is methyl, ethyl or 2-hydroxyethyl; R 2 taken alone is methyl, ethyl or 2-hydroxyethyl; R 1 and R 2 taken together are morpholino; R 3 is alkyl having from about 8 to about 18 carbon atoms or R 4 CONH(CH 2 ) 3 wherein R 4 is alkyl having from about 8 to about 18 carbon atoms, and wherein 2-hydroxyethyl can be condensed with from 1 to about 200 units of ethylene oxide; and
  • the preferred amount of antimicrobial agent in the composition is from about 0.01% to about 10% by weight of the composition.
  • the invention is an antimicrobial skin cleansing composition consisting essentially of
  • the preferred amount of antimicrobial agent in the composition is from about 0.01% to about 10% by weight of the composition.
  • compositions are generally and, in most instances, particularly described by the prior art cited above.
  • the compositions are prepared by conventional pharmaceutical methods and in addition to the essential ingredients may also include pharmaceutical adjuncts, for example, emollients, lubricants, stabilizers, dyes, perfumes and preservatives. It spite of the presence of the antimicrobial agent, a preservative may be necessary to prevent growth of microorganisms in the compositions.
  • a pharmaceutically acceptable acid or base may also be added for pH adjustment.
  • Particularly preferred bisbiguanides are the following compounds of Formula I.
  • the compound of Formula Ia is chlorhexidine (The Merck Index, ibid., monograph 2060).
  • the compound of Formula Ic is alexidine (ibid., monograph 224).
  • the digluconate salt (chlorhexidine gluconate) is a particularly preferred bisbiguanide salt.
  • a particularly preferred quaternary ammonium salt is benzalkonium chloride (ibid., monograph 1059; R is tetradecyl) (Roccal MC-14 Dihydrate:McCutcheon's Detergents and Emulsifiers, ibid., p. 214), which has the structural formula ##STR8##
  • any polyethylene glycol ester surfactant of Formula V, Formula VI or Formula VII and any betaine surfactant of Formula VIII, Formula IX, Formula X or Formula XI and any amine oxide surfactant of Formula XII can be used in carrying out this invention, specific members of each class may show advantages in specific application areas. Some that have been commercialized only recently are not listed in the current editions of CTFA Cosmetic Ingredient Dictionary (ibid.) or McCutcheon's Detergents & Emulsifiers (ibid.).
  • Particularly preferred polyethylene glycol ester surfactants are tabulated below.
  • PEG-150 Laurate is the compound of Formula V wherein R is undecyl and n has an average value of 150.
  • PEG-150 Distearate is the compound of Formula VI wherein R is heptadecyl and n has an average value of 150.
  • PEG-78 Glyceryl Cocoate is the compound of Formula VII wherein ##STR10## represents the coconut acid radical and n has an average value of 78.
  • PEG-30 Glyceryl Cocoate is the compound of Formula VII wherein ##STR11## represents the coconut acid radical and n has an average value of 30.
  • Particularly preferred betaine surfactants are tabulated below.
  • Coco-betaine is the compound of Formula VIII wherein R 1 represents the coconut radical. Coco-sultaine is the compound of Formula IX wherein R 1 represents the coconut radical. Cocamidopropyl Betaine is the compound of Formula X wherein n is 3, ##STR12## represents the coconut acid radical and R 2 is methyl. Cocamidopropyl Sultaine is the compound of Formula XI wherein is 3, ##STR13## represents the coconut acid radical and R 2 is methyl. .[.Amphoteric-1.]. .Iadd.Cocoamphopropionate .Iaddend.is the compound of Formula XII wherein R 1 represents the coconut radical, R 3 is 2-hydroxyethyl and R 4 is .[.CH 2 COO - ..]. .Iadd.CH 2 CH 2 COONa..Iaddend.
  • Particularly preferred amine oxide surfactants are tabulated below.
  • Myristamine Oxide is the compound of Formula XIII wherein R 1 is methyl, R 2 is methyl and R 3 is tetradecyl. Coco-morpholine Oxide is the compound of Formula XIII wherein R 1 and R 2 taken together are morpholino and R 3 represents the coconut radical.
  • Cocamidopropylamine Oxide is the compound of Formula I wherein R 1 is methyl, R 2 is methyl and R 3 is R 4 CONH(CH 2 ) 3 wherein R 4 CO represents the coconut acid radical.
  • Dihydroxyethyl Cocamine Oxide is the compound of Formula XIII wherein R 1 is 2-hydroxyethyl, R 2 is 2-hydroxyethyl and R 3 is the coconut radical.
  • Example 2 The composition of Example 2 was tested for antimicrobial effect on human hands using a gloved-hand model (R. N. Michaud, M. B. McGrath and W. A. Goss, Journal of Clinical Microbiology, vol. 3, 1976, pp. 406-413). Twelve hands were randomly assigned for washes with the composition of Example 2, and twelve hands were randomly assigned for washes with a corresponding composition differing only by absence of the antimicrobial agent (control). Each hand was washed four times within six hours with a minimum of one hour between washes using five milliliters of each composition for each wash. During each wash the opposite hand of each person was protected with a surgical glove.
  • a gloved-hand model R. N. Michaud, M. B. McGrath and W. A. Goss, Journal of Clinical Microbiology, vol. 3, 1976, pp. 406-413. Twelve hands were randomly assigned for washes with the composition of Example 2, and twelve hands were randomly assigned for washes with a corresponding composition differing only by
  • Microbiological samples for quantitative determination of aerobic resident bacteria were obtained from each hand immediately prior to the first wash (Wash 1 Pre), after a glove-wearing period of one hour subsequent to the first (Wash 1 T 1 ), second (Wash 2 T 1 ) and fourth (Wash 4 T 1 ) washes, and after a non-glove-wearing period of twenty hours after the fourth wash (Wash 4 T 20 ).
  • Total bacterial counts per hand were expressed as logarithms to the base 10.
  • the hand-degerming effect after one wash was evaluated as the bacterial reduction (Wash 1 Pre-Wash 1 T 1 ), abbreviated (W 1 Pre-W 1 T 1 ).
  • Microbiological samples were taken by extracting each gloved hand with sampling fluid (100 ml. of 0.1% Triton X-100 in 0.074 M phosphate buffer at pH 7.8 ⁇ 0.1). An aliquot (10 ml.) of each extract was mixed with chilled neutralizer (10 ml. of 0.0003 M potassium dihydrogen phosphate buffer of pH 7.2 containing 2% Tamol N Micro brand of sodium salt of condensed naphthalene sulfonic acid anionic dispersant).
  • Decimal dilutions (10 -1 , 10 -2 , 10 -3 ) of the neuturalized aliquot were prepared in 0.0003 M KH 2 PO 4 buffer, pH 7.2, and were plated in triplicate with trypticase soy agar using the pour-plate technique. Plates were incubated aerobically at 30° ⁇ 2° C. for 48-72 hours. A total colony count for each plate was determined using an automated colony counter. Average colony counts were used to estimate the total number of bacteria recovered per hand.
  • compositions aspect of the invention are clear as contrasted with opaque.
  • compositions according to the invention can be opacified and emulsified as illustrated by the following examples.
  • Example 6 is the same as Example 6 except tht the percent by weight of chlorhexidine gluconate is increased to 4.00.
  • compositions compared in this test were the composition of Example 1 of the present specification, the composition of Example 1 of the present specification excluding chlorhexidine gluconate, and Hibiclens, which is a commercial polyoxyethylene-polyoxypropylene block copolymer antimicrobial skin cleansing composition containing chlorhexidine gluconate and which is considered to be the composition described by EXAMPLE 1 of above-cited U.S. Pat. No. 3,855,140.
  • the composition of Example 1 of the present specification excluding chlorhexidine gluconate was tested in this test in order to show that the absence of the chlorhexidine gluconate does not significantly affect sudsability.
  • Example 1 of the present specification could not be evaluated in the in vivo sudsing attribute evaluation described below, which was conducted in humans.
  • test composition (0.25 g.) was diluted to 100 ml. with artificially hardened water (0.3 g. calcium chloride in 1 l. of distilled water) in a 250 ml. stoppered graduate cylinder.
