US3773626A - Reagent composition and process for determining gamma-glutamyltranspeptidase - Google Patents

Reagent composition and process for determining gamma-glutamyltranspeptidase Download PDF

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Publication number
US3773626A
US3773626A US00170968A US3773626DA US3773626A US 3773626 A US3773626 A US 3773626A US 00170968 A US00170968 A US 00170968A US 3773626D A US3773626D A US 3773626DA US 3773626 A US3773626 A US 3773626A
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US
United States
Prior art keywords
grams
glutamyl
nitroaniline
composition
gamma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00170968A
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English (en)
Inventor
E Bernt
H Bergmeyer
W Gruber
W Lippert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
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Boehringer Mannheim GmbH
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Filing date
Publication date
Priority claimed from DE19702042829 external-priority patent/DE2042829C/de
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
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Publication of US3773626A publication Critical patent/US3773626A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Definitions

  • ABSTRACT A composition comprising -glutamyl-p-nitroaniline, glucyl-glycine, a buffer, a surface-active agent and polyvinyl-pyrrolidone is an exceptionally stable reagent for determining 'y-glutamyl-transpeptidase.
  • y-GT 'y-glutarnyl-transpeptidase
  • the present invention substantially overcomes this drawback of the prior art compositions by providing a reagent which does not exhibit the above described instability.
  • the present invention is based upon the surprising discovery that the use of polyvinyl-pyrrolidone, together with a surface-active agent, substantially and decisively improves the solubility properties and the stability of 'y-glutamyl-p-nitroaniline.
  • a reagent composition for the determination of 'y-glutamyl-transpeptidase which comprises -y-glutamyl-p-nitroaniline, glycyl-glycine and a buffer, together with polyvinyl-pyrrolidone and a surface-active agent.
  • the reagent according to the present invention preferably also contains an azide, especially an alkali metal azide, for example sodium azide.
  • the reagent according to the present invention can contain any such agent which does not exert an inhibiting or inactivating influence on the -y-glutamyl-transpeptidase.
  • the hydroxypolyethoxyalkanes, especially hydroxypolyethoxydodecane, have proved to be especially useful.
  • the agent according to the present invention preferably contains the 'y-glutamyl-p-nitroaniline in lyophilized form, optionally together with the other components of the reagent in solid form. More preferably, however, the reagent according to the present invention consists of two mixtures, one of which contains 'y-glutamyl-p-nitroaniline and glycyl-glycine in lyophilized form and the other consists of an aqueous solution, which contains the buffer, polyvinyl-pyrrolidone, the surface-active agent and optionally an azide. Both of the mixtures are stable for a long time and, by simple mixing, with gentle warming, permit the rapid preparation of a relatively stable solution, which remains usable for a comparatively long time.
  • the aqueous solution comprises:
  • the aqueous solution comprises:
  • the second component of the reagent according to the present invention i.e., the solid mixture of y-glutamyl-p-nitroaniline and glycyl-glycine, preferably comprises:
  • the reagent according to the present invention permits the preparation of solutions ready to use for the determination of y-glutamyl-transpeptidase, which solutions remain usable for a comparatively long period of time, in contradistinction to the previously known solutions of 'y-glutamyl-p-nitroaniline which, on the one hand, were difficult to prepare and from which, on the other hand, this compound crystallized out again a short time after preparation of the solution.
  • the reagent according to the present invention provides the possibility of carrying out the routine determination of 'y-glutamyl-transpeptidase, for example, in automatic analysis devices.
  • Reagent composition for the determination of 'y-glutamyl-transpeptidase which composition comprises y-glutamyl-p-nitroaniline, glycyl-glycine, a tris buffer, a hydroxypolyethoxyalkane surface-active agent and polyvinylpyrrolidone.
  • composition as claimed in claim 1 additionally containing an azide.
  • composition as claimed in claim 2 wherein said azide is an alkali metal azide.
  • composition as claimed in claim 1 wherein the surface-active agent is an hydroxypolyethoxydodecane.
  • composition as claimed in claim 1 wherein the buffer is a tris -(hydroxyalkylamine).
  • composition as claimed in claim 1 comprising:
  • composition as claimed in claim 1 comprising:
  • composition as claimed in claim 6 wherein the azide is sodium azide.
  • Method for determining 'y-glutamyl-transpeptidase which comprises contacting a test sample suspected of containing y-glutamyl-transpeptidase with a diagnostic reagent composition comprising 'y-glutamyl-pnitroaniline, glycyl-glycine, a tris buffer, a hydroxypolyethoxyalkane surface-agent and polyvinylpyrrolidone, and following the liberation of pnitroaniline as a measure of any activity of 'y-glutamyltranspeptidase present in the test sample.
  • a diagnostic reagent composition comprising 'y-glutamyl-pnitroaniline, glycyl-glycine, a tris buffer, a hydroxypolyethoxyalkane surface-agent and polyvinylpyrrolidone, and following the liberation of pnitroaniline as a measure of any activity of 'y-glutamyltranspeptidase present in the test
  • Method as claimed in claim 9 which comprises forming a reagent composition by contacting, per liter of solution,
  • Method as claimed in claim 10 which comprises forming a reagent composition by contacting, per liter of solution,

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
US00170968A 1970-08-28 1971-08-11 Reagent composition and process for determining gamma-glutamyltranspeptidase Expired - Lifetime US3773626A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19702042829 DE2042829C (de) 1970-08-28 Reagens zur Bestimmung der gamma Glutamyltranspeptidase

Publications (1)

Publication Number Publication Date
US3773626A true US3773626A (en) 1973-11-20

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Family Applications (1)

