US3863599A - Applicator means - Google Patents

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Publication number
US3863599A
US3863599A US435722A US43572274A US3863599A US 3863599 A US3863599 A US 3863599A US 435722 A US435722 A US 435722A US 43572274 A US43572274 A US 43572274A US 3863599 A US3863599 A US 3863599A
Authority
US
United States
Prior art keywords
applicator
strands
strand
substrate
spaced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US435722A
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English (en)
Inventor
Joachim Kohn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandon Southern Instruments Ltd
Original Assignee
Shandon Southern Instruments Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandon Southern Instruments Ltd filed Critical Shandon Southern Instruments Ltd
Application granted granted Critical
Publication of US3863599A publication Critical patent/US3863599A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0845—Filaments, strings, fibres, i.e. not hollow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0244—Drop counters; Drop formers using pins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/54—Supports specially adapted for pipettes and burettes
    • B01L9/543—Supports specially adapted for pipettes and burettes for disposable pipette tips, e.g. racks or cassettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers

Definitions

  • An applicator for forming a colinear array of spaced liquid sample streaks on a substrate for conducting parallel electrophoretic or chromatographic runs on such samples comprises a body supporting an array of aligned applicating strands of, eg, stainless steel foil. in a manner enabling the individual strands to be dipped in liquid samples to pick-up a coating of liquid on each to be transferred to a substrate.
  • the strands are preferably individually resiliently supported on the body so as to be self-aligning with the surface of a substrate against which the strands are pressed.
  • the applicator is an adaptation of that of U.S. Pat. No. 3,505,858 to overcome the special difficulties in forming reproducible streaks of certain sample liquids, particularly haemoglobin extracted from whole blood.
  • the invention relates to applicators and is specifically concerned with an applicator for forming a colinear array of spaced liquid sample streaks on a substrate.
  • the substrate is normally prepared for conducting a set of parallel runs by forming streaks of the different samples on the surface at one end of the substrate.
  • the streaks are normally arranged in a colinear array extending at right angles to the direction in which constituents of the streaks are to travel during the runs.
  • the different constituents travel from the streaks to form discrete bands. To ensure spatial separation, in the direction of travel, of the bands of constituents which travel at only slightly differing speeds it is important that the streaks which constitute the origins of the runs should have negligible width.
  • Kohn U.S. Pat. No. 3505858 discloses an applicator for the purposes discussed above which avoids the difficulties in use and the disadvantages of the above-described simple parallel wire applicator, the applicator the subject of the said Patent comprising an elongated plate-like body provided in one longitudinal edge thereof with a plurality of cutouts which separate said longitudinal edge into a plurality of longitudinally spaced discrete castellations, said castellations having elongated edge-elements disposed in longitudinal alignment, said edge-elements being provided with liquid-holding channels of uniform crosssection extending longitudinally in the edge-elements.
  • Applicators constructed in accordance with the said Patent have proved very convenient and simple to use and their use has been found to be conducive to the easy obtainment of highly reproducible results in the various analytical techniques involving their use.
  • the edge-elements are intended to reproduce, functionally, the spaced parallel wires of the simple previously proposed applicator discussed above.
  • the liquid-holding channels in the edgeelements are broadly equivalent in function to the gap between the spaced parallel wires of the simple applicator and retain liquid by capillarity.
  • the channels may be formed by grooves in the face of elements of the applicating edge or they may be constituted by gaps formed between spaced pairs of parallel members defining the edge-elements.
  • the edge-elements and their liquid-holding channels of an applicator constructed as taught by the said Patent serve reliably to pick-up a reasonably standard quantity of sample liquid and to transfer this to a substrate against which the applicating edge is lightly touched.
  • sample liquids notably haemoglobin extracted from whole blood
  • An object of the present invention is, therefore, to provide a modification of the applicator of the said U.S. Pat. No. 3505858 which, for the purposes indicated, avoids at least substantially the abovediscussed performance limitations of the applicator of the said Patent.
  • An applicator in accordance with the present invention is based upon the concept of utilising a single linear strand, such as a wire or a thin, narrow strip, as an edge-element, it having been found that in the case of the sample liquids under consideration, such as haemoglobin, an isolated linear strand when dipped into such a liquid will pick-up a coating that is substantially uniform along the length of the strand and will release such coating or a proportion thereof uniformly to a substrate against which the strand is lightly pressed.
  • An applicator in accordance with the present invention thus comprises a body and supporting an array of colinear spaced-apart applicating strands of similar dimension spaced from the body and freely suspended therefrom by support members engaging solely the ends of the strands.
  • each applicating strand is formed as a part of an integral assembly including a pair of end support members by which the strand is attached to the said body.
  • each said strand may consist of the reduced-width central portion of the length of an elongated strip, the end portions of which are bent parallel to one another at right angles to the strand-constituting central portion.
  • Conveniently integral strands and support member assemblies of this form are made of stainless steel strip.
  • each assembly of strand and end support members may be arranged for individual mounting on the said body so as to allow for positional adjustment of individual assemblies and for replacement of damaged assemblies should this become necessary.
  • the body may comprise a stainless steel or similar blade which may or may not be provided with finger pieces or other means to facilitate its handling, this strip being fitted adjacent to one long edge thereof with a mounting block formed with pairs of apertures to receive, as a push fit, the end support members of integral strand and support member assemblies.
  • the mounting block may conveniently be formed of plastics material, for instance methylmethacrylate or the like, and desirably the apertures therein consist of through-bores to facilitate thorough cleansing of the individual strands and their support members.
  • the said body comprises an elongated rectangular section block of, e.g., a suitable plastics material having a rectangular slot to receive a set'of mounting blocks each fitted with an individual strand and its end support members, the mounting blocks being a sliding fit in the body and abutting against resilient means therein so that relative movement of the mounting blocks may occur to align the several strands in the plane of a substrate when pressed thereagainst.
