US6861214B1 - Immobilization of biopolymers to aminated substrates by direct adsorption - Google Patents

Immobilization of biopolymers to aminated substrates by direct adsorption Download PDF

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Publication number
US6861214B1
US6861214B1 US09/694,701 US69470100A US6861214B1 US 6861214 B1 US6861214 B1 US 6861214B1 US 69470100 A US69470100 A US 69470100A US 6861214 B1 US6861214 B1 US 6861214B1
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Prior art keywords
target
probe
biopolymer
substrate
modified surface
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Expired - Lifetime
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US09/694,701
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Inventor
Jang B. Rampal
Robert S. Matson
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Beckman Coulter Inc
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Beckman Coulter Inc
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Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Priority to US09/694,701 priority Critical patent/US6861214B1/en
Assigned to BECKMAN COULTER, INC. reassignment BECKMAN COULTER, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: MATSON, ROBERT S., RAMPAL, JANG B.
Priority to EP01988787A priority patent/EP1337665B1/en
Priority to JP2002537918A priority patent/JP2004526420A/ja
Priority to PCT/US2001/043046 priority patent/WO2002034950A2/en
Priority to DE60126319T priority patent/DE60126319T2/de
Priority to US10/427,658 priority patent/US20040029156A1/en
Priority to US11/040,735 priority patent/US20050123986A1/en
Publication of US6861214B1 publication Critical patent/US6861214B1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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    • B01J2219/00279—Features relating to reactor vessels
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    • B01J2219/00315—Microtiter plates
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
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    • B01J2219/00635—Introduction of reactive groups to the surface by reactive plasma treatment
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US09/694,701 2000-10-23 2000-10-23 Immobilization of biopolymers to aminated substrates by direct adsorption Expired - Lifetime US6861214B1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US09/694,701 US6861214B1 (en) 2000-10-23 2000-10-23 Immobilization of biopolymers to aminated substrates by direct adsorption
DE60126319T DE60126319T2 (de) 2000-10-23 2001-10-22 Immobilisierung von biopolymeren auf aminierten substraten durch direkte adsorption
PCT/US2001/043046 WO2002034950A2 (en) 2000-10-23 2001-10-22 Immobilization of biopolymers to aminated substrates by direct adsorption
JP2002537918A JP2004526420A (ja) 2000-10-23 2001-10-22 直接的な吸着によるアミノ化基質への生体高分子の不動化
EP01988787A EP1337665B1 (en) 2000-10-23 2001-10-22 Immobilization of biopolymers to aminated substrates by direct adsorption
US10/427,658 US20040029156A1 (en) 2000-10-23 2003-05-01 Immobilization of biomolecules on substrates by attaching them to adsorbed bridging biomolecules
US11/040,735 US20050123986A1 (en) 2000-10-23 2005-01-20 Immobilization of biopolymers to aminated substrates by direct adsorption

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/694,701 US6861214B1 (en) 2000-10-23 2000-10-23 Immobilization of biopolymers to aminated substrates by direct adsorption

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/427,658 Continuation-In-Part US20040029156A1 (en) 2000-10-23 2003-05-01 Immobilization of biomolecules on substrates by attaching them to adsorbed bridging biomolecules
US11/040,735 Division US20050123986A1 (en) 2000-10-23 2005-01-20 Immobilization of biopolymers to aminated substrates by direct adsorption

Publications (1)

Publication Number Publication Date
US6861214B1 true US6861214B1 (en) 2005-03-01

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Family Applications (3)

Application Number Title Priority Date Filing Date
US09/694,701 Expired - Lifetime US6861214B1 (en) 2000-10-23 2000-10-23 Immobilization of biopolymers to aminated substrates by direct adsorption
US10/427,658 Abandoned US20040029156A1 (en) 2000-10-23 2003-05-01 Immobilization of biomolecules on substrates by attaching them to adsorbed bridging biomolecules
US11/040,735 Abandoned US20050123986A1 (en) 2000-10-23 2005-01-20 Immobilization of biopolymers to aminated substrates by direct adsorption

Family Applications After (2)

