US8063357B2 - Mass spectrometer - Google Patents

Mass spectrometer Download PDF

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US8063357B2
US8063357B2 US11/571,338 US57133805A US8063357B2 US 8063357 B2 US8063357 B2 US 8063357B2 US 57133805 A US57133805 A US 57133805A US 8063357 B2 US8063357 B2 US 8063357B2
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mass
charge ratio
decimal
ions
ion
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US20110095176A1 (en
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Jose M. Castro-Perez
Alan Millar
Robert S. Plumb
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Micromass UK Ltd
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Micromass UK Ltd
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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0031—Step by step routines describing the use of the apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/20—Identification of molecular entities, parts thereof or of chemical compositions
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Definitions

  • the present invention relates to a mass spectrometer and a method of mass spectrometry.
  • metabolites of interest cannot usually be predicted. This is because the formation of metabolites may be determined by novel enzymatic reactions and by factors which are difficult to predict in advance such as bio-availability.
  • Ions which are determined as having a mass to charge ratio which indicates that they may relate to a metabolite of interest are, then fragmented in a collision cell. The resulting fragment products are then mass analysed enabling the structure of each possible metabolite to be predicted.
  • An advantage of the conventional automated mode of data acquisition is that a fair degree of data may be acquired from a single HPLC injection.
  • a disadvantage of the conventional approach is that only those peaks which have an intensity which exceeds a pre-defined intensity threshold are normally selected for subsequent MS/MS analysis (i.e. fragmentation analysis).
  • MS/MS analysis i.e. fragmentation analysis
  • Another particular problem with the conventional approach is that since the mass or mass to charge ratios of potential metabolites is not generally known in advance, then time can be wasted analysing a large number of peaks all or many of which subsequently turn out to be of little or no interest. This can also mean that actual peaks of potential interest which could have been analysed if only they had been recognised fail to be analysed at all because the mass spectrometer is busy analysing other ions.
  • a method of mass spectrometry comprising:
  • the accurate or exact mass or mass to charge ratio comprises a first integer nominal mass or mass to charge ratio component and a first decimal mass or mass to charge ratio component;
  • searching for one or more second substances or ions having a decimal mass or mass to charge ratio component which is between 0 to x 1 mDa or milli-mass to charge ratio units greater than the first decimal mass or mass to charge ratio component and/or between 0 to x 2 mDa or milli-mass to charge ratio units lesser than the first decimal mass or mass to charge ratio component.
  • the step of searching for one or more second substances or ions preferably comprises searching solely on the basis of the decimal mass or mass to charge ratio component of the one or more second substances or ions and not on the basis of the integer nominal mass or mass to charge ratio component of the one or more second substances or ions.
  • the step of searching for one or more second substances or ions preferably comprises searching some or all second substances or ions which have an integer nominal mass or mass to charge ratio component which is different from the first integer nominal mass or mass to charge ratio component.
  • x 1 falls within a range selected from the group consisting of: (i) ⁇ 1; (ii) 1-5; (iii) 5-10; (iv) 10-15; (v) 15-20; (vi) 20-25; (vii) 25-30; (viii) 30-35; (ix) 35-40; (x) 40-45; (xi) 45-50; (xii) 50-55; (xiii) 55-60; (xiv) 60-65; (xv) 65-70; (xvi) 70-75; (xvii) 75-80; (xviii) 80-85; (xix) 85-90; (xx) 90-95; (xxi) 95-100; and (xxii) >100.
  • x 2 preferably falls within a range selected from the group consisting of: (i) ⁇ 1; (ii) 1-5; (iii) 5-10; (iv) 10-15; (v) 15-20; (vi) 20-25; (vii) 25-30; (viii) 30-35; (ix) 35-40; (x) 40-45; (xi) 45-50; (xii) 50-55; (xiii) 55-60; (xiv) 60-65; (xv) 65-70; (xvi) 70-75; (xvii) 75-80; (xviii) 80-85; (xix) 85-90; (xx) 90-95; (xxi) 95-100; and (xxii) >100.
  • the first substance or ion comprises or relates to a pharmaceutical compound, drug or active component.
  • the one or more second substances or ions comprise or relate to one or more metabolites or derivatives of the first substance or ion.
  • the first substance or ion comprises a biopolymer, protein, peptide, polypeptide, oligionucleotide, oligionucleoside, amino acid, carbohydrate, sugar, lipid, fatty acid, vitamin, hormone, portion or fragment of DNA, portion or fragment of cDNA, portion or fragment of RNA, portion or fragment of mRNA, portion or fragment of tRNA, polyclonal antibody, monoclonal antibody, ribonuclease, enzyme, metabolite, polysaccharide, phosphorolated peptide, phosphorolated protein, glycopeptide, glycoprotein or steroid.
  • the one or more second substance or ion comprises a biopolymer, protein, peptide, polypeptide, oligionucleotide, oligionucleoside, amino acid, carbohydrate, sugar, lipid, fatty acid, vitamin, hormone, portion or fragment of DNA, portion or fragment of cDNA, portion or fragment of RNA, portion or fragment of mRNA, portion or fragment of tRNA, polyclonal antibody, monoclonal antibody, ribonuclease, enzyme, metabolite, polysaccharide, phosphorolated peptide, phosphorolated protein, glycopeptide, glycoprotein or steroid.
  • the sample to be analysed preferably comprises at least 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, or 5000 components, molecules or analytes having different identities or comprising different species.
  • the step of searching for one or more second substances or ions preferably further comprises applying a decimal mass or mass to charge ratio window to mass spectral data or a mass spectrum.
  • the decimal mass or mass to charge ratio window preferably filters out, removes, attenuates or at least reduces the significance of second substances or ions having a decimal mass or mass to charge ratio component which falls outside of the decimal mass or mass to charge ratio window.
  • the accurate or exact mass or mass to charge ratio of the first substance or ion minus the accurate or exact mass or mass to charge ratio of a second substance or ion preferably has a value of ⁇ M Daltons or mass to charge ratio units.
  • x 1 and/or x 2 may vary as a function of ⁇ M in a symmetrical manner.