  • Lanolin oil (0.05 g.) was added to simulate the natural oil of human hands. The cylinder was rotated end over end at a constant rate and the foam volume was read after 25, 50, 75 and 100 rotations. Five cylinders were used for each composition. The mean foam volumes and standard deviations for each composition were calculated, affording the following results:
  • the means for Example 1 with or without chlorhexidine gluconate are significantly different from the means for Hibiclens (P ⁇ 0.01).
  • the means for Example 1 with chlorhexidine gluconate are not significantly different from the means for Example 1 without chlorhexidine gluconate (P ⁇ 0.1).
  • the five criterion values were assigned the following numerical values: poor, 0; fair, 25; mediocre, 50; good, 75; excellent, 100.
  • the criterion values for each criterion were averaged and gave the following results:

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Abstract

Basic amino or ammonium antimicrobial agent (especially bisbiguanide, quarternary ammonium salt and bispyridine)-polyethylene glycol ester surfactant-betaine and/or amine oxide surfactant antimicrobial skin cleansing compositions and method of use thereof are disclosed.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS
.[.This application.]. .Iadd.This is an application for reissue of U.S. Pat. No. 4,420,484, which issued Dec. 13, 1983 from application Ser. No. 320,754 filed Nov. 12, 1981, which .Iaddend.is a continuation-in-part of our copending application Ser. No. 245,089 filed Mar. 18, 1981 .Iadd.and .Iaddend.now abandoned, which is a continuation-in-part of our copending application Ser. No. 158,737 filed June 12, 1980 and now abandoned, which is a continuation-in-part of our copending application Ser. No. 65,885 filed Aug. 13, 1979 and now abandoned.
BACKGROUND OF THE INVENTION
1. Field of the Invention
The invention relates to basic amino or ammonium antimicrobial agent (especially bisbiguanide, quaternary ammonium salt and bispyridine)-polyethylene glycol ester surfactantbetaine and/or amine oxide surfactants antimicrobial skin cleansing compositions and method of use thereof.
2. Description of the Prior Art
Antimicrobial aryl bisbiguanides (U.S. Pat. No. 2,684,924; U.S. Pat. No. 4,053,636) including chlorhexidine (The Merck Index, Ninth Edition, 1976, monograph 2060) and chlorhexidine digluconate salt (U.S. Pat. No. 2,990,425) are known. U.S. Pat. No. 3,855,140 describes polyoxyethylene-polyoxypropylene block copolymer cleansing compositions of certain chlorhexidine salts including chlorhexidine digluconate salt. Antimicrobial alkyl bisbiguanides (U.S. Pat. No. 3,468,898) including alexidine (The Merck Index, ibid., monograph 224) and aqueous compositions thereof with "a compatible surfactant or surfactant mixture selected from the cationic, non-ionic and amphoteric surfactants" (Belgian Pat. No. 862,808) and cycloalkyl bisbiguanides (U.S. Pat. No. 4,022,834) are also known.
The quaternary ammonium disinfectants (A. N. Petrocci, Disinfection, Sterilization, and Preservation, 2nd Edition, Seymour S. Block, Editor, Lea & Febiger, Philadelphia, 1977, pp. 325-347) are a well-known class of antimicrobial agents. Particularly well-known examples are benzalkonium chloride (The Merck Index, ibid., monograph 1059), benzethonium chloride (ibid., monograph 1078), cetylpyridinium chloride (ibid., monograph 1987), dequalinium chloride (ibid., monograph 2874) and N-myristyl-N-methylmorpholinium methyl sulfate.
Antimicrobial bispyridines and compositions thereof "with any compatible pharmaceutically acceptable surfactant, preferably a non-ionic surfactant, such as the polyoxyethylene polyoxypropylene copolymers . . . amine oxides, such as stearyl dimethyl amine oxide . . . or with mixtures of these" are described by British Pat. No. 1,533,952.
Numerous other basic amino and ammonium antimicrobial agents are described by the prior art, as illustrated by the following examples. Amidinoureas are described by U.S. Pat. No. 4,045,483. U.S. Pat. No. 3,940,441 describes bisphenoxybenzyldiamines. U.S. Pat. No. 3,775,477, U.S. Pat. No. 3,867,454 and U.S. Pat. No. 4,140,860 describe dioldiamines.
The polyethylene glycol or polyoxyethylene esters of fatty acids are a known class of surfactants (W. B. Satkowski, S. K. Huang and R. L. Liss in Nonionic Surfactants, Martin J. Schick, Editor, Marcel Dekker, Inc., New York, 1967, pp. 142-174) of the nonionic type, members of which are listed by trade name (McCutcheon's Detergents & Emulsifiers, North American Edition, McCutcheon Division, MC Publishing Co., 175 Rock Road, Glen Rock, N.J. 07452, 1977) and generic name (CTFA Cosmetic Ingredient Dictionary, Second Edition, The Cosmetic, Toiletry and Fragrance Association, Inc., 1133 Fifteenth Street, N.W., Washington, D.C. 20005, 1977).
The betaine surfactants are also known and are of the amphoteric type (McCutcheon's Detergents & Emulsifiers, ibid.; CTFA Cosmetic Ingredient Dictionary, ibid.). Members of the class have antimicrobial properties as well as surfactant properties (Seymour S. Block, Disinfection, Sterilization, and Preservation, ibid., pp. 348-360).
The amine oxide detergents are also known and are of the nonionic type (AROMOX Amine Oxides, Product Data Bulletin No. 74-21 of Armak Company, Box 1805, Chicago, Ill., 60690, 1974; McCutcheon's Detergents and Emulsifiers, ibid., CTFA Cosmetic Ingredient Dictionary, ibid.).
There is a need for antimicrobial skin cleansing compositions having better sudsing ability than the compositions of the prior art, especially those of above-cited U.S. Pat. No. 3,855,140. The presently described and claimed invention is designed to meet this need.
SUMMARY OF THE INVENTION
In a composition of matter aspect the invention is an antimicrobial skin cleansing composition consisting essentially of
(A) an antimicrobially effective amount of one or more antimicrobial agents selected from the group consisting of
(a) a compound having the structural formula ##STR1## wherein R taken alone is phenyl substituted by alkyl, alkoxy, nitro or halo, p-(2,2-dichlorocyclopropyl)phenyl, alkyl having from 6 to 16 carbon atoms, cycloalkyl or polycyclic alkyl having more than 6 carbon atoms or lower-alkyl-cycloalkyl or cycloalkyl-lower-alkyl having from 1 to 4 carbons in lower alkyl; R' taken alone is hydrogen; R and R' taken together are 3-azabicyclo(3,2,2)nonyl; and n is an integer from 3 to 9; or a pharmaceutically acceptable salt thereof;
(b) a compound having the structural formula ##STR2## wherein R1 is long-chain alkyl or aralkyl; R2 is short-chain alkyl, long-chain alkyl or aralkyl, benzyl or part of an aromatic system or non-aromatic system; R3 and R4 are short-chain alkyl or part of an aromatic ring system or non-aromatic ring system; Z is a carbonhydrogen chain; and X is a pharmaceutically acceptable anion; and
(c) a compound having the structural formula ##STR3## wherein R is an alkyl group containing from 6 to 18 carbon atoms, a cycloalkyl group containing from 5 to 7 carbon atoms, benzyl, benzyl substituted by one or two substituents selected from the group consisting of halogen, hydroxy, lower-alkyl lower-alkoxy, nitro, cyano and trifluoromethyl or phenyl substituted by methylenedioxy or one or two substituents selected from the group consisting of halogen, lower-alkyl, lower-alkoxy, nitro, cyano and trifluoromethyl;
Y is an alkylene group containing from 4 to 18 carbon atoms and separating the two 4-(R,NH)-1-pyridinyl groups by from 4 to 18 carbon atoms;
A is a pharmaceutically acceptable anion;
m is 1 or 3;
n is 1 or 2;
x is 1, 2 or 3; and
(m)(2)=(n)(x);
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants having the structural formulas ##STR4## wherein R is alkyl or alkenyl having from about 8 to about 20 carbon atoms or lanolin and n is an integer from about 8 to about 200;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from
(a) betaines having the structural formulas ##STR5## wherein R1 is alkyl or alkenyl having from about 8 to about 18 carbon atoms; R2 is methyl, ethyl or 2-hydroxyethyl; R3 is 2-hydroxyethyl .[.or CH2 COO- .].; R4 is .[.CH2 COO- or CH2 CH2 --O--CH2 COO- ;.]. .Iadd.CH2 CH2 COONa; .Iaddend.and n is 2 or 3; and
(b) amine oxides having the structural formula ##STR6## wherein R1 taken alone is methyl, ethyl or 2-hydroxyethyl; R2 taken alone is methyl, ethyl or 2-hydroxyethyl; R1 and R2 taken together are morpholino; R3 is alkyl having from about 8 to about 18 carbon atoms or R4 CONH(CH2)3 wherein R4 is alkyl having from about 8 to about 18 carbon atoms, and wherein 2-hydroxyethyl can be condensed with from 1 to about 200 units of ethylene oxide; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
The preferred amount of antimicrobial agent in the composition is from about 0.01% to about 10% by weight of the composition.