  • a suitable plastics material having a rectangular slot to receive a set'of mounting blocks each fitted with an individual strand and its end support members, the mounting blocks being a sliding fit in the body and abutting against resilient means therein so that relative movement of the mounting blocks may occur to align the several strands in the plane of a substrate when pressed thereagainst.
  • the support members may have barbed or serrated edges.
  • the dimensions of the strands of an applicator in accordance with the invention determine its performance and, in particular, the amount of liquid which such a strand will pick-up and transfer to a substrate; moreover, the nature of the liquid will affect the amount thereof picked-up and transferred by a strand of given dimensions. It has been found that for haemoglobin extracted from whole blood, a strand formed from stainless steel strip having a thickness of 0.005 inch and a width of 0.010 inch is suitable, a strand of length 0.200 inch between support members at right angles thereto being suitable to transfer appropriate amounts of haemoglobin samples to a substrate to form a streak of negligible width thereon.
  • FIG. 1 is a front threequarter perspective view of an applicator, in accordance with a preferred embodiment of the invention, with parts broken away to show internal details;
  • FIG. 2 is a rear threequarter perspective view of the applicator, on a reduced scale.
  • FIG. 3 is a plan view of a blank from which an integral strand and support assembly is formed for the applicator of FIGS. 1 and 2.
  • the applicator shown in the drawing comprises a body 1 in the form of a block of elongated rectangular section having a rectangular slot 2 formed by a recess in one large face of the block and extending into one narrow face thereof, so that for most of its length the block 1 is of inverted L" section.
  • the recessed large face of the block I is covered by a mainplate 3 fixed to the block by means of setscrews 4.
  • the mainplate 3 has a flange which covers the recessed narrow face of the block 1 and is slotted for a purpose to be described.
  • the block 1 may be formed of metal or a suitable plastics material, e.g., polymethylmethacrylate, and the mainplate 3 may be formed of stainless steel.
  • a set of mounting blocks 5 is accommodated within the slot 2 in the block I, the blocks 5 being rectangular and dimensioned to be a sliding fit in the slot 2 defined by the recess and the mainplate 3.
  • Each mounting block 5 has an individual spring 6 acting between the base of the slot and the block 5 to urge the latter towards the recessed narrow face of the block 1 and into abutment with the inner face of the flange of the mainplate 3.
  • Each mounting block 5 is fitted with a strand assembly comprising a stainless steel ribbon-like strand 7 integrally formed with a pair of end support members 8 extending parallel with one another and at right angles to the plane and length of the strand 7.
  • Each strand assembly is formed by bending a blank of the configuration shown in FIG. 3.
  • the blank which is conveniently a stamping of stainless steel foil 0.005 inch thick, has a central portion constituting the strand 7 with a length of, e.g., 0.200 inch and a width of 0.010 inch and end portions constituting the end support members 8, the said end portions each having an overall length of about 0.400 inch and a width which increases over a distance of about 0.100 inch from the strand width of 0.010 inch to a nominal width of about 0.070 inch with sets of barbs 9 having an overall width of about 0.095 inch.
  • Each mounting block 5 has transverse slots (not shown) into which the barbed ends of the end support members 8 are pushed so that these members 8 support the strand 7 of the associated assembly substantially parallel with the recessed narrow face of the block 1.
  • the slots in the flange of the mainplate 3 are disposed in register with the respective strand assemblies so that each projects through the mainplate flange.
  • a spring-loaded guard 10 of channel section with a slotted web straddles the recessed narrow face of the block 1 and overlies the strand assemblies projecting therefrom.
  • the guard 10 is secured by plungers ll movable in bores in the block 1, springs 12 seating in counterbores and surrounding the plungers ll acting against the inside face of the guard 10 to urge this away from the block 1 into a position in which it encloses the strand assemblies.
  • the mainplate 3 is fitted with finger pieces 13 by means of which the applicator may be manipulated.
  • the rear face of the block 1 is fitted with dowels l4 and a headed peg 15 by means of which the applicator may, if desired, be fitted to a holder, for instance the end of an arm hinged over a base plate having a movable table.
  • the illustrated applicator may be used in generally the same manner as the applicator of the said US. Pat. No. 3505858. Thus it may be used in conjunction with a pallet comprising a rectangular flat dish with a line of stations formed by recesses in one face, these recesses corresponding in size and position to the strands of the applicator so that the applicator strands may be introduced into the recesses simultaneously, each to pick-up a sample ofliquid from the individual recess into which it is introduced. With its strands charged with samples. the applicator may then be moved into light contact with a substrate so that each strand deposits a sample as a thin line on the substrate.
  • the guard 10 encloses the strands and protects these from inadvertent contamination or damage.
  • the springs 6 of the mounting blocks 5 enable these to move as required for the strands 7 to align with a substrate when pressed thereagainst.
  • An applicator for forming on a substrate a number of spaced colinear liquid streaks each having substan' tially the same volume of liquid per unit length. comprising a body, an array of colinear spaced-apart liquidholding and applicating strands of similar dimension spaced from the body, and support members freely suspending the strands from the body and engaging solely the ends of the strands.
  • each said strand consists of the reduced-width central portion of the length of an elongated strip, the end portions of which are bent parallel to one another at right angles to the strand-constituting central portion.
  • said body comprises an elongated rectangular section block having a rectangular slot, a set of mounting blocks received in said slot, each said mounting block being fitted with an individual strand and end support member assembly, the mounting blocks being a sliding tit in the body and abutting against resilient means therein.
  • the applicator of claim 5 including a mainplate having a flange overlying said slot to retain said mounting blocks therein and itself slotted to permit the strands to extend through said flange.
  • the applicator of claim 6, including a springloaded guard overlying said strands and displacable to expose these for application to a substrate.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Processing Of Stones Or Stones Resemblance Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
US435722A 1973-01-23 1974-01-23 Applicator means Expired - Lifetime US3863599A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB343173A GB1448581A (en) 1973-01-23 1973-01-23 Applicators

