US3545935A - Disposable reaction container for an automatic chemical analyzer - Google Patents

Disposable reaction container for an automatic chemical analyzer Download PDF

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US3545935A
US3545935A US778185A US3545935DA US3545935A US 3545935 A US3545935 A US 3545935A US 778185 A US778185 A US 778185A US 3545935D A US3545935D A US 3545935DA US 3545935 A US3545935 A US 3545935A
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piston
storage
reagent
compartment
reaction
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US778185A
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William J Kearns
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Xerox Corp
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Xerox Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • Disposable reaction container comprising a lower section having at least one compartment for the admixing of materials added thereto; an upper storage section having at least one separate, rigid cylindrical reagent storage chamber associated with each reaction compartment, each of said storage chambers having a piston therein for dispensing tableted reagents therefrom; and restraining means to prevent the premature movement of prepackaged reagents from the plurality of storage chambers.
  • Each piston has a cavity in the lower portion thereof suitable for surrounding a tableted reagent within the storage chamber during the downward stroke of the piston, whereby the force necessary to break the restraining means, normally in the form of a thin plastic layer, is not transmitted to the restraining means through the tableted reagent.
  • This invention relates to automatic chemical analysis and, more particularly, to the automatic chemical analysis of body fluids, such as blood, urine, etc.
  • an automated chemical analytical system including a plurality of diiferent disposable reaction containers, a magazine for the storage of the plurality of different reaction containers, a station for the addition of sample material to the reaction container, a mixing and incubation station wherein the reaction mixture is maintained in the disposable container for a period of time sufficient to culminate the chemical reaction, a detection station wherein the analytical data is obtained by monitoring one or more of the physical properties of the reaction mixture, a disposal station wherein the disposable reaction container is eliminated from the system, and means to transport the disposable reaction container from its storage area in the magazine through the system to the disposal station.
  • the heart of the system is the disposable reaction container which, in its broad aspects, has at least one lower compartment for the admixing and reaction of reagents and sample, and an upper section having a plurality of reagent storage chambers in communication with each reaction compartment.
  • At least one wall or end portion of the reaction compartment may be optically transparent so that upon completion of the desired chemical reaction the compartment can be utilized as a cuvette for optical analysis.
  • none of the walls need be optically transparent as a probe photometer, such as the one disclosed in Gale 3,164,663, may be inserted into the reaction mixture and electromagnetic radiation from a source passed through a radiation conductor, the reaction mixture and back through the radiation conductor to a detection means, without the necessity of passing through the compartment walls.
  • the disposable reaction container in this application has a flexible lower compartment, i.e., one having at least one flexible wall, so that during analysis a light source and a detection means pressed against the flexible wall or walls defining the lower cuvette(s) will cause the wall(s) to yield a distance sufiicient to define a fixed optical path between the light source and the detection means through the reaction mixture.
  • the automatic analytical apparatus includes monitoring means having a light source and a means responsive to the variations in light transmittance caused by different concentrations of a constituent under analysis in the reaction mixture. The light source and the responsive means are pressed against opposite sides of the reaction compartment or cuvette during analysis to define a fixed optical path through the reaction mixture.
  • an automatic analytical apparatus having the optical path defining means built into the detection station.
  • reaction container can be mass produced and disposed of after use without significant additional cost.
  • the lower section of the disposable reaction container comprises positioned walls adapted to channel the material added thereto to a portion of the lower compartment defined by a substantially rectangular volume. It is through this rectangular volume that optical analysis is made.
  • a still lower compartment can be provided for the storage therein of a magnetic stirring bar so that thorough mixing of added materials could be achieved through use of external urging means magnetically coupled to the magnetic stirring bar.
  • the disposable container shown in Ser. No. 645,665, now abandoned is considered typical of containers most easily adapted for use in an automated analytical system.
  • the container has two reaction compartments, a plurality of reagent storage chambers associated with each reaction compartment, and restraining means adapted to prevent the premature movement of prepackaged reagents from their respective storage chambers.
  • the restraining means is in the form of a thin layer heat sealed between the lower reaction compartment section and the upper reagent storage section.
  • Liquid storage is not as desirable because there is a greater propensity towards chemical reaction, either with the storage wall or with material permeating therethrough. Additionally, liquid materials are generally known to be more sensitive to light and other portions of the electromagnetic spectrum and, therefore, degrade faster unless adequate filters are provided to eliminate deleterious radiation.
  • Tableting is less subject to these disadvantages so that increased storage times can be attained.
  • the reagents can be tableted either singly or in combination with other compatible reagents. This is preferred to storing two or more powdered reagents together since powder storage inherently provides an extreme amount of surface area available for chemical reaction.
