US3736100A - Immunoelectrophoresis gel tray - Google Patents

Immunoelectrophoresis gel tray Download PDF

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Publication number
US3736100A
US3736100A US00146388A US3736100DA US3736100A US 3736100 A US3736100 A US 3736100A US 00146388 A US00146388 A US 00146388A US 3736100D A US3736100D A US 3736100DA US 3736100 A US3736100 A US 3736100A
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US
United States
Prior art keywords
gel
recessed portion
frame
serum
wire
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US00146388A
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English (en)
Inventor
S D Rains
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Milton Roy LLC
Original Assignee
Bausch and Lomb Inc
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Publication date
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Application granted granted Critical
Publication of US3736100A publication Critical patent/US3736100A/en
Assigned to MILTON ROY COMPANY, A CORP. OF PA. reassignment MILTON ROY COMPANY, A CORP. OF PA. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: BAUSCH & LOMB INCORPORATED
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • This invention relates to an apparatus for carrying gel for immunoelectrophoresis analysis of serums and antiserums and more particularly to a self-contained unit for supporting the gel including apparatus for individually introducing serum and anti-serum.
  • This invention provides an apparatus for expeditiously and inexpensively carrying out the process of immunm electrophoresis with dependable accurate results.
  • patent application entitled Method of and Apparatus for Depositing a Fluid in a Gel for Rains et al. there is described, in detail, a method for introducing fluids into a gel for analyzing chemical constituents.
  • R. I. Wieme in Agar Gel Electrophoresis published by Elsevier Publishing Company in 1965, describes, in detail, the particular processes of electrophoresis as well as immunoelectrophoresis.
  • the embodiments disclosed therein generally show a wire-like element which extends across the frame for introducing a fluid into a gel contained within the frame.
  • FIG. 4 is a cross-sectional view of another embodiment of the apparatus according to the principles of the present invention.
  • FIG. 1 there is disclosed a gel frame 10 having a recessed area 12 defined by parallel lateral walls 14 and 16 and transversely disposed walls 18 and 20 for carrying any gel suitable for use in the process of electrophoresis.
  • a plurality of protrusions 22, 22 and 2 Extending upward from the bottom portion of the recessed area 12 are a plurality of protrusions 22, 22 and 2 of similar construction.
  • the protrusions 22, 22' and 22" are ideally formed as an integral part of the gel frame 10 and are generally rectangular in shape.
  • Disposed at opposite sides of each of the protrusions 22, 22 and 22" are respective apertures 24 and 26-, 24 and 26 and 24 and 26".
  • the series of apertures 24, 26, 24', 26, 24" and 26" extend through the gel frame 10 from the recessed area '12 to the underside 28 of the gel frame 10.
  • inverted U-shaped' wire-like elements 30, 30' and 30" extend respectively through the series of apertures 24, 26, 24, 26 and 24", 26" to bridge the respective series of protrusions 22, 22 and 22!
  • an anti-serum of known composition to conduct particularly desired tests is disposed in the respective reservoirs 36 and 36' and the wire-like elements 38 and 38' are extracted.
  • the anti-serum fills the void left by the respective wire-like elements as described in the hereinbefore mentioned Rains et al. patent application entitled Method of and Apparatus for Depositing a Fluid in a Gel.
  • FIG. 4 A further embodiment of the present invention is disclosed in FIG. 4. Specifically, a gel frame 42 is shown having a recessed portion 44 therein. A plurality of passageways 46, 48 and 50 are located in the gel frame 42 so as to communicate the lower surface 52 of the gel frame with the bottom 54 of the recess 44. A plurality of wirelike elements '56, 8 and 60 are positioned in the passageways 46, 48 and 50, respectively, so that they do not protrude above the upper surface 62 of the gel frame 42. A gel 64 is then introduced into the recessed portion 44 of the gel frame 42 and allowed to set. Once the gel 64 has set a portion of the residual gel that remains in the conical portions of the passageways 46, 48 and 50, respectively, may be removed therefrom.
  • the removal of the small amount of residual gel is necessary to allow a fluid sample to be deposited in the conical shaped sections of the passageways 46, 48 and 50.