  • x 1 and/or x 2 may vary as a function of ⁇ M in a symmetrical manner about a value of ⁇ M selected from the group consisting of: (i) 0; (ii) ⁇ 0-5; (iii) ⁇ 5-10; (iv) ⁇ 10-15; (v) ⁇ 15-20; (vi) ⁇ 20-25; (vii) ⁇ 25-30; (viii) ⁇ 30-35; (ix) ⁇ 35-40; (x) ⁇ 40-45; (xi) ⁇ 45-50; (xii) ⁇ 50-55; (xiii) ⁇ 55-60; (xiv) ⁇ 60-65; (xv) ⁇ 65-70; (xvi) ⁇ 70-75; (xvii) ⁇ 75-80; (xviii) ⁇ 80-85; (xix) ⁇ 85-90; (xx) ⁇ 90-95; (xxi) ⁇ 95-100; (xxii) >100; and (xxii)
  • x 1 and/or x 2 may vary as a function of ⁇ M in an asymmetrical manner.
  • M lower ⁇ M and/or ⁇ M ⁇ M upper then x 1 and/or x 2 has a substantially constant value.
  • M lower > ⁇ M and/or ⁇ M>M upper then x 1 and/or x 2 has a substantially non-constant value as a function of ⁇ M.
  • M lower > ⁇ M and/or ⁇ M>M upper x 1 and/or x 2 preferably varies in a substantially linear manner as a function of ⁇ M.
  • x 1 and/or x 2 preferably increases or decreases at a rate of y %* ⁇ M, wherein y is selected from the group consisting of: (i) ⁇ 0.01; (ii) 0.01-0.02; (iii) 0.02-0.03; (iv) 0.03-0.04; (v) 0.04-0.05; (vi) 0.05-0.06; (viii) 0.06-0.07; (ix) 0.07-0.08; (x) 0.08-0.09; (xi) 0.09-0.10; (xii) 0.10-0.11; (xiii) 0.11-0.12; (xiv) 0.12-0.13; (xv) 0.13-0.14; (xvi) 0.14-0.15; (xvii) 0.15-0.16; (xviii) 0.16-0.17; (xix) 0.17-0.18; (xx) 0.18-0.19; (xxi) 0.19-0.20;
  • x 1 and/or x 2 varies in a substantially curved, stepped or non-linear manner as a function of ⁇ M.
  • M upper is a value in Daltons or mass to charge ratio units and falls within a range selected from the group consisting of: (i) ⁇ 1; (ii) 1-5; (iii) 5-10; (iv) 10-15; (v) 15-20; (vi) 20-25; (vii) 25-30; (viii) 30-35; (ix) 35-40; (x) 40-45; (xi) 45-50; (xii) 50-55; (xiii) 55-60; (xiv) 60-65; (xv) 65-70; (xvi) 70-75; (xvii) 75-80; (xviii) 80-85; (xix) 85-90; (xx) 90-95; (xxi) 95-100; and (xxii) >100.
  • M lower is preferably a value in Daltons or mass to charge ratio units and falls within a range selected from the group consisting of: (i) ⁇ 100; (ii) ⁇ 100 to ⁇ 95; (iii) ⁇ 95 to ⁇ 90; (iv) ⁇ 90 to ⁇ 85; (v) ⁇ 85 to ⁇ 80; (vi) ⁇ 80 to ⁇ 75; (vii) ⁇ 75 to ⁇ 70; (viii) ⁇ 70 to ⁇ 65; (ix) ⁇ 65 to ⁇ 60; (x) ⁇ 60 to ⁇ 55; (xi) ⁇ 55 to ⁇ 50; (xii) ⁇ 50 to ⁇ 45; (xiii) ⁇ 45 to ⁇ 40; (xiv) ⁇ 40 to ⁇ 35; (xv) ⁇ 35 to ⁇ 30; (xvi) ⁇ 30 to ⁇ 25; (xvii) ⁇ 25 to ⁇ 20; (xviii) ⁇ 20 to ⁇ 15; (xix) ⁇ 15 to
  • the method further comprises selecting for further analysis one or more second substances or ions which have a decimal mass or mass to charge ratio component which is between 0 to x 1 mDa or milli-mass to charge ratio units greater than the first decimal mass or mass to charge ratio component and/or between 0 to x 2 mDa or milli-mass to charge ratio units lesser than the first decimal mass or mass to charge ratio component.
  • the step of selecting for further analysis comprises fragmenting the one or more second substances or ions.
  • the step of selecting for further analysis preferably comprises onwardly transmitting one or more second substances or ions which have a decimal mass or mass to charge ratio component which is between 0 to x 1 mDa or milli-mass to charge ratio units greater than the first decimal mass or mass to charge ratio component and/or between 0 to x 2 mDa or milli-mass to charge ratio units lesser than the first decimal mass or mass to charge ratio component to a collision or fragmentation cell.
  • the method further comprises mass analysing the fragment products or ions which result from fragmenting the one or more second substances or ions.
  • the method further comprises separating components, analytes or molecules in a sample to be analysed by means of a separation process.
  • the separation process comprises liquid chromatography.
  • the separation process may comprise: (i) High Performance Liquid Chromatography (“HPLC”); (ii) anion exchange; (iii) anion exchange chromatography; (iv) cation exchange; (v) cation exchange chromatography; (vi) ion pair reversed-phase chromatography; (vii) chromatography; (viii) single dimensional electrophoresis; (ix) multi-dimensional electrophoresis; (x) size exclusion; (xi) affinity; (xii) reverse phase chromatography; (xiii) Capillary Electrophoresis Chromatography (“CEC”); (xiv) electrophoresis; (xv) ion mobility separation; (xvi) Field Asymmetric Ion Mobility Separation or Spectrometry (“FAIMS”); (xvii
  • the method preferably further comprises ionising components, analytes or molecules in a sample to be analysed.
  • the ion source may comprise a pulsed ion source or a continuous ion source.
  • the ion source may be selected from the group consisting of: (i) an Electrospray ionisation (“ESI”) ion source; (ii) an Atmospheric Pressure Photo Ionisation (“APPI”) ion source; (iii) an Atmospheric Pressure Chemical Ionisation (“APCI”) ion source; (iv) a Matrix Assisted Laser Desorption Ionisation (“MALDI”) ion source; (v) a Laser Desorption Ionisation (“LDI”) ion source; (vi) an Atmospheric Pressure Ionisation (“API”) ion source; (vii) a Desorption Ionisation on Silicon (“DIOS”) ion source; (viii) an Electron Impact (“EI”) ion source; (ix)
  • the method further comprises mass analysing the first substance or ion and/or the one or more second substances or ions and/or fragment products or ions using a mass analyser.
