US5106840A - Method of preparing n-oxo-tetrahydro-beta-carbolines - Google Patents
Method of preparing n-oxo-tetrahydro-beta-carbolines Download PDFInfo
- Publication number
- US5106840A US5106840A US07/533,415 US53341590A US5106840A US 5106840 A US5106840 A US 5106840A US 53341590 A US53341590 A US 53341590A US 5106840 A US5106840 A US 5106840A
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- US
- United States
- Prior art keywords
- carbon atoms
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- formula
- compound
- hydrogen
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D515/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
- C07D515/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains three hetero rings
- C07D515/14—Ortho-condensed systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Definitions
- the invention relates to a new method of preparing known and new N-oxo-tetrahydro- ⁇ -carbolines.
- alkaloids of the tetrahydro- ⁇ -carboline type for example, yohimbine, vincamine and corynantheine, have interesting pharmacological properties (ref. 1).
- eudistamins tetrahydro-N-oxocarboline compounds
- eudistamins show a strong antiviral activity with respect to Simplex- and polio virus (ref. 2); in addition they have an antitumour activity (ref. 3).
- eudistamins have been isolated in very small quantities from biological material (ref. 2) and have been obtained via a total synthesis in a very low yield (ref. 4).
- n has the value 0, 1 or 2
- R 2 is hydrogen, lower alkyl or acyl
- R 3 is hydrogen, lower alkyl or alkoxycarbonyl, or a phenyl group optionally substituted with a group R 1 , and
- groups having 1-4 C-atoms are denoted by alkyl or alkoxy, alkylthio, lower alkyl, alkoxy or alkylthio.
- Group Z in the starting compounds of formula 2 for the intramolecular cyclisation reaction according to the invention is a group which is capable of performing a double electrophilic attack on both the aliphatic nitrogen atom and the carbon atom of the indole ring.
- it relates notably to aldehyde groups and acetal groups and on the other hand to ester groups.
- the so-called Pictet-Spengler cyclisation occurs under the influence of an acid catalyst, for example, trifluoroacetic acid.
- a reductor for example, diisobutyl aluminum hydride (DIBAL) is also used to generate the required level of oxidation at the C-atom of the group Z.
- DIBAL diisobutyl aluminum hydride
- the starting compounds of formula 2 can be obtained by reaction of a compound of formula 3 ##STR5## with a compound of formula 4 ##STR6## in which formulae R 1 , n, R 2 , R 3 , A and Z have the above-mentioned meanings, and X is a so-called leaving group, preferably halogen or a sulphonate group.
- the compounds of formula 4 can be obtained in a manner known for the preparation of this type of compounds.
- Examples of good protecting groups for the nitrogen atom are acyl or alkoxycarbonyl, for example, trichloroethoxycarbonyl or 2-(trimethylsilyl) ethoxycarbonyl.
- the protecting group may be removed in a manner known per se, for example, as described in (ref. 6).
- the compounds of formula 1 are new compounds with the exception of a number of eudistamin derivatives of formula 5 ##STR7## wherein a) m and n are 0, and R 4 is hydrogen or tert.butoxycarbonyl (Boc), or
- n 1, m is 0 or 1, R 1 is 11--Br and R 4 is hydrogen or acetyl, or
- n 1, m is 0, R 1 is 10--Br and R 4 is hydrogen or Boc, or
- the process according to the invention is particularly suitable for the stereo-selective total synthesis of both new and known eudistamin derivatives.
- the processes used so far (ref. 4) are characterised by an inversion of the sequence in which the functional groups in compounds of the formulae 3 and 4 are coupled to each other.
- this results in complete racemisation at carbon atom C-1 in the first reaction step (i.e. an intermolecular Pictet-Spengler reaction), and/or gives extremely low yields in the second step (i.e. formation of a thermodynamically unfavourable 7-membered ring).
- a further aspect of the invention is a new method for the preparation of eudistamins wherein R 4 is hydrogen starting with the corresponding compound wherein R 4 is Boc.
- the known method (ref. 4) with a strong acid (trifluoroacetic acid, TFA) is very inefficient and results in extensive degradation of the desired product.
- TFA trifluoroacetic acid
- the invention also relates to the new compounds of formula 1 and the salts thereof.
- the invention relates to the various diastereo isomers and enantiomers, and to (racemic) mixtures thereof.
