US3801004A - Device for collecting the contents of cuvets in a rotating spectrophotometer analyzer - Google Patents

Device for collecting the contents of cuvets in a rotating spectrophotometer analyzer Download PDF

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Publication number
US3801004A
US3801004A US00291282A US3801004DA US3801004A US 3801004 A US3801004 A US 3801004A US 00291282 A US00291282 A US 00291282A US 3801004D A US3801004D A US 3801004DA US 3801004 A US3801004 A US 3801004A
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US
United States
Prior art keywords
ring
cuvet
cuvets
liquid
rotating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00291282A
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English (en)
Inventor
S Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adaltis US Inc
Original Assignee
Union Carbide Corp
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Publication date
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Assigned to BAKER INSTRUMENTS CORPORATION, A CORP. OF DE reassignment BAKER INSTRUMENTS CORPORATION, A CORP. OF DE ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: UNION CARBIDE CORPORATION
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes

Definitions

  • the invention relates to a device for collecting liquid from the cuvets of a rotating spectrophotometer.
  • Reagents are placed in the inner cavities, and the samples to be analyzed are placed in cavities at a greater radial distance than those containing the reagents.
  • the sample loading disc is then indexed and positioned in the rotor with each individual reagent and sample system having a corresponding cuvet.
  • centrifugal force moves the reagent to the cavity containing the sample, where they are mixed and the mixture of reagent and sample is then moved by centrifugal force through a communicating passage into the cuvet.
  • the filled cuvets rapidly spin past the fixed light beam, and the transmission of light through the cuvets, i.e., through the samples, is measured.
  • FIG. 1 shows an elevational cross-section view of a rotating photometric analyzer incorporating the collecting device of the present invention.
  • FIG. 2 is a partial plan view of a sample disc and cuvet ring of a rotating photometric analyzer
  • FIG 3 shows an isometric view of portion of the collecting device of the present invention.
  • FIG. 1 shows in elevation a rotating photometric analyzer comprising a removable sample-reagent disc 10 supported on a rotor 11 and suitably indexed thereto (not shown).
  • Rotor 11 is affixed to drive assembly 12 by pin 15.
  • Drive assembly 12 is rotatably driven by way of v-belt pulley 13 attached to drive shaft 17.
  • the portion 16 of rotor 11 is suitably of stainless steel over which is arranged a glass plate 18.
  • the body portion 20 of sample-reagent disc 10 is suitably formed of Teflon (Trademark of E. I. Dupont de Nemours) and contains a plurality, usually about 30, of concentrically arranged inter-connecting cavities indicated at 22.
  • Reagent cavity 24 contains for example a glucose reagent such as a 0.3 molar triethanolamine buffer of pH 7.5 containing 0.0004 Mol/- liter NADP, 0.0005 Mol/liter ATP, mg/liter hexokinase, 140 mg/liter glucose-6-phosphate dehydrogenase and 0.004 Mol/liter MgSO
  • the reagent, indicated at 26, upon spinning of the sample-reagent disc 10, passes into sample cavity 28, containing for example blood serum, and both liquids pass via transfer passage 30 into Cuvette 32 located in ring member 34 which is affixed (not shown) to rotor 11.
  • Cuvette 32, and all other cuvettes, are enclosed by ring member 34.
  • Ring member 34 is suitably formed of aluminum. The extent of the reaction between the liquids in cuvette 32 is measured photometrically by means of light source 38 and photomultiplier 40 in accordance with known techniques.
  • the rotating photometric analyzer is substantially enclosed by metal housing 48, plastic ring shield member 51 and plastic cap member 52, which encloses sample reagent disc 10 containing cavities 22.
  • a collecting ring 50 suitably made of aluminum if fixedly attached to cuvet ring member 34 and is rotatable therewith upon actuation of drive assembly 12.
  • pressurized gas e.g., forced air
  • conduit 60 into passageway 64 of drive assembly 12, conduits 66 in enclosed sample-reagent disc 10.
  • the forced air is used to move cuvet liquid 33, previously confined within cuvets 32 and siphon arms 80, to siphon arms 82. Due to the siphon arrangements 68, communicating via conduits 70 with cuvets 32, the cuvet liquid 33 moves to the outer surface of cuvet ring 34.
  • the forced air forces the cuvet liquid to the outer surface of the cuvet ring 34 where centrifugal force developed by rotation of the cuvet ring 34 causes the expelled cuvet liquid 71 to be thrown against the generally concave surface 72 of annular collecting ring 50 and held by centrifugal force in a relatively narrow and confined zone.
  • the cuvet liquid 71 falls by gravity over restraining edge 75 into drain ring 77 from which it can be conveniently withdrawn via conduit 79 utilizing conventional pumping mechanisms.
  • the air space in the annular passage is essentially static with respect to the expelled cuvet liquid since collecting ring 50 and cuvet ring 34 rotate together.
  • Spattering is further minimized by arranging the collecting ring 50 such that the expelled cuvet liquid strikes the collecting ring at acute angle to the tangent of the collecting ring surface as indicated at 92.
  • the device of the present invention is self-balanced in both the empty and filling conditions. Further, the device of the present invention is uncomplicated, inexpensive and requires no maintenance.
  • a rotating spectrophotometric chemical analyzer including a rotating cuvet ring having an outer surface and having cuvettes in communication with said outer surface, said cuvets being adapted to contain liquid
  • the improvement for collecting liquid from the cuvets which comprises means for forcing the liquid in said cuvets to the outer surface of said rotating cuvet ring to enable the centrifugal force developed by said cuvet ring to separate said liquid from said cuvet ring and collecting means fixedly positioned with respect to said rotating cuvet ring and rotating therewith having a generally concave inner surface opposite said cuvets arranged to restrain and confine liquid separated from 2.
  • a rotating spectrophotometric chemical analyzer including a rotating cuvet ring having an outer surface and having cuvettes in communication with said outer surface, said cuvets being adapted to contain liquid
  • the improvement for collecting liquid from the cuvets which comprises conduit means communicating with each of said cuvets and the outer surface of said cuvet ring and arranged whereby liquid is retained in said cuvets and said conduit means; means for applying pressurized gas to said cuvets to force the liquid in said cuvets through said conduits to the outer surface of said rotating cuvet ring to enable the centrifugal force developed by said cuvet ring to separate said liquid from said cuvet ring; and an annular collecting member affixed to and surrounding said cuvet ring having a generally concave inner surface arranged to restrain and confine the liquid separated from said cuvet ring within said annular collecting during rotation of said cuvet ring.
  • an additional collecting member is located below said annular ring member to receive liquid from said annular ring by gravity flow when said annular ring member ceases to rotate.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
US00291282A 1972-09-22 1972-09-22 Device for collecting the contents of cuvets in a rotating spectrophotometer analyzer Expired - Lifetime US3801004A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29128272A 1972-09-22 1972-09-22

