US3452924A - System and method for washing blood and the like - Google Patents
System and method for washing blood and the like Download PDFInfo
- Publication number
- US3452924A US3452924A US430118A US3452924DA US3452924A US 3452924 A US3452924 A US 3452924A US 430118 A US430118 A US 430118A US 3452924D A US3452924D A US 3452924DA US 3452924 A US3452924 A US 3452924A
- Authority
- US
- United States
- Prior art keywords
- tube
- washing
- blood
- centrifuge
- tubes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3692—Washing or rinsing blood or blood constituents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
- A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Definitions
- This invention relates to a method and apparatus for continuously washing blood or other biological cells in suspension by passing a saline or other Washing solution through said suspension under centrifugation to remove contaminating proteins or other materials attached to the cells.
- the apparatus herein may also be used for washing other types of both organic and inorganic particles in various suspending media, as may be required or necessary.
- the washing of blood has been accomplished by a batch process in which a vial of sterile blood has added to it a first volume of saline washing solution, after which ⁇ the resultant mixture is vigorously shaken manually to effect mixing, and then inserted into a centrifuge. After the vial has been centrifuged, the saline solution together with some plasma contaminants are withdrawn, and a second fresh quantity of saline solution is added.
- the foregoing process is repeated at least four times to achieve the desired purification. This entire processing takes as much as 'four hours or more.
- One undesirable byproduct of this batch process is that the long time of processing causes the blood cells to exude a number of mucoid substances which contaminate the sera to which these cells might be added for absorptive purposes.
- the present invention overcomes these drawbacks of the batch process by teaching a system of balanced hydrodynamic centrifugal fluid columns in which the saline washing fluid component is continuously interjected into a plurality of centrifugal acceleration regions, so that a counterow of blood cells and washing solution simulate the previous shaking operation, thereby combining the mixing, washing, and centrifugal separation processes, and reducing the processing time to the order of one-half hour so as to obviate the exudation of mucoids, and to minimize t-he technician-hours spent in processing the blood.
- An additional feature of the invention provides sterile means to transfer the cleansed blood cells from the centrifugal gradient chambers into a sterile storage container.
- Another object of the invention is to provide a design of centrifugal gradient chamber which will permit the Iintroduction at a plurality of acceleration regions of a mixture of blood and washing solution while continuously withdrawing the effluent.
- Still another object of the invention is to provide a hydraulic circuit in which a sterile blood bag, a sterile washing solution reservoir, ⁇ a positive displacement pump, a balanced column rotating joint continuous flow centrifuge, a centrifugal gradient washing chamber, and an exhaust duct can be interconnected to effect the efficient Washing of the blood cells or other biological cells.
- disposable sterile blood bags may be utilized and the complete system may be maintained in a sterile condition from -the input end to the output end.
- a tfurther object is to provide a design of auxiliary sterilizable accessory ducts for the inlet and outlet tubes of the Iforegoing acceleration gradient washing chamber which rwill expedite the sterile transfer of the processed blood cells to a storage container.
- rFIGURE 1 is a diagrammatic view of an embodiment of the subject blood or cell washing system, showing the hydraulic circuit;
- FIGURE 2 is a side view, partly in section, ont the centrifugal acceleration gradient chamber of the subject invention
- FIGURE 3 is an enlarged, fragmentary view of the lower portion of the inlet gradient injection or distributor tube, a portion of which is shown in FIGURE 2, and which illustrates the spaced apart distributor ports as Well as the restrictive end orifices therein;
- FIGURE 4 is a side view showing the installation of the auxiliary intake air filter and exhaust needle with its guard, preparatory to the transfer of washed blood to its storage container;
- FIGURE 5 is a somewhat smaller wiew of the foregoing transfer assembly for gravity transfer of Washed blood cells to its storage bottle.
- plastic bag 21 contains the sterile blood specimen, which typically might have a volume of from to 400 ml., said bag 21 being suspended at the highest point in the system to generate a hydrostatic head which insures that the blood be deposited into the centrifuge early in the washing cycle. Bag 21 is sterilized and evacuated prior to being lled with the blood sample.
- a reservoir bottle 22 for the washing solution is provided With a stopper 23 through which are sealed a short air intake tube 24 and a long deep outlet tube 26.