  • the mass analyser preferably comprises a quadrupole mass analyser.
  • the mass analyser may comprise a mass analyser selected from the group consisting of: (i) a Fourier Transform (“FT”) mass analyser; (ii) a Fourier Transform Ion Cyclotron Resonance (“FTICR”) mass analyser; (iii) a Time of Flight (“TOF”) mass analyser; (iv) an orthogonal acceleration Time of Flight (“oaTOF”) mass analyser; (v) an axial acceleration Time of Flight mass analyser; (vi) a magnetic sector mass spectrometer; (vii) a Paul or 3D quadrupole mass analyser; (viii) a 2D or linear quadrupole mass analyser; (ix) a Penning trap mass analyser; (x) an ion trap mass analyser;
  • FT
  • the exact or accurate mass or mass to charge ratio of the first substance or ion and/or the one or more second substances or ions is preferably determined to within 20 ppm, 19 ppm, 18 ppm, 17 ppm, 16 ppm, 15 ppm, 14 ppm, 13 ppm, 12 ppm, 11 ppm, 10 ppm, 9 ppm, 8 ppm, 7 ppm, 6 ppm, 5 ppm, 4 ppm, 3 ppm, 2 ppm, 1 ppm or ⁇ 1 ppm.
  • the exact or accurate mass or mass to charge ratio of the first substance or ion and/or the one or more second substances or ions is preferably determined to within 0.01 mass units, 0.009 mass units, 0.008 mass units, 0.007 mass units, 0.006 mass units, 0.005 mass units, 0.004 mass units, 0.003 mass units, 0.002 mass units, 0.001 mass units or ⁇ 0.001 mass units.
  • the sample which is analysed according to the preferred embodiment is preferably taken from a diseased organism, a non-diseased organism, a treated organism, a non-treated organism, a mutant organism or a wild type organism.
  • the method preferably further comprises identifying or determining the composition of one or more of the second substances or ions.
  • the method further comprises quantifying or determining the intensity, concentration or expression level of the first substance or ions.
  • the method further comprises quantifying or determining the intensity, concentration or expression level of one or more of the second substances or ions.
  • the method preferably further comprises determining or quantifying the relative intensity, concentration or expression level of one or more of the first substances or ions.
  • the method further comprises determining or quantifying the relative intensity, concentration or expression level of one or more of the second substances or ions.
  • a method of mass spectrometry comprising:
  • the accurate mass to charge ratio comprises a first integer value and a first decimal value
  • searching for one or more metabolites of the parent ion wherein the step of searching comprises:
  • x falls within a range selected from the group consisting of: (i) ⁇ 1; (ii) 1-5; (iii) 5-10; (iv) 10-15; (v) 15-20; (vi) 20-25; (vii) 25-30; (viii) 30-35; (ix) 35-40; (x) 40-45; (xi) 45-50; (xii) 50-55; (xiii) 55-60; (xiv) 60-65; (xv) 65-70; (xvi) 70-75; (xvii) 75-80; (xviii) 80-85; (xix) 85-90; (xx) 90-95; (xxi) 95-100; and (xxii) >100.
  • a mass spectrometer comprising:
  • the accurate mass to charge ratio comprises a first integer value and a first decimal value
  • means arranged and adapted to search for one or more metabolites of the parent ion, wherein the means is arranged and adapted to:
  • (ii) recognise, select, preferentially mass filter or transmit, determine or fragment ions amongst the ions of potential interest on the basis of the ions having accurate mass to charge ratio wherein the second decimal value is within x mDa or milli-mass to charge ratio units of the first decimal value.
  • x falls within a range selected from the group consisting of: (i) ⁇ 1; (ii) 1-5; (iii) 5-10; (iv) 10-15; (v) 15-20; (vi) 20-25; (vii) 25-30; (viii) 30-35; (ix) 35-40; (x) 40-45; (xi) 45-50; (xii) 50-55; (xiii) 55-60; (xiv) 60-65; (xv) 65-70; (xvi) 70-75; (xvii) 75-80; (xviii) 80-85; (xix) 85-90; (xx) 90-95; (xxi) 95-100; and (xxii) >100.
  • a mass spectrometer comprising:
  • the accurate or exact mass or mass to charge ratio comprises a first integer nominal mass or mass to charge ratio component and a first decimal mass or mass to charge ratio component;
  • a method of mass spectrometry comprising searching for potential metabolites of a parent drug on the basis of the metabolites having substantially similar decimal mass or mass to charge ratios to that of the parent drug.
  • the step of searching preferably further comprises: fragmenting ions relating to a potential metabolite of a parent drug so that a plurality of fragment ions are produced; and mass analysing the fragment ions.
  • a mass spectrometer comprising means arranged and adapted to search for potential metabolites of a parent drug, wherein the means searches for ions having substantially similar decimal mass or mass to charge ratios to that of the parent drug.
  • the mass spectrometer preferably further comprises: means for fragmenting ions relating to a potential metabolite of a parent drug so that a plurality of fragment ions are produced; and means for mass analysing the fragment ions.
  • a method of mass spectrometry comprising:
  • the decimal mass or mass to charge ratio window preferably has a profile which varies as a function of ⁇ M, wherein ⁇ M is the difference in mass or mass to charge ratio between a first substance or ion and a second substance or ion.
  • the first substance or ion preferably comprises a pharmaceutical compound and the second substance or ion comprises a metabolite of the first substance or ion.
  • a mass spectrometer comprising:
  • a method of mass spectrometry comprising:
  • x is selected from the group consisting of: (i) 1; (ii) 2; (iii) 3; (iv) 4; (v) 5; (vi) 6; (vii) 7; (viii) 8; (ix) 9; (x) 10; (xi) 11; (xii) 12; (xiii) 13; (xiv) 14; (xv) 15; (xvi) 16; (xvii) 17; (xviii) 18; (xix) 19; (xx) 20; (xxi) 21; (xxii) 22; (xxiii) 23; (xxiv) 24; (xxv) 25; (xxvi) 26; (xxvii) 27; (xxviii) 28; (xxix) 29; (xxx) 30; (xxxi) 31; (xxxii) 32; (xxxii) 33; (xxxiv) 34; (xxxv) 35; (xxxvi) 36; (xxxvii) 37; (xxxxx) 30;
  • a mass spectrometer comprising:
  • liquid chromatograph arranged to subject, in use, a biological sample which includes one of metabolites of a pharmaceutical compound to liquid chromatography
  • an ion source for ionising the eluent emerging from the liquid chromatograph to produce a plurality of ions
  • ions have a mass or mass to charge ratio which has a decimal mass or mass to charge ratio component which is within x mDa or milli-mass to charge ratio units of the decimal mass or mass to charge ratio of the pharmaceutical compound.