In a process aspect the invention is the process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition consisting essentially of
(A) from about 0.01% to about 10% by weight of one or more antimicrobial agents selected from the group consisting of
(a) a compound of Formula I,
(b) a compound of Formula II or Formula III,
(c) a compound of Formula IV;
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants of Formula V, Formula VI and Formula VII;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from
(a) betaines of Formula VIII, Formula IX, Formula X, Formula XI and Formula XII; and
(b) amine oxides of Formula XIII; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
In a broader composition of matter aspect the invention is an antimicrobial skin cleansing composition consisting essentially of
(A) an antimicrobially effective amount of a basic amino or ammonium antimicrobial agent;
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants of Formula V, Formula VI and Formula VIII;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from
(a) betaines of Formula VIII, Formula IX, Formula X, Formula XI and Formula XII; and
(b) amine oxides of formula XIII; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
The preferred amount of antimicrobial agent in the composition is from about 0.01% to about 10% by weight of the composition.
In a broader process aspect the invention is the method of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition consisting essentially of
(A) from about 0.01% to about 10% by weight of a basic amino or ammonium antimicrobial agent;
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants of Formula V, Formula VI and Formula VII;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from
(a) betaines of Formula VIII, Formula IX, Formula X, Formula XI and Formula XII; and
(b) amine oxides of Formula XIII; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
DETAILED DESCRIPTION OF THE INVENTION INCLUSIVE OF THE PREFERRED EMBODIMENTS Compositions
The essential ingredients of the compositions are generally and, in most instances, particularly described by the prior art cited above. The compositions are prepared by conventional pharmaceutical methods and in addition to the essential ingredients may also include pharmaceutical adjuncts, for example, emollients, lubricants, stabilizers, dyes, perfumes and preservatives. It spite of the presence of the antimicrobial agent, a preservative may be necessary to prevent growth of microorganisms in the compositions. A pharmaceutically acceptable acid or base may also be added for pH adjustment.
Particularly preferred bisbiguanides are the following compounds of Formula I.
______________________________________                                    
Compound of                                                               
Formula    R                   R'    n                                    
______________________________________                                    
Ia         p-ClC.sub.6 H.sub.5 H     6                                    
Ib                                                                        
            ##STR7##           H     6                                    
Ic         CH.sub.3 (CH.sub.2).sub.3 CH(CH.sub.2 CH.sub.3)CH.sub.2        
                               H     6                                    
Id         CH.sub.3 (CH.sub.2).sub.6                                      
                               H     6                                    
______________________________________                                    
The compound of Formula Ia is chlorhexidine (The Merck Index, ibid., monograph 2060). The compound of Formula Ic is alexidine (ibid., monograph 224). The digluconate salt (chlorhexidine gluconate) is a particularly preferred bisbiguanide salt.
A particularly preferred quaternary ammonium salt is benzalkonium chloride (ibid., monograph 1059; R is tetradecyl) (Roccal MC-14 Dihydrate:McCutcheon's Detergents and Emulsifiers, ibid., p. 214), which has the structural formula ##STR8##
The following bispyridines of Formula IV are particularly preferred.
______________________________________                                    
Com-                                                                      
pound of                                                                  
Formula                                                                   
       R             Y        A      m   n   %                            
______________________________________                                    
IVa    CH.sub.3 (CH.sub.2).sub.6                                          
                     (CH.sub.2).sub.12                                    
                              Cl or Br                                    
                                     1   2   1                            
IVb    CH.sub.3 (CH.sub.2).sub.7                                          
                     (CH.sub.2).sub.10                                    
                              Cl or Br                                    
                                     1   2   1                            
IVc                                                                       
        ##STR9##     (CH.sub.2).sub.12                                    
                              Cl or Br                                    
                                     1   2   1                            
______________________________________                                    
Although any polyethylene glycol ester surfactant of Formula V, Formula VI or Formula VII and any betaine surfactant of Formula VIII, Formula IX, Formula X or Formula XI and any amine oxide surfactant of Formula XII can be used in carrying out this invention, specific members of each class may show advantages in specific application areas. Some that have been commercialized only recently are not listed in the current editions of CTFA Cosmetic Ingredient Dictionary (ibid.) or McCutcheon's Detergents & Emulsifiers (ibid.).
Particularly preferred polyethylene glycol ester surfactants are tabulated below.
______________________________________                                    
                 CTFA Cosmetic Ingredient                                 
Generic Name     Dictionary Page                                          
______________________________________                                    
PEG-150 Laurate  219                                                      
PEG-150 Distearate                                                        
                 211                                                      
PEG-78 Glyceryl Cocoate                                                   
                 212                                                      
(Cf. PEG-7 Glyceryl                                                       
Cocoate)                                                                  
PEG-30 Glyceryl Cocoate                                                   
                 212                                                      
(Cf. PEG-7 Glyceryl                                                       
Cocoate)                                                                  
______________________________________                                    
PEG-150 Laurate is the compound of Formula V wherein R is undecyl and n has an average value of 150. PEG-150 Distearate is the compound of Formula VI wherein R is heptadecyl and n has an average value of 150. PEG-78 Glyceryl Cocoate is the compound of Formula VII wherein ##STR10## represents the coconut acid radical and n has an average value of 78. PEG-30 Glyceryl Cocoate is the compound of Formula VII wherein ##STR11## represents the coconut acid radical and n has an average value of 30.
Particularly preferred betaine surfactants are tabulated below.
______________________________________                                    
                          CTFA                                            
                          Cosmetic Ingredient                             
Generic Name  Type        Dictionary Page                                 
______________________________________                                    
Coco-betaine  Formula VIII                                                
                          67                                              
Coco-sultaine Formula IX  68                                              
Cocamidopropyl                                                            
              Formula X   65                                              
Betaine                                                                   
Cocamidopropyl                                                            
              Formula XI  66                                              
Sultaine                                                                  
.[.Amphoteric-l                                                           
              Formula XII 24.].                                           
.[.Cocoamphoglycinate                                                     
              Formula XII 55*.].                                          
.Iadd.Cocoamphopropionate                                                 
              Formula XII  55*                                            
______________________________________                                    
 *CTFA Cosmetic Ingredient Dictionary, Third Edition, The Cosmetic,       
 Toiletry and Fragrance Association, Inc., 1110 Vermont Avenue, N.W.,     
 Washington, D.C. 20005, 1982  .Iaddend.                                  
Coco-betaine is the compound of Formula VIII wherein R1 represents the coconut radical. Coco-sultaine is the compound of Formula IX wherein R1 represents the coconut radical. Cocamidopropyl Betaine is the compound of Formula X wherein n is 3, ##STR12## represents the coconut acid radical and R2 is methyl. Cocamidopropyl Sultaine is the compound of Formula XI wherein is 3, ##STR13## represents the coconut acid radical and R2 is methyl. .[.Amphoteric-1.]. .Iadd.Cocoamphopropionate .Iaddend.is the compound of Formula XII wherein R1 represents the coconut radical, R3 is 2-hydroxyethyl and R4 is .[.CH2 COO-..]. .Iadd.CH2 CH2 COONa..Iaddend.
Particularly preferred amine oxide surfactants are tabulated below.