Publications (1)

Publication Number Publication Date
US3863599A true US3863599A (en) 1975-02-04

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ID=9758216

Family Applications (1)

Application Number Title Priority Date Filing Date
US435722A Expired - Lifetime US3863599A (en) 1973-01-23 1974-01-23 Applicator means

Country Status (3)

Country Link
US (1) US3863599A (enrdf_load_stackoverflow)
JP (1) JPS49106892A (enrdf_load_stackoverflow)
GB (1) GB1448581A (enrdf_load_stackoverflow)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4006705A (en) * 1975-10-08 1977-02-08 Helena Laboratories Corporation Limited pressure applicator
FR2372428A1 (enrdf_load_stackoverflow) * 1976-11-24 1978-06-23 Nasa
US4096825A (en) * 1975-10-08 1978-06-27 Helena Laboratories Corporation Apparatus for applying an organic liquid sample
US5405516A (en) * 1991-01-04 1995-04-11 Sebia Apparatus for the application of biological samples to an electrophoretic slab support
US5464515A (en) * 1991-01-04 1995-11-07 Sebia, A Corp. Of France Apparatus for the application of biological samples to an electrophoretic slab support
US20110088572A1 (en) * 2009-10-21 2011-04-21 Keiichi Morishita Machining oil applying apparatus

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50137793A (enrdf_load_stackoverflow) * 1974-04-20 1975-11-01

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US65948A (en) * 1867-06-18 Henby e
US2656605A (en) * 1952-02-11 1953-10-27 Leland F Schlieder Chalk holder or the like
US3505858A (en) * 1966-11-25 1970-04-14 Shandon Scient Co Ltd Applicator means

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US65948A (en) * 1867-06-18 Henby e
US2656605A (en) * 1952-02-11 1953-10-27 Leland F Schlieder Chalk holder or the like
US3505858A (en) * 1966-11-25 1970-04-14 Shandon Scient Co Ltd Applicator means

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4006705A (en) * 1975-10-08 1977-02-08 Helena Laboratories Corporation Limited pressure applicator
US4096825A (en) * 1975-10-08 1978-06-27 Helena Laboratories Corporation Apparatus for applying an organic liquid sample
FR2372428A1 (enrdf_load_stackoverflow) * 1976-11-24 1978-06-23 Nasa
US5405516A (en) * 1991-01-04 1995-04-11 Sebia Apparatus for the application of biological samples to an electrophoretic slab support
US5464515A (en) * 1991-01-04 1995-11-07 Sebia, A Corp. Of France Apparatus for the application of biological samples to an electrophoretic slab support
US20110088572A1 (en) * 2009-10-21 2011-04-21 Keiichi Morishita Machining oil applying apparatus
US8875626B2 (en) * 2009-10-21 2014-11-04 Hidaka Seiki Kabushiki Kaisha Machining oil applying apparatus

Also Published As

Publication number Publication date
JPS49106892A (enrdf_load_stackoverflow) 1974-10-09
GB1448581A (en) 1976-09-08

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