  • each reagent which is chemically incompatible with other reagents can be prepackaged in its own tableted composition suitable for storage in its own, separate storage chamber.
  • severe dust, cross-contamination, and electrostatic problems exist when a plurality of different powdered chemicals are being deposited into reagent storage chambers which are but a fraction of an inch apart.
  • the tableted reagents are extremely hygroscopic and, therefore, must be prepared, packaged and maintained under dry, non-humid conditions.
  • the restraining layer also called the restraining layer
  • Yet a still further object of the present invention is to provide a novel, injection molded upper storage section of a disposable reaction container having a plurality of storage cylinders, each cylinder having a piston therein for shearing the restraining layer provided between the storage cylinders and a reaction compartment section adjacent thereto.
  • Yet a still further object of the present invention is to provide novel pistons for use in conjunction with the novel upper storage section of the present invention.
  • the improved disposable reaction container has a uniquely configurated upper section for the storage of reagents, preferably tableted reagents, added thereto.
  • the storage section of the present invention comprises a rigid member having at least one reagent storage chamber formed therein, and a lower layer or flange surrounding the opening(s) therethrough at the bottom of the substantially vertically extending rigid storage cylinder(s). Restraining means are provided adjacent the openings at the bottom of the storage cylinders to prevent the premature movement of stored reagents therefrom.
  • the restraining means comprises a thin plastic layer securely bonded to the lower portion of the flange encircling the lower openings of the storage cylinders.
  • a piston is positioned within each storage chamber so that upon downward movement thereof, the restraining means will be broken whereby material stored within the storage chamber will be dispensed therefrom.
  • the storage section of the present invention is utilized in combination with a lower reaction compartment, for example, the lower reaction compartment described in application Ser. No. 764,850, filed Oct. 3, 1968, to provide a complete disposable testing unit suitable for conducting a complete chemical analysis therein.
  • a plurality of lower reaction compartments and an upper storage section having at least one storage cylinder associated with each reaction compartment.
  • the storage cylinders can have individual walls, such as shown in FIGS. 1-3, or a plurality of the storage cylinders can be enclosed in a block of material as shown, for example, in FIGS. 4 and 5.
  • a hole or channel can be provided through the upper storage section for the addition therethrough of material, such as sample, water, etc., to the lower reaction compartment associated therewith.
  • Restraining means are provided between the upper storage section and the lower reaction compartment to prevent the premature movement of the stored materials from their respective storage cylinders.
  • the restraining means comprises a thin plastic layer, of equal dimension to the flange surrounding the opening(s) in the bottom of the storage cylinder(s), to which both the upper storage section and the lower reaction compartment section are firmly bonded on opposite sides thereof. Downward movement of the piston in the storage cylinder destroys the restraining means thereby permitting dispensing of the stored material.
  • the upper storage section of the present invention differs from previous storage section configurations in that the upper portion of the storage cylinder is not closed to the atmosphere, means must be provided to prevent the admission of deleterious components, such as water vapor, etc., which might adversely affect the chemical stability and activity of the stored material.
  • this can be achieved, for example, by bonding a separate barrier layer or membrane across the upper portion of the storage section [i.e., over the top openings of the storage cylinder(s)], or by adding a hot melt coating to the top of each storage chamber, etc.
  • the piston placed in the storage cylinder can take on many shapes and forms.
  • the essential feature which is common to all forms of the piston is that it have a cavity in the lower portion thereof which will enclosed the stored reagent, preferably in tablet form, as the piston is moved downwardly in the cylinder.
  • the outer circumference of the lower portion of the piston will thereby cut through the restraining means prior to the time when the portion of the piston at the top of the cavity contacts the tableted reagent.
  • the tablet is not crushed during the dispensing operation and, as such, is termed a passive tablet since it is not utilized to break the restraining means.
  • force was always applied on the top of the storage chamber for inversion thereof during the dispensing operation.
  • FIG. 1 is a top view of the reagent storage section of the present invention
  • FIG. 2 is a side sectional view of the reagent storage section of FIG. 1 taken along lines 2-2 thereof;
  • FIG. 3 is a bottom view of the reagent storage section of FIG. 1;
  • FIG. 4 is a top view of an alternate embodiment of the reagent storage section of the present invention.
  • FIG. 5 is a side sectional view of the reagent storage section of FIG. 4 taken along lines 5-5 thereof;
  • FIGS. 6-9 are side sectional views of pistons suitable for use within the storage cylinders of the reagent storage sections.