______________________________________                                    
                  CTFA Cosmetic Ingredient                                
Generic Name      Dictionary Page                                         
______________________________________                                    
Myristamine Oxide 179                                                     
Coco-morpholine Oxide                                                     
                  67                                                      
Cocamidopropylamine Oxide                                                 
                  65                                                      
Dihydroxyethyl Cocamine Oxide                                             
                  95                                                      
______________________________________                                    
Myristamine Oxide is the compound of Formula XIII wherein R1 is methyl, R2 is methyl and R3 is tetradecyl. Coco-morpholine Oxide is the compound of Formula XIII wherein R1 and R2 taken together are morpholino and R3 represents the coconut radical. Cocamidopropylamine Oxide is the compound of Formula I wherein R1 is methyl, R2 is methyl and R3 is R4 CONH(CH2)3 wherein R4 CO represents the coconut acid radical. Dihydroxyethyl Cocamine Oxide is the compound of Formula XIII wherein R1 is 2-hydroxyethyl, R2 is 2-hydroxyethyl and R3 is the coconut radical.
Compatibility
Compatability of the antimicrobial agents of Formula Id, Formula IIa, Formula IVa (A=Br), Formula IVb (A=Br) and Formula IVc (A=Br) with a prototype vehicle of the invention was tested by a serial dilution test against Staphylococcus aureus ATCC 6538. Volumes of two milliliters were used. Dilutions were in tryptosephosphate broth. Incubation was for 16-18 hours at 37° C. Growth was verified with 2,3,5-triphenyltetrazolium chloride. The inoculum was 1.8×105 viable cells per tube. There follows the formula of the prototype vehicle.
______________________________________                                    
Ingredient        Percent by Weight                                       
______________________________________                                    
PEG-150 Laurate   12.0                                                    
Isopropyl Alcohol*                                                        
                  3.22                                                    
Cocamidopropyl Betaine                                                    
                  3.00                                                    
Laneth-16         1.00                                                    
Edetate Disodium  0.500                                                   
PEG-150 Distearate                                                        
                  0.500                                                   
PEG-2M            0.100                                                   
Perfume           0.100                                                   
FD&C Blue No. 1     0.000800                                              
FD&C Yellow No. 5   0.000320                                              
Gluconic Acid or Sodium                                                   
                  pH about 5.5                                            
Hydroxide to make                                                         
Purified Water to make                                                    
                  100.0                                                   
______________________________________                                    
 Laneth-16 is the polyethylene glycol ether of Lanolin Alcohol with an    
 average ethoxylation value of 16. PEG2M is the polymer of ethylene oxide 
 having the structural formula H(OCH.sub.2 CH.sub.2).sub.n OH wherein n ha
 an average value of 2000.                                                
 *To make 4% by volume                                                    
The following results were obtained.
______________________________________                                    
Added  Minimum Inhibitory Concentration (MIC) (μg/ml)                  
Vehicle                                                                   
       of Compound of Formula                                             
(μg/ml)                                                                
       Id.sup.+ IIa      IVa°                                      
                                IVb°                               
                                        IVc°                       
______________________________________                                    
  0    0.25     0.5      0.39   0.5     0.25                              
 15.6  0.25     0.5      0.195  0.5     0.25                              
 31.2  0.25     0.5      0.39   0.5     0.25                              
 62.5  0.25     0.5      0.39   0.5     0.25                              
 125.0 0.25     0.5      0.10   0.5     0.125                             
 250.0 0.25     0.5      0.10   0.25    0.0625                            
 500.0 0.25     0.5      0.10   0.125   0.125                             
1000.0 0.25     1.0      0.195  0.125   0.125                             
2000.0 0.50     1.0      0.10   0.125   0.125                             
4000.0 0.50      1.95    0.195  0.25    0.25                              
8000.0 0.50      1.95    0.10   0.25    0.25                              
16000.0                                                                   
       *        *        *      *       *                                 
32000.0                                                                   
       **       **       **     **      **                                
64000.0                                                                   
       **       **       **     **      **                                
______________________________________                                    
 .sup.+ Expressed as free base                                            
 °Expressed as cation                                              
 *MIC of vehicle alone                                                    
 **No growth at this concentration of vehicle                             
It was concluded that each of the five antimicrobial agents tested was compatible with the prototype vehicle.
EXAMPLES
The following examples still more particularly describe the compositions of the invention.
EXAMPLE 1
______________________________________                                    
Ingredient        Percent by Weight                                       
______________________________________                                    
Chlorhexidine Gluconate                                                   
                  4.00                                                    
PEG-78 Glyceryl Cocoate                                                   
                  10.0                                                    
.[.Amphoteric-l   5.00.].                                                 
.Iadd.Cocoamphopropionate                                                 
                  5.00.Iaddend.                                           
Laneth-16         1.00                                                    
Benzyl Alcohol    1.00                                                    
Acetamide MEA     0.750                                                   
PEG-30 Glyceryl Cocoate                                                   
                  0.500                                                   
Color             0.00100                                                 
Perfume           0.0500                                                  
Gluconic Acid to make pH                                                  
                  2.19                                                    
about 5.5, about                                                          
Purified Water to make                                                    
                  100.0                                                   
______________________________________                                    
 Acetamide MEA is N--(2hydroxyethyl)acetamide.                            
EXAMPLE 2
______________________________________                                    
Ingredient          Percent by Weight                                     
______________________________________                                    
Compound of Formula IVb (A = Cl)                                          
                    2.00                                                  
PEG-150 Laurate     12.0                                                  
Isopropyl Alcohol   3.22                                                  
Cocamidopropyl Betaine                                                    
                    5.00                                                  
Laneth-16           1.00                                                  
Edetate Disodium    0.500                                                 
PEG-150 Distearate  0.500                                                 
PEG-2M              0.100                                                 
Perfume             0.100                                                 
Color               0.00100                                               
Sodium Hydroxide to make                                                  
                    0.023                                                 
pH about 5.5                                                              
Purified Water to make                                                    
                    100.00                                                
______________________________________                                    
EXAMPLE 3
______________________________________                                    
Ingredient           Percent by Weight                                    
______________________________________                                    
Compound of Formula IVb                                                   
                     2.00                                                 
(A = Cl)                                                                  
PEG-78 Glyceryl Cocoate                                                   
                     10.0                                                 
Dihydroxyethyl Cocoamine Oxide                                            
                     5.00                                                 
Citric Acid          0.384                                                
Sodium Hydroxide to make                                                  
                     --                                                   
pH about 5.5                                                              
Purified Water to make                                                    
                     100.00                                               
______________________________________                                    
EXAMPLE 4
______________________________________                                    
Ingredient         Percent by Weight                                      
______________________________________                                    
Chlorhexidine Gluconate                                                   
                   4.00                                                   
.[.Amphoteric-l    2.50.].                                                
.Iadd.Cocoamphopropionate                                                 
                   2.50.Iaddend.                                          
Cocamidopropylamine Oxide                                                 
                   2.00                                                   
PEG-78 Glyceryl Cocoate                                                   
                   10.0                                                   
PEG-30 Glyceryl Cocoate                                                   
                   0.500                                                  
Laneth-16          1.00                                                   
Acetamide MEA      0.750                                                  
Color              0.00100                                                
Perfume            0.0500                                                 
Gluconic Acid or Sodium Hy-                                               
                   --                                                     
droxide to make pH about 5.5                                              
Acetic Acid        0.500                                                  
Benzyl Alcohol     1.00                                                   
Purified Water to make                                                    
                   100.00                                                 
______________________________________                                    
EXAMPLE 5
______________________________________                                    
Ingredient          Percent by Weight                                     
______________________________________                                    
Chlorhexidine Gluconate                                                   
                    4.00                                                  
Dihydroxyethyl Cocamine Oxide                                             
                    3.00                                                  
PEG-150 Distearate  0.750                                                 
Laneth-16           1.00                                                  
Benzyl Alcohol      2.00                                                  
Perfume             0.100                                                 
Color               0.00100                                               
Gluconic Acid or Sodium Hydroxide                                         
                    --                                                    
to make pH about 5.5                                                      
Purified Water to make                                                    
                    100.00                                                
______________________________________                                    
Method of Use
The composition of Example 2 was tested for antimicrobial effect on human hands using a gloved-hand model (R. N. Michaud, M. B. McGrath and W. A. Goss, Journal of Clinical Microbiology, vol. 3, 1976, pp. 406-413). Twelve hands were randomly assigned for washes with the composition of Example 2, and twelve hands were randomly assigned for washes with a corresponding composition differing only by absence of the antimicrobial agent (control). Each hand was washed four times within six hours with a minimum of one hour between washes using five milliliters of each composition for each wash. During each wash the opposite hand of each person was protected with a surgical glove.
