US3713986A - Indicator for the determination of reduced pyridine coenzymes - Google Patents
Indicator for the determination of reduced pyridine coenzymes Download PDFInfo
- Publication number
- US3713986A US3713986A US00090467A US3713986DA US3713986A US 3713986 A US3713986 A US 3713986A US 00090467 A US00090467 A US 00090467A US 3713986D A US3713986D A US 3713986DA US 3713986 A US3713986 A US 3713986A
- Authority
- US
- United States
- Prior art keywords
- determination
- indicator
- nadh
- reduced
- coenzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 title abstract description 36
- 239000005515 coenzyme Substances 0.000 title abstract description 19
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 title abstract description 18
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract description 20
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 abstract description 11
- 239000001301 oxygen Substances 0.000 abstract description 11
- 239000001257 hydrogen Substances 0.000 abstract description 7
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 7
- -1 BENZOPHENOTHIAZINE COMPOUND Chemical class 0.000 abstract description 6
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical class [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052717 sulfur Inorganic materials 0.000 abstract description 4
- 239000011593 sulfur Substances 0.000 abstract description 4
- YOSZEPWSVKKQOV-UHFFFAOYSA-N 12h-benzo[a]phenoxazine Chemical compound C1=CC=CC2=C3NC4=CC=CC=C4OC3=CC=C21 YOSZEPWSVKKQOV-UHFFFAOYSA-N 0.000 abstract description 2
- XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical group [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 description 24
- 239000000243 solution Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 13
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 11
- 125000003831 tetrazolyl group Chemical group 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 6
- 108091006149 Electron carriers Proteins 0.000 description 5
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 5
- 229950006238 nadide Drugs 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 125000004432 carbon atom Chemical group C* 0.000 description 3
- 125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- JORABGDXCIBAFL-UHFFFAOYSA-M iodonitrotetrazolium chloride Chemical compound [Cl-].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C=CC=CC=2)=N1 JORABGDXCIBAFL-UHFFFAOYSA-M 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical group [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 1
- DMBARTDJBIKHNL-UHFFFAOYSA-N NC=1C=2C=CC=CC2CC2=C3C(C=CC12)=CC=NO3 Chemical compound NC=1C=2C=CC=CC2CC2=C3C(C=CC12)=CC=NO3 DMBARTDJBIKHNL-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000007999 glycylglycine buffer Substances 0.000 description 1
- 239000005337 ground glass Substances 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- UOVTXCOUQSQRBQ-UHFFFAOYSA-N n,n-dimethyl-12h-naphtho[2,3-h][1,2]benzoxazin-7-amine Chemical compound C1C2=CC=CC=C2C(N(C)C)=C2C1=C1ON=CC=C1C=C2 UOVTXCOUQSQRBQ-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 125000001484 phenothiazinyl group Chemical group C1(=CC=CC=2SC3=CC=CC=C3NC12)* 0.000 description 1
- 125000001644 phenoxazinyl group Chemical group C1(=CC=CC=2OC3=CC=CC=C3NC12)* 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 238000010405 reoxidation reaction Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/141111—Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]
Definitions
- R and R are hydrogen or lower alkyl of from 1 to 6 carbon atoms; X is sulfur or oxygen; and Y is hydrogen or hydroxyl.
- the present invention is concerned with a colorimetric method for the determination of reduced pyridine coenzymes, such as nicotinamide adenine dinucleotide (NADH) or nicotinamide-adenine-dinucleotide phosphate (NADPH).
- NADH nicotinamide adenine dinucleotide
- NADPH nicotinamide-adenine-dinucleotide phosphate
- the reduced pyridine coenzymes are well-known indicators in analytical biochemistry. With their use, it is possible to determine a wide variety of metabolic products in the animal and vegetable kingdoms. Furthermore, in recent times, the determinations of enzymes which are possible with the use of these pyridine coenzymes have achieved an enormous importance in the diagnosis of damage to or disfunction of organs in human and veterinary medicine.
- R and R which may be the same or different, are hydrogen atoms or lower alkyl radicals containing up to 6 carbon atoms; X is sulfur or oxygen and Y is hydrogen or hydroxyl; and used as indicators for the determination of the reduced pyridine coenzymes.