  • FIGS. 10 and 11 are side sectional views of a single storge cylinder showing the two primary piston positions during reagent storage and dispensing.
  • FIGS. 1-3 there is shown a disposable reaction container upper section 10 having a pluarlity of storage cylinders 12 for the storage of prepackaged reagent therein.
  • Storage section 10, including the plurality of storage chambers 12 is formed as a rigid unitary member having the configuration to be described hereinafter.
  • each storage chamber is cylindrical in form having a cylindrical sidewall 14 open both at the top 16 and at the bottom 18.
  • a step or shoulder 20 adapted to prevent a piston inserted therein from being dispensed through opening 18 during the reagent addition operation.
  • the reagent storage cylinders are paired into two groups of four cylinders each, each group being positioned over a lower reaction compartment (not shown) in the manner as shown in copending application Ser. No. 645,665, now abandoned.
  • a flange 22 Surrounding the lower perimeter of upper section 10 is a flange 22 holding the reagent storage chambers together as an over-all unit and providing space 24 for the recording or storing of informational data thereon.
  • a small hole 26, centered between each group of four reagents storage cylinders 12, is provided in flange 22 through which sample material, or diluents such as water, etc. are added to the reaction compartment positioned therebeneath.
  • Polystyrene and polyvinyl chloride are two suitable materials from which the aforementioned storage sections can be mass produced by well-known injection molding techniques.
  • FIGS. 4 and 5 there is shown an alternate embodiment upper storage section of the present invention. Like numerals as found in FIGS. 1-3 indicate elements of the upper storage section common to embodiments.
  • the embodiment shown in FIGS. 4 and 5 differ from the embodiment shown in FIGS. 1-3 in three primary features. Initially, the plurality of storage cylinders are formed in a block of rigid material as opposed to having individual walls. This provides sufiicient surface area for the bonding of a barrier-membrane to the upper surface thereof. As shown, the side walls of the block are slightly tapered, such as at 32, so the storage section can more easily be removed from its assembly mold.
  • step 20 is angled to the side walls of the storage cylinder rather than being perpendicular thereto. This reduces the possibility of a cutting edge of the piston inadvertently being forced or locked into the right angle corner such that effective piston introduction and/or reagent dispensing cannot be achieved.
  • ribs can be provided encircling the lower perimeter of each storage cylinder to thereby clearly define that portion of the restraining layer which is to be severed by the piston during its downward stroke. Shearing of the restraining layer is confined to the appropriate area within the encircling rib and does not spread to other portions of the layer below other storage cylinders.
  • a rib can also be provided substantially midway between adjacent reaction compartments to assist in maintaining the separate integrity of each reaction compartment and its associated plurality of reagent storage chambers.
  • pistons suitable for use within the reagent storage cylinders there are shown pistons suitable for use within the reagent storage cylinders.
  • piston has a substantially cylindrical side wall 42 which is stepped inwardly to provide a ledge 44 adapted to seat against step 20 within the reagent storage chamber. This inward step should be positioned such that when the piston is in its lowermost position (i.e., resting on step 20 in the storage cylinder), that portion of the piston extending into the reaction compartment does not interfere with optical analysis therethrough.
  • the upper portion of the piston is hollowed out to define a cavity 46 having an interior side wall 48 and bottom wall 50.
  • Side wall 48 is inclined slightly to the vertical, for example at an angle of about 10, so that when a plunger is inserted into the cavity it will be guided by side wall 48 to the lowest portion of the cavity, i.e., to bottom wall 50.
  • the bottom portion of the piston is also hollowed out to define a second cavity 52 having interior wall 54 and top wall 56.
  • Bottom Wall of cavity 46 and top wall 56 of cavity 52 are substantially parallel to thereby define a circular rib 58 connecting the opposite sides of substantially cylindrical wall 42.
  • Rib 58 should be of sufiicient strength so that application of force thereto, in a downward direction, will cause the desired piston movement.
  • side wall 54 angles downwardly and outwardly, for example at an angle of about 2", so that the opening at the bottom of the cavity defines a circle of larger diameter than the circle defined by top wall 56.
  • This slight outward taper insures that the lower leg 59 of side wall 42 contacts a reagent tablet in such a manner as not to crush the tablet before it is ejected from the storage chamber.
  • a tablet contacted by interior wall 54 will be guided further up into the cavity as the piston is lowered during the reagent addition operation.
  • the lower end of leg 59 is beveled upwardly and outwardly from its lowest point to define a cutting edge 60 for shearing through the restraining layer as the piston is lowered in the storage cylinder.
  • the angle formed by an extension of outer wall 64 and beveled wall 62 can be, for example, 30.