Microbiological samples for quantitative determination of aerobic resident bacteria were obtained from each hand immediately prior to the first wash (Wash 1 Pre), after a glove-wearing period of one hour subsequent to the first (Wash 1 T1), second (Wash 2 T1) and fourth (Wash 4 T1) washes, and after a non-glove-wearing period of twenty hours after the fourth wash (Wash 4 T20). Total bacterial counts per hand were expressed as logarithms to the base 10. The hand-degerming effect after one wash was evaluated as the bacterial reduction (Wash 1 Pre-Wash 1 T1), abbreviated (W1 Pre-W1 T1). Cumulative effects after two washes and four washes were evaluated as the bacterial reductions (Wash 1 Pre-Wash 2 T1), abbreviated (W1 Pre-W2 T1), and (Wash1 Pre-Wash 4 T1), abbreviated (W1 Pre-W4 T1). Persistent effect was evaluated as the bacterial reduction (Wash 1 Pre-Wash 4 T20), abbreviated (W.sub. 1 Pre-W4 T20).
Microbiological samples were taken by extracting each gloved hand with sampling fluid (100 ml. of 0.1% Triton X-100 in 0.074 M phosphate buffer at pH 7.8±0.1). An aliquot (10 ml.) of each extract was mixed with chilled neutralizer (10 ml. of 0.0003 M potassium dihydrogen phosphate buffer of pH 7.2 containing 2% Tamol N Micro brand of sodium salt of condensed naphthalene sulfonic acid anionic dispersant). Decimal dilutions (10-1, 10-2, 10-3) of the neuturalized aliquot were prepared in 0.0003 M KH2 PO4 buffer, pH 7.2, and were plated in triplicate with trypticase soy agar using the pour-plate technique. Plates were incubated aerobically at 30°±2° C. for 48-72 hours. A total colony count for each plate was determined using an automated colony counter. Average colony counts were used to estimate the total number of bacteria recovered per hand.
The following results were obtained
______________________________________                                    
                          Number of Bacteria                              
           Mean (n = 12) Log.sub.10                                       
                          Recovered Per Hand                              
Sample     Control        Example 2                                       
______________________________________                                    
Wash 1 Pre 6.372          6.165                                           
Wash 1 T.sub.1                                                            
           6.537          5.770                                           
Wash 2 T.sub.1                                                            
           6.316          5.030                                           
Wash 4 T.sub.1                                                            
           5.978          4.296                                           
Wash 4 T.sub.20                                                           
           5.846          4.867                                           
______________________________________                                    
                          Bacterial Reductions                            
           Mean (n = 12) Log.sub.10                                       
                          Per Hand                                        
Difference Control        Example 2                                       
______________________________________                                    
W.sub.1 Pre - W.sub.1 T.sub.1                                             
           -0.166         0.394                                           
W.sub.1 Pre - W.sub.2 T.sub.1                                             
           0.055          1.135                                           
W.sub.1 Pre - W.sub.4 T.sub.1                                             
           0.393          1.869                                           
W.sub.1 Pre - W.sub.4 T.sub.20                                            
           0.525          1.297                                           
______________________________________                                    
The foregoing mean log10 bacterial reductions were significantly greater (P≦0.01) for the hands treated with the composition of Example 2 than for the hands treated with the control composition.
Opacified Compositions
The foregoing examples of the compositions aspect of the invention are clear as contrasted with opaque. By addition of appropriate ingredients compositions according to the invention can be opacified and emulsified as illustrated by the following examples.
EXAMPLE 6
______________________________________                                    
Ingredient          Percent by Weight                                     
______________________________________                                    
Chlorhexidine Gluconate                                                   
                    1.00                                                  
PEG-78 Glyceryl Cocoate                                                   
                    10.0                                                  
EMPIGEN OB (Tertiary Alkylamine                                           
                    10.0                                                  
Oxide 30%)                                                                
White Soft Paraffin 6.0                                                   
Dihydroxyethyl Cocamine Oxide                                             
                    5.26                                                  
Acetamide MEA (75%) 1.0                                                   
Benzyl Alcohol      1.0                                                   
Polysorbate 60      1.0                                                   
Sorbitan Stearate   1.00                                                  
Gluconic Acid to make pH 5.8                                              
                    --                                                    
Purified Water to make                                                    
                    100.0                                                 
______________________________________                                    
 Polysorbate 60 is a mixture of stearate esters of sorbitol and sorbitol  
 anhydrides, consisting predominantly of the monoester, condensed with    
 approximately 20 moles of ethylene oxide. Sorbitan Stearate is the       
 monoester of stearic and hexitol anhydrides derived from sorbitol.       
EXAMPLE 7
is the same as Example 6 except tht the percent by weight of chlorhexidine gluconate is increased to 4.00.
Comparative Sudsing Test and Sudsing Attribute Evaluation
In order to show that the presently described and claimed invention achieves its above-stated objective to provide antimicrobial skin cleansing compositions having better sudsing ability than the compositions of the prior art, the following comparative in vitro sudsing test and in vivo sudsing attribute evaluation were conducted.
In Vitro Sudsing Test
The compositions compared in this test were the composition of Example 1 of the present specification, the composition of Example 1 of the present specification excluding chlorhexidine gluconate, and Hibiclens, which is a commercial polyoxyethylene-polyoxypropylene block copolymer antimicrobial skin cleansing composition containing chlorhexidine gluconate and which is considered to be the composition described by EXAMPLE 1 of above-cited U.S. Pat. No. 3,855,140. The composition of Example 1 of the present specification excluding chlorhexidine gluconate was tested in this test in order to show that the absence of the chlorhexidine gluconate does not significantly affect sudsability. This was necessary because, without approval by the United States Food and Drug Administration and due to the presence of the chlorhexidine gluconate, the composition of Example 1 of the present specification could not be evaluated in the in vivo sudsing attribute evaluation described below, which was conducted in humans.
The test composition (0.25 g.) was diluted to 100 ml. with artificially hardened water (0.3 g. calcium chloride in 1 l. of distilled water) in a 250 ml. stoppered graduate cylinder. Lanolin oil (0.05 g.) was added to simulate the natural oil of human hands. The cylinder was rotated end over end at a constant rate and the foam volume was read after 25, 50, 75 and 100 rotations. Five cylinders were used for each composition. The mean foam volumes and standard deviations for each composition were calculated, affording the following results:
______________________________________                                    
Number of                                                                 
         Foam Volume (ml.)                                                
Rotations                                                                 
         25        50        75      100                                  
______________________________________                                    
Example 1                                                                 
         132 ± 4.5                                                     
                   142 ± 7.7                                           
                             164 ± 10.5                                
                                     173 ± 6.6                         
Example 1                                                                 
         127 ± 6.0                                                     
                   135 ± 10.9                                          
                             149 ± 13.7                                
                                     162 ± 22.3                        
without                                                                   
Chlorhexidine                                                             
Gluconate                                                                 
Hibiclens                                                                 
         107 ± 2.3                                                     
                   111 ± 2.6                                           
                             115 ± 2.6                                 
                                     120 ± 3.8                         
______________________________________                                    
The means for Example 1 with or without chlorhexidine gluconate are significantly different from the means for Hibiclens (P<0.01). The means for Example 1 with chlorhexidine gluconate are not significantly different from the means for Example 1 without chlorhexidine gluconate (P<0.1).
In Vivo Sudsing Attribute Evaluation
Since Hibiclens is red in color and the preferred color of the composition of Example 1 of the present specification is green, both a red formulation and a green formulation according to Example 1 (Both lacking chlorhexidine gluconate) were tested. The results were not significantly affected by the difference in color.