- the compounds to be used according to the present invention are themselves colored and thus permit the direct determination of the reduced pyridine coenzymes since they are decolorised by them. Thus, it is possible simply to titrate the reduced pyridine coenzymes with solutions of the indicators to be used according to the present invention. In this way, a determination of the reduced pyridine coenzymes is possible with only a small expenditure for apparatus.
- the compounds to be used according to the present invention can also act as electron carriers to tetrazolium salts with the formation of coloured formazanes and can, therefore be used in the same manner as the two above-mentioned known electron carriers.
- camparison with these two known compounds they have the advantage of a better stability and, in particular, they can also be stored at ambient temperature for long periods of time.
- the compounds to be used according to the present invention are characterized by the presence of a benzene ring in the 2,3- or 2,1-position of the phenoxazine or phenothiazine ring system.
- This fused benzene ring can be substituted by a hydroxyl group, which is preferably in the 2'- or 3'- position.
- Preferred compounds to be used according to the present invention are 7-dimethylamino-1,Z-benzophenoxazine (Meldolblue), 7-dimethylamino-2-hydroxy-1,2-benzophenoxazine, 7-dimethylamino-3-hydroxy-1,2-benzophenoxazine, 7-amino-1,2 benzophenoxazine,
- the compounds to be used according to the present invention are known.
- the amounts used depend upon whether there is to be carried out a direct determination by decolorisation or whether the compound is to be used as a carrier to a tetrazolium salt.
- a direct determination by decolorisation or whether the compound is to be used as a carrier to a tetrazolium salt In the first case, it is a matter of a stoichiometric reaction and there must, therefore, be used stoichiometric amounts, referred to the reduced pyridine coenzyme. In the latter case, it is a question of a catalytic action, in which the compounds act as electron carriers. In this case, it is suflicient to use very small amounts of the compounds.
- Some of the compounds to be used according to the present invention are water-soluble, whereas others are Water-insoluble.
- the water-insoluble compounds are used dissolved in aqueous or pure alcohols, preferably in isopropanol.
- tetrazolium salts which have been previously employed for this purpose.
- Those which have proved to be particularly useful are 3-(4,5-dimethyl-thiazo1yl-2)-2,5-diphenyl-tetrazolium bromide and iodonitrotetrazolium chloride.
- reaction vessel there is used a closed vessel which is flushed with an inert gas.
- the compounds to be used according to the present invention can, as a result of their stability, also be employed, for example, when applied to carrier materials, such as paper strips, gel layers and the like, as indicators for the determination of pyridine coenzyme activities.
- EXAMPLE 2 The compounds set out in the following table were used for the determination of the activity of lactate dehydrogenase, phenazine-methosulfate (PMS) being used as comparison compound.
- the test was commenced with 0.02 ml. purified lactate dehydrogenase solution (0.068 IU).
- Method for the determination of reduced pyridine coenzymes which comprises contacting a test mixture with an indicator comprising a compound of the general formula:
- R and R are hydrogen or lower alkyl of tfrom 1 to 6 carbon atoms; X is sulfur or oxygen; and Y is hydrogen or hydroxyl.
- said indicator comprises, additionally, a tetrazolium salt.
- tetrazolium salt is 3 (4,5 dimethyl thiazolyl 2)-2,5- diphenyl-tetrazolium bromide or iodonitrotetrazolium chloride.
- composition for the colorimetric determination of reduced pyridine coenzymes which composition comprises as an indicator a compound as recited in claim 1 and, in addition to said compound, a tetrazolium salt.