  • Beveled wall 62 is adapted to prevent seating of leg 59 against stop 20 within the reagent storage chamber. That is, as the piston is lowered, the slanted wall 62 will inherently cause the downwardly moving piston to be guided through opening 18 as opposed to having it seat against stop 20. Perfect alignment of the piston with opening 18 is thereby achieved.
  • Piston 70 as shown therein, also has a substantially cylindrical side wall 42 stepped inwardly at 44 to provide a stop to seat against shoulder 20 within the reagent storage cylinder for limiting the downward movement of the reagent dispensing piston.
  • the upper and lower portions of the piston are hollowed out to define cavities 46 and 52 having therebetween rib 58.
  • the exterior upper portion of side wall 42 is, however, flared outwardly at 72 to provide a better seal with the cylinder wall to prevent the admission of both air and ambient moisture.
  • the upper portion of wall 48 is flared outwardly to clearly define a much greater circular opening at the top of cavity 46 than either at the plane 76 where the flared portion 74 begins or at the bottom wall 50 of cavity 46. This greatly emphasized flaring of interior wall 48 more positively assures that the piston actuating means (not shown) will be directed against rib 58 during reagent dispensing.
  • the lower portion of the piston is similar to that described in FIG. 6 in that it has a lower leg 59 beveled upwardly and outwardly to define a similar cutting edge 60.
  • the wall portion 62 also defined thereby functions to inherently guide the piston through opening 18.
  • the lower portion differs from that shown in FIG. 6, however, by
  • notch 80 cut out of a portion of leg 59.
  • the vertical dimension of notch 80 is such that the portion of the restraining layer therebeneath is not severed during the dispensing operation. As shown, the notch extends substantially for the total height of cavity 52.
  • the vertical distance between the top edge 82 of notch 80 and step 44 and piston wall 42 is less than the vertical distance between stop on the storage cylinder wall and the lowermost surface of flange 22 encircling the lower perimeter of the storage chamber.
  • a flap will result whereby the small portion of the restraining layer below the storage cylinder will not be completely severed from the remainder thereof.
  • the dangling flap will not interfere with either mixing of the materials added to the reaction compartment (including reagent tablets to be dissolved) or optical analysis therethrough.
  • the essential feature of the notch is that it prevents complete severance of the restraining layer below the storage cylinders.
  • Cavities 46 in the pistons of FIGS. 6 and 7 can be utilized for the addition of material, such as hot melt wax, thereto for providing a barrier to the admission of substance(s) which would have a deleterious effect on the activity, etc. of the reagent stored within the storage cylinder. Accordingly, such a material can be added to the cavity and overlap the interface between the piston wall and the cylinder wall to provide an effective seal.
  • material such as hot melt wax
  • piston 86 has a substantially cylindrical side wall 42 slanted inwardly at 44 to provide a stop adapted to seat against shoulder 20 within the reagent storage cylinder.
  • the lower portion of piston 86 is hollowed out to define cavity 52; however, there is no cavity in the upper portion of the piston.
  • the length of the piston is such that when cutting edge 60 is resting on the restraining means, the top surface 88 of the piston is substantially coplanar with the top surface of the reagent storage section.
  • this piston is used in conjunction with the storage section of FIGS. 4 and 5, it provides additional surface area for the bonding of a barrier-membrane across the upper surface of block 30.
  • Stop 44 is angled upwardly and outwardly, for example at an angle of 45, from leg 59 to side wall 42 and is most suitable for use in conjunction with a similarly angled stop 20 in the cylinder wall. Additionally, the lower portion of leg 59 is beveled upwardly and inwardly (as opposed to outwardly) from its lowermost point to define cutting edge for shearing through the restraining layer as the piston is lowered in the storage cylinder. The angle formed by the wall portions defining cutting edge 60 can be, for example, 30. Beveled wall 90 is adapted to assist in the positioning of a reagent table within cavity 52 during the downward stroke of piston 86. Additionally, by providing cutting edge 60 at the outermost point of leg 59, the restraining layer is severed or broken closer to the point where it is sealed to the bottom surface of flange 22.
  • cutting edge 60 is formed by beveling portions of leg 59 upwardly and outwardly from its lowermost point.
  • cutting edge 60 is defined by leg 59 and slanted wall 92. From that cutting edge, however, the lowermost portion of the piston is tapered at a slight angle, say on the order of 4 or 5, so that, at the side of the piston directly opposite therefrom, leg 59 terminates in a blunt wall 94 unsuitable for severing that portion of the restraining layer therebeneath.
