US3607094A - Apparatus for pipetting and adding a liquid - Google Patents

Apparatus for pipetting and adding a liquid Download PDF

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Publication number
US3607094A
US3607094A US793990*A US3607094DA US3607094A US 3607094 A US3607094 A US 3607094A US 3607094D A US3607094D A US 3607094DA US 3607094 A US3607094 A US 3607094A
Authority
US
United States
Prior art keywords
pump
piston
cylinder
actuating
liquid sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US793990*A
Other languages
English (en)
Inventor
Palle-Finn Beer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Autokemi AB
Original Assignee
Autokemi AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Autokemi AB filed Critical Autokemi AB
Application granted granted Critical
Publication of US3607094A publication Critical patent/US3607094A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • B01L3/0206—Burettes, i.e. for withdrawing and redistributing liquids through different conduits of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

Definitions

  • the present invention relates to an arrangement in a pump for p I; etting a liquid sample and for adding a liquid reagent.
  • the invention is especially adapted for use in micropipetting, ie when the pipetting takes place with volumes smaller than 15 microliters, but it is not restricted to this application and can be used with advantage when it is a question of pipetting very accurately determined volumes.
  • a pump comprising a pump cylinder, a pump piston insertable in said pump cylinder, and actuation means for said pump piston, a tubular cannula connected to said pump cylinder for drawing up a liquid sample, a conduit for supplying liquid reagent to said pump cylinder, said conduit having provided therein a valve means adapted to be actuated to its open position when the pump piston reaches a position corresponding to an emptying of the pump cylinder.
  • the valve means provided in the conduit preferably comprises a valve element held in closed position by spring means. Opening of the valve means may be achieved by employing as the piston pump one having the form of a rod-shaped displacement piston and adapting the piston rod to lift the valve element from its closed position when the pump rod reaches the position corresponding to an emptying of the pump cylinder.
  • the actuating means for the pump piston is preferably com posed of an actuating piston slidably disposed and springloaded in a housing, the actuating piston being connected to the pump piston, and means for supplying compressed air to drive the actuating piston.
  • the compressed air acts on one side of actuating piston 10 and on the other side thereof a constant pressure is maintained through an aperture 18 provided in the wall of the cylinder.
  • the pump cylinder 8 communicates directly with the tubular cannula 6 and is also connected through a valve 19 with a conduit 20 for supplying liquid reagent from container 5.
  • This conduit also has provided in it a pump 21 for the liquid reagent and a valve means 22 which is controlled by control means 15.
  • valve member 23 of the valve 19 is held by a spring 24 in seated position and can be unseated against the action of spring 24 by the pump piston 9 pushing against valve member 23 in the position of the pump piston corresponding to a complete emptying of the pump cylinder.
  • cannula 6 With the pump piston 9 in the uppermost position with reference to the drawing, cannula 6 is lowered into test tube 3 after which valve means 14 is actuated by control means 15 to a position in which the compressed air does not act on actuating piston 10. The latter is then brought by spring 12 to the lowermost position with reference to the drawing as defined by rod 16. This causes a predetermined quantity of liquid sample to be drawn into cannula 6 corresponding to the displacement of piston 9, and during this movement of the piston valve 19 remains closed whereby the quantity of liquid sample that is drawn into cannula 6 is determined exclusively by the displacement of the piston in cylinder 8.
  • Cannula 6 is then moved along the path shown in dotted lines to test tube 4 and valve means 14 is actuated by control means 15 so as to cause compressed air again to be supplied to the pump.
  • Actuating piston 10 with pump piston 9 are then displaced against the action of spring 12 to the uppermost position and the quantity of liquid sample drawn into cannula 6 is emptied into test tube 4.
  • control means 15 actuates the other valve means 22 causing the connection between liquid reagent pump 21 and conduit 20 to be established.
  • An apparatus for pipetting a liquid sample and supplying a liquid reagent which comprises a pump cylinder, a pump piston insertable into said pump cylinder, a tubular cannula connected to said pump cylinder, actuating means for displacing said pump piston from said pump cylinder to draw liquid sample from a source and for inserting said pump piston into said pump cylinder to discharge said drawn liquid sample to a location other than said source, means for moving said tubular cannula to said other location, a conduit for supplying liquid reagent to said pump cylinder for delivery through said tubular cannula to said liquid sample location subsequent to discharge of said liquid sample, said conduit being provided with a valve means, said valve means being in closed position during said drawing and discharge of liquid sample and in open position upon discharge of said liquid sample, opening of said valve means being effected by said pump piston upon insertion through said chamber, whereby the reagent can enter said pump cylinder.
  • valve means comprises a valve element held in closed position by spring means and the pump piston has the form of a rod-shaped displacement piston for lifting said valve element from said closed position.
  • actuating means comprises an actuating piston slidably disposed and springloaded in a housing, said actuating piston being connected to said pump, and means for supplying compressed air to drive said actuating piston.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
US793990*A 1968-01-25 1969-01-27 Apparatus for pipetting and adding a liquid Expired - Lifetime US3607094A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE01020/68A SE327840B (de) 1968-01-25 1968-01-25

