Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
  • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
  • Y10T436/108331—Preservative, buffer, anticoagulant or diluent
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
  • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]

Definitions

• ABSTRACT Test composition for determining ascorbic acid in fluids and the relative concentration of ascorbic acid present therein.
• the composition includes a solution containing an indicator stablized with a nitrate compound.
• the composition may be used as a spot test or incorporated with a carrier such as a bibulous material and dried to form a dip and read! test device.
• An organic acid may also be included in the composition to prevent urate interference and adapt the composition for testing urine specimens.
• a dip and read test device consisting of paper impregnated with ammonium phosphomolybdate has been reported for determining the presence of ascorbic acid in fluids.
• the reported test device is inconvenient to prepare in that it requires a double irnpregnation of the bibulous paper, first with phosphomolybdic acid and then with an ammonium salt for the purpose of preparing ammonium phosphomolybdate and a separate drying step after each impregnation is necessary.
• the device is reported to be stable for only a few days. Also, to adapt the prior art device to testing urine specimens, it is first necessary to acidify the specimen to be tested with a strong mineral acid prior to testing in order to lower the pH of the specimen and thereby avoid urate interference.
• test composition of thisinvention is designed to overcome the stability and preparation problems encountered in the prior art and obviate the need to acidify the specimen to overcome the urate Interference possibility commonly associated with testing urine by providing an integral test solution.
• test composition to determine the concentration of ascorbic acid in fluids comprising one or more phosphomolybdate salts and a soluble nitrate salt.
• the test composition may further include an organic acid to adapt the composition for urinalysis.
• the composition indicator is preferably one or more phosphomolybdate salts which provide a noticeable color change in the presence of ascorbic acid. This color change is known in the art to be caused by a re duction of the phosphomolybdate salt to molybdenum blue. It has been found that the indicator in the composition must be present in a concentration of at least 0.1 percent by weight and preferably from about 0.1 percent to about 0.6 percent by weight.
• Sodium phosphomolybdate salts have been found to be acceptable for use as an indicator in the composition. It has been found that equal quantities of sodium 2-phospho-l8-molybdate and sodium phospho-IZ- molybdate are preferred since such a combination provides both a rapid reaction in the presence of ascorbic acid and an intense color change.
• nitrate salts such as potassium or sodium nitrate, are acceptable for this purpose.
• composition is readily adaptable for use as a reagent for conducting spot tests for the presence and concentration of ascorbic acid in fluids to be analyzed.
• procedure for conducting spot tests is well known in the art and particular applications of the test composition of this invention to this type of test' would prove to be verbose.
• composition is also conducive for incorporation with a carrier for dip and read type of devices.
• a carrier for dip and read type of devices for this purpose, an appropriate bibulous material or carrier is chosen, such as filter paper, wood, cloth or the like.
• the bibulous material is impregnated by saturating the material with the composition.
• the saturated material is then dried at approximately C to about C to evaporate or remove the aqueous ve hicle of the composition.
• the dip and read test devices may then be cut to a workable and economical size and stored until needed for use.
• the composition may also be incorporated with polymeric semi-permeable membranes or matrices to form a paperless dip and read device.
• the thusly-prepared test device may also be coated with a semi-permeable membrane such as ethyl cellulose to adapt the device. for testing whole blood.
• a semi-permeable membrane such as ethyl cellulose
• the presence of any ascorbic acid in the test fluid will cause the indicator present in the test composition or test device to turn a blue color.
• the intensity of the color is directly proportional to the concentration or amount of ascorbic acid present in the test sample.
• a color chart may be provided to facilitate interpretation of the test results.
• EXAMPLE I To 100.0 ml. water, 0.2 gm. of sodium 2-phospho-l 8- molybdate, 0.2 gm. of sodium phospho-l2-molybdate and 2.0 gm. of sodium nitrate were added and thoroughly mixed until dissolved. Five sheets of E&D No. 204 filter paper were then separately immersed into the above solution until saturated. The sheets were removed from the impregnating solution and placed in a forced air oven and dried at about 100C for about minutes.
• the dried, impregnated sheets were observed to be off-white to light yellow in color.
• the sheets were then cut into strips and placed within moisture-proof, lightresistant bottles for testing or storage.
• solutions were prepared containing 40 mg percent, 120 mg percent, 240 mg percent and 400 mg percent of ascorbic acid in water. A control containing only water was also tested.
• test devices were dipped into the above solutions and the following color changes in the test devices were observed after approximately 60 seconds:
• EXAMPLE ill Test devices prepared in accordance with Examples I and II, only absent the sodium nitrate, were prepared. These devices and those of Examples l and II were placed in ovens set at 60 and C. After 24 hours, the test devices were examined and no change was observed in the sodium nitrate treated devices while a color reaction was observed in the untreated devices.
• test devices of Examples I and II were exposed to air at room temperature. Upon observation after exposure to air after 48 hours, the devices containing the sodium nitrate had not changed while the test devices lacking the sodium nitrate stabilizer had changed to a bluish-green color. The stabilized test devices were again observed at weekly intervals for four weeks and no change was observed.
• test compositions and device for determining the concentration of ascorbic acid in fluids have thus been described which are both simple to use, accurate and stable. Additionally, the composition and test device are simple to prepare and use, the test device re quiring only a single impregnation and thereby providing an economical test. Likewise to incorporate a compound to prevent urate interference provides an integral testing composition or device adaptable for testing urine.
• test composition to determine the presence and concentration of ascorbic acid in fluids comprising:
• an aqueous solution containing one or more sodium phosphomolybdate salts and a neutral nitrate salt containing one or more sodium phosphomolybdate salts and a neutral nitrate salt.
• test composition as defined in claim 1 wherein the solution contains from about 0.1 percent to about 0.6 percent by weight of the phosphomolybdate salts.
• test device for determining the presence of ascorbic acid in fluids comprising a dry carrier material incorporated with the composition of claim 1.