Ten persons participated in the evaluation. Each person was instructed to: (1) wash his or her hands with soap and water, (2) rinse the hands and leave them wet, (3) apply one teaspoonful of the skin cleansing compositions to the hands, (4) wash the hands with the skin cleanser for one full minute, adding water as desired, (5) rinse the hands, (6) dry the hands with a towel and (7) repeat steps (1) through (6) three times allowing at least one hour between repetitions.
Each person was instructed to evaluate the skin cleansing composition with regard to four criteria relating to sudsing and to assign one of five values to each criterion as follows: Criteria: initial or flash foam, foam quantity, foam texture, foam stability. Criterion values: poor, fair, mediocre, good, excellent.
The five criterion values were assigned the following numerical values: poor, 0; fair, 25; mediocre, 50; good, 75; excellent, 100. The criterion values for each criterion were averaged and gave the following results:
______________________________________                                    
        Criterion Value                                                   
          Example 1    Example 1                                          
          Without      Without                                            
          Chlorhexidine                                                   
                       Chlorhexidine                                      
          Gluconate    Gluconate  Hibiclens                               
Criterion (Green)      (Red)      (Red)                                   
______________________________________                                    
Initial or                                                                
          78 ± 8    78 ± 8  23 ± 28                              
Flash Foam                                                                
Foam Quantity                                                             
          85 ± 13   78 ± 8  35 ± 32                              
Foam Texture                                                              
          83 ± 17    80 ± 11                                        
                                  35 ± 32                              
Foam Stability                                                            
          83 ± 17   78 ± 8  38 ± 34                              
______________________________________                                    
All of the criterion values for Example 1 without chlorhexidine gluconate are significantly different from those of Hibiclens (P<0.01).

Claims (31)

We claim:
1. An antimicrobial skin cleansing composition consisting essentially of
an antimicrobially effective amount of one or more antimicrobial agents selected from the group consisting of
(a) a compound having the structural formula ##STR14## wherein R taken alone is phenyl substituted by alkyl, alkoxy, nitro or halo, p-(2,2-dichlorocyclopropyl)phenyl, alkyl having from 6 to 16 carbon atoms, cycloalkyl or polycyclic alkyl having more than 6 carbon atoms or lower-alkyl-cycloalkyl or cycloalkyl-lower-alkyl having from 1 to 4 carbons in lower alkyl; R' taken alone is hydrogen; R and R' taken together are 3-azabicyclo(3,2,2)nonyl; and n is an integer from 3 to 9; or a pharmaceutically acceptable salt thereof;
(b) a compound having the structural formula ##STR15## wherein R1 is long-chain alkyl or aralkyl; R2 is short-chain alkyl, long-chain alkyl or aralkyl, benzyl or part of an aromatic system or non-aromatic system; R3 and R4 are short-chain alkyl or part of an aromatic ring system or non-aromatic ring system; Z is a carbon-hydrogen chain; and X is a pharmaceutically acceptable anion; and
(c) a compound having the structural formula ##STR16## wherein R is an alkyl group containing from 6 to 18 carbon atoms, a cycloalkyl group containing from 5 to 7 carbon atoms, benzyl, benzyl substituted by one or two substituents selected from the group consisting of halogen, hydroxy, lower-alkyl, lower-alkoxy, nitro, cyano and trifluoromethyl or phenyl substituted by methylenedioxy or one or two substituents selected from the group consisting of halogen, lower-alkyl, lower-alkoxy, nitro, cyano and trifluoromethyl;
Y is an alkylene group containing from 4 to 18 carbon atoms and separating the two 4-(R-NH)-1-pyridinyl groups by from 4 to 18 carbon atoms;
A is a pharmaceutically acceptable anion;
m is 1 or 3;
n is 1 or 2;
x is 1, 2 or 3; and
(m)(2)=(n)(x);
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants having the structural formulas ##STR17## wherein R is alkyl or alkenyl having from about 8 to about 20 carbon atoms or lanolin and n is an integer from about 8 to about 200;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from the group consisting of
(a) betaines having the structural formulas ##STR18## wherein R1 is alkyl or alkenyl having from about 8 to about 18 carbon atoms; R2 is methyl, ethyl or 2-hydroxyethyl; R3 is 2-hydroxyethyl .[.or CH2 COO- .].; R4 is .[.CH2 COO- or CH2 CH2 --O--CH2 COO- ; an.]. .Iadd.CH2 CH2 COONa; and .Iaddend.n is 2 or 3; and
(b) amine oxides having the structural formula ##STR19## wherein R1 taken alone is methyl, ethyl or 2-hydroxyethyl; R2 taken alone is methyl, ethyl or 2-hydroxyethyl; R1 and R2 taken together are morpholino; R3 is alkyl having from about 8 to about 18 carbon atoms or R4 CONH(CH2)3 wherein R4 is alkyl having from about 8 to about 18 carbon atoms; and wherein 2-hydroxyethyl can be condensed with from 1 to about 200 units of ethylene oxide; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
2. A composition according to claim 1 wherein the amount of antimicrobial agent is from about 0.01% to about 10% by weight of the composition.
3. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 2.
4. An antimicrobial skin cleansing composition consisting essentially of
(A) an antimicrobially effective amount of one or more antimicrobial agents selected from the group consisting of a compound having the structural formula ##STR20## wherein R taken alone is phenyl substituted by alkyl, alkoxy, nitro or halo, p-(2,2-dichlorocyclopropyl)phenyl, alkyl having from 6 to 16 carbon atoms, cycloalkyl or polycyclic alkyl having more than 6 carbon atoms or lower-alkyl-cycloalkyl or cycloalkyl-lower-alkyl having from 1 to 4 carbons in lower alkyl; R' taken alone is hydrogen; R and R' taken together are 3-azabicyclo(3,2,2)nonyl; and n is an integer from 3 to 9; or a pharmaceutically acceptable salt thereof;
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants having the structural formulas ##STR21## wherein R is alkyl or alkenyl having from about 8 to about 20 carbon atoms or lanolin and n is an integer from about 8 to about 200;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from the group consisting of
(a) betaines having the structural formulas ##STR22## wherein R1 is alkyl or alkenyl having from about 8 to about 18 carbon atoms; R2 is methyl, ethyl or 2-hydroxyethyl; R3 is 2-hydroxyethyl .[.or CH2 COO- .].; R4 is .[.CH2 COO- or CH2 CH2 --O--CH2 COO- ;.]. .Iadd.CH2 CH2 COONa; .Iaddend.and n is 2 or 3; and
(b) amine oxides having the structural formula ##STR23## wherein R1 taken alone is methyl, ethyl or 2-hydroxyethyl; R2 taken alone is methyl, ethyl or 2-hydroxyethyl; R1 and R2 taken together are morpholino; R3 is alkyl having from about 8 to about 18 carbon atoms or R4 CONH(CH2)3 wherein R4 is alkyl having from about 8 to about 18 carbon atoms, and wherein 2-hydroxyethyl can be condensed with from 1 to about 200 units of ethylene oxide; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
5. A composition according to claim 4 wherein the amount of antimicrobial agent is from about 0.01% to about 10% by weight of the composition.
6. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 5.
7. A composition according to claim 5 wherein the antimicrobial agent is chlorhexidine or a pharmaceutically acceptable salt thereof.
8. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 7.
9. A composition according to claim 7 wherein the salt is the digluconate salt.
10. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 9.
11. A composition according to claim 9 wherein the surfactant of part (C) is a betaine.
12. A composition according to claim 11 wherein the betaine has the structural formula ##STR24## wherein R1 represents the coconut radical, R3 is 2-hydroxyethyl and R4 is .[.CH2 COO-..]. .Iadd.CH2 CH2 COONa..Iaddend.
13. A composition according to claim 12 which contains two polyethylene glycol ester surfactants of part (B), both having the structural formula ##STR25## wherein ##STR26## represents the coconut acid radical, one wherein n has an average value of 78 and the other wherein n has an average value of 30.
14. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 11.