  • x is selected from the group consisting of: (i) 1; (ii) 2; (iii) 3; (iv) 4; (v) 5; (vi) 6; (vii) 7; (viii) 8; (ix) 9; (x) 10; (xi) 11; (xii) 12; (xiii) 13; (xiv) 14; (xv) 15; (xvi) 16; (xvii) 17; (xviii) 18; (xix) 19; (xx) 20; (xxi) 21; (xxii) 22; (xxiii) 23; (xxiv) 24; (xxv) 25; (xxvi) 26; (xxvii) 27; (xxviii) 28; (xxix) 29; (xxx) 30; (xxxi) 31; (xxxii) 32; (xxxii) 33; (xxxiv) 34; (xxxv) 35; (xxxvi) 36; (xxxvii) 37; (xxxxx) 30;
  • An advantage of the preferred embodiment is that potentially only drug related metabolite peaks are selected for subsequent analysis by MS/MS and that all or at least a majority of the endogenous peaks are effectively ignored from further consideration.
  • the preferred embodiment therefore significantly improves the process of searching for and mass analysing ions relating to metabolites of interest.
  • the preferred embodiment also enables metabolites of interest to be selected for further analysis by, for example, fragmenting them within the inherent short timescales of liquid chromatography.
  • the preferred embodiment in effect, filters out or substantially removes from consideration a number of possible precursor ions for subsequent analysis by MS/MS in drug metabolism studies by selecting only those ions which have a mass or mass to charge ratio wherein the decimal part of the mass or mass to charge ratio falls within a pre-defined and preferably relatively narrow decimal mass or mass to charge ratio window.
  • FIG. 1 shows the structure and exact mass of a parent drug called Midazolam and the structure and exact mass of a hydroxylated metabolite of Midazolam;
  • FIG. 2 indicates the upper and lower limits of a decimal mass or mass to charge ratio window according to the preferred embodiment which is applied to the decimal mass or mass to charge ratio value of ions when searching mass spectral data or a mass spectrum for metabolites of a parent drug;
  • FIG. 3 shows a parent ion mass spectrum of Midazolam
  • FIG. 4 shows a parent ion mass spectrum of a hydroxylated metabolite of Midazolam
  • FIG. 5A shows the structure and exact masses of Ketotifen and Verapamil and the structure and exact masses of a metabolite of Ketotifen and Verapamil
  • FIG. 5B shows the structure and exact mass of Indinavir and the structure and exact mass of a metabolite of Indinavir.
  • FIG. 1 shows the elemental composition of a parent drug called Midazolam (C18 H13 Cl F N3) which has a monoisotopic protonated mass of 326.0860 Da.
  • a common metabolic route for the drug is the addition of oxygen. Accordingly, if an oxygen is added to Midazolem then the mass will be increased by +15.9949 Da so that the monoisotopic mass of the new compound (i.e. the hydroxylated metabolite of Midazolem) will be 342.0809 Da.
  • an ion may be assigned either an integer nominal mass or mass to charge ratio (e.g. 326 in the case of Midazolam) or an accurate or exact mass or mass to charge ratio (e.g. 326.0860 in the case of Midazolam).
  • Accurate or exact masses or mass to charge ratios can be considered as comprising an integer component or value and a decimal component or value. This largely stems from the fact that all the elements (with the exception of Carbon) have approximately but not exactly integer masses.
  • the most abundant isotope of carbon is assigned an exact atomic mass of 12.0000 Dalton (Da).
  • the accurate atomic masses of the most abundant isotopes of the most abundant elements in biological systems are Hydrogen (H) 1.0078 Da, Nitrogen (N) 14.0031 Da and Oxygen (O) 15.9949 Da.
  • Accurate or exact (i.e. non-integer) masses or mass to charge ratios can be represented as an integer nominal mass or mass to charge ratio value or component together with a corresponding mass sufficiency or deficiency value or component.
  • the mass sufficiency or deficiency may be considered to represent the deviation from an integer value and may be expressed in milli-dalton (mDa).
  • Hydrogen (H) can be expressed as having an integer nominal mass of 1 and a mass sufficiency of 7.8 mDa
  • Nitrogen (N) can be expressed as having an integer nominal mass of 14 and a mass sufficiency of 3.1 mDa
  • Oxygen (O) can be expressed as having an integer nominal mass of 16 and a mass deficiency of 5.1 mDa.
  • the mass or mass to charge ratio of an ion of an organic molecule can be assigned an integer nominal mass or mass to charge ratio together with a corresponding mass sufficiency or deficiency from that integer value.
  • the method of ionisation is also preferably taken into consideration as this allows the ionic elemental composition to be determined and hence also the ionic mass or mass to charge ratio to be calculated.
  • the analyte molecules may be protonated to form positively charged ions.
  • Metabolites are the result of bio-transformations to a parent drug.
  • An aspect of the preferred embodiment is the recognition and exploitation of the fact that the mass sufficiency or mass deficiency of a potential metabolite of interest will be substantially similar to the mass sufficiency or mass deficiency of the corresponding parent drug.
  • An aspect of the preferred embodiment is the recognition that the potential similarity between the mass sufficiency or mass deficiency of the parent ion and potential metabolites can be used to search more strategically for potential metabolites of interest.
  • the preferred embodiment searches for metabolites on the basis that the decimal part of the accurate or exact mass or mass to charge ratio of a parent drug will be substantially similar to the decimal part of the accurate or exact mass or mass to charge ratio of a metabolite of the parent drug.
  • the decimal part of the accurate mass or mass to charge ratio of a precursor ion of a parent drug is calculated.
  • a decimal mass or mass to charge ratio window is then preferably set about the precise decimal mass or mass to charge ratio of the parent drug.
  • an upper limit and a lower limit to the decimal mass window may be set.
  • only an upper limit or only a lower limit to the decimal mass window may be set.
  • the upper and lower limits may have the same magnitude or width, or alternatively the upper and lower limits may differ in magnitude or width.
  • a precursor or parent ion mass spectrum of a sample believed to contain one or more metabolites of interest is preferably obtained.