Publications (1)

Publication Number Publication Date
US3801004A true US3801004A (en) 1974-04-02

Family

ID=23119675

Family Applications (1)

Application Number Title Priority Date Filing Date
US00291282A Expired - Lifetime US3801004A (en) 1972-09-22 1972-09-22 Device for collecting the contents of cuvets in a rotating spectrophotometer analyzer

Country Status (6)

Country Link
US (1) US3801004A (enrdf_load_stackoverflow)
JP (1) JPS542875B2 (enrdf_load_stackoverflow)
CH (1) CH572610A5 (enrdf_load_stackoverflow)
DE (1) DE2347465C3 (enrdf_load_stackoverflow)
FR (1) FR2200513B1 (enrdf_load_stackoverflow)
GB (1) GB1438133A (enrdf_load_stackoverflow)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870014A (en) * 1973-05-24 1975-03-11 Corning Glass Works Liquid and aerosol collecting device
US4030834A (en) * 1976-04-08 1977-06-21 The United States Of America As Represented By The United States Energy Research And Development Administration Dynamic multistation photometer
DE3049050A1 (de) * 1979-12-28 1981-10-15 Hitachi Koki Co. Ltd., Katsuta, Ibaragi Verfahren und vorrichtung zum getrennten sammeln und entleeren von fluessigkeitsteilen in einer zentrifuge
US4557600A (en) * 1981-09-01 1985-12-10 Boehringer Mannheim Gmbh Process and device for the control and mixing of a fluid current subjected to centrifugal force