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Molecular Biology (AREA)
• Biomedical Technology (AREA)
• Chemical & Material Sciences (AREA)
• Hematology (AREA)
• Immunology (AREA)
• Urology & Nephrology (AREA)
• Cell Biology (AREA)
• Microbiology (AREA)
• Biotechnology (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Physics & Mathematics (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Pathology (AREA)
• Investigating Or Analysing Biological Materials (AREA)
• Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Applications Claiming Priority (1)

Publications (1)

Family

ID=22863806

Family Applications (1)

Country Status (13)

Cited By (9)

Families Citing this family (1)

• 1972
  • 1972-02-28 US US00230062A patent/US3771964A/en not_active Expired - Lifetime
  • 1972-10-20 CA CA154,380A patent/CA955158A/en not_active Expired
  • 1972-10-26 AU AU48154/72A patent/AU463018B2/en not_active Expired
  • 1972-11-22 GB GB5397572A patent/GB1386140A/en not_active Expired
• 1973
  • 1973-02-20 ZA ZA731184A patent/ZA731184B/xx unknown
  • 1973-02-26 HU HUMI524A patent/HU165715B/hu unknown
  • 1973-02-27 FR FR7306889A patent/FR2174595A5/fr not_active Expired
  • 1973-02-27 JP JP2275173A patent/JPS53712B2/ja not_active Expired
  • 1973-02-27 AR AR246819A patent/AR194783A1/es active
  • 1973-02-27 ES ES412118A patent/ES412118A1/es not_active Expired
  • 1973-02-27 SE SE7302763A patent/SE381102B/xx unknown
  • 1973-02-27 DE DE2309794A patent/DE2309794C3/de not_active Expired
  • 1973-02-27 BR BR731462A patent/BR7301462D0/pt unknown

Non-Patent Citations (4)

Also Published As

Similar Documents