  • the parent ion mass spectrum is then preferably automatically searched for some or all mass peaks which meet the criteria that the decimal part of the accurate mass or mass to charge ratio of an ion must be very close to the decimal mass part of the accurate mass or mass to charge ratio of the known parent compound or ion.
  • ions of potential interest (which preferably relate to one or more metabolites of the parent compound) are recognised, identified or otherwise selected for further analysis by virtue of the fact that the decimal mass or mass to charge ratio of the ion is determined as falling within a relatively narrow band or range of masses or mass to charge ratios about the decimal mass or mass to charge ratio of the parent compound or ion.
  • decimal mass or mass to charge ratio window which is preferably used in the process of searching for metabolites of interest will now be described in more detail with reference to FIG. 2 .
  • FIG. 2 indicates the width of a decimal mass or mass to charge ratio window which may be used or applied according to the preferred embodiment.
  • the width of the decimal mass or mass to charge ratio window (in mDa) is shown as a function of the difference in the absolute mass (in Da) or mass to charge ratio between that of the parent ion or compound and ions or compounds being searched for which may include metabolite ions or compounds.
  • the difference in absolute mass or mass to charge ratio between the parent compound or ion and the ions or compounds being searched for, which may include metabolite ions or compounds of interest, may be referred to as ⁇ M.
  • the upper and lower limits of the decimal mass or mass to charge ratio window may be referred to as having a value ⁇ m.
  • a decimal mass or mass to charge ratio window having an upper limit +20 mDa greater than the precise decimal mass or mass to charge ratio of the parent ion and a lower limit 20 mDa below the precise decimal mass or mass to charge ratio of the parent ion may be set.
  • the upper and lower limits of the decimal mass or, mass to charge ratio window vary as a function of the absolute difference ⁇ M in the mass or mass to charge ratio of the parent ion to that of a possible metabolite ion. Therefore, as also shown in FIG. 2 , if the absolute difference in mass or mass to charge ratio between the parent ion and a potential ion of interest is say 100 Da, then according to the embodiment shown and described with reference to FIG. 2 the upper and lower limits of the decimal mass or mass to charge ratio window are asymmetric. According to the particular embodiment shown in FIG.
  • the mass or mass to charge ratio window has an upper limit +92 mDa greater than the precise decimal mass or mass to charge ratio of the parent ion and a lower limit only 50 mDa lesser than the precise decimal mass or mass to charge ratio of the parent ion.
  • the size of the upper and lower limits of the decimal mass or mass to charge ratio window may also be relatively small (e.g. in the region of 20-30 mDa).
  • the absolute difference ⁇ M in the mass or mass to charge ratio between the parent ion or compound and a possible metabolite ion or compound of interest increases, then so the size of the upper and lower limits of the decimal mass or mass to charge ratio window also preferably increases.
  • the upper limit of the decimal mass or mass to charge ratio window is preferably set to a constant value of 20 mDa. If the mass or mass to charge ratio difference between the parent ion or compound and the metabolite ion or compound of interest is >20 Da, then the upper limit of the decimal mass or mass to charge ratio window preferably increases at a rate of +0.09% times ⁇ M above 20 Da (i.e.
  • the lower limit of the decimal mass or mass to charge ratio window is preferably set to a constant value of ⁇ 20 mDa. If the mass or mass to charge ratio difference between the parent ion or compound and the metabolite ion or compound of interest is >40 Da, then the lower limit of the decimal mass or mass to charge ratio window preferably increases negatively at a rate of ⁇ 0.05% times ⁇ M above 40 Da (i.e.
  • each different parent drug will have a specific known mass or mass to charge ratio.
  • the approach according to the preferred embodiment assumes that metabolites of the parent drug will have a similar structure to that of the parent drug and that the decimal part of the accurate mass or mass to charge ratio of each metabolite will be similar to the decimal part of the accurate mass or mass to charge ratio of the parent drug.
  • Ions which according to the preferred embodiment are determined as having an accurate mass or mass to charge ratio with a decimal part which falls within the decimal mass or mass to charge ratio window as determined by the preferred embodiment are then preferably selected for further analysis by, for example, MS/MS.
  • a mass filter such as a quadrupole mass filter may be used to select specific ions which are considered to be potentially metabolite ions of interest having a specific mass to charge ratio to be onwardly transmitted to a collision or fragmentation cell. The ions are then fragmented within the collision or fragmentation cell and the resulting fragment product ions are mass analysed.
  • the preferred embodiment enables a large number of endogenous ion peaks that would otherwise have been selected for analysis by MS/MS according to the conventional approach to be automatically eliminated from consideration. This is particularly advantageous and as a result the preferred embodiment relates to a significantly improved method of recognising potential metabolites.
  • the decimal mass or mass to charge ratio window within which the decimal part of the accurate mass or mass to charge ratio of a metabolite should fall may be defined prior to proceeding with LC-MS and/or LC-MS-MS experiments.
  • the value or size of the decimal mass or mass to charge ratio window may be set to accommodate the mass errors likely to occur during an experimental run.
  • the value or size may also be set according to the elemental composition of the parent drug. For example, if the parent drug does not contain elements other than carbon, hydrogen, nitrogen, oxygen and fluorine, then the upper and/or lower limits of the decimal mass or mass to charge ratio window may be set to a lower (smaller) value than if the parent drug contains any or all of the elements phosphorous, sulphur and chlorine. This is because phosphorous, sulphur and chlorine all have larger mass deficiencies than carbon, hydrogen, nitrogen, oxygen and fluorine.
  • an asymmetric decimal mass or mass to charge ratio window may be used similar, for example, to the asymmetric decimal mass or mass to charge ratio window shown and described in relation to the embodiment depicted in FIG. 2 .
  • a simple symmetrical decimal mass or mass to charge ratio window may be used.
  • a decimal mass or mass to charge ratio window having upper and lower limits of ⁇ 20 mDa may be used. If the mass or mass to charge ratio difference between that of the parent drug and the ions of interest is ⁇ 20 Da or >20 Da then the upper and lower limits of the decimal mass or mass to charge ratio window may increase at a rate of 0.1% for mass or mass to charge ratio differences ⁇ 20 Da or >20 Da.
  • the decimal mass or mass to charge ratio window may have multiple values of decimal mass or mass to charge ratio difference ⁇ m for a mass or mass to charge ratio difference ⁇ M between that of the parent drug ions of interest.