- Intake tube 24 communicates with the outside atmosphere through an air filter 27 to maintain the sterility of the system.
- the washing solution which will typically be a hypertonic saline solution having a volume of four to ve times that of the blood sample, is drawn from the bottom of bottle 22 via tubes 26, 28 and 29, at a controlled rate by the action of the positive displacement electric motor driven pump 31.
- a useful type of pump for this purpose is one which uses a pair of spaced apart rotating rollers on a semi-circular track where they progressively collapse the wall of resilient rubber tube 29 so as positively to displace the fluid without the possibility of contaminating it.
- the blood from bag 21 is drawn by pump 31 via tube 32 and the tubular junction fitting 33, where the blood and saline washing solution mix, to pass together through pump 31 and tube 29.
- the mixture then passes through a flow meter 34, the series connecting tubes 36, 37 and 38, thence entering the electrically motor driven rotating-joint continuous iiow centrifuge rotor 39.
- centrifuges which are well known in the art, are based on the principle of balanced hydrodynamic columns, in the sense that incoming liquid, such as that entering via tube 38, is passed through a rotating seal into a centrifugal chamber or tube 44 having a closed outer end or bottom through a path which carries it into progressively higher centrifugal accelerations.
- the outlet column also passes through a separate rotary seal and hydraulically joins the inlet column in the centrifuging chamber. Consequently, except for the difference in density between the incoming and outgoing columns, there is a major balancing of the hydrodynamic columns, thereby permitting the continuous flow into and out of the high centrifugal field forces.
- the motivating force which causes fiow of fluid into and out of the centrifugal field is hydraulic head; i.e., a preponderance of fiuid head in the inlet lines over that in the outlet.
- the incoming fluid is conducted down the rotating axis of the centrifuge to a small central chamber where it is distributed to all the tubes from a point as near the axis of rotation as possible.
- the outlet tube feeds to a chamber also concentric with the axis, but whose periphery is radially further away.
- the difference, in these two resultant radial columns, then, is the hydraulic head which effects continuous iiow through the rotating centrifuge rotor.
- centrifuge tubes 44 are used in diametrically opposite pairs, and by a double manifold (not shown) the inlet tubes 48 of the several tubes 44 are connected together, and the outlet tubes 49 of these tubes are connected together.
- the effluent from the continuous flow centrifuge 39 is passed via serially connected tubes 41, 42 and 43, to a waste discharge.
- the entire centrifuge 39 together with its motor 46 is housed within a temperature controlled chamber 47 which may also be maintained in sterile condition.
- Centrifuge tubes 44 extend radially outwardly and downwardly from the axis of rotation of rotor 39. As said tubes 44 move around said axis of rotation, centrifugal force fields having radial gradients of acceleration are set up in said tubes, the centrifugal forces increasing in different region of said tubes depending upon their distances therein from said axis of rotation.
- FIGURE 2 shows the detailed construction of the subject acceleration gradient centrifuge tube 44
- a circular compression element 51 having a central upwardly extending threaded shaft 52 which extends freely through a central aperture in circular cap 53 which extends over and abuts the upper end of centrifuge tube 44.
- Cap 53 has an annular collar 54 which extends inwardly into the chamber of tube 44.
- Compression element 51 has an annular recess which accommodates a resilient O-ring 55.
- O-ring 55 is expanded by the increased pressure between compression element 51 and collar 54 to effect a seal between the inner wall of centrifuge tube 44 and the assembly of said compression element and cap.
- inlet tube 48 and outlet tube 49 are secured and sealed to the compression element 51 and extend freely through suitable apertures, not shown, in cap 53.
- Outlet tube 49 terminates flush with the inner surface of element 51 while inlet tube 48 continues nearly to the bottom end of the chamber of centrifuge tube 44.
- Inlet tube 48 is constricted at its bottom end to form one or two small terminal ports 57 (see FIGURE 3).
- tube 48 is provided with groups of spaced apart radial distributor ports 58.