Application Number Title Priority Date Filing Date
US10/427,658 Abandoned US20040029156A1 (en) 2000-10-23 2003-05-01 Immobilization of biomolecules on substrates by attaching them to adsorbed bridging biomolecules
US11/040,735 Abandoned US20050123986A1 (en) 2000-10-23 2005-01-20 Immobilization of biopolymers to aminated substrates by direct adsorption

Country Status (5)

Country Link
US (3) US6861214B1 (ja)
EP (1) EP1337665B1 (ja)
JP (1) JP2004526420A (ja)
DE (1) DE60126319T2 (ja)
WO (1) WO2002034950A2 (ja)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020110835A1 (en) * 2001-02-13 2002-08-15 Rajan Kumar Microfluidic devices and methods
US20020142483A1 (en) * 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US20030134299A1 (en) * 2001-07-11 2003-07-17 Michael Hogan Methods and devices based upon a novel form of nucleic acid duplex on a surface
US20030148401A1 (en) * 2001-11-09 2003-08-07 Anoop Agrawal High surface area substrates for microarrays and methods to make same
US20060266958A1 (en) * 2001-05-11 2006-11-30 Fuji Photo Film Co., Ltd. Biochemical analysis data producing method and scanner used therefor
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20100261286A1 (en) * 2005-07-14 2010-10-14 Young Hoon Kim Microfluidic devices and methods of preparing and using the same
US8486717B2 (en) 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
WO2014043247A1 (en) 2012-09-12 2014-03-20 Force Diagnostics, Inc Rapid tests for insurance underwriting
US8821816B2 (en) 1997-01-23 2014-09-02 Agena Biosciences, Inc. Matrix-assisted laser desorption ionization mass spectrometry substrates having low volume matrix array elements
US9068953B2 (en) 2007-09-17 2015-06-30 Agena Bioscience, Inc. Integrated robotic sample transfer device
US9414813B2 (en) 2009-02-16 2016-08-16 Express Diagnostics Int'l, Inc. Device for assaying analytes in bodily fluids
US9599615B2 (en) 2013-03-13 2017-03-21 Symbolics, Llc Lateral flow assays using two dimensional test and control signal readout patterns
US9874556B2 (en) 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
US10888830B2 (en) 2001-07-11 2021-01-12 Genomics Usa, Inc. Methods and devices based upon a novel form of nucleic acid duplex on a surface

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003078621A1 (en) * 2002-03-13 2003-09-25 Genomics Usa Inc. Method for manufacturing microarrays based on the immobilization of porous substrates on thermally modifiable surfaces
WO2005090980A1 (ja) * 2004-03-22 2005-09-29 Olympus Corporation 生体関連物質の高感度検出方法
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US9077022B2 (en) 2004-10-29 2015-07-07 Medtronic, Inc. Lithium-ion battery
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US20130149560A1 (en) 2011-12-09 2013-06-13 Medtronic, Inc. Auxiliary electrode for lithium-ion battery
CN104897654B (zh) * 2015-06-02 2018-06-01 北京纳迅科技股份有限公司 一种微流控生物芯片检测装置及制备方法

Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588682A (en) * 1982-12-13 1986-05-13 Integrated Genetics, Inc. Binding nucleic acid to a support
US4818681A (en) 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
US4877745A (en) 1986-11-17 1989-10-31 Abbott Laboratories Apparatus and process for reagent fluid dispensing and printing
US5024933A (en) 1988-05-10 1991-06-18 Enzo Biochem, Inc. Method and kit for sample adherence to test substrate
US5112736A (en) 1989-06-14 1992-05-12 University Of Utah Dna sequencing using fluorescence background electroblotting membrane
US5215882A (en) 1989-11-30 1993-06-01 Ortho Diagnostic Systems, Inc. Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
US5445934A (en) 1989-06-07 1995-08-29 Affymax Technologies N.V. Array of oligonucleotides on a solid substrate
US5554501A (en) 1992-10-29 1996-09-10 Beckman Instruments, Inc. Biopolymer synthesis using surface activated biaxially oriented polypropylene
US5585275A (en) 1992-09-02 1996-12-17 Arris Pharmaceutical Corporation Pilot apparatus for peptide synthesis and screening
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US5622826A (en) * 1994-12-22 1997-04-22 Houston Advanced Research Center Method for immobilization of molecules on platinum solid support surfaces
US5723320A (en) 1995-08-29 1998-03-03 Dehlinger; Peter J. Position-addressable polynucleotide arrays
US5843789A (en) 1995-05-16 1998-12-01 Neomecs Incorporated Method of analysis of genomic biopolymer and porous materials for genomic analyses
US5965352A (en) 1998-05-08 1999-10-12 Rosetta Inpharmatics, Inc. Methods for identifying pathways of drug action
US5981185A (en) 1994-05-05 1999-11-09 Beckman Coulter, Inc. Oligonucleotide repeat arrays
US6013789A (en) 1998-02-20 2000-01-11 Beckman Coulter, Inc. Covalent attachment of biomolecules to derivatized polypropylene supports
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
US6197501B1 (en) * 1993-12-27 2001-03-06 Deutsches Krebsforschungszentrum Arrangement of nucleic acid sequences for comparative genomic hybridization
US6322968B1 (en) * 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777129A (en) * 1983-12-12 1988-10-11 Molecular Diagnostics, Inc. Nucleic acid probe detectable by specific nucleic acid binding protein
US4970144A (en) * 1984-12-31 1990-11-13 International Genetic Engineering Peptide fragments of human apolipoprotein, type-specific antibodies and methods of use
US5888723A (en) * 1992-02-18 1999-03-30 Johnson & Johnson Clinical Diagnostics, Inc. Method for nucleic acid amplification and detection using adhered probes
US5622626A (en) * 1994-04-15 1997-04-22 Pall Corporation Multiple compartment filter and method for processing parenteral fluid
DE69825722T2 (de) * 1998-02-04 2005-08-25 Corning Inc. Substrat zum Drucken einer Matrize

Patent Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588682A (en) * 1982-12-13 1986-05-13 Integrated Genetics, Inc. Binding nucleic acid to a support
US4818681A (en) 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
US4877745A (en) 1986-11-17 1989-10-31 Abbott Laboratories Apparatus and process for reagent fluid dispensing and printing
US5024933A (en) 1988-05-10 1991-06-18 Enzo Biochem, Inc. Method and kit for sample adherence to test substrate
US5445934A (en) 1989-06-07 1995-08-29 Affymax Technologies N.V. Array of oligonucleotides on a solid substrate
US5112736A (en) 1989-06-14 1992-05-12 University Of Utah Dna sequencing using fluorescence background electroblotting membrane
US5215882A (en) 1989-11-30 1993-06-01 Ortho Diagnostic Systems, Inc. Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
US5585275A (en) 1992-09-02 1996-12-17 Arris Pharmaceutical Corporation Pilot apparatus for peptide synthesis and screening
US5554501A (en) 1992-10-29 1996-09-10 Beckman Instruments, Inc. Biopolymer synthesis using surface activated biaxially oriented polypropylene
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US6197501B1 (en) * 1993-12-27 2001-03-06 Deutsches Krebsforschungszentrum Arrangement of nucleic acid sequences for comparative genomic hybridization
US5981185A (en) 1994-05-05 1999-11-09 Beckman Coulter, Inc. Oligonucleotide repeat arrays
US5622826A (en) * 1994-12-22 1997-04-22 Houston Advanced Research Center Method for immobilization of molecules on platinum solid support surfaces
US5843789A (en) 1995-05-16 1998-12-01 Neomecs Incorporated Method of analysis of genomic biopolymer and porous materials for genomic analyses
US5723320A (en) 1995-08-29 1998-03-03 Dehlinger; Peter J. Position-addressable polynucleotide arrays
US6322968B1 (en) * 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
US6013789A (en) 1998-02-20 2000-01-11 Beckman Coulter, Inc. Covalent attachment of biomolecules to derivatized polypropylene supports
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
US5965352A (en) 1998-05-08 1999-10-12 Rosetta Inpharmatics, Inc. Methods for identifying pathways of drug action