  • the fluid sample may be deposited by any known acceptable manner such as, for instance, was disclosed in the concurrently-filed and copending application Method of and Apparatus for De positing a Fluid in a Gel for inventors Stephen D. Rains and Leon L. Wheeless, Jr. After the fluid sample has been deposited in the passageways the wire-like elements may be withdrawn therefrom. The fluid, as previously described, is caused to flow into the voids left by the extraction of the wire-like elements.
  • the hereinbefore mentioned patent application entitled Method of and Apparatus for Depositing a Fluid in a Gel specifically describes this phenomenon.
  • the means for introducing the fluid into the gel has been called a wire-like element, although anything that resembles a wire, such as a strand of hair, a nylon or plastic filament, or even a string, would work equally as well.
  • the wire-like element may be either rigid or flexible and it is not necessarily limited to a cross-section of circular shape.
  • a strip of material such as paper or plastic, for example, can also serve as the wire-like element, as well as can probes of triangular, square or any of the cross-sectional shape.
  • An apparatus for use in the process of immunoelectrophoresis where gel is useable as a support medium comprising:
  • a frame defining a recessed portion therein for carrying gel, said recessed portion having at least one aperture communicating the recessed portion with an outer surface of the frame;
  • first filament extending through the aperture to define a first path for fluid through the aperture and gel carried within the recessed portion to facilitate the introduction of chemical constituents into gel carried within the recessed portion;
  • said second filament disposed within and traversing at least part of the length of the recessed portion to define a second separate path for fluid to facilitate the introduction of additional chemical constituents into gel carried within the recessed portion.
  • An apparatus for use in the process of immunoelectrophoresis where a gel is useable as a support medium comprising:
  • a frame defining a recessed portion therein to carry gel, said recessed portion having a plurality of apertures communicating the recessed portion with an outer surface of the frame;
  • At least one U-shaped element said element extending through two apertures to define a first continuous path for fluid through the two apertures and gel carried within the recessed portion;
  • At least one support member for said U-shaped element said support member disposed between said U-shaped element and the recessed portion to provide support for said U-shaped element; and I at least one elongated straight element positioned adjacent said U-shaped element and within the recessed portion to define a second continuous path for fluid within the gel separate from the first continuous path.
  • An apparatus for use in the process of immunoelectrophoresis where a gel is useable as a support medium comprising:
  • a frame defining a recessed portion therein for carrying gel, said recessed portion having at least one aperture communicating the recessed portion with an outer surface of the frame;
  • At least one second substantially straight element disposed within and traversing at least part of the length of the recessed portion to define a second separate path for fluid to facilitate the introduction of additional chemical constituents into gel carried within the recessed portion at the second path.
  • An apparatus for use in the process of immunoelectrophoresis where gel is carried as a support medium for analysis comprises:
  • a frame defining a recessed portion therein for carrying a gel, said recessed portion having at least one aperture communicating the recessed portion with an outer surface of the frame; a gel carried within the' recessed portion of the frame; at least one first filament, said first filament disposed in the gel and extending through the aperture to define a first path for fluid through the aperture and gel carried within the recessed portion to facilitate the introduction of chemical constituents into the gel carried within the recessed portion; and at least one second filament, said second filament disposed within the gel and traversing at least part of the length of the recessed portion to define a second separate path for fluid to facilitate the introduction of additional chemical constituents into the gel carried within the recessed portion.
  • the second filament is elongated and substantially straight.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrostatic Separation (AREA)
  • Sampling And Sample Adjustment (AREA)
US00146388A 1971-05-24 1971-05-24 Immunoelectrophoresis gel tray Expired - Lifetime US3736100A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14626271A 1971-05-24 1971-05-24
US14638871A 1971-05-24 1971-05-24
US14638771A 1971-05-24 1971-05-24