15. An antimicrobial skin cleansing composition consisting essentially of
(A) an antimicrobially effective amount of one or more antimicrobial agents selected from the group consisting of a compound having the structural formula ##STR27## wherein R1 is long-chain alkyl or aralkyl; R2 is short-chain alkyl, long-chain alkyl or aralkyl, benzyl or part of an aromatic system or non-aromatic system; R3 and R4 are short-chain alkyl or part of an aromatic ring system or non-aromatic ring system; Z is a carbon-hydrogen chain; and X is a pharmaceutically acceptable anion;
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants having the structural formulas ##STR28## wherein R is alkyl or alkenyl having from about 8 to about 20 carbon atoms or lanolin and n is an integer from about 8 to about 200;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from the group consisting of
(a) betaines having the structural formulas ##STR29## wherein R1 is alkyl or alkenyl having from about 8 to about 18 carbon atoms; R2 is methyl, ethyl or 2-hydroxyethyl; R3 is 3-hydroxyethyl .[.or CH2 COO- .].; R4 is .[.CH2 COO- or CH2 CH2 --O--CH2 COO- ;.]. .Iadd.CH2 CH2 COONa; .Iaddend.and n is 2 or 3; and
(b) amine oxides having the structural formula ##STR30## wherein R1 taken alone is methyl, ethyl or 2-hydroxyethyl; R2 taken alone is methyl, ethyl or 2-hydroxyethyl; R1 and R2 taken together are morpholino; R3 is alkyl having from about 8 to about 18 carbon atoms or R4 CONH(CH2)3 wherein R4 is alkyl having from about 8 to about 18 carbon atoms, and wherein 2-hydroxyethyl can be condensed with from 1 to about 200 units of ethylene oxide; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
16. A composition according to claim 15 wherein the amount of antimicrobial agent is from about 0.01% to about 10% by weight of the composition.
17. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 16.
18. A composition according to claim 16 wherein the antimicrobial agent is benzalkonium chloride.
19. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 18.
20. An antimicrobial skin cleansing composition consisting essentially of
(A) an antimicrobially effective amount of one or more antimicrobial agents selected from the group consisting of a compound having the structural formula ##STR31## wherein R is an alkyl group containing from 6 to 18 carbon atoms, a cycloalkyl group containing from 5 to 7 carbon atoms, benzyl, benzyl substituted by one or two substituents selected from the group consisting of halogen, hydroxy, lower-alkyl, lower-alkoxy, nitro, cyano and trifluoromethyl or phenyl substituted by methylenedioxy or one or two substituents selected from the group consisting of halogen, lower-alkyl, lower-alkoxy, nitro, cyano and trifluoromethyl;
Y is an alkylene group containing from 4 to 18 carbon atoms and separating the two 4-(R--NH)-1-pyridinyl groups by from 4 to 18 carbon atoms;
A is a pharmaceutically acceptable anion;
m is 1 or 3;
n is 1 or 2;
x is 1, 2 or 3; and
(m)(2)=(n)(x);
(B) from about 0.75% to about 30% by weight of one or more polyethylene glycol ester surfactants having the structural formulas ##STR32## wherein R is alkyl or alkenyl having from about 8 to about 20 carbon atoms or lanolin and n is an integer from about 8 to about 200;
(C) from about 0.5% to about 30% by weight of one or more surfactants selected from the group consisting of
(a) betaines having the structural formulas ##STR33## wherein R1 is alkyl or alkenyl having from about 8 to about 18 carbon atoms; R2 is methyl, ethyl or 2-hydroxyethyl; R3 is 2-hydroxyethyl .[.or CH2 COO- .].; R4 is .[.CH2 COO- or CH2 CH2 --O--CH2 COO- ; and.]. .Iadd.CH2 CH2 COONa; and .Iaddend.n is 2 or 3; and
(b) amine oxides having the structural formula ##STR34## wherein R1 taken alone is methyl, ethyl or 2-hydroxyethyl; R2 taken alone is methyl, ethyl or 2-hydroxyethyl; R1 and R2 taken together are morpholino; R3 is alkyl having from about 8 to about 18 carbon atoms or R4 CONH(CH2)3 wherein R4 is alkyl having from about 8 to about 18 carbon atoms, and wherein 2-hydroxyethyl can be condensed with from 1 to about 200 units of ethylene oxide; and
(D) water, aqueous ethyl alcohol, aqueous isopropyl alcohol or an aqueous ethyl alcohol-isopropyl alcohol mixture.
21. A composition according to claim 20 wherein the amount of antimicrobial agent is from about 0.01% to about 10% by weight of the composition.
22. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 21.
23. A composition according to claim 21 wherein in the structural formula of the antimicrobial agent R is CH3 (CH2)7, Y is (CH2)10, A is Cl or Br, m is 1, n is 2 and x is 1.
24. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 23.
25. A composition according to claim 23 wherein the surfactant of part (C) is a betaine.
26. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 25.
27. A composition according to claim 23 wherein the surfactant of part (C) is an amine oxide.
28. A composition according to claim 27 wherein in the amine oxide having the structural formula ##STR35## R1 is hydroxyethyl, R2 is hydroxyethyl and R3 is the coconut radical.
29. A composition according to claim 27 wherein the polyethylene glycol surfactant of part (B) has the structural formula ##STR36## wherein ##STR37## represents the coconut acid radical and n has an average value of 78.
30. The process of reducing the number of microbes on living skin which comprises applying to the skin an antimicrobially effective amount of a composition according to claim 27.
31. An antimicrobial skin cleansing composition consisting essentially of
(A) an antimicrobially effective amount of chlorhexidine gluconate;
(B) from about 0.75% to about 30% by weight of two polyethylene glycol ester surfactants having the structural formula ##STR38## wherein ##STR39## represents the coconut acid radical, one wherein n has an average value of 78 and the other wherein n has an average value of 30;
(C) from about 0.5% to about 30% by weight of a betaine surfactant having the structural formula ##STR40## wherein R1 represents the coconut radical, R3 is 2-hydroxyethyl and R4 is .[.CH2 COO- ; and.]. .Iadd.CH2 CH2 COONa; and .Iaddend.
(D) water.