- composition as claimed in claim 17 wherein the tetrazolium salt is 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide or iodonitrotetrazolium chloride.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Plural Heterocyclic Compounds (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1959410A DE1959410C3 (de) | 1969-11-26 | 1969-11-26 | Indikator zur Bestimmung der reduzierten Pyridincöenzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US3713986A true US3713986A (en) | 1973-01-30 |
Family
ID=5752161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US00090467A Expired - Lifetime US3713986A (en) | 1969-11-26 | 1970-11-17 | Indicator for the determination of reduced pyridine coenzymes |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US3713986A (enrdf_load_stackoverflow) |
| BR (1) | BR7024172D0 (enrdf_load_stackoverflow) |
| DE (1) | DE1959410C3 (enrdf_load_stackoverflow) |
| FR (1) | FR2072394A5 (enrdf_load_stackoverflow) |
| GB (1) | GB1299845A (enrdf_load_stackoverflow) |
| IL (1) | IL35487A0 (enrdf_load_stackoverflow) |
| NL (1) | NL7016811A (enrdf_load_stackoverflow) |
| SE (1) | SE364368B (enrdf_load_stackoverflow) |
| ZA (1) | ZA707604B (enrdf_load_stackoverflow) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3881992A (en) * | 1973-07-02 | 1975-05-06 | Wilson Ralston | Optical rate measurement method |
| US4003795A (en) * | 1974-09-12 | 1977-01-18 | Kommanditgesellschaft Schwarzhaupt | Process for the determination of at least one of the isoenzymes of lactatendehydrogenase |
| US4038146A (en) * | 1974-06-07 | 1977-07-26 | Latron Laboratories, Inc. | Method of determination of serum triglycerides and reagents |
| US4142938A (en) * | 1974-03-20 | 1979-03-06 | The Dow Chemical Company | Determination of triglycerides and glycerol |
| US4851353A (en) * | 1980-04-14 | 1989-07-25 | Kyowa Hakko Kogyo Co., Ltd. | Method and test composition for determination of lipid peroxide |
| US5426032A (en) * | 1986-08-13 | 1995-06-20 | Lifescan, Inc. | No-wipe whole blood glucose test strip |
| US6092003A (en) * | 1998-01-26 | 2000-07-18 | Honeywell-Measurex Corporation | Paper stock shear and formation control |
| US6458326B1 (en) | 1999-11-24 | 2002-10-01 | Home Diagnostics, Inc. | Protective test strip platform |
| US6525330B2 (en) | 2001-02-28 | 2003-02-25 | Home Diagnostics, Inc. | Method of strip insertion detection |
| US6541266B2 (en) | 2001-02-28 | 2003-04-01 | Home Diagnostics, Inc. | Method for determining concentration of an analyte in a test strip |
| US6562625B2 (en) | 2001-02-28 | 2003-05-13 | Home Diagnostics, Inc. | Distinguishing test types through spectral analysis |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL7500951A (nl) * | 1975-01-28 | 1976-07-30 | Akzo Nv | Fluorimetrisch aantonen en bepalen van een gereduceerd co-enzym of derivaat in een waterig systeem. |
| US4141688A (en) * | 1977-08-11 | 1979-02-27 | Miles Laboratories, Inc. | Composition, device and method for determining reducing agents |
| DE2834705B1 (de) * | 1978-08-08 | 1980-01-24 | Boehringer Mannheim Gmbh | Verfahren und Reagenz zur Durchfuehrung enzymatischer Bestimmungen |
| JPS6033479B2 (ja) * | 1980-07-30 | 1985-08-02 | 協和醗酵工業株式会社 | 過酸化水素の定量方法 |
| DE3046741A1 (de) * | 1980-12-11 | 1982-07-15 | Boehringer Mannheim Gmbh, 6800 Mannheim | Nachweis von nad(p)h oder salicylat |
| AU8043982A (en) * | 1981-02-12 | 1982-08-19 | Self, C.H. | Method of detecting phosphatase by contacting with nicotinamide coenzyme |
-
1969
- 1969-11-26 DE DE1959410A patent/DE1959410C3/de not_active Expired
-
1970
- 1970-10-20 IL IL35487A patent/IL35487A0/xx unknown
- 1970-11-10 ZA ZA707604A patent/ZA707604B/xx unknown
- 1970-11-17 US US00090467A patent/US3713986A/en not_active Expired - Lifetime
- 1970-11-17 NL NL7016811A patent/NL7016811A/xx unknown
- 1970-11-24 GB GB55847/70A patent/GB1299845A/en not_active Expired
- 1970-11-24 SE SE15893/70A patent/SE364368B/xx unknown