Publications (1)

Publication Number Publication Date
US3736100A true US3736100A (en) 1973-05-29

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Family Applications (3)

Application Number Title Priority Date Filing Date
US00146388A Expired - Lifetime US3736100A (en) 1971-05-24 1971-05-24 Immunoelectrophoresis gel tray
US00146387A Expired - Lifetime US3751357A (en) 1971-05-24 1971-05-24 Electrophoresis system and gel frame
US00146262A Expired - Lifetime US3762877A (en) 1971-05-24 1971-05-24 Method of and apparatus for depositing a fluid in a gel

Family Applications After (2)

Application Number Title Priority Date Filing Date
US00146387A Expired - Lifetime US3751357A (en) 1971-05-24 1971-05-24 Electrophoresis system and gel frame
US00146262A Expired - Lifetime US3762877A (en) 1971-05-24 1971-05-24 Method of and apparatus for depositing a fluid in a gel

Country Status (3)

Country Link
US (3) US3736100A (show.php)
DE (3) DE2224985A1 (show.php)
FR (1) FR2138933B1 (show.php)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844918A (en) * 1970-02-09 1974-10-29 Bioware Inc Stabilizing media template
US3947250A (en) * 1974-06-21 1976-03-30 Baxter Laboratories, Inc. Method of immunodiffusion
US3960489A (en) * 1974-04-01 1976-06-01 General Electric Company Method and apparatus for determination of concentration of immunologically reactive biological particles
US3960488A (en) * 1974-04-01 1976-06-01 General Electric Company Method and apparatus for quantitative surface inhibition test
US3988230A (en) * 1973-12-31 1976-10-26 Medac Gesellschaft Fur Klinische Spezial Praparate Mbh Chamber and process for crossed immunoelectro-phoresis
US4012198A (en) * 1972-07-25 1977-03-15 Burroughs Wellcome Co. Immunodiffusion device
US4094759A (en) * 1975-01-03 1978-06-13 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for simultaneous quantitative analysis of several constituents in a sample
US4322274A (en) * 1980-08-28 1982-03-30 Wilson Gregory B Method of diagnosing cystic fibrosis patients and asymptomatic carrier of the cystic fibrosis gene
US4329213A (en) * 1977-07-14 1982-05-11 Elwing Hans B Gel diffusion immunoassay including α-feto protein determination
US5443704A (en) * 1991-12-31 1995-08-22 Fmc Corporation Electrophoresis gel container assemblies
US20050011762A1 (en) * 2003-06-26 2005-01-20 Provonchee Richard B. Bottom access electrophoresis tray and method of use