Publications (1)

Publication Number Publication Date
US3607094A true US3607094A (en) 1971-09-21

Family

ID=20257459

Family Applications (1)

Application Number Title Priority Date Filing Date
US793990*A Expired - Lifetime US3607094A (en) 1968-01-25 1969-01-27 Apparatus for pipetting and adding a liquid

Country Status (7)

Country Link
US (1) US3607094A (de)
JP (1) JPS4947477B1 (de)
DE (1) DE1902297B2 (de)
FR (1) FR2000695A1 (de)
GB (1) GB1256746A (de)
NL (1) NL6900596A (de)
SE (1) SE327840B (de)

Cited By (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3699348A (en) * 1970-08-14 1972-10-17 Zeiss Stiftung Radiation sensitive device for the successive investigation of several samples
US3735902A (en) * 1971-07-22 1973-05-29 Instrumentation Labor Inc Dispenser apparatus
US3739821A (en) * 1971-04-26 1973-06-19 Technicon Instr Machine-transferrable pipette
US3767364A (en) * 1971-07-30 1973-10-23 Sherwood Medical Ind Inc Reagent pipette system
US3827304A (en) * 1971-07-20 1974-08-06 Gilson W Sample handling method
DE2622691A1 (de) * 1976-05-21 1977-12-01 Technicon Instr Probenanalysegeraet
DE3015051A1 (de) * 1979-04-19 1980-10-23 Olympus Optical Co Verfahren zur abgabe von proben und reagenzien in reaktionsgefaesse
DE3033680A1 (de) * 1979-09-10 1981-03-12 Olympus Optical Co., Ltd., Tokyo Dosier- und leitungssystem fuer analysegeraete
US4318884A (en) * 1979-12-07 1982-03-09 Olympus Optical Co., Ltd. Distributing nozzle with cooperating ionic detector
US4340153A (en) * 1980-11-28 1982-07-20 Spivey David L Method and apparatus for medication dispensing
US4399711A (en) * 1980-04-18 1983-08-23 Beckman Instruments, Inc. Method and apparatus ensuring full volume pickup in an automated pipette
US4727033A (en) * 1981-12-17 1988-02-23 Olympus Optical Co., Ltd. Analyzing apparatus and method for immunological agglutination reactions
WO1990008307A1 (en) * 1988-12-29 1990-07-26 Technicon Instruments Corporation Integrated sampler for closed and open sample containers
US5158748A (en) * 1990-01-18 1992-10-27 Mochida Pharmaceutical Co., Ltd. Automated dispensing and diluting system
US5364240A (en) * 1993-10-14 1994-11-15 Spartan Tool Div. Of Pettibone Corp. Fluid pump with pulsing feature
US5795784A (en) * 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5841039A (en) * 1991-04-04 1998-11-24 Technicon Instruments Corp. Apparatus and method for integrated sampling from closed and open sample containers
US5856194A (en) * 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US6302168B1 (en) * 2000-07-07 2001-10-16 Hugo Hu Automated precision liquid metering apparatus using injectors as metering devices
US20010034067A1 (en) * 2001-01-26 2001-10-25 Dales G. Cameron Apparatus and methods for parallel processing of multiple reaction mixtures
US20020110493A1 (en) * 1998-08-13 2002-08-15 Symyx Technologies , Inc Apparatus and methods for parallel processing of multiple reaction mixtures
US6864091B1 (en) * 2000-08-31 2005-03-08 Symyx Technologies, Inc. Sampling probe
US20090110606A1 (en) * 2006-06-21 2009-04-30 Olympus Corporation Dispensing apparatus and analyzer
US20110253750A1 (en) * 2010-04-20 2011-10-20 Koganei Corporation Liquid supply device
US8372340B2 (en) 2005-10-19 2013-02-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8476078B2 (en) 2005-10-19 2013-07-02 Luminex Corporation Cassette for sample preparation
US8900877B2 (en) 2006-12-27 2014-12-02 Luminex Corporation Instrument for cassette for sample preparation
AU2014201790B2 (en) * 2005-10-19 2015-12-17 Luminex Corporation Cassette for Sample Preparation
US9248422B2 (en) 2010-02-23 2016-02-02 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
AU2016201550B2 (en) * 2005-10-19 2017-07-27 Luminex Corporation Cassette for sample preparation
US11293416B2 (en) * 2017-09-21 2022-04-05 Hartmann's, Inc. Precision fluid dispensing device