  • the values of ⁇ m and ⁇ M may preferably be defined independently for each polarity of ⁇ m and ⁇ M.
  • the mass spectrometer is preferably capable of recording parent ion mass spectra and fragment ion mass spectra from selected precursor or parent ions that are induced to fragment.
  • the mass spectrometer may, for example, comprise a magnetic sector, a Time of Flight, an orthogonal Time of Flight, a quadrupole mass filter, a 3D quadrupole ion trap, a linear quadrupole ion trap or an FT-ICR mass analyser, or any combination thereof.
  • the mass spectrometer may comprise either a magnetic sector, a Time of Flight, an orthogonal Time of Flight or an FT-ICR mass analyser.
  • the mass spectrometer may according to an embodiment be arranged to default to the acquisition of full parent ion mass spectra unless and until a mass peak is detected wherein the decimal part of the accurate mass or mass to charge ratio of the detected ion falls within a preferably pre-defined decimal mass or mass to charge ratio window. Once such a mass peak is detected then the mass spectrometer and related control software may then preferably switch the instrument so that parent ions having a specific decimal mass or mass to charge ratio or interest are selected and transmitted by a mass filter whilst other ions having decimal masses or mass to charge ratios falling outside the decimal mass or mass to charge ratio window are preferably substantially attenuated or lost to the system.
  • Selected parent ions of interest are then preferably passed to a fragmentation or collision cell which preferably comprises an ion guide and a collision gas maintained at a pressure preferably >10 ⁇ 3 mbar.
  • the ions are preferably accelerated into the collision or fragmentation cell at energies such that upon colliding with the collision gas present in the collision or fragmentation cell, the ions are preferably caused to fragment into fragment product ions.
  • the fragment product ions are then preferably mass analysed and a full mass spectrum of the fragment product ions is then preferably obtained.
  • the size of the decimal mass or mass to charge ratio window is preferably pre-defined, according to other less preferred embodiments the size of the decimal mass or mass to charge ratio window may be altered in response to experimental data or on the basis of another parameter. According to an embodiment, for example, a first experimental run may be performed wherein a decimal mass or mass to charge ratio window having a first profile or size as a function of ⁇ M may be applied and then in a second subsequent experimental run a decimal mass or mass to charge ratio window having a second different profile or size as a function of ⁇ M may be applied.
  • control software may select or determine other parameters including the optimum fragmentation collision energy appropriate for a selected precursor or parent ion.
  • An important advantage of the preferred embodiment is that it enables more useful MS/MS spectra to be acquired within the limited timescale of a single LC-MS experiment. This reduces the time taken to get the required data.
  • Another important advantage of the preferred embodiment is that the preferred method facilitates the detection of low level metabolites that might otherwise be missed, if the conventional approach were adopted, due to the presence of a large number of relatively intense endogenous mass peaks.
  • FIG. 3 shows a parent ion mass spectrum of the drug Midazolem as recorded using a hybrid quadrupole Time of Flight mass spectrometer.
  • the measured mass to charge ratio for the major isotope was determined as being 326.0872 (cf. a theoretical value of 326.0860).
  • FIG. 4 shows a parent ion mass spectrum of the hydroxylated metabolite of Midazolam as recorded using the same hybrid quadrupole Time of Flight mass spectrometer.
  • the measured mass to charge ratio for the major isotope was determined as being 342.0822 (cf. a theoretical value of 342.0809).
  • the method according to the preferred embodiment provides an effective way of being able to detect efficiently mass peaks likely to be (or at least include) metabolites of interest with no (or relatively few) ions relating to endogenous components also being analysed.
  • the preferred method therefore advantageously effectively filters out or removes from further consideration numerous endogenous mass peaks which would otherwise have been included for consideration according to the conventional techniques.
  • the preferred embodiment advantageously enables a mass spectrometer to switch to record the fragment ion spectrum of ions which are likely to relate to metabolites of interest within the time scales during which a typical liquid chromatography mass peak is observed without wasting time analysing a large number of ions which turn out not to be metabolites of interest.
  • an intelligent exact mass deficiency algorithm may be used together with in silico metabolite prediction to predetermine DDA experiments for metabolism studies preferably using a hybrid quadrupole Time of Flight mass spectrometer.
  • specific metabolites may be predicted in advance by computer and an appropriate exact decimal mass or mass to charge ratio data filter window may be set.
  • the metabolites from a given new chemical entity or a standard compound are therefore predicted and then searched for.
  • an exact decimal mass window may be set so as to only switch to perform a DDA experiment when ions having decimal masses or mass to charge ratios within the set decimal mass or mass to charge ratio window (which may, for example, have an upper and/or lower limit of 10-20 mDa) are observed as being present.
  • a user may, for example, select or set an exact decimal mass or mass to charge ratio window to detect metabolites already predicted on the basis of their exact decimal mass or mass to charge ratio so that MS/MS experiments maybe carried out.
  • an exact mass deficiency based upon the exact mass or mass to charge ratio of the parent compound can be determined.
  • This particular data filter may be considered more specific than the data filter according to the previously described embodiment since there may be cases where not all of the metabolites will be predicted. Therefore, metabolites which are not predicted will be detected in the DDA experiments with an exact mass or mass to charge ratio data filter.
  • An exact mass or mass to charge ratio deficiency filter may operate in the following mode.
  • An exact mass or mass to charge ratio deficiency filter based upon the decimal places of the mass or mass to charge ratio of the parent drug under analysis may be used.
  • a post processing filter may be used that allows the removal of unexpected metabolite entries in a MetaboLynx browser which do not agree with user-defined criteria.
  • the use of this filter can dramatically reduce the number of false entries in an unexpected metabolite table by filtering out the vast majority of matrix-related entries which may share the same nominal mass as potential metabolites. This allows users to use low threshold values during data processing so that very low metabolite levels are identified without going through the tedious task of manually excluding false positives.
  • the filter is preferably an accurate and specific filter since it is based on exact mass and mass deficiencies which are specific to each parent drug of interest.
  • Each parent drug is comprised of a specific number of elements (C, H, N, O etc.). Depending upon the number of each one of the elements mentioned, the decimal mass or mass to charge ratio of the drug will be very specific.
  • Verapamil contains the following elements: C27 H38 N2 O4. This equates to a monoisotopic protonated mass of 455.2910 Da. If an alkyl group is taken away (N-dealkylation, a common metabolic route) and a glucuronide is added, then the mass is shifted by precisely +162.0164 Da. The metabolite therefore has a monoisotopic mass of 617.3074 Da.