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3865712A (en) * 1972-04-13 1975-02-11 Lkb Produkter Ab Electrode arrangement for an electrophoretical separation performed in a gel plate
US3990849A (en) * 1975-02-07 1976-11-09 Technicon Instruments Corporation Separation of cells from liquid components of blood
US3984533A (en) * 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
GB1542835A (show.php) * 1976-11-24 1979-03-28
JPS53144399A (en) * 1977-05-21 1978-12-15 Olympus Optical Co Ltd Forming device of specimen coating position marks
US4088561A (en) * 1977-06-27 1978-05-09 The United States Of America As Represented By The United States Department Of Energy Apparatus for electrophoresis separation
FR2424535A1 (fr) * 1978-04-24 1979-11-23 Aladjem Frederick Procede et appareil de mesure quantitative de la concentration de proteines
US4234400A (en) * 1979-01-26 1980-11-18 The Regents Of The University Of California Horizontal slab gel electrophoresis
US4588491A (en) * 1984-02-27 1986-05-13 International Biotechnologies, Inc. Horizontal gel electrophoresis device
US4618408A (en) * 1984-11-29 1986-10-21 Lab Stuff, Inc. Casting apparatus for electrophoretic gel tray
USD290510S (en) 1984-11-29 1987-06-23 Lab Stuff, Inc. Casting tray for electrophoretic gel
US4784738A (en) * 1985-12-12 1988-11-15 Bio-Rad Laboratories, Inc. Apparatus and method for gel casting
US4911816A (en) * 1986-02-04 1990-03-27 Oncor, Inc. Process for conducting electrophoresis and transfer
IL81145A (en) * 1987-01-02 1990-08-31 Univ Ramot Device for transfer of micro-organism sample to culture medium
US4810348A (en) * 1987-03-16 1989-03-07 Helena Laboratories Corporation Automatic electrophoresis apparatus and method
US4954237A (en) * 1987-03-16 1990-09-04 Helena Laboratories Automatic electrophoresis apparatus
US4892639A (en) * 1987-07-17 1990-01-09 Helena Laboratories Corporation Electrophoresis plate and method of making same
US4889610A (en) * 1988-07-15 1989-12-26 Life Technologies, Inc. Pop up electrophoresis apparatus and method
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US4975173A (en) * 1989-02-22 1990-12-04 Helena Laboratories Corporation Electrophoresis plate and method of making same
US5074981A (en) * 1989-04-26 1991-12-24 The University Of Tennessee Research Corporation High speed gel electrophoresis
US5228971A (en) * 1990-12-20 1993-07-20 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
US5242568A (en) * 1992-01-14 1993-09-07 Fotodyne Incorporated Electrophoresis apparatus
JP4554365B2 (ja) * 2002-09-11 2010-09-29 テンプル・ユニバーシティ−オブ・ザ・コモンウェルス・システム・オブ・ハイアー・エデュケイション 高処理能力電気泳動分離のための自動化システム

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844918A (en) * 1970-02-09 1974-10-29 Bioware Inc Stabilizing media template
US4012198A (en) * 1972-07-25 1977-03-15 Burroughs Wellcome Co. Immunodiffusion device
US3988230A (en) * 1973-12-31 1976-10-26 Medac Gesellschaft Fur Klinische Spezial Praparate Mbh Chamber and process for crossed immunoelectro-phoresis
US3960489A (en) * 1974-04-01 1976-06-01 General Electric Company Method and apparatus for determination of concentration of immunologically reactive biological particles
US3960488A (en) * 1974-04-01 1976-06-01 General Electric Company Method and apparatus for quantitative surface inhibition test
US3947250A (en) * 1974-06-21 1976-03-30 Baxter Laboratories, Inc. Method of immunodiffusion
US4094759A (en) * 1975-01-03 1978-06-13 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for simultaneous quantitative analysis of several constituents in a sample
US4329213A (en) * 1977-07-14 1982-05-11 Elwing Hans B Gel diffusion immunoassay including α-feto protein determination
US4322274A (en) * 1980-08-28 1982-03-30 Wilson Gregory B Method of diagnosing cystic fibrosis patients and asymptomatic carrier of the cystic fibrosis gene
US5443704A (en) * 1991-12-31 1995-08-22 Fmc Corporation Electrophoresis gel container assemblies
US20050011762A1 (en) * 2003-06-26 2005-01-20 Provonchee Richard B. Bottom access electrophoresis tray and method of use
WO2005001428A3 (en) * 2003-06-26 2006-03-16 Cbm Intellectual Properties In Bottom access electrophoresis tray and method of use

Also Published As

Publication number Publication date
FR2138933B1 (show.php) 1973-07-13
DE2224988A1 (de) 1972-11-30
DE2224987A1 (de) 1972-11-30
DE2224985A1 (de) 1972-11-30
US3751357A (en) 1973-08-07
US3762877A (en) 1973-10-02
FR2138933A1 (show.php) 1973-01-05

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Legal Events

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AS Assignment

Owner name: MILTON ROY COMPANY, ONE PLAZA PLACE, ST. PETERSBUR

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:BAUSCH & LOMB INCORPORATED;REEL/FRAME:004454/0288

Effective date: 19850415