- 1970-11-25 FR FR7042373A patent/FR2072394A5/fr not_active Expired
- 1970-11-26 BR BR224172/70A patent/BR7024172D0/pt unknown
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3881992A (en) * | 1973-07-02 | 1975-05-06 | Wilson Ralston | Optical rate measurement method |
| US4142938A (en) * | 1974-03-20 | 1979-03-06 | The Dow Chemical Company | Determination of triglycerides and glycerol |
| US4038146A (en) * | 1974-06-07 | 1977-07-26 | Latron Laboratories, Inc. | Method of determination of serum triglycerides and reagents |
| US4003795A (en) * | 1974-09-12 | 1977-01-18 | Kommanditgesellschaft Schwarzhaupt | Process for the determination of at least one of the isoenzymes of lactatendehydrogenase |
| US4851353A (en) * | 1980-04-14 | 1989-07-25 | Kyowa Hakko Kogyo Co., Ltd. | Method and test composition for determination of lipid peroxide |
| US20030054427A1 (en) * | 1986-08-13 | 2003-03-20 | Roger Phillips | Minimum procedure system for the determination of analytes |
| US6821483B2 (en) | 1986-08-13 | 2004-11-23 | Lifescan, Inc. | Reagents test strip with alignment notch |
| US5843692A (en) * | 1986-08-13 | 1998-12-01 | Lifescan, Inc. | Automatic initiation of a time interval for measuring glucose concentration in a sample of whole blood |
| US6268162B1 (en) | 1986-08-13 | 2001-07-31 | Lifescan, Inc. | Reflectance measurement of analyte concentration with automatic initiation of timing |
| US5563042A (en) * | 1986-08-13 | 1996-10-08 | Lifescan, Inc. | Whole blood glucose test strip |
| US6887426B2 (en) | 1986-08-13 | 2005-05-03 | Roger Phillips | Reagents test strip adapted for receiving an unmeasured sample while in use in an apparatus |
| US5426032A (en) * | 1986-08-13 | 1995-06-20 | Lifescan, Inc. | No-wipe whole blood glucose test strip |
| US6881550B2 (en) | 1986-08-13 | 2005-04-19 | Roger Phillips | Method for the determination of glucose employing an apparatus emplaced matrix |
| US20030073153A1 (en) * | 1986-08-13 | 2003-04-17 | Roger Phillips | Minimum procedure system for the determination of analytes |
| US20030073151A1 (en) * | 1986-08-13 | 2003-04-17 | Roger Phillips | Minimum procedure system |
| US20030073152A1 (en) * | 1986-08-13 | 2003-04-17 | Roger Phillips | Minimum procedure system for the determination of analytes |
| US6858401B2 (en) | 1986-08-13 | 2005-02-22 | Lifescan, Inc. | Minimum procedure system for the determination of analytes |
| US6092003A (en) * | 1998-01-26 | 2000-07-18 | Honeywell-Measurex Corporation | Paper stock shear and formation control |
| US6458326B1 (en) | 1999-11-24 | 2002-10-01 | Home Diagnostics, Inc. | Protective test strip platform |
| US6979571B2 (en) | 1999-11-24 | 2005-12-27 | Home Diagnostics, Inc. | Method of using a protective test strip platform for optical meter apparatus |
| US20030138356A1 (en) * | 2001-02-28 | 2003-07-24 | Home Diagnostics, Inc. | Distinguishing test types through spectral analysis |
| US6562625B2 (en) | 2001-02-28 | 2003-05-13 | Home Diagnostics, Inc. | Distinguishing test types through spectral analysis |
| US6541266B2 (en) | 2001-02-28 | 2003-04-01 | Home Diagnostics, Inc. | Method for determining concentration of an analyte in a test strip |
| US6525330B2 (en) | 2001-02-28 | 2003-02-25 | Home Diagnostics, Inc. | Method of strip insertion detection |
| US7390665B2 (en) | 2001-02-28 | 2008-06-24 | Gilmour Steven B | Distinguishing test types through spectral analysis |
Also Published As
| Publication number | Publication date |
|---|---|
| DE1959410C3 (de) | 1974-02-28 |
| ZA707604B (en) | 1971-08-25 |
| DE1959410B2 (de) | 1973-08-02 |
| DE1959410A1 (de) | 1971-06-16 |
| IL35487A0 (en) | 1970-12-24 |
| BR7024172D0 (pt) | 1973-04-26 |
| GB1299845A (en) | 1972-12-13 |
| FR2072394A5 (enrdf_load_stackoverflow) | 1971-09-24 |
| NL7016811A (enrdf_load_stackoverflow) | 1971-05-28 |
| SE364368B (enrdf_load_stackoverflow) | 1974-02-18 |
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