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2910946A1 (de) * 1979-03-20 1980-10-02 Siemens Ag Dosierspritze
FR2513376A1 (fr) * 1981-09-23 1983-03-25 Jean Bajard Dispositif de pipetage automatique
DE10201587A1 (de) * 2002-01-17 2003-08-14 Renk Ag Elastische Bolzenkupplung

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1896134A (en) * 1929-02-25 1933-02-07 Dolph M Clark Liquid dispensing apparatus
US2216890A (en) * 1939-12-01 1940-10-08 Dalkin & Co A Measuring and mixing valve
US3193359A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for conducting analytical procedural steps
US3421858A (en) * 1965-12-07 1969-01-14 Hewlett Packard Co Sampling apparatus
US3475130A (en) * 1965-07-08 1969-10-28 Warner Lambert Pharmaceutical Sample transfer apparatus

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1896134A (en) * 1929-02-25 1933-02-07 Dolph M Clark Liquid dispensing apparatus
US2216890A (en) * 1939-12-01 1940-10-08 Dalkin & Co A Measuring and mixing valve
US3193359A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for conducting analytical procedural steps
US3475130A (en) * 1965-07-08 1969-10-28 Warner Lambert Pharmaceutical Sample transfer apparatus
US3421858A (en) * 1965-12-07 1969-01-14 Hewlett Packard Co Sampling apparatus