- Compounds having formula 1 have interesting farmacological properties. Similar to some known eudistomins also the new compounds having formula 5 have strong antiviral and antitumour activity. It has surprisingly been found that compounds nos. 63 and 67 have a very broad spectrum of antiviral activity in 22 antiviral test models. This is unique in comparison with known antiviral agents, such as for example acyclofur, ribavirin and other nucleoside analogs. Moreover, new compound no. 67 has been found to have at least ten times higher potency in comparison with known compound 63, which is found in nature.
- the compounds of formula 1 and suitable salts thereof may be brought into forms suitable for administration, for example, pills, tablets, coated tablets, capsules, powders, injection liquids and the like while using techniques and auxiliary substances conventionally used for this purpose.
- TrOC trichloroethyloxycarbonyl
- DIBAL diisobutyl aluminum hydride
- Compound 47b Crystalline from EtOAc/n-hexane; melting-point 145°-147° C.
- DIBAL (1N, 3 ml) in toluene was added dropwise, while stirring, to a cooled (-70° C.) solution of compound 37 (390 mg, 1.4 mmol) in dry toluene (75 ml) under an atmosphere of argon. After stirring for 2 hours the excess of DIBAL was destroyed at -70° C. by carefully adding dropwise TFA (5 eq.) in toluene, after which the reaction mixture was poured in ice-water (100 ml). The organic layer was separated and the neutralised aqueous layer was washed twice with 100 ml of EtOAc. The combined organic layer was washed with a brine solution and dried with MgSO 4 . Evaporation of the solvent provided crystalline 48, which was purified by means of column chromatography (CHCl 3 /MeOH, 99/1). Yield 241 mg (76%) of 48, melting-point 178°-180° C.
- the antitumour activity of some eudistomin derivatives and the reference antitumour drug was assessed in an in-vitro test with high predictive power for in-vivo activity.
- the inhibition of P 388 leukemia cells colony formation was determined at several concentrations of the test compounds as described in reference 8. The activity is expressed as an ID 50 value, which indicates the dose causing 50% inhibition with respect to untreated control cells. The following data were found:
- Antiviral activity of some eudistomin derivatives was determined in a large number of in-vitro assays as described in reference 9. These tests are all based on the inhibition of virus induced cytopathogenicity. Depending upon the virus studied, different cell cultures were used. As a general rule it is accepted that a compound is considered as antivirally active when the minimal inhibition concentration (MIC-value) is at least ten-fold lower than the minimal cytotoxic concentration (MCC-value).
- MCC-value minimal cytotoxic concentration
- the results for compounds 50, 63 en 67 together with ribavirin as a standard are given in the Tables B-E. For both compound 63 and especially 67 an exceptionally wide range of activities in the 22 antiviral assays is found. New compound 67 has the additional advantage of a 10-fold higher potency as compared with known compound 63.
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Indole Compounds (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/016,675 US5348949A (en) | 1989-06-08 | 1993-02-12 | Method of preparing N-oxo-tetrahydro-β-carbolines |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL8901458 | 1989-06-08 | ||
NL8901458 | 1989-06-08 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US85150792A Division | 1989-06-08 | 1992-03-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
US5106840A true US5106840A (en) | 1992-04-21 |
Family
ID=19854798
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/533,415 Expired - Fee Related US5106840A (en) | 1989-06-08 | 1990-06-05 | Method of preparing n-oxo-tetrahydro-beta-carbolines |
US08/016,675 Expired - Fee Related US5348949A (en) | 1989-06-08 | 1993-02-12 | Method of preparing N-oxo-tetrahydro-β-carbolines |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/016,675 Expired - Fee Related US5348949A (en) | 1989-06-08 | 1993-02-12 | Method of preparing N-oxo-tetrahydro-β-carbolines |
Country Status (8)
Country | Link |
---|---|
US (2) | US5106840A (de) |