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Bio Techniques, "Reduction of Enzyme Adsorption to Polypropylene Surfaces in the Presence of Nonionic Detergent," vol. 17, No. 6, pp. 1048-1049, 1994.
Bradley Stevenson, et al., "Cloning of 5' cDNA Regions by Inverse PCR," Circle Reader Service No. 146, 1994.
David F. Waugh, et al., "Interactions of Bovine Thrombin and Plasma Albumin with Low-Energy Surfaces," Journal of biomdicall Materials Research, vol. 12, pp. 599-625, 1978.
L. Stanislawski, et al., "Plasma Protein Adsorption to Artificial Ligament Fibers," Journal of Biomedical Materials Research, vol. 29, pp. 315-323, 1995.
R. Ganapathy, et al., "Immobilization of alpha-Chymotrypsin on Oxygen-RF-Plasma Functionalized PET and PP Surfaces," J. Biomater. Sci. Polymer Edn., vol. 9, No. 4, pp. 389-404, 1998.
R. Sipehia, et al., "Immobilization of Enzymes on Polypropylene Bead Surfaces by Anhydrous Ammonia Gaseous Plasma Technique," Journal of Biomedical Materials Research, vol. 22, pp. 417-422, 1988.
Ronald C. Chatelier, et al., "Covalent Attachment and Non-Specific Binding of Reactive Probe Molecules onto Surfaces," J. Biomater Sci. Polymer Edn., vol. 7, pp. 601-622, 1995.

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US20020142483A1 (en) * 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US20060024841A1 (en) * 2000-10-30 2006-02-02 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US9669376B2 (en) 2000-10-30 2017-06-06 Agena Bioscience, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US8999266B2 (en) 2000-10-30 2015-04-07 Agena Bioscience, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US20020110835A1 (en) * 2001-02-13 2002-08-15 Rajan Kumar Microfluidic devices and methods
US20060266958A1 (en) * 2001-05-11 2006-11-30 Fuji Photo Film Co., Ltd. Biochemical analysis data producing method and scanner used therefor
US7170078B2 (en) * 2001-05-11 2007-01-30 Fuji Photo Film Co., Ltd. Biochemical analysis data producing method and scanner used therefor
US20030134299A1 (en) * 2001-07-11 2003-07-17 Michael Hogan Methods and devices based upon a novel form of nucleic acid duplex on a surface
US10888830B2 (en) 2001-07-11 2021-01-12 Genomics Usa, Inc. Methods and devices based upon a novel form of nucleic acid duplex on a surface
US7354710B2 (en) * 2001-07-11 2008-04-08 Genomics Usa, Inc. Methods and devices based upon a novel form of nucleic acid duplex on a surface
US7195872B2 (en) * 2001-11-09 2007-03-27 3D Biosurfaces, Inc. High surface area substrates for microarrays and methods to make same
US20030148401A1 (en) * 2001-11-09 2003-08-07 Anoop Agrawal High surface area substrates for microarrays and methods to make same
US20100261286A1 (en) * 2005-07-14 2010-10-14 Young Hoon Kim Microfluidic devices and methods of preparing and using the same
US9068953B2 (en) 2007-09-17 2015-06-30 Agena Bioscience, Inc. Integrated robotic sample transfer device
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20100285479A1 (en) * 2008-02-22 2010-11-11 Great Basin Scientific Systems and methods for point-of-care amplification and detection of polynucleotides
US8637250B2 (en) 2008-02-22 2014-01-28 Great Basin Scientific Systems and methods for point-of-care amplification and detection of polynucleotides
US9414813B2 (en) 2009-02-16 2016-08-16 Express Diagnostics Int'l, Inc. Device for assaying analytes in bodily fluids
US9462998B2 (en) 2009-02-16 2016-10-11 Express Diagnostics Int'l, Inc. Device for assaying analytes in bodily fluids
US10076314B2 (en) 2009-02-16 2018-09-18 Express Diagnostics Int'l, Inc. Device for assaying analytes in bodily fluids
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