Cited By (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3699348A (en) * 1970-08-14 1972-10-17 Zeiss Stiftung Radiation sensitive device for the successive investigation of several samples
US3739821A (en) * 1971-04-26 1973-06-19 Technicon Instr Machine-transferrable pipette
US3827304A (en) * 1971-07-20 1974-08-06 Gilson W Sample handling method
US3735902A (en) * 1971-07-22 1973-05-29 Instrumentation Labor Inc Dispenser apparatus
US3767364A (en) * 1971-07-30 1973-10-23 Sherwood Medical Ind Inc Reagent pipette system
DE2622691A1 (de) * 1976-05-21 1977-12-01 Technicon Instr Probenanalysegeraet
DE3015051A1 (de) * 1979-04-19 1980-10-23 Olympus Optical Co Verfahren zur abgabe von proben und reagenzien in reaktionsgefaesse
DE3033680A1 (de) * 1979-09-10 1981-03-12 Olympus Optical Co., Ltd., Tokyo Dosier- und leitungssystem fuer analysegeraete
US4318885A (en) * 1979-09-10 1982-03-09 Olympus Optical Co., Ltd. Liquid treating device for chemical analysis apparatus
US4318884A (en) * 1979-12-07 1982-03-09 Olympus Optical Co., Ltd. Distributing nozzle with cooperating ionic detector
US4399711A (en) * 1980-04-18 1983-08-23 Beckman Instruments, Inc. Method and apparatus ensuring full volume pickup in an automated pipette
US4340153A (en) * 1980-11-28 1982-07-20 Spivey David L Method and apparatus for medication dispensing
US4727033A (en) * 1981-12-17 1988-02-23 Olympus Optical Co., Ltd. Analyzing apparatus and method for immunological agglutination reactions
WO1990008307A1 (en) * 1988-12-29 1990-07-26 Technicon Instruments Corporation Integrated sampler for closed and open sample containers
US5201232A (en) * 1988-12-29 1993-04-13 Technicon Instruments Corporation Integrated sampler for closed and open sample containers
US5158748A (en) * 1990-01-18 1992-10-27 Mochida Pharmaceutical Co., Ltd. Automated dispensing and diluting system
US5841039A (en) * 1991-04-04 1998-11-24 Technicon Instruments Corp. Apparatus and method for integrated sampling from closed and open sample containers
US5364240A (en) * 1993-10-14 1994-11-15 Spartan Tool Div. Of Pettibone Corp. Fluid pump with pulsing feature
US5795784A (en) * 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) * 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US6562298B1 (en) 1996-09-19 2003-05-13 Abbott Laboratories Structure for determination of item of interest in a sample
US6913934B2 (en) 1998-08-13 2005-07-05 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US20020110493A1 (en) * 1998-08-13 2002-08-15 Symyx Technologies , Inc Apparatus and methods for parallel processing of multiple reaction mixtures
US6302168B1 (en) * 2000-07-07 2001-10-16 Hugo Hu Automated precision liquid metering apparatus using injectors as metering devices
US6864091B1 (en) * 2000-08-31 2005-03-08 Symyx Technologies, Inc. Sampling probe
US7071000B2 (en) 2000-08-31 2006-07-04 Symyx Technologies, Inc. Method for sampling reaction products
US20040241875A1 (en) * 2001-01-26 2004-12-02 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US6759014B2 (en) 2001-01-26 2004-07-06 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US20010034067A1 (en) * 2001-01-26 2001-10-25 Dales G. Cameron Apparatus and methods for parallel processing of multiple reaction mixtures
AU2014201790B2 (en) * 2005-10-19 2015-12-17 Luminex Corporation Cassette for Sample Preparation
US10646875B2 (en) 2005-10-19 2020-05-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8372340B2 (en) 2005-10-19 2013-02-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8476078B2 (en) 2005-10-19 2013-07-02 Luminex Corporation Cassette for sample preparation
US10472622B2 (en) 2005-10-19 2019-11-12 Luminex Corporation Cassette for sample preparation
US10040071B2 (en) 2005-10-19 2018-08-07 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US9017617B2 (en) 2005-10-19 2015-04-28 Luminex Corporation Cassette for sample preparation
US9074250B2 (en) 2005-10-19 2015-07-07 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US9624531B2 (en) 2005-10-19 2017-04-18 Luminex Corporation Cassette for sample preparation
US9828598B2 (en) 2005-10-19 2017-11-28 Luminex Corporation Cassette for sample preparation
AU2016201550B2 (en) * 2005-10-19 2017-07-27 Luminex Corporation Cassette for sample preparation
US9539577B2 (en) 2005-10-19 2017-01-10 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US20090110606A1 (en) * 2006-06-21 2009-04-30 Olympus Corporation Dispensing apparatus and analyzer
US9273344B2 (en) 2006-12-27 2016-03-01 Luminex Corporation Instrument for cassette for sample preparation
US9434939B2 (en) 2006-12-27 2016-09-06 Luminex Corporation Instrument for cassette for sample preparation
US9745615B2 (en) 2006-12-27 2017-08-29 Luminex Corporation Instrument for cassette for sample preparation
US9856517B2 (en) 2006-12-27 2018-01-02 Luminex Corporation Instrument for cassette for sample preparation
US8900877B2 (en) 2006-12-27 2014-12-02 Luminex Corporation Instrument for cassette for sample preparation
US10047391B2 (en) 2006-12-27 2018-08-14 Luminex Corporation Instrument for cassette for sample preparation
US10214767B2 (en) 2006-12-27 2019-02-26 Luminex Corporation Instrument for cassette for sample preparation
US9248422B2 (en) 2010-02-23 2016-02-02 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US9931636B2 (en) 2010-02-23 2018-04-03 Luminex Corporation Apparatus and method for integrated sample preparation, reaction and detection
US8602750B2 (en) * 2010-04-20 2013-12-10 Koganei Corporation Liquid supply device
US20110253750A1 (en) * 2010-04-20 2011-10-20 Koganei Corporation Liquid supply device
US11293416B2 (en) * 2017-09-21 2022-04-05 Hartmann's, Inc. Precision fluid dispensing device

Also Published As

Publication number Publication date
GB1256746A (de) 1971-12-15
DE1902297B2 (de) 1971-05-19
DE1902297A1 (de) 1969-09-11
JPS4947477B1 (de) 1974-12-16
SE327840B (de) 1970-08-31
FR2000695A1 (de) 1969-09-12
NL6900596A (de) 1969-07-29

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