- Distributor ports 58 are not only located in spaced apart gradients of centrifugal fields, but their respective locations also facilitate good dispersion and mixing of the wash fluid with the specimen cells. Depending upon the type and size of cells and other particulate materials that are to be washed and upon the nature of the washing medium, the sizes of distributor ports 58 may be selected and determined to provide for optimum efiiciency of the washing process. The size of distributor ports 58 may also be selected and determined to control the rate of dispersion and velocity at which the materials flow through tube 44.
- the dimensions of the holes 58 are approximately drill size 60, but it is contemplated that other drill sizes may be selected for said holes in adapting the apparatus for different types of materials to be processed thereby.
- the centrifuge motor 46 is deenergized to stop the rotor and the centrifuge tubes 44 are removed.
- an air filter 61 (FIGURE 4) is attached by a sterile tube 62 to tube 48 while a hollow sterile needle 63 with a protective cover 64 is connected to tube 49 by the sterile tube 66.
- Tube 44 is then clamped in a support pedestal 67, cover 64 is removed, and the needle 63 is caused to pierce the resilient cap 68 enclosing storage bottle 59. Gravity will then complete the transfer.
- the method of washing blood cells or the like in a liquid washing solution comprising the steps of forming a mixture of blood with a liquid washing solution having less density than said blood, moving a radially extending centrifuge tube with a closed outer end around an axis of rotation, introducing said mixture into said moving centrifuge tube at a plurality of radially spaced apart regions therethrough having differing values of centrifugal acceleration whereby the blood cells in said blood move in an outward direction in said centrifuge tube in counterflow to the movement of said washing solution, and withdrawing the effluent washing liquid from said centrifuge tube at a region of least centrifugal acceleration.
- a centrifuge structure for washing biological cells such as blood or the like comprising a rotor, a plurality of centrifuge tubes having closed outer ends arrayed radially in said rotor, means for continuously introducing materials to be centrifuged into each of said centrifuge tubes as the latter move around the axis of rotat-ion of said rotor, and outlet tube for continuously removing eluents from said centrifuge tubes during said rotation, said introducing means comprising an inlet tube extending into the interior and substantially throughout the length of each centrifuge tube and a plurality of spaced apart ports on said inlet tube for introducing said materials into the respective centrifuge tube at spaced apart regions therethrough where different degrees of centrifugal acceleration are engendered during rotation of said rotor.
- a continuous Washing system for biological cells in suspension according to claim 3 and further comprising a container for said suspension, a washing solution container', an air inlet duct including an air lter communicating with said washing solution container, a positive displacement pump, first duct means communicating between the inlet of said pump and both said suspension container and said solution container, second duct means communicating between the output of said pump and the said inlet tube of said centrifuge tube, and third duct means communicating between said outlet tube of said centrifuge tube and the exterior of said centrifuge.
Landscapes
- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Cardiology (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Centrifugal Separators (AREA)
- External Artificial Organs (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US43011865A | 1965-02-03 | 1965-02-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