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1161138B (it) * 1983-04-13 1987-03-11 Instrumentation Lab Spa Fotometro analitico, di tipo centrifugo, atto alla determinazione praticamente simultanea della presenza di differenti sostanze in un certo numero di campioni discreti
DE3425009A1 (de) * 1984-07-06 1986-02-06 Boehringer Mannheim Gmbh, 6800 Mannheim Vorrichtung zum durchspuelen einer in einem feld befindlichen substanz mit einem fluessigen spuelmittel in mehreren spuelvorgaengen und zur ueberfuehrung des beim letzten spuelvorgang aus dem feld austretenden spuelmittels in eine kuevette

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2688437A (en) * 1947-12-04 1954-09-07 Saint Gobain Centrifugal separator
US3119775A (en) * 1959-09-10 1964-01-28 Westfalia Separator Ag Centrifugal separator construction for separating curds from whey
US3244363A (en) * 1959-06-24 1966-04-05 George N Hein Centrifuge apparatus and bag therefor
US3547547A (en) * 1969-03-13 1970-12-15 Atomic Energy Commission Analytical photometer with means for measuring,holding and transferring discrete liquid volumes and method of use thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1184352A (en) * 1966-04-12 1970-03-18 Beckman Instruments Inc Cell Identification and Selection System for Centrifuge Apparatus
US3555284A (en) * 1968-12-18 1971-01-12 Norman G Anderson Multistation, single channel analytical photometer and method of use
JPS5023624A (enrdf_load_stackoverflow) * 1973-07-02 1975-03-13

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2688437A (en) * 1947-12-04 1954-09-07 Saint Gobain Centrifugal separator
US3244363A (en) * 1959-06-24 1966-04-05 George N Hein Centrifuge apparatus and bag therefor
US3119775A (en) * 1959-09-10 1964-01-28 Westfalia Separator Ag Centrifugal separator construction for separating curds from whey
US3547547A (en) * 1969-03-13 1970-12-15 Atomic Energy Commission Analytical photometer with means for measuring,holding and transferring discrete liquid volumes and method of use thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870014A (en) * 1973-05-24 1975-03-11 Corning Glass Works Liquid and aerosol collecting device
US4030834A (en) * 1976-04-08 1977-06-21 The United States Of America As Represented By The United States Energy Research And Development Administration Dynamic multistation photometer
DE3049050A1 (de) * 1979-12-28 1981-10-15 Hitachi Koki Co. Ltd., Katsuta, Ibaragi Verfahren und vorrichtung zum getrennten sammeln und entleeren von fluessigkeitsteilen in einer zentrifuge
US4343709A (en) * 1979-12-28 1982-08-10 Tetsuo Matsumoto Method and device for separately collecting and discharging components of liquid in centrifugal rotor
US4557600A (en) * 1981-09-01 1985-12-10 Boehringer Mannheim Gmbh Process and device for the control and mixing of a fluid current subjected to centrifugal force

Also Published As

Publication number Publication date
FR2200513A1 (enrdf_load_stackoverflow) 1974-04-19
FR2200513B1 (enrdf_load_stackoverflow) 1976-06-18
DE2347465C3 (de) 1978-04-13
DE2347465B2 (de) 1977-07-21
GB1438133A (en) 1976-06-03
JPS4971986A (enrdf_load_stackoverflow) 1974-07-11
DE2347465A1 (de) 1974-04-11
CH572610A5 (enrdf_load_stackoverflow) 1976-02-13
JPS542875B2 (enrdf_load_stackoverflow) 1979-02-14

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AS Assignment

Owner name: BAKER INSTRUMENTS CORPORATION, 2196 AVENUE C, P.O.

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:UNION CARBIDE CORPORATION;REEL/FRAME:003865/0890

Effective date: 19810512