EP (2) | EP0401929A3 (de) |
JP (1) | JPH0368582A (de) |
AU (1) | AU634550B2 (de) |
CA (1) | CA2018253A1 (de) |
IL (1) | IL94645A0 (de) |
NZ (1) | NZ233940A (de) |
ZA (1) | ZA904311B (de) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06102822A (ja) * | 1991-09-26 | 1994-04-15 | Rooreru Intelligent Syst:Kk | ファイルセキュリティシステム |
WO2005082373A1 (ja) * | 2004-02-27 | 2005-09-09 | Mitsubishi Pharma Corporation | ユージストミン誘導体医薬組成物 |
EP1683521A1 (de) * | 2005-01-21 | 2006-07-26 | Centre National De La Recherche Scientifique (Cnrs) | Peptid-Deformylase-Inhibitoren, deren Verwendung und pharmazeutische Zusammensetzungen, welche sie enthalten |
WO2006088191A1 (ja) * | 2005-02-21 | 2006-08-24 | Mitsubishi Pharma Corporation | ユージストミン誘導体 |
US20210179605A1 (en) * | 2018-07-27 | 2021-06-17 | University Of Sharjah | Discovery of Novel First in Class Nature-Inspired Compounds Targeting the Mitochondrial Function and Pharmaceutical Composition Thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4631149A (en) * | 1983-07-25 | 1986-12-23 | University Of Illinois | Antiviral eudistomins from a marine tunicate |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
HU194220B (en) * | 1985-04-19 | 1988-01-28 | Richter Gedeon Vegyeszet | Process for production of derivatives of 1,12 b disubstituated-octahydro-indolo /2,3-a/ quinolisine and medical compounds containing thereof |
-
1990
- 1990-06-05 ZA ZA904311A patent/ZA904311B/xx unknown
- 1990-06-05 JP JP2145552A patent/JPH0368582A/ja active Pending
- 1990-06-05 EP EP19900201430 patent/EP0401929A3/de not_active Withdrawn
- 1990-06-05 CA CA002018253A patent/CA2018253A1/en not_active Abandoned
- 1990-06-05 NZ NZ233940A patent/NZ233940A/xx unknown
- 1990-06-05 EP EP96200625A patent/EP0719777A3/de not_active Withdrawn
- 1990-06-05 US US07/533,415 patent/US5106840A/en not_active Expired - Fee Related
- 1990-06-06 IL IL94645A patent/IL94645A0/xx unknown
- 1990-06-07 AU AU56891/90A patent/AU634550B2/en not_active Ceased
-
1993
- 1993-02-12 US US08/016,675 patent/US5348949A/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4631149A (en) * | 1983-07-25 | 1986-12-23 | University Of Illinois | Antiviral eudistomins from a marine tunicate |
Non-Patent Citations (6)
Title |
---|
J. Am. Chem. Soc. 109, 3378, (1987), and Tetrahedron Letters, 29, 2255 (1988). * |
J. Chem. Soc. Comm. 1979, 495, and J. Org. Chem. 44, 1247 (1979). * |
J. Chem. Soc., Chem. Comm. 1979 514. * |
J. Org. Chem., 47, 2147 (1982), and J. Chem. Soc. (1965) 7179. * |
Tetrahedron Letters 28, 4971 (1988), and Austr. J. Chem 42, 1201 (1989). * |
Tetrahedron Letters, 30, 1041, (1989), and J. Am. Chem. Soc. 11, 2721 (1989). * |
Also Published As
Publication number | Publication date |
---|---|
EP0401929A3 (de) | 1991-10-23 |
AU634550B2 (en) | 1993-02-25 |
EP0401929A2 (de) | 1990-12-12 |
US5348949A (en) | 1994-09-20 |
CA2018253A1 (en) | 1990-12-08 |
EP0719777A2 (de) | 1996-07-03 |
EP0719777A3 (de) | 1996-07-17 |
ZA904311B (en) | 1991-04-24 |
AU5689190A (en) | 1990-12-13 |
NZ233940A (en) | 1993-04-28 |
JPH0368582A (ja) | 1991-03-25 |
IL94645A0 (en) | 1991-04-15 |
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Legal Events
Date | Code | Title | Description |
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AS | Assignment |
Owner name: DUPHAR INTERNATIONAL RESEARCH B.V., NETHERLANDS Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:HERMKENS, PETER H. H.;VAN MAARSEVEEN, JAN H.;SCHEEREN, JOHAN W.;AND OTHERS;REEL/FRAME:005433/0653;SIGNING DATES FROM 19900713 TO 19900808 |
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CC | Certificate of correction | ||
FEPP | Fee payment procedure |
Free format text: PAYOR NUMBER ASSIGNED (ORIGINAL EVENT CODE: ASPN); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
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FPAY | Fee payment |
Year of fee payment: 4 |
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REMI | Maintenance fee reminder mailed | ||
LAPS | Lapse for failure to pay maintenance fees | ||
FP | Lapsed due to failure to pay maintenance fee |
Effective date: 20000421 |
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STCH | Information on status: patent discontinuation |
Free format text: PATENT EXPIRED DUE TO NONPAYMENT OF MAINTENANCE FEES UNDER 37 CFR 1.362 |