  • the restraing layer is initially cut by cutting edge 60 substantially, but not completely, around the circle circumscribed by the piston. Since surface 94 is incapable of cutting through the restraining layer, that portion of the restraining layer beneath the piston is not completely severed from that portion of the restraining layer which remains bonded to flange 22. Accordingly, the restraining layer so severed does not interfere with the subsequent optical analysis. This particular modification eliminates the need for the notch as shown in FIG. 7.
  • the essential structural characteristic of the piston is that it have a cavity in the lower portion thereof suitable for surrounding, and not adversely contacting, the tableted reagent during the downward stroke of the piston.
  • Other disclosed features such as the upper cavity vs. the flat top, the right angle step vs. the slanted step, the inward bevel vs. the outward bevel, the tapered cutting edge vs. the notch, and other equivalent design features, etc. can be interrelated to define a piston not exactly described herein but which would be suitable for use in the present invention. All such design variations not specifically covered in the drawings are intended to be covered by the present invention.
  • a complete disposable container is taken from a supply magazine and passed to a sample addition station where the proper amount of sample diluted with distilled water is aliquoted into the reaction compartment, for example through hole 26.
  • the sample-holding container is then passed to a reagent addition station wherein reagents stored in the storage chambers are emptied into the appropriate compartments.
  • Reagent addition can be done in one operation or it can be done sequentially as it is necessary to complete the analytical procedure. If done sequentially, the addition can be done during or after incubation. In essence, reagents can be added any time prior to final detection as determined by the particular analytical procedure utilized.
  • the container is passed to a mixing station where it is maintained for a time sufiicient to ensure the dissolution of all solid materials in the liquid contained in the lower compartments.
  • the container next passes to an incubation station where appropriate reaction conditions are imposed upon the materials within the container for a time suflicient to complete the desired reaction which is then measured at a detection station. If necessary the package passes to further reagent addition, mixing and incubation stations as dictated by the analytical procedure. It is not necessary that the mixing and incubation stations be separate and distinct as it is contemplated that these operations may be performed in a single station.
  • light of appropriate wavelength is passed from a light source through the reaction mixture to detection means situated on the opposite side of the reaction mixture from the light source.
  • the amount of light transmitted (or, conversely, the amount of light absorbed) at the testing wavelength will be representative of the amount of the constituent under analysis in the test solution.
  • the disposable container as shown in the drawings is used in conjunction with a double-beam detection mechanism.
  • a solution of the material being tested with all the reagents which will bring the reaction mixture to the desired point for analysis.
  • the other compartment contains a solution of the material being tested in the absence of reagents.
  • one or more reagents can be added to this latter solution, provided the reagents do not carry the reaction to completion or do not adversely atlect, in any other way, the optical analysis.
  • This latter solution is called a critically incomplete blank and will enable the analytical system to compensate for the efifects of the sample and the reagents added thereto.
  • standard solutions are passed through the detection mechanism at intervals so that the latter can adjust for deviations which occur during operation.
  • a disposable container having three compartments, and the plurality of storage chambers associated with each compartment where reagents need be added, is provided for use with a triple-beam detection mechanism.
  • the standard solution can be injected into the disposable container at any point in the system prior to optical analysis and will obviate the need for passing distinct disposable container holding standards through the system.
  • standard solution-producing materials can be stored in the upper section, dispensed into the lower compartment and diluted to give the desired concentration.
  • the detection mechanism will analyze the standard and adjust for deviations from the known value. The analysis of the materials in the other two compartments is conducted in accordance with the teachings above.
  • light from the light source and light which has passed through the reaction mixture can be conducted to the disposable container and the detection means, re-
  • optical path is defined by the distance between the opposite walls of the lower compartment against which the light conduits, or the equivalent thereof, are in contact.
  • This optional form of optical analysis is shown in FIG. of Ser. No. 645,665.
  • higher than atmospheric pressure means can be positioned over the upper storage section so that a relatively inert gas can be admitted to the reaction compartment through the hole made in the upper section during sample addition.
  • the flexible walls will be bowed outwardly and can be made to contact accurately positioned optical path-defining means.
  • optical path defining means is now built into the detection station and, as would be expected, significantly less detection stations should be produced than disposable containers. Since a fixed optical path is defined by the detection station and will be the same for each container passing therethrough, highly accurate and reliable data can be obtained with this system.
  • optical analy sis can be used in a doubleor triple-beam detection mode, as described above.
  • the number of reagent tablets necessary will depend upon the particular analysis being prepackaged into the disposable container as well as the compatibility of the different reagents. In certain instances, it is possible to tablet more than one reagent in a single tablet. However, where it is contemplated that the disposable containers will be prepared long before their actual use, the compatibility of the reagents over this long period of time must clearly be established. If this cannot be done, then it is desirable to tablet the reagents separately. In turn, the number of storage chambers will depend upon the number of reagent tablets utilized.