US06/752,332 1979-08-13 1985-07-03 Basic amino or ammonium antimicrobial agent-polyethylene glycol ester surfactant-betaine and/or amine oxide surfactant compositions and method of use thereof Expired - Lifetime USRE32300E (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012673A1 (en) * 1988-06-21 1989-12-28 Vax Appliances Limited Fabric cleaning and sanitizing compositions
GB2229446A (en) * 1988-06-21 1990-09-26 Vax Appliances Ltd Fabric cleaning and sanitizing compositions
EP0389648A1 (en) * 1989-03-25 1990-10-03 CWF-CHEMIE-FRANKFURT GmbH Hand decontamination and/or disinfection composition supplied by a soap dispenser
US5017617A (en) * 1988-11-22 1991-05-21 Saraya Kabushiki Kaisha Disinfectant composition for medical use
US5158763A (en) * 1990-10-09 1992-10-27 Colgate-Palmolive Company Non-staining anti-bacterial oral composition
US5180577A (en) * 1990-10-09 1993-01-19 Colgate-Palmolive Stabilized bis biguanide/anionic active ingredient compositions
US5221693A (en) * 1990-08-24 1993-06-22 The United States Of America As Represented By The Secretary Of The Department Of Health & Human Services Antimicrobial and antiviral bis-adamantanamine compounds
US5454984A (en) * 1993-04-19 1995-10-03 Reckitt & Colman Inc. All purpose cleaning composition
FR2738487A1 (en) * 1995-09-11 1997-03-14 Medix Lab ANTISEPTIC DERMATOLOGICAL COMPOSITION AND METHOD FOR PRODUCING THE SAME
US5695745A (en) * 1992-10-14 1997-12-09 The Boots Company Plc Oral hygiene composition
US5763412A (en) * 1997-04-08 1998-06-09 Becton Dickinson And Company Film-forming composition containing chlorhexidine gluconate
US6174537B1 (en) * 1998-09-25 2001-01-16 Becton, Dickinson And Company Catheter flush solution and method for its use
US7338927B2 (en) 2002-08-20 2008-03-04 Alda Pharmaceuticals Corp. Wide spectrum disinfectant comprising an alcohol and disinfectant mixture
US11021678B2 (en) * 2017-10-17 2021-06-01 Kao Corporation Liquid detergent composition for hard surfaces
US11369549B2 (en) 2017-10-12 2022-06-28 Medline Industries, Lp Antiseptic wipes

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2684924A (en) * 1951-02-05 1954-07-27 Ici Ltd Nu-chlorophenyldiguanidino compounds
US2990425A (en) * 1956-06-06 1961-06-27 Ici Ltd New biguanide salts
US3468898A (en) * 1966-05-26 1969-09-23 Sterling Drug Inc Bridged bis-biguanides and bis-guanidines
US3775477A (en) * 1971-03-10 1973-11-27 Sterling Drug Inc N,n'-bis(2-aryl-2-(hydroxy or oxo)-ethyl)-bridged-bis-carboxamides
US3855140A (en) * 1971-06-18 1974-12-17 Ici Ltd Cleansing compositions
US3867454A (en) * 1971-03-10 1975-02-18 Sterling Drug Inc 1,1-BRIDGED-BIS(N-aralkyl-2-aminoethanols)
US3940441A (en) * 1973-02-09 1976-02-24 Sterling Drug Inc. N,N'-bisphenoxybenzyl-bridged-diamides
US4022834A (en) * 1972-03-16 1977-05-10 A/S Farmaceutisk Industri Antibacterially active hexamethylene-bis-biguanides
US4045483A (en) * 1975-06-09 1977-08-30 Sterling Drug Inc. Amidinoureas and amidinothioureas
US4053636A (en) * 1976-05-24 1977-10-11 Sterling Drug Inc. Dichlorocyclopropylphenyl bisbiguanide compounds, processes and compositions
GB1533952A (en) * 1976-02-25 1978-11-29 Sterling Drug Inc Anti-microbial bis-pyridinium compounds
US4140860A (en) * 1975-06-02 1979-02-20 Sterling Drug Inc. Bridged-bis-hydroxyamide dicarbanilates

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2684924A (en) * 1951-02-05 1954-07-27 Ici Ltd Nu-chlorophenyldiguanidino compounds
US2990425A (en) * 1956-06-06 1961-06-27 Ici Ltd New biguanide salts
US3468898A (en) * 1966-05-26 1969-09-23 Sterling Drug Inc Bridged bis-biguanides and bis-guanidines
US3775477A (en) * 1971-03-10 1973-11-27 Sterling Drug Inc N,n'-bis(2-aryl-2-(hydroxy or oxo)-ethyl)-bridged-bis-carboxamides
US3867454A (en) * 1971-03-10 1975-02-18 Sterling Drug Inc 1,1-BRIDGED-BIS(N-aralkyl-2-aminoethanols)
US3855140A (en) * 1971-06-18 1974-12-17 Ici Ltd Cleansing compositions
US4022834A (en) * 1972-03-16 1977-05-10 A/S Farmaceutisk Industri Antibacterially active hexamethylene-bis-biguanides
US3940441A (en) * 1973-02-09 1976-02-24 Sterling Drug Inc. N,N'-bisphenoxybenzyl-bridged-diamides
US4140860A (en) * 1975-06-02 1979-02-20 Sterling Drug Inc. Bridged-bis-hydroxyamide dicarbanilates
US4045483A (en) * 1975-06-09 1977-08-30 Sterling Drug Inc. Amidinoureas and amidinothioureas
GB1533952A (en) * 1976-02-25 1978-11-29 Sterling Drug Inc Anti-microbial bis-pyridinium compounds
US4053636A (en) * 1976-05-24 1977-10-11 Sterling Drug Inc. Dichlorocyclopropylphenyl bisbiguanide compounds, processes and compositions

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
AROMOX Amine Oxides, Armak Co. Bulletin No. 74 21, 1974. *
AROMOX Amine Oxides, Armak Co. Bulletin No. 74-21, 1974.
CTFA Cosmetic Ingredient Dictionary, Second Edition, The Cosmetic, Toiletry and Fragrance Association, Inc., 1977, pp. 1, 24, 65 68, 95, 110, 152, 179, 203, 211, 212, 219, 255, 311. *
CTFA Cosmetic Ingredient Dictionary, Second Edition, The Cosmetic, Toiletry and Fragrance Association, Inc., 1977, pp. 1, 24, 65-68, 95, 110, 152, 179, 203, 211, 212, 219, 255, 311.
CTFA Cosmetic Ingredient Dictionary, Third Edition, The Cosmetic, Toiletry and Fragrance Association, Inc., 1982, p. 55. *
Disinfection, Sterilization and Preservation (Block), 2nd Edition, Lea & Febiger, 1977, pp. 325 360. *
Disinfection, Sterilization and Preservation (Block), 2nd Edition, Lea & Febiger, 1977, pp. 325-360.
Nonionic Surfactants (Schick), Marcel Dekker Inc., 1967, pp. 142 174. *
Nonionic Surfactants (Schick), Marcel Dekker Inc., 1967, pp. 142-174.
The Merck Index, Ninth Edition, Merck & Co., Inc., 1976: Monographs 224, 1059, 1078, 1987, 2060 and 2874. *

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2229446A (en) * 1988-06-21 1990-09-26 Vax Appliances Ltd Fabric cleaning and sanitizing compositions
WO1989012673A1 (en) * 1988-06-21 1989-12-28 Vax Appliances Limited Fabric cleaning and sanitizing compositions
US5017617A (en) * 1988-11-22 1991-05-21 Saraya Kabushiki Kaisha Disinfectant composition for medical use
EP0389648A1 (en) * 1989-03-25 1990-10-03 CWF-CHEMIE-FRANKFURT GmbH Hand decontamination and/or disinfection composition supplied by a soap dispenser
WO1990011342A1 (en) * 1989-03-25 1990-10-04 Cwf-Chemie Frankfurt Gmbh Preparation for decontaminating and/or disinfecting the hands, which can be dispensed by a soap dispenser
US5221693A (en) * 1990-08-24 1993-06-22 The United States Of America As Represented By The Secretary Of The Department Of Health & Human Services Antimicrobial and antiviral bis-adamantanamine compounds
US5158763A (en) * 1990-10-09 1992-10-27 Colgate-Palmolive Company Non-staining anti-bacterial oral composition
US5180577A (en) * 1990-10-09 1993-01-19 Colgate-Palmolive Stabilized bis biguanide/anionic active ingredient compositions
US5695745A (en) * 1992-10-14 1997-12-09 The Boots Company Plc Oral hygiene composition
US5454984A (en) * 1993-04-19 1995-10-03 Reckitt & Colman Inc. All purpose cleaning composition
US5522942A (en) * 1993-04-19 1996-06-04 Reckitt & Colman Inc. Method for cleaning hard surfaces using an aqueous solution of quaternary ammonium compound, combination of nonionic surfactant and glycol ether solvent
FR2738487A1 (en) * 1995-09-11 1997-03-14 Medix Lab ANTISEPTIC DERMATOLOGICAL COMPOSITION AND METHOD FOR PRODUCING THE SAME
WO1997009974A1 (en) * 1995-09-11 1997-03-20 Laboratoire Medix Antiseptic skin care composition and method for making same
EA000817B1 (en) * 1995-09-11 2000-04-24 Лаборатуар Медикс Anticeptic skin care composition and method for making same
US5763412A (en) * 1997-04-08 1998-06-09 Becton Dickinson And Company Film-forming composition containing chlorhexidine gluconate
US6174537B1 (en) * 1998-09-25 2001-01-16 Becton, Dickinson And Company Catheter flush solution and method for its use
US6762206B2 (en) 1998-09-25 2004-07-13 Becton, Dickinson And Company Catheter flush solution and method for its use
US7338927B2 (en) 2002-08-20 2008-03-04 Alda Pharmaceuticals Corp. Wide spectrum disinfectant comprising an alcohol and disinfectant mixture
US7560422B2 (en) 2002-08-20 2009-07-14 Alda Pharmaceuticals Corp. Alcohol-based wide spectrum disinfectant comprising nonoxynol-9
US11369549B2 (en) 2017-10-12 2022-06-28 Medline Industries, Lp Antiseptic wipes
US11021678B2 (en) * 2017-10-17 2021-06-01 Kao Corporation Liquid detergent composition for hard surfaces
US20210261881A1 (en) * 2017-10-17 2021-08-26 Kao Corporation Liquid detergent composition for hard surfaces

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