US3452924A true US3452924A (en) | 1969-07-01 |
Family
ID=23706138
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US430118A Expired - Lifetime US3452924A (en) | 1965-02-03 | 1965-02-03 | System and method for washing blood and the like |
Country Status (3)
Country | Link |
---|---|
US (1) | US3452924A (de) |
DE (1) | DE1617782C3 (de) |
GB (1) | GB1128303A (de) |
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3977598A (en) * | 1973-05-24 | 1976-08-31 | Mcdonald Bernard | Centrifuge tube |
US3982691A (en) * | 1974-10-09 | 1976-09-28 | Schlutz Charles A | Centrifuge separation and washing device and method |
DE2612990A1 (de) * | 1975-03-27 | 1976-10-21 | Baxter Laboratories Inc | Stroemungssystem fuer die bearbeitung von zellen in einer zellbearbeitungszentrifuge |
US4049192A (en) * | 1976-11-01 | 1977-09-20 | Union Carbide Corporation | Blood washing method using a saline wash solution of varying concentration for use with blood washing apparatus |
US4069968A (en) * | 1976-11-01 | 1978-01-24 | Union Carbide Corporation | Disposable tubing harness for use with blood washing apparatus |
US4135883A (en) * | 1977-08-29 | 1979-01-23 | Bio-Dynamics Inc. | Blood analyzer system |
EP0072201A2 (de) * | 1981-08-05 | 1983-02-16 | Ortho Diagnostic Systems Inc. | Waschvorrichtung für Partikel und Verfahren zur Benutzung derselben |
FR2559075A1 (fr) * | 1984-02-07 | 1985-08-09 | Stephan Nees | Procede et dispositif pour la separation de particules, systemes de cellules biologiques et colloides par centrifugation |
US4804363A (en) * | 1986-07-16 | 1989-02-14 | Autologous Blood Corporation | Apparatus and method for storing and processing blood |
USRE33924E (en) * | 1986-07-16 | 1992-05-12 | Autologous Blood Corp. | Apparatus and method for storing and processing blood |
US5704889A (en) * | 1995-04-14 | 1998-01-06 | Cobe Laboratories, Inc. | Spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus |
US5704888A (en) * | 1995-04-14 | 1998-01-06 | Cobe Laboratories, Inc. | Intermittent collection of mononuclear cells in a centrifuge apparatus |
US6197579B1 (en) | 1997-02-14 | 2001-03-06 | Dendreon Corporation | Cell washing device and method |
US20070118063A1 (en) * | 2005-10-05 | 2007-05-24 | Gambro, Inc | Method and Apparatus for Leukoreduction of Red Blood Cells |
US20080096750A1 (en) * | 2006-10-20 | 2008-04-24 | Navigant Biotechnologies, Llc | Methods for Washing a Red Blood Cell Component and for Removing Prions Therefrom |
US20080147240A1 (en) * | 2006-12-19 | 2008-06-19 | Gambro Bct Inc. | Apparatus for separating a composite liquid with process control on a centrifuge rotor |
US20100291534A1 (en) * | 2009-05-12 | 2010-11-18 | National Central University | Methods and Systems for Isolating, Ex Vivo Expanding and Harvesting Hematopoietic Stem Cells |
EP2371943A1 (de) * | 2008-12-25 | 2011-10-05 | Olympus Corporation | Verfahren zum waschen von zellen |
CN113444628A (zh) * | 2021-05-28 | 2021-09-28 | 电子科技大学 | 一种组织块清洗装置及其清洗方法 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113000230A (zh) * | 2021-02-24 | 2021-06-22 | 安徽中科中佳科学仪器有限公司 | 一种杀菌消毒自清洁的实验室医用离心机 |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2758597A (en) * | 1955-01-24 | 1956-08-14 | Cutter Lab | Blood transfusion apparatus |
US2775240A (en) * | 1953-02-09 | 1956-12-25 | Abbott Lab | Gas valve |
US2834541A (en) * | 1956-09-20 | 1958-05-13 | Sorvall Inc Ivan | Centrifuging apparatus and system |
GB864410A (en) * | 1958-04-15 | 1961-04-06 | Sorvall Inc Ivan | Continuous centrifuge |
US3050238A (en) * | 1959-03-02 | 1962-08-21 | Dresser Ind | Liquid feed arrangement for centrifugal devices |
US3064646A (en) * | 1958-10-15 | 1962-11-20 | Galen L Miller | Breathing apparatus |
US3092106A (en) * | 1958-08-04 | 1963-06-04 | Cutter Lab | Administration equipment |
US3133880A (en) * | 1959-12-21 | 1964-05-19 | Vernon D Jarvis | Centrifugal extractor |