  • FIGS. 10 and 11 The cooperation of the piston and the reagent storage section is more clearly set forth in FIGS. 10 and 11 where the dispensing of a tableted reagent is shown.
  • single storage cylinder 100 has a piston P positioned therein.
  • a barrier membrane 106 is bonded to the top surfaces of piston P and the walls of storage cylinder 100.
  • the piston is shown in its uppermost position in the storage cylinder whereby lower cavity 52 partially surrounds and encloses reagent tablet T with the cutting edge 60 at the lower portion of the piston only in slight contact with restraining layer 92.
  • the present preferred form of the lower reaction compartment section is as shown in co-pending application Ser. No. 764,850, filed Oct. 3, 1968, assigned to the assignee of the present invention, and incorporated herein by reference.
  • a side view of such a reaction compartment section (showing only a single reaction compartment) is as shown at 104 in FIGS. 10 and 11.
  • the reaction compartment has a unique configuration adapted to promote coupling of the ultrasonic energy from an ultrasonic generating means adjacent thereto with materials held within the reaction compartment.
  • the unique design includes a fiat portion at the bottom of each reaction compartment, and upwardly extending bottom wall portions connecting said fiat portion to the four side walls of the reaction compartment whereby said flat portion is positioned as the lowest point within the reaction compartment.
  • Solid materials, especially in tablet form, added to the reaction compartment will be preferentially positioned over the fiat portion such that highly effective coupling is attained when the flat portion is properly aligned over the adjacent ultrasonic generating means.
  • a more complete discussion of that design is given in the aforementioned co-pending application and to the extent necessary to complete the disclosure of this application is incorporated herein by reference.
  • Other lower reaction compartment sections can be utilized in conjunction with the reagent storage section of the present invention; for example, another suitable lower reaction compartment section is disclosed in Ser. No. 645,665 now abandoned; which is also incorporated herein by reference.
  • the upper section of the present invention has a flange extending about the lower perimeter of the plurality of reagent storage chambers.
  • One side of this flange which extends the length of the disposable reaction container is slightly wider than the border that encircles the remainder of the upper storage section.
  • the flange which encircles the adjacent lower sec tion also has such a wider portion of similar dimensions.
  • each member is formed out of a plastic material which can be heat sealed to the adjacent member to provide an exceptionally strong bond which cannot be broken under normal use.
  • the wider portion of the flange encircling the perimeter of the upper storage section is sufficiently wide so that a suitable code can be provided thereon.
  • Any suitable type of coding can be placed on the code area to indicate or record any information which desirably should be known during a chemical analysis, such as the actual test which has been pre-stored in the particular disposable reaction container, patient number, instructions for the associated automatic analytical apparatus and system, analytic results, etc.
  • Typical codes include binary coding in the form of light and dark areas, magnetic coding, etc.
  • a storage section comprising a rigid member having at least one substantially vertical, cylindrical storage chamber formed therein, each of said storage chambers having a shoulder on the interior wall thereof for limiting the downward movement of a piston positioned therein, and a flange encircling the bottom opening of each of said storage chambers; restraining means securely bonded to the lower surface of said flange for preventing the premature movement of prepackaged reagents from each of said storage chambers; and a piston positioned in each of said storage chambers and freely movable within said storage chamber along the longitudinal axis thereof, each of said pistons having a cavity in the lower portion thereof which is adapted to enclose a reagent tablet therein as said piston is moved downwardly within said storage chamber, said cavity being of such vertical dimension that the reagent tablet therein is not contacted by the upper portion of said piston prior to the time when the lower portion of said piston creates an opening in that portion of said restraining means underlying said storage chamber.
  • the storage section of claim 1 further including a dry tabletted reagent stored in at least one of said storage chambers.
  • the storage section of claim 2 further including barrier means disposed over the upper portion of said storage cylinders for preventing the admission of deleterious materials thereto.
  • a storage section comprising a rigid member having a plurality of cylindrical storage chambers formed therein, each of said storage chambers having top and bottom openings and a shoulder on the interior wall thereof for limiting the downward movement of a movable piston positioned therein, a flange encircling said bottom openings of said storage cylinders; restraining means firmly bonded to the lower surface of said flange; and a piston positioned in each of said storage cylinders and freely movable within said storage cylinder along the longitudinal axis thereof, each of said pistons having a cavity in the lower portion thereof which is adapted to enclose a reagent tablet therein as said piston is moved downwardly within said storage cylinder, said cavity being of such vertical dimension that the reagent tablet therein is not contacted by the upper portion of said piston prior to the time when the lower portion of said piston creates an opening in that portion of said restraining means underlying said storage cylinder.
