Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
  • Y10T436/2525—Stabilizing or preserving

Definitions

• the present invention relates to a process for the preparation, preservation and transportation of blood and serum samples for use thereof in the determination of chemical and biochemical components contained therein. More particularly the invention relates to a process for the preparation, preservation and transportation of blood and serum samples for use in the determination of chemical and biochemical components contained therein such as sugars, i.e., glucose and galactose, lipids, such as triglycerides and cholesterol, etc.
• the process according to the present invention permits the determination in blood not only of sugars, such as glucose and galactose, but also of lipids, such as triglycerides and cholesterol. Since all of the operations which are necessary for obtaining serum from whole blood are eliminated, the amount of blood necessary for the determinations can be reduced from 0.5 ml. to 0.05 ml. This is particularly important for large scale investigations as the small amount of blood needed can be drawn as capillary blood, vena-puncture no longer being necessary.
• a further advantage of the process according to the invention is that several blood samples can be taken from the same small laboratory animal in serial tests without causing any harm to the animal. This, of course, results in that the degree of accuracy of the results obtained is considerably increased.
• the prepared and dried samples obtained by the process according to the present invention can simply be transported and stored in an envelope and, yet, can be used for the carrying out of quantitative determinations with a great degree of accuracy, even following the lapse of considerable time after the taking of the blood samples.
• ion exchange paper As absorbent carriers having ion exchange properties, it is preferred to use ion exchange papers.
• ion exchange paper there can be used, depending upon the nature of the blood or serum components which are to be determined, not only cation exchangers but also anion exchangers.
• Glucose and galactose are preferably stabilized, i.e., preserved in the weakly acidic range, too low pH values having to be avoided in connection with carrying out glucose determination since otherwise glucose is split off from the cellulose forming part of the paper which would then inaccurately result in glucose values which are too high.
• anion exchange papers Since triglycerides are, in the course of enzymatic investigations, subjected to alkaline saponification, anion exchange papers have proved to be particularly well suited for the preparation and stabilization, i.e., preservation of samples intended for this purpose. Furthermore, samples intended for cholesterol determinations are also best stabilized on weakly basic ion exchange papers.
• absorbent carriers such as for instance compressed cellulose, hydrophilic synthetic resins, textile fabrics, wicks or layers of organic or inorganic powders or granulates having ion exchange properties fixed to suitable substrates by means of adhesives, such as by the addition of glue, it being immaterial whether the ion exchange properties depend upon the molecular structure of the absorbent carrier or are imparted to the carrier by a subsequent impregnation with suitable materials.
• galactose takes place, after elution of the absorbent carrier, utilizing enzyme galactose dehydrogenase, and using Warburg's optical test at 366 nm.
• the determination of cholesterol is carried out, after elution of the absorbent carrier with a suitable organic solvent, preferably with a mixture of chloroform and methanol (2:1), using for the determination the analytical method described by Watson (D. Clin. Chim. Acta, 5, 637/ I960).
• EXAMPLE 1 Serial determination of the blood glucose content in rabbits (with additional glucose loading).
• Whatman cation exchanger paper WA 2 (AMBERLITE IR-C 50) was cut up into squares having 3 cm. edges.
• the prepared pieces of paper were eluted by the method described in Example I, and thereafter the eluate was also analyzed using the Warburgs optical test.
• the mentioned ion-exchange papers named Whatman Amberlitev JRC 50 and JRA 400 are cellulose filter papers containing poly-methacrylic acid resins and polystyrene resins with quarternary ammonio groups. They are manufactured by Whatman and Balstone Ltd. Maidstone, England.
• Process for the preparation, preservation and transportation of blood and serum samples for use in the analytical determination of chemical and biochemical components contained therein which comprises applying onto an absorbent carrier having ion exchange properties a sample of serum or blood and drying the absorbent carrier and sample.
• said absorbent carrier is a member selected from the group consisting of paper, compressed cellulose, textile fabrics and hydrophilic synthetic resins.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Chemical & Material Sciences (AREA)
• Molecular Biology (AREA)
• Hematology (AREA)
• Immunology (AREA)
• Biomedical Technology (AREA)
• Urology & Nephrology (AREA)
• Biochemistry (AREA)
• Physics & Mathematics (AREA)
• Analytical Chemistry (AREA)
• Biotechnology (AREA)
• General Health & Medical Sciences (AREA)
• Microbiology (AREA)
• Cell Biology (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• General Physics & Mathematics (AREA)
• Pathology (AREA)
• Organic Chemistry (AREA)
• Wood Science & Technology (AREA)
• Biophysics (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Zoology (AREA)
• Emergency Medicine (AREA)
• Diabetes (AREA)
• Bioinformatics & Cheminformatics (AREA)
• General Engineering & Computer Science (AREA)
• Genetics & Genomics (AREA)
• Endocrinology (AREA)
• Investigating Or Analysing Biological Materials (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Applications Claiming Priority (2)

Publications (1)

Family

ID=25755644

Family Applications (1)

Country Status (6)

Cited By (10)

Citations (2)

• 1969
  • 1969-06-11 US US832432A patent/US3645692A/en not_active Expired - Lifetime
  • 1969-06-11 CA CA054099A patent/CA928198A/en not_active Expired
  • 1969-07-11 GB GB34990/69A patent/GB1235577A/en not_active Expired
  • 1969-07-14 BR BR210695/69A patent/BR6910695D0/pt unknown
  • 1969-07-14 SE SE09939/69A patent/SE352963B/xx unknown
  • 1969-07-15 ES ES369558A patent/ES369558A1/es not_active Expired

Patent Citations (2)

Non-Patent Citations (1)

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