US3185154A (en) * | 1962-08-14 | 1965-05-25 | Joseph F Caccavo | Apparatus for collecting, separating, storing and dispensing whole blood |
US3235173A (en) * | 1960-07-28 | 1966-02-15 | Unger Hans Peter Olof | Agitating and/or fractioning centrifuge |
US3347454A (en) * | 1964-05-13 | 1967-10-17 | Baxter Laboratories Inc | Method and apparatus for the centrifugal washing of particles in a closed system |
-
1965
- 1965-02-03 US US430118A patent/US3452924A/en not_active Expired - Lifetime
-
1966
- 1966-01-28 GB GB4022/66A patent/GB1128303A/en not_active Expired
- 1966-01-29 DE DE1617782A patent/DE1617782C3/de not_active Expired
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2775240A (en) * | 1953-02-09 | 1956-12-25 | Abbott Lab | Gas valve |
US2758597A (en) * | 1955-01-24 | 1956-08-14 | Cutter Lab | Blood transfusion apparatus |
US2834541A (en) * | 1956-09-20 | 1958-05-13 | Sorvall Inc Ivan | Centrifuging apparatus and system |
GB864410A (en) * | 1958-04-15 | 1961-04-06 | Sorvall Inc Ivan | Continuous centrifuge |
US3092106A (en) * | 1958-08-04 | 1963-06-04 | Cutter Lab | Administration equipment |
US3064646A (en) * | 1958-10-15 | 1962-11-20 | Galen L Miller | Breathing apparatus |
US3050238A (en) * | 1959-03-02 | 1962-08-21 | Dresser Ind | Liquid feed arrangement for centrifugal devices |
US3133880A (en) * | 1959-12-21 | 1964-05-19 | Vernon D Jarvis | Centrifugal extractor |
US3235173A (en) * | 1960-07-28 | 1966-02-15 | Unger Hans Peter Olof | Agitating and/or fractioning centrifuge |
US3185154A (en) * | 1962-08-14 | 1965-05-25 | Joseph F Caccavo | Apparatus for collecting, separating, storing and dispensing whole blood |
US3347454A (en) * | 1964-05-13 | 1967-10-17 | Baxter Laboratories Inc | Method and apparatus for the centrifugal washing of particles in a closed system |
Cited By (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3977598A (en) * | 1973-05-24 | 1976-08-31 | Mcdonald Bernard | Centrifuge tube |
US3982691A (en) * | 1974-10-09 | 1976-09-28 | Schlutz Charles A | Centrifuge separation and washing device and method |
DE2612990A1 (de) * | 1975-03-27 | 1976-10-21 | Baxter Laboratories Inc | Stroemungssystem fuer die bearbeitung von zellen in einer zellbearbeitungszentrifuge |
US4049192A (en) * | 1976-11-01 | 1977-09-20 | Union Carbide Corporation | Blood washing method using a saline wash solution of varying concentration for use with blood washing apparatus |
US4069968A (en) * | 1976-11-01 | 1978-01-24 | Union Carbide Corporation | Disposable tubing harness for use with blood washing apparatus |
US4135883A (en) * | 1977-08-29 | 1979-01-23 | Bio-Dynamics Inc. | Blood analyzer system |
EP0072201A2 (de) * | 1981-08-05 | 1983-02-16 | Ortho Diagnostic Systems Inc. | Waschvorrichtung für Partikel und Verfahren zur Benutzung derselben |
EP0072201A3 (de) * | 1981-08-05 | 1985-05-22 | Ortho Diagnostic Systems Inc. | Waschvorrichtung für Partikel und Verfahren zur Benutzung derselben |
FR2559075A1 (fr) * | 1984-02-07 | 1985-08-09 | Stephan Nees | Procede et dispositif pour la separation de particules, systemes de cellules biologiques et colloides par centrifugation |
US4648863A (en) * | 1984-02-07 | 1987-03-10 | Edmund Buhler | Apparatus for the pure preparation of particles, biological cell systems and colloids |
US4804363A (en) * | 1986-07-16 | 1989-02-14 | Autologous Blood Corporation | Apparatus and method for storing and processing blood |
USRE33924E (en) * | 1986-07-16 | 1992-05-12 | Autologous Blood Corp. | Apparatus and method for storing and processing blood |
US5876321A (en) * | 1995-04-14 | 1999-03-02 | Cobe Laboratories, Inc. | Control system for the spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus |
US5704888A (en) * | 1995-04-14 | 1998-01-06 | Cobe Laboratories, Inc. | Intermittent collection of mononuclear cells in a centrifuge apparatus |
US5704889A (en) * | 1995-04-14 | 1998-01-06 | Cobe Laboratories, Inc. | Spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus |
US5879280A (en) * | 1995-04-14 | 1999-03-09 | Cobe Laboratories, Inc. | Intermittent collection of mononuclear cells in a centrifuge apparatus |
US6197579B1 (en) | 1997-02-14 | 2001-03-06 | Dendreon Corporation | Cell washing device and method |
US20070118063A1 (en) * | 2005-10-05 | 2007-05-24 | Gambro, Inc | Method and Apparatus for Leukoreduction of Red Blood Cells |
WO2008051847A2 (en) * | 2006-10-20 | 2008-05-02 | Caridianbct Biotechnologies, Llc | Methods for washing a red blood cell component and for removing prions therefrom |
US20080096750A1 (en) * | 2006-10-20 | 2008-04-24 | Navigant Biotechnologies, Llc | Methods for Washing a Red Blood Cell Component and for Removing Prions Therefrom |
WO2008051847A3 (en) * | 2006-10-20 | 2008-07-03 | Navigant Biotechnologies Llc | Methods for washing a red blood cell component and for removing prions therefrom |
US8016736B2 (en) | 2006-10-20 | 2011-09-13 | Caridianbct Biotechnologies, Llc | Methods for washing a red blood cell component and for removing prions therefrom |
US20080147240A1 (en) * | 2006-12-19 | 2008-06-19 | Gambro Bct Inc. | Apparatus for separating a composite liquid with process control on a centrifuge rotor |
EP2371943A1 (de) * | 2008-12-25 | 2011-10-05 | Olympus Corporation | Verfahren zum waschen von zellen |
EP2371943A4 (de) * | 2008-12-25 | 2012-06-13 | Olympus Corp | Verfahren zum waschen von zellen |
US20100291534A1 (en) * | 2009-05-12 | 2010-11-18 | National Central University | Methods and Systems for Isolating, Ex Vivo Expanding and Harvesting Hematopoietic Stem Cells |
CN113444628A (zh) * | 2021-05-28 | 2021-09-28 | 电子科技大学 | 一种组织块清洗装置及其清洗方法 |
CN113444628B (zh) * | 2021-05-28 | 2022-04-15 | 电子科技大学 | 一种组织块清洗装置及其清洗方法 |
Also Published As
Publication number | Publication date |
---|---|
DE1617782C3 (de) | 1973-09-13 |
DE1617782A1 (de) | 1971-04-08 |
DE1617782B2 (de) | 1973-02-22 |
GB1128303A (en) | 1968-09-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US3452924A (en) | System and method for washing blood and the like | |
US4296882A (en) | Centrifugal fluid processing device | |
US4668214A (en) | Method of washing red blood cells | |
US3672564A (en) | Rotary fluid seal and distribution means for centrifuges | |
JP3357369B2 (ja) | 遠心分離管及び適合器 | |
US5770069A (en) | Collapsible container for holding a fluid during a centrifugation operation | |
US4076169A (en) | Centrifuge separation and washing device and method | |
US4482342A (en) | Blood processing system for cell washing | |
US6652136B2 (en) | Method of simultaneous mixing of samples | |
US4091989A (en) | Continuous flow fractionation and separation device and method | |
US3145713A (en) | Method and apparatus for processing blood | |
US2822126A (en) | Continuous feed centrifuge | |
CA2418477C (en) | Method and apparatus for the concentration of fluid-borne pathogens | |
US4192250A (en) | Valve-centrifuge | |
AU2002305810A1 (en) | Method and apparatus for the concentration of fluid-borne pathogens | |
US3708111A (en) | Apparatus and method for gradient zonal centrifugation | |
US4157780A (en) | Disposable, rotatable, star-shaped enclosure for use with blood washing apparatus | |
DE19634413C2 (de) | Verfahren zur Sortierzentrifugation oder Sortierdurchflußzentrifugation und Vorrichtung zur Durchführung des Verfahrens | |
Kumar et al. | Bioseparation engineering | |
GB946532A (en) | Concentrating sludge by centrifugation | |
US3394880A (en) | Horizontal centrifugation | |
DE19860952A1 (de) | Verfahren zur effizienz- und/oder selektivitätsgesteigerten Sortierzentrifugation oder Sortierdurchflußzentrifugation und Vorrichtung zur Durchführung des Verfahrens | |
SU1712856A1 (ru) | Способ концентрировани биообъектов в суспензии | |
SU398283A1 (ru) | Ротор лабораторной центрифуги | |
SU573194A1 (ru) | Центрифуга дл гель-фильтрационного разделени биологических жидкостей |