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US00170968A Expired - Lifetime US3773626A (en) 1970-08-28 1971-08-11 Reagent composition and process for determining gamma-glutamyltranspeptidase

Country Status (10)

Country Link
US (1) US3773626A (cg-RX-API-DMAC10.html)
AT (1) AT308050B (cg-RX-API-DMAC10.html)
CA (1) CA939597A (cg-RX-API-DMAC10.html)
FI (1) FI51639C (cg-RX-API-DMAC10.html)
FR (1) FR2106163A5 (cg-RX-API-DMAC10.html)
GB (1) GB1288699A (cg-RX-API-DMAC10.html)
IL (1) IL37325A (cg-RX-API-DMAC10.html)
IT (1) IT961512B (cg-RX-API-DMAC10.html)
NL (1) NL174373C (cg-RX-API-DMAC10.html)
SE (1) SE386277B (cg-RX-API-DMAC10.html)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3878048A (en) * 1972-08-21 1975-04-15 Warner Lambert Co Determination of gamma-glutamyl transpeptidase in biological fluids and diagnostic reagents used therefor
US3892631A (en) * 1972-08-21 1975-07-01 Warner Lambert Co Diagnostic reagents used for the determination of gamma-glutamyl transpeptidase in biological fluids
US3986931A (en) * 1972-12-05 1976-10-19 Boehringer Mannheim G.M.B.H. γ-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING γ-GLUTAMYL TRANSPEPTIDASE
US4049702A (en) * 1972-12-05 1977-09-20 Boehringer Mannheim G.M.B.H. γ-Glutamyl-4-nitroanilide compounds
US4167449A (en) * 1976-07-29 1979-09-11 American Hospital Supply Corporation Composition and method for determining transferase and protease activity
US4321364A (en) * 1980-04-17 1982-03-23 Minister For Public Works For The State Of New South Wales Preparation of soluble chromogenic substrates
US4425427A (en) 1980-03-13 1984-01-10 Vitafin N.V. Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components
US4448715A (en) * 1981-11-02 1984-05-15 University Of Miami Tagged pyroglu-L-Phe-L-Arg derivatives, substrates and assays for kallikrein
US4511651A (en) * 1982-07-30 1985-04-16 American Monitor Corporation Reagent composition and assay for the determination of γ-glutamyltransferase activity
US5506114A (en) * 1992-02-07 1996-04-09 Osborn Laboratories Methods and kits for detecting the presence or concentration of biological analytes
WO2001012653A1 (en) * 1999-08-17 2001-02-22 Novo Nordisk A/S Stabilisation of freeze-dried cake
US6586574B1 (en) 1999-08-17 2003-07-01 Nn A/S Stabilization of freeze-dried cake

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2917999A1 (de) * 1979-05-04 1980-11-13 Behringwerke Ag Substrat zur bestimmung der gama -glutamyltranspeptidase
DE3370821D1 (en) * 1982-08-30 1987-05-14 Beckman Instruments Inc Method for determining _g(g)-glutamyltransferase activity and kits containing a novel substrate solution for use therein

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3878048A (en) * 1972-08-21 1975-04-15 Warner Lambert Co Determination of gamma-glutamyl transpeptidase in biological fluids and diagnostic reagents used therefor
US3892631A (en) * 1972-08-21 1975-07-01 Warner Lambert Co Diagnostic reagents used for the determination of gamma-glutamyl transpeptidase in biological fluids
US3986931A (en) * 1972-12-05 1976-10-19 Boehringer Mannheim G.M.B.H. γ-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING γ-GLUTAMYL TRANSPEPTIDASE
US4049702A (en) * 1972-12-05 1977-09-20 Boehringer Mannheim G.M.B.H. γ-Glutamyl-4-nitroanilide compounds
US4167449A (en) * 1976-07-29 1979-09-11 American Hospital Supply Corporation Composition and method for determining transferase and protease activity
US4425427A (en) 1980-03-13 1984-01-10 Vitafin N.V. Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components
US4321364A (en) * 1980-04-17 1982-03-23 Minister For Public Works For The State Of New South Wales Preparation of soluble chromogenic substrates
US4448715A (en) * 1981-11-02 1984-05-15 University Of Miami Tagged pyroglu-L-Phe-L-Arg derivatives, substrates and assays for kallikrein
US4511651A (en) * 1982-07-30 1985-04-16 American Monitor Corporation Reagent composition and assay for the determination of γ-glutamyltransferase activity
US5506114A (en) * 1992-02-07 1996-04-09 Osborn Laboratories Methods and kits for detecting the presence or concentration of biological analytes
WO2001012653A1 (en) * 1999-08-17 2001-02-22 Novo Nordisk A/S Stabilisation of freeze-dried cake
US6586574B1 (en) 1999-08-17 2003-07-01 Nn A/S Stabilization of freeze-dried cake

Also Published As

Publication number Publication date
IL37325A (en) 1975-02-10
FR2106163A5 (cg-RX-API-DMAC10.html) 1972-04-28
FI51639B (cg-RX-API-DMAC10.html) 1976-11-01
NL7111669A (cg-RX-API-DMAC10.html) 1972-03-01
DE2042829B2 (de) 1972-11-09
SE386277B (sv) 1976-08-02
NL174373B (nl) 1984-01-02
FI51639C (fi) 1977-02-10
DE2042829A1 (de) 1972-03-16
IL37325A0 (en) 1971-10-20
NL174373C (nl) 1984-06-01
IT961512B (it) 1973-12-10
GB1288699A (cg-RX-API-DMAC10.html) 1972-09-13
AT308050B (de) 1973-06-25
CA939597A (en) 1974-01-08

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