  • the storage section of claim 5 further including an aperture in said flange for each group of cylinders, each of said apertures being positioned between said cylinders within the rectangle defined thereby.
  • the storage section of claim 4 further including a dry tabletted reagent stored in at least one of said storage cylinders.
  • the storage section of claim 4 further including barrier means positioned over the upper surface of said storage cylinders for preventing the admission of deleterious materials thereto.
  • each group of four storage cylinders are formed within a block of material such that individual exterior wall portions of said cylindrical storage chambers are not formed.
  • the storage section of claim 11 further including a shearable layer firmly bonded to the upper surface of said block of material.
  • a storage section comprising a rigid member having a plurality of cylindrical storage chambers formed therein, said plurality of storage cylinders being sectioned into two groups of four cylinders each, the centers of said four cylinders in each of said groups being at the corners of a rectangle, each of said storage cylinders having top and bottom openings and a shoulder on the interior wall thereof for limiting the downward movement of: a piston positioned therein, a flange encircling said bottom openings of said storage cylinders, an aperture in said flange for each group of cylinders, each of said apertures being positioned between said cylinders within the rectangle defined thereby; restraining means firmly bonded to the lower surface of said flange; a piston positioned in each of said storage cylinders and freely movable within said storage cylinder along the longitudinal axis thereof, each of said pistons having a cavity in the lower portion thereof which is adapted to enclose a reagent tablet therein as said piston is moved downwardly within said storage cylinder, said
  • the storage section of claim 17 further including dry tabletted reagent stored in at least a portion of said storage cylinders.
  • a disposable reaction container comprising an admixing compartment section having at least one compartment for the admixing of materials added thereto, said lower section having a flange adjacent the upper perimeter thereof and encircling the opening at the top of each admixing compartment; a substantially rigid storage section securely mounted on said admixing compartment section, said storage section having a plurality of separate, substantially rigid cylindrical reagent storage chambers adjacent said opening at the top of each of said compartment, each of said storage cylinders having top and bottom openings and a shoulder on the interior wall thereof for limiting the downward movement of a piston positioned therein, and a flange encircling said bottom openings of said storage cylinders; restraining means to prevent the premature movement of prepackaged reagents from said plurality of storage cylinders; and a piston positioned in each of said storage cylinders and freely movable within said storage cylinder along the longitudinal axis thereof, each of said pistons having a cavity in the lower portion thereof which is adapted to enclose a
  • each admixing compartment is optically transparent so that upon completion of the desired chemical reaction, each compartment can be utilized as a cuvette for optical analysis.
  • said restraining means comprises a shearable layer sandwiched between said storage section and said admixing compartment section, said shearable layer being sufficiently strong so that it will shear only below a particular storage chamber when a prepackaged reagent is dispensed therefrom.
  • each of said pistons has a beveled cutting edge at the lower extremity thereof.
  • a piston comprising a unitary member having a cylindrical side wall and a cavity in the lower portion thereof, said cavity being defined by interior wall portions and an opening in the bottom wall of said piston, said opening having suflicient diameter and said cavity extending for a suflicient vertical distance to permit a tabletted material to be enclosed within said cavity during the downward stroke of said piston.
  • a piston comprising a cylindrical member, the diameter of the upper portion of said member being greater than the diameter of the lower portion thereof to thereby define upper and lower cylindrical wall portions and an intermediate wall portion adapted to limit the downward movement of the piston, a cavity in the lower portion of said piston, said cavity being defined by interior wall portions and an opening in the bottom wall of said piston, said opening having a diameter slightly less than the diameter of said lower portion, said cavity extending for a vertical distance sufficient to permit a tableted material to be enclosed within said cavity during the downward stroke of said piston, and said cylindrical lower wall portion being beveled at the bottom of said piston to define a distinct cutting edge about said opening.
  • the piston of claim 29 further including a cavity in the upper portion of said piston, said upper cavity and said lower cavity defining a substantially horizontal rib therebetween.