  • phase II biotransformation glutathione conjugation
  • mass defect difference 68 mDa compared to the parent drug
  • most metabolites will fall within a 180 mDa decimal mass or mass to charge ratio window of the parent compound even if certain cleavages take place in the structure to yield smaller fragments.
  • FIGS. 5A and 5B show a metabolite of Ketotifen, Verapamil and Indinavir and include cleavages.
  • the maximum decimal mass or mass to charge ratio deficiency is in the case of Indinavir ( FIG. 5B ) wherein the metabolite has a decimal mass or mass to charge ratio which is 167.7 mDa different from the decimal mass or mass to charge ratio of the parent compound.
  • Mass deficiency shifts are very specific for each metabolite and parent drug.
  • the various embodiments of the present invention may be implemented not only on hybrid quadrupole orthogonal Time of Flight instruments as according to the preferred embodiment, but also using nominal mass instruments such as triple quadrupoles, linear and 3D ion traps and exact mass instruments such as MALDI/Quadrupole Time of Flight and FTMS.

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110215237A1 (en) * 2002-07-24 2011-09-08 Micromass Uk Limited Mass Analysis Using Alternating Fragmentation Modes
DE102014008264A1 (de) 2013-06-07 2014-12-11 Thermo Fisher Scientific (Bremen) Gmbh Isotopenmustererkennung

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020115056A1 (en) 2000-12-26 2002-08-22 Goodlett David R. Rapid and quantitative proteome analysis and related methods
GB0609253D0 (en) * 2006-05-10 2006-06-21 Micromass Ltd Mass spectrometer
WO2008059568A1 (fr) * 2006-11-15 2008-05-22 Shimadzu Corporation Procédé d'analyse de données de spectrométrie de masse et dispositif associé
DE102008023694B4 (de) * 2008-05-15 2010-12-30 Bruker Daltonik Gmbh Fragmentierung von Analytionen durch Ionenstoß in HF-Ionenfallen
WO2012103216A2 (en) 2011-01-25 2012-08-02 Apellis Pharmaceuticals, Inc. Apparatus and methods for assisting breathing
GB201108082D0 (en) * 2011-05-16 2011-06-29 Micromass Ltd Segmented planar calibration for correction of errors in time of flight mass spectrometers
JP5660218B2 (ja) * 2011-08-03 2015-01-28 株式会社島津製作所 質量分析データ解析方法及び装置
US9998863B2 (en) 2013-08-19 2018-06-12 Estimote Polska Sp. Z O. O. System and method for providing content using beacon systems
US10244348B2 (en) 2013-08-19 2019-03-26 Estimote Polska Sp z o.o. Methods for authenticating communication between a mobile device and wireless beacon at a remote domain name system, projecting a level of interest in a nearby product, and providing and ordering option or product data
JP6079587B2 (ja) * 2013-11-27 2017-02-15 株式会社島津製作所 糖ペプチド解析装置
US10395909B2 (en) * 2014-01-16 2019-08-27 Shimadzu Corporation Mass spectrometer
GB201407814D0 (en) * 2014-05-02 2014-06-18 Pilkington Group Ltd Glazed solar collectors
US10627407B2 (en) 2015-03-12 2020-04-21 Mars, Incorporated Ultra high resolution mass spectrometry and methods of using the same
US10136250B2 (en) 2015-09-02 2018-11-20 Estimote Polska Sp. Z O. O. System and method for lower power data routing
WO2020039252A1 (en) 2018-08-22 2020-02-27 Estimote Polska Sp Z.O.O. System and method for verifying device security
US10852441B2 (en) 2018-08-24 2020-12-01 Estimote Polska Sp z o.o. Method and system for asset management
US11239068B2 (en) * 2018-11-02 2022-02-01 Agilent Technologies, Inc. Inductively coupled plasma mass spectrometer with mass correction

Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1193255A (en) 1966-05-27 1970-05-28 Ass Elect Ind Improvements relating to Mass Spectrometry
US4008388A (en) 1974-05-16 1977-02-15 Universal Monitor Corporation Mass spectrometric system for rapid, automatic and specific identification and quantitation of compounds
US5072115A (en) 1990-12-14 1991-12-10 Finnigan Corporation Interpretation of mass spectra of multiply charged ions of mixtures
US5453613A (en) 1994-10-21 1995-09-26 Hewlett Packard Company Mass spectra interpretation system including spectra extraction
US6147344A (en) 1998-10-15 2000-11-14 Neogenesis, Inc Method for identifying compounds in a chemical mixture
US6147348A (en) 1997-04-11 2000-11-14 University Of Florida Method for performing a scan function on quadrupole ion trap mass spectrometers
US6258605B1 (en) 1999-03-26 2001-07-10 Neo Gen Screening, Inc. Clinical method for the genetic screening of newborns using tandem mass spectrometry
US6289287B1 (en) 1999-01-29 2001-09-11 Agilent Technologies, Inc. Identification of sample component using a mass sensor system
US20020063208A1 (en) 2000-11-27 2002-05-30 Surromed, Inc. Median filter for liquid chromatography-mass spectrometry data
US6455321B1 (en) 1999-01-30 2002-09-24 Neo Gen Screening, Inc. Method for interpreting tandem mass spectrometry data for clinical diagnosis
US20020192708A1 (en) 2000-10-25 2002-12-19 Hanno Steen Detection of modified amino acids by mass spectrometry
US20030023386A1 (en) 2001-01-18 2003-01-30 Nelly Aranibar Metabolome profiling methods using chromatographic and spectroscopic data in pattern recognition analysis
US20030066802A1 (en) 2001-09-18 2003-04-10 Bruker Daltonik Gmbh Method for the elucidation of metabolism
WO2003092869A1 (en) 2000-07-13 2003-11-13 The Dow Chemical Company Method for chemical analysis of biological materials
US20030229451A1 (en) 2001-11-21 2003-12-11 Carol Hamilton Methods and systems for analyzing complex biological systems
GB2394545A (en) 2001-12-08 2004-04-28 Micromass Ltd Mass spectrometry
GB2408261A (en) 2003-09-24 2005-05-25 Micromass Ltd Method of mass spectrometry of proteins
US20050272168A1 (en) * 2004-06-02 2005-12-08 Haiying Zhang Mass defect filter

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050092910A1 (en) 2001-12-08 2005-05-05 Scott Geromanos Method of mass spectrometry