  • a disposable reaction container comprising an admixing compartment section having at least one compartment for the admixing of materials added thereto, said admixing compartment section having a flange adjacent the upper perimeter thereof and encircling the opening at the top of each admixing compartment; a substantially rigid storage section securely mounted on said admixing compartment section, said storage section having at least one separate, substantially rigid reagent storage chamber adjacent said opening at the top of each of said compartments, each of said storage chambers having a bottom opening and a shoulder on the interior wall thereof for limiting the downward movement of a movable piston positioned therein, and a flange encircling said bottom openings of said storage chambers; restraining means to prevent the premature movement of prepackaged reagents from each of said storage chambers; and a piston positioned in each of said storage chambers and freely movable within said storage chamber along the longitudinal axis thereof, each of said pistons having a cavity in the lower portion thereof which is adapted to enclose a reagent
  • each admixing compartment is optically transparent so that upon completion of the desired chemical reaction, each compartment can be utilized as a cuvette for optical analysis.
  • each of said storage chambers has a top opening and barrier means positioned over the upper surface of each of said storage chambers for preventing the admission of deleterious materials thereto.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Medicinal Preparation (AREA)
US778185A 1968-11-22 1968-11-22 Disposable reaction container for an automatic chemical analyzer Expired - Lifetime US3545935A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US77818568A 1968-11-22 1968-11-22

Publications (1)

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US3545935A true US3545935A (en) 1970-12-08

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US778185A Expired - Lifetime US3545935A (en) 1968-11-22 1968-11-22 Disposable reaction container for an automatic chemical analyzer

Country Status (12)

Country Link
US (1) US3545935A (de)
JP (1) JPS5323718B1 (de)
BE (1) BE741890A (de)
BR (1) BR6912180D0 (de)
CA (1) CA933494A (de)
CH (1) CH500010A (de)
DE (1) DE1957735C3 (de)
ES (1) ES373794A1 (de)
FR (1) FR2023873A1 (de)
GB (1) GB1257338A (de)
NL (1) NL6917231A (de)
SE (1) SE353399B (de)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3715189A (en) * 1970-06-15 1973-02-06 Secretary Of The Treasury Qualitative analysis device
US4215092A (en) * 1976-04-08 1980-07-29 Osmo A. Suovaniemi Apparatus for liquid portioning and liquid transferring
US4720374A (en) * 1985-07-22 1988-01-19 E. I. Du Pont De Nemours And Company Container having a sonication compartment
US4863693A (en) * 1984-08-21 1989-09-05 E. I. Du Pont De Nemours And Company Analysis instrument having a blow molded reaction chamber
US20140260621A1 (en) * 2013-03-15 2014-09-18 Inficon, Inc. High capacity monitor crystal exchanger utilizing an organized 3-d storage structure
EP2934730A4 (de) * 2012-12-21 2016-11-23 Leica Biosystems Melbourne Pty Verfahren zur herstellung eines reagens an bord eines instruments

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3476515A (en) * 1966-04-26 1969-11-04 Du Pont Analytical test pack and process for analysis
US3480398A (en) * 1967-12-26 1969-11-25 Xerox Corp Chemical package

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3476515A (en) * 1966-04-26 1969-11-04 Du Pont Analytical test pack and process for analysis
US3480398A (en) * 1967-12-26 1969-11-25 Xerox Corp Chemical package

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3715189A (en) * 1970-06-15 1973-02-06 Secretary Of The Treasury Qualitative analysis device
US4215092A (en) * 1976-04-08 1980-07-29 Osmo A. Suovaniemi Apparatus for liquid portioning and liquid transferring
US4863693A (en) * 1984-08-21 1989-09-05 E. I. Du Pont De Nemours And Company Analysis instrument having a blow molded reaction chamber
US4720374A (en) * 1985-07-22 1988-01-19 E. I. Du Pont De Nemours And Company Container having a sonication compartment
EP2934730A4 (de) * 2012-12-21 2016-11-23 Leica Biosystems Melbourne Pty Verfahren zur herstellung eines reagens an bord eines instruments
US20140260621A1 (en) * 2013-03-15 2014-09-18 Inficon, Inc. High capacity monitor crystal exchanger utilizing an organized 3-d storage structure
US9182378B2 (en) * 2013-03-15 2015-11-10 Inficon, Inc. High capacity monitor crystal exchanger utilizing an organized 3-D storage structure

Also Published As

Publication number Publication date
FR2023873A1 (de) 1970-08-21
DE1957735B2 (de) 1974-01-03
CA933494A (en) 1973-09-11
BE741890A (de) 1970-05-19
CH500010A (de) 1970-12-15
BR6912180D0 (pt) 1973-02-01
JPS5323718B1 (de) 1978-07-17
SE353399B (de) 1973-01-29
ES373794A1 (es) 1972-02-01
DE1957735C3 (de) 1974-07-25
GB1257338A (de) 1971-12-15
NL6917231A (de) 1970-05-26
DE1957735A1 (de) 1970-06-11

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