US20050164325A1 (en) 2003-09-24 2005-07-28 Micromass Uk Limited Method of mass spectrometry

Patent Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1193255A (en) 1966-05-27 1970-05-28 Ass Elect Ind Improvements relating to Mass Spectrometry
US4008388A (en) 1974-05-16 1977-02-15 Universal Monitor Corporation Mass spectrometric system for rapid, automatic and specific identification and quantitation of compounds
US5072115A (en) 1990-12-14 1991-12-10 Finnigan Corporation Interpretation of mass spectra of multiply charged ions of mixtures
US5453613A (en) 1994-10-21 1995-09-26 Hewlett Packard Company Mass spectra interpretation system including spectra extraction
US6147348A (en) 1997-04-11 2000-11-14 University Of Florida Method for performing a scan function on quadrupole ion trap mass spectrometers
US6147344A (en) 1998-10-15 2000-11-14 Neogenesis, Inc Method for identifying compounds in a chemical mixture
US6289287B1 (en) 1999-01-29 2001-09-11 Agilent Technologies, Inc. Identification of sample component using a mass sensor system
US6455321B1 (en) 1999-01-30 2002-09-24 Neo Gen Screening, Inc. Method for interpreting tandem mass spectrometry data for clinical diagnosis
US6258605B1 (en) 1999-03-26 2001-07-10 Neo Gen Screening, Inc. Clinical method for the genetic screening of newborns using tandem mass spectrometry
WO2003092869A1 (en) 2000-07-13 2003-11-13 The Dow Chemical Company Method for chemical analysis of biological materials
US20020192708A1 (en) 2000-10-25 2002-12-19 Hanno Steen Detection of modified amino acids by mass spectrometry
US20020063208A1 (en) 2000-11-27 2002-05-30 Surromed, Inc. Median filter for liquid chromatography-mass spectrometry data
US20030023386A1 (en) 2001-01-18 2003-01-30 Nelly Aranibar Metabolome profiling methods using chromatographic and spectroscopic data in pattern recognition analysis
US20030066802A1 (en) 2001-09-18 2003-04-10 Bruker Daltonik Gmbh Method for the elucidation of metabolism
US20030229451A1 (en) 2001-11-21 2003-12-11 Carol Hamilton Methods and systems for analyzing complex biological systems
GB2394545A (en) 2001-12-08 2004-04-28 Micromass Ltd Mass spectrometry
GB2408261A (en) 2003-09-24 2005-05-25 Micromass Ltd Method of mass spectrometry of proteins
US20050272168A1 (en) * 2004-06-02 2005-12-08 Haiying Zhang Mass defect filter

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
Aebersold, R., et al: "Mass Spectrometry in Proteomics" Chemical Reviews, vol. 101, 2001, pp. 268-295.
Huang, R.H., et al: "Mass spectrometric strategy for primary structure determination of N-terminally blocked peptides", Journal of Chromatography B: Biomedical Sciences & Applicaitons, Elsevier, Amsterdam, NL, vol. 803, No. 1, Apr. 15, 2004, pp. 167-172.
International Application No. PCT/GB2005/002610, International Preliminary Report on Patentability and Written Opinion of the International Search Authority, Mailing Date: Jan. 9, 2007, 6 pages.
International Application No. PCT/GB2005/002610, International Search Report, Mailing Date: Dec. 21, 2006, 5 pages.
Karty, J.A., et al: "Artifacts and unassigned masses encountered in peptide mass mapping" Journal of Chromatography B, vol. 782, 2002, pp. 363-383.
Mann, M., In Useful Tables of Possible and Probable Peptide Masses, Presented at the 43rd Annual Conference on Mass Spectrometry and Allied Topics, Atlanta, GA, May 21-26, 1995; American Society for Mass Spectrometry, 1995.
Schmidt F., et al: "Iterative data analysis is the key for exhaustive analysis of peptide mass fingerprints from proteins separated by two-dimensional electrophoresis", Journal of the American Society for Mass Spectrometry, Elsevier Science Inc., NY, vol. 14, No. 9, Sep. 2003, pp. 943-956.
Translation of Notification of Reasons for Rejection for Japanese Patent Application No. 2007-519869, Dispatch Date: Apr. 14, 2011, 2 pages.
Translation of Third Party Observations regarding Japanese Patent Application No. 2007-519869, dated Dec. 5, 2008, 10 pages.
United Kingdom Patent Application No. GB0513577.7, Combined Search and Examination Report, Mailing Date: Nov. 30, 2005, 5 pages.
United Kingdom Patent Application No. GB0513577.7, Notification of Grant, Mailing Date: Jan. 9, 2007, 2 pages.
Zhang, H., et al: "A software filter to remove interference ions from drug metabolites in accurate mass liquid chromatography/mass spectrometric analyses", Journal of Mass Spectrometry, vol. 38, 2003, pp. 1110-1112 (published on-line Oct. 16, 2003).
Zhang, H., et al: "MSPurify: Leveraging High Resolution LC/MS Data to Identify Drug Metabolite Ions in Samples of Human Excreta", Proc. of the 51st ASMS Conf. on Mass Spectrometry and Allied Topics, published in the Journal of the ASMS (Oct. 2003).

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110215237A1 (en) * 2002-07-24 2011-09-08 Micromass Uk Limited Mass Analysis Using Alternating Fragmentation Modes
US8704164B2 (en) 2002-07-24 2014-04-22 Micromass Uk Limited Mass analysis using alternating fragmentation modes
US8809768B2 (en) 2002-07-24 2014-08-19 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
US9196466B2 (en) 2002-07-24 2015-11-24 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
US9384951B2 (en) 2002-07-24 2016-07-05 Micromass Uk Limited Mass analysis using alternating fragmentation modes
US9697995B2 (en) 2002-07-24 2017-07-04 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
US10083825B2 (en) 2002-07-24 2018-09-25 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
DE102014008264A1 (de) 2013-06-07 2014-12-11 Thermo Fisher Scientific (Bremen) Gmbh Isotopenmustererkennung
US10658165B2 (en) 2013-06-07 2020-05-19 Thermo Fisher Scientific (Bremen) Gmbh Isotopic pattern recognition
DE102014008264B4 (de) * 2013-06-07 2020-08-13 Thermo Fisher Scientific (Bremen) Gmbh Isotopenmustererkennung

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